LOCUS       NM_175575               3417 bp    mRNA    linear   PRI 01-JUN-2024
DEFINITION  Homo sapiens WAP, follistatin/kazal, immunoglobulin, kunitz and
            netrin domain containing 2 (WFIKKN2), transcript variant 1, mRNA.
ACCESSION   NM_175575
VERSION     NM_175575.6
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3417)
  AUTHORS   Wang,Y. and Tatakis,D.N.
  TITLE     Human gingiva transcriptome during wound healing
  JOURNAL   J Clin Periodontol 44 (4), 394-402 (2017)
   PUBMED   28005267
  REMARK    GeneRIF: WFIKKN2 expression is significantly downregulated in human
            masticatory mucosa during wound healing
REFERENCE   2  (bases 1 to 3417)
  AUTHORS   Monestier,O. and Blanquet,V.
  TITLE     WFIKKN1 and WFIKKN2: 'Companion' proteins regulating TGFB activity
  JOURNAL   Cytokine Growth Factor Rev 32, 75-84 (2016)
   PUBMED   27325460
  REMARK    GeneRIF: In this review, we summarize current knowledge about the
            WFIKKN proteins and propose that they are 'companion' proteins for
            various growth factors by providing localized and sustained
            presentation of TGFB proteins to their respective receptors, thus
            regulating the balance between the activation of Smad and non-Smad
            pathways by TGFB.
            Review article
REFERENCE   3  (bases 1 to 3417)
  AUTHORS   Szlama,G., Trexler,M. and Patthy,L.
  TITLE     Latent myostatin has significant activity and this activity is
            controlled more efficiently by WFIKKN1 than by WFIKKN2
  JOURNAL   FEBS J 280 (16), 3822-3839 (2013)
   PUBMED   23829672
  REMARK    GeneRIF: Data suggest that myostatin binding to its receptor
            (activin receptors type II) can be blocked by WFIKKN1 but not
            WFIKKN2; various forms of myostatin (promyostatin, myostatin
            pro-domain, and latent/processed myostatin) bind WFIKKN1 but not
            WFIKKN2.
REFERENCE   4  (bases 1 to 3417)
  AUTHORS   Dieli-Conwright,C.M., Spektor,T.M., Rice,J.C., Sattler,F.R. and
            Schroeder,E.T.
  TITLE     Hormone therapy and maximal eccentric exercise alters
            myostatin-related gene expression in postmenopausal women
  JOURNAL   J Strength Cond Res 26 (5), 1374-1382 (2012)
   PUBMED   22395277
  REMARK    GeneRIF: After eccentric exercise, postmenopausal women not using
            hormone therapy (HT) expressed higher levels of GASP-1 while
            postmenopausal women using HT showed a significant increased
            expression over controls.
REFERENCE   5  (bases 1 to 3417)
  AUTHORS   Szlama,G., Kondas,K., Trexler,M. and Patthy,L.
  TITLE     WFIKKN1 and WFIKKN2 bind growth factors TGFbeta1, BMP2 and BMP4 but
            do not inhibit their signalling activity
  JOURNAL   FEBS J 277 (24), 5040-5050 (2010)
   PUBMED   21054789
  REMARK    GeneRIF: data indicate that WFIKKN proteins are antagonists of GDF8
            and GDF11, but in the case of TGFbeta1, BMP2 and BMP4 they function
            as growth factor binding proteins
REFERENCE   6  (bases 1 to 3417)
  AUTHORS   Clark,H.F., Gurney,A.L., Abaya,E., Baker,K., Baldwin,D., Brush,J.,
            Chen,J., Chow,B., Chui,C., Crowley,C., Currell,B., Deuel,B.,
            Dowd,P., Eaton,D., Foster,J., Grimaldi,C., Gu,Q., Hass,P.E.,
            Heldens,S., Huang,A., Kim,H.S., Klimowski,L., Jin,Y., Johnson,S.,
            Lee,J., Lewis,L., Liao,D., Mark,M., Robbie,E., Sanchez,C.,
            Schoenfeld,J., Seshagiri,S., Simmons,L., Singh,J., Smith,V.,
            Stinson,J., Vagts,A., Vandlen,R., Watanabe,C., Wieand,D., Woods,K.,
            Xie,M.H., Yansura,D., Yi,S., Yu,G., Yuan,J., Zhang,M., Zhang,Z.,
            Goddard,A., Wood,W.I., Godowski,P. and Gray,A.
