LOCUS       NM_032118               1147 bp    mRNA    linear   PRI 12-FEB-2025
DEFINITION  Homo sapiens WD repeat domain 54 (WDR54), transcript variant 3,
            mRNA.
ACCESSION   NM_032118
VERSION     NM_032118.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1147)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   2  (bases 1 to 1147)
  AUTHORS   Yuan,Y., Qi,G., Shen,H., Guo,A., Cao,F., Zhu,Y., Xiao,C., Chang,W.
            and Zheng,S.
  TITLE     Clinical significance and biological function of WD repeat domain
            54 as an oncogene in colorectal cancer
  JOURNAL   Int J Cancer 144 (7), 1584-1595 (2019)
   PUBMED   29987896
  REMARK    GeneRIF: WDR54 was found to be elevated and associated with
            prognosis in patients with colorectal cancer (CRC). Functional
            assays showed that WDR54 facilitates the aggressiveness of CRC
            cells and promotes tumor growth in mice. Knockdown of WDR54
            sensitizes CRC cells to the proliferation-inhibitory effect of SHP2
            inhibitor, an effect involving their common function in regulating
            AKT and ERK signaling.
REFERENCE   3  (bases 1 to 1147)
  AUTHORS   Maeda,A., Nishino,T., Matsunaga,R., Yokoyama,A., Suga,H., Yagi,T.
            and Konishi,H.
  TITLE     Transglutaminase-mediated cross-linking of WDR54 regulates EGF
            receptor-signaling
  JOURNAL   Biochim Biophys Acta Mol Cell Res 1866 (2), 285-295 (2019)
   PUBMED   30458214
REFERENCE   4  (bases 1 to 1147)
  AUTHORS   Rush,J., Moritz,A., Lee,K.A., Guo,A., Goss,V.L., Spek,E.J.,
            Zhang,H., Zha,X.M., Polakiewicz,R.D. and Comb,M.J.
  TITLE     Immunoaffinity profiling of tyrosine phosphorylation in cancer
            cells
  JOURNAL   Nat Biotechnol 23 (1), 94-101 (2005)
   PUBMED   15592455
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK023015.1 and AI203710.1.
            
            On Nov 23, 2018 this sequence version replaced NM_032118.3.
            
            Transcript Variant: This variant (3) uses an alternate splice
            junction compared to variant 1. The resulting isoform (b) is
            shorter at the N-terminus compared to isoform a. Variants 2 and 3
            both encode the same isoform (b).
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK222696.1, SRR5189661.18544.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000348227.4/ ENSP00000006526.6
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1100              AK023015.1         1-1100
            1101-1147           AI203710.1         16-62               c
FEATURES             Location/Qualifiers
     source          1..1147
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2p13.1"
     gene            1..1147
                     /gene="WDR54"
                     /note="WD repeat domain 54"
                     /db_xref="GeneID:84058"
                     /db_xref="HGNC:HGNC:25770"
                     /db_xref="MIM:620826"
     exon            1..87
                     /gene="WDR54"
                     /inference="alignment:Splign:2.1.0"
     exon            88..310
                     /gene="WDR54"
                     /inference="alignment:Splign:2.1.0"
     CDS             89..1093
                     /gene="WDR54"
                     /note="isoform b is encoded by transcript variant 3; WD
                     repeat-containing protein 54"
                     /codon_start=1
                     /product="WD repeat-containing protein 54 isoform b"
                     /protein_id="NP_115494.1"
                     /db_xref="CCDS:CCDS1940.1"
                     /db_xref="GeneID:84058"
                     /db_xref="HGNC:HGNC:25770"
                     /db_xref="MIM:620826"
                     /translation="MFRWERSIPLRGSAAALCNNLSVLQLPARNLTYFGVVHGPSAQL
                     LSAAPEGVPLAQRQLHAKEGAGVSPPLITQVHWCVLPFRVLLVLTSHRGIQMYESNGY
                     TMVYWHALDSGDASPVQAVFARGIAASGHFICVGTWSGRVLVFDIPAKGPNIVLSEEL
                     AGHQMPITDIATEPAQGQDCVADMVTADDSGLLCVWRSGPEFTLLTRIPGFGVPCPSV
                     QLWQGIIAAGYGNGQVHLYEATTGNLHVQINAHARAICALDLASEVGKLLSAGEDTFV
                     HIWKLSRNPESGYIEVEHCHGECVADTQLCGARFCDSSGNSFAVTGYDLAEIRRFSSV
                     "
     exon            311..373
                     /gene="WDR54"
                     /inference="alignment:Splign:2.1.0"
     exon            374..440
                     /gene="WDR54"
                     /inference="alignment:Splign:2.1.0"
     exon            441..494
                     /gene="WDR54"
                     /inference="alignment:Splign:2.1.0"
     exon            495..622
                     /gene="WDR54"
                     /inference="alignment:Splign:2.1.0"
     exon            623..723
                     /gene="WDR54"
                     /inference="alignment:Splign:2.1.0"
     exon            724..886
                     /gene="WDR54"
                     /inference="alignment:Splign:2.1.0"
     exon            887..961
                     /gene="WDR54"
                     /inference="alignment:Splign:2.1.0"
     exon            962..1147
                     /gene="WDR54"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1126..1131
                     /regulatory_class="polyA_signal_sequence"
                     /gene="WDR54"
                     /note="hexamer: CATAAA"
     polyA_site      1147
                     /gene="WDR54"
                     /note="major polyA site"
ORIGIN      
        1 gtgcgtacgt gcgtcgtctc tatggtggcg gcggatttgg agggacccta cgaaccagga
       61 gtcaggcgag ccgatctggg gctgcaggat gttccgctgg gagcgctcca ttcccctgcg
      121 aggctcggcc gccgccctgt gcaacaacct cagtgtgctg cagctgccgg ctcgcaacct
      181 cacgtatttt ggcgtggttc atggaccaag cgcccagctt ctcagcgctg ctcctgaggg
      241 tgtgcccttg gcccagcgcc agctccacgc taaggagggt gctggagtga gtcccccact
      301 tatcactcag gtccactggt gtgtcctccc cttccgagtg ctgctggtac tcacctcaca
      361 tcgaggaata cagatgtacg agtccaatgg ctacaccatg gtctactggc atgcactgga
      421 ctctggagat gcctccccag tacaggctgt gtttgcccgg ggaattgctg ccagtggcca
      481 cttcatctgt gtgggaacgt ggtcaggccg ggtgctggtg tttgacatcc cagcaaaggg
      541 tcccaacatt gtactgagcg aggagctggc tgggcaccag atgccaatca cagacattgc
      601 caccgagcct gcccagggac aggattgtgt ggctgacatg gtgacggcag atgactcagg
      661 cttgctgtgt gtctggcggt cagggccaga attcacatta ttgacccgca ttccaggatt
      721 tggagttccg tgcccctctg tgcagctgtg gcaggggatc atagcagcag gctatgggaa
      781 cggacaagtg catctatatg aggccactac aggaaatcta catgtccaga tcaatgccca
      841 tgcccgggcc atctgcgccc tggacctggc ttctgaggtg ggcaagctac tctctgcagg
      901 tgaggacacc tttgtgcata tctggaagct gagcagaaac ccagagagtg gctacattga
      961 ggtggaacac tgtcatggtg agtgtgtcgc cgacacccag ctgtgtggtg ctcgattttg
     1021 tgattcctca ggcaactcct ttgctgtgac tggctatgac cttgcggaga tccggagatt
     1081 cagcagtgtg tgagaagagc agccttcctt tgtccctgtg gtattcataa agtacccgct
     1141 ccaccca
//
