LOCUS       NM_014846               4139 bp    mRNA    linear   PRI 09-OCT-2024
DEFINITION  Homo sapiens WASH complex subunit 5 (WASHC5), transcript variant 1,
            mRNA.
ACCESSION   NM_014846
VERSION     NM_014846.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4139)
  AUTHORS   Neri,S., Maia,N., Fortuna,A.M., Damasio,J., Coale,E., Willis,M.,
            Jorge,P., Hojte,A.F., Fenger,C.D., Moller,R.S. and Bayat,A.
  TITLE     Expanding the pre- and postnatal phenotype of WASHC5 and CCDC22
            -related Ritscher-Schinzel syndromes
  JOURNAL   Eur J Med Genet 65 (11), 104624 (2022)
   PUBMED   36130690
  REMARK    GeneRIF: Expanding the pre- and postnatal phenotype of WASHC5 and
            CCDC22 -related Ritscher-Schinzel syndromes.
REFERENCE   2  (bases 1 to 4139)
  AUTHORS   Ginanneschi,F., D'Amore,A., Barghigiani,M., Tessa,A., Rossi,A. and
            Santorelli,F.M.
  TITLE     SPG8 mutations in Italian families: clinical data and literature
            review
  JOURNAL   Neurol Sci 41 (3), 699-703 (2020)
   PUBMED   31814071
  REMARK    GeneRIF: SPG8 mutations in Italian families: clinical data and
            literature review.
            Review article
REFERENCE   3  (bases 1 to 4139)
  AUTHORS   Li,G., Qing,Y., Yang,X., Lou,J., Hu,X., Yang,C., Zhang,J., He,L.,
            Li,J. and Wan,C.
  TITLE     [Analysis of KIAA0196 gene mutation in a family with hereditary
            spastic paraplegia]
  JOURNAL   Zhonghua Yi Xue Yi Chuan Xue Za Zhi 36 (6), 584-587 (2019)
   PUBMED   31055811
  REMARK    GeneRIF: clinical phenotype of the c.1771T>C mutation of KIAA0196
            has a considerable heterogeneity and this mutation may be a common
            pathogenic site of KIAA0196 mutations among Chinese patients with
            hereditary spastic paraplegia
REFERENCE   4  (bases 1 to 4139)
  AUTHORS   Ichinose,Y., Koh,K., Fukumoto,M., Yamashiro,N., Kobayashi,F.,
            Miwa,M., Nagasaka,T., Shindo,K., Ishiura,H., Tsuji,S. and
            Takiyama,D.Y.
  TITLE     Exome sequencing reveals a novel missense mutation in the KIAA0196
            gene in a Japanese patient with SPG8
  JOURNAL   Clin Neurol Neurosurg 144, 36-38 (2016)
   PUBMED   26967522
  REMARK    GeneRIF: A novel missense mutation was identified in the KIAA0196
            gene in a Japanese patient with SPG8.
REFERENCE   5  (bases 1 to 4139)
  AUTHORS   Porkka,K.P., Tammela,T.L., Vessella,R.L. and Visakorpi,T.
  TITLE     RAD21 and KIAA0196 at 8q24 are amplified and overexpressed in
            prostate cancer
  JOURNAL   Genes Chromosomes Cancer 39 (1), 1-10 (2004)
   PUBMED   14603436
  REMARK    GeneRIF: The expression of KIAA0196 at chromosomal region 8q24 is
            significantly higher in prostate carcinomas with gene amplification
            than in those without it.
REFERENCE   6  (bases 1 to 4139)
  AUTHORS   Gevaert,K., Goethals,M., Martens,L., Van Damme,J., Staes,A.,
            Thomas,G.R. and Vandekerckhove,J.
  TITLE     Exploring proteomes and analyzing protein processing by mass
            spectrometric identification of sorted N-terminal peptides
  JOURNAL   Nat Biotechnol 21 (5), 566-569 (2003)
   PUBMED   12665801
REFERENCE   7  (bases 1 to 4139)
  AUTHORS   Hedera,P., Rainier,S., Alvarado,D., Zhao,X., Williamson,J.,
            Otterud,B., Leppert,M. and Fink,J.K.
