LOCUS       NM_001369450            1219 bp    mRNA    linear   PRI 05-APR-2024
DEFINITION  Homo sapiens WD repeat domain 74 (WDR74), transcript variant 7,
            mRNA.
ACCESSION   NM_001369450 NM_001369452
VERSION     NM_001369450.1
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1219)
  AUTHORS   Gao,F., Zhou,H., Huang,X., Xie,H., Zhou,L., Chen,J. and Zheng,S.
  TITLE     WDR74 serves as a novel therapeutic target by its oncogenic role in
            hepatocellular carcinoma
  JOURNAL   Pathol Res Pract 248, 154622 (2023)
   PUBMED   37331183
  REMARK    GeneRIF: WDR74 serves as a novel therapeutic target by its
            oncogenic role in hepatocellular carcinoma.
REFERENCE   2  (bases 1 to 1219)
  AUTHORS   Wu,F., Wu,H., Hu,W., Zhang,Z. and Zhang,X.
  TITLE     WDR74 rs11231247 contributes to the susceptibility and prognosis of
            non-small cell lung cancer
  JOURNAL   Pathol Res Pract 242, 154318 (2023)
   PUBMED   36701849
  REMARK    GeneRIF: WDR74 rs11231247 contributes to the susceptibility and
            prognosis of non-small cell lung cancer.
REFERENCE   3  (bases 1 to 1219)
  AUTHORS   Li,Y., Zhou,Y., Li,B., Chen,F., Shen,W., Lu,Y., Zhong,C., Zhang,C.,
            Xie,H., Katanaev,V.L. and Jia,L.
  TITLE     WDR74 modulates melanoma tumorigenesis and metastasis through the
            RPL5-MDM2-p53 pathway
  JOURNAL   Oncogene 39 (13), 2741-2755 (2020)
   PUBMED   32005977
  REMARK    GeneRIF: WDR74 modulates melanoma tumorigenesis and metastasis
            through the RPL5-MDM2-p53 pathway.
REFERENCE   4  (bases 1 to 1219)
  AUTHORS   Li,Y., Chen,F., Shen,W., Li,B., Xiang,R., Qu,L., Zhang,C., Li,G.,
            Xie,H., Katanaev,V.L. and Jia,L.
  TITLE     WDR74 induces nuclear beta-catenin accumulation and activates
            Wnt-responsive genes to promote lung cancer growth and metastasis
  JOURNAL   Cancer Lett 471, 103-115 (2020)
   PUBMED   31838084
  REMARK    GeneRIF: WDR74 induces nuclear beta-catenin accumulation and
            activates Wnt-responsive genes to promote lung cancer growth and
            metastasis.
REFERENCE   5  (bases 1 to 1219)
  AUTHORS   Liu,J., Zhao,M., Yuan,B., Gu,S., Zheng,M., Zou,J., Jin,J., Liu,T.
            and Feng,X.H.
  TITLE     WDR74 functions as a novel coactivator in TGF-beta signaling
  JOURNAL   J Genet Genomics 45 (12), 639-650 (2018)
   PUBMED   30594465
  REMARK    GeneRIF: Through direct interactions with Smad proteins, WDR74
            enhances TGF-beta-mediated phosphorylation and nuclear accumulation
            of Smad2 and Smad3.
REFERENCE   6  (bases 1 to 1219)
  AUTHORS   Lo,Y.H., Romes,E.M., Pillon,M.C., Sobhany,M. and Stanley,R.E.
  TITLE     Structural Analysis Reveals Features of Ribosome Assembly Factor
            Nsa1/WDR74 Important for Localization and Interaction with
            Rix7/NVL2
  JOURNAL   Structure 25 (5), 762-772 (2017)
   PUBMED   28416111
REFERENCE   7  (bases 1 to 1219)
  AUTHORS   Hiraishi,N., Ishida,Y. and Nagahama,M.
