LOCUS       NM_006990               5681 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens WASP family member 2 (WASF2), transcript variant 1,
            mRNA.
ACCESSION   NM_006990
VERSION     NM_006990.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5681)
  AUTHORS   Kliche,J., Garvanska,D.H., Simonetti,L., Badgujar,D.,
            Dobritzsch,D., Nilsson,J., Davey,N.E. and Ivarsson,Y.
  TITLE     Large-scale phosphomimetic screening identifies phospho-modulated
            motif-based protein interactions
  JOURNAL   Mol Syst Biol 19 (7), e11164 (2023)
   PUBMED   37219487
REFERENCE   2  (bases 1 to 5681)
  AUTHORS   Rana,P.S., Wang,W., Markovic,V., Szpendyk,J., Chan,E.R. and
            Sossey-Alaoui,K.
  TITLE     The WAVE2/miR-29/Integrin-beta1 Oncogenic Signaling Axis Promotes
            Tumor Growth and Metastasis in Triple-negative Breast Cancer
  JOURNAL   Cancer Res Commun 3 (1), 160-174 (2023)
   PUBMED   36968231
  REMARK    GeneRIF: The WAVE2/miR-29/Integrin-beta1 Oncogenic Signaling Axis
            Promotes Tumor Growth and Metastasis in Triple-negative Breast
            Cancer.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 5681)
  AUTHORS   Ahn,H.R., Baek,G.O., Yoon,M.G., Son,J.A., Yoon,J.H., Cheong,J.Y.,
            Cho,H.J., Kang,H.C., Eun,J.W. and Kim,S.S.
  TITLE     Hypomethylation-mediated upregulation of the WASF2 promoter region
            correlates with poor clinical outcomes in hepatocellular carcinoma
  JOURNAL   J Exp Clin Cancer Res 41 (1), 158 (2022)
   PUBMED   35477411
  REMARK    GeneRIF: Hypomethylation-mediated upregulation of the WASF2
            promoter region correlates with poor clinical outcomes in
            hepatocellular carcinoma.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 5681)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   5  (bases 1 to 5681)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   6  (bases 1 to 5681)
  AUTHORS   Qualmann,B., Roos,J., DiGregorio,P.J. and Kelly,R.B.
  TITLE     Syndapin I, a synaptic dynamin-binding protein that associates with
            the neural Wiskott-Aldrich syndrome protein
  JOURNAL   Mol Biol Cell 10 (2), 501-513 (1999)
   PUBMED   9950691
REFERENCE   7  (bases 1 to 5681)
  AUTHORS   Machesky,L.M. and Insall,R.H.
  TITLE     Scar1 and the related Wiskott-Aldrich syndrome protein, WASP,
            regulate the actin cytoskeleton through the Arp2/3 complex
  JOURNAL   Curr Biol 8 (25), 1347-1356 (1998)
   PUBMED   9889097
REFERENCE   8  (bases 1 to 5681)
  AUTHORS   Wu,Y., Dowbenko,D. and Lasky,L.A.
  TITLE     PSTPIP 2, a second tyrosine phosphorylated, cytoskeletal-associated
            protein that binds a PEST-type protein-tyrosine phosphatase
  JOURNAL   J Biol Chem 273 (46), 30487-30496 (1998)
   PUBMED   9804817
REFERENCE   9  (bases 1 to 5681)
  AUTHORS   Bear,J.E., Rawls,J.F. and Saxe,C.L. 3rd.
  TITLE     SCAR, a WASP-related protein, isolated as a suppressor of receptor
            defects in late Dictyostelium development
  JOURNAL   J Cell Biol 142 (5), 1325-1335 (1998)
   PUBMED   9732292
REFERENCE   10 (bases 1 to 5681)
  AUTHORS   She,H.Y., Rockow,S., Tang,J., Nishimura,R., Skolnik,E.Y., Chen,M.,
            Margolis,B. and Li,W.
  TITLE     Wiskott-Aldrich syndrome protein is associated with the adapter
            protein Grb2 and the epidermal growth factor receptor in living
            cells
  JOURNAL   Mol Biol Cell 8 (9), 1709-1721 (1997)
   PUBMED   9307968
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DC300388.1, BC040943.1,
            FO393419.1 and AA736923.1.
