LOCUS       NM_006646               4857 bp    mRNA    linear   PRI 11-FEB-2025
DEFINITION  Homo sapiens WASP family member 3 (WASF3), transcript variant 1,
            mRNA.
ACCESSION   NM_006646
VERSION     NM_006646.6
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4857)
  AUTHORS   Wang,W., Zhang,C., Xiong,M., Jiang,L., Fang,Z., Zhou,H. and Shao,Y.
  TITLE     WAVE3 Facilitates the Tumorigenesis and Metastasis of Tongue
            Squamous Cell Carcinoma via EMT
  JOURNAL   Appl Biochem Biotechnol 196 (7), 4287-4302 (2024)
   PUBMED   37947948
  REMARK    GeneRIF: WAVE3 Facilitates the Tumorigenesis and Metastasis of
            Tongue Squamous Cell Carcinoma via EMT.
REFERENCE   2  (bases 1 to 4857)
  AUTHORS   Wang,W., Rana,P.S., Markovic,V. and Sossey-Alaoui,K.
  TITLE     The WAVE3/beta-catenin oncogenic signaling regulates
            chemoresistance in triple negative breast cancer
  JOURNAL   Breast Cancer Res 25 (1), 31 (2023)
   PUBMED   36949468
  REMARK    GeneRIF: The WAVE3/beta-catenin oncogenic signaling regulates
            chemoresistance in triple negative breast cancer.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4857)
  AUTHORS   Limaye,A.J., Whittaker,M.K., Bendzunas,G.N., Cowell,J.K. and
            Kennedy,E.J.
  TITLE     Targeting the WASF3 complex to suppress metastasis
  JOURNAL   Pharmacol Res 182, 106302 (2022)
   PUBMED   35691539
  REMARK    GeneRIF: Targeting the WASF3 complex to suppress metastasis.
            Review article
REFERENCE   4  (bases 1 to 4857)
  AUTHORS   Teng,Y., Loveless,R., Benson,E.M., Sun,L., Shull,A.Y. and Shay,C.
  TITLE     SHOX2 cooperates with STAT3 to promote breast cancer metastasis
            through the transcriptional activation of WASF3
  JOURNAL   J Exp Clin Cancer Res 40 (1), 274 (2021)
   PUBMED   34465361
  REMARK    GeneRIF: SHOX2 cooperates with STAT3 to promote breast cancer
            metastasis through the transcriptional activation of WASF3.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 4857)
  AUTHORS   Kansakar,U., Wang,W., Markovic,V. and Sossey-Alaoui,K.
  TITLE     Phosphorylation of the proline-rich domain of WAVE3 drives its
            oncogenic activity in breast cancer
  JOURNAL   Sci Rep 11 (1), 3868 (2021)
   PUBMED   33594155
  REMARK    GeneRIF: Phosphorylation of the proline-rich domain of WAVE3 drives
            its oncogenic activity in breast cancer.
            Erratum:[Sci Rep. 2021 Jul 29;11(1):15800. doi:
            10.1038/s41598-021-94981-0.. PMID: 34326443]
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 4857)
  AUTHORS   Marchand,J.B., Kaiser,D.A., Pollard,T.D. and Higgs,H.N.
  TITLE     Interaction of WASP/Scar proteins with actin and vertebrate Arp2/3
            complex
  JOURNAL   Nat Cell Biol 3 (1), 76-82 (2001)
   PUBMED   11146629
REFERENCE   7  (bases 1 to 4857)
  AUTHORS   Jones,G.E.
  TITLE     Cellular signaling in macrophage migration and chemotaxis
  JOURNAL   J Leukoc Biol 68 (5), 593-602 (2000)
   PUBMED   11073096
  REMARK    Review article
REFERENCE   8  (bases 1 to 4857)
  AUTHORS   Suetsugu,S., Miki,H. and Takenawa,T.
