LOCUS       NM_001002292            2029 bp    mRNA    linear   PRI 07-APR-2024
DEFINITION  Homo sapiens Wnt ligand secretion mediator (WLS), transcript
            variant 2, mRNA.
ACCESSION   NM_001002292
VERSION     NM_001002292.4
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Abdel-Salam,G.M.H., Afifi,H.H., Abdel-Hamid,M.S., Ahmed,N.E.B.,
            Taher,M.B., El-Kamah,G., Thiele,H., Nurnberg,P.N. and Bolz,H.J.
  TITLE     Expanding the phenotypic spectrum and clinical severity associated
            with WLS gene
  JOURNAL   J Hum Genet 68 (9), 607-613 (2023)
   PUBMED   37106064
  REMARK    GeneRIF: Expanding the phenotypic spectrum and clinical severity
            associated with WLS gene.
REFERENCE   2  (bases 1 to 2029)
  AUTHORS   Yu,C., Wang,C., Zhou,W., Zhang,A., Jia,Z., Zheng,B. and Ding,G.
  TITLE     Compound heterozygous variants in WLS gene causes Zaki syndrome
  JOURNAL   Clin Genet 104 (2), 226-229 (2023)
   PUBMED   37005218
  REMARK    GeneRIF: Compound heterozygous variants in WLS gene causes Zaki
            syndrome.
REFERENCE   3  (bases 1 to 2029)
  AUTHORS   Kantaputra,P., Tripuwabhrut,K., Jatooratthawichot,P.,
            Adisornkanj,P., Hatsadaloi,A., Porntrakoolsaree,N., Kaewgaya,M.,
            Olsen,B., Tongsima,S., Ngamphiw,C. and Ketudat Cairns,J.R.
  TITLE     Mutations in the WLS are associated with dental anomalies, torus
            palatinus, and torus mandibularis
  JOURNAL   Eur J Orthod 45 (3), 317-323 (2023)
   PUBMED   36374649
  REMARK    GeneRIF: Mutations in the WLS are associated with dental anomalies,
            torus palatinus, and torus mandibularis.
REFERENCE   4  (bases 1 to 2029)
  AUTHORS   Wolf,L. and Boutros,M.
  TITLE     The role of Evi/Wntless in exporting Wnt proteins
  JOURNAL   Development 150 (3) (2023)
   PUBMED   36763105
  REMARK    GeneRIF: The role of Evi/Wntless in exporting Wnt proteins.
            Review article
REFERENCE   5  (bases 1 to 2029)
  AUTHORS   Xie,Y.K., Luo,H., Zhang,S.X., Chen,X.Y., Guo,R., Qiu,X.Y., Liu,S.,
            Wu,H., Chen,W.B., Zhen,X.H., Ma,Q., Tian,J.L., Li,S., Chen,X.,
            Han,Q., Duan,S., Shen,C., Yang,F. and Xu,Z.Z.
  TITLE     GPR177 in A-fiber sensory neurons drives diabetic neuropathic pain
            via WNT-mediated TRPV1 activation
  JOURNAL   Sci Transl Med 14 (639), eabh2557 (2022)
   PUBMED   35385340
  REMARK    GeneRIF: GPR177 in A-fiber sensory neurons drives diabetic
            neuropathic pain via WNT-mediated TRPV1 activation.
REFERENCE   6  (bases 1 to 2029)
  AUTHORS   Belenkaya,T.Y., Wu,Y., Tang,X., Zhou,B., Cheng,L., Sharma,Y.V.,
            Yan,D., Selva,E.M. and Lin,X.
  TITLE     The retromer complex influences Wnt secretion by recycling wntless
            from endosomes to the trans-Golgi network
  JOURNAL   Dev Cell 14 (1), 120-131 (2008)
   PUBMED   18160348
REFERENCE   7  (bases 1 to 2029)
  AUTHORS   Binnerts,M.E., Kim,K.A., Bright,J.M., Patel,S.M., Tran,K., Zhou,M.,
            Leung,J.M., Liu,Y., Lomas,W.E. 3rd, Dixon,M., Hazell,S.A.,
            Wagle,M., Nie,W.S., Tomasevic,N., Williams,J., Zhan,X., Levy,M.D.,
            Funk,W.D. and Abo,A.
