LOCUS       NM_018669               2106 bp    mRNA    linear   PRI 11-FEB-2025
DEFINITION  Homo sapiens WDR4 tRNA N7-guanosine methyltransferase non-catalytic
            subunit (WDR4), transcript variant 1, mRNA.
ACCESSION   NM_018669 NM_033662
VERSION     NM_018669.6
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2106)
  AUTHORS   Burkhalter,M.D., Stiff,T., Maerz,L.D., Casar Tena,T., Wiese,H.,
            Gerhards,J., Sailer,S.A., Vu,L.A.T., Duong Phu,M., Donow,C.,
            Alupei,M., Iben,S., Groth,M., Wiese,S., Church,J.A., Jeggo,P.A. and
            Philipp,M.
  TITLE     Cilia defects upon loss of WDR4 are linked to proteasomal
            hyperactivity and ubiquitin shortage
  JOURNAL   Cell Death Dis 15 (9), 660 (2024)
   PUBMED   39251572
  REMARK    GeneRIF: Cilia defects upon loss of WDR4 are linked to proteasomal
            hyperactivity and ubiquitin shortage.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2106)
  AUTHORS   Fu,Y., Jiang,F., Zhang,X., Pan,Y., Xu,R., Liang,X., Wu,X., Li,X.,
            Lin,K., Shi,R., Zhang,X., Ferrandon,D., Liu,J., Pei,D., Wang,J. and
            Wang,T.
  TITLE     Perturbation of METTL1-mediated tRNA N7- methylguanosine
            modification induces senescence and aging
  JOURNAL   Nat Commun 15 (1), 5713 (2024)
   PUBMED   38977661
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2106)
  AUTHORS   Dong,R., Wang,C., Tang,B., Cheng,Y., Peng,X., Yang,X., Ni,B. and
            Li,J.
  TITLE     WDR4 promotes HCC pathogenesis through N7-methylguanosine by
            regulating and interacting with METTL1
  JOURNAL   Cell Signal 118, 111145 (2024)
   PUBMED   38493882
  REMARK    GeneRIF: WDR4 promotes HCC pathogenesis through
            N[7]-methylguanosine by regulating and interacting with METTL1.
REFERENCE   4  (bases 1 to 2106)
  AUTHORS   Weng,Q., Zhang,F. and Zheng,Q.
  TITLE     A comprehensive model for tRNA methylation modification studies
  JOURNAL   MedComm (2020) 4 (6), e402 (2023)
   PUBMED   37873513
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2106)
  AUTHORS   Singh,S., Yeat,N.Y., Wang,Y.T., Lin,S.Y., Kuo,I.Y., Wu,K.P.,
            Wang,W.J., Wang,W.C., Su,W.C., Wang,Y.C. and Chen,R.H.
  TITLE     PTPN23 ubiquitination by WDR4 suppresses EGFR and c-MET degradation
            to define a lung cancer therapeutic target
  JOURNAL   Cell Death Dis 14 (10), 671 (2023)
   PUBMED   37821451
  REMARK    GeneRIF: PTPN23 ubiquitination by WDR4 suppresses EGFR and c-MET
            degradation to define a lung cancer therapeutic target.
            Erratum:[Cell Death Dis. 2024 Jul 2;15(7):468. doi:
            10.1038/s41419-024-06747-x.. PMID: 38956428]
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2106)
  AUTHORS   Cornelis,M.C., Fornage,M., Foy,M., Xun,P., Gladyshev,V.N.,
            Morris,S., Chasman,D.I., Hu,F.B., Rimm,E.B., Kraft,P., Jordan,J.M.,
            Mozaffarian,D. and He,K.
  TITLE     Genome-wide association study of selenium concentrations
  JOURNAL   Hum Mol Genet 24 (5), 1469-1477 (2015)
   PUBMED   25343990
REFERENCE   7  (bases 1 to 2106)
  AUTHORS   Hu,Y.H., Warnatz,H.J., Vanhecke,D., Wagner,F., Fiebitz,A.,
            Thamm,S., Kahlem,P., Lehrach,H., Yaspo,M.L. and Janitz,M.