  TITLE     Identification of two human WAVE/SCAR homologues as general actin
            regulatory molecules which associate with the Arp2/3 complex
  JOURNAL   Biochem Biophys Res Commun 260 (1), 296-302 (1999)
   PUBMED   10381382
REFERENCE   9  (bases 1 to 4857)
  AUTHORS   Bear,J.E., Rawls,J.F. and Saxe,C.L. 3rd.
  TITLE     SCAR, a WASP-related protein, isolated as a suppressor of receptor
            defects in late Dictyostelium development
  JOURNAL   J Cell Biol 142 (5), 1325-1335 (1998)
   PUBMED   9732292
REFERENCE   10 (bases 1 to 4857)
  AUTHORS   Schott,D.R., Chang,J.N., Deng,G., Kurisu,W., Kuo,W.L., Gray,J. and
            Smith,H.S.
  TITLE     A candidate tumor suppressor gene in human breast cancers
  JOURNAL   Cancer Res 54 (6), 1393-1396 (1994)
   PUBMED   8137235
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL353789.18, BC050283.1,
            AB020707.2, AL133590.1, BU736040.1 and AL159978.14.
            
            On May 17, 2019 this sequence version replaced NM_006646.5.
            
            Summary: This gene encodes a member of the Wiskott-Aldrich syndrome
            protein family. The gene product is a protein that forms a
            multiprotein complex that links receptor kinases and actin. Binding
            to actin occurs through a C-terminal verprolin homology domain in
            all family members. The multiprotein complex serves to tranduce
            signals that involve changes in cell shape, motility or function. A
            pseudogene of this gene have been defined on chromosome 6.
            Alternative splicing results in multiple transcript variants
            [provided by RefSeq, May 2014].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF454702.1, SRR7346977.1687040.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1968189
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000335327.6/ ENSP00000335055.5
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-20                AL353789.18        131229-131248
            21-139              BC050283.1         1-119
            140-3646            AB020707.2         9-3515
            3647-4818           AL133590.1         1289-2460
            4819-4854           BU736040.1         17-52               c
            4855-4857           AL159978.14        11712-11714
FEATURES             Location/Qualifiers
     source          1..4857
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="13"
                     /map="13q12.13"
     gene            1..4857
                     /gene="WASF3"
                     /gene_synonym="Brush-1; SCAR3; WAVE3"
                     /note="WASP family member 3"
                     /db_xref="GeneID:10810"
                     /db_xref="HGNC:HGNC:12734"
                     /db_xref="MIM:605068"
     exon            1..137
                     /gene="WASF3"
                     /gene_synonym="Brush-1; SCAR3; WAVE3"
                     /inference="alignment:Splign:2.1.0"
     exon            138..235
                     /gene="WASF3"
                     /gene_synonym="Brush-1; SCAR3; WAVE3"
                     /inference="alignment:Splign:2.1.0"
