LOCUS       NM_145652               1009 bp    mRNA    linear   PRI 25-JUL-2022
DEFINITION  Homo sapiens WAP four-disulfide core domain 5 (WFDC5), transcript
            variant 1, mRNA.
ACCESSION   NM_145652
VERSION     NM_145652.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1009)
  AUTHORS   Haenig C, Atias N, Taylor AK, Mazza A, Schaefer MH, Russ J,
            Riechers SP, Jain S, Coughlin M, Fontaine JF, Freibaum BD,
            Brusendorf L, Zenkner M, Porras P, Stroedicke M, Schnoegl S,
            Arnsburg K, Boeddrich A, Pigazzini L, Heutink P, Taylor JP,
            Kirstein J, Andrade-Navarro MA, Sharan R and Wanker EE.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   2  (bases 1 to 1009)
  AUTHORS   Luck K, Kim DK, Lambourne L, Spirohn K, Begg BE, Bian W, Brignall
            R, Cafarelli T, Campos-Laborie FJ, Charloteaux B, Choi D, Cote AG,
            Daley M, Deimling S, Desbuleux A, Dricot A, Gebbia M, Hardy MF,
            Kishore N, Knapp JJ, Kovacs IA, Lemmens I, Mee MW, Mellor JC,
            Pollis C, Pons C, Richardson AD, Schlabach S, Teeking B, Yadav A,
            Babor M, Balcha D, Basha O, Bowman-Colin C, Chin SF, Choi SG,
            Colabella C, Coppin G, D'Amata C, De Ridder D, De Rouck S,
            Duran-Frigola M, Ennajdaoui H, Goebels F, Goehring L, Gopal A,
            Haddad G, Hatchi E, Helmy M, Jacob Y, Kassa Y, Landini S, Li R, van
            Lieshout N, MacWilliams A, Markey D, Paulson JN, Rangarajan S,
            Rasla J, Rayhan A, Rolland T, San-Miguel A, Shen Y, Sheykhkarimli
            D, Sheynkman GM, Simonovsky E, Tasan M, Tejeda A, Tropepe V,
            Twizere JC, Wang Y, Weatheritt RJ, Weile J, Xia Y, Yang X,
            Yeger-Lotem E, Zhong Q, Aloy P, Bader GD, De Las Rivas J, Gaudet S,
            Hao T, Rak J, Tavernier J, Hill DE, Vidal M, Roth FP and Calderwood
            MA.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   3  (bases 1 to 1009)
  AUTHORS   Ferreira Z, Seixas S, Andres AM, Kretzschmar WW, Mullikin JC,
            Cherukuri PF, Cruz P, Swanson WJ, Clark AG, Green ED and Hurle B.
  CONSRTM   NISC Comparative Sequencing Program
  TITLE     Reproduction and immunity-driven natural selection in the human
            WFDC locus
  JOURNAL   Mol Biol Evol 30 (4), 938-950 (2013)
   PUBMED   23292442
REFERENCE   4  (bases 1 to 1009)
  AUTHORS   Mukhopadhyay SS and Rosen JM.
  TITLE     The C-terminal domain of the nuclear factor I-B2 isoform is
            glycosylated and transactivates the WAP gene in the JEG-3 cells
  JOURNAL   Biochem Biophys Res Commun 358 (3), 770-776 (2007)
   PUBMED   17511965
  REMARK    GeneRIF: C-terminal domain of the nuclear factor I-B2 isoform is
            glycosylated and transactivates the WAP gene in tumor cells
REFERENCE   5  (bases 1 to 1009)
  AUTHORS   Nukumi N, Seki M, Iwamori T, Yada T, Naito K and Tojo H.
  TITLE     Analysis of the promoter of mutated human whey acidic protein (WAP)
            gene
  JOURNAL   J Reprod Dev 52 (2), 315-320 (2006)
   PUBMED   16462094
  REMARK    GeneRIF: analysis of promoter of mutated human whey acidic protein
            (WAP) gene
REFERENCE   6  (bases 1 to 1009)
  AUTHORS   Clark HF, Gurney AL, Abaya E, Baker K, Baldwin D, Brush J, Chen J,
            Chow B, Chui C, Crowley C, Currell B, Deuel B, Dowd P, Eaton D,
            Foster J, Grimaldi C, Gu Q, Hass PE, Heldens S, Huang A, Kim HS,
            Klimowski L, Jin Y, Johnson S, Lee J, Lewis L, Liao D, Mark M,
            Robbie E, Sanchez C, Schoenfeld J, Seshagiri S, Simmons L, Singh J,
            Smith V, Stinson J, Vagts A, Vandlen R, Watanabe C, Wieand D, Woods
            K, Xie MH, Yansura D, Yi S, Yu G, Yuan J, Zhang M, Zhang Z, Goddard
            A, Wood WI, Godowski P and Gray A.
