LOCUS       NM_144574               2384 bp    mRNA    linear   PRI 10-DEC-2024
DEFINITION  Homo sapiens WD repeat domain 20 (WDR20), transcript variant 2,
            mRNA.
ACCESSION   NM_144574
VERSION     NM_144574.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2384)
  AUTHORS   Jiang,L., Qi,X., Lai,M., Zhou,J., Yuan,M., You,J., Liu,Q., Pan,J.,
            Zhao,L., Ying,M., Ji,J., Li,K., Zhang,Y., Pan,W., He,Q., Yang,B.
            and Cao,J.
  TITLE     WDR20 prevents hepatocellular carcinoma senescence by orchestrating
            the simultaneous USP12/46-mediated deubiquitination of c-Myc
  JOURNAL   Proc Natl Acad Sci U S A 121 (44), e2407904121 (2024)
   PUBMED   39432777
  REMARK    GeneRIF: WDR20 prevents hepatocellular carcinoma senescence by
            orchestrating the simultaneous USP12/46-mediated deubiquitination
            of c-Myc.
REFERENCE   2  (bases 1 to 2384)
  AUTHORS   Olazabal-Herrero,A., Bilbao-Arribas,M., Carlevaris,O., Sendino,M.,
            Varela-Martinez,E., Jugo,B.M., Berra,E. and Rodriguez,J.A.
  TITLE     The dystrophia myotonica WD repeat-containing protein DMWD and
            WDR20 differentially regulate USP12 deubiquitinase
  JOURNAL   FEBS J 288 (20), 5943-5963 (2021)
   PUBMED   33844468
REFERENCE   3  (bases 1 to 2384)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   4  (bases 1 to 2384)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   5  (bases 1 to 2384)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   6  (bases 1 to 2384)
  AUTHORS   Grossmann,A., Benlasfer,N., Birth,P., Hegele,A., Wachsmuth,F.,
            Apelt,L. and Stelzl,U.
  TITLE     Phospho-tyrosine dependent protein-protein interaction network
  JOURNAL   Mol Syst Biol 11 (3), 794 (2015)
   PUBMED   25814554
  REMARK    Publication Status: Online-Only
REFERENCE   7  (bases 1 to 2384)
  AUTHORS   Dahlberg,C.L. and Juo,P.
  TITLE     The WD40-repeat proteins WDR-20 and WDR-48 bind and activate the
            deubiquitinating enzyme USP-46 to promote the abundance of the
            glutamate receptor GLR-1 in the ventral nerve cord of
            Caenorhabditis elegans
  JOURNAL   J Biol Chem 289 (6), 3444-3456 (2014)
   PUBMED   24356955
REFERENCE   8  (bases 1 to 2384)
  AUTHORS   Kee,Y., Yang,K., Cohn,M.A., Haas,W., Gygi,S.P. and D'Andrea,A.D.
  TITLE     WDR20 regulates activity of the USP12 x UAF1 deubiquitinating
            enzyme complex
  JOURNAL   J Biol Chem 285 (15), 11252-11257 (2010)
   PUBMED   20147737
  REMARK    GeneRIF: WDR20 serves as a stimulatory subunit for preserving and
            regulating the activity of the subset of the UAF1 x USP12 complexes
REFERENCE   9  (bases 1 to 2384)
  AUTHORS   Sowa,M.E., Bennett,E.J., Gygi,S.P. and Harper,J.W.
  TITLE     Defining the human deubiquitinating enzyme interaction landscape
  JOURNAL   Cell 138 (2), 389-403 (2009)
   PUBMED   19615732
REFERENCE   10 (bases 1 to 2384)
  AUTHORS   Smith,T.F.
  TITLE     Diversity of WD-repeat proteins
  JOURNAL   Subcell Biochem 48, 20-30 (2008)
   PUBMED   18925368
  REMARK    Review article
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BC028387.2 and AL832242.1.
            
            On Jun 2, 2019 this sequence version replaced NM_144574.3.
            
            Summary: This gene encodes a WD repeat-containing protein that
            functions to preserve and regulate the activity of the USP12-UAF1
            deubiquitinating enzyme complex. Multiple alternatively spliced
            transcript variants have been found for this gene. [provided by
            RefSeq, Jun 2011].