  TITLE     AAA-ATPase NVL2 acts on MTR4-exosome complex to dissociate the
            nucleolar protein WDR74
  JOURNAL   Biochem Biophys Res Commun 467 (3), 534-540 (2015)
   PUBMED   26456651
  REMARK    GeneRIF: results suggest that WDR74 is a novel regulatory protein
            of the MTR4-exsosome complex whose interaction is regulated by NVL2
            and is involved in ribosome biogenesis
REFERENCE   8  (bases 1 to 1219)
  AUTHORS   Eilbracht,J., Reichenzeller,M., Hergt,M., Schnolzer,M., Heid,H.,
            Stohr,M., Franke,W.W. and Schmidt-Zachmann,M.S.
  TITLE     NO66, a highly conserved dual location protein in the nucleolus and
            in a special type of synchronously replicating chromatin
  JOURNAL   Mol Biol Cell 15 (4), 1816-1832 (2004)
   PUBMED   14742713
REFERENCE   9  (bases 1 to 1219)
  AUTHORS   Scherl,A., Coute,Y., Deon,C., Calle,A., Kindbeiter,K.,
            Sanchez,J.C., Greco,A., Hochstrasser,D. and Diaz,J.J.
  TITLE     Functional proteomic analysis of human nucleolus
  JOURNAL   Mol Biol Cell 13 (11), 4100-4109 (2002)
   PUBMED   12429849
REFERENCE   10 (bases 1 to 1219)
  AUTHORS   Andersen,J.S., Lyon,C.E., Fox,A.H., Leung,A.K., Lam,Y.W., Steen,H.,
            Mann,M. and Lamond,A.I.
  TITLE     Directed proteomic analysis of the human nucleolus
  JOURNAL   Curr Biol 12 (1), 1-11 (2002)
   PUBMED   11790298
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AP001160.4.
            
            On May 20, 2019 this sequence version replaced NM_001369452.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR5189658.131235.1,
                                           SRR7410570.472165.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000278856.9/ ENSP00000278856.4
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-84                AP001160.4         116808-116891       c
            85-191              AP001160.4         116623-116729       c
            192-313             AP001160.4         116415-116536       c
            314-389             AP001160.4         113262-113337       c
            390-536             AP001160.4         112996-113142       c
            537-638             AP001160.4         112732-112833       c
            639-739             AP001160.4         111728-111828       c
            740-795             AP001160.4         111577-111632       c
            796-941             AP001160.4         111093-111238       c
            942-998             AP001160.4         110919-110975       c
            999-1219            AP001160.4         110212-110432       c
FEATURES             Location/Qualifiers
     source          1..1219
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="11"
                     /map="11q12.3"
     gene            1..1219
                     /gene="WDR74"
                     /gene_synonym="Nsa1"
                     /note="WD repeat domain 74"
                     /db_xref="GeneID:54663"
                     /db_xref="HGNC:HGNC:25529"
                     /db_xref="MIM:617947"
     exon            1..84
                     /gene="WDR74"
                     /gene_synonym="Nsa1"
                     /inference="alignment:Splign:2.1.0"
     CDS             21..1178
                     /gene="WDR74"
                     /gene_synonym="Nsa1"
                     /note="isoform 1 is encoded by transcript variant 7; WD
                     repeat-containing protein 74; NOP seven-associated protein
                     1"
                     /codon_start=1
                     /product="WD repeat-containing protein 74 isoform 1"
                     /protein_id="NP_001356379.1"
                     /db_xref="CCDS:CCDS44630.1"
                     /db_xref="GeneID:54663"
                     /db_xref="HGNC:HGNC:25529"
                     /db_xref="MIM:617947"
                     /translation="MAAAAARWNHVWVGTETGILKGVNLQRKQAANFTAGGQPRREEA
                     VSALCWGTGGETQMLVGCADRTVKHFSTEDGIFQGQRHCPGGEGMFRGLAQADGTLIT