  TITLE     R-Spondin1 regulates Wnt signaling by inhibiting internalization of
            LRP6
  JOURNAL   Proc Natl Acad Sci U S A 104 (37), 14700-14705 (2007)
   PUBMED   17804805
REFERENCE   8  (bases 1 to 2029)
  AUTHORS   Bartscherer,K., Pelte,N., Ingelfinger,D. and Boutros,M.
  TITLE     Secretion of Wnt ligands requires Evi, a conserved transmembrane
            protein
  JOURNAL   Cell 125 (3), 523-533 (2006)
   PUBMED   16678096
REFERENCE   9  (bases 1 to 2029)
  AUTHORS   Banziger,C., Soldini,D., Schutt,C., Zipperlen,P., Hausmann,G. and
            Basler,K.
  TITLE     Wntless, a conserved membrane protein dedicated to the secretion of
            Wnt proteins from signaling cells
  JOURNAL   Cell 125 (3), 509-522 (2006)
   PUBMED   16678095
REFERENCE   10 (bases 1 to 2029)
  AUTHORS   Matsuda,A., Suzuki,Y., Honda,G., Muramatsu,S., Matsuzaki,O.,
            Nagano,Y., Doi,T., Shimotohno,K., Harada,T., Nishida,E., Hayashi,H.
            and Sugano,S.
  TITLE     Large-scale identification and characterization of human genes that
            activate NF-kappaB and MAPK signaling pathways
  JOURNAL   Oncogene 22 (21), 3307-3318 (2003)
   PUBMED   12761501
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            DA753300.1, BX538320.1, AL513284.12 and CA438784.1.
            
            On Aug 13, 2020 this sequence version replaced NM_001002292.3.
            
            Transcript Variant: This variant (2) uses an alternate in-frame
            splice site in the 5' coding region, and differs in the 5' UTR, 3'
            coding region and 3' UTR, compared to variant 1. The resulting
            isoform (2) has a distinct C-terminus and is longer than isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BX538320.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-76                DA753300.1         415-490
            77-624              BX538320.1         100-647
            625-650             AL513284.12        47337-47362         c
            651-2015            BX538320.1         674-2038
            2016-2029           CA438784.1         18-31               c
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1p31.3"
     gene            1..2029
                     /gene="WLS"
                     /gene_synonym="C1orf139; EVI; GPR177; mig-14; MRP; ZKS"
                     /note="Wnt ligand secretion mediator"
                     /db_xref="GeneID:79971"
                     /db_xref="HGNC:HGNC:30238"
                     /db_xref="MIM:611514"
     exon            1..330
                     /gene="WLS"
                     /gene_synonym="C1orf139; EVI; GPR177; mig-14; MRP; ZKS"
                     /inference="alignment:Splign:2.1.0"
     CDS             225..1856
                     /gene="WLS"
                     /gene_synonym="C1orf139; EVI; GPR177; mig-14; MRP; ZKS"
                     /note="isoform 2 is encoded by transcript variant 2; G
                     protein-coupled receptor 177; integral membrane protein
                     GPR177; putative NFkB activating protein 373; protein
                     evenness interrupted homolog; putative