     exon            236..378
                     /gene="WASF3"
                     /gene_synonym="Brush-1; SCAR3; WAVE3"
                     /inference="alignment:Splign:2.1.0"
     CDS             246..1754
                     /gene="WASF3"
                     /gene_synonym="Brush-1; SCAR3; WAVE3"
                     /note="isoform 1 is encoded by transcript variant 1; WASP
                     family Verprolin-homologous protein 3; wiskott-Aldrich
                     syndrome protein family member 3; protein WAVE-3; WAS
                     protein family member 3; verprolin homology
                     domain-containing protein 3; actin-binding protein WASF3"
                     /codon_start=1
                     /product="actin-binding protein WASF3 isoform 1"
                     /protein_id="NP_006637.2"
                     /db_xref="CCDS:CCDS9318.1"
                     /db_xref="GeneID:10810"
                     /db_xref="HGNC:HGNC:12734"
                     /db_xref="MIM:605068"
                     /translation="MPLVKRNIEPRHLCRGALPEGITSELECVTNSTLAAIIRQLSSL
                     SKHAEDIFGELFNEANNFYIRANSLQDRIDRLAVKVTQLDSTVEEVSLQDINMKKAFK
                     SSTVQDQQVVSKNSIPNPVADIYNQSDKPPPLNILTPYRDDKKDGLKFYTDPSYFFDL
                     WKEKMLQDTEDKRKEKRRQKEQKRIDGTTREVKKVRKARNRRQEWNMMAYDKELRPDN
                     RLSQSVYHGASSEGSLSPDTRSHASDVTDYSYPATPNHSLHPQPVTPSYAAGDVPPHG
                     PASQAAEHEYRPPSASARHMALNRPQQPPPPPPPQAPEGSQASAPMAPADYGMLPAQI
                     IEYYNPSGPPPPPPPPVIPSAQTAFVSPLQMPMQPPFPASASSTHAAPPHPPSTGLLV
                     TAPPPPGPPPPPPGPPGPGSSLSSSPMHGPPVAEAKRQEPAQPPISDARSDLLAAIRM
                     GIQLKKVQEQREQEAKREPVGNDVATILSRRIAVEYSDSDDDSEFDENDWSD"
     exon            379..513
                     /gene="WASF3"
                     /gene_synonym="Brush-1; SCAR3; WAVE3"
                     /inference="alignment:Splign:2.1.0"
     exon            514..667
                     /gene="WASF3"
                     /gene_synonym="Brush-1; SCAR3; WAVE3"
                     /inference="alignment:Splign:2.1.0"
     exon            668..785
                     /gene="WASF3"
                     /gene_synonym="Brush-1; SCAR3; WAVE3"
                     /inference="alignment:Splign:2.1.0"
     exon            786..961
                     /gene="WASF3"
                     /gene_synonym="Brush-1; SCAR3; WAVE3"
                     /inference="alignment:Splign:2.1.0"
     exon            962..1228
                     /gene="WASF3"
                     /gene_synonym="Brush-1; SCAR3; WAVE3"
                     /inference="alignment:Splign:2.1.0"
     exon            1229..1596
                     /gene="WASF3"
                     /gene_synonym="Brush-1; SCAR3; WAVE3"
                     /inference="alignment:Splign:2.1.0"
     exon            1597..4857
                     /gene="WASF3"
                     /gene_synonym="Brush-1; SCAR3; WAVE3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4819..4824
                     /regulatory_class="polyA_signal_sequence"
                     /gene="WASF3"
                     /gene_synonym="Brush-1; SCAR3; WAVE3"
                     /note="hexamer: ATTACA"
