LOCUS       NM_016145                619 bp    mRNA    linear   PRI 27-NOV-2024
DEFINITION  Homo sapiens WD repeat domain 83 opposite strand (WDR83OS), mRNA.
ACCESSION   NM_016145
VERSION     NM_016145.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 619)
  AUTHORS   Sundaram,A., Yamsek,M., Zhong,F., Hooda,Y., Hegde,R.S. and
            Keenan,R.J.
  TITLE     Substrate-driven assembly of a translocon for multipass membrane
            proteins
  JOURNAL   Nature 611 (7934), 167-172 (2022)
   PUBMED   36261522
REFERENCE   2  (bases 1 to 619)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   3  (bases 1 to 619)
  AUTHORS   Chitwood,P.J. and Hegde,R.S.
  TITLE     An intramembrane chaperone complex facilitates membrane protein
            biogenesis
  JOURNAL   Nature 584 (7822), 630-634 (2020)
   PUBMED   32814900
REFERENCE   4  (bases 1 to 619)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   5  (bases 1 to 619)
  AUTHORS   Talbot,B.E., Vandorpe,D.H., Stotter,B.R., Alper,S.L. and
            Schlondorff,J.S.
  TITLE     Transmembrane insertases and N-glycosylation critically determine
            synthesis, trafficking, and activity of the nonselective cation
            channel TRPC6
  JOURNAL   J Biol Chem 294 (34), 12655-12669 (2019)
   PUBMED   31266804
REFERENCE   6  (bases 1 to 619)
  AUTHORS   Meacock,S.L., Lecomte,F.J., Crawshaw,S.G. and High,S.
  TITLE     Different transmembrane domains associate with distinct endoplasmic
            reticulum components during membrane integration of a polytopic
            protein
  JOURNAL   Mol Biol Cell 13 (12), 4114-4129 (2002)
   PUBMED   12475939
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AF078861.1.
            
            On Nov 22, 2018 this sequence version replaced NM_016145.3.
            
            ##Evidence-Data-START##
            Transcript exon combination :: ERR279836.6736.1, BC061912.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2142348, SAMEA2144333
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000596731.7/ ENSP00000468969.1
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-619               AF078861.1         222-840
FEATURES             Location/Qualifiers
     source          1..619
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="19"
                     /map="19p13.13"
     gene            1..619
                     /gene="WDR83OS"
                     /gene_synonym="ASTERIX; C19orf56; NEDFHCA; PAT-10; PAT10;
                     PTD008"
                     /note="WD repeat domain 83 opposite strand"
                     /db_xref="GeneID:51398"
                     /db_xref="HGNC:HGNC:30203"
                     /db_xref="MIM:618474"
     exon            1..62
                     /gene="WDR83OS"
                     /gene_synonym="ASTERIX; C19orf56; NEDFHCA; PAT-10; PAT10;
                     PTD008"
                     /inference="alignment:Splign:2.1.0"
     CDS             13..333
                     /gene="WDR83OS"
                     /gene_synonym="ASTERIX; C19orf56; NEDFHCA; PAT-10; PAT10;
                     PTD008"
                     /note="UPF0139 membrane protein C19orf56; WDR83 opposite
                     strand; protein Asterix; protein associated with the ER
                     translocon of 10kDa"
                     /codon_start=1
                     /product="PAT complex subunit Asterix"
                     /protein_id="NP_057229.1"
                     /db_xref="CCDS:CCDS12274.1"
                     /db_xref="GeneID:51398"
                     /db_xref="HGNC:HGNC:30203"
                     /db_xref="MIM:618474"
                     /translation="MSTNNMSDPRRPNKVLRYKPPPSECNPALDDPTPDYMNLLGMIF
                     SMCGLMLKLKWCAWVAVYCSFISFANSRSSEDTKQMMSSFMLSISAVVMSYLQNPQPM
                     TPPW"
     exon            63..168
                     /gene="WDR83OS"
                     /gene_synonym="ASTERIX; C19orf56; NEDFHCA; PAT-10; PAT10;
                     PTD008"
                     /inference="alignment:Splign:2.1.0"
     exon            169..266
                     /gene="WDR83OS"
                     /gene_synonym="ASTERIX; C19orf56; NEDFHCA; PAT-10; PAT10;
                     PTD008"
                     /inference="alignment:Splign:2.1.0"
     exon            267..619
                     /gene="WDR83OS"
                     /gene_synonym="ASTERIX; C19orf56; NEDFHCA; PAT-10; PAT10;
                     PTD008"
                     /inference="alignment:Splign:2.1.0"
     regulatory      601..606
                     /regulatory_class="polyA_signal_sequence"
                     /gene="WDR83OS"
                     /gene_synonym="ASTERIX; C19orf56; NEDFHCA; PAT-10; PAT10;
                     PTD008"
                     /note="hexamer: AATAAA"
     polyA_site      619
                     /gene="WDR83OS"
                     /gene_synonym="ASTERIX; C19orf56; NEDFHCA; PAT-10; PAT10;
                     PTD008"
                     /note="major polyA site"
ORIGIN      
        1 cttcgactcg ctatgtccac taacaatatg tcggacccac ggaggccgaa caaagtgctg
       61 aggtacaagc ccccgccgag cgaatgtaac ccggccttgg acgacccgac gccggactac
      121 atgaacctgc tgggcatgat cttcagcatg tgcggcctca tgcttaagct gaagtggtgt
      181 gcttgggtcg ctgtctactg ctccttcatc agctttgcca actctcggag ctcggaggac
      241 acgaagcaaa tgatgagtag cttcatgctg tccatctctg ccgtggtgat gtcctatctg
      301 cagaatcctc agcccatgac gcccccatgg tgataccagc ctagaagggt cacattttgg
      361 accctgtcta tccactaggc ctgggctttg gctgctaaac ctgctgcctt cagctgccat
      421 cctggacttc cctgaatgag gccgtctcgg tgcccccagc tggatagagg gaacctggcc
      481 ctttcctagg gaacacccta ggcttacccc tcctgcctcc cttcccctgc ctgctgctgg
      541 gggagatgct gtccatgttt ctaggggtat tcatttgctt tctcgttgaa acctgttgtt
      601 aataaagttt ttcactctg
//
