LOCUS       NM_001330074            4642 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens WASH complex subunit 2C (WASHC2C), transcript variant
            4, mRNA.
ACCESSION   NM_001330074 XM_011539566
VERSION     NM_001330074.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4642)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   2  (bases 1 to 4642)
  AUTHORS   McNally,K.E., Faulkner,R., Steinberg,F., Gallon,M., Ghai,R.,
            Pim,D., Langton,P., Pearson,N., Danson,C.M., Nagele,H.,
            Morris,L.L., Singla,A., Overlee,B.L., Heesom,K.J., Sessions,R.,
            Banks,L., Collins,B.M., Berger,I., Billadeau,D.D., Burstein,E. and
            Cullen,P.J.
  TITLE     Retriever is a multiprotein complex for retromer-independent
            endosomal cargo recycling
  JOURNAL   Nat Cell Biol 19 (10), 1214-1225 (2017)
   PUBMED   28892079
REFERENCE   3  (bases 1 to 4642)
  AUTHORS   Follett,J., Bugarcic,A., Yang,Z., Ariotti,N., Norwood,S.J.,
            Collins,B.M., Parton,R.G. and Teasdale,R.D.
  TITLE     Parkinson Disease-linked Vps35 R524W Mutation Impairs the Endosomal
            Association of Retromer and Induces alpha-Synuclein Aggregation
  JOURNAL   J Biol Chem 291 (35), 18283-18298 (2016)
   PUBMED   27385586
REFERENCE   4  (bases 1 to 4642)
  AUTHORS   Deng,Z.H., Gomez,T.S., Osborne,D.G., Phillips-Krawczak,C.A.,
            Zhang,J.S. and Billadeau,D.D.
  TITLE     Nuclear FAM21 participates in NF-kappaB-dependent gene regulation
            in pancreatic cancer cells
  JOURNAL   J Cell Sci 128 (2), 373-384 (2015)
   PUBMED   25431135
  REMARK    GeneRIF: FAM21 not only functions as an integral component of the
            cytoplasmic WASH complex, but also modulates NF-kappaB gene
            transcription in the nucleus.
REFERENCE   5  (bases 1 to 4642)
  AUTHORS   Phillips-Krawczak,C.A., Singla,A., Starokadomskyy,P., Deng,Z.,
            Osborne,D.G., Li,H., Dick,C.J., Gomez,T.S., Koenecke,M.,
            Zhang,J.S., Dai,H., Sifuentes-Dominguez,L.F., Geng,L.N.,
            Kaufmann,S.H., Hein,M.Y., Wallis,M., McGaughran,J., Gecz,J.,
            Sluis,Bv., Billadeau,D.D. and Burstein,E.
  TITLE     COMMD1 is linked to the WASH complex and regulates endosomal
            trafficking of the copper transporter ATP7A
  JOURNAL   Mol Biol Cell 26 (1), 91-103 (2015)
   PUBMED   25355947
REFERENCE   6  (bases 1 to 4642)
  AUTHORS   Hernandez-Valladares,M., Kim,T., Kannan,B., Tung,A., Aguda,A.H.,
            Larsson,M., Cooper,J.A. and Robinson,R.C.
  TITLE     Structural characterization of a capping protein interaction motif
            defines a family of actin filament regulators
  JOURNAL   Nat Struct Mol Biol 17 (4), 497-503 (2010)
   PUBMED   20357771
REFERENCE   7  (bases 1 to 4642)
  AUTHORS   Kamatani,Y., Matsuda,K., Okada,Y., Kubo,M., Hosono,N., Daigo,Y.,
            Nakamura,Y. and Kamatani,N.
  TITLE     Genome-wide association study of hematological and biochemical
            traits in a Japanese population
  JOURNAL   Nat Genet 42 (3), 210-215 (2010)
   PUBMED   20139978
REFERENCE   8  (bases 1 to 4642)
  AUTHORS   Derivery,E., Sousa,C., Gautier,J.J., Lombard,B., Loew,D. and
            Gautreau,A.
  TITLE     The Arp2/3 activator WASH controls the fission of endosomes through
            a large multiprotein complex
  JOURNAL   Dev Cell 17 (5), 712-723 (2009)
   PUBMED   19922875
REFERENCE   9  (bases 1 to 4642)
  AUTHORS   Gomez,T.S. and Billadeau,D.D.
