LOCUS       NM_021197               1295 bp    mRNA    linear   PRI 01-JUN-2024
DEFINITION  Homo sapiens WAP four-disulfide core domain 1 (WFDC1), transcript
            variant 1, mRNA.
ACCESSION   NM_021197
VERSION     NM_021197.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1295)
  AUTHORS   Zhu,S., Ye,L., Bennett,S., Xu,H., He,D. and Xu,J.
  TITLE     Molecular structure, gene expression and functional role of WFDC1
            in angiogenesis and cancer
  JOURNAL   Cell Biochem Funct 39 (5), 588-595 (2021)
   PUBMED   33615507
  REMARK    GeneRIF: Molecular structure, gene expression and functional role
            of WFDC1 in angiogenesis and cancer.
            Review article
REFERENCE   2  (bases 1 to 1295)
  AUTHORS   Solis-Calero,C. and Carvalho,H.F.
  TITLE     Phylogenetic, molecular evolution and structural analyses of the
            WFDC1/prostate stromal protein 20 (ps20)
  JOURNAL   Gene 686, 125-140 (2019)
   PUBMED   30423385
  REMARK    GeneRIF: Phylogenetic, molecular evolution and structural analyses
            of the WFDC1/prostate stromal protein 20.
REFERENCE   3  (bases 1 to 1295)
  AUTHORS   Hickman,O.J., Smith,R.A., Dasgupta,P., Rao,S.N., Nayak,S.,
            Sreenivasan,S., Vyakarnam,A. and Galustian,C.
  TITLE     Expression of two WFDC1/ps20 isoforms in prostate stromal cells
            induces paracrine apoptosis through regulation of PTGS2/COX-2
  JOURNAL   Br J Cancer 114 (11), 1235-1242 (2016)
   PUBMED   27115470
  REMARK    GeneRIF: Prostrate stromal 20 overexpression upregulates expression
            of cyclooxygenase-2 (COX-2) in LNCaP and WPMY-1 cells, and induces
            expression of a growth-suppressive phenotype, which inhibits
            proliferation of PCa cells by ps20-expressing WPMY-1 conditioned
            media.
REFERENCE   4  (bases 1 to 1295)
  AUTHORS   Simino,J., Sung,Y.J., Kume,R., Schwander,K. and Rao,D.C.
  TITLE     Gene-alcohol interactions identify several novel blood pressure
            loci including a promising locus near SLC16A9
  JOURNAL   Front Genet 4, 277 (2013)
   PUBMED   24376456
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 1295)
  AUTHORS   Song,H., Kwan,S.Y., Izaguirre,D.I., Zu,Z., Tsang,Y.T., Tung,C.S.,
            King,E.R., Mok,S.C., Gershenson,D.M. and Wong,K.K.
  TITLE     PAX2 Expression in Ovarian Cancer
  JOURNAL   Int J Mol Sci 14 (3), 6090-6105 (2013)
   PUBMED   23502471
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 1295)
  AUTHORS   Alvarez,R., Reading,J., King,D.F., Hayes,M., Easterbrook,P.,
            Farzaneh,F., Ressler,S., Yang,F., Rowley,D. and Vyakarnam,A.
  TITLE     WFDC1/ps20 is a novel innate immunomodulatory signature protein of
            human immunodeficiency virus (HIV)-permissive CD4+ CD45RO+ memory T
            cells that promotes infection by upregulating CD54 integrin
            expression and is elevated in HIV type 1 infection
  JOURNAL   J Virol 82 (1), 471-486 (2008)
   PUBMED   17942534
  REMARK    GeneRIF: ps20 promoted HIV entry via fusion and augmented CD54
            integrin expression
REFERENCE   7  (bases 1 to 1295)
  AUTHORS   Watson,J.E., Kamkar,S., James,K., Kowbel,D., Andaya,A., Paris,P.L.,
            Simko,J., Carroll,P., McAlhany,S., Rowley,D. and Collins,C.
