LOCUS       NM_172006                632 bp    mRNA    linear   PRI 01-JUN-2024
DEFINITION  Homo sapiens WAP four-disulfide core domain 10B (WFDC10B),
            transcript variant 1, mRNA.
ACCESSION   NM_172006
VERSION     NM_172006.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 632)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   2  (bases 1 to 632)
  AUTHORS   Ferreira,Z., Seixas,S., Andres,A.M., Kretzschmar,W.W.,
            Mullikin,J.C., Cherukuri,P.F., Cruz,P., Swanson,W.J., Clark,A.G.,
            Green,E.D. and Hurle,B.
  CONSRTM   NISC Comparative Sequencing Program
  TITLE     Reproduction and immunity-driven natural selection in the human
            WFDC locus
  JOURNAL   Mol Biol Evol 30 (4), 938-950 (2013)
   PUBMED   23292442
REFERENCE   3  (bases 1 to 632)
  AUTHORS   Clauss,A., Lilja,H. and Lundwall,A.
  TITLE     The evolution of a genetic locus encoding small serine proteinase
            inhibitors
  JOURNAL   Biochem Biophys Res Commun 333 (2), 383-389 (2005)
   PUBMED   15950183
REFERENCE   4  (bases 1 to 632)
  AUTHORS   Clauss,A., Lilja,H. and Lundwall,A.
  TITLE     A locus on human chromosome 20 contains several genes expressing
            protease inhibitor domains with homology to whey acidic protein
  JOURNAL   Biochem J 368 (Pt 1), 233-242 (2002)
   PUBMED   12206714
REFERENCE   5  (bases 1 to 632)
  AUTHORS   Deloukas,P., Matthews,L.H., Ashurst,J., Burton,J., Gilbert,J.G.,
            Jones,M., Stavrides,G., Almeida,J.P., Babbage,A.K., Bagguley,C.L.,
            Bailey,J., Barlow,K.F., Bates,K.N., Beard,L.M., Beare,D.M.,
            Beasley,O.P., Bird,C.P., Blakey,S.E., Bridgeman,A.M., Brown,A.J.,
            Buck,D., Burrill,W., Butler,A.P., Carder,C., Carter,N.P.,
            Chapman,J.C., Clamp,M., Clark,G., Clark,L.N., Clark,S.Y.,
            Clee,C.M., Clegg,S., Cobley,V.E., Collier,R.E., Connor,R.,
            Corby,N.R., Coulson,A., Coville,G.J., Deadman,R., Dhami,P.,
            Dunn,M., Ellington,A.G., Frankland,J.A., Fraser,A., French,L.,
            Garner,P., Grafham,D.V., Griffiths,C., Griffiths,M.N., Gwilliam,R.,
            Hall,R.E., Hammond,S., Harley,J.L., Heath,P.D., Ho,S., Holden,J.L.,
            Howden,P.J., Huckle,E., Hunt,A.R., Hunt,S.E., Jekosch,K.,
            Johnson,C.M., Johnson,D., Kay,M.P., Kimberley,A.M., King,A.,
            Knights,A., Laird,G.K., Lawlor,S., Lehvaslaiho,M.H., Leversha,M.,
            Lloyd,C., Lloyd,D.M., Lovell,J.D., Marsh,V.L., Martin,S.L.,
            McConnachie,L.J., McLay,K., McMurray,A.A., Milne,S., Mistry,D.,
            Moore,M.J., Mullikin,J.C., Nickerson,T., Oliver,K., Parker,A.,
            Patel,R., Pearce,T.A., Peck,A.I., Phillimore,B.J.,
            Prathalingam,S.R., Plumb,R.W., Ramsay,H., Rice,C.M., Ross,M.T.,
            Scott,C.E., Sehra,H.K., Shownkeen,R., Sims,S., Skuce,C.D.,
            Smith,M.L., Soderlund,C., Steward,C.A., Sulston,J.E., Swann,M.,
            Sycamore,N., Taylor,R., Tee,L., Thomas,D.W., Thorpe,A., Tracey,A.,
            Tromans,A.C., Vaudin,M., Wall,M., Wallis,J.M., Whitehead,S.L.,
            Whittaker,P., Willey,D.L., Williams,L., Williams,S.A., Wilming,L.,
            Wray,P.W., Hubbard,T., Durbin,R.M., Bentley,D.R., Beck,S. and
            Rogers,J.
