LOCUS       NM_018979              10796 bp    mRNA    linear   PRI 20-FEB-2025
DEFINITION  Homo sapiens WNK lysine deficient protein kinase 1 (WNK1),
            transcript variant 1, mRNA.
ACCESSION   NM_018979
VERSION     NM_018979.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 10796)
  AUTHORS   Khoroshkin,M., Buyan,A., Dodel,M., Navickas,A., Yu,J., Trejo,F.,
            Doty,A., Baratam,R., Zhou,S., Lee,S.B., Joshi,T., Garcia,K.,
            Choi,B., Miglani,S., Subramanyam,V., Modi,H., Carpenter,C.,
            Markett,D., Corces,M.R., Mardakheh,F.K., Kulakovskiy,I.V. and
            Goodarzi,H.
  TITLE     Systematic identification of post-transcriptional regulatory
            modules
  JOURNAL   Nat Commun 15 (1), 7872 (2024)
   PUBMED   39251607
  REMARK    Erratum:[Nat Commun. 2024 Oct 28;15(1):9277. doi:
            10.1038/s41467-024-52903-4.. PMID: 39468017]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10796)
  AUTHORS   Li,L., Xie,D., Yu,S., Ma,M., Fan,K., Chen,J., Xiu,M., Xie,K., Li,Y.
            and Gao,Y.
  TITLE     WNK1 Interaction with KEAP1 Promotes NRF2 Stabilization to Enhance
            the Oxidative Stress Response in Hepatocellular Carcinoma
  JOURNAL   Cancer Res 84 (17), 2776-2791 (2024)
   PUBMED   38885324
  REMARK    GeneRIF: WNK1 Interaction with KEAP1 Promotes NRF2 Stabilization to
            Enhance the Oxidative Stress Response in Hepatocellular Carcinoma.
REFERENCE   3  (bases 1 to 10796)
  AUTHORS   Ma,Y., Zong,H., Pan,P., Shang,H. and Yang,X.
  TITLE     The CREB1/WNK1 axis promotes the tumorigenesis of ovarian cancer
            via regulating HIF-1
  JOURNAL   Exp Cell Res 438 (1), 114006 (2024)
   PUBMED   38599542
  REMARK    GeneRIF: The CREB1/WNK1 axis promotes the tumorigenesis of ovarian
            cancer via regulating HIF-1.
REFERENCE   4  (bases 1 to 10796)
  AUTHORS   Newhouse,S., Farrall,M., Wallace,C., Hoti,M., Burke,B., Howard,P.,
            Onipinla,A., Lee,K., Shaw-Hawkins,S., Dobson,R., Brown,M.,
            Samani,N.J., Dominiczak,A.F., Connell,J.M., Lathrop,G.M.,
            Kooner,J., Chambers,J., Elliott,P., Clarke,R., Collins,R., Laan,M.,
            Org,E., Juhanson,P., Veldre,G., Viigimaa,M., Eyheramendy,S.,
            Cappuccio,F.P., Ji,C., Iacone,R., Strazzullo,P., Kumari,M.,
            Marmot,M., Brunner,E., Caulfield,M. and Munroe,P.B.
  TITLE     Polymorphisms in the WNK1 gene are associated with blood pressure
            variation and urinary potassium excretion
  JOURNAL   PLoS One 4 (4), e5003 (2009)
   PUBMED   19347040
  REMARK    GeneRIF: data indicates that multiple rare and common WNK1 variants
            contribute to BP variation and hypertension, and provide compelling
            evidence to initiate further genetic and functional studies to
            explore the role of WNK1 in BP regulation and EH
            GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
REFERENCE   5  (bases 1 to 10796)
  AUTHORS   Shekarabi,M., Girard,N., Riviere,J.B., Dion,P., Houle,M.,
            Toulouse,A., Lafreniere,R.G., Vercauteren,F., Hince,P.,
            Laganiere,J., Rochefort,D., Faivre,L., Samuels,M. and Rouleau,G.A.
  TITLE     Mutations in the nervous system--specific HSN2 exon of WNK1 cause
            hereditary sensory neuropathy type II
  JOURNAL   J Clin Invest 118 (7), 2496-2505 (2008)
   PUBMED   18521183
  REMARK    GeneRIF: HSN2 is a nervous system-specific exon of the WNK1 gene
            (WNK1/HSN2 isoform).
REFERENCE   6  (bases 1 to 10796)
  AUTHORS   Disse-Nicodeme,S., Achard,J.M., Desitter,I., Houot,A.M.,
            Fournier,A., Corvol,P. and Jeunemaitre,X.
  TITLE     A new locus on chromosome 12p13.3 for pseudohypoaldosteronism type
            II, an autosomal dominant form of hypertension
  JOURNAL   Am J Hum Genet 67 (2), 302-310 (2000)
   PUBMED   10869238
REFERENCE   7  (bases 1 to 10796)
  AUTHORS   Moore,T.M., Garg,R., Johnson,C., Coptcoat,M.J., Ridley,A.J. and
            Morris,J.D.
  TITLE     PSK, a novel STE20-like kinase derived from prostatic carcinoma
            that activates the c-Jun N-terminal kinase mitogen-activated
            protein kinase pathway and regulates actin cytoskeletal
            organization
  JOURNAL   J Biol Chem 275 (6), 4311-4322 (2000)
   PUBMED   10660600
REFERENCE   8  (bases 1 to 10796)
  AUTHORS   Ellison,D.H.
