LOCUS       NM_001163809            6982 bp    mRNA    linear   PRI 01-JUN-2024
DEFINITION  Homo sapiens WD repeat domain 81 (WDR81), transcript variant 1,
            mRNA.
ACCESSION   NM_001163809
VERSION     NM_001163809.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6982)
  AUTHORS   Abdel-Hamid,M.S., Sabry,S., Abdel-Ghafar,S.F., El-Dessouky,S.H. and
            Abdel-Salam,G.M.H.
  TITLE     Fetal brain arrest broadens the spectrum of WDR81-related
            developmental brain malformations
  JOURNAL   Neurogenetics 22 (4), 287-295 (2021)
   PUBMED   34338917
  REMARK    GeneRIF: Fetal brain arrest broadens the spectrum of WDR81-related
            developmental brain malformations.
REFERENCE   2  (bases 1 to 6982)
  AUTHORS   Tadayoni Nia,A., Bazi,Z., Khosravi,A. and Oladnabi,M.
  TITLE     WDR81 Gene Silencing Can Reduce Exosome Levels in Human U87-MG
            Glioblastoma Cells
  JOURNAL   J Mol Neurosci 71 (8), 1696-1702 (2021)
   PUBMED   33954857
  REMARK    GeneRIF: WDR81 Gene Silencing Can Reduce Exosome Levels in Human
            U87-MG Glioblastoma Cells.
REFERENCE   3  (bases 1 to 6982)
  AUTHORS   Cavallin,M., Rujano,M.A., Bednarek,N., Medina-Cano,D., Bernabe
            Gelot,A., Drunat,S., Maillard,C., Garfa-Traore,M., Bole,C.,
            Nitschke,P., Beneteau,C., Besnard,T., Cogne,B., Eveillard,M.,
            Kuster,A., Poirier,K., Verloes,A., Martinovic,J., Bidat,L., Rio,M.,
            Lyonnet,S., Reilly,M.L., Boddaert,N., Jenneson-Liver,M., Motte,J.,
            Doco-Fenzy,M., Chelly,J., Attie-Bitach,T., Simons,M., Cantagrel,V.,
            Passemard,S., Baffet,A., Thomas,S. and Bahi-Buisson,N.
  TITLE     WDR81 mutations cause extreme microcephaly and impair mitotic
            progression in human fibroblasts and Drosophila neural stem cells
  JOURNAL   Brain 140 (10), 2597-2609 (2017)
   PUBMED   28969387
  REMARK    GeneRIF: The results of this study suggest that the WDR81 might
            have a role in mitosis that is conserved between Drosophila and
            humans.
REFERENCE   4  (bases 1 to 6982)
  AUTHORS   Shaheen,R., Sebai,M.A., Patel,N., Ewida,N., Kurdi,W., Altweijri,I.,
            Sogaty,S., Almardawi,E., Seidahmed,M.Z., Alnemri,A., Madirevula,S.,
            Ibrahim,N., Abdulwahab,F., Hashem,M., Al-Sheddi,T., Alomar,R.,
            Alobeid,E., Sallout,B., AlBaqawi,B., AlAali,W., Ajaji,N.,
            Lesmana,H., Hopkin,R.J., Dupuis,L., Mendoza-Londono,R., Al
            Rukban,H., Yoon,G., Faqeih,E. and Alkuraya,F.S.
  TITLE     The genetic landscape of familial congenital hydrocephalus
  JOURNAL   Ann Neurol 81 (6), 890-897 (2017)
   PUBMED   28556411
  REMARK    GeneRIF: Recessive mutations in WDR81, previously linked to
            cerebellar ataxia, mental retardation, and disequilibrium syndrome
            2, cause severe congenital hydrocephalus.
REFERENCE   5  (bases 1 to 6982)
  AUTHORS   Liu,X., Li,Y., Wang,X., Xing,R., Liu,K., Gan,Q., Tang,C., Gao,Z.,
            Jian,Y., Luo,S., Guo,W. and Yang,C.
