LOCUS       NM_080614                970 bp    mRNA    linear   PRI 01-JUN-2024
DEFINITION  Homo sapiens WAP four-disulfide core domain 3 (WFDC3), mRNA.
ACCESSION   NM_080614
VERSION     NM_080614.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 970)
  AUTHORS   Wu,W., Wang,J., Hu,Z., Zhao,Y., Wang,X., Bai,N., Chen,L. and Gao,P.
  TITLE     High WFDC3 gene expression is associated with poor prognosis and
            reduced immune cells infiltration in pancreatic adenocarcinoma: A
            study using the TCGA database and bioinformatics analysis
  JOURNAL   Medicine (Baltimore) 102 (42), e35595 (2023)
   PUBMED   37861515
  REMARK    GeneRIF: High WFDC3 gene expression is associated with poor
            prognosis and reduced immune cells infiltration in pancreatic
            adenocarcinoma: A study using the TCGA database and bioinformatics
            analysis.
REFERENCE   2  (bases 1 to 970)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   3  (bases 1 to 970)
  AUTHORS   Ferreira,Z., Seixas,S., Andres,A.M., Kretzschmar,W.W.,
            Mullikin,J.C., Cherukuri,P.F., Cruz,P., Swanson,W.J., Clark,A.G.,
            Green,E.D. and Hurle,B.
  CONSRTM   NISC Comparative Sequencing Program
  TITLE     Reproduction and immunity-driven natural selection in the human
            WFDC locus
  JOURNAL   Mol Biol Evol 30 (4), 938-950 (2013)
   PUBMED   23292442
REFERENCE   4  (bases 1 to 970)
  AUTHORS   Clauss,A., Lilja,H. and Lundwall,A.
  TITLE     The evolution of a genetic locus encoding small serine proteinase
            inhibitors
  JOURNAL   Biochem Biophys Res Commun 333 (2), 383-389 (2005)
   PUBMED   15950183
REFERENCE   5  (bases 1 to 970)
  AUTHORS   Clauss,A., Lilja,H. and Lundwall,A.
  TITLE     A locus on human chromosome 20 contains several genes expressing
            protease inhibitor domains with homology to whey acidic protein
  JOURNAL   Biochem J 368 (Pt 1), 233-242 (2002)
   PUBMED   12206714
REFERENCE   6  (bases 1 to 970)
  AUTHORS   Deloukas,P., Matthews,L.H., Ashurst,J., Burton,J., Gilbert,J.G.,
            Jones,M., Stavrides,G., Almeida,J.P., Babbage,A.K., Bagguley,C.L.,
            Bailey,J., Barlow,K.F., Bates,K.N., Beard,L.M., Beare,D.M.,
            Beasley,O.P., Bird,C.P., Blakey,S.E., Bridgeman,A.M., Brown,A.J.,
            Buck,D., Burrill,W., Butler,A.P., Carder,C., Carter,N.P.,
            Chapman,J.C., Clamp,M., Clark,G., Clark,L.N., Clark,S.Y.,
            Clee,C.M., Clegg,S., Cobley,V.E., Collier,R.E., Connor,R.,
            Corby,N.R., Coulson,A., Coville,G.J., Deadman,R., Dhami,P.,
            Dunn,M., Ellington,A.G., Frankland,J.A., Fraser,A., French,L.,
            Garner,P., Grafham,D.V., Griffiths,C., Griffiths,M.N., Gwilliam,R.,
            Hall,R.E., Hammond,S., Harley,J.L., Heath,P.D., Ho,S., Holden,J.L.,
            Howden,P.J., Huckle,E., Hunt,A.R., Hunt,S.E., Jekosch,K.,
            Johnson,C.M., Johnson,D., Kay,M.P., Kimberley,A.M., King,A.,
            Knights,A., Laird,G.K., Lawlor,S., Lehvaslaiho,M.H., Leversha,M.,
            Lloyd,C., Lloyd,D.M., Lovell,J.D., Marsh,V.L., Martin,S.L.,
            McConnachie,L.J., McLay,K., McMurray,A.A., Milne,S., Mistry,D.,
            Moore,M.J., Mullikin,J.C., Nickerson,T., Oliver,K., Parker,A.,
            Patel,R., Pearce,T.A., Peck,A.I., Phillimore,B.J.,
            Prathalingam,S.R., Plumb,R.W., Ramsay,H., Rice,C.M., Ross,M.T.,
