LOCUS       NM_001367414            4649 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens WASH complex subunit 2C (WASHC2C), transcript variant
            27, mRNA.
ACCESSION   NM_001367414
VERSION     NM_001367414.1
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4649)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   2  (bases 1 to 4649)
  AUTHORS   McNally,K.E., Faulkner,R., Steinberg,F., Gallon,M., Ghai,R.,
            Pim,D., Langton,P., Pearson,N., Danson,C.M., Nagele,H.,
            Morris,L.L., Singla,A., Overlee,B.L., Heesom,K.J., Sessions,R.,
            Banks,L., Collins,B.M., Berger,I., Billadeau,D.D., Burstein,E. and
            Cullen,P.J.
  TITLE     Retriever is a multiprotein complex for retromer-independent
            endosomal cargo recycling
  JOURNAL   Nat Cell Biol 19 (10), 1214-1225 (2017)
   PUBMED   28892079
REFERENCE   3  (bases 1 to 4649)
  AUTHORS   Follett,J., Bugarcic,A., Yang,Z., Ariotti,N., Norwood,S.J.,
            Collins,B.M., Parton,R.G. and Teasdale,R.D.
  TITLE     Parkinson Disease-linked Vps35 R524W Mutation Impairs the Endosomal
            Association of Retromer and Induces alpha-Synuclein Aggregation
  JOURNAL   J Biol Chem 291 (35), 18283-18298 (2016)
   PUBMED   27385586
REFERENCE   4  (bases 1 to 4649)
  AUTHORS   Deng,Z.H., Gomez,T.S., Osborne,D.G., Phillips-Krawczak,C.A.,
            Zhang,J.S. and Billadeau,D.D.
  TITLE     Nuclear FAM21 participates in NF-kappaB-dependent gene regulation
            in pancreatic cancer cells
  JOURNAL   J Cell Sci 128 (2), 373-384 (2015)
   PUBMED   25431135
  REMARK    GeneRIF: FAM21 not only functions as an integral component of the
            cytoplasmic WASH complex, but also modulates NF-kappaB gene
            transcription in the nucleus.
REFERENCE   5  (bases 1 to 4649)
  AUTHORS   Phillips-Krawczak,C.A., Singla,A., Starokadomskyy,P., Deng,Z.,
            Osborne,D.G., Li,H., Dick,C.J., Gomez,T.S., Koenecke,M.,
            Zhang,J.S., Dai,H., Sifuentes-Dominguez,L.F., Geng,L.N.,
            Kaufmann,S.H., Hein,M.Y., Wallis,M., McGaughran,J., Gecz,J.,
            Sluis,Bv., Billadeau,D.D. and Burstein,E.
  TITLE     COMMD1 is linked to the WASH complex and regulates endosomal
            trafficking of the copper transporter ATP7A
  JOURNAL   Mol Biol Cell 26 (1), 91-103 (2015)
   PUBMED   25355947
REFERENCE   6  (bases 1 to 4649)
  AUTHORS   Hernandez-Valladares,M., Kim,T., Kannan,B., Tung,A., Aguda,A.H.,
            Larsson,M., Cooper,J.A. and Robinson,R.C.
  TITLE     Structural characterization of a capping protein interaction motif
            defines a family of actin filament regulators
  JOURNAL   Nat Struct Mol Biol 17 (4), 497-503 (2010)
   PUBMED   20357771
REFERENCE   7  (bases 1 to 4649)
  AUTHORS   Kamatani,Y., Matsuda,K., Okada,Y., Kubo,M., Hosono,N., Daigo,Y.,
            Nakamura,Y. and Kamatani,N.
  TITLE     Genome-wide association study of hematological and biochemical
            traits in a Japanese population
  JOURNAL   Nat Genet 42 (3), 210-215 (2010)
   PUBMED   20139978
REFERENCE   8  (bases 1 to 4649)
  AUTHORS   Derivery,E., Sousa,C., Gautier,J.J., Lombard,B., Loew,D. and
            Gautreau,A.
  TITLE     The Arp2/3 activator WASH controls the fission of endosomes through
            a large multiprotein complex
  JOURNAL   Dev Cell 17 (5), 712-723 (2009)
   PUBMED   19922875
REFERENCE   9  (bases 1 to 4649)
  AUTHORS   Gomez,T.S. and Billadeau,D.D.