                     NF-kappa-B-activating protein 373; wntless Wnt ligand
                     secretion mediator"
                     /codon_start=1
                     /product="protein wntless homolog isoform 2"
                     /protein_id="NP_001002292.3"
                     /db_xref="CCDS:CCDS30750.1"
                     /db_xref="GeneID:79971"
                     /db_xref="HGNC:HGNC:30238"
                     /db_xref="MIM:611514"
                     /translation="MAGAIIENMSTKKLCIVGGILLVFQIIAFLVGGLIGPTTAVSYM
                     SVKCVDARKNHHKTKWFVPWGPNHCDKIRDIEEAIPREIEANDIVFSVHIPLPHMEMS
                     PWFQFMLFILQLDIAFKLNNQIRENAEVSMDVSLAYRDDAFAEWTEMAHERVPRKLKC
                     TFTSPKTPEHEGRYYECDVLPFMEIGSVAHKFYLLNIRLPVNEKKKINVGIGEIKDIR
                     LVGIHQNGGFTKVWFAMKTFLTPSIFIIMVWYWRRITMMSRPPVLLEKVIFALGISMT
                     FINIPVEWFSIGFDWTWMLLFGDIRQGIFYAMLLSFWIIFCGEHMMDQHERNHIAGYW
                     KQVGPIAVGSFCLFIFDMCERGVQLTNPFYSIWTTDIGTELAMAFIIVAGICLCLYFL
                     FLCFMVFQVFRNISGKQSSLPAMSKVRRLHYEGLIFRFKFLMLITLACAAMTVIFFIV
                     SQVTEGHWKWGGVTVQVNSAFFTGIYGMWNLYVFALMFLYAPSHKNYGEDQSNGMQLP
                     CKSREDCALFVSELYQELFSASKYSFINDNAASGI"
     exon            331..597
                     /gene="WLS"
                     /gene_synonym="C1orf139; EVI; GPR177; mig-14; MRP; ZKS"
                     /inference="alignment:Splign:2.1.0"
     exon            598..722
                     /gene="WLS"
                     /gene_synonym="C1orf139; EVI; GPR177; mig-14; MRP; ZKS"
                     /inference="alignment:Splign:2.1.0"
     exon            723..884
                     /gene="WLS"
                     /gene_synonym="C1orf139; EVI; GPR177; mig-14; MRP; ZKS"
                     /inference="alignment:Splign:2.1.0"
     exon            885..1021
                     /gene="WLS"
                     /gene_synonym="C1orf139; EVI; GPR177; mig-14; MRP; ZKS"
                     /inference="alignment:Splign:2.1.0"
     exon            1022..1190
                     /gene="WLS"
                     /gene_synonym="C1orf139; EVI; GPR177; mig-14; MRP; ZKS"
                     /inference="alignment:Splign:2.1.0"
     exon            1191..1288
                     /gene="WLS"
                     /gene_synonym="C1orf139; EVI; GPR177; mig-14; MRP; ZKS"
                     /inference="alignment:Splign:2.1.0"
     exon            1289..1352
                     /gene="WLS"
                     /gene_synonym="C1orf139; EVI; GPR177; mig-14; MRP; ZKS"
                     /inference="alignment:Splign:2.1.0"
     exon            1353..1496
                     /gene="WLS"
                     /gene_synonym="C1orf139; EVI; GPR177; mig-14; MRP; ZKS"
                     /inference="alignment:Splign:2.1.0"
     exon            1497..1580
                     /gene="WLS"
                     /gene_synonym="C1orf139; EVI; GPR177; mig-14; MRP; ZKS"
                     /inference="alignment:Splign:2.1.0"
     exon            1581..1734
                     /gene="WLS"
                     /gene_synonym="C1orf139; EVI; GPR177; mig-14; MRP; ZKS"
                     /inference="alignment:Splign:2.1.0"