  TITLE     A FAM21-containing WASH complex regulates retromer-dependent
            sorting
  JOURNAL   Dev Cell 17 (5), 699-711 (2009)
   PUBMED   19922874
  REMARK    GeneRIF: WASH exists in a multiprotein complex containing FAM21,
            which links WASH to endosomes and is required for WASH-dependent
            retromer-mediated sorting.
REFERENCE   10 (bases 1 to 4642)
  AUTHORS   Huang,C.Y., Lu,T.Y., Bair,C.H., Chang,Y.S., Jwo,J.K. and Chang,W.
  TITLE     A novel cellular protein, VPEF, facilitates vaccinia virus
            penetration into HeLa cells through fluid phase endocytosis
  JOURNAL   J Virol 82 (16), 7988-7999 (2008)
   PUBMED   18550675
  REMARK    GeneRIF: a cellular factor, VPEF, is exploited by vaccinia virus
            for cell entry through fluid phase endocytosis when vaccinia virus
            enters HeLa cells
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL645998.10, AL731535.6, AC012044.15 and AI023041.1.
            
            On Jun 2, 2019 this sequence version replaced NM_001330074.1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803614.138642.1,
                                           SRR1803613.163436.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000623400.4/ ENSP00000485513.1
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-55                AL645998.10        8822-8876
            56-178              AL645998.10        8973-9095
            179-202             AL645998.10        10418-10441
            203-343             AL731535.6         2001-2141
            344-406             AL731535.6         11098-11160
            407-580             AL731535.6         13188-13361
            581-674             AL731535.6         16505-16598
            675-736             AL731535.6         17936-17997
            737-784             AL731535.6         19715-19762
            785-895             AL731535.6         23211-23321
            896-983             AL731535.6         23866-23953
            984-1055            AL731535.6         24597-24668
            1056-1174           AL731535.6         25703-25821
            1175-1232           AL731535.6         26295-26352
            1233-1292           AL731535.6         27601-27660
            1293-1472           AL731535.6         28051-28230
            1473-1600           AL731535.6         30127-30254
            1601-1687           AL731535.6         32430-32516
            1688-1789           AC012044.15        168028-168129       c
            1790-1921           AC012044.15        165707-165838       c
            1922-2091           AC012044.15        161899-162068       c
            2092-2194           AC012044.15        158159-158261       c
            2195-2347           AC012044.15        154092-154244       c
            2348-2530           AC012044.15        152382-152564       c
            2531-2659           AC012044.15        146824-146952       c
            2660-2740           AC012044.15        146616-146696       c
            2741-2863           AC012044.15        145886-146008       c
            2864-2926           AC012044.15        144843-144905       c
            2927-3139           AC012044.15        144270-144482       c
            3140-3760           AC012044.15        142026-142646       c
            3761-3938           AC012044.15        140984-141161       c
            3939-4629           AC012044.15        138566-139256       c
            4630-4642           AI023041.1         9-21                c
FEATURES             Location/Qualifiers
     source          1..4642
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="10"
                     /map="10q11.22"
     gene            1..4642
                     /gene="WASHC2C"