  TITLE     The BEACH-containing protein WDR81 coordinates p62 and LC3C to
            promote aggrephagy
  JOURNAL   J Cell Biol 216 (5), 1301-1320 (2017)
   PUBMED   28404643
  REMARK    GeneRIF: The WDR81 interacts with LC3C through canonical
            LC3-interacting regions in the BEACH domain, promoting LC3C
            recruitment to ubiquitinated proteins.
REFERENCE   6  (bases 1 to 6982)
  AUTHORS   Sarac,O., Gulsuner,S., Yildiz-Tasci,Y., Ozcelik,T. and Kansu,T.
  TITLE     Neuro-ophthalmologic findings in humans with quadrupedal locomotion
  JOURNAL   Ophthalmic Genet 33 (4), 249-252 (2012)
   PUBMED   22686558
  REMARK    GeneRIF: The neuro-ophthalmic examination in CAMRQ2 revealed
            downbeat nystagmus in all patients, and temporal disc pallor and
            macular atrophy in two patients.
REFERENCE   7  (bases 1 to 6982)
  AUTHORS   Franceschini,N., van Rooij,F.J., Prins,B.P., Feitosa,M.F.,
            Karakas,M., Eckfeldt,J.H., Folsom,A.R., Kopp,J., Vaez,A.,
            Andrews,J.S., Baumert,J., Boraska,V., Broer,L., Hayward,C.,
            Ngwa,J.S., Okada,Y., Polasek,O., Westra,H.J., Wang,Y.A., Del
            Greco,M. F, Glazer,N.L., Kapur,K., Kema,I.P., Lopez,L.M.,
            Schillert,A., Smith,A.V., Winkler,C.A., Zgaga,L., Bandinelli,S.,
            Bergmann,S., Boban,M., Bochud,M., Chen,Y.D., Davies,G., Dehghan,A.,
            Ding,J., Doering,A., Durda,J.P., Ferrucci,L., Franco,O.H.,
            Franke,L., Gunjaca,G., Hofman,A., Hsu,F.C., Kolcic,I., Kraja,A.,
            Kubo,M., Lackner,K.J., Launer,L., Loehr,L.R., Li,G., Meisinger,C.,
            Nakamura,Y., Schwienbacher,C., Starr,J.M., Takahashi,A., Torlak,V.,
            Uitterlinden,A.G., Vitart,V., Waldenberger,M., Wild,P.S., Kirin,M.,
            Zeller,T., Zemunik,T., Zhang,Q., Ziegler,A., Blankenberg,S.,
            Boerwinkle,E., Borecki,I.B., Campbell,H., Deary,I.J.,
            Frayling,T.M., Gieger,C., Harris,T.B., Hicks,A.A., Koenig,W., O'
            Donnell,C.J., Fox,C.S., Pramstaller,P.P., Psaty,B.M., Reiner,A.P.,
            Rotter,J.I., Rudan,I., Snieder,H., Tanaka,T., van Duijn,C.M.,
            Vollenweider,P., Waeber,G., Wilson,J.F., Witteman,J.C.,
            Wolffenbuttel,B.H., Wright,A.F., Wu,Q., Liu,Y., Jenny,N.S.,
            North,K.E., Felix,J.F., Alizadeh,B.Z., Cupples,L.A., Perry,J.R. and
            Morris,A.P.
  CONSRTM   LifeLines Cohort Study
  TITLE     Discovery and fine mapping of serum protein loci through
            transethnic meta-analysis
  JOURNAL   Am J Hum Genet 91 (4), 744-753 (2012)
   PUBMED   23022100
REFERENCE   8  (bases 1 to 6982)
  AUTHORS   Gulsuner,S., Tekinay,A.B., Doerschner,K., Boyaci,H., Bilguvar,K.,
            Unal,H., Ors,A., Onat,O.E., Atalar,E., Basak,A.N., Topaloglu,H.,
            Kansu,T., Tan,M., Tan,U., Gunel,M. and Ozcelik,T.
