LOCUS       NM_016053                882 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens WASH complex subunit 3 (WASHC3), transcript variant 1,
            mRNA.
ACCESSION   NM_016053
VERSION     NM_016053.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 882)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   2  (bases 1 to 882)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   3  (bases 1 to 882)
  AUTHORS   Fragoza,R., Das,J., Wierbowski,S.D., Liang,J., Tran,T.N., Liang,S.,
            Beltran,J.F., Rivera-Erick,C.A., Ye,K., Wang,T.Y., Yao,L., Mort,M.,
            Stenson,P.D., Cooper,D.N., Wei,X., Keinan,A., Schimenti,J.C.,
            Clark,A.G. and Yu,H.
  TITLE     Extensive disruption of protein interactions by genetic variants
            across the allele frequency spectrum in human populations
  JOURNAL   Nat Commun 10 (1), 4141 (2019)
   PUBMED   31515488
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 882)
  AUTHORS   Chen,S., Fragoza,R., Klei,L., Liu,Y., Wang,J., Roeder,K., Devlin,B.
            and Yu,H.
  TITLE     An interactome perturbation framework prioritizes damaging missense
            mutations for developmental disorders
  JOURNAL   Nat Genet 50 (7), 1032-1040 (2018)
   PUBMED   29892012
REFERENCE   5  (bases 1 to 882)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Paulo,J.A., Cannon,J.R., Ting,L.,
            Baltier,K., Colby,G., Gebreab,F., Gygi,M.P., Parzen,H., Szpyt,J.,
            Tam,S., Zarraga,G., Pontano-Vaites,L., Swarup,S., White,A.E.,
            Schweppe,D.K., Rad,R., Erickson,B.K., Obar,R.A., Guruharsha,K.G.,
            Li,K., Artavanis-Tsakonas,S., Gygi,S.P. and Harper,J.W.
  TITLE     Architecture of the human interactome defines protein communities
            and disease networks
  JOURNAL   Nature 545 (7655), 505-509 (2017)
   PUBMED   28514442
REFERENCE   6  (bases 1 to 882)
  AUTHORS   Derivery,E., Sousa,C., Gautier,J.J., Lombard,B., Loew,D. and
            Gautreau,A.
  TITLE     The Arp2/3 activator WASH controls the fission of endosomes through
            a large multiprotein complex
  JOURNAL   Dev Cell 17 (5), 712-723 (2009)
   PUBMED   19922875
REFERENCE   7  (bases 1 to 882)
  AUTHORS   Gomez,T.S. and Billadeau,D.D.
  TITLE     A FAM21-containing WASH complex regulates retromer-dependent
            sorting
  JOURNAL   Dev Cell 17 (5), 699-711 (2009)
   PUBMED   19922874
REFERENCE   8  (bases 1 to 882)
  AUTHORS   Venkatesan,K., Rual,J.F., Vazquez,A., Stelzl,U., Lemmens,I.,
            Hirozane-Kishikawa,T., Hao,T., Zenkner,M., Xin,X., Goh,K.I.,
            Yildirim,M.A., Simonis,N., Heinzmann,K., Gebreab,F., Sahalie,J.M.,
            Cevik,S., Simon,C., de Smet,A.S., Dann,E., Smolyar,A.,
            Vinayagam,A., Yu,H., Szeto,D., Borick,H., Dricot,A., Klitgord,N.,
            Murray,R.R., Lin,C., Lalowski,M., Timm,J., Rau,K., Boone,C.,
            Braun,P., Cusick,M.E., Roth,F.P., Hill,D.E., Tavernier,J.,
            Wanker,E.E., Barabasi,A.L. and Vidal,M.
  TITLE     An empirical framework for binary interactome mapping
  JOURNAL   Nat Methods 6 (1), 83-90 (2009)
   PUBMED   19060904
REFERENCE   9  (bases 1 to 882)
  AUTHORS   Stelzl,U., Worm,U., Lalowski,M., Haenig,C., Brembeck,F.H.,
            Goehler,H., Stroedicke,M., Zenkner,M., Schoenherr,A., Koeppen,S.,
            Timm,J., Mintzlaff,S., Abraham,C., Bock,N., Kietzmann,S.,
            Goedde,A., Toksoz,E., Droege,A., Krobitsch,S., Korn,B.,
            Birchmeier,W., Lehrach,H. and Wanker,E.E.
  TITLE     A human protein-protein interaction network: a resource for
            annotating the proteome
  JOURNAL   Cell 122 (6), 957-968 (2005)
   PUBMED   16169070
REFERENCE   10 (bases 1 to 882)
  AUTHORS   Hu,R.M., Han,Z.G., Song,H.D., Peng,Y.D., Huang,Q.H., Ren,S.X.,
            Gu,Y.J., Huang,C.H., Li,Y.B., Jiang,C.L., Fu,G., Zhang,Q.H.,
            Gu,B.W., Dai,M., Mao,Y.F., Gao,G.F., Rong,R., Ye,M., Zhou,J.,
            Xu,S.H., Gu,J., Shi,J.X., Jin,W.R., Zhang,C.K., Wu,T.M.,
            Huang,G.Y., Chen,Z., Chen,M.D. and Chen,J.L.
  TITLE     Gene expression profiling in the human
            hypothalamus-pituitary-adrenal axis and full-length cDNA cloning
  JOURNAL   Proc Natl Acad Sci U S A 97 (17), 9543-9548 (2000)
   PUBMED   10931946
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AF151874.1 and AC079907.25.
