LOCUS       NM_015610               4445 bp    mRNA    linear   PRI 24-SEP-2024
DEFINITION  Homo sapiens WD repeat domain, phosphoinositide interacting 2
            (WIPI2), transcript variant 1, mRNA.
ACCESSION   NM_015610
VERSION     NM_015610.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4445)
  AUTHORS   Gubas,A., Attridge,E., Jefferies,H.B., Nishimura,T., Razi,M.,
            Kunzelmann,S., Gilad,Y., Mercer,T.J., Wilson,M.M., Kimchi,A. and
            Tooze,S.A.
  TITLE     WIPI2b recruitment to phagophores and ATG16L1 binding are regulated
            by ULK1 phosphorylation
  JOURNAL   EMBO Rep 25 (9), 3789-3811 (2024)
   PUBMED   39152217
  REMARK    GeneRIF: WIPI2b recruitment to phagophores and ATG16L1 binding are
            regulated by ULK1 phosphorylation.
REFERENCE   2  (bases 1 to 4445)
  AUTHORS   Wan,W., Qian,C., Wang,Q., Li,J., Zhang,H., Wang,L., Pu,M.,
            Huang,Y., He,Z., Zhou,T., Shen,H.M. and Liu,W.
  TITLE     STING directly recruits WIPI2 for autophagosome formation during
            STING-induced autophagy
  JOURNAL   EMBO J 42 (8), e112387 (2023)
   PUBMED   36872914
  REMARK    GeneRIF: STING directly recruits WIPI2 for autophagosome formation
            during STING-induced autophagy.
REFERENCE   3  (bases 1 to 4445)
  AUTHORS   Strong,L.M., Chang,C., Riley,J.F., Boecker,C.A., Flower,T.G.,
            Buffalo,C.Z., Ren,X., Stavoe,A.K., Holzbaur,E.L. and Hurley,J.H.
  TITLE     Structural basis for membrane recruitment of ATG16L1 by WIPI2 in
            autophagy
  JOURNAL   Elife 10, e70372 (2021)
   PUBMED   34505572
  REMARK    GeneRIF: Structural basis for membrane recruitment of ATG16L1 by
            WIPI2 in autophagy.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 4445)
  AUTHORS   Kojima,W., Yamano,K., Kosako,H., Imai,K., Kikuchi,R., Tanaka,K. and
            Matsuda,N.
  TITLE     Mammalian BCAS3 and C16orf70 associate with the phagophore assembly
            site in response to selective and non-selective autophagy
  JOURNAL   Autophagy 17 (8), 2011-2036 (2021)
   PUBMED   33499712
REFERENCE   5  (bases 1 to 4445)
  AUTHORS   Fracchiolla,D., Chang,C., Hurley,J.H. and Martens,S.
  TITLE     A PI3K-WIPI2 positive feedback loop allosterically activates LC3
            lipidation in autophagy
  JOURNAL   J Cell Biol 219 (7) (2020)
   PUBMED   32437499
  REMARK    GeneRIF: A PI3K-WIPI2 positive feedback loop allosterically
            activates LC3 lipidation in autophagy.
REFERENCE   6  (bases 1 to 4445)
  AUTHORS   Proikas-Cezanne,T. and Robenek,H.
  TITLE     Freeze-fracture replica immunolabelling reveals human WIPI-1 and
            WIPI-2 as membrane proteins of autophagosomes
  JOURNAL   J Cell Mol Med 15 (9), 2007-2010 (2011)
   PUBMED   21564513
  REMARK    GeneRIF: Freeze-fracture replica immunolabelling reveals WD-repeat
            protein interacting with phosphoinositides 1 and 2 (WIPI-1 and
            WIPI-2) as membrane components of autophagosomes and the plasma
            membrane (PM).
REFERENCE   7  (bases 1 to 4445)
  AUTHORS   Lee,O.H., Kim,H., He,Q., Baek,H.J., Yang,D., Chen,L.Y., Liang,J.,
            Chae,H.K., Safari,A., Liu,D. and Songyang,Z.
  TITLE     Genome-wide YFP fluorescence complementation screen identifies new
            regulators for telomere signaling in human cells
  JOURNAL   Mol Cell Proteomics 10 (2), M110.001628 (2011)
   PUBMED   21044950
REFERENCE   8  (bases 1 to 4445)
  AUTHORS   Polson,H.E., de Lartigue,J., Rigden,D.J., Reedijk,M., Urbe,S.,
            Clague,M.J. and Tooze,S.A.
