LOCUS       NM_003931               2886 bp    mRNA    linear   PRI 03-OCT-2024
DEFINITION  Homo sapiens WASP family member 1 (WASF1), transcript variant 1,
            mRNA.
ACCESSION   NM_003931
VERSION     NM_003931.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2886)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   2  (bases 1 to 2886)
  AUTHORS   Cheng,J., Song,Y., Xu,J., Li,H.H. and Zheng,J.F.
  TITLE     LncRNA PVT1 promotes the malignant progression of acute myeloid
            leukaemia via sponging miR-29 family to increase WAVE1 expression
  JOURNAL   Pathology 53 (5), 613-622 (2021)
   PUBMED   33558065
  REMARK    GeneRIF: LncRNA PVT1 promotes the malignant progression of acute
            myeloid leukaemia via sponging miR-29 family to increase WAVE1
            expression.
REFERENCE   3  (bases 1 to 2886)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   4  (bases 1 to 2886)
  AUTHORS   Ozawa,M., Hiver,S., Yamamoto,T., Shibata,T., Upadhyayula,S.,
            Mimori-Kiyosue,Y. and Takeichi,M.
  TITLE     Adherens junction regulates cryptic lamellipodia formation for
            epithelial cell migration
  JOURNAL   J Cell Biol 219 (10) (2020)
   PUBMED   32886101
  REMARK    GeneRIF: Adherens junction regulates cryptic lamellipodia formation
            for epithelial cell migration.
REFERENCE   5  (bases 1 to 2886)
  AUTHORS   Tang,Q., Schaks,M., Koundinya,N., Yang,C., Pollard,L.W.,
            Svitkina,T.M., Rottner,K. and Goode,B.L.
  TITLE     WAVE1 and WAVE2 have distinct and overlapping roles in controlling
            actin assembly at the leading edge
  JOURNAL   Mol Biol Cell 31 (20), 2168-2178 (2020)
   PUBMED   32697617
  REMARK    GeneRIF: WAVE1 and WAVE2 have distinct and overlapping roles in
            controlling actin assembly at the leading edge.
REFERENCE   6  (bases 1 to 2886)
  AUTHORS   Bear,J.E., Rawls,J.F. and Saxe,C.L. 3rd.
  TITLE     SCAR, a WASP-related protein, isolated as a suppressor of receptor
            defects in late Dictyostelium development
  JOURNAL   J Cell Biol 142 (5), 1325-1335 (1998)
   PUBMED   9732292
REFERENCE   7  (bases 1 to 2886)
  AUTHORS   Witke,W., Podtelejnikov,A.V., Di Nardo,A., Sutherland,J.D.,
            Gurniak,C.B., Dotti,C. and Mann,M.
  TITLE     In mouse brain profilin I and profilin II associate with regulators
            of the endocytic pathway and actin assembly
  JOURNAL   EMBO J 17 (4), 967-976 (1998)
   PUBMED   9463375
REFERENCE   8  (bases 1 to 2886)
  AUTHORS   She,H.Y., Rockow,S., Tang,J., Nishimura,R., Skolnik,E.Y., Chen,M.,
            Margolis,B. and Li,W.
  TITLE     Wiskott-Aldrich syndrome protein is associated with the adapter
            protein Grb2 and the epidermal growth factor receptor in living
            cells
  JOURNAL   Mol Biol Cell 8 (9), 1709-1721 (1997)
   PUBMED   9307968
REFERENCE   9  (bases 1 to 2886)
  AUTHORS   Kitamura,T., Kitamura,Y., Yonezawa,K., Totty,N.F., Gout,I.,
            Hara,K., Waterfield,M.D., Sakaue,M., Ogawa,W. and Kasuga,M.
  TITLE     Molecular cloning of p125Nap1, a protein that associates with an
            SH3 domain of Nck
  JOURNAL   Biochem Biophys Res Commun 219 (2), 509-514 (1996)
   PUBMED   8605018
REFERENCE   10 (bases 1 to 2886)
  AUTHORS   Whitebread,S., Mele,M., Kamber,B. and de Gasparo,M.