            
            On May 17, 2019 this sequence version replaced NM_016053.3.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BG709411.1, AF155655.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000240079.11/ ENSP00000240079.6
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-780               AF151874.1         79-858
            781-882             AC079907.25        107844-107945
FEATURES             Location/Qualifiers
     source          1..882
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="12"
                     /map="12q23.2"
     gene            1..882
                     /gene="WASHC3"
                     /gene_synonym="CCDC53; CGI-116"
                     /note="WASH complex subunit 3"
                     /db_xref="GeneID:51019"
                     /db_xref="HGNC:HGNC:24256"
                     /db_xref="MIM:619925"
     exon            1..80
                     /gene="WASHC3"
                     /gene_synonym="CCDC53; CGI-116"
                     /inference="alignment:Splign:2.1.0"
     CDS             30..614
                     /gene="WASHC3"
                     /gene_synonym="CCDC53; CGI-116"
                     /note="isoform 1 is encoded by transcript variant 1; WASH
                     complex subunit CCDC53; coiled-coil domain-containing
                     protein 53; coiled-coil domain containing 53"
                     /codon_start=1
                     /product="WASH complex subunit 3 isoform 1"
                     /protein_id="NP_057137.1"
                     /db_xref="CCDS:CCDS44959.1"
                     /db_xref="GeneID:51019"
                     /db_xref="HGNC:HGNC:24256"
                     /db_xref="MIM:619925"
                     /translation="MDEDGLPLMGSGIDLTKVPAIQQKRTVAFLNQFVVHTVQFLNRF
                     STVCEEKLADLSLRIQQIETTLNILDAKLSSIPGLDDVTVEVSPLNVTSVTNGAHPEA
                     TSEQPQQNSTQDSGLQESEVSAENILTVAKDPRYARYLKMVQVGVPVMAIRNKMISEG
                     LDPDLLERPDAPVPDGESEKTVEESSDSESSFSD"
     exon            81..179
                     /gene="WASHC3"
                     /gene_synonym="CCDC53; CGI-116"
                     /inference="alignment:Splign:2.1.0"
     exon            180..245
                     /gene="WASHC3"
                     /gene_synonym="CCDC53; CGI-116"
                     /inference="alignment:Splign:2.1.0"
     exon            246..353
                     /gene="WASHC3"
                     /gene_synonym="CCDC53; CGI-116"
                     /inference="alignment:Splign:2.1.0"
     exon            354..464
                     /gene="WASHC3"
                     /gene_synonym="CCDC53; CGI-116"
                     /inference="alignment:Splign:2.1.0"
     exon            465..529
                     /gene="WASHC3"
                     /gene_synonym="CCDC53; CGI-116"
                     /inference="alignment:Splign:2.1.0"
     exon            530..882
                     /gene="WASHC3"
                     /gene_synonym="CCDC53; CGI-116"
                     /inference="alignment:Splign:2.1.0"
     regulatory      758..763
                     /regulatory_class="polyA_signal_sequence"
                     /gene="WASHC3"
                     /gene_synonym="CCDC53; CGI-116"
                     /note="hexamer: AATAAA"
     polyA_site      780
                     /gene="WASHC3"
                     /gene_synonym="CCDC53; CGI-116"
                     /note="major polyA site"
     regulatory      798..803
                     /regulatory_class="polyA_signal_sequence"
                     /gene="WASHC3"
                     /gene_synonym="CCDC53; CGI-116"
                     /note="hexamer: AATAAA"
     polyA_site      821
                     /gene="WASHC3"
                     /gene_synonym="CCDC53; CGI-116"
     regulatory      834..839
                     /regulatory_class="polyA_signal_sequence"
                     /gene="WASHC3"
                     /gene_synonym="CCDC53; CGI-116"
                     /note="hexamer: AATAAA"
     polyA_site      858
                     /gene="WASHC3"
                     /gene_synonym="CCDC53; CGI-116"
ORIGIN      
        1 gaactcgggg tccaccgccc gctgaggaga tggatgagga cgggcttcct ctcatggggt
       61 caggcataga cctgaccaag gtgccagcta ttcaacagaa aagaacggtg gcttttctaa
      121 accaatttgt ggtgcacact gtacagttcc tcaaccgctt ttctacagtt tgtgaggaga
      181 aactggcaga cctttcactt cgtatccaac aaattgaaac aactctcaat attttagatg
      241 caaagttgtc atctatccca ggcctagatg atgtcacagt tgaagtatct cctttaaatg
      301 tcaccagtgt cacaaatgga gcacatcctg aagccacttc agagcaacca cagcagaaca
      361 gtacacaaga ctctggacta caggaaagtg aagtatcagc agaaaatatc ttaactgtag
      421 ccaaggatcc aagatatgcc agatatctca aaatggttca agtgggtgta ccagtgatgg
      481 caataagaaa caaaatgata tcagaaggac tagacccaga tcttcttgag aggccagatg
      541 ctccagtgcc tgatggcgaa agtgagaaaa ctgtagaaga aagttcagat agcgaatctt
      601 cttttagtga ttaagcttaa ttttgataag aattacatat gcatgcatag gggtacattt
      661 acattctgta agagattgag cctgaactct cttagtcata aaaacatcaa atggccacat
      721 gtccactacc aagcttcttc tatgttaaaa aaataataat aaagcagttt taacctgcca
      781 gtatgtcttg ttgctaaaat aaagggcctc aaaatgaaaa ataggataac ctaaataaag
      841 tacaattagt gctacaaata ctaagataga atattttaga ga
//