  TITLE     The secreted protein discovery initiative (SPDI), a large-scale
            effort to identify novel human secreted and transmembrane proteins:
            a bioinformatics assessment
  JOURNAL   Genome Res 13 (10), 2265-2270 (2003)
   PUBMED   12975309
  REMARK    Erratum:[Genome Res. 2003 Dec;13(12):2759]
REFERENCE   7  (bases 1 to 1009)
  AUTHORS   Clauss A, Lilja H and Lundwall A.
  TITLE     A locus on human chromosome 20 contains several genes expressing
            protease inhibitor domains with homology to whey acidic protein
  JOURNAL   Biochem J 368 (Pt 1), 233-242 (2002)
   PUBMED   12206714
REFERENCE   8  (bases 1 to 1009)
  AUTHORS   Deloukas P, Matthews LH, Ashurst J, Burton J, Gilbert JG, Jones M,
            Stavrides G, Almeida JP, Babbage AK, Bagguley CL, Bailey J, Barlow
            KF, Bates KN, Beard LM, Beare DM, Beasley OP, Bird CP, Blakey SE,
            Bridgeman AM, Brown AJ, Buck D, Burrill W, Butler AP, Carder C,
            Carter NP, Chapman JC, Clamp M, Clark G, Clark LN, Clark SY, Clee
            CM, Clegg S, Cobley VE, Collier RE, Connor R, Corby NR, Coulson A,
            Coville GJ, Deadman R, Dhami P, Dunn M, Ellington AG, Frankland JA,
            Fraser A, French L, Garner P, Grafham DV, Griffiths C, Griffiths
            MN, Gwilliam R, Hall RE, Hammond S, Harley JL, Heath PD, Ho S,
            Holden JL, Howden PJ, Huckle E, Hunt AR, Hunt SE, Jekosch K,
            Johnson CM, Johnson D, Kay MP, Kimberley AM, King A, Knights A,
            Laird GK, Lawlor S, Lehvaslaiho MH, Leversha M, Lloyd C, Lloyd DM,
            Lovell JD, Marsh VL, Martin SL, McConnachie LJ, McLay K, McMurray
            AA, Milne S, Mistry D, Moore MJ, Mullikin JC, Nickerson T, Oliver
            K, Parker A, Patel R, Pearce TA, Peck AI, Phillimore BJ,
            Prathalingam SR, Plumb RW, Ramsay H, Rice CM, Ross MT, Scott CE,
            Sehra HK, Shownkeen R, Sims S, Skuce CD, Smith ML, Soderlund C,
            Steward CA, Sulston JE, Swann M, Sycamore N, Taylor R, Tee L,
            Thomas DW, Thorpe A, Tracey A, Tromans AC, Vaudin M, Wall M, Wallis
            JM, Whitehead SL, Whittaker P, Willey DL, Williams L, Williams SA,
            Wilming L, Wray PW, Hubbard T, Durbin RM, Bentley DR, Beck S and
            Rogers J.
  TITLE     The DNA sequence and comparative analysis of human chromosome 20
  JOURNAL   Nature 414 (6866), 865-871 (2001)
   PUBMED   11780052
REFERENCE   9  (bases 1 to 1009)
  AUTHORS   Horikoshi N, Cong J, Kley N and Shenk T.
  TITLE     Isolation of differentially expressed cDNAs from p53-dependent
            apoptotic cells: activation of the human homologue of the
            Drosophila peroxidasin gene
  JOURNAL   Biochem Biophys Res Commun 261 (3), 864-869 (1999)
   PUBMED   10441517
REFERENCE   10 (bases 1 to 1009)
  AUTHORS   Ranganathan S, Simpson KJ, Shaw DC and Nicholas KR.
  TITLE     The whey acidic protein family: a new signature motif and
            three-dimensional structure by comparative modeling
  JOURNAL   J Mol Graph Model 17 (2), 106-113 (1999)
   PUBMED   10680116
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from Z93016.2, AY358822.1 and
            BC039173.1.
            
            On Jun 2, 2019 this sequence version replaced NM_145652.3.
            
            Summary: This gene encodes a member of the WAP-type four-disulfide
            core (WFDC) domain family. Most WFDC proteins contain only one WFDC
            domain, and this encoded protein contains two WFDC domains. The
            WFDC domain, or WAP signature motif, contains eight cysteines
            forming four disulfide bonds at the core of the protein, and
            functions as a protease inhibitor. Most WFDC gene members are
            localized to chromosome 20q12-q13 in two clusters: centromeric and
            telomeric. This gene belongs to the centromeric cluster. [provided
            by RefSeq, Jul 2008].
