LOCUS       NM_001005751            4642 bp    mRNA    linear   PRI 11-FEB-2025
DEFINITION  Homo sapiens WASH complex subunit 2A (WASHC2A), transcript variant
            1, mRNA.
ACCESSION   NM_001005751 XM_350780
VERSION     NM_001005751.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4642)
  AUTHORS   Wang,T., Zheng,A.X., Li,P., Tang,T., Zhang,L.P., Hong,Y., Hong,X.
            and Deng,Z.H.
  TITLE     FAM21 interacts with Ku to promote the localization of WASH to DNA
            double strand break sites
  JOURNAL   DNA Repair (Amst) 133, 103603 (2024)
   PUBMED   38029687
  REMARK    GeneRIF: FAM21 interacts with Ku to promote the localization of
            WASH to DNA double strand break sites.
REFERENCE   2  (bases 1 to 4642)
  AUTHORS   Aleksic,T., Gray,N., Wu,X., Rieunier,G., Osher,E., Mills,J.,
            Verrill,C., Bryant,R.J., Han,C., Hutchinson,K., Lambert,A.G.,
            Kumar,R., Hamdy,F.C., Weyer-Czernilofsky,U., Sanderson,M.P.,
            Bogenrieder,T., Taylor,S. and Macaulay,V.M.
  TITLE     Nuclear IGF1R Interacts with Regulatory Regions of Chromatin to
            Promote RNA Polymerase II Recruitment and Gene Expression
            Associated with Advanced Tumor Stage
  JOURNAL   Cancer Res 78 (13), 3497-3509 (2018)
   PUBMED   29735545
  REMARK    GeneRIF: Study reports that nuclear IGF1R associates with advanced
            tumor stage and is recruited selectively to regulatory regions of
            chromatin including JUN and FAM21A promoters. JUN and FAM21A
            identified as mediators of cell survival and IGF-induced migration,
            properties that tumors require to attain advanced stage.
REFERENCE   3  (bases 1 to 4642)
  AUTHORS   Shin,J.J.H., Gillingham,A.K., Begum,F., Chadwick,J. and Munro,S.
  TITLE     TBC1D23 is a bridging factor for endosomal vesicle capture by
            golgins at the trans-Golgi
  JOURNAL   Nat Cell Biol 19 (12), 1424-1432 (2017)
   PUBMED   29084197
  REMARK    Erratum:[Nat Cell Biol. 2018 Feb;20(2):222. doi:
            10.1038/s41556-017-0026-7.. PMID: 29311657]
REFERENCE   4  (bases 1 to 4642)
  AUTHORS   McNally,K.E., Faulkner,R., Steinberg,F., Gallon,M., Ghai,R.,
            Pim,D., Langton,P., Pearson,N., Danson,C.M., Nagele,H.,
            Morris,L.L., Singla,A., Overlee,B.L., Heesom,K.J., Sessions,R.,
            Banks,L., Collins,B.M., Berger,I., Billadeau,D.D., Burstein,E. and
            Cullen,P.J.
  TITLE     Retriever is a multiprotein complex for retromer-independent
            endosomal cargo recycling
  JOURNAL   Nat Cell Biol 19 (10), 1214-1225 (2017)
   PUBMED   28892079
REFERENCE   5  (bases 1 to 4642)
  AUTHORS   Lee,S., Chang,J. and Blackstone,C.
  TITLE     FAM21 directs SNX27-retromer cargoes to the plasma membrane by
            preventing transport to the Golgi apparatus
  JOURNAL   Nat Commun 7, 10939 (2016)
   PUBMED   26956659
  REMARK    GeneRIF: SNX27 interaction with FAM21 is required for the precise
            localization of SNX27 at an endosomal subdomain.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 4642)
  AUTHORS   Phillips-Krawczak,C.A., Singla,A., Starokadomskyy,P., Deng,Z.,
            Osborne,D.G., Li,H., Dick,C.J., Gomez,T.S., Koenecke,M.,
            Zhang,J.S., Dai,H., Sifuentes-Dominguez,L.F., Geng,L.N.,
            Kaufmann,S.H., Hein,M.Y., Wallis,M., McGaughran,J., Gecz,J.,
            Sluis,Bv., Billadeau,D.D. and Burstein,E.