  TITLE     Pseudohypoaldosteronism Type II
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   22073419
REFERENCE   9  (bases 1 to 10796)
  AUTHORS   Kurth,I.
  TITLE     Hereditary Sensory and Autonomic Neuropathy Type II
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   21089229
REFERENCE   10 (bases 1 to 10796)
  AUTHORS   Ota,M., Ellefson,R.D., Lambert,E.H. and Dyck,P.J.
  TITLE     Hereditary sensory neuropathy, type II. Clinical,
            electrophysiologic, histologic, and biochemical studies of a Quebec
            kinship
  JOURNAL   Arch Neurol 29 (1), 23-37 (1973)
   PUBMED   4351257
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC004765.2, AJ296290.1,
            CD629562.1, AB002342.2, BX644268.1, DA720959.1, AC004803.11,
            BC141881.1 and AI074334.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_018979.3.
            
            Summary: This gene encodes a member of the WNK subfamily of
            serine/threonine protein kinases. The encoded protein may be a key
            regulator of blood pressure by controlling the transport of sodium
            and chloride ions. Mutations in this gene have been associated with
            pseudohypoaldosteronism type II and hereditary sensory neuropathy
            type II. Alternatively spliced transcript variants encoding
            different isoforms have been described but the full-length nature
            of all of them has yet to be determined.[provided by RefSeq, May
            2010].
            
            Transcript Variant: This variant (1) encodes the most common
            isoform (1), as indicated in PubMed ID 18521183.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AJ296290.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000315939.11/ ENSP00000313059.6
            RefSeq Select criteria :: based on expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1263              AC004765.2         22013-23275
            1264-2207           AJ296290.1         277-1220
            2208-2327           CD629562.1         188-307
            2328-3210           AB002342.2         1139-2021
            3211-4152           AJ296290.1         2224-3165
            4153-4501           BX644268.1         276-624
            4502-4936           DA720959.1         133-567
            4937-6267           AC004803.11        5594-6924
            6268-7814           BC141881.1         2748-4294
            7815-8136           AJ296290.1         6828-7149
            8137-10419          AC004803.11        29633-31915
            10420-10796         AI074334.1         10-386              c
FEATURES             Location/Qualifiers
     source          1..10796
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="12"
                     /map="12p13.33"
     gene            1..10796
                     /gene="WNK1"
                     /gene_synonym="HSAN2; HSN2; KDP; p65; PPP1R167; PRKWNK1;
                     PSK"
                     /note="WNK lysine deficient protein kinase 1"
                     /db_xref="GeneID:65125"
                     /db_xref="HGNC:HGNC:14540"
                     /db_xref="MIM:605232"
     exon            1..1746
                     /gene="WNK1"
                     /gene_synonym="HSAN2; HSN2; KDP; p65; PPP1R167; PRKWNK1;
                     PSK"
                     /inference="alignment:Splign:2.1.0"
     CDS             988..8136
                     /gene="WNK1"
                     /gene_synonym="HSAN2; HSN2; KDP; p65; PPP1R167; PRKWNK1;
                     PSK"
                     /EC_number="2.7.11.1"
                     /note="isoform 1 is encoded by transcript variant 1;
                     prostate-derived sterile 20-like kinase; erythrocyte 65
                     kDa protein; protein kinase with no lysine 1; protein
                     phosphatase 1, regulatory subunit 167;
                     serine/threonine-protein kinase WNK1 1;
                     serine/threonine-protein kinase WNK1 2"
                     /codon_start=1
                     /product="serine/threonine-protein kinase WNK1 isoform 1"