  TITLE     Preliminary biochemical characterization of two angiotensin II
            receptor subtypes
  JOURNAL   Biochem Biophys Res Commun 163 (1), 284-291 (1989)
   PUBMED   2775266
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BG719023.1, BI461486.1,
            D87459.1 and AI928895.1.
            
            On Nov 23, 2018 this sequence version replaced NM_003931.2.
            
            Summary: The protein encoded by this gene, a member of the
            Wiskott-Aldrich syndrome protein (WASP)-family, plays a critical
            role downstream of Rac, a Rho-family small GTPase, in regulating
            the actin cytoskeleton required for membrane ruffling. It has been
            shown to associate with an actin nucleation core Arp2/3 complex
            while enhancing actin polymerization in vitro. Wiskott-Aldrich
            syndrome is a disease of the immune system, likely due to defects
            in regulation of actin cytoskeleton. Multiple alternatively spliced
            transcript variants encoding the same protein have been found for
            this gene. [provided by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (1) represents the longest
            transcript.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803611.166743.1,
                                           SRR1660803.26161.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000392589.6/ ENSP00000376368.1
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-130               BG719023.1         428-557
            131-261             BI461486.1         115-245
            262-2546            D87459.1           1-2285
            2547-2886           AI928895.1         11-350              c
FEATURES             Location/Qualifiers
     source          1..2886
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="6"
                     /map="6q21"
     gene            1..2886
                     /gene="WASF1"
                     /gene_synonym="NEDALVS; SCAR1; WAVE; WAVE1"
                     /note="WASP family member 1"
                     /db_xref="GeneID:8936"
                     /db_xref="HGNC:HGNC:12732"
                     /db_xref="MIM:605035"
     exon            1..232
                     /gene="WASF1"
                     /gene_synonym="NEDALVS; SCAR1; WAVE; WAVE1"
                     /inference="alignment:Splign:2.1.0"
     exon            233..377
                     /gene="WASF1"
                     /gene_synonym="NEDALVS; SCAR1; WAVE; WAVE1"
                     /inference="alignment:Splign:2.1.0"
     exon            378..475
                     /gene="WASF1"
                     /gene_synonym="NEDALVS; SCAR1; WAVE; WAVE1"
                     /inference="alignment:Splign:2.1.0"
     exon            476..636
                     /gene="WASF1"
                     /gene_synonym="NEDALVS; SCAR1; WAVE; WAVE1"
                     /inference="alignment:Splign:2.1.0"
     CDS             504..2183
                     /gene="WASF1"
                     /gene_synonym="NEDALVS; SCAR1; WAVE; WAVE1"
                     /note="wiskott-Aldrich syndrome protein family member 1;
                     homology of dictyostelium scar 1; verprolin homology
                     domain-containing protein 1; protein WAVE-1; WAS protein