  TITLE     Homozygosity mapping and targeted genomic sequencing reveal the
            gene responsible for cerebellar hypoplasia and quadrupedal
            locomotion in a consanguineous kindred
  JOURNAL   Genome Res 21 (12), 1995-2003 (2011)
   PUBMED   21885617
REFERENCE   9  (bases 1 to 6982)
  AUTHORS   O'Seaghdha,C.M., Yang,Q., Glazer,N.L., Leak,T.S., Dehghan,A.,
            Smith,A.V., Kao,W.H., Lohman,K., Hwang,S.J., Johnson,A.D.,
            Hofman,A., Uitterlinden,A.G., Chen,Y.D., Brown,E.M.,
            Siscovick,D.S., Harris,T.B., Psaty,B.M., Coresh,J., Gudnason,V.,
            Witteman,J.C., Liu,Y.M., Kestenbaum,B.R., Fox,C.S. and Kottgen,A.
  CONSRTM   GEFOS Consortium
  TITLE     Common variants in the calcium-sensing receptor gene are associated
            with total serum calcium levels
  JOURNAL   Hum Mol Genet 19 (21), 4296-4303 (2010)
   PUBMED   20705733
REFERENCE   10 (bases 1 to 6982)
  AUTHORS   Hendrickx,A., Beullens,M., Ceulemans,H., Den Abt,T., Van Eynde,A.,
            Nicolaescu,E., Lesage,B. and Bollen,M.
  TITLE     Docking motif-guided mapping of the interactome of protein
            phosphatase-1
  JOURNAL   Chem Biol 16 (4), 365-371 (2009)
   PUBMED   19389623
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC130343.7, AK122972.1,
            BC114519.1 and AK123896.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Dec 23, 2019 this sequence version replaced NM_001163809.1.
            
            Summary: This gene encodes a multi-domain transmembrane protein
            which is predominantly expressed in the brain and is thought to
            play a role in endolysosomal trafficking. Mutations in this gene
            are associated with an autosomal recessive form of a syndrome
            exhibiting cerebellar ataxia, cognitive disability, and
            disequilibrium (CAMRQ2). Alternatively spliced transcript variants
            encoding different isoforms have been found for this gene.
            [provided by RefSeq, Jul 2017].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            Sequence Note: The RefSeq transcript and protein were derived from
            transcript and genomic sequence data to make the sequence
            consistent with the reference genome assembly. The genomic
            coordinates used for the transcript record were based on
            alignments. The 5' coding exon is inferred based on available
            transcript data and further supported by similar exon-intron
            structure of the related mouse gene, GeneID 192652.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC092513.1, SRR14038197.1749132.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000409644.6/ ENSP00000386609.1
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2865              AC130343.7         174218-177082
            2866-4552           AK122972.1         1-1687
            4553-5226           BC114519.1         636-1309
            5227-6823           AK123896.1         1542-3138