  TITLE     Mammalian Atg18 (WIPI2) localizes to omegasome-anchored phagophores
            and positively regulates LC3 lipidation
  JOURNAL   Autophagy 6 (4), 506-522 (2010)
   PUBMED   20505359
  REMARK    GeneRIF: A role for WIPI2 in the progression of omegasomes into
            autophagosomes, is reported.
            GeneRIF: WIPI2 is a phosphatidylinsitol-3-phosphate binding protein
            required for starvation induced autophagy.
REFERENCE   9  (bases 1 to 4445)
  AUTHORS   Proikas-Cezanne,T., Waddell,S., Gaugel,A., Frickey,T., Lupas,A. and
            Nordheim,A.
  TITLE     WIPI-1alpha (WIPI49), a member of the novel 7-bladed WIPI protein
            family, is aberrantly expressed in human cancer and is linked to
            starvation-induced autophagy
  JOURNAL   Oncogene 23 (58), 9314-9325 (2004)
   PUBMED   15602573
REFERENCE   10 (bases 1 to 4445)
  AUTHORS   Simpson,J.C., Wellenreuther,R., Poustka,A., Pepperkok,R. and
            Wiemann,S.
  TITLE     Systematic subcellular localization of novel proteins identified by
            large-scale cDNA sequencing
  JOURNAL   EMBO Rep 1 (3), 287-292 (2000)
   PUBMED   11256614
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL080155.1 and AC093376.5.
            
            On Nov 22, 2018 this sequence version replaced NM_015610.3.
            
            Summary: WD40 repeat proteins are key components of many essential
            biologic functions. They regulate the assembly of multiprotein
            complexes by presenting a beta-propeller platform for simultaneous
            and reversible protein-protein interactions. Members of the WIPI
            subfamily of WD40 repeat proteins, such as WIPI2, have a 7-bladed
            propeller structure and contain a conserved motif for interaction
            with phospholipids (Proikas-Cezanne et al., 2004 [PubMed
            15602573]).[supplied by OMIM, Mar 2008].
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (a).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AL080155.1, AK023041.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000288828.9/ ENSP00000288828.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-40                AL080155.1         31-70
            41-261              AC093376.5         12609-12829
            262-315             AC093376.5         15454-15507
            316-398             AC093376.5         21912-21994
            399-568             AC093376.5         36871-37040
            569-665             AC093376.5         38899-38995
            666-763             AC093376.5         39426-39523
            764-856             AC093376.5         40258-40350
            857-927             AC093376.5         44938-45008
            928-1035            AC093376.5         48159-48266
            1036-1200           AC093376.5         49516-49680
            1201-1308           AC093376.5         50440-50547
            1309-1439           AC093376.5         51944-52074
            1440-4445           AC093376.5         53171-56176
FEATURES             Location/Qualifiers
     source          1..4445
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="7"
                     /map="7p22.1"
     gene            1..4445
                     /gene="WIPI2"
                     /gene_synonym="ATG18B; Atg21; CGI-50; IDDSSA; WIPI-2"
                     /note="WD repeat domain, phosphoinositide interacting 2"
                     /db_xref="GeneID:26100"
                     /db_xref="HGNC:HGNC:32225"
                     /db_xref="MIM:609225"
     exon            1..261
                     /gene="WIPI2"
                     /gene_synonym="ATG18B; Atg21; CGI-50; IDDSSA; WIPI-2"