     polyA_site      4837
                     /gene="WASF3"
                     /gene_synonym="Brush-1; SCAR3; WAVE3"
                     /note="major polyA site"
ORIGIN      
        1 gtggacgggc cggaggcggc gtcgctcgcg ccgctgcgtg cggtgtggtg cgaggcgggt
       61 gcgccgggcg gccgcggcgc ggcgggacca tggagctcag agcgcagccc cggcgccggc
      121 ggcgggaccg cggaccgttt tagttttgat tgctgttaac tactactgat aactgagcca
      181 aagtggtgat gctacttgag gggaatttct gcaacccata gtcaacgatt acatgattgt
      241 gaaccatgcc tttagtgaag aggaacattg agccccggca cttgtgccgg ggagctctgc
      301 ctgaagggat taccagcgaa cttgaatgtg taaccaatag tactcttgcc gctatcatac
      361 gccagctgag cagtctgagc aaacatgctg aagacatatt tggtgagttg tttaatgagg
      421 ctaacaactt ctacatcaga gcaaattctc ttcaagacag aattgatcgc cttgctgtca
      481 aagtcaccca gctggattca acagtggaag aggtctcact acaggatatc aacatgaaaa
      541 aagctttcaa aagttccaca gtccaagacc agcaagtggt ttcaaagaac agcattccta
      601 atcctgttgc tgatatttac aaccagagtg ataagccacc gcctctgaac atcctgacac
      661 catacagaga tgacaagaag gatgggctga agttctatac tgatccttcc tatttctttg
      721 acctctggaa agaaaaaatg ctacaggaca cagaagacaa aaggaaagag aaaaggcgtc
      781 aaaaggagca aaagcgtata gatggcacca cccgtgaggt gaaaaaggtt agaaaagcca
      841 gaaacaggcg ccaggagtgg aatatgatgg catatgacaa agagcttaga cccgacaaca
      901 ggttgtctca gagtgtgtac catggagcgt cttccgaggg atccctgtcc ccagatacta
      961 ggtcacatgc atcggacgtt acggattact cttacccggc tactcccaac cattctctgc
     1021 acccccagcc tgtgacccct tcctatgcag ctggtgacgt gccaccacac gggcctgcaa
     1081 gccaggctgc ggagcatgag taccggcccc catctgcctc ggcgaggcac atggccctca
     1141 acagacctca gcagccgccc cccccgcctc cccctcaggc cccagagggg tcccaggcct
     1201 ctgcaccgat ggctccagca gactacggga tgctcccagc gcagataatt gagtattaca
     1261 acccatccgg accacctcct ccgccacctc ctcctgtgat tccctcagca caaactgcct
     1321 tcgtcagccc tctccagatg cccatgcagc ccccgttccc tgcatcagcc agctccacgc
     1381 acgcagctcc tcctcaccca ccctccaccg ggctcctggt cacagccccg ccacccccgg
     1441 gcccaccacc tcccccgcca ggccctcctg gtcccgggtc ttctctttcg tcctccccaa
     1501 tgcatggccc cccagtagct gaggcgaagc ggcaagagcc tgcacagcca ccaatcagtg
     1561 atgctcgaag cgacctcctc gctgctattc gaatgggaat tcaactgaaa aaggtgcagg
     1621 agcagcggga gcaggaggcc aagcgggagc cagtggggaa tgacgtggcc acgatcctgt
     1681 cccggcgcat tgccgtggag tacagcgact ctgacgacga ctcagagttc gacgagaacg
     1741 actggtccga ctgagcaaag gccggcggag aggccgcgtg tgggagcgtg ttgaagattt
     1801 taagtggtct ctacacccaa atagtggtat tctaatcccg tagcatagca ccttttgtat
     1861 aaacaatgtg atattgcttc tgcacatcca aaaattctgg gtcttttcag tatttactgt
     1921 gtaatactta agtgccacta aacatagcaa attgtgctgc acatgaggaa ataggctgtc
     1981 actatcacat tgtcctgaaa acagcatctg ctttcctctt ggccatgaga gtatttagtg
     2041 cagtttgggt ttactcttac tgatcaatat aactctgcag acttgctgtg tgtttgtgaa
     2101 gctgcctggt gttaggtctc tgcaagacta atgactatgt cagagtgatg tcttccaacc
     2161 agtaagtgat attgtttcac cgctttggtt tttccttttg tttttttaaa ggatgtgttt
     2221 ctgaataagt tggttttaga gggaaaggtt caacaaacag ggagaatcca gtgtttctgc
     2281 tttcagtttc ttggcttggt agcctctgag tgaatctgat gctctgctga ataatttcat