            6824-6982           AK122972.1         3959-4117
FEATURES             Location/Qualifiers
     source          1..6982
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="17"
                     /map="17p13.3"
     gene            1..6982
                     /gene="WDR81"
                     /gene_synonym="CAMRQ2; CHMRQ; HYC3; PPP1R166; SORF-2"
                     /note="WD repeat domain 81"
                     /db_xref="GeneID:124997"
                     /db_xref="HGNC:HGNC:26600"
                     /db_xref="MIM:614218"
     exon            1..3923
                     /gene="WDR81"
                     /gene_synonym="CAMRQ2; CHMRQ; HYC3; PPP1R166; SORF-2"
                     /inference="alignment:Splign:2.1.0"
     CDS             257..6082
                     /gene="WDR81"
                     /gene_synonym="CAMRQ2; CHMRQ; HYC3; PPP1R166; SORF-2"
                     /note="isoform 1 is encoded by transcript variant 1; WD
                     repeat-containing protein 81; protein phosphatase 1,
                     regulatory subunit 166"
                     /codon_start=1
                     /product="WD repeat-containing protein 81 isoform 1"
                     /protein_id="NP_001157281.1"
                     /db_xref="CCDS:CCDS54062.1"
                     /db_xref="GeneID:124997"
                     /db_xref="HGNC:HGNC:26600"
                     /db_xref="MIM:614218"
                     /translation="MAQGSGGREGALRTPAGGWHSPPSPDMQELLRSVERDLSIDPRQ
                     LAPAPGGTHVVALVPARWLASLRDRRLPLGPCPRAEGLGEAEVRTLLQRSVQRLPAGW
                     TRVEVHGLRKRRLSYPLGGGLPFEDGSCGPETLTRFMQEVAAQNYRNLWRHAYHTYGQ
                     PYSHSPAPSAVPALDSVRQALQRVYGCSFLPVGETTQCPSYAREGPCPPRGSPACPSL
                     LRAEALLESPEMLYVVHPYVQFSLHDVVTFSPAKLTNSQAKVLFILFRVLRAMDACHR
                     QGLACGALSLYHIAVDEKLCSELRLDLSAYERPEEDENEEAPVARDEAGIVSQEEQGG
                     QPGQPTGQEELRSLVLDWVHGRISNFHYLMQLNRLAGRRQGDPNYHPVLPWVVDFTTP
                     HGRFRDLRKSKFRLNKGDKQLDFTYEMTRQAFVAGGAGGGEPPHVPHHISDVLSDITY
                     YVYKARRTPRSVLCGHVRAQWEPHEYPASMERMQNWTPDECIPEFYTDPSIFRSIHPD
                     MPDLDVPAWCSSSQEFVAAHRALLESREVSRDLHHWIDLTFGYKLQGKEAVKEKNVCL
                     HLVDAHTHLASYGVVQLFDQPHPQRLAGAPALAPEPPLIPKLLVQTIQETTGREDFTE
                     NPGQLPNGVGRPVLEATPCEASWTRDRPVAGEDDLEQATEALDSISLAGKAGDQLGSS
                     SQASPGLLSFSVASASRPGRRNKAAGADPGEGEEGRILLPEGFNPMQALEELEKTGNF
                     LAKGLGGLLEVPEQPRVQPAVPLQCLLHRDMQALGVLLAEMVFATRVRTLQPDAPLWV
                     RFQAVRGLCTRHPKEVPVSLQPVLDTLLQMSGPEVPMGAERGKLDQLFEYRPVSQGLP
                     PPCPSQLLSPFSSVVPFPPYFPALHRFILLYQARRVEDEAQGRELVFALWQQLGAVLK
                     DITPEGLEILLPFVLSLMSEEHTAVYTAWYLFEPVAKALGPKNANKYLLKPLIGAYES
                     PCQLHGRFYLYTDCFVAQLMVRLGLQAFLTHLLPHVLQVLAGAEASQEESKDLAGAAE
                     EEESGLPGAGPGSCAFGEEIPMDGEPPASSGLGLPDYTSGVSFHDQADLPETEDFQAG
                     LYVTESPQPQEAEAVSLGRLSDKSSTSETSLGEERAPDEGGAPVDKSSLRSGDSSQDL