  TITLE     Novel locus for autosomal dominant hereditary spastic paraplegia,
            on chromosome 8q
  JOURNAL   Am J Hum Genet 64 (2), 563-569 (1999)
   PUBMED   9973294
REFERENCE   8  (bases 1 to 4139)
  AUTHORS   Elliott,A.M. and Chudley,A.
  TITLE     Ritscher-Schinzel Syndrome
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   31971710
REFERENCE   9  (bases 1 to 4139)
  AUTHORS   Meijer,I.A., Valdmanis,P.N. and Rouleau,G.A.
  TITLE     Spastic Paraplegia 8
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301727
REFERENCE   10 (bases 1 to 4139)
  AUTHORS   Hedera,P.
  TITLE     Hereditary Spastic Paraplegia Overview
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301682
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA185885.1, D83780.1,
            BC106015.1 and AI266595.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_014846.3.
            
            Summary: This gene encodes a 134 kDa protein named strumpellin that
            is predicted to have multiple transmembrane domains and a
            spectrin-repeat-containing domain. This ubiquitously expressed gene
            has its highest expression in skeletal muscle. The protein is named
            for Strumpell disease; a form of hereditary spastic paraplegia
            (HSP). Spastic paraplegias are a diverse group of disorders in
            which the autosomal dominant forms are characterized by
            progressive, lower extremity spasticity caused by axonal
            degeneration in the terminal portions of the longest descending and
            ascending corticospinal tracts. More than 30 loci (SPG1-33) have
            been implicated in hereditary spastic paraplegia diseases.
            [provided by RefSeq, Aug 2009].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038197.799210.1,
                                           SRR14038196.2607116.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000318410.12/ ENSP00000318016.7
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-29                DA185885.1         28-56
            30-1546             D83780.1           1-1517
            1547-4133           BC106015.1         1463-4049
            4134-4139           AI266595.1         8-13                c
FEATURES             Location/Qualifiers
     source          1..4139
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="8"
                     /map="8q24.13"
     gene            1..4139
                     /gene="WASHC5"
                     /gene_synonym="KIAA0196; RTSC; RTSC1; SPG8"
                     /note="WASH complex subunit 5"
                     /db_xref="GeneID:9897"
                     /db_xref="HGNC:HGNC:28984"
                     /db_xref="MIM:610657"
     exon            1..178
                     /gene="WASHC5"
                     /gene_synonym="KIAA0196; RTSC; RTSC1; SPG8"
                     /inference="alignment:Splign:2.1.0"
     exon            179..488
                     /gene="WASHC5"
                     /gene_synonym="KIAA0196; RTSC; RTSC1; SPG8"
                     /inference="alignment:Splign:2.1.0"
     CDS             303..3782
                     /gene="WASHC5"
                     /gene_synonym="KIAA0196; RTSC; RTSC1; SPG8"