  TITLE     Cell array-based intracellular localization screening reveals novel
            functional features of human chromosome 21 proteins
  JOURNAL   BMC Genomics 7, 155 (2006)
   PUBMED   16780588
  REMARK    Publication Status: Online-Only
REFERENCE   8  (bases 1 to 2106)
  AUTHORS   Cartlidge,R.A., Knebel,A., Peggie,M., Alexandrov,A., Phizicky,E.M.
            and Cohen,P.
  TITLE     The tRNA methylase METTL1 is phosphorylated and inactivated by PKB
            and RSK in vitro and in cells
  JOURNAL   EMBO J 24 (9), 1696-1705 (2005)
   PUBMED   15861136
REFERENCE   9  (bases 1 to 2106)
  AUTHORS   Alexandrov,A., Martzen,M.R. and Phizicky,E.M.
  TITLE     Two proteins that form a complex are required for 7-methylguanosine
            modification of yeast tRNA
  JOURNAL   RNA 8 (10), 1253-1266 (2002)
   PUBMED   12403464
REFERENCE   10 (bases 1 to 2106)
  AUTHORS   Michaud,J., Kudoh,J., Berry,A., Bonne-Tamir,B., Lalioti,M.D.,
            Rossier,C., Shibuya,K., Kawasaki,K., Asakawa,S., Minoshima,S.,
            Shimizu,N., Antonarakis,S.E. and Scott,H.S.
  TITLE     Isolation and characterization of a human chromosome 21q22.3 gene
            (WDR4) and its mouse homologue that code for a WD-repeat protein
  JOURNAL   Genomics 68 (1), 71-79 (2000)
   PUBMED   10950928
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AP001629.1 and AP001628.1.
            
            On May 17, 2019 this sequence version replaced NM_018669.5.
            
            Summary: This gene encodes a member of the WD repeat protein
            family. WD repeats are minimally conserved regions of approximately
            40 amino acids typically bracketed by gly-his and trp-asp (GH-WD),
            which may facilitate formation of heterotrimeric or multiprotein
            complexes. Members of this family are involved in a variety of
            cellular processes, including cell cycle progression, signal
            transduction, apoptosis, and gene regulation. This gene is excluded
            as a candidate for a form of nonsyndromic deafness (DFNB10), but is
            still a candidate for other disorders mapped to 21q22.3 as well as
            for the development of Down syndrome phenotypes. Alternatively
            spliced transcript variants have been found for this gene.
            [provided by RefSeq, May 2012].
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (1). Variants 1 and 2 both encode the same isoform.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC006341.2, SRR1803612.220992.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed/partial sample support
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000350]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000398208.3/ ENSP00000381266.2
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-133               AP001629.1         29404-29536         c
            134-199             AP001629.1         26699-26764         c
            200-340             AP001629.1         23548-23688         c
            341-497             AP001629.1         13437-13593         c
            498-610             AP001629.1         12279-12391         c
            611-671             AP001629.1         9659-9719           c
            672-770             AP001629.1         5679-5777           c
            771-835             AP001629.1         4559-4623           c
            836-1019            AP001629.1         3566-3749           c
            1020-1089           AP001629.1         2252-2321           c
            1090-1328           AP001629.1         1-239               c
            1329-2106           AP001628.1         105921-106698       c
FEATURES             Location/Qualifiers