                     KQSEGSEEEEEEEDSCVVLEEEEGEQEEVTGASELTLSDTVLSMETVVAGGSGGDGEE
                     EEEALPEQSEGKEQKILLDTACKMVRWLSAKLGPTVASRHVARNLLRLLTSCYVGPTR
                     QQFTVSSGESPPLSAGNIYQKRPVLGDIVSGPVLSCLLHIARLYGEPVLTYQYLPYIS
                     YLVAPGSASGPSRLNSRKEAGLLAAVTLTQKIIVYLSDTTLMDILPRISHEVLLPVLS
                     FLTSLVTGFPSGAQARTILCVKTISLIALICLRIGQEMVQQHLSEPVATFFQVFSQLH
                     ELRQQDLKLDPAGRGEGQLPQVVFSDGQQRPVDPALLDELQKVFTLEMAYTIYVPFSC
                     LLGDIIRKIIPNHELVGELAALYLESISPSSRNPASVEPTMPGTGPEWDPHGGGCPQD
                     DGHSGTFGSVLVGNRIQIPNDSRPENPGPLGPISGVGGGGLGSGSDDNALKQELPRSV
                     HGLSGNWLAYWQYEIGVSQQDAHFHFHQIRLQSFPGHSGAVKCVAPLSSEDFFLSGSK
                     DRTVRLWPLYNYGDGTSETAPRLVYTQHRKSVFFVGQLEAPQHVVSCDGAVHVWDPFT
                     GKTLRTVEPLDSRVPLTAVAVMPAPHTSITMASSDSTLRFVDCRKPGLQHEFRLGGGL
                     NPGLVRALAISPSGRSVVAGFSSGFMVLLDTRTGLVLRGWPAHEGDILQIKAVEGSVL
                     VSSSSDHSLTVWKELEQKPTHHYKSASDPIHTFDLYGSEVVTGTVSNKIGVCSLLEPP
                     SQATTKLSSENFRGTLTSLALLPTKRHLLLGSDNGVIRLLA"
     exon            3924..4031
                     /gene="WDR81"
                     /gene_synonym="CAMRQ2; CHMRQ; HYC3; PPP1R166; SORF-2"
                     /inference="alignment:Splign:2.1.0"
     exon            4032..4222
                     /gene="WDR81"
                     /gene_synonym="CAMRQ2; CHMRQ; HYC3; PPP1R166; SORF-2"
                     /inference="alignment:Splign:2.1.0"
     exon            4223..4413
                     /gene="WDR81"
                     /gene_synonym="CAMRQ2; CHMRQ; HYC3; PPP1R166; SORF-2"
                     /inference="alignment:Splign:2.1.0"
     exon            4414..4579
                     /gene="WDR81"
                     /gene_synonym="CAMRQ2; CHMRQ; HYC3; PPP1R166; SORF-2"
                     /inference="alignment:Splign:2.1.0"
     exon            4580..4745
                     /gene="WDR81"
                     /gene_synonym="CAMRQ2; CHMRQ; HYC3; PPP1R166; SORF-2"
                     /inference="alignment:Splign:2.1.0"
     exon            4746..5435
                     /gene="WDR81"
                     /gene_synonym="CAMRQ2; CHMRQ; HYC3; PPP1R166; SORF-2"
                     /inference="alignment:Splign:2.1.0"
     exon            5436..5581
                     /gene="WDR81"
                     /gene_synonym="CAMRQ2; CHMRQ; HYC3; PPP1R166; SORF-2"
                     /inference="alignment:Splign:2.1.0"
     exon            5582..5761
                     /gene="WDR81"
                     /gene_synonym="CAMRQ2; CHMRQ; HYC3; PPP1R166; SORF-2"
                     /inference="alignment:Splign:2.1.0"
     exon            5762..6982
                     /gene="WDR81"
                     /gene_synonym="CAMRQ2; CHMRQ; HYC3; PPP1R166; SORF-2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6961..6966
                     /regulatory_class="polyA_signal_sequence"
                     /gene="WDR81"
                     /gene_synonym="CAMRQ2; CHMRQ; HYC3; PPP1R166; SORF-2"