            
            On Nov 22, 2018 this sequence version replaced NM_006990.4.
            
            Summary: This gene encodes a member of the Wiskott-Aldrich syndrome
            protein family. The gene product is a protein that forms a
            multiprotein complex that links receptor kinases and actin. Binding
            to actin occurs through a C-terminal verprolin homology domain in
            all family members. The multiprotein complex serves to tranduce
            signals that involve changes in cell shape, motility or function.
            The published map location (PMID:10381382) has been changed based
            on recent genomic sequence comparisons, which indicate that the
            expressed gene is located on chromosome 1, and a pseudogene may be
            located on chromosome X. Two transcript variants encoding different
            isoforms have been found for this gene. [provided by RefSeq, Jan
            2011].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR7346977.1621761.1, BC040943.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000618852.5/ ENSP00000483313.1
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-18                DC300388.1         21-38
            19-1463             BC040943.1         1-1445
            1464-1464           FO393419.1         51451-51451
            1465-2104           BC040943.1         1447-2086
            2105-5196           FO393419.1         53437-56528
            5197-5681           AA736923.1         21-505              c
FEATURES             Location/Qualifiers
     source          1..5681
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1p36.11"
     gene            1..5681
                     /gene="WASF2"
                     /gene_synonym="dJ393P12.2; IMD2; SCAR2; WASF4; WAVE2"
                     /note="WASP family member 2"
                     /db_xref="GeneID:10163"
                     /db_xref="HGNC:HGNC:12733"
                     /db_xref="MIM:605875"
     exon            1..182
                     /gene="WASF2"
                     /gene_synonym="dJ393P12.2; IMD2; SCAR2; WASF4; WAVE2"
                     /inference="alignment:Splign:2.1.0"
     exon            183..355
                     /gene="WASF2"
                     /gene_synonym="dJ393P12.2; IMD2; SCAR2; WASF4; WAVE2"
                     /inference="alignment:Splign:2.1.0"
     CDS             226..1722
                     /gene="WASF2"
                     /gene_synonym="dJ393P12.2; IMD2; SCAR2; WASF4; WAVE2"
                     /note="isoform 1 is encoded by transcript variant 1;
                     suppressor of cyclic-AMP receptor (WASP-family);
                     actin-binding protein WASF2; WAS protein family member 2;
                     WASP family Verprolin-homologous protein 2;
                     wiskott-Aldrich syndrome protein family member 2; protein
                     WAVE-2; WASP family protein member 2; verprolin homology
                     domain-containing protein 2; WASP family protein member 4;
                     putative Wiskott-Aldrich syndrome protein family member 4"
                     /codon_start=1
                     /product="actin-binding protein WASF2 isoform 1"
                     /protein_id="NP_008921.1"
