LOCUS       NM_053284               1976 bp    mRNA    linear   PRI 01-JUN-2024
DEFINITION  Homo sapiens WAP, follistatin/kazal, immunoglobulin, kunitz and
            netrin domain containing 1 (WFIKKN1), mRNA.
ACCESSION   NM_053284
VERSION     NM_053284.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1976)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   2  (bases 1 to 1976)
  AUTHORS   Szlama,G., Vasarhelyi,V., Trexler,M. and Patthy,L.
  TITLE     Influence of WFIKKN1 on BMP1-mediated activation of latent
            myostatin
  JOURNAL   FEBS J 283 (24), 4515-4527 (2016)
   PUBMED   27782377
  REMARK    GeneRIF: Data show that the KKN1 fragment generated by
            BMP1-cleavage of WFIKKN1 protein contributes most significantly to
            the observed enhancer activity.
REFERENCE   3  (bases 1 to 1976)
  AUTHORS   Monestier,O. and Blanquet,V.
  TITLE     WFIKKN1 and WFIKKN2: 'Companion' proteins regulating TGFB activity
  JOURNAL   Cytokine Growth Factor Rev 32, 75-84 (2016)
   PUBMED   27325460
  REMARK    GeneRIF: In this review, we summarize current knowledge about the
            WFIKKN proteins and propose that they are 'companion' proteins for
            various growth factors by providing localized and sustained
            presentation of TGFB proteins to their respective receptors, thus
            regulating the balance between the activation of Smad and non-Smad
            pathways by TGFB.
            Review article
REFERENCE   4  (bases 1 to 1976)
  AUTHORS   Szlama,G., Trexler,M. and Patthy,L.
  TITLE     Latent myostatin has significant activity and this activity is
            controlled more efficiently by WFIKKN1 than by WFIKKN2
  JOURNAL   FEBS J 280 (16), 3822-3839 (2013)
   PUBMED   23829672
  REMARK    GeneRIF: Data suggest that myostatin binding to its receptor
            (activin receptors type II) can be blocked by WFIKKN1 but not
            WFIKKN2; various forms of myostatin (promyostatin, myostatin
            pro-domain, and latent/processed myostatin) bind WFIKKN1 but not
            WFIKKN2.
REFERENCE   5  (bases 1 to 1976)
  AUTHORS   Szlama,G., Kondas,K., Trexler,M. and Patthy,L.
  TITLE     WFIKKN1 and WFIKKN2 bind growth factors TGFbeta1, BMP2 and BMP4 but
            do not inhibit their signalling activity
  JOURNAL   FEBS J 277 (24), 5040-5050 (2010)
   PUBMED   21054789
  REMARK    GeneRIF: data indicate that WFIKKN proteins are antagonists of GDF8
            and GDF11, but in the case of TGFbeta1, BMP2 and BMP4 they function
            as growth factor binding proteins
REFERENCE   6  (bases 1 to 1976)
  AUTHORS   Liepinsh,E., Nagy,A., Trexler,M., Patthy,L. and Otting,G.
  TITLE     Second Kunitz-type protease inhibitor domain of the human WFIKKN1
            protein
  JOURNAL   J Biomol NMR 35 (1), 73-78 (2006)
   PUBMED   16791741
  REMARK    GeneRIF: three-dimensional structure of a Kunitz domain with a
            tryptophan residue at the P02 site of the protease-recognition loop
REFERENCE   7  (bases 1 to 1976)
  AUTHORS   Nagy,A., Trexler,M. and Patthy,L.
  TITLE     Expression, purification and characterization of the second
            Kunitz-type protease inhibitor domain of the human WFIKKN protein
  JOURNAL   Eur J Biochem 270 (9), 2101-2107 (2003)
   PUBMED   12709070
  REMARK    GeneRIF: Data suggest that the physiological target of WFIKKN
            protein may be trypsin.
REFERENCE   8  (bases 1 to 1976)
  AUTHORS   Trexler,M., Banyai,L. and Patthy,L.
  TITLE     Distinct expression pattern of two related human proteins
            containing multiple types of protease-inhibitory modules
  JOURNAL   Biol Chem 383 (1), 223-228 (2002)
   PUBMED   11928817
  REMARK    GeneRIF: Newly discovered WFIKKNPR gene structure and tissue
            distribution of gene expression is commpared with that of the
            WFIKKN gene in adult and fetal tissues and organs.
REFERENCE   9  (bases 1 to 1976)
  AUTHORS   Trexler,M., Banyai,L. and Patthy,L.