                     /note="isoform 1 is encoded by transcript variant 1;
                     strumpellin; WASH complex subunit strumpellin"
                     /codon_start=1
                     /product="WASH complex subunit 5 isoform 1"
                     /protein_id="NP_055661.3"
                     /db_xref="CCDS:CCDS6355.1"
                     /db_xref="GeneID:9897"
                     /db_xref="HGNC:HGNC:28984"
                     /db_xref="MIM:610657"
                     /translation="MLDFLAENNLCGQAILRIVSCGNAIIAELLRLSEFIPAVFRLKD
                     RADQQKYGDIIFDFSYFKGPELWESKLDAKPELQDLDEEFRENNIEIVTRFYLAFQSV
                     HKYIVDLNRYLDDLNEGVYIQQTLETVLLNEDGKQLLCEALYLYGVMLLVIDQKIEGE
                     VRERMLVSYYRYSAARSSADSNMDDICKLLRSTGYSSQPGAKRPSNYPESYFQRVPIN
                     ESFISMVIGRLRSDDIYNQVSAYPLPEHRSTALANQAAMLYVILYFEPSILHTHQAKM
                     REIVDKYFPDNWVISIYMGITVNLVDAWEPYKAAKTALNNTLDLSNVREQASRYATVS
                     ERVHAQVQQFLKEGYLREEMVLDNIPKLLNCLRDCNVAIRWLMLHTADSACDPNNKRL
                     RQIKDQILTDSRYNPRILFQLLLDTAQFEFILKEMFKQMLSEKQTKWEHYKKEGSERM
                     TELADVFSGVKPLTRVEKNENLQAWFREISKQILSLNYDDSTAAGRKTVQLIQALEEV
                     QEFHQLESNLQVCQFLADTRKFLHQMIRTINIKEEVLITMQIVGDLSFAWQLIDSFTS
                     IMQESIRVNPSMVTKLRATFLKLASALDLPLLRINQANSPDLLSVSQYYSGELVSYVR
                     KVLQIIPESMFTSLLKIIKLQTHDIIEVPTRLDKDKLRDYAQLGPRYEVAKLTHAISI
                     FTEGILMMKTTLVGIIKVDPKQLLEDGIRKELVKRVAFALHRGLIFNPRAKPSELMPK
                     LKELGATMDGFHRSFEYIQDYVNIYGLKIWQEEVSRIINYNVEQECNNFLRTKIQDWQ
                     SMYQSTHIPIPKFTPVDESVTFIGRLCREILRITDPKMTCHIDQLNTWYDMKTHQEVT
                     SSRLFSEIQTTLGTFGLNGLDRLLCFMIVKELQNFLSMFQKIILRDRTVQDTLKTLMN
                     AVSPLKSIVANSNKIYFSAIAKTQKIWTAYLEAIMKVGQMQILRQQIANELNYSCRFD
                     SKHLAAALENLNKALLADIEAHYQDPSLPYPKEDNTLLYEITAYLEAAGIHNPLNKIY
                     ITTKRLPYFPIVNFLFLIAQLPKLQYNKNLGMVCRKPTDPVDWPPLVLGLLTLLKQFH
                     SRYTEQFLALIGQFICSTVEQCTSQKIPEIPADVVGALLFLEDYVRYTKLPRRVAEAH
                     VPNFIFDEFRTVL"
     exon            489..634
                     /gene="WASHC5"
                     /gene_synonym="KIAA0196; RTSC; RTSC1; SPG8"
                     /inference="alignment:Splign:2.1.0"
     exon            635..719
                     /gene="WASHC5"
                     /gene_synonym="KIAA0196; RTSC; RTSC1; SPG8"
                     /inference="alignment:Splign:2.1.0"
     exon            720..820
                     /gene="WASHC5"
                     /gene_synonym="KIAA0196; RTSC; RTSC1; SPG8"
                     /inference="alignment:Splign:2.1.0"
     exon            821..1013
                     /gene="WASHC5"
                     /gene_synonym="KIAA0196; RTSC; RTSC1; SPG8"
                     /inference="alignment:Splign:2.1.0"
     exon            1014..1166
                     /gene="WASHC5"
                     /gene_synonym="KIAA0196; RTSC; RTSC1; SPG8"
                     /inference="alignment:Splign:2.1.0"
     exon            1167..1280
                     /gene="WASHC5"
                     /gene_synonym="KIAA0196; RTSC; RTSC1; SPG8"