                     family member 1; WASP family protein member 1"
                     /codon_start=1
                     /product="actin-binding protein WASF1"
                     /protein_id="NP_003922.1"
                     /db_xref="CCDS:CCDS5080.1"
                     /db_xref="GeneID:8936"
                     /db_xref="HGNC:HGNC:12732"
                     /db_xref="MIM:605035"
                     /translation="MPLVKRNIDPRHLCHTALPRGIKNELECVTNISLANIIRQLSSL
                     SKYAEDIFGELFNEAHSFSFRVNSLQERVDRLSVSVTQLDPKEEELSLQDITMRKAFR
                     SSTIQDQQLFDRKTLPIPLQETYDVCEQPPPLNILTPYRDDGKEGLKFYTNPSYFFDL
                     WKEKMLQDTEDKRKEKRKQKQKNLDRPHEPEKVPRAPHDRRREWQKLAQGPELAEDDA
                     NLLHKHIEVANGPASHFETRPQTYVDHMDGSYSLSALPFSQMSELLTRAEERVLVRPH
                     EPPPPPPMHGAGDAKPIPTCISSATGLIENRPQSPATGRTPVFVSPTPPPPPPPLPSA
                     LSTSSLRASMTSTPPPPVPPPPPPPATALQAPAVPPPPAPLQIAPGVLHPAPPPIAPP
                     LVQPSPPVARAAPVCETVPVHPLPQGEVQGLPPPPPPPPLPPPGIRPSSPVTVTALAH
                     PPSGLHPTPSTAPGPHVPLMPPSPPSQVIPASEPKRHPSTLPVISDARSVLLEAIRKG
                     IQLRKVEEQREQEAKHERIENDVATILSRRIAVEYSDSEDDSEFDEVDWLE"
     exon            637..771
                     /gene="WASF1"
                     /gene_synonym="NEDALVS; SCAR1; WAVE; WAVE1"
                     /inference="alignment:Splign:2.1.0"
     exon            772..925
                     /gene="WASF1"
                     /gene_synonym="NEDALVS; SCAR1; WAVE; WAVE1"
                     /inference="alignment:Splign:2.1.0"
     exon            926..1043
                     /gene="WASF1"
                     /gene_synonym="NEDALVS; SCAR1; WAVE; WAVE1"
                     /inference="alignment:Splign:2.1.0"
     exon            1044..1216
                     /gene="WASF1"
                     /gene_synonym="NEDALVS; SCAR1; WAVE; WAVE1"
                     /inference="alignment:Splign:2.1.0"
     exon            1217..1396
                     /gene="WASF1"
                     /gene_synonym="NEDALVS; SCAR1; WAVE; WAVE1"
                     /inference="alignment:Splign:2.1.0"
     exon            1397..2025
                     /gene="WASF1"
                     /gene_synonym="NEDALVS; SCAR1; WAVE; WAVE1"
                     /inference="alignment:Splign:2.1.0"
     exon            2026..2886
                     /gene="WASF1"
                     /gene_synonym="NEDALVS; SCAR1; WAVE; WAVE1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2863..2868
                     /regulatory_class="polyA_signal_sequence"
                     /gene="WASF1"
                     /gene_synonym="NEDALVS; SCAR1; WAVE; WAVE1"
                     /note="hexamer: AATAAA"
     polyA_site      2886
                     /gene="WASF1"
                     /gene_synonym="NEDALVS; SCAR1; WAVE; WAVE1"
                     /note="major polyA site"
ORIGIN      
        1 agctcgcggt gttgttcact cgcgcgtcga gcacacggtg ggtccggcgg cgggttggcg
       61 ccccaggcgg cgttccctgt ggcctggcgc ctgggccgct gccctgagcg ggttccgccc
      121 cagagcccga ccctcctggg ggctctaggc ggagtcccgc gagccgaggg ggaccggcga
      181 ccgctgccga agcatgaaga aggggtaagg cgtgagcccc caagatttca cgcatgcccc
      241 ctagcttcgg tactctgaca ccttctcttg cacttgcgga tgatgaactg gaataacgat