            
            Transcript Variant: This variant (2) differs in the 3' coding
            region and 3' UTR, compared to variant 1. The encoded isoform (2)
            has a distinct and shorter C-terminus, compared to isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR3476690.1094183.1,
                                           SRR1803611.90174.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000342702.8/ ENSP00000341037.3
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-502               BC028387.2         5-506
            503-2381            AL832242.1         317-2195
            2382-2384           BC028387.2         2386-2388
FEATURES             Location/Qualifiers
     source          1..2384
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="14"
                     /map="14q32.31"
     gene            1..2384
                     /gene="WDR20"
                     /gene_synonym="Bun107; DMR"
                     /note="WD repeat domain 20"
                     /db_xref="GeneID:91833"
                     /db_xref="HGNC:HGNC:19667"
                     /db_xref="MIM:617741"
     exon            1..276
                     /gene="WDR20"
                     /gene_synonym="Bun107; DMR"
                     /inference="alignment:Splign:2.1.0"
     CDS             28..1737
                     /gene="WDR20"
                     /gene_synonym="Bun107; DMR"
                     /note="isoform 2 is encoded by transcript variant 2; WD
                     repeat-containing protein 20"
                     /codon_start=1
                     /product="WD repeat-containing protein 20 isoform 2"
                     /protein_id="NP_653175.2"
                     /db_xref="CCDS:CCDS9969.1"
                     /db_xref="GeneID:91833"
                     /db_xref="HGNC:HGNC:19667"
                     /db_xref="MIM:617741"
                     /translation="MATEGGGKEMNEIKTQFTTREGLYKLLPHSEYSRPNRVPFNSQG
                     SNPVRVSFVNLNDQSGNGDRLCFNVGRELYFYIYKGVRKAADLSKPIDKRIYKGTQPT
                     CHDFNHLTATAESVSLLVGFSAGQVQLIDPIKKETSKLFNEERLIDKSRVTCVKWVPG
                     SESLFLVAHSSGNMYLYNVEHTCGTTAPHYQLLKQGESFAVHTCKSKSTRNPLLKWTV
                     GEGALNEFAFSPDGKFLACVSQDGFLRVFNFDSVELHGTMKSYFGGLLCVCWSPDGKY
                     IVTGGEDDLVTVWSFVDCRVIARGHGHKSWVSVVAFDPYTTSVEEGDPMEFSGSDEDF
                     QDLLHFGRDRANSTQSRLSKRNSTDSRPVSVTYRFGSVGQDTQLCLWDLTEDILFPHQ
                     PLSRARTHTNVMNATSPPAGSNGNSVTTPGNSVPPPLPRSNSLPHSAVSNAGSKSSVM
                     DGAIASGVSKFATLSLHDRKERHHEKDHKRNHSMGHISSKSSDKLNLVTKTKTDPAKT
                     LGTPLCPRMEDVPLLEPLICKKIAHERLTVLIFLEDCIVTACQEGFICTWGRPGKVVS
                     FNP"
     exon            277..459
                     /gene="WDR20"
                     /gene_synonym="Bun107; DMR"
                     /inference="alignment:Splign:2.1.0"
     exon            460..2384
                     /gene="WDR20"
                     /gene_synonym="Bun107; DMR"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2362..2367
                     /regulatory_class="polyA_signal_sequence"
                     /gene="WDR20"
                     /gene_synonym="Bun107; DMR"