                     /gene_synonym="FAM21A; FAM21C; VPEF"
                     /note="WASH complex subunit 2C"
                     /db_xref="GeneID:253725"
                     /db_xref="HGNC:HGNC:23414"
                     /db_xref="MIM:613631"
     exon            1..55
                     /gene="WASHC2C"
                     /gene_synonym="FAM21A; FAM21C; VPEF"
                     /inference="alignment:Splign:2.1.0"
     CDS             53..4078
                     /gene="WASHC2C"
                     /gene_synonym="FAM21A; FAM21C; VPEF"
                     /note="isoform 4 is encoded by transcript variant 4; WASH
                     complex subunit FAM21C; vaccinia virus penetration factor;
                     family with sequence similarity 21 member C"
                     /codon_start=1
                     /product="WASH complex subunit 2C isoform 4"
                     /protein_id="NP_001317003.1"
                     /db_xref="CCDS:CCDS81453.1"
                     /db_xref="GeneID:253725"
                     /db_xref="HGNC:HGNC:23414"
                     /db_xref="MIM:613631"
                     /translation="MMNRTTPDQELVPASEPVWERPWSVEEIRRSSQSWSLAADAGLL
                     QFLQEFSQQTISRTHEIKKQVDGLIRETKATDCRLHNVFNDFLMLSNTQFIENRVYDE
                     EVEEPVLKAEAEKTEQEKTREQKEVDLIPKVQEAVNYGLQVLDSAFEQLDIKAGNSDS
                     EEDDANGRVELILEPKDLYIDRPLPYLIGSKLFMEQEDVGLGELSSEEGSVGSDRGSI
                     VDTEEEKEEEESDEDFAHHSDNEQNQHTTQMSDEEEDDDGCDLFADSEKEEEDIEDIE
                     ENTRPKRSRPTSFADELAARIKGDAMGRVDEEPTTLPSGEAKPRKTLKEKKERRTPSD
                     DEEDNLFAPPKLTDEDFSPFGSGGGLFSGGKGLFDDEDEESDLFTEASQDRQAGASVK
                     EESSSSKPGKKIPAGAVSVFLGDTDVFGAASVPSLKEPQKPEQPTPRKSPYGPPPTGL
                     FDDDDGDDDDDFFSAPHSKPSKTRKVQSTADIFGDEEGDLFKEKAVASPEATVSQTDE
                     NKARAEKKVTLSYSKNLKPSSETKTQKGLFSDEEDSEDLFSSQSASNLKGASLLPGKL
                     PTSVSLFDDEDEEDNLFGGTAAKKQTLSLQAQREEKAKASELSKKKASALLFSSDEED
                     QWNIPASQTHLASDSRSKGEPRDSGTLQSQEAKAVKKTSLFEEDKEDDLFAIAKDSQK
                     KTQRVSLLFEDDVDSGGSLFGSPPTSVPPATKKKETVSEAPPLLFSDEEEKEAQLGVK
                     SVDKKVESAKESLKFGRTDVAESEKEGLLTRSAQETVKHSDLFSSSSPWDKGTKPRTK
                     TVLSLFDEEEDKMEDQNIIQAPQKEVGKGCDPDAHPKSTGVFQDEELLFSHKLQKDND
                     PDVDLFAGTKKTKLLEPSVGSLFGDDEDDDLFSSAKSQPLVQEKKRVVKKDHSVNSFK
                     NQKHPESIQGSKEKGIWKPETPQDSSGLAPFKTKEPSTRIGKIQANLAINPAALLPTA
                     ASQISEVKPVLPELAFPSSEHRRSHGLESVPVLPGSGEAGVSFDLPAQADTLHSANKS
                     RVKMRGKRRPQTRAARRLAAQESSEAEDMSVPRGPIAQWADGAISPNGHRPQLRAASG
                     EDSTEEALAAAAAPWEGGPVPGVDTSPFAKSLGHSRGEADLFDSGDIFSTGTGSQSVE
                     RTKPKAKIAENPANPPVGGKAKSPMFPALGEASSDDDLFQSAKPKPAKKTNPFPLLED
                     EDDLFTDQKVKKNETKSSSQQDVILTTQDIFEDDIFATEAIKPSQKTREKEKTLESNL
                     FDDNIDIFADLTVKPKEKSKKKVEAKSIFDDDMDDIFSTGIQAKTTKPKSRSAQAAPE
                     PRFEHKVSNIFDDPLNAFGGQ"
     exon            56..178
                     /gene="WASHC2C"
                     /gene_synonym="FAM21A; FAM21C; VPEF"
                     /inference="alignment:Splign:2.1.0"
     exon            179..343
                     /gene="WASHC2C"
                     /gene_synonym="FAM21A; FAM21C; VPEF"
                     /inference="alignment:Splign:2.1.0"
     exon            344..406
                     /gene="WASHC2C"
                     /gene_synonym="FAM21A; FAM21C; VPEF"
                     /inference="alignment:Splign:2.1.0"
     exon            407..580
                     /gene="WASHC2C"
                     /gene_synonym="FAM21A; FAM21C; VPEF"
                     /inference="alignment:Splign:2.1.0"
     exon            581..674
                     /gene="WASHC2C"
