LOCUS       NP_056425                454 aa            linear   PRI 28-APR-2025
DEFINITION  WD repeat domain phosphoinositide-interacting protein 2 isoform a
            [Homo sapiens].
ACCESSION   NP_056425
VERSION     NP_056425.1
DBSOURCE    REFSEQ: accession NM_015610.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (residues 1 to 454)
  AUTHORS   Gubas,A., Attridge,E., Jefferies,H.B., Nishimura,T., Razi,M.,
            Kunzelmann,S., Gilad,Y., Mercer,T.J., Wilson,M.M., Kimchi,A. and
            Tooze,S.A.
  TITLE     WIPI2b recruitment to phagophores and ATG16L1 binding are regulated
            by ULK1 phosphorylation
  JOURNAL   EMBO Rep 25 (9), 3789-3811 (2024)
   PUBMED   39152217
  REMARK    GeneRIF: WIPI2b recruitment to phagophores and ATG16L1 binding are
            regulated by ULK1 phosphorylation.
REFERENCE   2  (residues 1 to 454)
  AUTHORS   Wan,W., Qian,C., Wang,Q., Li,J., Zhang,H., Wang,L., Pu,M.,
            Huang,Y., He,Z., Zhou,T., Shen,H.M. and Liu,W.
  TITLE     STING directly recruits WIPI2 for autophagosome formation during
            STING-induced autophagy
  JOURNAL   EMBO J 42 (8), e112387 (2023)
   PUBMED   36872914
  REMARK    GeneRIF: STING directly recruits WIPI2 for autophagosome formation
            during STING-induced autophagy.
REFERENCE   3  (residues 1 to 454)
  AUTHORS   Strong,L.M., Chang,C., Riley,J.F., Boecker,C.A., Flower,T.G.,
            Buffalo,C.Z., Ren,X., Stavoe,A.K., Holzbaur,E.L. and Hurley,J.H.
  TITLE     Structural basis for membrane recruitment of ATG16L1 by WIPI2 in
            autophagy
  JOURNAL   Elife 10, e70372 (2021)
   PUBMED   34505572
  REMARK    GeneRIF: Structural basis for membrane recruitment of ATG16L1 by
            WIPI2 in autophagy.
            Publication Status: Online-Only
REFERENCE   4  (residues 1 to 454)
  AUTHORS   Kojima,W., Yamano,K., Kosako,H., Imai,K., Kikuchi,R., Tanaka,K. and
            Matsuda,N.
  TITLE     Mammalian BCAS3 and C16orf70 associate with the phagophore assembly
            site in response to selective and non-selective autophagy
  JOURNAL   Autophagy 17 (8), 2011-2036 (2021)
   PUBMED   33499712
REFERENCE   5  (residues 1 to 454)
  AUTHORS   Fracchiolla,D., Chang,C., Hurley,J.H. and Martens,S.
  TITLE     A PI3K-WIPI2 positive feedback loop allosterically activates LC3
            lipidation in autophagy
  JOURNAL   J Cell Biol 219 (7) (2020)
   PUBMED   32437499
  REMARK    GeneRIF: A PI3K-WIPI2 positive feedback loop allosterically
            activates LC3 lipidation in autophagy.
REFERENCE   6  (residues 1 to 454)
  AUTHORS   Proikas-Cezanne,T. and Robenek,H.
  TITLE     Freeze-fracture replica immunolabelling reveals human WIPI-1 and
            WIPI-2 as membrane proteins of autophagosomes
  JOURNAL   J Cell Mol Med 15 (9), 2007-2010 (2011)
   PUBMED   21564513
  REMARK    GeneRIF: Freeze-fracture replica immunolabelling reveals WD-repeat
            protein interacting with phosphoinositides 1 and 2 (WIPI-1 and
            WIPI-2) as membrane components of autophagosomes and the plasma
            membrane (PM).
REFERENCE   7  (residues 1 to 454)
  AUTHORS   Lee,O.H., Kim,H., He,Q., Baek,H.J., Yang,D., Chen,L.Y., Liang,J.,
            Chae,H.K., Safari,A., Liu,D. and Songyang,Z.
  TITLE     Genome-wide YFP fluorescence complementation screen identifies new
            regulators for telomere signaling in human cells
  JOURNAL   Mol Cell Proteomics 10 (2), M110.001628 (2011)
   PUBMED   21044950
REFERENCE   8  (residues 1 to 454)
  AUTHORS   Polson,H.E., de Lartigue,J., Rigden,D.J., Reedijk,M., Urbe,S.,
            Clague,M.J. and Tooze,S.A.
  TITLE     Mammalian Atg18 (WIPI2) localizes to omegasome-anchored phagophores
            and positively regulates LC3 lipidation
  JOURNAL   Autophagy 6 (4), 506-522 (2010)
   PUBMED   20505359
  REMARK    GeneRIF: A role for WIPI2 in the progression of omegasomes into
            autophagosomes, is reported.