  TITLE     The DNA sequence and comparative analysis of human chromosome 20
  JOURNAL   Nature 414 (6866), 865-871 (2001)
   PUBMED   11780052
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL109656.12 and AL031671.12.
            
            On Jun 10, 2003 this sequence version replaced NM_172006.1.
            
            Summary: This gene encodes a member of the WAP-type four-disulfide
            core (WFDC) domain family. The WFDC domain, or WAP signature motif,
            contains eight cysteines forming four disulfide bonds at the core
            of the protein, and functions as a protease inhibitor. Most WFDC
            gene members are localized to chromosome 20q12-q13 in two clusters:
            centromeric and telomeric. This gene belongs to the telomeric
            cluster. Two alternatively spliced transcript variants have been
            found for this gene, and they encode distinct isoforms. [provided
            by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (1) represents the longer
            transcript but it encodes the shorter isoform (a), which includes a
            signal peptide and is a secreted protein.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF454506.1, BC121072.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968968 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000330523.10/ ENSP00000327628.5
            RefSeq Select criteria :: based on expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-102               AL109656.12        7964-8065           c
            103-167             AL109656.12        7543-7607           c
            168-322             AL031671.12        105228-105382       c
            323-632             AL031671.12        103977-104286       c
FEATURES             Location/Qualifiers
     source          1..632
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="20"
                     /map="20q13.12"
     gene            1..632
                     /gene="WFDC10B"
                     /gene_synonym="WAP12"
                     /note="WAP four-disulfide core domain 10B"
                     /db_xref="GeneID:280664"
                     /db_xref="HGNC:HGNC:20479"
     exon            1..102
                     /gene="WFDC10B"
                     /gene_synonym="WAP12"
                     /inference="alignment:Splign:2.1.0"
     exon            103..167
                     /gene="WFDC10B"
                     /gene_synonym="WAP12"
                     /inference="alignment:Splign:2.1.0"
     exon            168..322
                     /gene="WFDC10B"
                     /gene_synonym="WAP12"
                     /inference="alignment:Splign:2.1.0"
     CDS             232..453
                     /gene="WFDC10B"
                     /gene_synonym="WAP12"
                     /note="isoform a precursor is encoded by transcript
                     variant 1; protease inhibitor WAP12; protein WFDC10B"
                     /codon_start=1
                     /product="protein WFDC10B isoform a precursor"
                     /protein_id="NP_742003.1"
                     /db_xref="CCDS:CCDS13366.1"
                     /db_xref="GeneID:280664"
                     /db_xref="HGNC:HGNC:20479"
                     /translation="MAPQTLLLVLVLCVLLLQAQGGYRDKMRMQRIKVCEKRPSIDLC
                     IHHCSYFQKCETNKICCSAFCGNICMSIL"
     exon            323..632
                     /gene="WFDC10B"
                     /gene_synonym="WAP12"
                     /inference="alignment:Splign:2.1.0"
     regulatory      607..612
                     /regulatory_class="polyA_signal_sequence"
                     /gene="WFDC10B"
                     /gene_synonym="WAP12"
                     /note="hexamer: AATAAA"
     polyA_site      632
                     /gene="WFDC10B"
                     /gene_synonym="WAP12"
                     /note="major polyA site"
ORIGIN      
        1 agagcccaag aacactggtg gccaaaggat ggtgtcttgt gaaaggaggg caagcaaaag
       61 tgtaggacaa attttgcttt gggattttgg ttacacctgc agactatccc acagaaggag
      121 gaacagcact gaaatccttt ctcgcaatct tcctgcattg tacacaggca gctctgcagg
      181 gaagtctgtg actgcctggc cagacttagg gctcacgctc tggtcagagt tatggcaccc
      241 cagactctgc tgcttgtcct ggttctctgt gtgctgctgc tgcaggccca gggaggatac
      301 cgtgacaaga tgaggatgca gagaatcaag gtctgtgaga agcgacccag catagatcta
      361 tgcatccacc actgttcata tttccaaaag tgtgaaacaa ataagatatg ctgttcagcc
      421 ttctgtggga acatttgtat gagcatccta tgagtgggag agtgggctgg gatgtgcatc
      481 ctgctccctg aacccttcca tccgagactg tgcccacatc cgaagcacaa ggacatcaaa
      541 tcatcagcac aagaacatca acaggaatgc caccctcccc agtgtctgaa ctccctgtcc
      601 ctgtcaaata aaccagaaca aatgcccatg ga
//