  TITLE     COMMD1 is linked to the WASH complex and regulates endosomal
            trafficking of the copper transporter ATP7A
  JOURNAL   Mol Biol Cell 26 (1), 91-103 (2015)
   PUBMED   25355947
REFERENCE   7  (bases 1 to 4642)
  AUTHORS   Derivery,E., Sousa,C., Gautier,J.J., Lombard,B., Loew,D. and
            Gautreau,A.
  TITLE     The Arp2/3 activator WASH controls the fission of endosomes through
            a large multiprotein complex
  JOURNAL   Dev Cell 17 (5), 712-723 (2009)
   PUBMED   19922875
REFERENCE   8  (bases 1 to 4642)
  AUTHORS   Gomez,T.S. and Billadeau,D.D.
  TITLE     A FAM21-containing WASH complex regulates retromer-dependent
            sorting
  JOURNAL   Dev Cell 17 (5), 699-711 (2009)
   PUBMED   19922874
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            DC336167.1, BC082258.1, BC075815.1 and BC065500.1.
            
            On Nov 22, 2018 this sequence version replaced NM_001005751.2.
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803614.134940.1,
                                           SRR1803612.59923.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000282633.10/ ENSP00000282633.5
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-7                 DC336167.1         64-70
            8-3040              BC082258.1         1-3033
            3041-4637           BC075815.1         2609-4205
            4638-4642           BC065500.1         942-946
FEATURES             Location/Qualifiers
     source          1..4642
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="10"
                     /map="10q11.23"
     gene            1..4642
                     /gene="WASHC2A"
                     /gene_synonym="bA56A21.1; bA98I6.1; FAM21A; FAM21B"
                     /note="WASH complex subunit 2A"
                     /db_xref="GeneID:387680"
                     /db_xref="HGNC:HGNC:23416"
     exon            1..55
                     /gene="WASHC2A"
                     /gene_synonym="bA56A21.1; bA98I6.1; FAM21A; FAM21B"
                     /inference="alignment:Splign:2.1.0"
     CDS             53..4078
                     /gene="WASHC2A"
                     /gene_synonym="bA56A21.1; bA98I6.1; FAM21A; FAM21B"
                     /note="isoform 1 is encoded by transcript variant 1; WASH
                     complex subunit FAM21A; family with sequence similarity
                     21, member B; WASH complex subunit FAM21B; alternative
                     protein FAM21B; family with sequence similarity 21 member
                     A"
                     /codon_start=1
                     /product="WASH complex subunit 2A isoform 1"
                     /protein_id="NP_001005751.1"
                     /db_xref="CCDS:CCDS41527.1"
                     /db_xref="GeneID:387680"
                     /db_xref="HGNC:HGNC:23416"
                     /translation="MMNRTTPDQELAPASEPVWERPWSVEEIRRSSQSWSLAADAGLL
                     QFLQEFSQQTISRTHEIKKQVDGLIRETKATDCRLHNVFNDFLMLSNTQFIENRVYDE
                     EVEEPVLKAEAEKTEQEKTREQKEVDLIPKVQEAVNYGLQVLDSAFEQLDIKAGNSDS
                     EEDDANGRVELILEPKDLYIDRPLPYLIGSKLFMEQEDVGLGELSSEEGSVGSDRGSI
                     VDTEEEKEEEESDEDFAHHSDNEQNRHTTQMSDEEEDDDGCDLFADSEKEEEDIEDIE
                     ENTRPKRSRPTSFADELAARIKGDAVGRVDEEPTTLPSGEAKPRKTLKEKKERRTPSD
                     DEEDNLFAPPKLTDEDFSPFGSGGGLFSGGKGLFDDEDEESDLFTEAPQDRQAGASVK
                     EESSSSKPGKKIPAGAVSVFLGDTDVFGAASVPSMKEPQKPEQPTPRKSPYGPPPTGL
