LOCUS       NM_003390               3588 bp    mRNA    linear   PRI 11-SEP-2024
DEFINITION  Homo sapiens WEE1 G2 checkpoint kinase (WEE1), transcript variant
            1, mRNA.
ACCESSION   NM_003390
VERSION     NM_003390.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3588)
  AUTHORS   Zhang,L., He,S., Guan,H., Zhao,Y. and Zhang,D.
  TITLE     Circulating RNA ZFR promotes hepatocellular carcinoma cell
            proliferation and epithelial-mesenchymal transition process through
            miR-624-3p/WEE1 axis
  JOURNAL   Hepatobiliary Pancreat Dis Int 23 (1), 52-63 (2024)
   PUBMED   37516591
  REMARK    GeneRIF: Circulating RNA ZFR promotes hepatocellular carcinoma cell
            proliferation and epithelial-mesenchymal transition process through
            miR-624-3p/WEE1 axis.
REFERENCE   2  (bases 1 to 3588)
  AUTHORS   Colaco,V., Goswami,N., Goel,V.K., Srivastava,S.K., Lalrohlua,P.,
            Senthil Kumar,N., Borah,P., Baruah,R. and Varma,A.K.
  TITLE     In silico and structure-based evaluation of deleterious mutations
            identified in human Chk1, Chk2, and Wee1 protein kinase
  JOURNAL   J Cell Biochem 125 (1), 89-99 (2024)
   PUBMED   38047473
  REMARK    GeneRIF: In silico and structure-based evaluation of deleterious
            mutations identified in human Chk1, Chk2, and Wee1 protein kinase.
REFERENCE   3  (bases 1 to 3588)
  AUTHORS   Zhong,W.J., Zhang,L.Z., Yue,F., Yuan,L., Zhang,Q., Li,X. and Lin,L.
  TITLE     Identification of DNA methylation-regulated WEE1 with potential
            implications in prognosis and immunotherapy for low-grade glioma
  JOURNAL   Cancer Biomark 40 (3-4), 297-317 (2024)
   PUBMED   39213054
  REMARK    GeneRIF: Identification of DNA methylation-regulated WEE1 with
            potential implications in prognosis and immunotherapy for low-grade
            glioma.
REFERENCE   4  (bases 1 to 3588)
  AUTHORS   Liu,K., Wang,L., Lou,Z., Guo,L., Xu,Y., Qi,H., Fang,Z., Mei,L.,
            Chen,X., Zhang,X., Shao,J. and Xiang,X.
  TITLE     E2F8 exerts cancer-promoting effects by transcriptionally
            activating RRM2 and E2F8 knockdown synergizes with WEE1 inhibition
            in suppressing lung adenocarcinoma
  JOURNAL   Biochem Pharmacol 218, 115854 (2023)
   PUBMED   37863324
  REMARK    GeneRIF: E2F8 exerts cancer-promoting effects by transcriptionally
            activating RRM2 and E2F8 knockdown synergizes with WEE1 inhibition
            in suppressing lung adenocarcinoma.
REFERENCE   5  (bases 1 to 3588)
  AUTHORS   Segal,D., Maier,S., Mastromarco,G.J., Qian,W.W., Nabeel-Shah,S.,
            Lee,H., Moore,G., Lacoste,J., Larsen,B., Lin,Z.Y.,
            Selvabaskaran,A., Liu,K., Smibert,C., Zhang,Z., Greenblatt,J.,
            Peng,J., Lee,H.O., Gingras,A.C. and Taipale,M.
  TITLE     A central chaperone-like role for 14-3-3 proteins in human cells
  JOURNAL   Mol Cell 83 (6), 974-993 (2023)
   PUBMED   36931259
REFERENCE   6  (bases 1 to 3588)
  AUTHORS   Baldin,V. and Ducommun,B.
  TITLE     Subcellular localisation of human wee1 kinase is regulated during
            the cell cycle
  JOURNAL   J Cell Sci 108 (Pt 6), 2425-2432 (1995)
   PUBMED   7673359
REFERENCE   7  (bases 1 to 3588)
  AUTHORS   Watanabe,N., Broome,M. and Hunter,T.
  TITLE     Regulation of the human WEE1Hu CDK tyrosine 15-kinase during the
            cell cycle
  JOURNAL   EMBO J 14 (9), 1878-1891 (1995)
   PUBMED   7743995
REFERENCE   8  (bases 1 to 3588)
  AUTHORS   Gu,Y., Rosenblatt,J. and Morgan,D.O.