                     /inference="alignment:Splign:2.1.0"
     CDS             188..1552
                     /gene="WIPI2"
                     /gene_synonym="ATG18B; Atg21; CGI-50; IDDSSA; WIPI-2"
                     /note="isoform a is encoded by transcript variant 1;
                     WIPI49-like protein 2; WD40 repeat protein interacting
                     with phosphoinositides 2; WD repeat domain
                     phosphoinositide-interacting protein 2"
                     /codon_start=1
                     /product="WD repeat domain phosphoinositide-interacting
                     protein 2 isoform a"
                     /protein_id="NP_056425.1"
                     /db_xref="CCDS:CCDS5339.1"
                     /db_xref="GeneID:26100"
                     /db_xref="HGNC:HGNC:32225"
                     /db_xref="MIM:609225"
                     /translation="MNLASQSGEAGAGQLLFANFNQDNTEVKGASRAAGLGRRAVVWS
                     LAVGSKSGYKFFSLSSVDKLEQIYECTDTEDVCIVERLFSSSLVAIVSLKAPRKLKVC
                     HFKKGTEICNYSYSNTILAVKLNRQRLIVCLEESLYIHNIRDMKVLHTIRETPPNPAG
                     LCALSINNDNCYLAYPGSATIGEVQVFDTINLRAANMIPAHDSPLAALAFDASGTKLA
                     TASEKGTVIRVFSIPEGQKLFEFRRGVKRCVSICSLAFSMDGMFLSASSNTETVHIFK
                     LETVKEKPPEEPTTWTGYFGKVLMASTSYLPSQVTEMFNQGRAFATVRLPFCGHKNIC
                     SLATIQKIPRLLVGAADGYLYMYNLDPQEGGECALMKQHRLDGSLETTNEILDSASHD
                     CPLVTQTYGAAAGKGTYVPSSPTRLAYTDDLGAVGGACLEDEASALRLDEDSEHPPMI
                     LRTD"
     exon            262..315
                     /gene="WIPI2"
                     /gene_synonym="ATG18B; Atg21; CGI-50; IDDSSA; WIPI-2"
                     /inference="alignment:Splign:2.1.0"
     exon            316..398
                     /gene="WIPI2"
                     /gene_synonym="ATG18B; Atg21; CGI-50; IDDSSA; WIPI-2"
                     /inference="alignment:Splign:2.1.0"
     exon            399..568
                     /gene="WIPI2"
                     /gene_synonym="ATG18B; Atg21; CGI-50; IDDSSA; WIPI-2"
                     /inference="alignment:Splign:2.1.0"
     exon            569..665
                     /gene="WIPI2"
                     /gene_synonym="ATG18B; Atg21; CGI-50; IDDSSA; WIPI-2"
                     /inference="alignment:Splign:2.1.0"
     exon            666..763
                     /gene="WIPI2"
                     /gene_synonym="ATG18B; Atg21; CGI-50; IDDSSA; WIPI-2"
                     /inference="alignment:Splign:2.1.0"
     exon            764..856
                     /gene="WIPI2"
                     /gene_synonym="ATG18B; Atg21; CGI-50; IDDSSA; WIPI-2"
                     /inference="alignment:Splign:2.1.0"
     exon            857..927
                     /gene="WIPI2"
                     /gene_synonym="ATG18B; Atg21; CGI-50; IDDSSA; WIPI-2"
                     /inference="alignment:Splign:2.1.0"
     exon            928..1035
                     /gene="WIPI2"
                     /gene_synonym="ATG18B; Atg21; CGI-50; IDDSSA; WIPI-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1036..1200
                     /gene="WIPI2"
                     /gene_synonym="ATG18B; Atg21; CGI-50; IDDSSA; WIPI-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1201..1308
                     /gene="WIPI2"
                     /gene_synonym="ATG18B; Atg21; CGI-50; IDDSSA; WIPI-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1309..1439
                     /gene="WIPI2"
                     /gene_synonym="ATG18B; Atg21; CGI-50; IDDSSA; WIPI-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1440..4445
                     /gene="WIPI2"