                     FDDDDGDDDDDFFSAPHSKPSKTGKVQSTADIFGDEEGDLFKEKAVASPEATVSQTDE
                     NKARAEKKVTLSSSKNLKPSSETKTQKGLFSDEEDSEDLFSSQSASKLKGASLLPGKL
                     PTLVSLFDDEDEEDNLFGGTAAKKQTLCLQAQREEKAKASELSKKKASALLFSSDEED
                     QWNIPASQTHLASDSRSKGEPRDSGTLQSQEAKAVKKTSLFEEDEEDDLFAIAKDSQK
                     KTQRVSLLFEDDVDSGGSLFGSPPTSVPPATKKKETVSEAPPLLFSDEEEKEAQLGVK
                     SVDKKVESAKESLKFGRTDVAESEKEGLLTRSAQETVKHSDLFSSSSPWDKGTKPRTK
                     TVLSLFDEEEDKMEDQNIIQAPQKEVGKGRDPDAHPKSTGVFQDEELLFSHKLQKDND
                     PDVDLFAGTKKTKLLEPSVGSLFGDDEDDDLFSSAKSQPLVQEKKRVVKKDHSVDSFK
                     NQKHPESIQGSKEKGIWKPETPQDSSGLAPFKTKEPSTRIGKIQANLAINPAALLPTA
                     ASQISEVKPVLPELAFPSSEHRRSHGLESVPVLPGSGEAGVSFDLPAQADTLHSANKS
                     RVKMRGKRRPQTRAARRLAAQESSETEDMSVPRGPIAQWADGAISPNGHRPQLRAASG
                     EDSTEEALAAAAAPWEGGPVPGVDRSPFAKSLGHSRGEADLFDSGDIFSTGTGSQSVE
                     RTKPKAKIAENPANPPVGGKAKSPMFPALGEASSDDDLFQSAKPKPAKKTNPFPLLED
                     EDDLFTDQKVKKNETKSNSQQDVILTTQDIFEDDIFATEAIKPSQKTREKEKTLESNL
                     FDDNIDIFADLTVKPKEKSKKKVEAKSIFDDDMDDIFSSGIQAKTTKPKSRSAQAAPE
                     PRFEHKVSNIFDDPLNAFGGQ"
     exon            56..178
                     /gene="WASHC2A"
                     /gene_synonym="bA56A21.1; bA98I6.1; FAM21A; FAM21B"
                     /inference="alignment:Splign:2.1.0"
     exon            179..343
                     /gene="WASHC2A"
                     /gene_synonym="bA56A21.1; bA98I6.1; FAM21A; FAM21B"
                     /inference="alignment:Splign:2.1.0"
     exon            344..406
                     /gene="WASHC2A"
                     /gene_synonym="bA56A21.1; bA98I6.1; FAM21A; FAM21B"
                     /inference="alignment:Splign:2.1.0"
     exon            407..580
                     /gene="WASHC2A"
                     /gene_synonym="bA56A21.1; bA98I6.1; FAM21A; FAM21B"
                     /inference="alignment:Splign:2.1.0"
     exon            581..674
                     /gene="WASHC2A"
                     /gene_synonym="bA56A21.1; bA98I6.1; FAM21A; FAM21B"
                     /inference="alignment:Splign:2.1.0"
     exon            675..736
                     /gene="WASHC2A"
                     /gene_synonym="bA56A21.1; bA98I6.1; FAM21A; FAM21B"
                     /inference="alignment:Splign:2.1.0"
     exon            737..784
                     /gene="WASHC2A"
                     /gene_synonym="bA56A21.1; bA98I6.1; FAM21A; FAM21B"
                     /inference="alignment:Splign:2.1.0"
     exon            785..895
                     /gene="WASHC2A"
                     /gene_synonym="bA56A21.1; bA98I6.1; FAM21A; FAM21B"
                     /inference="alignment:Splign:2.1.0"
     exon            896..983
                     /gene="WASHC2A"
                     /gene_synonym="bA56A21.1; bA98I6.1; FAM21A; FAM21B"
                     /inference="alignment:Splign:2.1.0"
     exon            984..1055
                     /gene="WASHC2A"
                     /gene_synonym="bA56A21.1; bA98I6.1; FAM21A; FAM21B"
                     /inference="alignment:Splign:2.1.0"
     exon            1056..1174
                     /gene="WASHC2A"
                     /gene_synonym="bA56A21.1; bA98I6.1; FAM21A; FAM21B"
                     /inference="alignment:Splign:2.1.0"
     exon            1175..1232
                     /gene="WASHC2A"