                     /note="hexamer: AATAAA"
     polyA_site      6982
                     /gene="WDR81"
                     /gene_synonym="CAMRQ2; CHMRQ; HYC3; PPP1R166; SORF-2"
                     /note="major polyA site"
ORIGIN      
        1 agctgacccg tcacggtgga gcccggtgct cgcgcccggc agcctctgcc ccgccgcgcc
       61 cggagcgcag gacccgcgga ggggtaagcg cgccccccgt ccgcctcttc gccgccgccg
      121 gcttcctgcg gccgcctccg ccccagcccc tgtcccgcgc ccatcccagc cccgccggcc
      181 tggcaccccg gaagccgtcg ccagcagggc cgtggctggg ctcagccccg cgctgccccc
      241 gggcggcctg gaggagatgg cccagggcag cggggggcgg gaaggcgctc tcagaacccc
      301 ggccgggggc tggcattccc cgccaagccc agacatgcag gagctgctcc ggagcgtgga
      361 gagggacctg agcatcgatc ccaggcagct ggctccggcc ccggggggca cccacgtggt
      421 ggccctagtg cctgcgcgct ggctggccag cctccgcgat cgccggctgc ccctgggacc
      481 ctgtccccgc gcagagggcc tgggagaagc ggaagtcagg actctcctgc agcgctctgt
      541 gcaaaggctg cctgccggct ggacgcgcgt ggaggtgcat gggctgcgga agcggagact
      601 gtcctaccct ctgggcgggg gcctgccctt tgaggacggg tcctgcggcc ctgagaccct
      661 cactcgcttc atgcaggagg ttgccgccca gaattatcgc aacctgtggc gccatgcata
      721 ccacacttac ggccagccgt acagtcacag ccctgccccc tcagctgtcc ctgccttgga
      781 ctcagtacgg caggctctgc agagggtcta tggttgctcc ttcctgccag tgggtgaaac
      841 tacccaatgc ccttcatatg ccagagaagg cccctgcccc cctcggggca gccctgcttg
      901 ccctagtctt ttacgggctg aggccttgct ggagtcgccg gagatgctgt atgtggtaca
      961 cccttacgta cagttctccc tacatgacgt ggtcaccttc agccctgcca agctgaccaa
     1021 cagccaggcc aaggtgctgt tcattctctt ccgcgtgctg agggctatgg acgcctgtca
     1081 ccgccagggg ctggcgtgtg gggccctgtc tttgtatcac atcgcagtgg atgagaagct
     1141 ttgcagcgag ctgcgactgg acctgagtgc ttatgagagg cccgaggagg acgagaatga
     1201 ggaggcccct gtggcaaggg atgaggcggg cattgtgtct caagaggagc agggagggca
     1261 acctgggcaa cccactggcc aggaggaact tcggagcctc gtgctagatt gggtccacgg
     1321 ccgcatcagc aacttccact acctcatgca gctgaatcgg ttggcaggtc ggcggcaggg
     1381 ggaccccaac taccaccccg tgctgccctg ggtggtggac ttcactacgc cccatgggcg
     1441 cttccgagac ctgcgcaagt ccaagttccg cctcaacaag ggggataagc aactggactt
     1501 cacgtatgag atgacacggc aggcattcgt agcaggcggg gcgggcggcg gggaaccccc
     1561 tcatgttccc caccacatct cagacgtgct ctccgacatc acgtactatg tgtacaaggc
     1621 tcggcgcacg cctcggtcgg tgctctgcgg acacgtccgc gcgcagtggg agccccatga
     1681 gtatccggcc agcatggagc ggatgcagaa ctggaccccg gatgagtgca ttccggagtt
     1741 ctacaccgat ccctctatct tccgctccat ccaccccgac atgcctgacc tggatgtgcc
     1801 agcctggtgc agctccagcc aggagttcgt agctgcccac cgagccctgc tggagagccg
     1861 cgaggtatcc cgggacctgc accattggat cgacctcacg tttggctata aactccaggg
     1921 taaggaggct gtcaaggaaa agaatgtgtg tctgcacctg gtggacgccc acactcacct
     1981 ggccagctac ggggtggtgc agctcttcga tcagccacac ccccagcgcc tggctggggc
     2041 tcctgccctt gcccccgagc ctcccctcat ccccaagctg ttggtccaga ccatccagga
     2101 gaccacaggc cgggaggact tcacggaaaa cccgggacag cttccaaatg gagtgggccg
     2161 gccagtttta gaggccactc cctgtgaggc tagctggacc agagacaggc cggtggcagg