                     CVDSGILRVWHDKDKDTSSDPLLELRVGPGVCRMRQDPAHPHVVATGGKENALKIWDL
                     QGSEEPVFRAKNVRNDWLDLRVPIWDQDIQFLPGSQKLVTCTGYHQVRVYDPASPQRR
                     PVLETTYGEYPLTAMTLTPGGNSVIVGNTHGQLAEIDLRQGRLLGCLKGLAGSVRGLQ
                     CHPSKPLLASCGLDRVLRIHRIQNPRGLEHKVYLKSQLNCLLLSGRDNWEDEPQEPQE
                     PNKVPLEDTETDELWASLEAAAKRKLSGLEQPQGALQTRRRKKKRPGSTSP"
     exon            85..191
                     /gene="WDR74"
                     /gene_synonym="Nsa1"
                     /inference="alignment:Splign:2.1.0"
     exon            192..313
                     /gene="WDR74"
                     /gene_synonym="Nsa1"
                     /inference="alignment:Splign:2.1.0"
     exon            314..389
                     /gene="WDR74"
                     /gene_synonym="Nsa1"
                     /inference="alignment:Splign:2.1.0"
     exon            390..536
                     /gene="WDR74"
                     /gene_synonym="Nsa1"
                     /inference="alignment:Splign:2.1.0"
     exon            537..638
                     /gene="WDR74"
                     /gene_synonym="Nsa1"
                     /inference="alignment:Splign:2.1.0"
     exon            639..739
                     /gene="WDR74"
                     /gene_synonym="Nsa1"
                     /inference="alignment:Splign:2.1.0"
     exon            740..795
                     /gene="WDR74"
                     /gene_synonym="Nsa1"
                     /inference="alignment:Splign:2.1.0"
     exon            796..941
                     /gene="WDR74"
                     /gene_synonym="Nsa1"
                     /inference="alignment:Splign:2.1.0"
     exon            942..998
                     /gene="WDR74"
                     /gene_synonym="Nsa1"
                     /inference="alignment:Splign:2.1.0"
     exon            999..1219
                     /gene="WDR74"
                     /gene_synonym="Nsa1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1200..1205
                     /regulatory_class="polyA_signal_sequence"
                     /gene="WDR74"
                     /gene_synonym="Nsa1"
                     /note="hexamer: AATAAA"
     polyA_site      1219
                     /gene="WDR74"
                     /gene_synonym="Nsa1"
                     /note="major polyA site"
ORIGIN      
        1 gtctgcctcc aggctttgtc atggcggctg ctgctgcacg ctggaaccat gtgtgggtcg
       61 gcaccgagac tgggatcttg aaaggggtaa atcttcagcg aaaacaggcg gcgaacttca
      121 cggccggagg acagccgcgg cgcgaggagg cagtgagcgc cctgtgttgg ggcaccggcg
      181 gcgagaccca gatgctggtg ggctgcgcgg acaggacggt gaagcacttc agcaccgagg
      241 atggcatatt ccagggtcag agacactgcc cgggcgggga gggcatgttc cgtggcctcg
      301 cccaggccga cggcaccctc atcacatgtg tggattctgg gattctcaga gtctggcatg
      361 acaaggacaa ggacacatcc tctgacccac tcctggaact gagagtgggc cctggggtgt
      421 gtaggatgcg ccaagaccca gcacaccccc atgtggttgc cacaggtggg aaagagaatg
      481 ctttgaagat atgggacctg cagggctctg aggaacctgt gttcagggcc aagaacgtgc
      541 ggaatgactg gctggacttg cgggttccca tctgggacca ggacatacag tttctcccag
      601 gatcacagaa gcttgtcacc tgcacagggt accaccaggt ccgtgtttat gatccagcat
      661 ccccccagcg ccggccagtc ctagagacca cctatggaga gtacccacta acagccatga
      721 ccctcactcc gggaggcaac tcagtgattg tgggaaacac tcatgggcag ctggcagaaa
      781 ttgaccttcg gcaagggcgt ctactgggct gtctgaaggg gctggcaggc agtgtgcgtg
      841 ggttgcagtg ccacccttca aagcctctac tagcctcctg tggcttggac agagtcttga
      901 ggatacacag gatccagaat ccacggggtc tggagcataa ggtttatctc aagtctcaat
      961 tgaactgcct cctcttgtca ggcagggaca actgggagga tgagccccaa gagcctcaag
     1021 aacccaacaa ggtgccccta gaagacacag agacagatga actttgggca tccttggagg
     1081 cagctgccaa gcggaagctc tcgggtttgg agcagcccca aggagctctc caaacgagac
     1141 ggagaaagaa gaagcggcct gggtccacca gcccctgacg cccctgtgcc cactttgtaa
     1201 ataaactgct gaacaccca
//