  TITLE     Cell cycle regulation of CDK2 activity by phosphorylation of Thr160
            and Tyr15
  JOURNAL   EMBO J 11 (11), 3995-4005 (1992)
   PUBMED   1396589
REFERENCE   9  (bases 1 to 3588)
  AUTHORS   Parker,L.L. and Piwnica-Worms,H.
  TITLE     Inactivation of the p34cdc2-cyclin B complex by the human WEE1
            tyrosine kinase
  JOURNAL   Science 257 (5078), 1955-1957 (1992)
   PUBMED   1384126
REFERENCE   10 (bases 1 to 3588)
  AUTHORS   Igarashi,M., Nagata,A., Jinno,S., Suto,K. and Okayama,H.
  TITLE     Wee1(+)-like gene in human cells
  JOURNAL   Nature 353 (6339), 80-83 (1991)
   PUBMED   1840647
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC122179.6, U10564.1,
            BC051831.1, BQ024511.1 and AC011979.7.
            
            On Nov 22, 2018 this sequence version replaced NM_003390.3.
            
            Summary: This gene encodes a nuclear protein, which is a tyrosine
            kinase belonging to the Ser/Thr family of protein kinases. This
            protein catalyzes the inhibitory tyrosine phosphorylation of
            CDC2/cyclin B kinase, and appears to coordinate the transition
            between DNA replication and mitosis by protecting the nucleus from
            cytoplasmically activated CDC2 kinase. [provided by RefSeq, Jul
            2008].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC051831.1, X62048.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000450114.7/ ENSP00000402084.2
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-11                AC122179.6         43676-43686
            12-2205             U10564.1           1-2194
            2206-3365           BC051831.1         1993-3152
            3366-3370           BQ024511.1         16-20               c
            3371-3588           AC011979.7         136521-136738       c
FEATURES             Location/Qualifiers
     source          1..3588
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="11"
                     /map="11p15.4"
     gene            1..3588
                     /gene="WEE1"
                     /gene_synonym="WEE1A; WEE1hu"
                     /note="WEE1 G2 checkpoint kinase"
                     /db_xref="GeneID:7465"
                     /db_xref="HGNC:HGNC:12761"
                     /db_xref="MIM:193525"
     exon            1..840
                     /gene="WEE1"
                     /gene_synonym="WEE1A; WEE1hu"
                     /inference="alignment:Splign:2.1.0"
     CDS             265..2205
                     /gene="WEE1"
                     /gene_synonym="WEE1A; WEE1hu"
                     /EC_number="2.7.10.2"
                     /note="isoform 1 is encoded by transcript variant 1;
                     wee1-like protein kinase; wee1A kinase; WEE1+ homolog;
                     WEE1 homolog"
                     /codon_start=1
                     /product="wee1-like protein kinase isoform 1"
                     /protein_id="NP_003381.1"
                     /db_xref="CCDS:CCDS7800.1"
                     /db_xref="GeneID:7465"
                     /db_xref="HGNC:HGNC:12761"
                     /db_xref="MIM:193525"
                     /translation="MSFLSRQQPPPPRRAGAACTLRQKLIFSPCSDCEEEEEEEEEEG
                     SGHSTGEDSAFQEPDSPLPPARSPTEPGPERRRSPGPAPGSPGELEEDLLLPGACPGA
                     DEAGGGAEGDSWEEEGFGSSSPVKSPAAPYFLGSSFSPVRCGGPGDASPRGCGARRAG
                     EGRRSPRPDHPGTPPHKTFRKLRLFDTPHTPKSLLSKARGIDSSSVKLRGSSLFMDTE
                     KSGKREFDVRQTPQVNINPFTPDSLLLHSSGQCRRRKRTYWNDSCGEDMEASDYELED
                     ETRPAKRITITESNMKSRYTTEFHELEKIGSGEFGSVFKCVKRLDGCIYAIKRSKKPL
                     AGSVDEQNALREVYAHAVLGQHSHVVRYFSAWAEDDHMLIQNEYCNGGSLADAISENY