                     /protein_id="NP_061852.3"
                     /db_xref="CCDS:CCDS8506.1"
                     /db_xref="GeneID:65125"
                     /db_xref="HGNC:HGNC:14540"
                     /db_xref="MIM:605232"
                     /translation="MSGGAAEKQSSTPGSLFLSPPAPAPKNGSSSDSSVGEKLGAAAA
                     DAVTGRTEEYRRRRHTMDKDSRGAAATTTTTEHRFFRRSVICDSNATALELPGLPLSL
                     PQPSIPAAVPQSAPPEPHREETVTATATSQVAQQPPAAAAPGEQAVAGPAPSTVPSST
                     SKDRPVSQPSLVGSKEEPPPARSGSGGGSAKEPQEERSQQQDDIEELETKAVGMSNDG
                     RFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQ
                     HPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKG
                     LQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAP
                     EMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIP
                     EVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIED
                     IKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIK
                     RKREQRQLVREEQEKKKQEESSLKQQVEQSSASQTGIKQLPSASTGIPTASTTSASVS
                     TQVEPEEPEADQHQQLQYQQPSISVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHE
                     QAHSTGTVPGHIPSTVQAQSQPHGVYPPSSVAQGQSQGQPSSSSLTGVSSSQPIQHPQ
                     QQQGIQQTAPPQQTVQYSLSQTSTSSEATTAQPVSQPQAPQVLPQVSAGKQLPVSQPV
                     PTIQGEPQIPVATQPSVVPVHSGAHFLPVGQPLPTPLLPQYPVSQIPISTPHVSTAQT
                     GFSSLPITMAAGITQPLLTLASSATTAAIPGVSTVVPSQLPTLLQPVTQLPSQVHPQL
                     LQPAVQSMGIPANLGQAAEVPLSSGDVLYQGFPPRLPPQYPGDSNIAPSSNVASVCIH
                     STVLSPPMPTEVLATPGYFPTVVQPYVESNLLVPMGGVGGQVQVSQPGGSLAQAPTTS
                     SQQAVLESTQGVSQVAPAEPVAVAQTQATQPTTLASSVDSAHSDVASGMSDGNENVPS
                     SSGRHEGRTTKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVT
                     FKFDLDGDNPEEIATIMVNNDFILAIERESFVDQVREIIEKADEMLSEDVSVEPEGDQ
                     GLESLQGKDDYGFSGSQKLEGEFKQPIPASSMPQQIGIPTSSLTQVVHSAGRRFIVSP
                     VPESRLRESKVFPSEITDTVAASTAQSPGMNLSHSASSLSLQQAFSELRRAQMTEGPN
                     TAPPNFSHTGPTFPVVPPFLSSIAGVPTTAAATAPVPATSSPPNDISTSVIQSEVTVP
                     TEEGIAGVATSTGVVTSGGLPIPPVSESPVLSSVVSSITIPAVVSISTTSPSLQVPTS
                     TSEIVVSSTALYPSVTVSATSASAGGSTATPGPKPPAVVSQQAAGSTTVGATLTSVST
                     TTSFPSTASQLCIQLSSSTSTPTLAETVVVSAHSLDKTSHSSTTGLAFSLSAPSSSSS
                     PGAGVSSYISQPGGLHPLVIPSVIASTPILPQAAGPTSTPLLPQVPSIPPLVQPVANV
                     PAVQQTLIHSQPQPALLPNQPHTHCPEVDSDTQPKAPGIDDIKTLEEKLRSLFSEHSS
                     SGAQHASVSLETSLVIESTVTPGIPTTAVAPSKLLTSTTSTCLPPTNLPLGTVALPVT
                     PVVTPGQVSTPVSTTTSGVKPGTAPSKPPLTKAPVLPVGTELPAGTLPSEQLPPFPGP
                     SLTQSQQPLEDLDAQLRRTLSPEMITVTSAVGPVSMAAPTAITEAGTQPQKGVSQVKE
                     GPVLATSSGAGVFKMGRFQVSVAADGAQKEGKNKSEDAKSVHFESSTSESSVLSSSSP
                     ESTLVKPEPNGITIPGISSDVPESAHKTTASEAKSDTGQPTKVGRFQVTTTANKVGRF
                     SVSKTEDKITDTKKEGPVASPPFMDLEQAVLPAVIPKKEKPELSEPSHLNGPSSDPEA
                     AFLSRDVDDGSGSPHSPHQLSSKSLPSQNLSQSLSNSFNSSYMSSDNESDIEDEDLKL
                     ELRRLRDKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKG
                     SKSSRSSSLGNKSPQLSGNLSGQSAASVLHPQQTLHPPGNIPESGQNQLLQPLKPSPS
                     SDNLYSAFTSDGAISVPSLSAPGQGTSSTNTVGATVNSQAAQAQPPAMTSSRKGTFTD
                     DLHKLVDNWARDAMNLSGRRGSKGHMNYEGPGMARKFSAPGQLCISMTSNLGGSAPIS
                     AASATSLGHFTKSMCPPQQYGFPATPFGAQWSGTGGPAPQPLGQFQPVGTASLQNFNI
                     SNLQKSISNPPGSNLRTT"
     exon            1747..1919
                     /gene="WNK1"
                     /gene_synonym="HSAN2; HSN2; KDP; p65; PPP1R167; PRKWNK1;
                     PSK"
                     /inference="alignment:Splign:2.1.0"
     exon            1920..2140
                     /gene="WNK1"
                     /gene_synonym="HSAN2; HSN2; KDP; p65; PPP1R167; PRKWNK1;
                     PSK"
                     /inference="alignment:Splign:2.1.0"
     exon            2141..2298
                     /gene="WNK1"