  TITLE     A FAM21-containing WASH complex regulates retromer-dependent
            sorting
  JOURNAL   Dev Cell 17 (5), 699-711 (2009)
   PUBMED   19922874
  REMARK    GeneRIF: WASH exists in a multiprotein complex containing FAM21,
            which links WASH to endosomes and is required for WASH-dependent
            retromer-mediated sorting.
REFERENCE   10 (bases 1 to 4649)
  AUTHORS   Huang,C.Y., Lu,T.Y., Bair,C.H., Chang,Y.S., Jwo,J.K. and Chang,W.
  TITLE     A novel cellular protein, VPEF, facilitates vaccinia virus
            penetration into HeLa cells through fluid phase endocytosis
  JOURNAL   J Virol 82 (16), 7988-7999 (2008)
   PUBMED   18550675
  REMARK    GeneRIF: a cellular factor, VPEF, is exploited by vaccinia virus
            for cell entry through fluid phase endocytosis when vaccinia virus
            enters HeLa cells
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL645998.10, AL731535.6 and AC012044.15.
            
            Transcript Variant: This variant (27), as well as variants 24, 25,
            and 26, encodes isoform 24.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803617.91148.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2142586, SAMEA2148874
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-55                AL645998.10        8822-8876
            56-178              AL645998.10        8973-9095
            179-299             AL731535.6         2021-2141
            300-362             AL731535.6         11098-11160
            363-536             AL731535.6         13188-13361
            537-650             AL731535.6         14938-15051
            651-744             AL731535.6         16505-16598
            745-806             AL731535.6         17936-17997
            807-854             AL731535.6         19715-19762
            855-965             AL731535.6         23211-23321
            966-1053            AL731535.6         23866-23953
            1054-1125           AL731535.6         24597-24668
            1126-1244           AL731535.6         25703-25821
            1245-1302           AL731535.6         26295-26352
            1303-1362           AL731535.6         27601-27660
            1363-1542           AL731535.6         28051-28230
            1543-1670           AL731535.6         30127-30254
            1671-1757           AL731535.6         32430-32516
            1758-1859           AC012044.15        168028-168129       c
            1860-1991           AC012044.15        165707-165838       c
            1992-2161           AC012044.15        161899-162068       c
            2162-2264           AC012044.15        158159-158261       c
            2265-2417           AC012044.15        154092-154244       c
            2418-2600           AC012044.15        152382-152564       c
            2601-2729           AC012044.15        146824-146952       c
            2730-2810           AC012044.15        146616-146696       c
            2811-2933           AC012044.15        145886-146008       c
            2934-3146           AC012044.15        144270-144482       c
            3147-3767           AC012044.15        142026-142646       c
            3768-3945           AC012044.15        140984-141161       c
            3946-4649           AC012044.15        138553-139256       c
FEATURES             Location/Qualifiers
     source          1..4649
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="10"
                     /map="10q11.22"
     gene            1..4649
                     /gene="WASHC2C"
                     /gene_synonym="FAM21A; FAM21C; VPEF"
                     /note="WASH complex subunit 2C"
