LOCUS       NM_001193334            2482 bp    mRNA    linear   PRI 03-APR-2024
DEFINITION  Homo sapiens Wnt ligand secretion mediator (WLS), transcript
            variant 3, mRNA.
ACCESSION   NM_001193334
VERSION     NM_001193334.1
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2482)
  AUTHORS   Abdel-Salam,G.M.H., Afifi,H.H., Abdel-Hamid,M.S., Ahmed,N.E.B.,
            Taher,M.B., El-Kamah,G., Thiele,H., Nurnberg,P.N. and Bolz,H.J.
  TITLE     Expanding the phenotypic spectrum and clinical severity associated
            with WLS gene
  JOURNAL   J Hum Genet 68 (9), 607-613 (2023)
   PUBMED   37106064
  REMARK    GeneRIF: Expanding the phenotypic spectrum and clinical severity
            associated with WLS gene.
REFERENCE   2  (bases 1 to 2482)
  AUTHORS   Yu,C., Wang,C., Zhou,W., Zhang,A., Jia,Z., Zheng,B. and Ding,G.
  TITLE     Compound heterozygous variants in WLS gene causes Zaki syndrome
  JOURNAL   Clin Genet 104 (2), 226-229 (2023)
   PUBMED   37005218
  REMARK    GeneRIF: Compound heterozygous variants in WLS gene causes Zaki
            syndrome.
REFERENCE   3  (bases 1 to 2482)
  AUTHORS   Kantaputra,P., Tripuwabhrut,K., Jatooratthawichot,P.,
            Adisornkanj,P., Hatsadaloi,A., Porntrakoolsaree,N., Kaewgaya,M.,
            Olsen,B., Tongsima,S., Ngamphiw,C. and Ketudat Cairns,J.R.
  TITLE     Mutations in the WLS are associated with dental anomalies, torus
            palatinus, and torus mandibularis
  JOURNAL   Eur J Orthod 45 (3), 317-323 (2023)
   PUBMED   36374649
  REMARK    GeneRIF: Mutations in the WLS are associated with dental anomalies,
            torus palatinus, and torus mandibularis.
REFERENCE   4  (bases 1 to 2482)
  AUTHORS   Wolf,L. and Boutros,M.
  TITLE     The role of Evi/Wntless in exporting Wnt proteins
  JOURNAL   Development 150 (3) (2023)
   PUBMED   36763105
  REMARK    GeneRIF: The role of Evi/Wntless in exporting Wnt proteins.
            Review article
REFERENCE   5  (bases 1 to 2482)
  AUTHORS   Xie,Y.K., Luo,H., Zhang,S.X., Chen,X.Y., Guo,R., Qiu,X.Y., Liu,S.,
            Wu,H., Chen,W.B., Zhen,X.H., Ma,Q., Tian,J.L., Li,S., Chen,X.,
            Han,Q., Duan,S., Shen,C., Yang,F. and Xu,Z.Z.
  TITLE     GPR177 in A-fiber sensory neurons drives diabetic neuropathic pain
            via WNT-mediated TRPV1 activation
  JOURNAL   Sci Transl Med 14 (639), eabh2557 (2022)
   PUBMED   35385340
  REMARK    GeneRIF: GPR177 in A-fiber sensory neurons drives diabetic
            neuropathic pain via WNT-mediated TRPV1 activation.
REFERENCE   6  (bases 1 to 2482)
  AUTHORS   Belenkaya,T.Y., Wu,Y., Tang,X., Zhou,B., Cheng,L., Sharma,Y.V.,
            Yan,D., Selva,E.M. and Lin,X.
  TITLE     The retromer complex influences Wnt secretion by recycling wntless
            from endosomes to the trans-Golgi network
  JOURNAL   Dev Cell 14 (1), 120-131 (2008)
   PUBMED   18160348
REFERENCE   7  (bases 1 to 2482)
  AUTHORS   Binnerts,M.E., Kim,K.A., Bright,J.M., Patel,S.M., Tran,K., Zhou,M.,
            Leung,J.M., Liu,Y., Lomas,W.E. 3rd, Dixon,M., Hazell,S.A.,
            Wagle,M., Nie,W.S., Tomasevic,N., Williams,J., Zhan,X., Levy,M.D.,
            Funk,W.D. and Abo,A.