     source          1..2106
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="21"
                     /map="21q22.3"
     gene            1..2106
                     /gene="WDR4"
                     /gene_synonym="GAMOS6; hWH; MIGSB; TRM82; TRMT82; Wuho"
                     /note="WDR4 tRNA N7-guanosine methyltransferase
                     non-catalytic subunit"
                     /db_xref="GeneID:10785"
                     /db_xref="HGNC:HGNC:12756"
                     /db_xref="MIM:605924"
     exon            1..133
                     /gene="WDR4"
                     /gene_synonym="GAMOS6; hWH; MIGSB; TRM82; TRMT82; Wuho"
                     /inference="alignment:Splign:2.1.0"
     CDS             45..1283
                     /gene="WDR4"
                     /gene_synonym="GAMOS6; hWH; MIGSB; TRM82; TRMT82; Wuho"
                     /note="isoform 1 is encoded by transcript variant 1; WD
                     repeat-containing protein 4; tRNA
                     (guanine-N(7)-)-methyltransferase subunit WDR4; TRM82 tRNA
                     methyltransferase 82 homolog; WD repeat domain 4; WD
                     repeat domain 4 METTL1 scaffold protein; tRNA
                     (guanine-N(7)-)-methyltransferase non-catalytic subunit
                     WDR4; protein Wuho homolog"
                     /codon_start=1
                     /product="tRNA (guanine-N(7)-)-methyltransferase
                     non-catalytic subunit WDR4 isoform 1"
                     /protein_id="NP_061139.2"
                     /db_xref="CCDS:CCDS13691.1"
                     /db_xref="GeneID:10785"
                     /db_xref="HGNC:HGNC:12756"
                     /db_xref="MIM:605924"
                     /translation="MAGSVGLALCGQTLVVRGGSRFLATSIASSDDDSLFIYDCSAAE
                     KKSQENKGEDAPLDQGSGAILASTFSKSGSYFALTDDSKRLILFRTKPWQCLSVRTVA
                     RRCTALTFIASEEKVLVADKSGDVYSFSVLEPHGCGRLELGHLSMLLDVAVSPDDRFI
                     LTADRDEKIRVSWAAAPHSIESFCLGHTEFVSRISVVPTQPGLLLSSSGDGTLRLWEY
                     RSGRQLHCCHLASLQELVDPQAPQKFAASRIAFWCQENCVALLCDGTPVVYIFQLDAR
                     RQQLVYRQQLAFQHQVWDVAFEETQGLWVLQDCQEAPLVLYRPVGDQWQSVPESTVLK
                     KVSGVLRGNWAMLEGSAGADASFSSLYKATFDNVTSYLKKKEERLQQQLEKKQRRRSP
                     PPGPDGHAKKMRPGEATLSC"
     exon            134..199
                     /gene="WDR4"
                     /gene_synonym="GAMOS6; hWH; MIGSB; TRM82; TRMT82; Wuho"
                     /inference="alignment:Splign:2.1.0"
     exon            200..340
                     /gene="WDR4"
                     /gene_synonym="GAMOS6; hWH; MIGSB; TRM82; TRMT82; Wuho"
                     /inference="alignment:Splign:2.1.0"
     exon            341..497
                     /gene="WDR4"
                     /gene_synonym="GAMOS6; hWH; MIGSB; TRM82; TRMT82; Wuho"
                     /inference="alignment:Splign:2.1.0"
     exon            498..610
                     /gene="WDR4"
                     /gene_synonym="GAMOS6; hWH; MIGSB; TRM82; TRMT82; Wuho"
                     /inference="alignment:Splign:2.1.0"
     exon            611..671
                     /gene="WDR4"
                     /gene_synonym="GAMOS6; hWH; MIGSB; TRM82; TRMT82; Wuho"
                     /inference="alignment:Splign:2.1.0"
     exon            672..770
                     /gene="WDR4"
                     /gene_synonym="GAMOS6; hWH; MIGSB; TRM82; TRMT82; Wuho"
                     /inference="alignment:Splign:2.1.0"
     exon            771..835
                     /gene="WDR4"
                     /gene_synonym="GAMOS6; hWH; MIGSB; TRM82; TRMT82; Wuho"
                     /inference="alignment:Splign:2.1.0"