                     /gene_synonym="FAM21A; FAM21C; VPEF"
                     /inference="alignment:Splign:2.1.0"
     exon            675..736
                     /gene="WASHC2C"
                     /gene_synonym="FAM21A; FAM21C; VPEF"
                     /inference="alignment:Splign:2.1.0"
     exon            737..784
                     /gene="WASHC2C"
                     /gene_synonym="FAM21A; FAM21C; VPEF"
                     /inference="alignment:Splign:2.1.0"
     exon            785..895
                     /gene="WASHC2C"
                     /gene_synonym="FAM21A; FAM21C; VPEF"
                     /inference="alignment:Splign:2.1.0"
     exon            896..983
                     /gene="WASHC2C"
                     /gene_synonym="FAM21A; FAM21C; VPEF"
                     /inference="alignment:Splign:2.1.0"
     exon            984..1055
                     /gene="WASHC2C"
                     /gene_synonym="FAM21A; FAM21C; VPEF"
                     /inference="alignment:Splign:2.1.0"
     exon            1056..1174
                     /gene="WASHC2C"
                     /gene_synonym="FAM21A; FAM21C; VPEF"
                     /inference="alignment:Splign:2.1.0"
     exon            1175..1232
                     /gene="WASHC2C"
                     /gene_synonym="FAM21A; FAM21C; VPEF"
                     /inference="alignment:Splign:2.1.0"
     exon            1233..1292
                     /gene="WASHC2C"
                     /gene_synonym="FAM21A; FAM21C; VPEF"
                     /inference="alignment:Splign:2.1.0"
     exon            1293..1472
                     /gene="WASHC2C"
                     /gene_synonym="FAM21A; FAM21C; VPEF"
                     /inference="alignment:Splign:2.1.0"
     exon            1473..1600
                     /gene="WASHC2C"
                     /gene_synonym="FAM21A; FAM21C; VPEF"
                     /inference="alignment:Splign:2.1.0"
     exon            1601..1687
                     /gene="WASHC2C"
                     /gene_synonym="FAM21A; FAM21C; VPEF"
                     /inference="alignment:Splign:2.1.0"
     exon            1688..1789
                     /gene="WASHC2C"
                     /gene_synonym="FAM21A; FAM21C; VPEF"
                     /inference="alignment:Splign:2.1.0"
     exon            1790..1921
                     /gene="WASHC2C"
                     /gene_synonym="FAM21A; FAM21C; VPEF"
                     /inference="alignment:Splign:2.1.0"
     exon            1922..2091
                     /gene="WASHC2C"
                     /gene_synonym="FAM21A; FAM21C; VPEF"
                     /inference="alignment:Splign:2.1.0"
     exon            2092..2194
                     /gene="WASHC2C"
                     /gene_synonym="FAM21A; FAM21C; VPEF"
                     /inference="alignment:Splign:2.1.0"
     exon            2195..2347
                     /gene="WASHC2C"
                     /gene_synonym="FAM21A; FAM21C; VPEF"
                     /inference="alignment:Splign:2.1.0"
     exon            2348..2530
                     /gene="WASHC2C"
                     /gene_synonym="FAM21A; FAM21C; VPEF"
                     /inference="alignment:Splign:2.1.0"
     exon            2531..2659
                     /gene="WASHC2C"
                     /gene_synonym="FAM21A; FAM21C; VPEF"
                     /inference="alignment:Splign:2.1.0"
     exon            2660..2740
                     /gene="WASHC2C"
                     /gene_synonym="FAM21A; FAM21C; VPEF"
                     /inference="alignment:Splign:2.1.0"
     exon            2741..2863
                     /gene="WASHC2C"
                     /gene_synonym="FAM21A; FAM21C; VPEF"
                     /inference="alignment:Splign:2.1.0"