                     /gene_synonym="HSAN2; HSN2; KDP; p65; PPP1R167; PRKWNK1;
                     PSK"
                     /inference="alignment:Splign:2.1.0"
     exon            2299..2387
                     /gene="WNK1"
                     /gene_synonym="HSAN2; HSN2; KDP; p65; PPP1R167; PRKWNK1;
                     PSK"
                     /inference="alignment:Splign:2.1.0"
     exon            2388..2607
                     /gene="WNK1"
                     /gene_synonym="HSAN2; HSN2; KDP; p65; PPP1R167; PRKWNK1;
                     PSK"
                     /inference="alignment:Splign:2.1.0"
     exon            2608..2938
                     /gene="WNK1"
                     /gene_synonym="HSAN2; HSN2; KDP; p65; PPP1R167; PRKWNK1;
                     PSK"
                     /inference="alignment:Splign:2.1.0"
     exon            2939..3126
                     /gene="WNK1"
                     /gene_synonym="HSAN2; HSN2; KDP; p65; PPP1R167; PRKWNK1;
                     PSK"
                     /inference="alignment:Splign:2.1.0"
     exon            3127..3210
                     /gene="WNK1"
                     /gene_synonym="HSAN2; HSN2; KDP; p65; PPP1R167; PRKWNK1;
                     PSK"
                     /inference="alignment:Splign:2.1.0"
     exon            3211..3360
                     /gene="WNK1"
                     /gene_synonym="HSAN2; HSN2; KDP; p65; PPP1R167; PRKWNK1;
                     PSK"
                     /inference="alignment:Splign:2.1.0"
     exon            3361..3819
                     /gene="WNK1"
                     /gene_synonym="HSAN2; HSN2; KDP; p65; PPP1R167; PRKWNK1;
                     PSK"
                     /inference="alignment:Splign:2.1.0"
     exon            3820..4098
                     /gene="WNK1"
                     /gene_synonym="HSAN2; HSN2; KDP; p65; PPP1R167; PRKWNK1;
                     PSK"
                     /inference="alignment:Splign:2.1.0"
     exon            4099..4196
                     /gene="WNK1"
                     /gene_synonym="HSAN2; HSN2; KDP; p65; PPP1R167; PRKWNK1;
                     PSK"
                     /inference="alignment:Splign:2.1.0"
     exon            4197..4359
                     /gene="WNK1"
                     /gene_synonym="HSAN2; HSN2; KDP; p65; PPP1R167; PRKWNK1;
                     PSK"
                     /inference="alignment:Splign:2.1.0"
     exon            4360..4476
                     /gene="WNK1"
                     /gene_synonym="HSAN2; HSN2; KDP; p65; PPP1R167; PRKWNK1;
                     PSK"
                     /inference="alignment:Splign:2.1.0"
     exon            4477..4650
                     /gene="WNK1"
                     /gene_synonym="HSAN2; HSN2; KDP; p65; PPP1R167; PRKWNK1;
                     PSK"
                     /inference="alignment:Splign:2.1.0"
     exon            4651..4708
                     /gene="WNK1"
                     /gene_synonym="HSAN2; HSN2; KDP; p65; PPP1R167; PRKWNK1;
                     PSK"
                     /inference="alignment:Splign:2.1.0"
     exon            4709..4831
                     /gene="WNK1"
                     /gene_synonym="HSAN2; HSN2; KDP; p65; PPP1R167; PRKWNK1;
                     PSK"
                     /inference="alignment:Splign:2.1.0"
     exon            4832..6267
                     /gene="WNK1"
                     /gene_synonym="HSAN2; HSN2; KDP; p65; PPP1R167; PRKWNK1;
                     PSK"
                     /inference="alignment:Splign:2.1.0"
     exon            6268..6351
                     /gene="WNK1"
                     /gene_synonym="HSAN2; HSN2; KDP; p65; PPP1R167; PRKWNK1;
                     PSK"
                     /inference="alignment:Splign:2.1.0"
     exon            6352..6435
                     /gene="WNK1"
                     /gene_synonym="HSAN2; HSN2; KDP; p65; PPP1R167; PRKWNK1;
                     PSK"
                     /inference="alignment:Splign:2.1.0"
     exon            6436..6496
                     /gene="WNK1"
                     /gene_synonym="HSAN2; HSN2; KDP; p65; PPP1R167; PRKWNK1;