            Scott,C.E., Sehra,H.K., Shownkeen,R., Sims,S., Skuce,C.D.,
            Smith,M.L., Soderlund,C., Steward,C.A., Sulston,J.E., Swann,M.,
            Sycamore,N., Taylor,R., Tee,L., Thomas,D.W., Thorpe,A., Tracey,A.,
            Tromans,A.C., Vaudin,M., Wall,M., Wallis,J.M., Whitehead,S.L.,
            Whittaker,P., Willey,D.L., Williams,L., Williams,S.A., Wilming,L.,
            Wray,P.W., Hubbard,T., Durbin,R.M., Bentley,D.R., Beck,S. and
            Rogers,J.
  TITLE     The DNA sequence and comparative analysis of human chromosome 20
  JOURNAL   Nature 414 (6866), 865-871 (2001)
   PUBMED   11780052
REFERENCE   7  (bases 1 to 970)
  AUTHORS   Ranganathan,S., Simpson,K.J., Shaw,D.C. and Nicholas,K.R.
  TITLE     The whey acidic protein family: a new signature motif and
            three-dimensional structure by comparative modeling
  JOURNAL   J Mol Graph Model 17 (2), 106-113 (1999)
   PUBMED   10680116
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL050348.22.
            
            On Nov 26, 2018 this sequence version replaced NM_080614.1.
            
            Summary: This gene encodes a member of the WAP-type four-disulfide
            core (WFDC) domain family. The WFDC domain, or WAP signature motif,
            contains eight cysteines forming four disulfide bonds at the core
            of the protein, and functions as a protease inhibitor. The encoded
            protein contains four WFDC domains. Most WFDC genes are localized
            to chromosome 20q12-q13 in two clusters: centromeric and telomeric.
            This gene belongs to the telomeric cluster. Alternatively spliced
            transcript variants have been observed but their full-length nature
            has not been determined. [provided by RefSeq, Jul 2008].
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF488306.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968968 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000243938.9/ ENSP00000243938.4
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-52                AL050348.22        61491-61542         c
            53-141              AL050348.22        59553-59641         c
            142-270             AL050348.22        58590-58718         c
            271-417             AL050348.22        57495-57641         c
            418-552             AL050348.22        46734-46868         c
            553-738             AL050348.22        45076-45261         c
            739-970             AL050348.22        43872-44103         c
FEATURES             Location/Qualifiers
     source          1..970
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="20"
                     /map="20q13.12"
     gene            1..970
                     /gene="WFDC3"
                     /gene_synonym="dJ447F3.3; WAP14"
                     /note="WAP four-disulfide core domain 3"
                     /db_xref="GeneID:140686"
                     /db_xref="HGNC:HGNC:15957"
     exon            1..52
                     /gene="WFDC3"
                     /gene_synonym="dJ447F3.3; WAP14"
                     /inference="alignment:Splign:2.1.0"
     exon            53..141
                     /gene="WFDC3"
                     /gene_synonym="dJ447F3.3; WAP14"
                     /inference="alignment:Splign:2.1.0"
     CDS             60..755
                     /gene="WFDC3"
                     /gene_synonym="dJ447F3.3; WAP14"