  TITLE     Molecular analysis of WFDC1/ps20 gene in prostate cancer
  JOURNAL   Prostate 61 (2), 192-199 (2004)
   PUBMED   15305342
  REMARK    GeneRIF: WFDC1/ps20 is down regulated but not frequently mutated in
            prostate cancer. It is expressed predominantly in the normal
            prostatic stroma. It might play a role in the maintenance of the
            normal extra cellular matrix milieu in the prostate.
REFERENCE   8  (bases 1 to 1295)
  AUTHORS   McAlhany,S.J., Ressler,S.J., Larsen,M., Tuxhorn,J.A., Yang,F.,
            Dang,T.D. and Rowley,D.R.
  TITLE     Promotion of angiogenesis by ps20 in the differential reactive
            stroma prostate cancer xenograft model
  JOURNAL   Cancer Res 63 (18), 5859-5865 (2003)
   PUBMED   14522910
REFERENCE   9  (bases 1 to 1295)
  AUTHORS   Saffroy,R., Riou,P., Soler,G., Azoulay,D., Emile,J.F., Debuire,B.
            and Lemoine,A.
  TITLE     Analysis of alterations of WFDC1, a new putative tumour suppressor
            gene, in hepatocellular carcinoma
  JOURNAL   Eur J Hum Genet 10 (4), 239-244 (2002)
   PUBMED   12032731
  REMARK    GeneRIF: results suggest a minor role, if any, of WFDC1 in
            hepatocarcinogenesis
REFERENCE   10 (bases 1 to 1295)
  AUTHORS   Larsen,M., Ressler,S.J., Gerdes,M.J., Lu,B., Byron,M.,
            Lawrence,J.B. and Rowley,D.R.
  TITLE     The WFDC1 gene encoding ps20 localizes to 16q24, a region of LOH in
            multiple cancers
  JOURNAL   Mamm Genome 11 (9), 767-773 (2000)
   PUBMED   10967136
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from HY179606.1, BC029159.1 and
            W49668.1.
            
            On Nov 22, 2018 this sequence version replaced NM_021197.3.
            
            Summary: This gene encodes a member of the WAP-type four disulfide
            core domain family. The WAP-type four-disulfide core domain
            contains eight cysteines forming four disulfide bonds at the core
            of the protein, and functions as a protease inhibitor in many
            family members. This gene is mapped to chromosome 16q24, an area of
            frequent loss of heterozygosity in cancers, including prostate,
            breast and hepatocellular cancers and Wilms' tumor. This gene is
            downregulated in many cancer types and may be involved in the
            inhibition of cell proliferation. The encoded protein may also play
            a role in the susceptibility of certain CD4 memory T cells to human
            immunodeficiency virus infection. Alternative splicing results in
            multiple transcript variants. [provided by RefSeq, Sep 2013].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longer isoform (1). Both variants 1 and
            2 encode the same isoform.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF302109.1, AF169631.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968189, SAMEA1968968
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000219454.10/ ENSP00000219454.5
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-22                HY179606.1         173-194
            23-1039             BC029159.1         1-1017
            1040-1295           W49668.1           13-268              c
FEATURES             Location/Qualifiers
     source          1..1295
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="16"
                     /map="16q24.1"
     gene            1..1295
                     /gene="WFDC1"
                     /gene_synonym="PS20"
                     /note="WAP four-disulfide core domain 1"
                     /db_xref="GeneID:58189"
                     /db_xref="HGNC:HGNC:15466"
                     /db_xref="MIM:605322"
     exon            1..238
                     /gene="WFDC1"
                     /gene_synonym="PS20"
                     /inference="alignment:Splign:2.1.0"
     CDS             95..757
                     /gene="WFDC1"