                     /note="hexamer: AATAAA"
     polyA_site      2384
                     /gene="WDR20"
                     /gene_synonym="Bun107; DMR"
                     /note="major polyA site"
ORIGIN      
        1 agggcaggat gaacgctgct ttccaagatg gcgacggagg gaggagggaa ggagatgaac
       61 gagattaaga cccaattcac cacccgggaa ggtctgtaca agctgctgcc gcactcggag
      121 tacagccggc ccaaccgggt gcccttcaac tcgcagggat ccaaccctgt ccgcgtctcc
      181 ttcgtaaacc tcaacgacca gtctggcaac ggcgaccgcc tctgcttcaa tgtgggccgg
      241 gagctgtact tctatatcta caagggggtc cgcaaggctg ctgacttgag taaaccaata
      301 gataaaagga tatacaaagg aacacagcct acttgtcatg acttcaacca cctaacagcc
      361 acagcagaaa gtgtctctct cctagtgggc ttttccgcag gccaagtcca gcttatagac
      421 ccaatcaaaa aagaaactag caaacttttt aatgaggaaa gactaataga caagtcacga
      481 gttacctgtg tcaaatgggt tcccggttcg gaaagccttt tcctagtagc ccactcgagt
      541 gggaacatgt acttatataa tgtggagcac acttgtggca ccacagcccc ccactaccag
      601 cttctgaagc agggagagag ctttgccgtg cacacttgca agagcaaatc cacgaggaac
      661 cctctcctta agtggacggt gggcgagggg gccctcaacg agtttgcttt ctccccagat
      721 ggcaagttct tagcgtgcgt gagccaggac gggtttctgc gggtgttcaa ctttgactca
      781 gtggagctgc acggtacgat gaaaagctac tttgggggct tgctgtgtgt gtgctggagc
      841 ccggatggca agtacatcgt gacaggtggg gaggacgact tggtgacagt ctggtccttt
      901 gtagactgcc gagtaatagc cagaggccac gggcacaagt cctgggtcag tgttgtagcg
      961 tttgaccctt ataccactag tgtagaagaa ggtgacccta tggagtttag tggcagcgat
     1021 gaggacttcc aagaccttct tcattttggc agagatcgag caaatagtac acagtccagg
     1081 ctctccaaac ggaactctac agacagccgc cccgtaagtg tcacgtatcg gtttggttcc
     1141 gtgggccagg acacacagct ctgtttatgg gaccttacag aagatatcct tttccctcac
     1201 caacccctct caagagcaag gacacacaca aatgtcatga atgccacgag tcctcctgct
     1261 ggaagcaatg ggaacagtgt tacaacaccc gggaactctg tgccgcctcc tctgccacgg
     1321 tccaacagcc ttccacattc agcagtctca aatgctggca gcaaaagcag tgtcatggac
     1381 ggggccattg cttctggggt cagcaaattt gcaacacttt cactacatga ccggaaggag
     1441 aggcaccacg agaaagatca caagcgaaat catagcatgg gacacatttc tagcaagagc
     1501 agtgacaaac tgaatctagt taccaaaacc aaaacggacc ctgctaaaac tctgggaacg
     1561 cccctgtgtc ctcgaatgga agatgttccc ttgttagagc cgctgatatg taaaaagata
     1621 gcacatgaga gactgactgt actaatattt cttgaagact gtatagtcac tgcttgtcag
     1681 gagggattta tttgcacatg gggaaggcct ggtaaagtgg taagttttaa tccttaatgc
     1741 tgcaccagat ctagaacttg aataggtagt gacttttttc tttttcgtgg gaggggtggg
     1801 gtgtacaatg aatgtgaatg acacttctta ttcttaatgt aaatctcaat gcatcagagc
     1861 cataattttg gatactgcat gccatgtaat tctgaatcat ttgataattt accttagagc
     1921 atttaaaaaa atataatcaa actaattgcc agccaagtca gtcatcctcc tgggagtata
     1981 tagagtccca aggttagcgc tcctgtatta gactatttca attttaggaa aatcatgacc
     2041 atgtggggaa acaatgactt taaaatgctg aaattaaaat ttatgcttta actggaatat
     2101 tttttgctta actactcaat tagaatattg tacacctgat caatgtgtgt tcagcacaga
     2161 tggccatgaa ttgtcattta tagtccaatt ttttatctta atcataaaat gtttaggaat
     2221 ctatgaaatt taactttagg aacaaaacgt ttagcagggt tgattgatat tatttttaca
     2281 ttgttctggc aatccacaga aagagaagag ccttaatttt taaaacccat tttagtcatt
     2341 ttatgacaat taaagttgtt taataaacat cttttttcaa agaa
//