                     /db_xref="GeneID:253725"
                     /db_xref="HGNC:HGNC:23414"
                     /db_xref="MIM:613631"
     exon            1..55
                     /gene="WASHC2C"
                     /gene_synonym="FAM21A; FAM21C; VPEF"
                     /inference="alignment:Splign:2.1.0"
     exon            56..178
                     /gene="WASHC2C"
                     /gene_synonym="FAM21A; FAM21C; VPEF"
                     /inference="alignment:Splign:2.1.0"
     exon            179..299
                     /gene="WASHC2C"
                     /gene_synonym="FAM21A; FAM21C; VPEF"
                     /inference="alignment:Splign:2.1.0"
     exon            300..362
                     /gene="WASHC2C"
                     /gene_synonym="FAM21A; FAM21C; VPEF"
                     /inference="alignment:Splign:2.1.0"
     exon            363..536
                     /gene="WASHC2C"
                     /gene_synonym="FAM21A; FAM21C; VPEF"
                     /inference="alignment:Splign:2.1.0"
     exon            537..650
                     /gene="WASHC2C"
                     /gene_synonym="FAM21A; FAM21C; VPEF"
                     /inference="alignment:Splign:2.1.0"
     exon            651..744
                     /gene="WASHC2C"
                     /gene_synonym="FAM21A; FAM21C; VPEF"
                     /inference="alignment:Splign:2.1.0"
     CDS             702..4085
                     /gene="WASHC2C"
                     /gene_synonym="FAM21A; FAM21C; VPEF"
                     /note="isoform 24 is encoded by transcript variant 27;
                     WASH complex subunit FAM21C; vaccinia virus penetration
                     factor; family with sequence similarity 21 member C"
                     /codon_start=1
                     /product="WASH complex subunit 2C isoform 24"
                     /protein_id="NP_001354343.1"
                     /db_xref="GeneID:253725"
                     /db_xref="HGNC:HGNC:23414"
                     /db_xref="MIM:613631"
                     /translation="MEQEDVGLGELSSEEGSVGSDRGSIVDTEEEKEEEESDEDFAHH
                     SDNEQNQHTTQMSDEEEDDDGCDLFADSEKEEEDIEDIEENTRPKRSRPTSFADELAA
                     RIKGDAMGRVDEEPTTLPSGEAKPRKTLKEKKERRTPSDDEEDNLFAPPKLTDEDFSP
                     FGSGGGLFSGGKGLFDDEDEESDLFTEASQDRQAGASVKEESSSSKPGKKIPAGAVSV
                     FLGDTDVFGAASVPSLKEPQKPEQPTPRKSPYGPPPTGLFDDDDGDDDDDFFSAPHSK
                     PSKTRKVQSTADIFGDEEGDLFKEKAVASPEATVSQTDENKARAEKKVTLSYSKNLKP
                     SSETKTQKGLFSDEEDSEDLFSSQSASNLKGASLLPGKLPTSVSLFDDEDEEDNLFGG
                     TAAKKQTLSLQAQREEKAKASELSKKKASALLFSSDEEDQWNIPASQTHLASDSRSKG
                     EPRDSGTLQSQEAKAVKKTSLFEEDKEDDLFAIAKDSQKKTQRVSLLFEDDVDSGGSL
                     FGSPPTSVPPATKKKETVSEAPPLLFSDEEEKEAQLGVKSVDKKVESAKESLKFGRTD
                     VAESEKEGLLTRSAQETVKHSDLFSSSSPWDKGTKPRTKTVLSLFDEEEDKMEDQNII
                     QAPQKEVGKGCDPDAHPKSTGVFQDEELLFSHKLQKDNDPDVDLFAGTKKTKLLEPSV
                     GSLFGDDEDDDLFSSAKSQPLVQEKKRVVKKDHSVNSFKNQKHPESIQGSKEKGIWKP
                     ETPQANLAINPAALLPTAASQISEVKPVLPELAFPSSEHRRSHGLESVPVLPGSGEAG
                     VSFDLPAQADTLHSANKSRVKMRGKRRPQTRAARRLAAQESSEAEDMSVPRGPIAQWA
                     DGAISPNGHRPQLRAASGEDSTEEALAAAAAPWEGGPVPGVDTSPFAKSLGHSRGEAD
                     LFDSGDIFSTGTGSQSVERTKPKAKIAENPANPPVGGKAKSPMFPALGEASSDDDLFQ
                     SAKPKPAKKTNPFPLLEDEDDLFTDQKVKKNETKSSSQQDVILTTQDIFEDDIFATEA
                     IKPSQKTREKEKTLESNLFDDNIDIFADLTVKPKEKSKKKVEAKSIFDDDMDDIFSTG
                     IQAKTTKPKSRSAQAAPEPRFEHKVSNIFDDPLNAFGGQ"
     exon            745..806