                     /db_xref="CCDS:CCDS304.1"
                     /db_xref="GeneID:10163"
                     /db_xref="HGNC:HGNC:12733"
                     /db_xref="MIM:605875"
                     /translation="MPLVTRNIEPRHLCRQTLPSVRSELECVTNITLANVIRQLGSLS
                     KYAEDIFGELFTQANTFASRVSSLAERVDRLQVKVTQLDPKEEEVSLQGINTRKAFRS
                     STIQDQKLFDRNSLPVPVLETYNTCDTPPPLNNLTPYRDDGKEALKFYTDPSYFFDLW
                     KEKMLQDTKDIMKEKRKHRKEKKDNPNRGNVNPRKIKTRKEEWEKMKMGQEFVESKEK
                     LGTSGYPPTLVYQNGSIGCVENVDASSYPPPPQSDSASSPSPSFSEDNLPPPPAEFSY
                     PVDNQRGSGLAGPKRSSVVSPSHPPPAPPLGSPPGPKPGFAPPPAPPPPPPPMIGIPP
                     PPPPVGFGSPGTPPPPSPPSFPPHPDFAAPPPPPPPPAADYPTLPPPPLSQPTGGAPP
                     PPPPPPPPGPPPPPFTGADGQPAIPPPLSDTTKPKSSLPAVSDARSDLLSAIRQGFQL
                     RRVEEQREQEKRDVVGNDVATILSRRIAVEYSDSEDDSSEFDEDDWSD"
     exon            356..490
                     /gene="WASF2"
                     /gene_synonym="dJ393P12.2; IMD2; SCAR2; WASF4; WAVE2"
                     /inference="alignment:Splign:2.1.0"
     exon            491..644
                     /gene="WASF2"
                     /gene_synonym="dJ393P12.2; IMD2; SCAR2; WASF4; WAVE2"
                     /inference="alignment:Splign:2.1.0"
     exon            645..762
                     /gene="WASF2"
                     /gene_synonym="dJ393P12.2; IMD2; SCAR2; WASF4; WAVE2"
                     /inference="alignment:Splign:2.1.0"
     exon            763..893
                     /gene="WASF2"
                     /gene_synonym="dJ393P12.2; IMD2; SCAR2; WASF4; WAVE2"
                     /inference="alignment:Splign:2.1.0"
     exon            894..1049
                     /gene="WASF2"
                     /gene_synonym="dJ393P12.2; IMD2; SCAR2; WASF4; WAVE2"
                     /inference="alignment:Splign:2.1.0"
     exon            1050..1564
                     /gene="WASF2"
                     /gene_synonym="dJ393P12.2; IMD2; SCAR2; WASF4; WAVE2"
                     /inference="alignment:Splign:2.1.0"
     exon            1565..5681
                     /gene="WASF2"
                     /gene_synonym="dJ393P12.2; IMD2; SCAR2; WASF4; WAVE2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5651..5656
                     /regulatory_class="polyA_signal_sequence"
                     /gene="WASF2"
                     /gene_synonym="dJ393P12.2; IMD2; SCAR2; WASF4; WAVE2"
                     /note="hexamer: AATAAT"
     polyA_site      5681
                     /gene="WASF2"
                     /gene_synonym="dJ393P12.2; IMD2; SCAR2; WASF4; WAVE2"
                     /note="major polyA site"
ORIGIN      
        1 gcccagggcg gggaatcgcg taatggcgga cacaggcagg gcgagcgcgg ctgggggcgt
       61 agcgcgctga gggggtccgg ccgtttggca gcccgcgagg cggtccgcgg gagcacactc
      121 tgtgcggaga ctgggcggcc ggccgaccct tcctgtcgct gacggcgact gcgggaggcc
      181 aggttgtttt tcaccattca gaacattgcc tgaagcaggt ccaccatgcc gttagtaacg
      241 aggaacatcg agccaaggca cctgtgccgt cagacgttgc ctagcgttag aagcgagctg
      301 gaatgcgtga ccaacatcac cctggcaaat gtcatccgac agctgggcag cctgagtaaa
      361 tatgcagagg acatttttgg agagctcttt actcaggcaa atacctttgc ctctcgggta
      421 agctcccttg ctgagagggt cgaccgacta caggttaaag tcactcagct ggatcccaag