  TITLE     The secreted protein discovery initiative (SPDI), a large-scale
            effort to identify novel human secreted and transmembrane proteins:
            a bioinformatics assessment
  JOURNAL   Genome Res 13 (10), 2265-2270 (2003)
   PUBMED   12975309
  REMARK    Erratum:[Genome Res. 2003 Dec;13(12):2759]
REFERENCE   7  (bases 1 to 3417)
  AUTHORS   Hill,J.J., Qiu,Y., Hewick,R.M. and Wolfman,N.M.
  TITLE     Regulation of myostatin in vivo by growth and differentiation
            factor-associated serum protein-1: a novel protein with protease
            inhibitor and follistatin domains
  JOURNAL   Mol Endocrinol 17 (6), 1144-1154 (2003)
   PUBMED   12595574
REFERENCE   8  (bases 1 to 3417)
  AUTHORS   Nagy,A., Trexler,M. and Patthy,L.
  TITLE     Expression, purification and characterization of the second
            Kunitz-type protease inhibitor domain of the human WFIKKN protein
  JOURNAL   Eur J Biochem 270 (9), 2101-2107 (2003)
   PUBMED   12709070
REFERENCE   9  (bases 1 to 3417)
  AUTHORS   Trexler,M., Banyai,L. and Patthy,L.
  TITLE     Distinct expression pattern of two related human proteins
            containing multiple types of protease-inhibitory modules
  JOURNAL   Biol Chem 383 (1), 223-228 (2002)
   PUBMED   11928817
  REMARK    GeneRIF: Newly discovered WFIKKNPR gene structure and tissue
            distribution of gene expression is commpared with that of the
            WFIKKN gene in adult and fetal tissues and organs
REFERENCE   10 (bases 1 to 3417)
  AUTHORS   Harrington,J.J., Sherf,B., Rundlett,S., Jackson,P.D., Perry,R.,
            Cain,S., Leventhal,C., Thornton,M., Ramachandran,R.,
            Whittington,J., Lerner,L., Costanzo,D., McElligott,K., Boozer,S.,
            Mays,R., Smith,E., Veloso,N., Klika,A., Hess,J., Cothren,K., Lo,K.,
            Offenbacher,J., Danzig,J. and Ducar,M.
  TITLE     Creation of genome-wide protein expression libraries using random
            activation of gene expression
  JOURNAL   Nat Biotechnol 19 (5), 440-445 (2001)
   PUBMED   11329013
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AY358142.1, AF468657.1,
            BG221308.1 and AC091062.5.
            
            On May 17, 2019 this sequence version replaced NM_175575.5.
            
            Summary: The WFIKKN1 protein contains a WAP domain, follistatin
            domain, immunoglobulin domain, two tandem Kunitz domains, and an
            NTR domain. This gene encodes a WFIKKN1-related protein which has
            the same domain organization as the WFIKKN1 protein. The WAP-type,
            follistatin type, Kunitz-type, and NTR-type protease inhibitory
            domains may control the action of multiple types of proteases.
            [provided by RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK127743.1, AY358142.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968968, SAMEA2144333
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000311378.5/ ENSP00000311184.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-443               AY358142.1         178-620
            444-2083            AF468657.1         108-1747
            2084-3335           AY358142.1         2261-3512
            3336-3412           BG221308.1         133-209
            3413-3417           AC091062.5         58358-58362         c
FEATURES             Location/Qualifiers
     source          1..3417
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="17"
                     /map="17q21.33"
     gene            1..3417
                     /gene="WFIKKN2"
                     /gene_synonym="GASP-1; hGASP-1; WFDC20B; WFIKKNRP"