  TITLE     A human protein containing multiple types of protease-inhibitory
            modules
  JOURNAL   Proc Natl Acad Sci U S A 98 (7), 3705-3709 (2001)
   PUBMED   11274388
REFERENCE   10 (bases 1 to 1976)
  AUTHORS   Daniels,R.J., Peden,J.F., Lloyd,C., Horsley,S.W., Clark,K.,
            Tufarelli,C., Kearney,L., Buckle,V.J., Doggett,N.A., Flint,J. and
            Higgs,D.R.
  TITLE     Sequence, structure and pathology of the fully annotated terminal 2
            Mb of the short arm of human chromosome 16
  JOURNAL   Hum Mol Genet 10 (4), 339-352 (2001)
   PUBMED   11157797
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from Z84479.1, CN425794.1,
            AF422194.1 and AK075356.1.
            
            On Nov 22, 2018 this sequence version replaced NM_053284.2.
            
            Summary: This gene encodes a secreted multidomain protein
            consisting of a signal peptide, a WAP domain, a follistatin domain,
            an immunoglobulin domain, two tandem Kunitz domains, and an NTR
            domain. These domains have been implicated frequently in inhibition
            of various types of proteases, suggesting that the encoded protein
            may be a multivalent protease inhibitor and may control the action
            of multiple types of serine proteases as well as
            metalloproteinases. [provided by RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK075356.1, AK314757.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968540, SAMEA1968832
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000319070.3/ ENSP00000324763.2
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-27                Z84479.1           26324-26350
            28-269              CN425794.1         12-253
            270-1948            AF422194.1         1-1679
            1949-1975           AK075356.1         2001-2027
            1976-1976           Z84479.1           29456-29456
FEATURES             Location/Qualifiers
     source          1..1976
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="16"
                     /map="16p13.3"
     gene            1..1976
                     /gene="WFIKKN1"
                     /gene_synonym="C16orf12; RJD2; WFDC20A; WFIKKN"
                     /note="WAP, follistatin/kazal, immunoglobulin, kunitz and
                     netrin domain containing 1"
                     /db_xref="GeneID:117166"
                     /db_xref="HGNC:HGNC:30912"
                     /db_xref="MIM:608021"
     exon            1..440
                     /gene="WFIKKN1"
                     /gene_synonym="C16orf12; RJD2; WFDC20A; WFIKKN"
                     /inference="alignment:Splign:2.1.0"
     CDS             270..1916
                     /gene="WFIKKN1"
                     /gene_synonym="C16orf12; RJD2; WFDC20A; WFIKKN"
                     /note="WAP, FS, Ig, KU, and NTR-containing protein; WAP,
                     kazal, immunoglobulin, kunitz and NTR domain-containing
                     protein 1; WAP, follistatin, immunoglobulin, kunitz and
                     NTR domain-containing protein; GASP-2; hGASP-2; growth and
                     differentiation factor-associated serum protein 2; WAP
                     four-disulfide core domain 20A"
                     /codon_start=1
                     /product="WAP, Kazal, immunoglobulin, Kunitz and NTR
                     domain-containing protein 1 precursor"
                     /protein_id="NP_444514.1"
                     /db_xref="CCDS:CCDS10414.1"
                     /db_xref="GeneID:117166"
                     /db_xref="HGNC:HGNC:30912"
                     /db_xref="MIM:608021"
                     /translation="MPALRPLLPLLLLLRLTSGAGLLPGLGSHPGVCPNQLSPNLWVD