            
            ##Evidence-Data-START##
            Transcript exon combination :: AY358822.1, BC039173.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2144333, SAMEA2145893
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000372789.6/ ENSP00000361875.4
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2                 Z93016.2           115957-115958       c
            3-36                AY358822.1         1-34
            37-1004             BC039173.1         1-968
            1005-1009           Z93016.2           110247-110251       c
FEATURES             Location/Qualifiers
     source          1..1009
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="20"
                     /map="20q13.12"
     gene            1..1009
                     /gene="WFDC5"
                     /gene_synonym="dJ211D12.5; PRG5; WAP1"
                     /note="WAP four-disulfide core domain 5"
                     /db_xref="GeneID:149708"
                     /db_xref="HGNC:HGNC:20477"
                     /db_xref="MIM:605161"
     exon            1..176
                     /gene="WFDC5"
                     /gene_synonym="dJ211D12.5; PRG5; WAP1"
                     /inference="alignment:Splign:2.1.0"
     CDS             92..463
                     /gene="WFDC5"
                     /gene_synonym="dJ211D12.5; PRG5; WAP1"
                     /note="protease inhibitor WAP1; WAP four-disulfide core
                     domain protein 5; p53-responsive gene 5 protein; putative
                     protease inhibitor WAP1"
                     /codon_start=1
                     /product="WAP four-disulfide core domain protein 5
                     precursor"
                     /protein_id="NP_663627.1"
                     /db_xref="CCDS:CCDS33475.1"
                     /db_xref="GeneID:149708"
                     /db_xref="HGNC:HGNC:20477"
                     /db_xref="MIM:605161"
                     /translation="MRTQSLLLLGALLAVGSQLPAVFGRKKGEKSGGCPPDDGPCLLS
                     VPDQCVEDSQCPLTRKCCYRACFRQCVPRVSVKLGSCPEDQLRCLSPMNHLCHKDSDC
                     SGKKRCCHSACGRDCRDPARG"
     exon            177..317
                     /gene="WFDC5"
                     /gene_synonym="dJ211D12.5; PRG5; WAP1"
                     /inference="alignment:Splign:2.1.0"
     exon            318..458
                     /gene="WFDC5"
                     /gene_synonym="dJ211D12.5; PRG5; WAP1"
                     /inference="alignment:Splign:2.1.0"
     exon            459..1009
                     /gene="WFDC5"
                     /gene_synonym="dJ211D12.5; PRG5; WAP1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      987..992
                     /regulatory_class="polyA_signal_sequence"
                     /gene="WFDC5"
                     /gene_synonym="dJ211D12.5; PRG5; WAP1"
                     /note="hexamer: AATAAA"
     polyA_site      1009
                     /gene="WFDC5"
                     /gene_synonym="dJ211D12.5; PRG5; WAP1"
                     /note="major polyA site"
ORIGIN      
        1 ctctttcctc tcctgactaa gtttctctgg cttccctgag gctgcaggtg ttaatctggg
       61 gggccctggg ccctgagccg gcagcagaaa tatgaggacc cagagccttc tcctcctggg
      121 ggccctcctg gctgtgggga gtcagctgcc tgctgtcttt ggcaggaaga agggagagaa
      181 atcggggggc tgcccgccag atgatgggcc ctgcctccta tcggtgcctg accagtgcgt
      241 ggaagacagc cagtgtccct tgaccaggaa gtgctgctac agagcttgct tccgccagtg
      301 tgtccccagg gtctctgtga agctgggcag ctgcccagag gaccaactgc gctgcctcag
      361 ccccatgaac cacctgtgtc acaaggactc agactgctcg ggcaaaaagc gatgctgcca
      421 cagcgcctgc gggcgggatt gccgggatcc tgccagaggc taattctgat ttaggatctg
      481 tggctctgca cctaagctgg ggaccaacgg aaagagttca cgatgggagg cctggggccc
      541 tgcccgctgg acagcactat ctctaccagc ggtggttcca gccttctgat aatcactggc
      601 ctgctgacac ttccctgcaa cccatccacc cctggtttct cctcctggga gtcaaagtcc
      661 atagcctgag ctcggaggaa ggcctctgta tcaccccagt actctgcacc actgccatac
      721 gagcttccca cccttcctaa cgctttcaca ccaatccgta catgctgctt cctccaccaa
      781 aaatgcccaa ttcaggcaga ccctgacctc tccctcaggc agcccaacca tccagaatga
      841 atattcttgc agagttttcc aaacatcagt cattcacctc tttcatgatt ttcaccatac
      901 ctacaaaata gcaccatgat aggttgcacg ctgcctgtac caccatttac ttaatgtttt
      961 ctttaaatgg ctcacttttg tatataaata aattcatttc aaaagaaaa
//