  TITLE     R-Spondin1 regulates Wnt signaling by inhibiting internalization of
            LRP6
  JOURNAL   Proc Natl Acad Sci U S A 104 (37), 14700-14705 (2007)
   PUBMED   17804805
REFERENCE   8  (bases 1 to 2482)
  AUTHORS   Bartscherer,K., Pelte,N., Ingelfinger,D. and Boutros,M.
  TITLE     Secretion of Wnt ligands requires Evi, a conserved transmembrane
            protein
  JOURNAL   Cell 125 (3), 523-533 (2006)
   PUBMED   16678096
REFERENCE   9  (bases 1 to 2482)
  AUTHORS   Banziger,C., Soldini,D., Schutt,C., Zipperlen,P., Hausmann,G. and
            Basler,K.
  TITLE     Wntless, a conserved membrane protein dedicated to the secretion of
            Wnt proteins from signaling cells
  JOURNAL   Cell 125 (3), 509-522 (2006)
   PUBMED   16678095
REFERENCE   10 (bases 1 to 2482)
  AUTHORS   Matsuda,A., Suzuki,Y., Honda,G., Muramatsu,S., Matsuzaki,O.,
            Nagano,Y., Doi,T., Shimotohno,K., Harada,T., Nishida,E., Hayashi,H.
            and Sugano,S.
  TITLE     Large-scale identification and characterization of human genes that
            activate NF-kappaB and MAPK signaling pathways
  JOURNAL   Oncogene 22 (21), 3307-3318 (2003)
   PUBMED   12761501
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            DA753300.1, DB478499.1, BX648748.1, AL513284.12 and AW137622.1.
            
            Transcript Variant: This variant (3) differs in the 5' UTR and
            lacks an alternate in-frame exon in the 5' coding region, compared
            to variant 1, resulting in an isoform (3) that is shorter than
            isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BX648748.1, SRR14038195.1480134.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-147               DA753300.1         392-538
            148-233             DB478499.1         230-315
            234-1366            BX648748.1         232-1364
            1367-1367           AL513284.12        26045-26045         c
            1368-2103           BX648748.1         1366-2101
            2104-2401           AL513284.12        13567-13864         c
            2402-2405           AL513284.12        13563-13566         c
            2406-2482           AW137622.1         1-77                c
FEATURES             Location/Qualifiers
     source          1..2482
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1p31.3"
     gene            1..2482
                     /gene="WLS"
                     /gene_synonym="C1orf139; EVI; GPR177; mig-14; MRP; ZKS"
                     /note="Wnt ligand secretion mediator"
                     /db_xref="GeneID:79971"
                     /db_xref="HGNC:HGNC:30238"
                     /db_xref="MIM:611514"
     exon            1..353
                     /gene="WLS"
                     /gene_synonym="C1orf139; EVI; GPR177; mig-14; MRP; ZKS"
                     /inference="alignment:Splign:2.1.0"
     CDS             248..1600
                     /gene="WLS"
                     /gene_synonym="C1orf139; EVI; GPR177; mig-14; MRP; ZKS"
                     /note="isoform 3 is encoded by transcript variant 3; G
                     protein-coupled receptor 177; integral membrane protein
                     GPR177; putative NFkB activating protein 373; protein
                     evenness interrupted homolog; putative
                     NF-kappa-B-activating protein 373; wntless Wnt ligand
                     secretion mediator"
                     /codon_start=1
                     /product="protein wntless homolog isoform 3"