      481 gaagaagaag tgtcactgca aggaatcaac acccgaaaag ccttcagaag ttccaccatt
      541 caagaccaga agctttttga cagaaactct ctcccagtgc ctgtcttaga aacatacaat
      601 acctgtgata ctcctccccc tctcaacaat cttacccctt acagggacga tggaaaagag
      661 gcactcaaat tctacacaga cccttcatac ttctttgatc tttggaagga gaagatgctg
      721 caggacacca aggatatcat gaaagagaag agaaagcata ggaaagaaaa gaaagataat
      781 ccaaatcgag ggaatgtaaa cccacgtaaa atcaagacac gtaaggaaga gtgggagaaa
      841 atgaagatgg ggcaagaatt tgtggagtcc aaagaaaagc tggggacttc tgggtatcca
      901 cccactttgg tgtaccagaa tggcagcatt ggctgtgttg aaaacgtgga tgcaagtagc
      961 tatccgccac caccacagtc agactctgct tcttcacctt ctccttcctt ctccgaggac
     1021 aacttgcctc ctccaccagc agaattcagt tacccagtgg acaaccaaag aggatctggt
     1081 ttggctggac ccaaaagatc cagtgtggtc agcccaagcc atccaccacc agctcctcct
     1141 ctaggctctc caccaggccc taaacccggg tttgctccac cacctgcccc tccgccacct
     1201 ccgcctccaa tgataggcat cccacctcca ccaccgcctg taggatttgg gtctccaggg
     1261 acgcctccac caccctcacc cccatctttc ccacctcacc ctgattttgc tgcccctcca
     1321 cctcctcctc caccaccagc agctgactac ccaactctgc caccacctcc cttgtcccag
     1381 ccaacaggag gagcacctcc tcctccccct cctcctcctc ctccggggcc ccctcctccc
     1441 cctttcactg gtgcagatgg ccagcctgct ataccaccac cgctttctga taccaccaag
     1501 cccaagtcct ccttgcctgc cgtgagcgat gcccgtagcg acctgctttc agccatccgt
     1561 caaggttttc agctgcgcag ggttgaggag cagcgggaac aagagaagcg ggatgttgtg
     1621 ggcaatgacg tggccaccat cttgtctcgt cgcattgctg ttgagtacag tgactcagaa
     1681 gatgactcct ctgaatttga tgaggacgac tggtccgatt aactctttct gcctgctgcc
     1741 caccttcttt ttctttcctt cctacctgcc ttctttgatg ccaaccccaa cagacccgta
     1801 gggggagaaa agggaggaaa aaagtaattt taaggggcca aagctttccc tgaagcaacc
     1861 aaagatatat ccaagtgctt cctccaagtc aacatgtatt tcctctcccc attttcaggc
     1921 cctgtggggc tcctgaggtt cagtagctgg gatgttccct ctttccttca agtgcctgtt
     1981 gcatattgaa aggaaggaga aatcccaaag cagattcctt tgatcgggtt tctgttggag
     2041 atggggcttc ccttaggagc catattcaac tacagccttc taaaacctgt gccctcagcc
     2101 acttcgaatg ccagccacct tctggttcta aaacggggag tggtctgaat gaacacagct
     2161 gacccctttc ccgcgcactg aaagggcaga gtaggccgaa gggtccaagg gccagactgc
     2221 ctcaccctct gccctaatca gcagggtggg cctgcctttt gctaagcgat ctctatgcct
     2281 gggatgccct ttattccagg aggcatcaag cctctaaaga atgtctcacc tcctctgccc
     2341 aaaaatgatg cctttctgta ggctggtgtt gttgcctccc tcccaggatc cctttggtga
     2401 gtatggtgtt caggatgcac caccaccacc tctagatacc ttcaggcaac acagcccagt
     2461 tttaacctct agtatccatg accaaactat ccctgacaca tgaggacagg ggcctcttct
     2521 ggctgtcagg agcaaagcct gaagacttgg agctgcagga ctggaagaac agtggagccc
     2581 cgtgggtctc accctttaag gatgctgagg cctagagatg ggaagtgact tgctcaaggt
     2641 cacacaattg gatagtgaca tagctagagc gcagagttcc tgattccaag tcacctgtgc
     2701 tttctgggac caaagaatgg gcacctgctg gagtccgggc agagctttct cagttgtatt
     2761 gctactccag acctcaccat aggttggggt cccagtagga aggctcaggg tctgtgccag
     2821 ccctgtcggt gctgctcaga ccttcatagc ctctcttgtc attctttgtt gccccttttc
     2881 tgtcaccagc caaccacata gccttgggac cagcctctct gggggaccag aagtagtgag
     2941 agaaggaagg ggataggcag ctttgacagg tgctgctttc aattcctctg caactcctcc
     3001 cccttttatt tccccaattt aaacaaagat tctgccaact gtggaaactt cagtccctca
     3061 ggctggcagc catgccagta cctgcctggg ggtggggggt gcctggcagc catgaagcag