     2341 tacctctgca catgcctgtc aaatatgaaa ttggaagggc ccttttcagg ctgggttccc
     2401 tgtgggcatt tgcttagtaa atgcctgttg atggtttttg caagagaatt cagcagctca
     2461 acagtaatga aagtgaagaa cagagtccat tttccttcct ggggccattg gggatgacac
     2521 tcaagatact tgccagtctc tccagtgtgg gcaccagccg gccagaacag atgcgagcag
     2581 tccatgactc tgggagctac accgctgagc tgggcagagc tgcggcacag ggcctgggct
     2641 gcaaaggagc cctgctcctt tagtttcctg acacctgtgt cctgagtgag ccgcaggagt
     2701 tcttagctcc tcagcgagca acagagagca ctttagatgg cacctttcac cacttggtca
     2761 gaattttaaa agcttaggtt taggtgaaag tagatattga cagctattca cctttcacgg
     2821 tgctggggcc agattaggga tcactcccgt gaggagggcc ttcaccctgt tctagaagca
     2881 catggttgtc ctcctgttgt tggcacatta aatgataaaa agcacctcat gagattccct
     2941 tgatcagccc tgcagctgta gtacagtgct gtgccctcac ctccaccctt cctgttgttc
     3001 ccacgtgggc aataccaggg acccatgggg aaactcaaga atgacagctt ctatattttg
     3061 taattctgga tgaaagataa ctgtgttgaa caaacaggtg ctccaggctt tgattataga
     3121 tacgacttca aaaatatgct aagacgcttg acttattaag gactttaacc tactcaacag
     3181 tatttcatat ccattgtggt tagttactca gttatgttga gaagaatctg gagctaaaag
     3241 cagagatgtt tgaggtgacg gtaggaatgt gagcaggatg gtgatggggg tttttgttaa
     3301 aatgcatctg agcaagtcag ccagccccga agtcccctca gtgtgtgtgt ctcgagtggc
     3361 tacctgttgg gcttgtgggc agtgattgta cagagcctgt ccattgggtg cagtcatgta
     3421 gatctgaagc cctgaaaagc ctcatgtctg catccccttt ccaaggctgc ttccctggtg
     3481 tgtctgttct ctcctctgtc ccaggtgctg ggagcgtcct cttcagcgtc ttttcctaga
     3541 gctggtcacc actaggctgt cactataaat tccttgaata taagtaacag ttattaatga
     3601 acttctaaat ttctaatttc tctctctctc tctttttttt ttttttgttg ttgttaaaaa
     3661 gggcctacta cattggcgct attcttagga cttctgcaac ttttaaagtc ttacttgtct
     3721 ttcttgttgc ttttgtatta ggagttcccc gtgtgggtct agaactcccc tttggtaatg
     3781 cttctttgtt tttttatggc ccttctgttc tcaggatgga gagaacacag aagctactat
     3841 ccatgtcagg atttattcta tttatatctt attacaataa aattagtggc actttattca
     3901 taaatattca tgagcctgtt aattgttagt tgtcttcctg tagctgaatc aacaagttat
     3961 tttcaactca attttatgac ttgcgaaaaa gcttttgccc tgttgtgtac cataacattt
     4021 aaaagaatgg aaaatgactg aaatccaatt tagattattt ttagagtatt tttccagcaa
     4081 attcaattta ttctgaaatt taaatccaga tcttttctaa tatggtatta caatgaaaag
     4141 aataaagaga agatttgaat tttcagtttc attttcaaaa actatttacc aaaacaaatg
     4201 gagaagaaac atccaaaagc acatttcatt tctccaaact ttgtgtttta aattatagtt
     4261 ataaattgta aggtaatttt aaattgtccc tcgtattatt tctccacgtc tgttttagtt
     4321 taatgtctcc taagcttttc tctcatagcg tagacctagg gaagggatgg gaagattgcc
     4381 cagtccccga tggctgcgca cacaggaggc ggcggagcac aaggcaagtg agtttgcact
     4441 gtcagcccca gaccgtaagc ttggctacac tgatgttttt ctttactaag gatactattc
     4501 aaaaattaac attttcatct cagtaagttt ttagaacatc aaaatgtttt ctgagctcca
     4561 agtggctagg ttgtaaaagt tttataataa tttgcaatta aaatacatga tacatattaa
     4621 tccattaaag actagtggga atgtatcagc cagagtagca agtaattttt gttttataaa
     4681 tcatagtatc tgtcatcttg cagtattacc aatgctgttg taaattgaat ttaaagtggt
     4741 attaaaaaaa actgttaaac aatttttatc tgtttgtata tcttactata gattatgtac
     4801 aagtaacatc taaataaaat tacactttta accctaagag gttttgctta ttaataa
//