      301 gaaagaaagc acatccgatc tcaacattca cgtcctgccc tataaccgat taattaattg
      361 atccccagct agactagtgt tggagaaatc agcatgttaa aacaactgtt gatgatagct
      421 gttggagtaa agttgcagtg gaagctatgg ctgcaaaatc gttaaaatct tcaaggtgaa
      481 ctggcacaaa ggttaatctc aagatgccgc tagtgaaaag aaacatcgat cctaggcact
      541 tgtgccacac agcactgcct agaggcatta agaatgaact ggaatgtgta accaatattt
      601 ccttggcaaa tataattaga caactaagta gcctaagtaa atatgctgaa gatatatttg
      661 gagaattatt caatgaagca catagttttt ccttcagagt caactcattg caagaacgtg
      721 tggaccgttt atctgttagt gttacacagc ttgatccaaa ggaagaagaa ttgtctttgc
      781 aagatataac aatgaggaaa gctttccgaa gttctacaat tcaagaccag cagcttttcg
      841 atcgcaagac tttgcctatt ccattacagg agacgtacga tgtttgtgaa cagcctccac
      901 ctctcaatat actcactcct tatagagatg atggtaaaga aggtctgaag ttttatacca
      961 atccttcgta tttctttgat ctatggaaag aaaaaatgtt gcaagataca gaggataaga
     1021 ggaaggaaaa gaggaagcag aagcagaaaa atctagatcg tcctcatgaa ccagaaaaag
     1081 tgccaagagc acctcatgac aggcggcgag aatggcagaa gctggcccaa ggtccagagc
     1141 tggctgaaga tgatgctaat ctcttacata agcatattga agttgctaat ggcccagcct
     1201 ctcattttga aacaagacct cagacatacg tggatcatat ggatggatct tactcacttt
     1261 ctgccttgcc atttagtcag atgagtgagc ttctgactag agctgaggaa agggtattag
     1321 tcagaccaca tgaaccacct ccacctccac caatgcatgg agcaggagat gcaaaaccga
     1381 tacccacctg tatcagttct gctacaggtt tgatagaaaa tcgccctcag tcaccagcta
     1441 caggcagaac acctgtgttt gtgagcccca ctcccccacc tcctccacca cctcttccat
     1501 ctgccttgtc aacttcctca ttaagagctt caatgacttc aactcctccc cctccagtac
     1561 ctcccccacc tccacctcca gccactgctt tgcaagctcc agcagtacca ccacctccag
     1621 ctcctcttca gattgcccct ggagttcttc acccagctcc tcctccaatt gcacctcctc
     1681 tagtacagcc ctctccacca gtagctagag ctgccccagt atgtgagact gtaccagttc
     1741 atccactccc acaaggtgaa gttcaggggc tgcctccacc cccaccaccg cctcctctgc
     1801 ctccacctgg cattcgacca tcatcacctg tcacagttac agctcttgct catcctccct
     1861 ctgggctaca tccaactcca tctactgccc caggtcccca tgttccatta atgcctccat
     1921 ctcctccatc acaagttata cctgcttctg agccaaagcg ccatccatca accctacctg
     1981 taatcagtga tgccaggagt gtgctactgg aagcaatacg aaaaggtatt cagctacgca
     2041 aagtagaaga gcagcgtgaa caggaagcta agcatgaacg cattgaaaac gatgttgcca
     2101 ccatcctgtc tcgccgtatt gctgttgaat atagtgattc ggaagatgat tcagaatttg
     2161 atgaagtaga ttggttggag taagaaaaat gcattgataa atattacaaa actgaatgca
     2221 aatgtccttt gtggtgcttg ttccttgaaa atgtttggtc attctagtgt tttgctttct
     2281 tttccttata ataaatgacc cttttcctcc ataacttttg atttctaagg aaaatattag
     2341 catacatttc aaactaaatg ttttacagtg gcttatcttt tttttccccc tgaaaagact
     2401 aatttggtca aataaaccac taagtattaa gcatggacag ctgttgttag agtagcagat
     2461 tcagtttttt gatatatctt aattgtgtac tttgtgaatt ttaatttaaa gaaagcaact
     2521 gaaattgaaa tcttgagggc agctgtgtct actaatgagc cttattccat ttcctgatgt
     2581 tttaaaagaa gaaacactgc cttgattata cgaatacact cagaaagtac atttagcttg
     2641 tagtgttgaa ttctcttaaa ggaatgcttg aattttttca ttattgtttt attgttttta
     2701 tatacttgcc ttatttgaat gtttagcagt atccccttcc cacttatata ttgtgtgata
     2761 tgattttgct tgcctatagg agttaaaaac ttttccatgt gaaatactct gacttaaaca
     2821 tacatgtaac ttacataact gttaagaata acagtctgat ttaataaatg gttcatttta
     2881 aaagtt
//