     3121 gctgaaaggc agaggggctc caggtcctgt ttccagctcc cctcactgca catggtgaag
     3181 ctcgctccct ccctccctcc cttcccgctt ttcccagagc taatacacag gtgctattat
     3241 tcagaaaaaa actggtcagc tctagccaac agtgaggttt cttttcttct gccctaacta
     3301 ttgtgtagcc tcttatgctg aaatcggctt ctgctggctt ctccggcttt cagagccctg
     3361 aaacaaagag aaacaggatc tgtccctacc cagcacagca aatggttgta gtaattgcca
     3421 aagccctcat aaagccctcc ggcttgagga gagagtgtat agtcatgggt tctgcctctg
     3481 tgcccttgct ggccgcttct cctctgcctt ctttcctgga actcagggtg tggggactga
     3541 gcctgtaggg gacagcatgc cgtcttgctg tggccactcc caagtgtgcc ctcttccctc
     3601 tttacacatc aggtgtctct ggcacaggac ttggcactaa gctccatgct gagacaccag
     3661 gctatgtggg cccccacctt gtttcccagc ctgcacctta gaagccgaag gtgctttcat
     3721 cagaacccta aaatggtcgt tgaaggcgcc tgggccgcag cccagcagta gttggagagg
     3781 caggcagagg gcagtggttc tcccaaatag gagacctggg gcctggccag gccagggttt
     3841 gggcctaatg gctttgacta aattaccccc atcctccttg cccggaaaag ggagagctag
     3901 agccactcac tgtcattctg ctctgacctt gaagggggcg gtgttggcct ggcttctgga
     3961 atggactgag tccatcgtgg aaagggctgg gggcaggagg aggtggggag gggcactgcc
     4021 tgcggaaggt aggattagat cattagctca gtgacctcct agggtttcga tgtgctatgt
     4081 tctcatccta cagttggttt ggtaatgatc tgcaagtccc ggagagcaac agcacagctc
     4141 tgcctgacgc tctcattaaa atctatgcag ccaagctcgg cactttgtag cagccggcct
     4201 tgcgaagcct cctcagctcg gggggccggg gacccagtga gccgagaggc cctctgggct
     4261 ccacttatgc atatgcacca aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aggcttcttt
     4321 agacccagag ccctttaaga tgataaagga cccaatgcag gtaatcctgt ttttggactc
     4381 acgtaatgta gaatgcaaag tttagtgatt atttaatctg ggcctaagct agtgagagta
     4441 tggaacaatg gcccataagg actcctaagc gccgaagcca aggagcctcc tcccttcctg
     4501 gttcaccccg cccaagggac ctgcccccat tcctgtggcc ctggggccca gcttccaata
     4561 gtgtcagaag caacttgtgg ctttgccttg gttgtgagat gggtacattc caaaggagca
     4621 gcctggggga gggggcaggc tcagccagcc cttggaggct cagagcagct ggcccaggct
     4681 ccagctcgcc tccagtggaa gcccgagcgc cctcagaaac gaccctggcc aatgtcaacg
     4741 cagagccgag gcggtgttgt cacggctaat gtgaaatctc ttcccgtggc gcccatcctc
     4801 tgactctgca gtgccagctt ggacagattg aggtctctgt ggctttggtg accctgctta
     4861 agcattccca ccgtgtccca gctctgctct aggactgcct cccactgcgg cccctcacct
     4921 gacttcaggg tatggggagg gagggagcca gtgctccaag gccagggggc tttgctcatg
     4981 agagatgtca ggcttcctgg aggcatcagg ctttgagccc tgctggggtc caactgacct
     5041 gggaccaggt gtcgcgcaga gatgtgcctc attctgtgcc tccttctgtg cctcctacca
     5101 tgttcccttt aactgtgcct cagtgtgggt tagggaacaa ttttgttatt gtattaagtt
     5161 tattcagtta attcacttga ggaactaacc agtttttact ttctgtctag aatgatgtac
     5221 atgtagtaga gtaagccata atgctcccaa agtgccttgt tttctctgat acaaacatat
     5281 ttataaggac atattcctgt gggttcttgg gtgtgtttgc cacatcaaga ttttagggaa
     5341 ggggggtata tgtgtagtag ggatgccgtt tgaaaacagg cactcttccc ctgaggatgg
     5401 gaaaggagct cttcagttgg agatctaggc agtgggttaa gccagacccc taaagtgacc
     5461 ctttcctctt ctgccctatt cttctcagac cctcagaaag acatccaatt tttttaaatc
     5521 cctgtagctc caagcctggt ctcatttgct tccctgccag gctcatcgtg tgtataccac
     5581 atttgtgccc cactgacctg aaggttccat gtaactgtat acacatgaat acttaaatgc
     5641 caacatttaa aataatttga taaagtcttt gtagccactc a
//