                     /gene_synonym="ATG18B; Atg21; CGI-50; IDDSSA; WIPI-2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2109..2114
                     /regulatory_class="polyA_signal_sequence"
                     /gene="WIPI2"
                     /gene_synonym="ATG18B; Atg21; CGI-50; IDDSSA; WIPI-2"
                     /note="hexamer: ATTAAA"
     polyA_site      2130
                     /gene="WIPI2"
                     /gene_synonym="ATG18B; Atg21; CGI-50; IDDSSA; WIPI-2"
     regulatory      2304..2309
                     /regulatory_class="polyA_signal_sequence"
                     /gene="WIPI2"
                     /gene_synonym="ATG18B; Atg21; CGI-50; IDDSSA; WIPI-2"
                     /note="hexamer: ATTAAA"
     polyA_site      2329
                     /gene="WIPI2"
                     /gene_synonym="ATG18B; Atg21; CGI-50; IDDSSA; WIPI-2"
                     /note="major polyA site"
     regulatory      4407..4412
                     /regulatory_class="polyA_signal_sequence"
                     /gene="WIPI2"
                     /gene_synonym="ATG18B; Atg21; CGI-50; IDDSSA; WIPI-2"
                     /note="hexamer: AATAAA"
     regulatory      4421..4426
                     /regulatory_class="polyA_signal_sequence"
                     /gene="WIPI2"
                     /gene_synonym="ATG18B; Atg21; CGI-50; IDDSSA; WIPI-2"
                     /note="hexamer: CATAAA"
     polyA_site      4426
                     /gene="WIPI2"
                     /gene_synonym="ATG18B; Atg21; CGI-50; IDDSSA; WIPI-2"
     polyA_site      4445
                     /gene="WIPI2"
                     /gene_synonym="ATG18B; Atg21; CGI-50; IDDSSA; WIPI-2"
ORIGIN      
        1 gtaccgggtg ccccggctct ggagcataaa caagagcggg gacgggatga ggcggcggtt
       61 gatcccaggg tggcgagtgg cggcgaccga ggcggcgagc ggggcccggc gccgaccctg
      121 agtgcagcct gacccgccct cgcgcgcgcg ccctccccgg ccgggcccac tcgccgcgcg
      181 cccagccatg aacctggcga gccagagcgg ggaggccggc gccggccagc tgctcttcgc
      241 caacttcaac caggacaaca cagaagtgaa aggggcatca agagcagctg gtcttggccg
      301 tcgcgctgtt gtctggtccc tagctgttgg tagtaagtcc ggttataaat ttttctccct
      361 ttcttctgtg gataagctgg aacagatcta tgaatgcacc gatacggaag atgtgtgcat
      421 tgtagagaga ttgttctcca gcagcctagt ggccatcgtc agccttaaag caccaaggaa
      481 gctaaaggtt tgccacttta agaagggaac tgagatctgc aactacagct actccaacac
      541 gattctggct gtgaagctca acaggcagag gctgatagta tgcctggagg agtccctgta
      601 catccacaac attcgggaca tgaaggtgct gcatacgatc agggagacgc ctccaaaccc
      661 tgcaggcctg tgtgcgctgt caatcaacaa cgacaactgc tacttggcgt acccagggag
      721 cgcgaccatc ggagaggtgc aggtcttcga taccattaat ttgagagctg caaacatgat
      781 tccggctcac gacagtcctt tagcggcact ggcctttgac gcaagtggaa ctaaacttgc
      841 cacggcttcg gagaagggga ccgtgattag ggtattttcc attccagaag gacaaaaact
      901 ctttgagttt cggagaggag taaagaggtg cgtgagcatc tgctccctgg ccttcagcat
      961 ggacggcatg ttcctctccg cctccagcaa cactgagacc gtgcacatct tcaaactcga
     1021 gactgtgaaa gaaaaacccc cagaggagcc caccacctgg accgggtact tcgggaaagt
     1081 gctcatggcc tccaccagct acctgccttc ccaagtgaca gaaatgttca accagggcag
     1141 agccttcgcc acggtccgcc tgccattctg cggccacaaa aacatctgct cgctagccac
     1201 aattcagaag atcccgcggt tgttggtggg tgccgccgac gggtacctgt acatgtacaa
     1261 cctggacccc caggagggcg gcgagtgtgc cctgatgaag cagcaccggc tggacggcag
     1321 tctggaaacg accaatgaga tcttggactc tgcctctcac gactgcccct tagtcactca
     1381 gacatacggc gcagctgcag gaaaaggtac ttacgtgcct tcatccccaa cgagacttgc
     1441 ctacacagac gacctgggtg ctgtgggtgg cgcctgcctg gaggacgagg ccagcgccct
     1501 gcgcctggat gaggacagcg agcacccgcc catgattctt cggactgact gaacttgacc