                     /note="WAP, follistatin/kazal, immunoglobulin, kunitz and
                     netrin domain containing 2"
                     /db_xref="GeneID:124857"
                     /db_xref="HGNC:HGNC:30916"
                     /db_xref="MIM:610895"
     exon            1..562
                     /gene="WFIKKN2"
                     /gene_synonym="GASP-1; hGASP-1; WFDC20B; WFIKKNRP"
                     /inference="alignment:Splign:2.1.0"
     CDS             353..2083
                     /gene="WFIKKN2"
                     /gene_synonym="GASP-1; hGASP-1; WFDC20B; WFIKKNRP"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; WFIKKN-related protein; WAP, FS, Ig, two KU and
                     NTR module related protein; multivalent protease inhibitor
                     protein; WAP, kazal, immunoglobulin, kunitz and NTR
                     domain-containing protein 2; growth and differentiation
                     factor-associated serum protein 1; WAP, follistatin,
                     immunoglobulin, kunitz and NTR domain-containing-related
                     protein; WAP four-disulfide core domain 20B"
                     /codon_start=1
                     /product="WAP, Kazal, immunoglobulin, Kunitz and NTR
                     domain-containing protein 2 isoform 1 precursor"
                     /protein_id="NP_783165.1"
                     /db_xref="CCDS:CCDS11575.1"
                     /db_xref="GeneID:124857"
                     /db_xref="HGNC:HGNC:30916"
                     /db_xref="MIM:610895"
                     /translation="MWAPRCRRFWSRWEQVAALLLLLLLLGVPPRSLALPPIRYSHAG
                     ICPNDMNPNLWVDAQSTCRRECETDQECETYEKCCPNVCGTKSCVAARYMDVKGKKGP
                     VGMPKEATCDHFMCLQQGSECDIWDGQPVCKCKDRCEKEPSFTCASDGLTYYNRCYMD
                     AEACSKGITLAVVTCRYHFTWPNTSPPPPETTMHPTTASPETPELDMAAPALLNNPVH
                     QSVTMGETVSFLCDVVGRPRPEITWEKQLEDRENVVMRPNHVRGNVVVTNIAQLVIYN
                     AQLQDAGIYTCTARNVAGVLRADFPLSVVRGHQAAATSESSPNGTAFPAAECLKPPDS
                     EDCGEEQTRWHFDAQANNCLTFTFGHCHRNLNHFETYEACMLACMSGPLAACSLPALQ
                     GPCKAYAPRWAYNSQTGQCQSFVYGGCEGNGNNFESREACEESCPFPRGNQRCRACKP
                     RQKLVTSFCRSDFVILGRVSELTEEPDSGRALVTVDEVLKDEKMGLKFLGQEPLEVTL
                     LHVDWACPCPNVTVSEMPLIIMGEVDGGMAMLRPDSFVGASSARRVRKLREVMHKKTC
                     DVLKEFLGLH"
     exon            563..3417
                     /gene="WFIKKN2"
                     /gene_synonym="GASP-1; hGASP-1; WFDC20B; WFIKKNRP"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3392..3397
                     /regulatory_class="polyA_signal_sequence"
                     /gene="WFIKKN2"
                     /gene_synonym="GASP-1; hGASP-1; WFDC20B; WFIKKNRP"
                     /note="hexamer: ATTAAA"
     polyA_site      3417
                     /gene="WFIKKN2"
                     /gene_synonym="GASP-1; hGASP-1; WFDC20B; WFIKKNRP"
                     /note="major polyA site"
ORIGIN      
        1 agaggcgcca gcagcctgcc tgtgacaggc atcaggttag ctggctccca ctcgggtggc
       61 gcgcccagga tataaatccg ggcgcgggcc cctgctgtgg ctcctctccc tgcacactca
      121 ggagagggag cttccttcta aagacctttc ttttatctga agccgcacag cccggcaggc
      181 tgtgctgact tggtggaggc agcagcggca gagcagcctg agcagcagcc tgagcaggaa
      241 acctgctggg gtggggaggg caggtgtctg cagcccctga gaagaaggcc ctggtgggcc
      301 ccagaccctg gcatcgtttc aggggaggtc tctagccgcc ccagcctgca ccatgtgggc
      361 cccaaggtgt cgccggttct ggtctcgctg ggagcaggtg gcagcgctgc tgctgctgct
      421 gctactgctc ggggtgcccc cgcgaagcct ggcgctgccg cccatccgct attcccacgc
      481 cggcatctgc cccaacgaca tgaatcccaa cctctgggtg gacgcacaga gcacctgcag