                     /inference="alignment:Splign:2.1.0"
     exon            1281..1452
                     /gene="WASHC5"
                     /gene_synonym="KIAA0196; RTSC; RTSC1; SPG8"
                     /inference="alignment:Splign:2.1.0"
     exon            1453..1580
                     /gene="WASHC5"
                     /gene_synonym="KIAA0196; RTSC; RTSC1; SPG8"
                     /inference="alignment:Splign:2.1.0"
     exon            1581..1710
                     /gene="WASHC5"
                     /gene_synonym="KIAA0196; RTSC; RTSC1; SPG8"
                     /inference="alignment:Splign:2.1.0"
     exon            1711..1823
                     /gene="WASHC5"
                     /gene_synonym="KIAA0196; RTSC; RTSC1; SPG8"
                     /inference="alignment:Splign:2.1.0"
     exon            1824..1990
                     /gene="WASHC5"
                     /gene_synonym="KIAA0196; RTSC; RTSC1; SPG8"
                     /inference="alignment:Splign:2.1.0"
     exon            1991..2066
                     /gene="WASHC5"
                     /gene_synonym="KIAA0196; RTSC; RTSC1; SPG8"
                     /inference="alignment:Splign:2.1.0"
     exon            2067..2177
                     /gene="WASHC5"
                     /gene_synonym="KIAA0196; RTSC; RTSC1; SPG8"
                     /inference="alignment:Splign:2.1.0"
     exon            2178..2318
                     /gene="WASHC5"
                     /gene_synonym="KIAA0196; RTSC; RTSC1; SPG8"
                     /inference="alignment:Splign:2.1.0"
     exon            2319..2399
                     /gene="WASHC5"
                     /gene_synonym="KIAA0196; RTSC; RTSC1; SPG8"
                     /inference="alignment:Splign:2.1.0"
     exon            2400..2501
                     /gene="WASHC5"
                     /gene_synonym="KIAA0196; RTSC; RTSC1; SPG8"
                     /inference="alignment:Splign:2.1.0"
     exon            2502..2681
                     /gene="WASHC5"
                     /gene_synonym="KIAA0196; RTSC; RTSC1; SPG8"
                     /inference="alignment:Splign:2.1.0"
     exon            2682..2806
                     /gene="WASHC5"
                     /gene_synonym="KIAA0196; RTSC; RTSC1; SPG8"
                     /inference="alignment:Splign:2.1.0"
     exon            2807..2969
                     /gene="WASHC5"
                     /gene_synonym="KIAA0196; RTSC; RTSC1; SPG8"
                     /inference="alignment:Splign:2.1.0"
     exon            2970..3072
                     /gene="WASHC5"
                     /gene_synonym="KIAA0196; RTSC; RTSC1; SPG8"
                     /inference="alignment:Splign:2.1.0"
     exon            3073..3152
                     /gene="WASHC5"
                     /gene_synonym="KIAA0196; RTSC; RTSC1; SPG8"
                     /inference="alignment:Splign:2.1.0"
     exon            3153..3256
                     /gene="WASHC5"
                     /gene_synonym="KIAA0196; RTSC; RTSC1; SPG8"
                     /inference="alignment:Splign:2.1.0"
     exon            3257..3386
                     /gene="WASHC5"
                     /gene_synonym="KIAA0196; RTSC; RTSC1; SPG8"