                     /gene="WASHC2C"
                     /gene_synonym="FAM21A; FAM21C; VPEF"
                     /inference="alignment:Splign:2.1.0"
     exon            807..854
                     /gene="WASHC2C"
                     /gene_synonym="FAM21A; FAM21C; VPEF"
                     /inference="alignment:Splign:2.1.0"
     exon            855..965
                     /gene="WASHC2C"
                     /gene_synonym="FAM21A; FAM21C; VPEF"
                     /inference="alignment:Splign:2.1.0"
     exon            966..1053
                     /gene="WASHC2C"
                     /gene_synonym="FAM21A; FAM21C; VPEF"
                     /inference="alignment:Splign:2.1.0"
     exon            1054..1125
                     /gene="WASHC2C"
                     /gene_synonym="FAM21A; FAM21C; VPEF"
                     /inference="alignment:Splign:2.1.0"
     exon            1126..1244
                     /gene="WASHC2C"
                     /gene_synonym="FAM21A; FAM21C; VPEF"
                     /inference="alignment:Splign:2.1.0"
     exon            1245..1302
                     /gene="WASHC2C"
                     /gene_synonym="FAM21A; FAM21C; VPEF"
                     /inference="alignment:Splign:2.1.0"
     exon            1303..1362
                     /gene="WASHC2C"
                     /gene_synonym="FAM21A; FAM21C; VPEF"
                     /inference="alignment:Splign:2.1.0"
     exon            1363..1542
                     /gene="WASHC2C"
                     /gene_synonym="FAM21A; FAM21C; VPEF"
                     /inference="alignment:Splign:2.1.0"
     exon            1543..1670
                     /gene="WASHC2C"
                     /gene_synonym="FAM21A; FAM21C; VPEF"
                     /inference="alignment:Splign:2.1.0"
     exon            1671..1757
                     /gene="WASHC2C"
                     /gene_synonym="FAM21A; FAM21C; VPEF"
                     /inference="alignment:Splign:2.1.0"
     exon            1758..1859
                     /gene="WASHC2C"
                     /gene_synonym="FAM21A; FAM21C; VPEF"
                     /inference="alignment:Splign:2.1.0"
     exon            1860..1991
                     /gene="WASHC2C"
                     /gene_synonym="FAM21A; FAM21C; VPEF"
                     /inference="alignment:Splign:2.1.0"
     exon            1992..2161
                     /gene="WASHC2C"
                     /gene_synonym="FAM21A; FAM21C; VPEF"
                     /inference="alignment:Splign:2.1.0"
     exon            2162..2264
                     /gene="WASHC2C"
                     /gene_synonym="FAM21A; FAM21C; VPEF"
                     /inference="alignment:Splign:2.1.0"
     exon            2265..2417
                     /gene="WASHC2C"
                     /gene_synonym="FAM21A; FAM21C; VPEF"
                     /inference="alignment:Splign:2.1.0"
     exon            2418..2600
                     /gene="WASHC2C"
                     /gene_synonym="FAM21A; FAM21C; VPEF"
                     /inference="alignment:Splign:2.1.0"
     exon            2601..2729
                     /gene="WASHC2C"
                     /gene_synonym="FAM21A; FAM21C; VPEF"
                     /inference="alignment:Splign:2.1.0"
     exon            2730..2810
                     /gene="WASHC2C"
                     /gene_synonym="FAM21A; FAM21C; VPEF"
                     /inference="alignment:Splign:2.1.0"
     exon            2811..2933
                     /gene="WASHC2C"
                     /gene_synonym="FAM21A; FAM21C; VPEF"
                     /inference="alignment:Splign:2.1.0"
     exon            2934..3146