     2221 agaagacgac ttggaacagg ccacagaagc tctggattcc atttcccttg ctgggaaagc
     2281 aggtgaccag ctgggctcct ccagtcaagc gtcccctgga cttctctctt tctcagtggc
     2341 ctcagcctcc cgtccaggcc gcaggaataa agctgctggg gcagaccctg gggagggtga
     2401 ggaggggagg attcttcttc ccgagggctt caatcccatg caggccctgg aggagctgga
     2461 gaaaacgggc aacttcttgg ccaaaggcct agggggcctg ttggaggtgc ctgagcagcc
     2521 ccgggtccag ccggctgtgc cactgcagtg cctactccac agggacatgc aggcgctggg
     2581 tgtcctattg gcagagatgg tgtttgccac cagggtgcgg acgctgcagc ccgatgcacc
     2641 tttgtgggta cgcttccagg ctgtccgagg gctctgcacg cgccacccca aggaggtccc
     2701 tgtgtctttg cagcccgtgc tggacacact cctgcagatg agtggccccg aagtccccat
     2761 gggagcagag aggggcaagc tggaccaact gtttgagtac aggcctgtct cccagggcct
     2821 gcccccaccc tgcccaagcc agcttctcag ccccttcagc tccgtggttc ccttcccacc
     2881 ctacttcccg gcactgcaca gattcatcct cctgtaccag gcaaggcgtg tggaggacga
     2941 ggcccagggg cgcgagctgg tgtttgctct gtggcagcag ctgggcgcgg tgctgaagga
     3001 catcacccct gagggcctgg agatcctgct gcccttcgtg ctctcactca tgtccgagga
     3061 gcacacagct gtgtacacgg cctggtatct gtttgagcct gttgccaagg cactgggccc
     3121 caaaaatgcc aataagtacc tcctgaagcc gctcattggt gcctacgaga gcccctgcca
     3181 gctacacggc cgcttctacc tgtacacgga ctgctttgtg gcccagctga tggtgcggct
     3241 gggcctgcag gcatttctca ctcacctgct gccccatgtc ctgcaggtgc tggcgggcgc
     3301 agaggcctcc caggaggaga gcaaggacct ggcaggggct gctgaggagg aggagagcgg
     3361 gctgcccggg gccgggcctg gctcctgtgc ttttggggag gagattccca tggatgggga
     3421 gcctcctgcc tcctcgggcc tggggctccc agactacacg tctggcgtca gcttccacga
     3481 ccaggctgac ctccctgaga cagaggactt ccaagccggg ctctatgtga ctgagtctcc
     3541 ccagccccag gaggctgagg ctgtgagcct gggccggctg agtgacaaga gcagcaccag
     3601 cgagacctcc ctgggtgagg agcgggctcc agacgagggg ggtgcccccg tggacaagag
     3661 cagccttcga tcaggtgaca gcagccagga cttgaagcaa agcgagggct ccgaggagga
     3721 agaggaggag gaggacagct gcgtggtgct agaggaggag gagggggagc aggaggaggt
     3781 caccggggca tctgagctca ctctgtctga cacggtgctg tccatggaga cggttgtggc
     3841 cggcggcagt gggggagatg gagaagaaga ggaggaggca ctgcctgagc agtcagaagg
     3901 caaagaacag aagatcctcc ttgatacagc ctgcaagatg gtccgctggc tgtctgccaa
     3961 gctcggcccc acagtggcct ctcgccacgt ggcccggaac ctgctccgcc tgctgacgtc
     4021 ttgttatgtt ggacccactc ggcagcagtt cacagtgagc agtggcgaga gcccaccgct
     4081 gagcgccggc aacatctacc agaagaggcc ggtcctgggc gacatcgtgt cagggcctgt
     4141 gctcagctgc ctcctccaca tcgcccgcct gtatggggag cctgtcctca cctaccagta
     4201 cctgccctac atcagctacc tggtggcccc agggagtgcc tcaggcccca gccgactgaa
     4261 cagccgtaag gaggcggggc tgctggccgc ggtgacgctg actcagaaga tcatcgtgta
     4321 cctctcagac accacactca tggacatcct gccccggatc agccatgagg tcctgctgcc
     4381 cgtgctcagc ttcctcacct ccctcgtcac ggggttccca agtggggccc aggctcggac
     4441 catcctgtgt gtgaaaacca tcagcctcat cgccctcatc tgcctgcgca ttggacagga
     4501 gatggtccag cagcacctga gcgagcccgt ggccaccttt ttccaggtct tctctcagct
     4561 gcatgagctt cggcaacagg atctgaagct ggaccctgcg ggccgtggtg agggccagct