     exon            2864..2926
                     /gene="WASHC2C"
                     /gene_synonym="FAM21A; FAM21C; VPEF"
                     /inference="alignment:Splign:2.1.0"
     exon            2927..3139
                     /gene="WASHC2C"
                     /gene_synonym="FAM21A; FAM21C; VPEF"
                     /inference="alignment:Splign:2.1.0"
     exon            3140..3760
                     /gene="WASHC2C"
                     /gene_synonym="FAM21A; FAM21C; VPEF"
                     /inference="alignment:Splign:2.1.0"
     exon            3761..3938
                     /gene="WASHC2C"
                     /gene_synonym="FAM21A; FAM21C; VPEF"
                     /inference="alignment:Splign:2.1.0"
     exon            3939..4642
                     /gene="WASHC2C"
                     /gene_synonym="FAM21A; FAM21C; VPEF"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4262..4267
                     /regulatory_class="polyA_signal_sequence"
                     /gene="WASHC2C"
                     /gene_synonym="FAM21A; FAM21C; VPEF"
                     /note="hexamer: AATAAA"
     polyA_site      4279
                     /gene="WASHC2C"
                     /gene_synonym="FAM21A; FAM21C; VPEF"
     regulatory      4615..4620
                     /regulatory_class="polyA_signal_sequence"
                     /gene="WASHC2C"
                     /gene_synonym="FAM21A; FAM21C; VPEF"
                     /note="hexamer: AATAAA"
     polyA_site      4642
                     /gene="WASHC2C"
                     /gene_synonym="FAM21A; FAM21C; VPEF"
                     /note="major polyA site"
ORIGIN      
        1 ggtcctcggc ctgtgctggc agcctcggag cccaccgagc cgggcggctg ggatgatgaa
       61 ccggacgacc cccgaccagg agctggtgcc ggcgtcggag cccgtgtggg agcggccgtg
      121 gtcggtggag gagatccgca ggagcagcca gagctggtcg ctggcggccg acgcgggcct
      181 actacagttt ctacaggaat tctcacagca aactatctct aggacccatg aaatcaagaa
      241 acaagtggac ggactaatcc gggaaaccaa agccacagat tgtcgcctgc ataatgtctt
      301 caatgacttc cttatgctct ctaataccca gttcatagag aatcgtgtat atgatgaaga
      361 agtggaggag ccagtactca aggctgaggc agaaaaaaca gagcaggaga agacacgaga
      421 gcagaaagaa gtagatctca ttcctaaagt ccaggaggct gtgaactatg gcttacaagt
      481 attggacagt gcctttgagc aacttgatat caaagcagga aactcagact ccgaggaaga
      541 tgatgctaat gggcgggtgg aactgatcct tgaaccaaag gatctataca ttgatcgtcc
      601 tttaccatat ctcattgggt caaagctgtt catggaacaa gaagatgtag gtcttggaga
      661 gctgtccagt gaagaaggct ctgtaggcag tgatcgtggc agtattgtgg acactgagga
      721 agagaaagaa gaggaggagt cagatgaaga ttttgcccat cacagtgaca atgaacaaaa
      781 ccagcacacc acacaaatga gtgatgagga agaggatgat gatggctgtg acctttttgc
      841 tgactctgag aaggaggagg aagatattga ggacattgaa gaaaatacta gacctaaaag
      901 aagcagacct acatcgtttg cagatgagct ggctgcccgc atcaaggggg atgccatggg
      961 tcgagtggac gaggagccga caaccttacc ctcaggagaa gcaaaacctc ggaagacact
     1021 caaagagaag aaggaaagga gaactccttc agacgatgaa gaggataact tattcgcacc
     1081 ccccaagctg accgacgagg acttctcgcc atttggctct ggaggtggcc tgttcagtgg
     1141 cggcaagggg ctatttgatg atgaggacga ggagagtgac ctcttcacgg aagcctccca
     1201 ggatcggcaa gctggagcct ctgttaagga ggagtcttca tcatccaaac ctggaaagaa
     1261 aatcccagca ggagctgttt ctgtattttt aggagacacg gatgtgtttg gtgctgcctc
     1321 cgttccatca ctgaaggagc cacagaagcc tgagcagccc actccaagga aaagccccta
     1381 tggtccccct cccactggcc tctttgatga tgatgatggt gatgatgatg acgacttttt
     1441 ctcggcaccc cacagcaaac cttctaaaac acgcaaagtc caatccactg ccgatatctt
     1501 tggtgacgaa gaaggagatc tgttcaaaga aaaagccgta gcatcgccag aagccactgt
     1561 gagtcagaca gatgaaaata aagcaagagc agaaaaaaag gttaccttat cttacagcaa
     1621 aaatctcaag ccctcatcag aaacaaagac tcaaaaaggc ttattttcag atgaggagga