                     PSK"
                     /inference="alignment:Splign:2.1.0"
     exon            6497..6570
                     /gene="WNK1"
                     /gene_synonym="HSAN2; HSN2; KDP; p65; PPP1R167; PRKWNK1;
                     PSK"
                     /inference="alignment:Splign:2.1.0"
     exon            6571..7232
                     /gene="WNK1"
                     /gene_synonym="HSAN2; HSN2; KDP; p65; PPP1R167; PRKWNK1;
                     PSK"
                     /inference="alignment:Splign:2.1.0"
     exon            7233..7435
                     /gene="WNK1"
                     /gene_synonym="HSAN2; HSN2; KDP; p65; PPP1R167; PRKWNK1;
                     PSK"
                     /inference="alignment:Splign:2.1.0"
     exon            7436..7630
                     /gene="WNK1"
                     /gene_synonym="HSAN2; HSN2; KDP; p65; PPP1R167; PRKWNK1;
                     PSK"
                     /inference="alignment:Splign:2.1.0"
     exon            7631..7818
                     /gene="WNK1"
                     /gene_synonym="HSAN2; HSN2; KDP; p65; PPP1R167; PRKWNK1;
                     PSK"
                     /inference="alignment:Splign:2.1.0"
     exon            7819..10796
                     /gene="WNK1"
                     /gene_synonym="HSAN2; HSN2; KDP; p65; PPP1R167; PRKWNK1;
                     PSK"
                     /inference="alignment:Splign:2.1.0"
     regulatory      8960..8965
                     /regulatory_class="polyA_signal_sequence"
                     /gene="WNK1"
                     /gene_synonym="HSAN2; HSN2; KDP; p65; PPP1R167; PRKWNK1;
                     PSK"
                     /note="hexamer: AATAAA"
     polyA_site      8983
                     /gene="WNK1"
                     /gene_synonym="HSAN2; HSN2; KDP; p65; PPP1R167; PRKWNK1;
                     PSK"
     regulatory      10563..10568
                     /regulatory_class="polyA_signal_sequence"
                     /gene="WNK1"
                     /gene_synonym="HSAN2; HSN2; KDP; p65; PPP1R167; PRKWNK1;
                     PSK"
                     /note="hexamer: ATTAAA"
     polyA_site      10586
                     /gene="WNK1"
                     /gene_synonym="HSAN2; HSN2; KDP; p65; PPP1R167; PRKWNK1;
                     PSK"
     regulatory      10772..10777
                     /regulatory_class="polyA_signal_sequence"
                     /gene="WNK1"
                     /gene_synonym="HSAN2; HSN2; KDP; p65; PPP1R167; PRKWNK1;
                     PSK"
                     /note="hexamer: AATAAA"
     polyA_site      10796
                     /gene="WNK1"
                     /gene_synonym="HSAN2; HSN2; KDP; p65; PPP1R167; PRKWNK1;
                     PSK"
                     /note="major polyA site"
ORIGIN      
        1 agactcccgg cgccatttag cgcggagagt ttcccgggtg gacgcggctc ctctctcggc
       61 cactccgcac ccccatcttc ggtgacagaa ggcgcctggt gggggtggct gctcttttct
      121 ctccctgttc cccctcaccc agtcctctag gtctcctctc ctcttgcctc agagaagcag
      181 cggagctcgg gccccgcggt gagcggccct cccctccccg ccgttccctc ctccgtcagc
      241 ccccggcacc ggcccgggag gagacgggtt tgccaggcct ggggcgggcg gggaggcctc
      301 ggggaagggg gggcccgctc ctcaggcgcc gaggctccga ggctccggcc cttcgcctct
      361 gggcgatggg cgacctgtga ggccggtccc catcgctggg ggcgcgtgtg ggaggaggcg
      421 gccgcccgag tgaccgggag ccgggccgcg gccttccctc gcccgcctcg gcccctccca
      481 ctcctctgcc ccggggccgc caccgcccgg gcgtcggacc tggtcccgtg ctcgcggtgc
      541 cgccgccctc tgggcctagc ccgcccagct cggcgagcgg cggcagtggg agccgcgtcc
      601 gccgcatccg cctcgactcg gtgccggccc ctggccctcc cctcatgact gcggcgcctc
      661 tgctgccacc gcccgcccgg ccgccgctcg ccgcaggatg gatgcggacc gtgcggcgct
      721 aacccccgtg gctcagctcc cgaatcgccc gccttcgagc cctcctcgtg agccgcagca
      781 gcctcggtgc cagcccccgc cgcagctggg cccagcggtc cgcctgtccc tcgttgcggc
      841 ttgtcggtgc tgagtgaggc gtcgtccggg tcggcgcgaa cccgcccggc cgcggttccc
      901 tgcagacctc tgcgcgggcg gctcggccct tcacgccctt ttcgttcacg aatccgagcc
      961 cgctcgcctc tctccagcga accgaccatg tctggcggcg ccgcagagaa gcagagcagc