                     /inference="alignment:Splign:2.1.0"
     exon            3387..3483
                     /gene="WASHC5"
                     /gene_synonym="KIAA0196; RTSC; RTSC1; SPG8"
                     /inference="alignment:Splign:2.1.0"
     exon            3484..3637
                     /gene="WASHC5"
                     /gene_synonym="KIAA0196; RTSC; RTSC1; SPG8"
                     /inference="alignment:Splign:2.1.0"
     exon            3638..3725
                     /gene="WASHC5"
                     /gene_synonym="KIAA0196; RTSC; RTSC1; SPG8"
                     /inference="alignment:Splign:2.1.0"
     exon            3726..4139
                     /gene="WASHC5"
                     /gene_synonym="KIAA0196; RTSC; RTSC1; SPG8"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4113..4118
                     /regulatory_class="polyA_signal_sequence"
                     /gene="WASHC5"
                     /gene_synonym="KIAA0196; RTSC; RTSC1; SPG8"
                     /note="hexamer: AGTAAA"
     polyA_site      4139
                     /gene="WASHC5"
                     /gene_synonym="KIAA0196; RTSC; RTSC1; SPG8"
                     /note="major polyA site"
ORIGIN      
        1 gcttcggtca tgtgaccgcc tggggagtca ggggcggaag tcggggtctg acccgctcca
       61 ggtccgggac tgcggataga agaggaccgc cgccttgagg gaggggtgga aactgggtgc
      121 cggctccgcg cgcgacctcc ggccctgcgc gtgcgccgtg gcgcggcccg gctgacaggt
      181 tctttaatgg aggagccaat ctctctgcac acctggtttc atctaataat atacagacac
      241 cagctctgag gccagttaat catccccagt gtccaggcac agagtagtcg gtccgcctca
      301 caatgttgga ctttctagcc gagaacaacc tctgtggcca agcaatccta aggattgttt
      361 cctgtggtaa tgccatcatt gctgaacttt tgagactctc tgagtttatt cctgctgtgt
      421 tcaggttaaa agacagagct gatcaacaga aatatggaga tatcatattt gatttcagct
      481 attttaaggg tccagaatta tgggaaagca aactggatgc taagccagag ctacaggatt
      541 tagatgaaga atttcgtgaa aacaacatag aaattgtgac cagattttat ttagcatttc
      601 aaagtgtaca taaatatatt gtagacttaa acagatatct agatgatctc aatgaagggg
      661 tttatattca gcaaacctta gaaactgtgc ttctcaatga agatggaaaa caacttctat
      721 gtgaagcact gtacttatat ggagttatgc tactggtcat tgaccaaaag attgaaggag
      781 aagtcagaga gaggatgctg gtttcttact accgatacag tgctgctcga tcttctgctg
      841 attcaaatat ggacgatatt tgtaagctgc ttcgaagtac aggttattct agccaaccag
      901 gtgccaaaag accatccaac tatcccgaga gctatttcca gagagtgcct atcaacgaat
      961 ccttcatcag tatggtcatt ggtcgactga gatctgatga tatttacaac caggtctcag
     1021 cgtatccttt gccggagcat cgcagcacag ccctggcaaa ccaagctgcc atgctgtacg
     1081 tgattctcta ctttgagcct tccatccttc acacccatca agcaaaaatg agagagatag
     1141 tggataaata ctttccagat aattgggtaa ttagtattta catggggatc acagttaatc
     1201 tagtagatgc ttgggaacct tacaaagctg caaaaactgc tttaaataat accctggacc
     1261 tttcaaatgt cagagaacag gcaagcagat atgctactgt cagtgaaaga gtgcatgctc
     1321 aagtgcagca atttctaaaa gaaggttatt taagggagga gatggttctg gacaatatcc
     1381 caaagcttct gaactgcctg agagactgca atgttgccat ccgatggctg atgcttcata
     1441 cagcagactc agcctgtgac ccaaacaaca aacgccttcg tcaaatcaag gaccagattc
     1501 taacagactc tcggtacaat cccaggatcc tcttccagct gctgttagat actgcacaat
     1561 ttgagtttat actcaaagag atgttcaagc aaatgctttc agaaaagcaa accaaatggg