                     /gene_synonym="bA56A21.1; bA98I6.1; FAM21A; FAM21B"
                     /inference="alignment:Splign:2.1.0"
     exon            1233..1292
                     /gene="WASHC2A"
                     /gene_synonym="bA56A21.1; bA98I6.1; FAM21A; FAM21B"
                     /inference="alignment:Splign:2.1.0"
     exon            1293..1472
                     /gene="WASHC2A"
                     /gene_synonym="bA56A21.1; bA98I6.1; FAM21A; FAM21B"
                     /inference="alignment:Splign:2.1.0"
     exon            1473..1600
                     /gene="WASHC2A"
                     /gene_synonym="bA56A21.1; bA98I6.1; FAM21A; FAM21B"
                     /inference="alignment:Splign:2.1.0"
     exon            1601..1687
                     /gene="WASHC2A"
                     /gene_synonym="bA56A21.1; bA98I6.1; FAM21A; FAM21B"
                     /inference="alignment:Splign:2.1.0"
     exon            1688..1789
                     /gene="WASHC2A"
                     /gene_synonym="bA56A21.1; bA98I6.1; FAM21A; FAM21B"
                     /inference="alignment:Splign:2.1.0"
     exon            1790..1921
                     /gene="WASHC2A"
                     /gene_synonym="bA56A21.1; bA98I6.1; FAM21A; FAM21B"
                     /inference="alignment:Splign:2.1.0"
     exon            1922..2091
                     /gene="WASHC2A"
                     /gene_synonym="bA56A21.1; bA98I6.1; FAM21A; FAM21B"
                     /inference="alignment:Splign:2.1.0"
     exon            2092..2194
                     /gene="WASHC2A"
                     /gene_synonym="bA56A21.1; bA98I6.1; FAM21A; FAM21B"
                     /inference="alignment:Splign:2.1.0"
     exon            2195..2347
                     /gene="WASHC2A"
                     /gene_synonym="bA56A21.1; bA98I6.1; FAM21A; FAM21B"
                     /inference="alignment:Splign:2.1.0"
     exon            2348..2530
                     /gene="WASHC2A"
                     /gene_synonym="bA56A21.1; bA98I6.1; FAM21A; FAM21B"
                     /inference="alignment:Splign:2.1.0"
     exon            2531..2659
                     /gene="WASHC2A"
                     /gene_synonym="bA56A21.1; bA98I6.1; FAM21A; FAM21B"
                     /inference="alignment:Splign:2.1.0"
     exon            2660..2740
                     /gene="WASHC2A"
                     /gene_synonym="bA56A21.1; bA98I6.1; FAM21A; FAM21B"
                     /inference="alignment:Splign:2.1.0"
     exon            2741..2863
                     /gene="WASHC2A"
                     /gene_synonym="bA56A21.1; bA98I6.1; FAM21A; FAM21B"
                     /inference="alignment:Splign:2.1.0"
     exon            2864..2926
                     /gene="WASHC2A"
                     /gene_synonym="bA56A21.1; bA98I6.1; FAM21A; FAM21B"
                     /inference="alignment:Splign:2.1.0"
     exon            2927..3139
                     /gene="WASHC2A"
                     /gene_synonym="bA56A21.1; bA98I6.1; FAM21A; FAM21B"
                     /inference="alignment:Splign:2.1.0"
     exon            3140..3760
                     /gene="WASHC2A"
                     /gene_synonym="bA56A21.1; bA98I6.1; FAM21A; FAM21B"
                     /inference="alignment:Splign:2.1.0"
     exon            3761..3938
                     /gene="WASHC2A"
                     /gene_synonym="bA56A21.1; bA98I6.1; FAM21A; FAM21B"
