LOCUS       NM_003941               4363 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens WASP like actin nucleation promoting factor (WASL),
            mRNA.
ACCESSION   NM_003941
VERSION     NM_003941.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4363)
  AUTHORS   Cerutti,C., Lucotti,S., Menendez,S.T., Reymond,N., Garg,R.,
            Romero,I.A., Muschel,R. and Ridley,A.J.
  TITLE     IQGAP1 and NWASP promote human cancer cell dissemination and
            metastasis by regulating beta1-integrin via FAK and MRTF/SRF
  JOURNAL   Cell Rep 43 (4), 113989 (2024)
   PUBMED   38536816
  REMARK    GeneRIF: IQGAP1 and NWASP promote human cancer cell dissemination
            and metastasis by regulating beta1-integrin via FAK and MRTF/SRF.
REFERENCE   2  (bases 1 to 4363)
  AUTHORS   Kim,H.S., Lee,Y.S., Dong,S.M., Kim,H.J., Lee,D.E., Kang,H.W.,
            Kim,M.J. and Park,J.S.
  TITLE     Neural Wiskott-Aldrich syndrome protein (N-WASP) promotes distant
            metastasis in pancreatic ductal adenocarcinoma via activation of
            LOXL2
  JOURNAL   Oncol Res 32 (4), 615-624 (2024)
   PUBMED   38560567
  REMARK    GeneRIF: Neural Wiskott-Aldrich syndrome protein (N-WASP) promotes
            distant metastasis in pancreatic ductal adenocarcinoma via
            activation of LOXL2.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4363)
  AUTHORS   Yuen,W.S., Zhang,Q.H., Bourdais,A., Adhikari,D., Halet,G. and
            Carroll,J.
  TITLE     Polo-like kinase 1 promotes Cdc42-induced actin polymerization for
            asymmetric division in oocytes
  JOURNAL   Open Biol 13 (3), 220326 (2023)
   PUBMED   36883283
  REMARK    GeneRIF: Polo-like kinase 1 promotes Cdc42-induced actin
            polymerization for asymmetric division in oocytes.
REFERENCE   4  (bases 1 to 4363)
  AUTHORS   Frugtniet,B.A., Ruge,F., Sanders,A.J., Owen,S., Harding,K.G.,
            Jiang,W.G. and Martin,T.A.
  TITLE     nWASP Inhibition Increases Wound Healing via TrKb/PLCgamma
            Signalling
  JOURNAL   Biomolecules 13 (2), 379 (2023)
   PUBMED   36830748
  REMARK    GeneRIF: nWASP Inhibition Increases Wound Healing via TrKb/PLCgamma
            Signalling.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 4363)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   6  (bases 1 to 4363)
  AUTHORS   Qualmann,B., Roos,J., DiGregorio,P.J. and Kelly,R.B.
  TITLE     Syndapin I, a synaptic dynamin-binding protein that associates with
            the neural Wiskott-Aldrich syndrome protein
  JOURNAL   Mol Biol Cell 10 (2), 501-513 (1999)
   PUBMED   9950691
REFERENCE   7  (bases 1 to 4363)
  AUTHORS   Suetsugu,S., Miki,H. and Takenawa,T.
  TITLE     The essential role of profilin in the assembly of actin for
            microspike formation
  JOURNAL   EMBO J 17 (22), 6516-6526 (1998)
   PUBMED   9822597
REFERENCE   8  (bases 1 to 4363)
  AUTHORS   Suzuki,T., Miki,H., Takenawa,T. and Sasakawa,C.
  TITLE     Neural Wiskott-Aldrich syndrome protein is implicated in the
            actin-based motility of Shigella flexneri
  JOURNAL   EMBO J 17 (10), 2767-2776 (1998)
   PUBMED   9582270
REFERENCE   9  (bases 1 to 4363)
  AUTHORS   Miki,H., Sasaki,T., Takai,Y. and Takenawa,T.
  TITLE     Induction of filopodium formation by a WASP-related
            actin-depolymerizing protein N-WASP
  JOURNAL   Nature 391 (6662), 93-96 (1998)
   PUBMED   9422512
REFERENCE   10 (bases 1 to 4363)
  AUTHORS   Fukuoka,M., Miki,H. and Takenawa,T.
  TITLE     Identification of N-WASP homologs in human and rat brain
  JOURNAL   Gene 196 (1-2), 43-48 (1997)
   PUBMED   9322739
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BC052955.1, BC028602.1 and
            DB380642.1.
            
            On Nov 22, 2018 this sequence version replaced NM_003941.3.