                     RIMSYFKEAELKDLLLQVGRGLRYIHSMSLVHMDIKPSNIFISRTSIPNAASEEGDED
                     DWASNKVMFKIGDLGHVTRISSPQVEEGDSRFLANEVLQENYTHLPKADIFALALTVV
                     CAAGAEPLPRNGDQWHEIRQGRLPRIPQVLSQEFTELLKVMIHPDPERRPSAMALVKH
                     SVLLSASRKSAEQLRIELNAEKFKNSLLQKELKKAQMAKAAAEERALFTDRMATRSTT
                     QSNRTSRLIGKKMNRSVSLTIY"
     exon            841..1046
                     /gene="WEE1"
                     /gene_synonym="WEE1A; WEE1hu"
                     /inference="alignment:Splign:2.1.0"
     exon            1047..1110
                     /gene="WEE1"
                     /gene_synonym="WEE1A; WEE1hu"
                     /inference="alignment:Splign:2.1.0"
     exon            1111..1283
                     /gene="WEE1"
                     /gene_synonym="WEE1A; WEE1hu"
                     /inference="alignment:Splign:2.1.0"
     exon            1284..1405
                     /gene="WEE1"
                     /gene_synonym="WEE1A; WEE1hu"
                     /inference="alignment:Splign:2.1.0"
     exon            1406..1552
                     /gene="WEE1"
                     /gene_synonym="WEE1A; WEE1hu"
                     /inference="alignment:Splign:2.1.0"
     exon            1553..1648
                     /gene="WEE1"
                     /gene_synonym="WEE1A; WEE1hu"
                     /inference="alignment:Splign:2.1.0"
     exon            1649..1734
                     /gene="WEE1"
                     /gene_synonym="WEE1A; WEE1hu"
                     /inference="alignment:Splign:2.1.0"
     exon            1735..1905
                     /gene="WEE1"
                     /gene_synonym="WEE1A; WEE1hu"
                     /inference="alignment:Splign:2.1.0"
     exon            1906..2051
                     /gene="WEE1"
                     /gene_synonym="WEE1A; WEE1hu"
                     /inference="alignment:Splign:2.1.0"
     exon            2052..3588
                     /gene="WEE1"
                     /gene_synonym="WEE1A; WEE1hu"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3334..3339
                     /regulatory_class="polyA_signal_sequence"
                     /gene="WEE1"
                     /gene_synonym="WEE1A; WEE1hu"
                     /note="hexamer: ATTAAA"
     polyA_site      3355
                     /gene="WEE1"
                     /gene_synonym="WEE1A; WEE1hu"
                     /note="major polyA site"
     regulatory      3565..3570
                     /regulatory_class="polyA_signal_sequence"
                     /gene="WEE1"
                     /gene_synonym="WEE1A; WEE1hu"
                     /note="hexamer: AATATA"
     polyA_site      3588
                     /gene="WEE1"
                     /gene_synonym="WEE1A; WEE1hu"
ORIGIN      
        1 ggagcccgaa cctgagactg gacctgagga gacctcagcc tcggtgctcg ggccgccccg
       61 cctctgccgg aaagtccgcg ccgccgctgc cgccaccgtc cgcagcccga gcgccccgga
      121 gccgcaggcc gccgccgcgc agagacgccg cggctgcgac taggcgcgcc cagccgcacg
      181 tggcggaccc gcccccaggc ccgcagtgtc ctggaccccg caggcctccg ctctcctgtc
      241 ctcggccccg tccccagggc cgcgatgagc ttcctgagcc gacagcagcc gccgccaccc
      301 cgccgcgccg gggcggcctg caccttgcgg cagaagctga tcttctcgcc ctgcagcgac
      361 tgtgaggagg aggaagaaga ggaggaggag gagggcagcg gccacagcac cggggaggac
      421 tcggcctttc aagagcccga ctcgccgctg ccgcccgcgc ggagccccac ggagcccggg
      481 cccgagcgcc gccgctcgcc cgggccggcc cccggcagcc ccggcgagct ggaggaggac
      541 ctgttgctgc ccggcgcctg cccgggcgcg gacgaggcgg gcggtggggc ggagggcgac
      601 tcgtgggagg aggagggctt cggctcctcg tcgccggtca agtcgccggc ggccccctac