     exon            836..1019
                     /gene="WDR4"
                     /gene_synonym="GAMOS6; hWH; MIGSB; TRM82; TRMT82; Wuho"
                     /inference="alignment:Splign:2.1.0"
     exon            1020..1089
                     /gene="WDR4"
                     /gene_synonym="GAMOS6; hWH; MIGSB; TRM82; TRMT82; Wuho"
                     /inference="alignment:Splign:2.1.0"
     exon            1090..2106
                     /gene="WDR4"
                     /gene_synonym="GAMOS6; hWH; MIGSB; TRM82; TRMT82; Wuho"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2088..2093
                     /regulatory_class="polyA_signal_sequence"
                     /gene="WDR4"
                     /gene_synonym="GAMOS6; hWH; MIGSB; TRM82; TRMT82; Wuho"
                     /note="hexamer: AATATA"
     polyA_site      2106
                     /gene="WDR4"
                     /gene_synonym="GAMOS6; hWH; MIGSB; TRM82; TRMT82; Wuho"
                     /note="major polyA site"
ORIGIN      
        1 gctctgggct ggcacatgtg tatggcggtg aggcgggcgg gtacatggcg ggctctgtgg
       61 gactggcgtt gtgcgggcag acgttggtgg tgcggggcgg cagccgattc ctggccacct
      121 ccatagcaag cagtgatgat gacagcctct tcatctatga ctgcagtgct gcagaaaaga
      181 agtcacaaga aaataaaggg gaggacgcgc ccttggacca ggggagcggt gcgattctgg
      241 cgtccacctt ctccaagtct ggcagctatt ttgctttaac cgatgacagt aagcgtctga
      301 ttcttttccg tacaaaacca tggcaatgtc tgagtgtcag gaccgtggca aggaggtgta
      361 cagccctgac tttcatagcc tcggaggaga aggtcttggt ggccgacaag tctggagacg
      421 tctactcctt ttcggtgctg gagccacacg ggtgtggccg tctagagctg gggcacctgt
      481 ctatgctgtt agatgtggct gtgagtcctg atgaccgctt catcctcact gccgaccggg
      541 acgagaagat ccgagtcagc tgggccgcgg cgccccatag catcgagtcc ttctgcttgg
      601 ggcacacaga gtttgtgagc cgtatctccg tggtgccaac tcagcccggg ctgcttctgt
      661 cctcctctgg ggacggcacc ctgaggctct gggagtacag gagcggccgc cagctgcact
      721 gctgtcacct ggccagtctg caggagctgg tggaccccca ggccccccag aagtttgccg
      781 cgtccaggat tgcattctgg tgccaggaga actgcgtggc gctcctgtgc gacggcactc
      841 ctgtggtcta catcttccag ctggacgccc gcagacagca gttggtgtac aggcagcagc
      901 tggcgttcca gcaccaagtg tgggacgtgg ctttcgagga gacccagggg ctgtgggtgc
      961 tccaggactg ccaggaagcc cccctggtgc tctacaggcc tgtgggcgac cagtggcagt
     1021 ctgttcctga gagcaccgtg ttaaagaaag tctctggtgt tcttcgtggg aactgggcca
     1081 tgctggaagg ctctgccggc gcagacgcca gcttcagcag tctctacaag gccacgttcg
     1141 acaacgtgac ctcctacctg aagaagaaag aggagagact gcagcagcag ctagagaaga
     1201 agcagcggcg ccggagtccc ccgcctgggc ccgacgggca tgccaagaag atgagaccgg
     1261 gggaggcgac gctaagttgc tgatcgtggc ggtcagtggt gtctctcacc tcaaattagg
     1321 aaaagctttt taacttgttc ccctgcatcc tggcatgtcc cttcaagaag gaaaaggtga
     1381 catcagttgt gaccagctca gaggatgggg ctgtcccctg ggctctagaa agaacattct
     1441 gtgtcaccag cacaggctct tgctgggtgc ctgtgccccc tttctggttg aagggaagcc
     1501 agaccagtgg agtggaagct cttgaagcac tttcttttca tgtcggaggc tgcgtgtgag
     1561 ccggtggtgg cggcagctgt ctgtgtttct cctgttctca taccgccctt cgtgtccgct
     1621 gcatcttcct ctaggagcat ctgtgggctg acgtttctgg catggtggat gttttcatgt
     1681 tgttccgccg tctcagcatc caggtgacgt gctgtcccgc agtagctgct cgccacacag
     1741 gctgttgaaa ctcagcacga atgcttgagc ctgccccagc cccagccgta gcccttgtgc
     1801 tcaggagctg ctcctggaga gtggctccct tgtgggaagt gagaaacacg tttcggagtc
     1861 gcagggagct ctgtctggca tcggtggcac agatggtgaa cttgtttcag gaagtgtgag
     1921 cacgggcctg gctggcaccc ggtttacagg gaggtgattg aggacgtcgt tatgatttga
     1981 tttgaccttg gcacacactg cagagccaca gggatgttgc tgaatttcaa tcaccgatca
     2041 gcaaggatga accttctcaa cttcctagga atgtttagca gcaaccgaat ataccaggca
     2101 aaactc
//