                     /protein_id="NP_001180263.1"
                     /db_xref="CCDS:CCDS53331.1"
                     /db_xref="GeneID:79971"
                     /db_xref="HGNC:HGNC:30238"
                     /db_xref="MIM:611514"
                     /translation="MAGAIIENMSTKKLCIVGGILLVFQIIAFLVGGLIGENAEVSMD
                     VSLAYRDDAFAEWTEMAHERVPRKLKCTFTSPKTPEHEGRYYECDVLPFMEIGSVAHK
                     FYLLNIRLPVNEKKKINVGIGEIKDIRLVGIHQNGGFTKVWFAMKTFLTPSIFIIMVW
                     YWRRITMMSRPPVLLEKVIFALGISMTFINIPVEWFSIGFDWTWMLLFGDIRQGIFYA
                     MLLSFWIIFCGEHMMDQHERNHIAGYWKQVGPIAVGSFCLFIFDMCERGVQLTNPFYS
                     IWTTDIGTELAMAFIIVAGICLCLYFLFLCFMVFQVFRNISGKQSSLPAMSKVRRLHY
                     EGLIFRFKFLMLITLACAAMTVIFFIVSQVTEGHWKWGGVTVQVNSAFFTGIYGMWNL
                     YVFALMFLYAPSHKNYGEDQSNGDLGVHSGEELQLTTTITHVDGPTEIYKLTRKEAQE
                     "
     exon            354..478
                     /gene="WLS"
                     /gene_synonym="C1orf139; EVI; GPR177; mig-14; MRP; ZKS"
                     /inference="alignment:Splign:2.1.0"
     exon            479..640
                     /gene="WLS"
                     /gene_synonym="C1orf139; EVI; GPR177; mig-14; MRP; ZKS"
                     /inference="alignment:Splign:2.1.0"
     exon            641..777
                     /gene="WLS"
                     /gene_synonym="C1orf139; EVI; GPR177; mig-14; MRP; ZKS"
                     /inference="alignment:Splign:2.1.0"
     exon            778..946
                     /gene="WLS"
                     /gene_synonym="C1orf139; EVI; GPR177; mig-14; MRP; ZKS"
                     /inference="alignment:Splign:2.1.0"
     exon            947..1044
                     /gene="WLS"
                     /gene_synonym="C1orf139; EVI; GPR177; mig-14; MRP; ZKS"
                     /inference="alignment:Splign:2.1.0"
     exon            1045..1108
                     /gene="WLS"
                     /gene_synonym="C1orf139; EVI; GPR177; mig-14; MRP; ZKS"
                     /inference="alignment:Splign:2.1.0"
     exon            1109..1252
                     /gene="WLS"
                     /gene_synonym="C1orf139; EVI; GPR177; mig-14; MRP; ZKS"
                     /inference="alignment:Splign:2.1.0"
     exon            1253..1336
                     /gene="WLS"
                     /gene_synonym="C1orf139; EVI; GPR177; mig-14; MRP; ZKS"
                     /inference="alignment:Splign:2.1.0"
     exon            1337..1490
                     /gene="WLS"
                     /gene_synonym="C1orf139; EVI; GPR177; mig-14; MRP; ZKS"
                     /inference="alignment:Splign:2.1.0"
     exon            1491..2468
                     /gene="WLS"
                     /gene_synonym="C1orf139; EVI; GPR177; mig-14; MRP; ZKS"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2300..2305
                     /regulatory_class="polyA_signal_sequence"
                     /gene="WLS"
                     /gene_synonym="C1orf139; EVI; GPR177; mig-14; MRP; ZKS"
                     /note="hexamer: ATTAAA"
     polyA_site      2334
                     /gene="WLS"
                     /gene_synonym="C1orf139; EVI; GPR177; mig-14; MRP; ZKS"
                     /note="major polyA site"
     regulatory      2409..2414
                     /regulatory_class="polyA_signal_sequence"
                     /gene="WLS"
                     /gene_synonym="C1orf139; EVI; GPR177; mig-14; MRP; ZKS"