                     /inference="alignment:Splign:2.1.0"
     exon            3939..4642
                     /gene="WASHC2A"
                     /gene_synonym="bA56A21.1; bA98I6.1; FAM21A; FAM21B"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4262..4267
                     /regulatory_class="polyA_signal_sequence"
                     /gene="WASHC2A"
                     /gene_synonym="bA56A21.1; bA98I6.1; FAM21A; FAM21B"
                     /note="hexamer: AATAAA"
     polyA_site      4279
                     /gene="WASHC2A"
                     /gene_synonym="bA56A21.1; bA98I6.1; FAM21A; FAM21B"
                     /note="major polyA site"
     regulatory      4615..4620
                     /regulatory_class="polyA_signal_sequence"
                     /gene="WASHC2A"
                     /gene_synonym="bA56A21.1; bA98I6.1; FAM21A; FAM21B"
                     /note="hexamer: AATAAA"
     polyA_site      4642
                     /gene="WASHC2A"
                     /gene_synonym="bA56A21.1; bA98I6.1; FAM21A; FAM21B"
                     /note="major polyA site"
ORIGIN      
        1 agtcctcggc gtgtgctggc agcttcggag cccaccgagc cgggcggcta ggatgatgaa
       61 ccggacgacc cccgaccagg agctggcgcc agcgtcggag cccgtgtggg agcggccgtg
      121 gtcggtggag gagatccgca ggagcagcca gagctggtcg ctggcggccg acgcgggcct
      181 actacagttt ctacaggaat tctcacagca aactatctct aggacccatg aaatcaagaa
      241 acaagtggac ggactaattc gggaaaccaa agccacagat tgtcgcctgc ataatgtctt
      301 caatgacttc cttatgctct ctaataccca gttcattgag aatcgtgtat atgatgaaga
      361 agtggaggag ccagtactca aggctgaggc agaaaaaaca gagcaggaga agacacgaga
      421 gcagaaagaa gtagatctca ttcctaaagt ccaggaggct gtgaactatg gcttacaagt
      481 attggacagt gcctttgagc aacttgatat caaagcagga aactcagact ccgaggaaga
      541 tgatgctaat gggcgggtgg aactgatcct tgaaccaaag gatctataca ttgatcgtcc
      601 tttaccatat ctcattgggt caaagctgtt catggaacaa gaagatgtag gtcttggaga
      661 gctgtccagt gaagaaggct ctgtaggcag tgatcgtggc agtattgtgg acactgagga
      721 agagaaagaa gaggaggagt cagatgaaga ttttgcccat catagtgaca atgaacaaaa
      781 ccggcacacc acacaaatga gtgatgagga agaggatgat gatggctgtg accttttcgc
      841 tgactctgag aaggaggagg aagatattga ggacattgaa gaaaatacta gacctaaaag
      901 aagcagacct acatcgtttg cagatgagct ggctgcccgc atcaaggggg atgccgtggg
      961 tcgagtggac gaggagccga caaccttacc ctcaggagaa gcaaaacctc ggaagacact
     1021 caaagagaag aaggaaagga gaactccttc agacgatgaa gaggataact tattcgcacc
     1081 ccccaagctg accgacgagg acttctcgcc atttggctct ggaggtggcc tgttcagtgg
     1141 gggcaagggg ctctttgatg atgaggacga ggagagtgac ctcttcacgg aagcccccca
     1201 ggatcggcaa gctggagcct ctgttaagga ggagtcttca tcatccaaac ctggaaagaa
     1261 aatcccagca ggagctgttt ctgtattttt aggagacacg gatgtgtttg gtgctgcctc
     1321 cgttccatca atgaaggagc cacagaagcc tgagcagccc actccaagga aaagccccta
     1381 tggtccccct cccactggcc tctttgatga tgatgatggt gatgatgatg acgacttttt
     1441 ctcggcaccc cacagcaaac cttctaaaac aggcaaagtc caatccactg ccgatatctt
     1501 tggtgacgaa gaaggagatc tgttcaaaga aaaagccgta gcatcgccag aagccactgt
     1561 gagtcagaca gatgaaaata aagcaagagc agaaaaaaag gttaccttat cttccagcaa
     1621 aaatctcaag ccctcatcag aaacaaagac tcaaaaaggc ttattttcag atgaggagga
     1681 ctctgaggat ttgttttctt ctcaaagtgc gagtaagtta aaaggtgcgt ctctgctgcc
     1741 tggcaagctc cccacgttgg tttccctgtt tgatgatgaa gatgaagagg ataatctttt
     1801 tgggggtaca gctgctaaga agcagacatt gtgtctacaa gctcagagag aagagaaagc
     1861 aaaagcctcc gagctctcca aaaagaaagc atctgccctg ttgttcagca gtgatgagga