      541 gcgggagtgt gagacggacc aggagtgtga gacctatgag aagtgctgcc ccaacgtatg
      601 tgggaccaag agctgcgtgg cggcccgcta catggacgtg aaagggaaga agggcccagt
      661 gggcatgccc aaggaggcca catgtgacca cttcatgtgt ctgcagcagg gctctgagtg
      721 tgacatctgg gatggccagc ccgtgtgtaa gtgcaaagac cgctgtgaga aggagcccag
      781 ctttacctgc gcctcggacg gcctcaccta ctataaccgc tgctacatgg atgccgaggc
      841 ctgctccaaa ggcatcacac tggccgttgt aacctgccgc tatcacttca cctggcccaa
      901 caccagcccc ccaccacctg agaccaccat gcaccccacc acagcctccc cagagacccc
      961 tgagctggac atggcggccc ctgcgctgct caacaaccct gtgcaccagt cggtcaccat
     1021 gggtgagaca gtgagcttcc tctgtgatgt ggtgggccgg ccccggcctg agatcacctg
     1081 ggagaagcag ttggaggatc gggagaatgt ggtcatgcgg cccaaccatg tgcgtggcaa
     1141 cgtggtggtc accaacattg cccagctggt catctataac gcccagctgc aggatgctgg
     1201 gatctacacc tgcacggccc ggaacgtggc tggggtcctg agggctgatt tcccgctgtc
     1261 ggtggtcagg ggtcatcagg ctgcagccac ctcagagagc agccccaatg gcacggcttt
     1321 cccggcggcc gagtgcctga agcccccaga cagtgaggac tgtggcgaag agcagacccg
     1381 ctggcacttc gatgcccagg ccaacaactg cctgaccttc accttcggcc actgccaccg
     1441 taacctcaac cactttgaga cctatgaggc ctgcatgctg gcctgcatga gcgggccgct
     1501 ggccgcgtgc agcctgcccg ccctgcaggg gccctgcaaa gcctacgcgc ctcgctgggc
     1561 ttacaacagc cagacgggcc agtgccagtc ctttgtctat ggtggctgcg agggcaatgg
     1621 caacaacttt gagagccgtg aggcctgtga ggagtcgtgc cccttcccca gggggaacca
     1681 gcgctgtcgg gcctgcaagc ctcggcagaa gctcgttacc agcttctgtc gcagcgactt
     1741 tgtcatcctg ggccgagtct ctgagctgac cgaggagcct gactcgggcc gcgccctggt
     1801 gactgtggat gaggtcctaa aggatgagaa aatgggcctc aagttcctgg gccaggagcc
     1861 attggaggtc actctgcttc acgtggactg ggcatgcccc tgccccaacg tgaccgtgag
     1921 cgagatgccg ctcatcatca tgggggaggt ggacggcggc atggccatgc tgcgccccga
     1981 tagctttgtg ggcgcatcga gtgcccgccg ggtcaggaag cttcgtgagg tcatgcacaa
     2041 gaagacctgt gacgtcctca aggagtttct tggcttgcac tgaagccccc cacccctccc
     2101 tgccccctcc ctggccttct tccacctatc caccccaatg cctctcagca aactgggcga
     2161 ggtcagatta gacaggcttg ggacagcagg gaaacatcaa ccgacgtgtc acagaaaaag
     2221 ccacagaagg tctcagatca gcatctattc tttgggttca ataaggggtt catatctttt
     2281 ttagctgagg gggacaagag gagaagtcag tggacacatg gaagttactc gtgaccacca
     2341 gcttgctcag atattctcct cctcccctca ctggccccac acccctggct ctcccagtca
     2401 ccctccccta gccagtctcc cagcaagggt ttaagagatg gccgctgtgt gctggtcaca
     2461 ggaagtgttg aatggattgg cttgcaaagg gggtaggtgg ggagagatag gagggcccag
     2521 ggactcatgg gacacctttc ccacagcctc ctcgattgct gtgagcagag gccactcgga
     2581 gttaggggca tgggcaatag caagctggcg gcagagtcca gcccagcata tgacttgccc
     2641 tgaatggaag ctgctgaaac gggtgccttt gggtggtggt cggcttgcct ctgaggccac
     2701 cacggcacca gcagaatacg tatttcttct ccttggctgc actggtttgt cgatctagtt
     2761 cagttcaact cagtggatgt tctctgaatg cttactgggt gccaggacca cagagagatg
     2821 ttagtcactg cccagttctt agagccccaa cacagatacc ctcatcccag ggcccccaga
     2881 cacacccctc cgctggactc acaactgtct ggagtttctg tctgatggat ggtgtgcttt
     2941 catatgccac tggcttcctt ggacatagat cagacaaaag ccccgggatc tgtttggtag
     3001 caggagaaat gaaggaagat gaaaaagcag gcagggaagg gggtagtaaa ggactgagag
     3061 aggagggagg tggctggaga aggaaaagga acattgctcg atgctcccat ctggtggcgg
     3121 cctcaggaac ccacgggaac ctggaaggag gctctttgtg agacctgggc aaaggatggg
     3181 gcagctcgtc gatgattttt ttgtgtttcc aggcttcctg tgtgatcctg gccctccggc
     3241 cgctagagag aggattggga aaccccactg tcagctctgc atctgccccc actaccctcc
     3301 tctgccctat tctgtccctg cccctccaag ctgaagaagg tccttgtggg gcgtcctcat
     3361 ttcttcctca aatataagga ggaagatacc aattaaaagc tcatagtatc aactgcc
//