     1621 agcattacaa gaaagagggt tcggagcgga tgactgagct tgctgatgtc ttttcaggag
     1681 tgaaacccct aaccagagtg gagaaaaatg aaaaccttca agcttggttc agagagatct
     1741 caaaacaaat attgtcttta aattatgatg attctactgc tgcgggcaga aaaactgtac
     1801 aactgataca agctttggaa gaggttcaag aattccacca gttggaatcc aatctgcaag
     1861 tatgtcagtt tcttgccgat actcgaaagt ttcttcatca aatgatcaga accattaaca
     1921 ttaaagagga ggttctgatc acaatgcaga tcgttgggga cctttctttc gcttggcagt
     1981 tgattgacag tttcacatcc atcatgcaag aaagcataag ggtaaatcca tccatggtta
     2041 ctaaactcag agctaccttc ctaaagcttg cctctgccct cgatctgccc cttcttcgta
     2101 ttaatcaggc aaatagcccc gacctgctca gcgtgtcaca gtactattct ggagagttgg
     2161 tatcctatgt gagaaaagtt ttgcagatca tcccagaaag catgtttaca tctcttctaa
     2221 agatcataaa gcttcagacc cacgacatta ttgaagtgcc tacccgcctg gacaaagaca
     2281 agctgaggga ctatgctcag ctaggcccac gatacgaggt tgccaagctt actcatgcta
     2341 tttccatttt tactgaaggc atcttaatga tgaaaacgac tttggttggc atcatcaagg
     2401 tggatccaaa gcagttgctg gaagatggaa taaggaaaga gcttgtgaag cgcgttgcct
     2461 ttgccctgca taggggactg atattcaacc ctcgagccaa gccaagtgaa ttgatgccca
     2521 agctgaaaga gttgggagcg accatggatg gattccatcg ttcttttgaa tacatacagg
     2581 actatgtcaa catttatggt ctgaagattt ggcaggaaga agtatctcgt atcataaatt
     2641 acaacgtgga gcaagagtgt aataactttc taagaacgaa gattcaagat tggcaaagca
     2701 tgtaccagtc cactcatatt ccaataccca agtttacccc tgtggatgag tctgtaacgt
     2761 ttattggtcg actctgcaga gaaatcctgc ggatcacaga cccaaaaatg acatgtcaca
     2821 tagaccagct gaacacttgg tatgatatga aaactcatca ggaagtgacc agcagccgcc
     2881 tcttctcaga aatccagacc accttgggaa cctttggtct aaatggctta gacaggcttc
     2941 tgtgctttat gattgtaaaa gagttacaga atttcctcag tatgtttcag aaaattatcc
     3001 tgagagacag aactgttcag gacactttaa aaaccctcat gaatgctgtc agtcccctaa
     3061 aaagtattgt cgcaaattca aataaaattt atttttccgc cattgccaaa acacagaaga
     3121 tttggactgc gtatctcgag gctataatga aggttgggca gatgcagatt ctgagacaac
     3181 agattgccaa tgaattaaat tattcttgtc ggtttgattc taaacatctg gcagctgctc
     3241 tggagaatct caataaggct ctcctagcag acattgaagc ccactatcag gacccttcac
     3301 ttccttaccc caaagaagat aacacacttt tatatgaaat cacagcctat ctggaggcag
     3361 ctggcattca caacccactg aataagatat acataacaac aaagcgctta ccctattttc
     3421 caattgtaaa ctttctattt ttgatcgctc agttgccaaa acttcaatac aacaaaaatc
     3481 tgggaatggt ctgccgaaaa ccgaccgacc cggttgattg gccaccactt gtcctgggac
     3541 tgctcactct gctgaagcag ttccattccc ggtacaccga gcagttcctg gcgctgattg
     3601 gccagtttat ctgctccacg gtggagcagt gtacaagcca gaagatacct gaaattcctg
     3661 cagatgttgt gggtgccctt ctgttcctgg aggattatgt tcggtacaca aagctaccca
     3721 ggagggttgc tgaagcacat gtgcctaatt tcatttttga tgagttcaga acagtgctgt
     3781 aactgttttt cctacttctt caatggaagg attgtcctta gatcttccca ccatcacaaa
     3841 tgaatttgaa gatgaaaaga aactcagttg ctcatacaac tgcatttttt ctgtctatta
     3901 tgggaaacat cagacgttct gagtaagata tatctcatgg cattagttaa tataactgat
     3961 attgtttaaa tcatggtatt acatgcaatt tatatcagat aaaagcagaa cacatttttg
     4021 tactgcctct cttaaatgct gaatgtaact gttatgtata aatccattta gttttatgtt
     4081 ctaaagaact atttgtgcaa ctccagattt tcagtaaaat agtattacta gtacccaaa
//