            GeneRIF: WIPI2 is a phosphatidylinsitol-3-phosphate binding protein
            required for starvation induced autophagy.
REFERENCE   9  (residues 1 to 454)
  AUTHORS   Proikas-Cezanne,T., Waddell,S., Gaugel,A., Frickey,T., Lupas,A. and
            Nordheim,A.
  TITLE     WIPI-1alpha (WIPI49), a member of the novel 7-bladed WIPI protein
            family, is aberrantly expressed in human cancer and is linked to
            starvation-induced autophagy
  JOURNAL   Oncogene 23 (58), 9314-9325 (2004)
   PUBMED   15602573
REFERENCE   10 (residues 1 to 454)
  AUTHORS   Simpson,J.C., Wellenreuther,R., Poustka,A., Pepperkok,R. and
            Wiemann,S.
  TITLE     Systematic subcellular localization of novel proteins identified by
            large-scale cDNA sequencing
  JOURNAL   EMBO Rep 1 (3), 287-292 (2000)
   PUBMED   11256614
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL080155.1 and AC093376.5.
            
            Summary: WD40 repeat proteins are key components of many essential
            biologic functions. They regulate the assembly of multiprotein
            complexes by presenting a beta-propeller platform for simultaneous
            and reversible protein-protein interactions. Members of the WIPI
            subfamily of WD40 repeat proteins, such as WIPI2, have a 7-bladed
            propeller structure and contain a conserved motif for interaction
            with phospholipids (Proikas-Cezanne et al., 2004 [PubMed
            15602573]).[supplied by OMIM, Mar 2008].
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (a).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AL080155.1, AK023041.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000288828.9/ ENSP00000288828.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..454
                     /organism="Homo sapiens"
                     /db_xref="taxon:9606"
                     /chromosome="7"
                     /map="7p22.1"
     Protein         1..454
                     /product="WD repeat domain phosphoinositide-interacting
                     protein 2 isoform a"
                     /note="WIPI49-like protein 2; WD40 repeat protein
                     interacting with phosphoinositides 2; WD repeat domain
                     phosphoinositide-interacting protein 2"
                     /calculated_mol_wt=49277
     Region          15..60
                     /region_name="WD 1. /evidence=ECO:0000255"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9Y4P8.1)"
     Region          67..104
                     /region_name="WD 2. /evidence=ECO:0000255"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9Y4P8.1)"
     Region          110..142
                     /region_name="WD 3. /evidence=ECO:0000255"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9Y4P8.1)"
     Region          120..156
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          <136..360
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          149..189
                     /region_name="WD 4. /evidence=ECO:0000255"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9Y4P8.1)"
     Region          161..200
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          193..232
                     /region_name="WD 5. /evidence=ECO:0000255"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9Y4P8.1)"
     Region          206..246
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          238..277
                     /region_name="WD 6. /evidence=ECO:0000255"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9Y4P8.1)"
     Region          241..244
                     /region_name="L/FRRG motif.
                     /evidence=ECO:0000303|PubMed:24954904"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9Y4P8.1)"
     Region          251..284
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          292..327
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          320..359
                     /region_name="WD 7. /evidence=ECO:0000255"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9Y4P8.1)"
     Site            413
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:18669648,
                     ECO:0007744|PubMed:23186163; propagated from
                     UniProtKB/Swiss-Prot (Q9Y4P8.1)"
     CDS             1..454
                     /gene="WIPI2"
                     /gene_synonym="ATG18B; Atg21; CGI-50; IDDSSA; WIPI-2"
                     /coded_by="NM_015610.4:188..1552"
                     /note="isoform a is encoded by transcript variant 1"
                     /db_xref="CCDS:CCDS5339.1"
                     /db_xref="GeneID:26100"
                     /db_xref="HGNC:HGNC:32225"
                     /db_xref="MIM:609225"
ORIGIN      
        1 mnlasqsgea gagqllfanf nqdntevkga sraaglgrra vvwslavgsk sgykffslss
       61 vdkleqiyec tdtedvcive rlfssslvai vslkaprklk vchfkkgtei cnysysntil
      121 avklnrqrli vcleeslyih nirdmkvlht iretppnpag lcalsinndn cylaypgsat
      181 igevqvfdti nlraanmipa hdsplaalaf dasgtklata sekgtvirvf sipegqklfe
      241 frrgvkrcvs icslafsmdg mflsassnte tvhifkletv kekppeeptt wtgyfgkvlm
      301 astsylpsqv temfnqgraf atvrlpfcgh knicslatiq kiprllvgaa dgylymynld
      361 pqeggecalm kqhrldgsle ttneildsas hdcplvtqty gaaagkgtyv pssptrlayt
      421 ddlgavggac ledeasalrl dedsehppmi lrtd
//