     1921 ggaccagtgg aatattcctg cttcacagac ccacttagca tctgacagca ggtctaaagg
     1981 agaacccagg gattctggga ccctccagag ccaggaggcc aaggctgtga aaaagaccag
     2041 tctctttgag gaagacgaag aagatgatct ttttgccatt gccaaggaca gccaaaagaa
     2101 gacccagaga gtgtcactcc tctttgaaga cgatgttgat agcggaggct ctctgtttgg
     2161 ctctcctccc acatctgttc ctcctgcaac aaagaaaaaa gagactgtct ctgaggcacc
     2221 acctttgctg ttcagcgatg aagaagagaa ggaggcacaa cttggagtga agtctgtgga
     2281 taagaaggtt gagagtgcca aggagtcatt aaaatttggg agaactgatg tggctgagtc
     2341 agaaaaggaa ggacttttga ctagatctgc tcaggaaaca gtcaagcatt ctgatttatt
     2401 ttcttcatca tccccatggg acaaaggaac caagcctaga accaaaactg ttcttagctt
     2461 gtttgatgag gaagaggata aaatggaaga tcaaaacatt atccaggctc cacagaaaga
     2521 agtaggaaag ggccgcgatc ctgatgccca ccccaagagc acaggtgtct tccaggatga
     2581 agagctgctt ttcagccaca agctccaaaa ggacaatgac ccagatgttg acctttttgc
     2641 tggcaccaaa aaaaccaagc tgttagagcc aagtgttggg agcctgtttg gggatgatga
     2701 agatgatgat cttttcagct ctgccaagtc ccagcctttg gtacaagaga aaaagagagt
     2761 agtgaaaaaa gaccactctg ttgactcttt caaaaaccag aaacatcctg aatccattca
     2821 aggtagtaaa gaaaaaggca tatggaagcc ggaaacacct caggactcat caggtctcgc
     2881 tccatttaaa accaaagaac catccactcg gatcgggaag atacaagcaa atttagcgat
     2941 caacccagcg gccttgctgc ccacagcggc ttcccagatc tctgaagtaa agcctgtttt
     3001 gccagaattg gcttttcctt catctgaaca cagaaggagc cacggtctgg aaagtgtgcc
     3061 tgtccttccc gggagtgggg aggccggtgt gagttttgat cttccagctc aggcagacac
     3121 cttacacagt gcaaacaaga gccgtgtcaa gatgagaggg aagcgtagac cgcagacccg
     3181 tgcagctagg cggctggctg ctcaggagtc cagcgagact gaggacatga gcgtccccag
     3241 aggacccatt gcacagtggg ctgatggcgc catttcccca aatggccatc ggccacagct
     3301 cagagcagcc agtggagaag acagcactga ggaggccctg gcagctgccg ctgcaccttg
     3361 ggaaggtggt cctgtgcctg gagtggacag aagccccttt gcaaagtctc tgggtcattc
     3421 cagaggggag gctgaccttt ttgattctgg ggacatcttt tccacgggca ctggatctca
     3481 gtctgtggag agaacaaaac ccaaggcaaa gatagcagag aatcctgcca acccaccagt
     3541 gggtggtaaa gcaaagagcc ccatgtttcc tgctctaggt gaggccagca gtgatgatga
     3601 tctctttcag tctgctaaac caaaaccagc aaagaaaaca aatccctttc ctctcctgga
     3661 agatgaggat gacctcttta cagatcagaa agtcaagaag aatgagacaa aatccaatag
     3721 tcagcaggat gtcatattaa caacacaaga tatttttgag gatgatatat ttgctacgga
     3781 agcaattaaa ccctctcaga aaaccagaga gaaggagaaa acattggaat ctaatttatt
     3841 tgatgataac attgatatct ttgctgactt aactgtaaaa ccaaaagaaa agtccaaaaa
     3901 gaaagtggaa gccaagtcta tatttgatga tgatatggat gacatcttct cctctggtat
     3961 ccaggctaag acaaccaaac caaaaagccg atctgcacag gccgcacctg aaccaagatt
     4021 tgaacacaag gtgtccaaca tctttgatga tcccctgaat gcctttggag gccagtagag
     4081 cacacagggt atccacatgt taccctgcag ctacattgtt gagttagtga tgatgttgta
     4141 tatgctgatg gtcttaactg gattacaaaa agcaaatact agaacagcta gctcatcgtt
     4201 cacccaatgt acttggtatt tttctgcact ggtttaatca tgcttaatac tacaaaacaa
     4261 aaataaatat ttcacagtgg ttggtttgtt ttgtttttaa accacagttt gatttagtta
     4321 gccttgctgg ggccataata tgcttcaggg tgtgtaaaag aagaaatctc tttgtggctt
     4381 tcatgggcag ggaatcccag agatagcaaa tgccacctga ccagaagtct ttgttatatg
     4441 gatgggaacc ctaacttagg gcctgggcag gggaaagaga aagaaggtga gagattatac
     4501 ttcatgagtc ttagcaatat gggagcaggt tttcactgaa ttctgagggt gcctctgcat
     4561 gtcctccaag gcaaagtttg gcaaactgtg gcccccccac tgtcatattt tgttaataaa
     4621 attttattgg aacacaacca ca
//