     1021 actcccggtt ccctgttcct ctcgccgccg gctcctgccc ccaagaatgg ctccagctcc
     1081 gattcctccg tgggggagaa actgggagcc gcggccgccg acgctgtgac cggcaggacc
     1141 gaggagtaca ggcgccgccg ccacactatg gacaaggaca gccgtggggc ggccgcgacc
     1201 actaccacca ctgagcaccg cttcttccgc cggagcgtca tctgtgactc caatgccact
     1261 gcactggagc ttcccggcct tcctctttcc ctgccccagc ccagcatccc cgcggctgtc
     1321 ccgcagagtg ctccaccgga gccccaccgg gaagagaccg tgaccgccac cgccacttcc
     1381 caggtagccc agcagcctcc agccgctgcc gcccctgggg aacaggccgt cgcgggccct
     1441 gccccctcga ctgtccccag cagtaccagc aaagaccgcc cagtgtccca gcctagcctt
     1501 gtggggagca aagaggagcc gccgccggcg agaagtggca gcggcggcgg cagcgccaag
     1561 gagccacagg aggaacggag ccagcagcag gatgatatcg aagagctgga gaccaaggcc
     1621 gtgggaatgt ctaacgatgg ccgctttctc aagtttgaca tcgaaatcgg cagaggctcc
     1681 tttaagacgg tctacaaagg tctggacact gaaaccaccg tggaagtcgc ctggtgtgaa
     1741 ctgcaggatc gaaaattaac aaagtctgag aggcagagat ttaaagaaga agctgaaatg
     1801 ttaaaaggtc ttcagcatcc caatattgtt agattttatg attcctggga atccacagta
     1861 aaaggaaaga agtgcattgt tttggtgact gaacttatga cgtctggaac acttaaaacg
     1921 tatctgaaaa ggtttaaagt gatgaagatc aaagttctaa gaagctggtg ccgtcagatc
     1981 cttaaaggtc ttcagtttct tcatactcga actccaccta tcattcaccg cgatcttaaa
     2041 tgtgacaaca tctttatcac cggccctact ggctcagtca agattggaga cctcggtctg
     2101 gcaaccctga agcgggcttc ttttgccaag agtgtgatag gtaccccaga gttcatggcc
     2161 cctgagatgt atgaggagaa atatgatgaa tccgttgacg tttatgcttt tgggatgtgc
     2221 atgcttgaga tggctacatc tgaatatcct tactcggagt gccaaaatgc tgcacagatc
     2281 taccgtcgcg tgaccagtgg ggtgaagcca gccagttttg acaaagtagc aattcctgaa
     2341 gtgaaggaaa ttattgaagg atgcatacga caaaacaaag atgaaagata ttccatcaaa
     2401 gaccttttga accatgcctt cttccaagag gaaacaggag tacgggtaga attagcagaa
     2461 gaagatgatg gagaaaaaat agccataaaa ttatggctac gtattgaaga tattaagaaa
     2521 ttaaagggaa aatacaaaga taatgaagct attgagtttt cttttgattt agagagagat
     2581 gtcccagaag atgttgcaca agaaatggta gagtctgggt atgtctgtga aggtgatcac
     2641 aagaccatgg ctaaagctat caaagacaga gtatcattaa ttaagaggaa acgagagcag
     2701 cggcagttgg tacgggagga gcaagaaaaa aaaaagcagg aagagagcag tctcaaacag
     2761 caggtagaac aatccagtgc ttcccagaca ggaatcaagc agctcccttc tgctagcacc
     2821 ggcataccta ctgcttctac cacttcagct tcagtttcta cacaagtaga acctgaagaa
     2881 cctgaggcag atcaacatca acaactacag taccagcaac ccagtatatc tgtgttatct
     2941 gatgggacgg ttgacagtgg tcagggatcc tctgtcttca cagaatctcg agtgagcagc
     3001 caacagacag tttcatatgg ttcccaacat gaacaggcac attctacagg cacagtccca
     3061 gggcatatac cttctactgt ccaagcacag tctcagcccc atggggtata tccaccctca
     3121 agtgtggcac aggggcagag ccagggtcag ccatcctcaa gtagcttaac aggggtttca
     3181 tcttcccaac ccatacaaca tcctcagcag cagcagggaa tacagcagac agcccctcct
     3241 caacagacag tgcagtattc actttcacag acatcaacct ccagtgaggc cactactgca
     3301 cagccagtga gtcagcctca agctccacaa gtcttgcctc aagtatcagc tggaaaacag
     3361 cttccagttt cccagccagt accaactatc caaggcgaac ctcagatccc agttgcgaca
     3421 caaccctcgg ttgttccagt ccactctggt gctcatttcc ttccagtggg acagccgctc
     3481 cctactccct tgctccctca gtaccctgtc tctcagattc ccatatcaac tcctcatgtg
     3541 tctacggctc agacaggttt ctcatccctt cccatcacaa tggcagctgg cattactcag
     3601 cctctgctca cgttggcttc atctgctaca acagctgcga tcccgggggt atcaactgtg
     3661 gttcctagtc agcttccaac ccttctgcag cctgtgactc agctgccaag tcaggttcac
     3721 ccacagctcc tacaaccagc agttcagtcc atgggaatac cagctaacct tggacaagct
     3781 gctgaggttc cactttcctc tggagatgtt ctgtaccagg gcttcccacc tcgactgcca
     3841 ccacagtacc caggagattc aaatattgct ccctcttcca acgtggcttc tgtttgcatc
     3901 cattctacag tcctatcccc tcccatgccg acagaagtac tggctacacc tgggtacttt
     3961 cccacagtgg tgcagcctta tgtggaatca aatcttttag ttcctatggg tggtgtagga
     4021 ggacaggttc aagtgtccca gccaggaggg agtttagcac aagcccccac tacatcctcc
     4081 cagcaagcag ttttggagag tactcaggga gtctctcagg ttgctcctgc agagccagtt
     4141 gcagtagcac agacccaagc tacccagccg accactttgg cttcctctgt agacagtgca
     4201 cattcagatg ttgcttcagg tatgagtgat ggcaatgaga acgtcccatc ttccagtgga