                     /gene="WASHC2C"
                     /gene_synonym="FAM21A; FAM21C; VPEF"
                     /inference="alignment:Splign:2.1.0"
     exon            3147..3767
                     /gene="WASHC2C"
                     /gene_synonym="FAM21A; FAM21C; VPEF"
                     /inference="alignment:Splign:2.1.0"
     exon            3768..3945
                     /gene="WASHC2C"
                     /gene_synonym="FAM21A; FAM21C; VPEF"
                     /inference="alignment:Splign:2.1.0"
     exon            3946..4649
                     /gene="WASHC2C"
                     /gene_synonym="FAM21A; FAM21C; VPEF"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4269..4274
                     /regulatory_class="polyA_signal_sequence"
                     /gene="WASHC2C"
                     /gene_synonym="FAM21A; FAM21C; VPEF"
                     /note="hexamer: AATAAA"
     polyA_site      4286
                     /gene="WASHC2C"
                     /gene_synonym="FAM21A; FAM21C; VPEF"
     regulatory      4622..4627
                     /regulatory_class="polyA_signal_sequence"
                     /gene="WASHC2C"
                     /gene_synonym="FAM21A; FAM21C; VPEF"
                     /note="hexamer: AATAAA"
     polyA_site      4649
                     /gene="WASHC2C"
                     /gene_synonym="FAM21A; FAM21C; VPEF"
                     /note="major polyA site"
ORIGIN      
        1 ggtcctcggc ctgtgctggc agcctcggag cccaccgagc cgggcggctg ggatgatgaa
       61 ccggacgacc cccgaccagg agctggtgcc ggcgtcggag cccgtgtggg agcggccgtg
      121 gtcggtggag gagatccgca ggagcagcca gagctggtcg ctggcggccg acgcgggcga
      181 cccatgaaat caagaaacaa gtggacggac taatccggga aaccaaagcc acagattgtc
      241 gcctgcataa tgtcttcaat gacttcctta tgctctctaa tacccagttc atagagaatc
      301 gtgtatatga tgaagaagtg gaggagccag tactcaaggc tgaggcagaa aaaacagagc
      361 aggagaagac acgagagcag aaagaagtag atctcattcc taaagtccag gaggctgtga
      421 actatggctt acaagtattg gacagtgcct ttgagcaact tgatatcaaa gcaggaaact
      481 cagactccga ggaagatgat gctaatgggc gggtggaact gatccttgaa ccaaagagtt
      541 tcactcttgt tgcccaagct ggagtggcgc aatctcggct cactgcagct tctgtctcct
      601 gggttcaagc gattctcctg cctcagcctt ccaagtagct gggattacag gatctataca
      661 ttgatcgtcc tttaccatat ctcattgggt caaagctgtt catggaacaa gaagatgtag
      721 gtcttggaga gctgtccagt gaagaaggct ctgtaggcag tgatcgtggc agtattgtgg
      781 acactgagga agagaaagaa gaggaggagt cagatgaaga ttttgcccat cacagtgaca
      841 atgaacaaaa ccagcacacc acacaaatga gtgatgagga agaggatgat gatggctgtg
      901 acctttttgc tgactctgag aaggaggagg aagatattga ggacattgaa gaaaatacta
      961 gacctaaaag aagcagacct acatcgtttg cagatgagct ggctgcccgc atcaaggggg
     1021 atgccatggg tcgagtggac gaggagccga caaccttacc ctcaggagaa gcaaaacctc
     1081 ggaagacact caaagagaag aaggaaagga gaactccttc agacgatgaa gaggataact
     1141 tattcgcacc ccccaagctg accgacgagg acttctcgcc atttggctct ggaggtggcc
     1201 tgttcagtgg cggcaagggg ctatttgatg atgaggacga ggagagtgac ctcttcacgg
     1261 aagcctccca ggatcggcaa gctggagcct ctgttaagga ggagtcttca tcatccaaac
     1321 ctggaaagaa aatcccagca ggagctgttt ctgtattttt aggagacacg gatgtgtttg
     1381 gtgctgcctc cgttccatca ctgaaggagc cacagaagcc tgagcagccc actccaagga
     1441 aaagccccta tggtccccct cccactggcc tctttgatga tgatgatggt gatgatgatg
     1501 acgacttttt ctcggcaccc cacagcaaac cttctaaaac acgcaaagtc caatccactg
     1561 ccgatatctt tggtgacgaa gaaggagatc tgttcaaaga aaaagccgta gcatcgccag
     1621 aagccactgt gagtcagaca gatgaaaata aagcaagagc agaaaaaaag gttaccttat
     1681 cttacagcaa aaatctcaag ccctcatcag aaacaaagac tcaaaaaggc ttattttcag
