LOCUS       NM_130896               1027 bp    mRNA    linear   PRI 06-APR-2024
DEFINITION  Homo sapiens WAP four-disulfide core domain 8 (WFDC8), transcript
            variant a, mRNA.
ACCESSION   NM_130896
VERSION     NM_130896.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1027)
  AUTHORS   Ferreira,Z., Seixas,S., Andres,A.M., Kretzschmar,W.W.,
            Mullikin,J.C., Cherukuri,P.F., Cruz,P., Swanson,W.J., Clark,A.G.,
            Green,E.D. and Hurle,B.
  CONSRTM   NISC Comparative Sequencing Program
  TITLE     Reproduction and immunity-driven natural selection in the human
            WFDC locus
  JOURNAL   Mol Biol Evol 30 (4), 938-950 (2013)
   PUBMED   23292442
REFERENCE   2  (bases 1 to 1027)
  AUTHORS   Ferreira,Z., Hurle,B., Rocha,J. and Seixas,S.
  TITLE     Differing evolutionary histories of WFDC8 (short-term balancing) in
            Europeans and SPINT4 (incomplete selective sweep) in Africans
  JOURNAL   Mol Biol Evol 28 (10), 2811-2822 (2011)
   PUBMED   21536719
  REMARK    GeneRIF: We propose that the evolution of WFDC8 and SPINT4 has been
            shaped by complex selective scenarios due to the interdependence of
            variant fitness and ecological variables.
REFERENCE   3  (bases 1 to 1027)
  AUTHORS   Clauss,A., Lilja,H. and Lundwall,A.
  TITLE     The evolution of a genetic locus encoding small serine proteinase
            inhibitors
  JOURNAL   Biochem Biophys Res Commun 333 (2), 383-389 (2005)
   PUBMED   15950183
REFERENCE   4  (bases 1 to 1027)
  AUTHORS   Clauss,A., Lilja,H. and Lundwall,A.
  TITLE     A locus on human chromosome 20 contains several genes expressing
            protease inhibitor domains with homology to whey acidic protein
  JOURNAL   Biochem J 368 (Pt 1), 233-242 (2002)
   PUBMED   12206714
REFERENCE   5  (bases 1 to 1027)
  AUTHORS   Deloukas,P., Matthews,L.H., Ashurst,J., Burton,J., Gilbert,J.G.,
            Jones,M., Stavrides,G., Almeida,J.P., Babbage,A.K., Bagguley,C.L.,
            Bailey,J., Barlow,K.F., Bates,K.N., Beard,L.M., Beare,D.M.,
            Beasley,O.P., Bird,C.P., Blakey,S.E., Bridgeman,A.M., Brown,A.J.,
            Buck,D., Burrill,W., Butler,A.P., Carder,C., Carter,N.P.,
            Chapman,J.C., Clamp,M., Clark,G., Clark,L.N., Clark,S.Y.,
            Clee,C.M., Clegg,S., Cobley,V.E., Collier,R.E., Connor,R.,
            Corby,N.R., Coulson,A., Coville,G.J., Deadman,R., Dhami,P.,
            Dunn,M., Ellington,A.G., Frankland,J.A., Fraser,A., French,L.,
            Garner,P., Grafham,D.V., Griffiths,C., Griffiths,M.N., Gwilliam,R.,
            Hall,R.E., Hammond,S., Harley,J.L., Heath,P.D., Ho,S., Holden,J.L.,
            Howden,P.J., Huckle,E., Hunt,A.R., Hunt,S.E., Jekosch,K.,
            Johnson,C.M., Johnson,D., Kay,M.P., Kimberley,A.M., King,A.,
            Knights,A., Laird,G.K., Lawlor,S., Lehvaslaiho,M.H., Leversha,M.,
            Lloyd,C., Lloyd,D.M., Lovell,J.D., Marsh,V.L., Martin,S.L.,
            McConnachie,L.J., McLay,K., McMurray,A.A., Milne,S., Mistry,D.,
            Moore,M.J., Mullikin,J.C., Nickerson,T., Oliver,K., Parker,A.,
            Patel,R., Pearce,T.A., Peck,A.I., Phillimore,B.J.,
            Prathalingam,S.R., Plumb,R.W., Ramsay,H., Rice,C.M., Ross,M.T.,
            Scott,C.E., Sehra,H.K., Shownkeen,R., Sims,S., Skuce,C.D.,
            Smith,M.L., Soderlund,C., Steward,C.A., Sulston,J.E., Swann,M.,
            Sycamore,N., Taylor,R., Tee,L., Thomas,D.W., Thorpe,A., Tracey,A.,
            Tromans,A.C., Vaudin,M., Wall,M., Wallis,J.M., Whitehead,S.L.,
            Whittaker,P., Willey,D.L., Williams,L., Williams,S.A., Wilming,L.,
            Wray,P.W., Hubbard,T., Durbin,R.M., Bentley,D.R., Beck,S. and
            Rogers,J.
  TITLE     The DNA sequence and comparative analysis of human chromosome 20
  JOURNAL   Nature 414 (6866), 865-871 (2001)
   PUBMED   11780052
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL121778.12 and AL031663.2.
            
            On Jun 2, 2019 this sequence version replaced NM_130896.2.