     4261 aggcatgaag gaagaactac aaaacggcat taccgaaaat ctgtaaggag tcgctctcga
     4321 catgaaaaaa cttcacgccc aaaattaaga attttgaatg tttcaaataa aggagaccga
     4381 gtagtagaat gtcaattaga gactcataat aggaaaatgg ttacattcaa atttgaccta
     4441 gatggtgaca accccgagga gatagcaaca attatggtga acaatgactt tattctagca
     4501 atagagagag agtcgtttgt ggatcaagtg cgagaaatta ttgaaaaagc tgatgaaatg
     4561 ctcagtgagg atgtcagtgt ggaaccagag ggtgatcagg gattggagag tctacaagga
     4621 aaggatgact atggcttttc aggttctcag aaattggaag gagagttcaa acaaccaatt
     4681 cctgcgtctt ccatgccaca gcaaataggc attcctacca gttctttaac tcaagttgtt
     4741 cattctgcgg gaaggcggtt tatagtgagt cctgtgccag aaagccgatt acgagaatca
     4801 aaagttttcc ccagtgaaat aacagataca gttgctgcct ctacagctca gagccctgga
     4861 atgaacttgt ctcactctgc atcatccctt agtctacaac aggccttttc tgaacttaga
     4921 cgtgcccaaa tgacagaagg acccaacaca gcacctccaa actttagtca tacaggacca
     4981 acatttccag tagtacctcc tttcttaagt agcattgctg gagtcccaac cacagcagca
     5041 gccacagcac cagtccctgc aacaagcagc cctcctaatg acatttccac atcagtaatt
     5101 cagtctgagg ttacagtgcc cactgaagag gggattgctg gagttgccac cagcacaggt
     5161 gtggtaactt caggtggtct ccccatacca cctgtgtctg aatcaccagt actttccagc
     5221 gtagtttcaa gtatcacaat acctgcagtt gtctcaatat ctactacatc cccgtcactt
     5281 caagtcccca catccacatc tgagatcgtt gtttctagta cagcactgta tccttcagta
     5341 acagtttcag caacttcagc ctctgcaggg ggcagtactg ctaccccagg tcctaagcct
     5401 ccagctgtag tatctcagca ggcagcaggc agcactactg tgggagccac attaacatca
     5461 gtttctacca ccacttcatt cccaagcaca gcttcacagc tgtgcattca gcttagcagc
     5521 agtacttcta ctcctacttt agctgaaacc gtggtagtta gcgcacactc actagataag
     5581 acatctcata gcagtacaac tggattggct ttctccctct ctgcaccatc ttcctcttcc
     5641 tctcctggag caggagtgtc tagttatatt tctcagcctg gtgggctgca tcctttggtc
     5701 attccatcag tgatagcttc tactcctatt cttccccaag cagcaggacc tacttctaca
     5761 cctttattac cccaagtacc tagtatccca cccttggtac agcctgttgc caatgtgcct
     5821 gctgtacagc agacactaat tcatagtcag cctcaaccag ctttgcttcc caaccagccc
     5881 catactcatt gtcctgaagt agattctgat acacaaccca aagctcctgg aattgatgac
     5941 ataaagactc tagaagaaaa gctgcggtct ctgttcagtg aacacagctc atctggagct
     6001 cagcatgcct ctgtctcact ggagacctca ctagtcatag agagcactgt cacaccaggc
     6061 atcccaacta ctgctgttgc accaagcaaa ctcctgactt ctaccacaag tacttgctta
     6121 ccaccaacca atttaccact aggaacagtt gctttgccag ttacaccagt ggtcacacct
     6181 gggcaagttt ctaccccagt cagcactact acatcaggag tgaaacctgg aactgctccc
     6241 tccaagccac ctctaactaa ggctccggtg ctgccagtgg gtactgaact tccagcaggt
     6301 actctaccca gcgagcagct gccacctttt ccaggacctt ctctaaccca gtcccagcaa
     6361 cctctagagg atcttgatgc tcaattgaga agaacactta gtccagagat gatcacagtg
     6421 acttctgcgg ttggtcctgt gtccatggcg gctccaacag caatcacaga agcaggaaca
     6481 cagcctcaga agggtgtttc tcaagtcaaa gaaggccctg tcctagcaac tagttcagga
     6541 gctggtgttt ttaagatggg acgatttcag gtttctgttg cagcagacgg tgcccagaaa
     6601 gagggtaaaa ataagtcaga agatgcaaag tctgttcatt ttgaatccag cacctcagag
     6661 tcctcagtgc tatcaagtag tagtccagag agtaccttgg tgaaaccaga gccgaatggc
     6721 ataaccatcc ctggtatctc ttcagatgtg ccagagagtg cccacaaaac tactgcctca
     6781 gaggcaaagt cagacactgg gcagcctacc aaggttggac gttttcaggt gacaactaca
     6841 gcaaacaaag tgggtcgttt ctctgtatca aaaactgagg acaagatcac tgacacaaag
     6901 aaagaaggac cagtggcatc tcctcctttt atggatttgg aacaagctgt tcttcctgct
     6961 gtgataccaa agaaagagaa gcctgaactg tcagagcctt cacatctaaa tgggccgtct
     7021 tctgacccgg aggccgcttt tttaagtagg gatgtggatg atggttccgg tagtccacac
     7081 tcgccccatc agctgagctc aaagagcctt cctagccaga atctaagtca aagccttagt
     7141 aattcattta actcctctta catgagtagc gacaatgagt cagatatcga agatgaagac
     7201 ttaaagttag agctgcgacg actacgagat aaacatctca aagagattca ggacctgcag
     7261 agtcgccaga agcatgaaat tgaatctttg tataccaaac tgggcaaggt gccccctgct
     7321 gttattattc ccccagctgc tcccctttca gggagaagac gacgacccac taaaagcaaa
     7381 ggcagcaaat ctagtcgaag cagttccttg gggaataaaa gcccccagct ttcaggtaac
     7441 ctgtctggtc agagtgcagc ttcagtcttg cacccccagc agaccctcca ccctcctggc
     7501 aacatcccag agtccgggca gaatcagctg ttacagcccc ttaagccatc tccctccagt