     1741 atgaggagga ctctgaggat ttgttttctt ctcaaagtgc gagtaactta aaaggtgcat
     1801 ctctgctgcc tggcaagctc cccacgtcgg tttccctgtt tgatgatgaa gatgaagagg
     1861 ataatctttt tgggggtaca gctgctaaga agcagacatt gtctctacaa gctcagagag
     1921 aagagaaagc aaaagcctcc gagctctcca aaaagaaagc atctgccctg ttgttcagca
     1981 gtgatgagga ggaccagtgg aatattcctg cttcacagac ccacttagca tctgacagca
     2041 ggtctaaagg agaacccagg gattctggga ccctccagag ccaggaggcc aaggctgtga
     2101 aaaagaccag tctctttgag gaagacaaag aagatgatct ttttgccatt gccaaggaca
     2161 gccaaaagaa gacccagaga gtgtcactcc tctttgaaga cgatgttgat agcggaggct
     2221 ctctgtttgg ctctcctccc acatctgttc ctcctgcaac aaagaaaaaa gagactgtct
     2281 ctgaggcacc acctttgctg ttcagcgatg aagaagagaa ggaggcacaa cttggagtga
     2341 agtctgtgga taagaaggtt gagagtgcca aggagtcatt aaaatttggg agaactgatg
     2401 tggctgagtc agaaaaggaa ggacttttga ctagatctgc tcaggaaaca gtcaagcatt
     2461 ctgatttatt ttcttcatca tccccatggg acaaaggaac caagcctaga accaaaactg
     2521 ttcttagctt gtttgatgag gaagaggata aaatggaaga tcaaaacatt atccaggctc
     2581 cacagaaaga agtaggaaag ggctgcgatc ctgatgccca ccccaagagc acaggtgtct
     2641 tccaggatga agagctgctt ttcagccaca agctccaaaa ggacaatgac ccagatgttg
     2701 acctttttgc tggcaccaaa aaaaccaagc tgttagagcc aagtgttggg agcctgtttg
     2761 gggatgatga agatgatgat cttttcagct ctgccaagtc ccagcctttg gtacaagaga
     2821 aaaagagagt agtgaaaaaa gaccactctg ttaactcttt caaaaaccag aaacatcctg
     2881 aatccattca aggtagtaaa gaaaaaggca tatggaagcc ggaaacacct caggcaaatt
     2941 tagcgatcaa cccagcggcc ttgctgccca cagcggcttc ccagatctct gaagtaaagc
     3001 ctgttttgcc agaattggct tttccttcat ctgaacacag aaggagccac ggtctggaaa
     3061 gtgtgcctgt ccttcccggg agtggggagg ccggtgtgag ttttgatctt ccagctcagg
     3121 cagacacctt acacagtgca aacaagagcc gtgtcaagat gagagggaag cgtagaccgc
     3181 agacccgtgc agctaggcgg ctggctgctc aggagtccag cgaggctgag gacatgagcg
     3241 tccccagagg acccattgca cagtgggctg atggcgccat ttccccaaat ggccatcggc
     3301 cacagctcag agcagccagt ggagaagaca gcactgagga ggccctggca gctgccgctg
     3361 caccttggga aggtggtcct gtgcctggag tggacacaag cccctttgca aagtctctgg
     3421 gtcattccag aggggaggct gacctttttg attctgggga cattttttcc acgggcactg
     3481 gatctcagtc cgtggagaga acaaaaccca aggcaaagat agcagagaat cctgccaacc
     3541 caccagtggg tggtaaagca aagagcccca tgtttcctgc tctaggcgag gccagcagtg
     3601 atgatgatct ctttcagtct gctaaaccaa aaccagcaaa gaaaacaaat ccctttcctc
     3661 tcctggaaga tgaggatgac ctctttacag atcagaaagt caagaagaat gagacaaaat
     3721 ccagtagtca gcaggatgtc atattaacaa cacaagatat ttttgaggat gatatatttg
     3781 ctacggaagc aattaaaccc tctcagaaaa ccagagagaa ggagaaaaca ttggaatcta
     3841 atttatttga tgataacatt gatatctttg ctgacttaac tgtaaaacca aaagaaaagt
     3901 ccaaaaagaa agtggaagcc aagtctatat ttgatgatga tatggatgac atcttctcca
     3961 ctggtatcca ggctaagaca accaaaccaa aaagccgatc tgcacaggcc gcacctgaac
     4021 caagatttga acacaaggtg tccaacatct ttgatgatcc cctgaatgcc tttggaggcc
     4081 agtagagcac acagggtatc cacatgttac cctgcagcta cattgttgag ttagtgatga
     4141 tattgtatat gctcatggtc ttaactggat tacaaaaagc aaatactaga acagctagct
     4201 catcttttac ccaatgtact tagtattttt ctgcactggt ttaatcatgc ttaatactac
     4261 aaaacaaaaa taaatatttc acagtggttg gtttgttttg tttttaaacc acagtttgat
     4321 ttagttagcc ttgctggggc cataatatgc ttcagggtgt gtaaaagaag aaatctcttt
     4381 gtggctttca tgggcaggga atctcagaga tagcaaatgc cacctgacca gaagtctttg
     4441 ttatatggat gggaacccta acttagggct tgggcagggg aaagagaaag aagatgagag
     4501 attatacttc atgagtctta gcaatatggg agcaggtttt cactgaattc tgagggtgcc
     4561 tctgcatgtc ctccaaggca aagtttggca aactgtggcc cccccactgt catattttgt
     4621 taataaaatt ttattggaac acaatcaca
//