     1681 ctctgaggat ttgttttctt ctcaaagtgc gagtaactta aaaggtgcat ctctgctgcc
     1741 tggcaagctc cccacgtcgg tttccctgtt tgatgatgaa gatgaagagg ataatctttt
     1801 tgggggtaca gctgctaaga agcagacatt gtctctacaa gctcagagag aagagaaagc
     1861 aaaagcctcc gagctctcca aaaagaaagc atctgccctg ttgttcagca gtgatgagga
     1921 ggaccagtgg aatattcctg cttcacagac ccacttagca tctgacagca ggtctaaagg
     1981 agaacccagg gattctggga ccctccagag ccaggaggcc aaggctgtga aaaagaccag
     2041 tctctttgag gaagacaaag aagatgatct ttttgccatt gccaaggaca gccaaaagaa
     2101 gacccagaga gtgtcactcc tctttgaaga cgatgttgat agcggaggct ctctgtttgg
     2161 ctctcctccc acatctgttc ctcctgcaac aaagaaaaaa gagactgtct ctgaggcacc
     2221 acctttgctg ttcagcgatg aagaagagaa ggaggcacaa cttggagtga agtctgtgga
     2281 taagaaggtt gagagtgcca aggagtcatt aaaatttggg agaactgatg tggctgagtc
     2341 agaaaaggaa ggacttttga ctagatctgc tcaggaaaca gtcaagcatt ctgatttatt
     2401 ttcttcatca tccccatggg acaaaggaac caagcctaga accaaaactg ttcttagctt
     2461 gtttgatgag gaagaggata aaatggaaga tcaaaacatt atccaggctc cacagaaaga
     2521 agtaggaaag ggctgcgatc ctgatgccca ccccaagagc acaggtgtct tccaggatga
     2581 agagctgctt ttcagccaca agctccaaaa ggacaatgac ccagatgttg acctttttgc
     2641 tggcaccaaa aaaaccaagc tgttagagcc aagtgttggg agcctgtttg gggatgatga
     2701 agatgatgat cttttcagct ctgccaagtc ccagcctttg gtacaagaga aaaagagagt
     2761 agtgaaaaaa gaccactctg ttaactcttt caaaaaccag aaacatcctg aatccattca
     2821 aggtagtaaa gaaaaaggca tatggaagcc ggaaacacct caggactcat caggtctcgc
     2881 tccatttaaa accaaagaac catccactcg gatcgggaag atacaagcaa atttagcgat
     2941 caacccagcg gccttgctgc ccacagcggc ttcccagatc tctgaagtaa agcctgtttt
     3001 gccagaattg gcttttcctt catctgaaca cagaaggagc cacggtctgg aaagtgtgcc
     3061 tgtccttccc gggagtgggg aggccggtgt gagttttgat cttccagctc aggcagacac
     3121 cttacacagt gcaaacaaga gccgtgtcaa gatgagaggg aagcgtagac cgcagacccg
     3181 tgcagctagg cggctggctg ctcaggagtc cagcgaggct gaggacatga gcgtccccag
     3241 aggacccatt gcacagtggg ctgatggcgc catttcccca aatggccatc ggccacagct
     3301 cagagcagcc agtggagaag acagcactga ggaggccctg gcagctgccg ctgcaccttg
     3361 ggaaggtggt cctgtgcctg gagtggacac aagccccttt gcaaagtctc tgggtcattc
     3421 cagaggggag gctgaccttt ttgattctgg ggacattttt tccacgggca ctggatctca
     3481 gtccgtggag agaacaaaac ccaaggcaaa gatagcagag aatcctgcca acccaccagt
     3541 gggtggtaaa gcaaagagcc ccatgtttcc tgctctaggc gaggccagca gtgatgatga
     3601 tctctttcag tctgctaaac caaaaccagc aaagaaaaca aatccctttc ctctcctgga
     3661 agatgaggat gacctcttta cagatcagaa agtcaagaag aatgagacaa aatccagtag
     3721 tcagcaggat gtcatattaa caacacaaga tatttttgag gatgatatat ttgctacgga
     3781 agcaattaaa ccctctcaga aaaccagaga gaaggagaaa acattggaat ctaatttatt
     3841 tgatgataac attgatatct ttgctgactt aactgtaaaa ccaaaagaaa agtccaaaaa
     3901 gaaagtggaa gccaagtcta tatttgatga tgatatggat gacatcttct ccactggtat
     3961 ccaggctaag acaaccaaac caaaaagccg atctgcacag gccgcacctg aaccaagatt
     4021 tgaacacaag gtgtccaaca tctttgatga tcccctgaat gcctttggag gccagtagag
     4081 cacacagggt atccacatgt taccctgcag ctacattgtt gagttagtga tgatattgta
     4141 tatgctcatg gtcttaactg gattacaaaa agcaaatact agaacagcta gctcatcttt
     4201 tacccaatgt acttagtatt tttctgcact ggtttaatca tgcttaatac tacaaaacaa
     4261 aaataaatat ttcacagtgg ttggtttgtt ttgtttttaa accacagttt gatttagtta
     4321 gccttgctgg ggccataata tgcttcaggg tgtgtaaaag aagaaatctc tttgtggctt
     4381 tcatgggcag ggaatctcag agatagcaaa tgccacctga ccagaagtct ttgttatatg
     4441 gatgggaacc ctaacttagg gcttgggcag gggaaagaga aagaagatga gagattatac
     4501 ttcatgagtc ttagcaatat gggagcaggt tttcactgaa ttctgagggt gcctctgcat
     4561 gtcctccaag gcaaagtttg gcaaactgtg gcccccccac tgtcatattt tgttaataaa
     4621 attttattgg aacacaatca ca
//