     1561 tgtgacctct gacccgggga gcagagaaca ctggcttcac agaggacttt gtgcattgct
     1621 gctatgaact ttgacctgag tcgggggaga ggatggcaga gactttatta aaaaaaaaaa
     1681 aagattgtag tggtagtcta actccataac gctgaggaaa tacatcattt tcacttcagt
     1741 ggcttttaaa tcctgcttat gaattttagc tttttgtttg tttgttttct ctttttgcca
     1801 aaattaactg tttggtgaag cccgcaaaac ctcctcgctt tgcatgcatg aacgtgccaa
     1861 gccagcatag gggagctaga agccactttc cagccacctg ccgttgggtt ttttcatatc
     1921 tgtacataat gccgagtgcg taaggaaacc gtggcgtcgc gcacagtggg tctgcttgtc
     1981 aaggccagtt ctgcagtgac aggcccaggg gctgcccacc aggtgtgctg ggcagacttc
     2041 agctgggaca gaagtccgat ctccctaggg ccccacctgg accattttcc ctccgtttta
     2101 ttttgttaat taaattcttt ccaaattgga tcgctctggg atttcttcca tggtggactt
     2161 ttgtttctga tcttgttttc cctgtggata ttggaggaca gcgaggttct ttctgatact
     2221 aaaaaccttt ctttcaggca gcaaatgaac ttgaaaggtt gcctggactc gctggagcaa
     2281 aggaaagcga ttttgtttgt ataattaaat gatctgttct tctacttcac tcttcctgtt
     2341 gaaaaactgt gtgatttttt ttttttttta agtaaagttt gtagctcctc ccatgcccag
     2401 gaaagcacag aactcaagtg tggtggccgt ctgagctgtc ctttcgctgg ccccgctgtc
     2461 cgcaggggct tcctacctgt gtgagaggtc gtagcgggag acagcaacag agagtaggcg
     2521 gctgggccac gtccttcaca gggcgtcatg tgcctttcta ttttcatctt agaaaatttc
     2581 ctcagcaaac cgatgagaga ttgtggttgc aagcttcagt atttgcctcg cttcccttcc
     2641 cttttcatat ttacagaatt aaaacaacct caagtacctc agactctgca ttccaaacca
     2701 aggcacccag cagccaaagt gtcgagggca tttaagtggc attaatggca ggagagatgg
     2761 ttttagaatc tatggagtgg tggaagttac ggatagaagg gaaaaggcaa aaactattta
     2821 ccctgccttt gcaggctggg gtttttgaac cgaggaaggc tgggacgcct gtttccagat
     2881 ggttgtcatg gtcacgctgg gcgaagagct ggaggggagt tgtcccctca gctgagcggc
     2941 tgcggtgaaa tgccccagtg ttcctgggtt ggctttacgg caaactaaat gcaggggacg
     3001 ctggagtccg actcacctac accggcttcc tcccagccgc tggtgtgcgg cacacacaac
     3061 atctgcatta ggcagaggtg caagtgggct gattgaactt ttccttcaaa acctgcttgt
     3121 ctgtcctgga cctttgatga aatgggatcc cggtcacgca ggctgagaca gtggggaccg
     3181 ccgaggccag agtgggctat gcttgagcag ggatgagaag ggccgcggca gcacgcagcc
     3241 ttgacccacg cctgcgtctt gtggtgcaag gccagagggc tctctctaga acctgaccgt
     3301 gcactccatc tcctgggagc cacttttggc aagagtgagt gtggggggaa aaagtgtgca
     3361 caagagatac ggaaccctga gctagggttt cctgtcacca aagagtgagc tgcatttcca
     3421 gttctgcgtt aaagtatcgt ttgttctggt gcctgcctgt ggtggtgatt tggagactca
     3481 aatttccttt gccaggtctc ttttgtcttt ctttgggatt ggtagtatag aagatgctgg
     3541 gaattgtctt cctcgctcag cctcggcttc ccgcactgta agatgaggca gttgcagagg
     3601 aggcctcgga cgtcctgtga ccctcggccc acactccccc ttgggtggcc ctggcagggc
     3661 tggctaggtg cgttccctgc agaccacggg aagccctgtg cttgcctggg tcagggctaa
     3721 gctgtgcgcc ctagaggagg gaggaggact gcagattctt ggtcgagaag aatgaagagg
     3781 atttctgtgt ttgctgagca gccattgtgg agggttaccc gcctccactt tcccgttgcc
     3841 catttcactg ccaccagctc ctcttcctct gctcgaacct atgagtccca gcctcactgg
     3901 cggcctcaga catgctcagg agtgacgggg acagagggag gccgctgagt tgcccgttag
     3961 aactcttact gctgcgccag tgacccaggt ggagagggac cctgaaccaa acagaacgtg
     4021 tgctaatttt ccgaactcca aactgtacac tcatattcat ttttaaattg tatttttcca
     4081 aacttcaaaa aggacgatga gcgtggggga taggaaacaa aacctgtaaa cgttgtgaat
     4141 gggctcagtg gactctggga ccaaaccttc tgtaatctct aaaacaatgg gaccaagagc
     4201 tggatggaac ctggagtcaa aaagaactgc ttcagtcccc gctgtaccgc ctgcctagct
     4261 gtgggagcag gcagggcgcc tgggagcctg cgttttctgg accgttccat gggactcatc
     4321 cctacctcac agggctgttg tgaggtgttg tgtgactgcg tgtcctgcaa acgcccagct
     4381 cggtgcccag ccagcggtgg gcacccaata aacgctacaa cataaatgtg tcttctgcaa
     4441 ttaaa
//