                     /note="protease inhibitor WAP14; whey acidic protein 14;
                     WAP four-disulfide core domain protein 3; putative
                     protease inhibitor WAP14"
                     /codon_start=1
                     /product="WAP four-disulfide core domain protein 3
                     precursor"
                     /protein_id="NP_542181.1"
                     /db_xref="CCDS:CCDS33478.1"
                     /db_xref="GeneID:140686"
                     /db_xref="HGNC:HGNC:15957"
                     /translation="MMLSCLFLLKALLALGSLESWITAGEHAKEGECPPHKNPCKELC
                     QGDELCPAEQKCCTTGCGRICRDIPKGRKRDCPRVIRKQSCLKRCITDETCPGVKKCC
                     TLGCNKSCVVPISKQKLAEFGGECPADPLPCEELCDGDASCPQGHKCCSTGCGRTCLG
                     DIEGGRGGDCPKVLVGLCIVGCVMDENCQAGEKCCKSGCGRFCVPPVLPPKLTMNPNW
                     TVRSDSELEIPVP"
     exon            142..270
                     /gene="WFDC3"
                     /gene_synonym="dJ447F3.3; WAP14"
                     /inference="alignment:Splign:2.1.0"
     exon            271..417
                     /gene="WFDC3"
                     /gene_synonym="dJ447F3.3; WAP14"
                     /inference="alignment:Splign:2.1.0"
     exon            418..552
                     /gene="WFDC3"
                     /gene_synonym="dJ447F3.3; WAP14"
                     /inference="alignment:Splign:2.1.0"
     exon            553..738
                     /gene="WFDC3"
                     /gene_synonym="dJ447F3.3; WAP14"
                     /inference="alignment:Splign:2.1.0"
     exon            739..970
                     /gene="WFDC3"
                     /gene_synonym="dJ447F3.3; WAP14"
                     /inference="alignment:Splign:2.1.0"
     regulatory      953..958
                     /regulatory_class="polyA_signal_sequence"
                     /gene="WFDC3"
                     /gene_synonym="dJ447F3.3; WAP14"
                     /note="hexamer: GATAAA"
     polyA_site      970
                     /gene="WFDC3"
                     /gene_synonym="dJ447F3.3; WAP14"
                     /note="major polyA site"
ORIGIN      
        1 atgggaaccg aaccgccgcg cctcgcccag gacagttaca cttagaggcc ttcatcatca
       61 tgatgttaag ctgcctcttt cttctgaagg cacttcttgc tcttgggtct ctggaatcct
      121 ggataactgc aggagaacat gcaaaagagg gagaatgccc tccccataag aacccatgca
      181 aagagctgtg ccagggtgat gaattgtgtc cggctgaaca gaagtgctgc accacaggct
      241 gtggtcggat ctgccgagac attcctaagg ggaggaaaag agattgccct agggttattc
      301 ggaaacaatc ctgtttgaaa aggtgcatca ctgatgagac atgtccaggt gtaaagaaat
      361 gctgcacgct tggctgcaac aagagctgtg tagtcccaat ctctaaacag aagctggcag
      421 agtttggtgg tgaatgtccc gctgaccccc ttccgtgtga ggagctgtgt gatggggatg
      481 catcctgtcc ccaggggcat aaatgctgca gcaccggctg tggccgcacc tgcctcggag
      541 acattgaggg agggcggggc ggtgattgtc caaaagttct ggtgggcctg tgcattgttg
      601 gctgtgtgat ggatgagaat tgtcaagctg gagaaaaatg ttgcaagtca ggctgtggcc
      661 gcttctgtgt cccaccagtc ctgcccccaa aactgaccat gaaccccaac tggactgtga
      721 ggtctgattc cgaattagag atcccggtgc cctagctgtg ctgatttgtc tggagcttct
      781 ttggtaattc tggaagcttt tcctggcagt caagagaggg tgacatcctg gggcttgtga
      841 catttccagg ggcactcatg gccctctctg ctctgcttct cctcctgccg ctgaccagag
      901 catgggaaat agccctggat tgggtagtgg gtgtgtggtg cttctctttc ccgataaagg
      961 ctggtgctga
//