            
            Summary: This gene encodes a member of the Wiskott-Aldrich syndrome
            (WAS) protein family. Wiskott-Aldrich syndrome proteins share
            similar domain structure, and associate with a variety of signaling
            molecules to alter the actin cytoskeleton. The encoded protein is
            highly expressed in neural tissues, and interacts with several
            proteins involved in cytoskeletal organization, including cell
            division control protein 42 (CDC42) and the actin-related
            protein-2/3 (ARP2/3) complex. The encoded protein may be involved
            in the formation of long actin microspikes, and in neurite
            extension. [provided by RefSeq, Jul 2013].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC052955.1, SRR1660805.240690.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000223023.5/ ENSP00000223023.4
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2793              BC052955.1         19-2811
            2794-4358           BC028602.1         2796-4360
            4359-4363           DB380642.1         17-21               c
FEATURES             Location/Qualifiers
     source          1..4363
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="7"
                     /map="7q31.32"
     gene            1..4363
                     /gene="WASL"
                     /gene_synonym="N-WASP; NWASP; WASPB"
                     /note="WASP like actin nucleation promoting factor"
                     /db_xref="GeneID:8976"
                     /db_xref="HGNC:HGNC:12735"
                     /db_xref="MIM:605056"
     exon            1..386
                     /gene="WASL"
                     /gene_synonym="N-WASP; NWASP; WASPB"
                     /inference="alignment:Splign:2.1.0"
     CDS             270..1787
                     /gene="WASL"
                     /gene_synonym="N-WASP; NWASP; WASPB"
                     /note="neural Wiskott-Aldrich syndrome protein;
                     Wiskott-Aldrich syndrome like"
                     /codon_start=1
                     /product="actin nucleation-promoting factor WASL"
                     /protein_id="NP_003932.3"
                     /db_xref="CCDS:CCDS34743.1"
                     /db_xref="GeneID:8976"
                     /db_xref="HGNC:HGNC:12735"
                     /db_xref="MIM:605056"
                     /translation="MSSVQQQPPPPRRVTNVGSLLLTPQENESLFTFLGKKCVTMSSA
                     VVQLYAADRNCMWSKKCSGVACLVKDNPQRSYFLRIFDIKDGKLLWEQELYNNFVYNS
                     PRGYFHTFAGDTCQVALNFANEEEAKKFRKAVTDLLGRRQRKSEKRRDPPNGPNLPMA
                     TVDIKNPEITTNRFYGPQVNNISHTKEKKKGKAKKKRLTKADIGTPSNFQHIGHVGWD
                     PNTGFDLNNLDPELKNLFDMCGISEAQLKDRETSKVIYDFIEKTGGVEAVKNELRRQA
                     PPPPPPSRGGPPPPPPPPHNSGPPPPPARGRGAPPPPPSRAPTAAPPPPPPSRPSVAV
                     PPPPPNRMYPPPPPALPSSAPSGPPPPPPSVLGVGPVAPPPPPPPPPPPGPPPPPGLP
                     SDGDHQVPTTAGNKAALLDQIREGAQLKKVEQNSRPVSCSGRDALLDQIRQGIQLKSV
                     ADGQESTPPTPAPTSGIVGALMEVMQKRSKAIHSSDEDEDEDDEEDFEDDDEWED"
     exon            387..521
                     /gene="WASL"
                     /gene_synonym="N-WASP; NWASP; WASPB"
                     /inference="alignment:Splign:2.1.0"
     exon            522..608
                     /gene="WASL"
                     /gene_synonym="N-WASP; NWASP; WASPB"
                     /inference="alignment:Splign:2.1.0"
     exon            609..705
                     /gene="WASL"
                     /gene_synonym="N-WASP; NWASP; WASPB"