                     /gene_synonym="PS20"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; prostate stromal protein ps20; ps20 growth
                     inhibitor; WAP four-disulfide core domain 1 homolog"
                     /codon_start=1
                     /product="WAP four-disulfide core domain protein 1 isoform
                     1 precursor"
                     /protein_id="NP_067020.2"
                     /db_xref="CCDS:CCDS10946.1"
                     /db_xref="GeneID:58189"
                     /db_xref="HGNC:HGNC:15466"
                     /db_xref="MIM:605322"
                     /translation="MPLTGVGPGSCRRQIIRALCLLLLLLHAGSAKNIWKRALPARLA
                     EKSRAEEAGAPGGPRQPRADRCPPPPRTLPPGACQAARCQADSECPRHRRCCYNGCAY
                     ACLEAVPPPPVLDWLVQPKPRWLGGNGWLLDGPEEVLQAEACSTTEDGAEPLLCPSGY
                     ECHILSPGDVAEGIPNRGQCVKQRRQADGRILRHKLYKEYPEGDSKNVAEPGRGQQKH
                     FQ"
     exon            239..431
                     /gene="WFDC1"
                     /gene_synonym="PS20"
                     /inference="alignment:Splign:2.1.0"
     exon            432..515
                     /gene="WFDC1"
                     /gene_synonym="PS20"
                     /inference="alignment:Splign:2.1.0"
     exon            516..656
                     /gene="WFDC1"
                     /gene_synonym="PS20"
                     /inference="alignment:Splign:2.1.0"
     exon            657..698
                     /gene="WFDC1"
                     /gene_synonym="PS20"
                     /inference="alignment:Splign:2.1.0"
     exon            699..772
                     /gene="WFDC1"
                     /gene_synonym="PS20"
                     /inference="alignment:Splign:2.1.0"
     exon            773..1295
                     /gene="WFDC1"
                     /gene_synonym="PS20"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1275..1280
                     /regulatory_class="polyA_signal_sequence"
                     /gene="WFDC1"
                     /gene_synonym="PS20"
                     /note="hexamer: AATAAA"
     polyA_site      1295
                     /gene="WFDC1"
                     /gene_synonym="PS20"
                     /note="major polyA site"
ORIGIN      
        1 agactgtgca cgctcctgtc cccactcaca ggcccacgca gcgagggggg cccctcttct
       61 gtgtgcgtct ggaaggtcgc tgcccaggga ggaaatgcct ttaaccggcg tggggccggg
      121 cagctgcagg aggcagatca tccgggctct gtgcctcttg ctacttctcc tccacgccgg
      181 ctctgccaag aatatctgga aacgggcatt gcctgcgagg ctggccgaga aatcccgtgc
      241 cgaggaggcg ggcgcgcccg gcggcccccg gcagccccga gcagaccgct gcccgccgcc
      301 tccgcggacg ctgccccccg gcgcctgcca ggccgcgcgc tgtcaggcgg actccgagtg
      361 cccgcggcac cggcgctgct gctacaacgg atgcgcctac gcctgcctag aagctgtgcc
      421 gcccccgcca gtcttagact ggctggtgca gccgaaacct cgatggcttg gtggcaatgg
      481 ctggctcctg gatggccctg aggaggtgtt acaagcagag gcgtgcagca ccacggagga
      541 tggggccgaa cccctgctct gtccctcggg ctatgagtgc cacatcctga gcccaggtga
      601 cgtggccgaa ggtatcccca accgtgggca gtgcgtcaag cagcgccggc aagcagatgg
      661 gcgaatccta cgacacaaac tttacaaaga atatccagaa ggtgactcaa agaatgtggc
      721 agaacctgga aggggacaac agaagcactt tcagtaaagc aacggcaagc agctaggttg
      781 caagaacatt cctctacttt ctgctaagcc ttggaaacag ttgggaaaag tagtttgacc
      841 ctcacagttc acattcagct cagcagagca agaccccaga gatgcttaga gacaggacac
      901 ctggccatca aacccagttt ggcccagcct ggttgggtga ctttgtggga gccacttaac
      961 agctctgggt ccctgtttta ccatcctggg agcaaggccc tgcagctcca cgagaccttt
     1021 accccgggaa gaagccgcca cccatgaaag catttctgaa gcccctttct aagacaaggc
     1081 tcagcatctt gatatttttg acagattcct cccaagtctg gctctgggag gtatgtaccc
     1141 atctcaaatg ttcccaagat aaattcatcc ttcaggaaat ggaaatgaac ttgcttacta
     1201 atgtgtgatt cctagttgta gccaccggat gtgctgaggc ctaaatgtta gcaggtggga
     1261 ggaggccaca gaacaataaa aacaaccaaa taaga
//