     exon            1735..2029
                     /gene="WLS"
                     /gene_synonym="C1orf139; EVI; GPR177; mig-14; MRP; ZKS"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1994..1999
                     /regulatory_class="polyA_signal_sequence"
                     /gene="WLS"
                     /gene_synonym="C1orf139; EVI; GPR177; mig-14; MRP; ZKS"
                     /note="hexamer: AATAAA"
     polyA_site      2029
                     /gene="WLS"
                     /gene_synonym="C1orf139; EVI; GPR177; mig-14; MRP; ZKS"
                     /note="major polyA site"
ORIGIN      
        1 acaatcacag ctccgggcat tgggggaacc cgagccggct gcgccggggg aatccgtgcg
       61 ggcgccttcc gtcccggtcc catcctcgcc gcgctccagc acctctgaag ttttgcagcg
      121 cccagaaagg aggcgaggaa ggagggagtg tgtgagagga gggagcaaaa agctcaccct
      181 aaaacattta tttcaaggag aaaagaaaaa gggggggcgc aaaaatggct ggggcaatta
      241 tagaaaacat gagcaccaag aagctgtgca ttgttggtgg gattctgctc gtgttccaaa
      301 tcatcgcctt tctggtggga ggcttgattg ggcccacaac ggcagtgtcc tacatgtcgg
      361 tgaaatgtgt ggatgcccgt aagaaccatc acaagacaaa atggttcgtg ccttggggac
      421 ccaatcattg tgacaagatc cgagacattg aagaggcaat tccaagggaa attgaagcca
      481 atgacatcgt gttttctgtt cacattcccc tcccccacat ggagatgagt ccttggttcc
      541 aattcatgct gtttatcctg cagctggaca ttgccttcaa gctaaacaac caaatcagag
      601 aaaatgcaga agtctccatg gacgtttccc tggcttaccg tgatgacgcg tttgctgagt
      661 ggactgaaat ggcccatgaa agagtaccac ggaaactcaa atgcaccttc acatctccca
      721 agactccaga gcatgagggc cgttactatg aatgtgatgt ccttcctttc atggaaattg
      781 ggtctgtggc ccataagttt taccttttaa acatccggct gcctgtgaat gagaagaaga
      841 aaatcaatgt gggaattggg gagataaagg atatccggtt ggtggggatc caccaaaatg
      901 gaggcttcac caaggtgtgg tttgccatga agaccttcct tacgcccagc atcttcatca
      961 ttatggtgtg gtattggagg aggatcacca tgatgtcccg acccccagtg cttctggaaa
     1021 aagtcatctt tgcccttggg atttccatga cctttatcaa tatcccagtg gaatggtttt
     1081 ccatcgggtt tgactggacc tggatgctgc tgtttggtga catccgacag ggcatcttct
     1141 atgcgatgct tctgtccttc tggatcatct tctgtggcga gcacatgatg gatcagcacg
     1201 agcggaacca catcgcaggg tattggaagc aagtcggacc cattgccgtt ggctccttct
     1261 gcctcttcat atttgacatg tgtgagagag gggtacaact cacgaatccc ttctacagta
     1321 tctggactac agacattgga acagagctgg ccatggcctt catcatcgtg gctggaatct
     1381 gcctctgcct ctacttcctg tttctatgct tcatggtatt tcaggtgttt cggaacatca
     1441 gtgggaagca gtccagcctg ccagctatga gcaaagtccg gcggctacac tatgaggggc
     1501 taatttttag gttcaagttc ctcatgctta tcaccttggc ctgcgctgcc atgactgtca
     1561 tcttcttcat cgttagtcag gtaacggaag gccattggaa atggggcggc gtcacagtcc
     1621 aagtgaacag tgcctttttc acaggcatct atgggatgtg gaatctgtat gtctttgctc
     1681 tgatgttctt gtatgcacca tcccataaaa actatggaga agaccagtcc aatggaatgc
     1741 aactcccatg taaatcgagg gaagattgtg ctttgtttgt ttcggaactt tatcaagaat
     1801 tgttcagcgc ttcgaaatat tccttcatca atgacaacgc agcttctggt atttgagtca
     1861 acaaggcaac acatgtttat cagctttgca tttgcagttg tcacagtcac attgattgta
     1921 cttgtatacg cacacaaata cactcattta gcctttatct caaaatgtta aatataagga
     1981 aaaaagcgtc aacaataaat attctttgag tattgtctta cttctctta
//