            
            Summary: This gene encodes a member of the WAP-type four-disulfide
            core (WFDC) domain family. The WFDC domain, or WAP signature motif,
            contains eight cysteines forming four disulfide bonds at the core
            of the protein, and functions as a protease inhibitor. The encoded
            protein contains a Kunitz-inhibitor domain, in addition to three
            WFDC domains. Most WFDC genes are localized to chromosome 20q12-q13
            in two clusters: centromeric and telomeric. This gene belongs to
            the telomeric cluster. Two alternatively spliced transcript
            variants have been found for this gene, and they encode the same
            protein. [provided by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (a) represents the shorter
            transcript.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: HM005608.1, SRR5189667.371189.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2159931 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000289953.3/ ENSP00000289953.2
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-63                AL121778.12        16046-16108         c
            64-173              AL031663.2         125861-125970       c
            174-314             AL031663.2         122603-122743       c
            315-482             AL031663.2         119452-119619       c
            483-623             AL031663.2         116887-117027       c
            624-1027            AL031663.2         115513-115916       c
FEATURES             Location/Qualifiers
     source          1..1027
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="20"
                     /map="20q13.12"
     gene            1..1027
                     /gene="WFDC8"
                     /gene_synonym="C20orf170; dJ461P17.1; HEL-S-292; WAP8"
                     /note="WAP four-disulfide core domain 8"
                     /db_xref="GeneID:90199"
                     /db_xref="HGNC:HGNC:16163"
     exon            1..63
                     /gene="WFDC8"
                     /gene_synonym="C20orf170; dJ461P17.1; HEL-S-292; WAP8"
                     /inference="alignment:Splign:2.1.0"
     CDS             38..763
                     /gene="WFDC8"
                     /gene_synonym="C20orf170; dJ461P17.1; HEL-S-292; WAP8"
                     /note="protease inhibitor WAP8; WAP four-disulfide core
                     domain protein 8; WAP motif protein 1; putative protease
                     inhibitor WAP8; epididymis secretory protein Li 292;
                     testicular secretory protein Li 68; epididymis secretory
                     sperm binding protein"
                     /codon_start=1
                     /product="WAP four-disulfide core domain protein 8
                     precursor"
                     /protein_id="NP_570966.2"
                     /db_xref="CCDS:CCDS13361.1"
                     /db_xref="GeneID:90199"
                     /db_xref="HGNC:HGNC:16163"
                     /translation="MWTVRTEGGHFPLHSPTFSWRNVAFLLLLSLALEWTSAMLTKKI
                     KHKPGLCPKERLTCTTELPDSCNTDFDCKEYQKCCFFACQKKCMDPFQEPCMLPVRHG
                     NCNHEAQRWHFDFKNYRCTPFKYRGCEGNANNFLNEDACRTACMLIVKDGQCPLFPFT
                     ERKECPPSCHSDIDCPQTDKCCESRCGFVCARAWTVKKGFCPRKPLLCTKIDKPKCLQ
                     DEECPLVEKCCSHCGLKCMDPRR"
     exon            64..173
                     /gene="WFDC8"
                     /gene_synonym="C20orf170; dJ461P17.1; HEL-S-292; WAP8"
                     /inference="alignment:Splign:2.1.0"
     exon            174..314
                     /gene="WFDC8"
                     /gene_synonym="C20orf170; dJ461P17.1; HEL-S-292; WAP8"
                     /inference="alignment:Splign:2.1.0"
     exon            315..482
                     /gene="WFDC8"
                     /gene_synonym="C20orf170; dJ461P17.1; HEL-S-292; WAP8"
                     /inference="alignment:Splign:2.1.0"
     exon            483..623
                     /gene="WFDC8"
                     /gene_synonym="C20orf170; dJ461P17.1; HEL-S-292; WAP8"
                     /inference="alignment:Splign:2.1.0"
     exon            624..1027
                     /gene="WFDC8"
                     /gene_synonym="C20orf170; dJ461P17.1; HEL-S-292; WAP8"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 caaagtgagg aagctgtctc catagggaag aggcctgatg tggactgtcc gaactgaagg
       61 agggcacttt cctctccata gccccacctt ctcctggagg aatgtagctt tcctgctgct
      121 tctctccctt gctttggagt ggacttctgc aatgctgacc aagaagatca aacacaaacc
      181 agggttatgt cccaaagaga ggctcacctg taccactgaa cttccggact catgtaacac
      241 agattttgac tgcaaggaat accagaagtg ctgctttttt gcctgtcaga agaagtgcat
      301 ggatcccttt caagaaccct gcatgctacc tgtgaggcat ggaaactgta atcatgaggc
      361 acagcgctgg cattttgact ttaaaaatta ccgctgcaca cccttcaaat acaggggctg
      421 cgaagggaat gccaacaact tcttaaatga ggatgcctgc agaacggcct gcatgttaat
      481 tgttaaggat ggacaatgcc cactcttccc tttcactgaa cgtaaggagt gtccaccttc
      541 atgtcacagt gacatcgatt gtccccagac agacaaatgt tgtgaatcca ggtgtggctt
      601 tgtttgtgcc agggcctgga cagtcaaaaa aggtttctgc ccacgcaagc ccttgctatg
      661 taccaagatt gataaaccca agtgcctgca ggatgaggag tgcccattgg tggaaaagtg
      721 ctgctcacat tgtggactga aatgtatgga ccccagacgt tgaatagtaa gtagaaaata
      781 ttatcatcaa aatcattaat tatgagtagc atgataaaga gctgtcagtt ttgtcttgta
      841 tccaacttgc caaattatct taagtatcac aatattgaat gatgttactt tgattttata
      901 tataatccca tcatctgaaa gtgatgatat atttggcttt ttttcaactt catgacttta
      961 ttttcttgtc ttcttgtatt gtgtagaacc tccaaaataa taatgaaaat aaaggcaatg
     1021 gcatcca
//