                     /inference="alignment:Splign:2.1.0"
     exon            706..729
                     /gene="WASL"
                     /gene_synonym="N-WASP; NWASP; WASPB"
                     /inference="alignment:Splign:2.1.0"
     exon            730..898
                     /gene="WASL"
                     /gene_synonym="N-WASP; NWASP; WASPB"
                     /inference="alignment:Splign:2.1.0"
     exon            899..941
                     /gene="WASL"
                     /gene_synonym="N-WASP; NWASP; WASPB"
                     /inference="alignment:Splign:2.1.0"
     exon            942..1095
                     /gene="WASL"
                     /gene_synonym="N-WASP; NWASP; WASPB"
                     /inference="alignment:Splign:2.1.0"
     exon            1096..1616
                     /gene="WASL"
                     /gene_synonym="N-WASP; NWASP; WASPB"
                     /inference="alignment:Splign:2.1.0"
     exon            1617..1725
                     /gene="WASL"
                     /gene_synonym="N-WASP; NWASP; WASPB"
                     /inference="alignment:Splign:2.1.0"
     exon            1726..4363
                     /gene="WASL"
                     /gene_synonym="N-WASP; NWASP; WASPB"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4337..4342
                     /regulatory_class="polyA_signal_sequence"
                     /gene="WASL"
                     /gene_synonym="N-WASP; NWASP; WASPB"
                     /note="hexamer: AATAAA"
     polyA_site      4363
                     /gene="WASL"
                     /gene_synonym="N-WASP; NWASP; WASPB"
                     /note="major polyA site"
ORIGIN      
        1 gcagtcgccg gagtgcgagg acaacgacca tccggcccta gcctggccgg gcgggtgccg
       61 ggagcttccc tttctcagcg cggcgcgaag gtggctcgcc gtcagcgcct gcttccctcg
      121 acctcgtcct cctccccgct ccggaggagc tgcgagatgt ggcgcctctg actccacttc
      181 tccccgcccc tgtcaccgag agggggaacg agctctcgcc cactcgccgg aggagacggc
      241 cctggactcc caaccccgcc ggcgaaacca tgagctccgt ccagcagcag ccgccgccgc
      301 cgcggagggt caccaacgtg gggtccctgt tgctcacccc gcaggagaac gagtccctct
      361 tcactttcct cggcaagaaa tgtgtgacta tgtcttcagc agtggtgcag ttatatgcag
      421 cagatcggaa ctgtatgtgg tcaaagaagt gcagtggtgt tgcttgtctt gttaaggaca
      481 atccacagag atcttatttt ttaagaatat ttgacattaa ggatgggaaa ctattgtggg
      541 aacaagagct atacaataac tttgtatata atagtcctag aggatatttt catacctttg
      601 ctggagatac ttgtcaagtt gctcttaatt ttgccaatga agaagaagca aaaaaatttc
      661 gaaaagcagt tacagacctt ttgggccgtc gacaaaggaa atctgagaaa agacgagatc
      721 ccccaaatgg tcctaatcta cccatggcta cagttgatat aaaaaatcca gaaatcacaa
      781 caaatagatt ttatggtcca caagtcaaca acatctccca taccaaagaa aagaagaagg
      841 gaaaagctaa aaagaagaga ttaaccaagg cagatatagg aacaccaagc aatttccagc
      901 acattggaca tgttggttgg gatccaaata caggctttga tctgaataat ttggatccag
      961 aattgaagaa tcttttcgat atgtgtggaa tctcagaggc acaacttaaa gacagagaaa
     1021 catcaaaagt tatatatgac tttattgaaa aaacaggagg tgttgaagct gttaaaaatg
     1081 aactgcggag gcaagcacca ccacctccac caccatcaag gggagggcca cctcctcctc
     1141 ctccccctcc acacaactca ggtcctcctc ctcctcctgc taggggaaga ggcgctcctc
     1201 ccccaccacc ttcaagagct cccacagctg cacctccacc accgcctcct tccaggccaa
     1261 gtgtagcagt ccctccacca ccgccaaata ggatgtaccc tcctccacct ccagcccttc
     1321 cctcctcagc accttcaggg cctccaccac cacctccatc tgtgttgggg gtagggccag
     1381 tggcaccacc cccaccgcct ccacctccac ctcctcctgg gccaccgccc ccgcctggcc
     1441 tgccttctga tggggaccat caggttccaa ctactgcagg aaacaaagca gctcttttag