      661 ttcctgggta gctctttctc gccggtgcgc tgcggcggcc caggagatgc gtcgccgcgg
      721 ggttgcgggg cgcgccgggc gggcgaaggc cgccgctcgc cgcggccgga ccacccgggc
      781 accccgccac acaagacctt ccgcaagctg cgactcttcg acaccccgca cacgcccaag
      841 agtttgctct ccaaagctcg gggaattgat tccagctctg ttaaactccg gggtagttct
      901 ctcttcatgg atacagaaaa atcaggaaaa agggaatttg atgtgcgaca gactcctcaa
      961 gtgaatatta atccttttac tccggattct ttgttgcttc attcctcagg acagtgtcgt
     1021 cgtagaaaga gaacgtattg gaatgattcc tgtggtgaag acatggaagc cagtgattat
     1081 gagcttgaag atgaaacaag acctgctaag agaattacaa ttactgaaag caatatgaag
     1141 tcccggtata caacagaatt tcatgagcta gagaaaatcg gctctggaga atttggttct
     1201 gtatttaagt gtgtgaagag gctggatgga tgcatttatg ccattaagcg atcaaaaaag
     1261 ccattggcgg gctctgttga tgagcagaac gctttgagag aagtatatgc tcatgcagtg
     1321 cttggacagc attctcatgt agttcgatat ttctctgcgt gggcagaaga tgatcatatg
     1381 cttatacaga atgaatattg taatggtgga agtttagctg atgctataag tgaaaactac
     1441 agaatcatga gttactttaa agaagcagag ttgaaggatc tccttttgca agttggccga
     1501 ggcttgaggt atattcattc aatgtctttg gttcacatgg atataaaacc tagtaatatt
     1561 ttcatatctc gaacctcaat cccaaatgct gcctctgaag aaggagacga agatgattgg
     1621 gcatccaaca aagttatgtt taaaataggt gatcttgggc atgtaacaag gatctccagt
     1681 ccacaagttg aagagggcga tagtcgtttt cttgcaaatg aagttttaca ggagaattat
     1741 acccatctac caaaagcaga tatttttgcg cttgccctca cagtggtatg tgctgctggt
     1801 gctgaacctc ttccgagaaa tggagatcaa tggcatgaaa tcagacaggg tagattacct
     1861 cggataccac aagtgctttc ccaagaattt acagagttgc taaaagttat gattcatcca
     1921 gatccagaga gaagaccttc agcaatggca ctggtaaagc attcagtatt gctgtccgct
     1981 tctagaaaga gtgcagaaca attacgaata gaattgaatg ccgaaaagtt caaaaattca
     2041 cttttacaaa aagaactcaa gaaagcacag atggcaaaag ctgcagctga ggaaagagca
     2101 ctcttcactg accggatggc cactaggtcc accacccaga gtaatagaac atctcgactt
     2161 attggaaaga aaatgaaccg ctctgtcagc cttactatat actgagctac tcctttccca
     2221 cctccccctg aacactgtga caagaggaag ctaggttgaa atcactgata gaatccagtt
     2281 tgcaattact ttctcgattg gtgtcagtag ttttactgat taggactttt attgtgaatt
     2341 acagttgaaa gctgtatttt gatgattgct atgtcaggct ttcatctaat cttaccagtc
     2401 tgtcttctgt aggatgtgtc actgttggat gttacaccag cctttccagg gttaaccact
     2461 gtggtggtgt gctgcttata gtttgctgtt gcattgtaat aaaaggtgtc tttccctgta
     2521 gtgacctgta aaaagtactc aagggcttta ttacagacat accctccctt tgaaaaggga
     2581 catgctaaaa gactcattac tactcagcct tcaatgtacc tgtgtgtcca tcttatattt
     2641 cttttttttt taattgtgaa ttagacttgt atatcccact gggagcactt tgtaggcatt
     2701 gcatgaacca tgggatgatg attctgtgga ggtattgcct tgtgaatttg ctgctatttt
     2761 agttttgtct ttgctgtaaa cttgtagcat taaacaatca ttgttgttaa taggtcttct
     2821 ttttgaaaca attatgtgaa atgtatagct gcttttgatg aaaagcagct atttgccttt
     2881 tttttttttt cctttgaact ttgaagctag tgcattggaa aaatgcaccc tttccctcct
     2941 ttggaatgct gtattaatgt agtataataa ttactggttt tgtaacttgt tctggtaatg
     3001 tccttcccgg actcttttta aatgtctccc cctaagtttt atacttgatt gtattattag
     3061 tctgttttta aatgttttgc ccggtttttc tcttcaatat ttgtgtatat aaaccgatct
     3121 tcgtgatact gtacatagct gtttgaaatg ccagaatgac ttctgacatt ccaagttttt
     3181 cacaaaatat attttatctg tgattagcca tttgactaat aatactggct aacagatgtt
     3241 gaaaaaaatt gtctgtttgt tttctcatta attttggtct aaaacatgtt tgcacttgtc
     3301 tttgacttgt gttttattaa cattgattgg catattaaaa gtcactctga gcttacctta
     3361 attgtctaaa tccttgtgat gcctgttttc taatatttta tctctattat tgctatacta
     3421 taaaatgtat agtgtgtata atgtactatt ataaaagcag agggcacatt ttgattgaat
     3481 atgaatatca cattgcatgt tatgcatgtg acttgctaga aatgtagcat gatgtaattt
     3541 aaaatatctt caaattatta agtgaatata atatgattca taactttc
//