                     AQSTCERECSRDQDCAAAEKCCINVCGLHSCVAARFPGSPAAPTTAASCEGFVCPQQG
                     SDCDIWDGQPVCRCRDRCEKEPSFTCASDGLTYYNRCYMDAEACLRGLHLHIVPCKHV
                     LSWPPSSPGPPETTARPTPGAAPVPPALYSSPSPQAVQVGGTASLHCDVSGRPPPAVT
                     WEKQSHQRENLIMRPDQMYGNVVVTSIGQLVLYNARPEDAGLYTCTARNAAGLLRADF
                     PLSVVQREPARDAAPSIPAPAECLPDVQACTGPTSPHLVLWHYDPQRGGCMTFPARGC
                     DGAARGFETYEACQQACARGPGDACVLPAVQGPCRGWEPRWAYSPLLQQCHPFVYGGC
                     EGNGNNFHSRESCEDACPVPRTPPCRACRLRSKLALSLCRSDFAIVGRLTEVLEEPEA
                     AGGIARVALEDVLKDDKMGLKFLGTKYLEVTLSGMDWACPCPNMTAGDGPLVIMGEVR
                     DGVAVLDAGSYVRAASEKRVKKILELLEKQACELLNRFQD"
     exon            441..1976
                     /gene="WFIKKN1"
                     /gene_synonym="C16orf12; RJD2; WFDC20A; WFIKKN"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1951..1956
                     /regulatory_class="polyA_signal_sequence"
                     /gene="WFIKKN1"
                     /gene_synonym="C16orf12; RJD2; WFDC20A; WFIKKN"
                     /note="hexamer: AATAAA"
     polyA_site      1976
                     /gene="WFIKKN1"
                     /gene_synonym="C16orf12; RJD2; WFDC20A; WFIKKN"
                     /note="major polyA site"
ORIGIN      
        1 agacagaggg gccaggctga agctggagag gaaccagcgt cacacagacg gcctctgaga
       61 acttggagac cccgttaccc acccagcagg ggtgtcagga caagcatctg ctgcaggctt
      121 cagcctcagg ggcaaaaggg agccccgggg tcctggtggg ggcaccgacc acaggcccgg
      181 agggtggatg cctgcaggaa gctgggctct gtggagcccg aggaggggct ggtggccaca
      241 ccccccggcc ccctggctcg gcggccctca tgcccgccct acgtccactc ctgccgctcc
      301 tgctcctcct ccggctgacc tcgggggctg gcttgctgcc agggctgggg agccacccgg
      361 gcgtgtgccc caaccagctc agccccaacc tgtgggtgga cgcccagagc acctgtgagc
      421 gcgagtgtag cagggaccag gactgtgcgg ctgctgagaa gtgctgcatc aacgtgtgtg
      481 gactgcacag ctgcgtggca gcacgcttcc ccggcagccc agctgcgccg acgacagcgg
      541 cctcctgcga gggctttgtg tgcccacagc agggctcgga ctgcgacatc tgggacgggc
      601 agcccgtgtg ccgctgccgc gaccgctgtg agaaggagcc cagcttcacc tgcgcctcgg
      661 acggcctcac ctactacaac cgctgctata tggacgccga ggcctgcctg cggggcctgc
      721 acctccacat cgtgccctgc aagcacgtgc tcagctggcc gcccagcagc ccggggccgc
      781 cggagaccac tgcccgcccc acacctgggg ccgcgcccgt gcctcctgcc ctgtacagca
      841 gcccctcccc acaggcggtg caggttgggg gtacggccag cctccactgc gacgtcagcg
      901 gccgcccgcc gcctgctgtg acctgggaga agcagagtca ccagcgagag aacctgatca
      961 tgcgccctga tcagatgtat ggcaacgtgg tggtcaccag catcgggcag ctggtgctct
     1021 acaacgcgcg gcccgaagac gccggcctgt acacctgcac cgcgcgcaac gctgctgggc
     1081 tgctgcgggc tgacttccca ctctctgtgg tccagcgaga gccggccagg gacgcagccc
     1141 ccagcatccc agccccggcc gagtgcctgc cggatgtgca ggcctgcacg ggccccactt
     1201 ccccacacct tgtcctctgg cactacgacc cgcagcgggg cggctgcatg accttcccgg
     1261 cccgtggctg tgatggggcg gcccgcggct ttgagaccta cgaggcatgc cagcaggcct
     1321 gtgcccgcgg ccccggcgac gcctgcgtgc tgcctgccgt gcagggcccc tgccggggct
     1381 gggagccgcg ctgggcctac agcccgctgc tgcagcagtg ccatcccttc gtgtacggtg
     1441 gctgcgaggg caacggcaac aacttccaca gccgcgagag ctgcgaggat gcctgccccg
     1501 tgccgcgcac accgccctgc cgcgcctgcc gcctccggag caagctggcg ctgagcctgt
     1561 gccgcagcga cttcgccatc gtggggcggc tcacggaggt gctggaggag cccgaggccg
     1621 ccggcggcat cgcccgcgtg gcgctcgagg acgtgctcaa ggatgacaag atgggcctca
     1681 agttcttggg caccaagtac ctggaggtga cgctgagtgg catggactgg gcctgcccct
     1741 gccccaacat gacggcgggc gacgggccgc tggtcatcat gggtgaggtg cgcgatggcg
     1801 tggccgtgct ggacgccggc agctacgtcc gcgccgccag cgagaagcgc gtcaagaaga
     1861 tcttggagct gctggagaag caggcctgcg agctgctcaa ccgcttccag gactagcccc
     1921 cgcaggggcc tgcgccaccc cgtcctggtg aataaacgca ctccctgtgc ctcaga
//