     1501 atcaaattag agagggtgct cagctaaaaa aagtggagca gaacagtcgg ccagtgtcct
     1561 gctctggacg agatgcactg ttagaccaga tacgacaggg tatccaacta aaatctgtgg
     1621 ctgatggcca agagtctaca ccaccaacac ctgcacccac ttcaggaatt gtgggtgcat
     1681 taatggaagt gatgcagaaa aggagcaaag ccattcattc ttcagatgaa gatgaagatg
     1741 aagatgatga agaagatttt gaggatgatg atgagtggga agactgatct atatattata
     1801 tatatatata tatttttaag gtgaaatact aaacactact ttctgtctgt ggattctgta
     1861 aaattatcat gtaaatgttc taccaatttg ctgtatattt ttgttgcctt ttttgctttt
     1921 ttttaatctg tgcaatacct catttaatct gtaaaggttt gtcgtaatcc tctacaaagc
     1981 tactccctgt tattgtgtgc aagtttacac caggatgctc acttgatttg tgaatatttt
     2041 tcattccatc aacaagggag tttaaatact atatgtgaga ctgacaaaaa ccttaatgta
     2101 atttacttat aatgccagaa ggaaaacact attttcatac cctacttttt ctgtacctaa
     2161 attttcttaa aaaaaaatct agtatagcac tacattcttt tttaagtgat gcagacctta
     2221 gtttctttag cccctttatt ttgaatacaa tgctacatat gaatgttgaa gctgatacat
     2281 tgcacagttc tgtagacatc actacaccga tgtagtttct caaattttag caatatgctc
     2341 tacataaaat cactacagag atactagtgg ggaagacgat taacacacct cttacagtaa
     2401 tactgcctgt tattggtata gcagtggtat ttgcagactg ggatcataag gagcccttaa
     2461 atacttgtta ttgactgggg ttatttttat gctgtagcaa atgtgacagg ctctttttag
     2521 caaaattttt gaaaattttt ttggtattac tctgaaacaa aatttaagtt ggagtttcag
     2581 ggatttaggg agtagttttc attctacatg aactgaggta atattatggt aactccaata
     2641 tttggttaaa aaaactatac aaatcagaat agtactaaaa tactgtagaa ttttagcatt
     2701 tttattttgc actttgtgtg gattgaggtg ttcagaaata ccaaccataa aaatgtaatc
     2761 tagttggcaa aggtgtgcgc taaaacacgg aaccgaacat gcattgattt ggataacttt
     2821 tgagggtttt tgtcaaatag catgtgaaga gttacatttt tcttaaaaga ttggtggtcc
     2881 caatgtcaga gttcttggaa cagataactg aatgatagat tttttttttt taaagataaa
     2941 actttacaac ctgcacattt gttatgcata ctaaatggtg tgttaaaatt agggtttctt
     3001 tgcctctcta cactacacta atctgcctaa aggtggttgt ttcatattta taatgctaat
     3061 tatcatacct acctacttta aattttaggt agaaaattat ctgatttaaa tacaaacata
     3121 tttttctcac attgagtaat atgcataatg tagttccaaa tgtatttcat tactatagtc
     3181 acaatatcca actaaaaatt acgctatcta gaattgtacc aaccaaaatc tcgtattggc
     3241 agatcttgac aggctggacc tgcaagatgt ggcttgaatt ttaaccattt attacataat
     3301 ctctagtgat catgcatcta gttatcataa gaaataattt aaaaggtttt gttgctgaaa
     3361 gcagtaagtg gtgcagtaaa atacttaagt tattcaaaga atgttatctt tcttgcaaga
     3421 gtaatttaag cacatgggaa agattctaga ctttttgttt cttgcaacaa cagtgccctc
     3481 tgctgctaga aacctttttc tacttactat catttttatt gtggcttgag ctcagtcaat
     3541 ctggtgcaga tgaggctgga caactactaa ccaataaaat caggagtttg tacaaaagtt
     3601 aaatggaaca ttataattat tttaagtaat gttaattgaa aaaatttttt ctttttgaca
     3661 atataaaaaa cattttaaat ttcctagaaa tgtcttcagg gtaatgccaa tttaagatct
     3721 ctcttaactt gtggccaaga aattctagat tttccagctg actttggtat aatatatatt
     3781 tatcaggaac ttctctcagc agagagtacc cattcttcga gttcaaagca caattttaaa
     3841 catgtaaaat ggatatataa tttgccaaag gtaattaatt tattcttttt ttcttaaaga
     3901 agaaaaagac attggttgtt gttacaaaaa gtctaaatta ctgttggttg aaacacagca
     3961 gctgtggagt tcagtccagg catgaagact aaatctgctt taccaagtaa ggtgtaaggt
     4021 tgcatgtttc agtcctctac catcctgcac tgtgagcagc actatacctg tgcgttgtct
     4081 gaaagccttc cgtatattct ccagctaaat ggtcgtctgg tagcctttgc atttaacaaa
     4141 ctagcatgag gctttttata tctaaatgct acgtgataga caatttcagc tagccacata
     4201 ttgtatgtat gagattcata aagactttca atcatttcat tttcagacaa ctgaaatttt
     4261 gtttagtatg tgaattcttt ttttgaaatg tatagtgaat aggatgttgc actggtggca
     4321 attcatcatg gagcataata aaattaattt gacccaaata gaa
//