     7561 gacaacctct attcagcctt caccagtgat ggtgccattt cagtaccaag cctttctgct
     7621 ccaggtcaag gaaccagcag cacaaacact gttggggcaa cagtgaacag ccaagccgcc
     7681 caagctcagc ctcctgccat gacgtccagc aggaagggca cattcacaga tgacttgcac
     7741 aagttggtag acaattgggc ccgagatgcc atgaatctct caggcaggag aggaagcaaa
     7801 gggcacatga attacgaggg ccctggaatg gcaaggaagt tctctgcacc tgggcaactg
     7861 tgcatctcca tgacctcgaa cctgggtggc tctgccccca tctctgcagc atcagctacc
     7921 tctctaggtc acttcaccaa gtctatgtgc cccccacagc agtatggctt tccagctacc
     7981 ccatttggcg ctcaatggag tgggacgggt ggcccagcac cacagccact tggccagttc
     8041 caacctgtgg gaactgcctc cttgcagaat ttcaacatca gcaatttgca gaaatccatc
     8101 agcaaccccc caggctccaa cctgcggacc acttagacct agagacatta actgaataga
     8161 tctgggggca ggagatggaa tgctgagggg gtgggtgggg gtgggaagta gcctatatac
     8221 taactactag tgctgcattt aactggttat ttcttgccag aggggaatgt ttttaatact
     8281 gcattgagcc ctcagaatgg agagtctccc ccgctccagt tattggaatg ggagaggaag
     8341 gaaagaacag cttttttgtc aaggggcagc ttcagaccat gctttcctgt ttatctatac
     8401 tcagtaatga ggatgagggc taggaaagtc ttgttcataa ggaagctgga gaactcaatg
     8461 taaaatcaaa cccatctgta atttcgagtg ggtggagctc ttgcttttgg tacatgccct
     8521 gaatccctca ctccctcaag aatccgaacc acaggacaaa aaccacctac tgggctctct
     8581 cctaccctgc cctcctccct tttttttacc cctctctttt ttattttttc tttgctcttt
     8641 agaacccagt gaaaaatacc agggtactgg ggtgcaactc tttcttatga taggtcatta
     8701 gtgctttaag caaaagatat tagcagcttt gactgcagca ttagcaatta ggaaaaaaaa
     8761 aaaattaagt tccctgcgga catgtaactt tgccatcagt tttgatgtgg aaacactgtg
     8821 atatataaaa tgttgttgga caacagtagt tttaagagta aaatatgaaa cgtttaaaaa
     8881 gttccaaaaa aagctagctc tgtcctttac ttattgagac actttaactt tttcctttgt
     8941 atttccattg tattagataa ataaatgtga atgtaaaatt gtataaatta ctgtacttga
     9001 atacttctgt ttcccagtgt tgcttgctgg acattttagt gccttggact tctattgctt
     9061 ctgccattag catcaactta ccagacccca gatcaataaa gggcatgtgg aaggaaatcg
     9121 taggtccatg tgaccccagc agtccagcag tggttatgcc aaagggaaat tgaaaaagta
     9181 tttttttaag tcattcaaca actttgtcta gagcaggtgt aagatgagta gggtgggaag
     9241 ttaggttggc atcagtggtt aaaaacagaa agttctgttt cgggaatagt gaggaggggg
     9301 tgttgtaaca aaattggaca acttaaaaga atggtgtgtg ctgggtgaaa gacaaagact
     9361 aaagaatgag gaaacaaacg tgatgcctgg ccagtgactg tcatataaac ctttcttatt
     9421 tgagctaggc ttgaacagac gtgacctaga agaaactgaa cataaagaga agggggtggg
     9481 gggctagttt tcaagttggg gaacctgata gtgaaaagtc acagatggag aaaattgctc
     9541 tcagaaaaac tgtttggatt gctttcctct tgttgcacat gtaccatgca tttctcagct
     9601 tggggtacta cattttgtgg aaagttaatc tatctatctt tccacatctg aattaatcat
     9661 tctaggaaag aatacttatt cctactcatt tcctttatga tgtccaaatg gttgcaggat
     9721 cataatctat tgtgccacct ttatttctag aagtacaact aatatgttca cattttcaaa
     9781 taaataatac tccccgtaag taataactgc aaccaatcag tgttattcag tgctatgcct
     9841 ccttgtaatg ggtagttatt aattattttc agagctttcc ggaaatactg tcctaactgg
     9901 ctatgtttag gatctttgtt atctctgaag acaaagaaag aagctaggac tcttaatttt
     9961 ggggtgcttc ttgactctta gttgggaaac tgaaaatatt tccaaccttt tacccacgtc
    10021 aatggcatat tctgggaatc accaccacca ccaccactac cacagaaaga ggctggaggc
    10081 tcctgtaccc tgttcattcc ttaagggccc tgcttccctt agtaagtaag taagttggtc
    10141 tacggcccta aatatgcaaa tgagagctga aggtttttaa aaggtagaaa ggaaaagggc
    10201 aagggcttcc acccctgctt taaaatgatt tatttattct ctgcttgtat ttcttgtgga
    10261 gagagtaagg atagaaccaa caaggggctg agtagctgag aaaggggcca cccaagagtg
    10321 aaacatactt tataccagag gagcagtgga gcctcatgca gcacattatc atttgttatt
    10381 tgggtttaat aataattttg acatcttttc actcatacac aaaaaaagtc agaactggtg
    10441 ttatttactg ttgatttcat cctcctgtgt atgaaataac aagcctagag gaatgaacta
    10501 gtgctactga actgtttaaa ttatttttgt gttaatagta cactttgagt atctttttcc
    10561 acattaaaaa ctttctgaat tataaatgtt ttccttacat tatttaacaa tgtacactgt
    10621 taaaaataaa aataaaaatt caaactttgg gggtttctca gcagccgtta attgtacatt
    10681 ttgcactaac tctgggtgtt gcgcttcttg taagattgcg ctttgtgctt cagtttgtta
    10741 cctttgtaga cttatttaat gaaaccattc aaataaacca aacttgcttt tgttga
//