     4621 gccacaggtg gtcttctctg atgggcagca gcggcccgtg gaccccgccc tgctggacga
     4681 gctgcagaag gtgttcaccc tggagatggc atacacaatc tacgtgccct tctcctgcct
     4741 gttgggtgac atcatccgga aaatcatccc caaccacgag ctggttgggg agctggcggc
     4801 gctgtacttg gagagcatca gccccagcag tcgcaaccct gccagcgtgg agcccaccat
     4861 gcccggcacc gggcccgagt gggaccccca tggtgggggc tgccctcagg atgacggcca
     4921 ctcagggacc tttgggagcg tcctggtggg gaaccgcatt cagatcccca atgactctcg
     4981 gcctgagaac cccggaccac tgggccccat ctcgggggtg ggtggcgggg gcctgggcag
     5041 cgggagcgac gacaacgccc tgaagcagga gctgccgcgg agcgtgcacg ggctgagcgg
     5101 aaactggctg gcgtactggc agtacgagat cggcgtgagc cagcaggatg cccactttca
     5161 cttccaccag atccgcctgc agagcttccc gggccactcg ggggccgtca agtgcgtggc
     5221 acccctaagc agcgaggact tcttcctgag cggcagcaag gatcgtaccg tgcgcctctg
     5281 gccgctgtac aactacggcg acgggaccag cgagacggcc ccacgcctcg tctacaccca
     5341 gcaccgcaag agcgtcttct tcgtgggcca gcttgaggcc ccgcagcacg tggtgagctg
     5401 tgacggggct gtgcacgtct gggacccctt cacagggaag acccttcgca cagtggagcc
     5461 gctggacagc cgggtgcccc tgactgcggt ggctgtcatg cccgcccccc acaccagcat
     5521 caccatggcc agctctgact ctaccctgcg ctttgtggac tgcaggaagc ctggtctgca
     5581 gcacgagttc cgactgggcg gtgggctgaa ccctgggctt gtccgtgccc tggccatcag
     5641 ccccagtggc cgtagtgtcg tggccggctt ctcctcaggc ttcatggtgc tcctggacac
     5701 ccgcacaggc ctggttctgc gaggctggcc agcccacgag ggggacattc tgcagatcaa
     5761 ggcggtggag ggcagcgtcc tggtcagctc ctcctctgac cattccttga ccgtctggaa
     5821 ggagctggag cagaagccca cccatcacta caagtcagca tccgacccca tccacacctt
     5881 tgacctgtac ggcagcgagg tggtcactgg caccgtgtcc aacaagattg gcgtctgctc
     5941 cctgcttgag ccaccctcgc aggccaccac gaagctcagc tctgagaact tccgcggcac
     6001 gctcaccagc ctggccttgc tgcccactaa acgccacctc ctgctgggct cagacaacgg
     6061 ggttatccgc ctcctggcat agactgaggc aggagctggc cgggcaaggg tgggaagaca
     6121 tctgcgggcg cgtgtccact caccctgttc cctgagcagc agctccctcc agggaggccc
     6181 tgggtcccac gccctgggtg cccacatggc ctgccaacta gggcctgcaa atggagtggg
     6241 ggagtcctgg cccctgaatc accagagcca ccaagcctgc cagaggggtc tcattcatgg
     6301 cttggggaca cagggctcct agcaagcagg aagttaagag caggaggaag cgttgctacc
     6361 ttcacttctc cccagctctg ccctctgggt ccacatgagg acagggaagc tcgggaaggg
     6421 gaagggagac tggccctgcc cagccggtct ctagcccctc agcccccgct gggcactctc
     6481 tgtcccatcc ctctaggaca gggaagctgg cctggtccag ggcactgatg gtgcttggat
     6541 tccagcctaa ggaaggctgg ccgtggtcca ggagttaagg gcttgggtct ggggtttaag
     6601 tggccaccca tccaggccct ggccagtgtg ggaccgggac gggaaggaag aaggaggcta
     6661 ggagcagggg gaaaaggtgc acttggccag tggcgcctgc caggagtgag tccatgcgtt
     6721 gtctgcccac ccctaccaca gtgtttgtgc cttcagctga gggggcagcc tctgggccct
     6781 gaacccctgc tggggctcca cgaccctgag agaaggggtg agaagaatca tctctgcacc
     6841 tcgggtctct gccagaggaa gacttaagca tccctgcgac ctcacattct agacagagat
     6901 gaggtccagg ggttggcccc tgctgccttc tcacaatttg caatagatgt aaataggacc
     6961 aataaatcct ttggaagagc ca
//