                     /note="hexamer: AATAAA"
     polyA_site      2430
                     /gene="WLS"
                     /gene_synonym="C1orf139; EVI; GPR177; mig-14; MRP; ZKS"
     polyA_site      2466
                     /gene="WLS"
                     /gene_synonym="C1orf139; EVI; GPR177; mig-14; MRP; ZKS"
ORIGIN      
        1 cgtccacagg cggcggagcg gccacaatca cagctccggg cattggggga acccgagccg
       61 gctgcgccgg gggaatccgt gcgggcgcct tccgtcccgg tcccatcctc gccgcgctcc
      121 agcacctctg aagttttgca gcgcccagaa aggaggcgag gaaggaggga gtgtgtgaga
      181 ggagggagca aaaagctcac cctaaaacat ttatttcaag gagaaaagaa aaaggggggg
      241 cgcaaaaatg gctggggcaa ttatagaaaa catgagcacc aagaagctgt gcattgttgg
      301 tgggattctg ctcgtgttcc aaatcatcgc ctttctggtg ggaggcttga ttggagaaaa
      361 tgcagaagtc tccatggacg tttccctggc ttaccgtgat gacgcgtttg ctgagtggac
      421 tgaaatggcc catgaaagag taccacggaa actcaaatgc accttcacat ctcccaagac
      481 tccagagcat gagggccgtt actatgaatg tgatgtcctt cctttcatgg aaattgggtc
      541 tgtggcccat aagttttacc ttttaaacat ccggctgcct gtgaatgaga agaagaaaat
      601 caatgtggga attggggaga taaaggatat ccggttggtg gggatccacc aaaatggagg
      661 cttcaccaag gtgtggtttg ccatgaagac cttccttacg cccagcatct tcatcattat
      721 ggtgtggtat tggaggagga tcaccatgat gtcccgaccc ccagtgcttc tggaaaaagt
      781 catctttgcc cttgggattt ccatgacctt tatcaatatc ccagtggaat ggttttccat
      841 cgggtttgac tggacctgga tgctgctgtt tggtgacatc cgacagggca tcttctatgc
      901 gatgcttctg tccttctgga tcatcttctg tggcgagcac atgatggatc agcacgagcg
      961 gaaccacatc gcagggtatt ggaagcaagt cggacccatt gccgttggct ccttctgcct
     1021 cttcatattt gacatgtgtg agagaggggt acaactcacg aatcccttct acagtatctg
     1081 gactacagac attggaacag agctggccat ggccttcatc atcgtggctg gaatctgcct
     1141 ctgcctctac ttcctgtttc tatgcttcat ggtatttcag gtgtttcgga acatcagtgg
     1201 gaagcagtcc agcctgccag ctatgagcaa agtccggcgg ctacactatg aggggctaat
     1261 ttttaggttc aagttcctca tgcttatcac cttggcctgc gctgccatga ctgtcatctt
     1321 cttcatcgtt agtcaggtaa cggaaggcca ttggaaatgg ggcggcgtca cagtccaagt
     1381 gaacagtgcc tttttcacag gcatctatgg gatgtggaat ctgtatgtct ttgctctgat
     1441 gttcttgtat gcaccatccc ataaaaacta tggagaagac cagtccaatg gcgatctggg
     1501 tgtccatagt ggggaagaac tccagctcac caccactatc acccatgtgg acggacccac
     1561 tgagatctac aagttgaccc gcaaggaggc ccaggagtag gaggctgcag cgcccggctg
     1621 ggacggtctc tccatacccc agcccctcta actagagtgg ggagcatgcc agagagagct
     1681 caatgtacaa atgaatgcct catggctctt agctgtggtt tcttggacca gcggcatgga
     1741 catttgtcag tttgccttct gacggtagct tttggaggaa gattcctgca gccactaatg
     1801 cattgtgtat gataacaaaa actctggtat gacacatttt ctgtgatcat tgttaattag
     1861 tgacatagta acatctgtag cagctggtta gtaaacctca tgtgggggtg gggtgggggt
     1921 gtattccttg ggggatggtt tgggccgaat ggggagtgga atatttgaca tttttcctgt
     1981 tttaaattct aggatagatt ttaacatcct ttgcggtccc agtccaaggt aggctggtgt
     2041 catagtcttc tcactcctaa tccatgacca ctgttttttt cctatttata tcaccaggta
     2101 gcccactgag ttaatattta agttgtcaat agataagtgt ccctgttttg tggcataata
     2161 taactgaatt tcatgagaag atttattcca ccaggggtat ttcagctttg aaaccaaatc
     2221 tgtgtatcta atactaacca atctgttgga tgtgggtttt aaaaaatgtt tgctaaacta
     2281 cccaagtaag atttactgta ttaaatggcc ttcgggtctg aaaagctttt ttaacttctt
     2341 gcttaaaatg cgttttattt tgataagata cttcaaatag cctccaaaag tgtagatcca
     2401 atcatttaaa taaacctgta tgtatatgca tatgtgtaca tgcatatccc ctcctgctaa
     2461 aactcataaa aaaaaaaaaa aa
//
