LOCUS       NM_006296               1773 bp    mRNA    linear   PRI 07-APR-2024
DEFINITION  Homo sapiens VRK serine/threonine kinase 2 (VRK2), transcript
            variant 1, mRNA.
ACCESSION   NM_006296
VERSION     NM_006296.7
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1773)
  AUTHORS   Puja,R., Dutta,S. and Bose,K.
  TITLE     Elucidating the interaction of C-terminal domain of
            Vaccinia-Related Kinase 2A (VRK2A) with B-cell lymphoma-extra Large
            (Bcl-xL) to decipher its anti-apoptotic role in cancer
  JOURNAL   Biochem J 480 (22), 1871-1885 (2023)
   PUBMED   37943248
  REMARK    GeneRIF: Elucidating the interaction of C-terminal domain of
            Vaccinia-Related Kinase 2A (VRK2A) with B-cell lymphoma-extra Large
            (Bcl-xL) to decipher its anti-apoptotic role in cancer.
REFERENCE   2  (bases 1 to 1773)
  AUTHORS   Yin,M.Y., Guo,L., Zhao,L.J., Zhang,C., Liu,W.P., Zhang,C.Y.,
            Huo,J.H., Wang,L., Li,S.W., Zheng,C.B., Xiao,X., Li,M., Wang,C. and
            Chang,H.
  TITLE     Reduced Vrk2 expression is associated with higher risk of
            depression in humans and mediates depressive-like behaviors in mice
  JOURNAL   BMC Med 21 (1), 256 (2023)
   PUBMED   37452335
  REMARK    GeneRIF: Reduced Vrk2 expression is associated with higher risk of
            depression in humans and mediates depressive-like behaviors in
            mice.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1773)
  AUTHORS   So,J., Mabe,N.W., Englinger,B., Chow,K.H., Moyer,S.M., Yerrum,S.,
            Trissal,M.C., Marques,J.G., Kwon,J.J., Shim,B., Pal,S.,
            Panditharatna,E., Quinn,T., Schaefer,D.A., Jeong,D., Mayhew,D.L.,
            Hwang,J., Beroukhim,R., Ligon,K.L., Stegmaier,K., Filbin,M.G. and
            Hahn,W.C.
  TITLE     VRK1 as a synthetic lethal target in VRK2 promoter-methylated
            cancers of the nervous system
  JOURNAL   JCI Insight 7 (19), e158755 (2022)
   PUBMED   36040810
  REMARK    GeneRIF: VRK1 as a synthetic lethal target in VRK2
            promoter-methylated cancers of the nervous system.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1773)
  AUTHORS   Chen,J., Qiao,K., Zhang,C., Zhou,X., Du,Q., Deng,Y. and Cao,L.
  TITLE     VRK2 activates TNFalpha/NF-kappaB signaling by phosphorylating
            IKKbeta in pancreatic cancer
  JOURNAL   Int J Biol Sci 18 (3), 1288-1302 (2022)
   PUBMED   35173553
  REMARK    GeneRIF: VRK2 activates TNFalpha/NF-kappaB signaling by
            phosphorylating IKKbeta in pancreatic cancer.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 1773)
  AUTHORS   Blanco,S., Santos,C. and Lazo,P.A.
  TITLE     Vaccinia-related kinase 2 modulates the stress response to hypoxia
            mediated by TAK1
  JOURNAL   Mol Cell Biol 27 (20), 7273-7283 (2007)
   PUBMED   17709393
  REMARK    GeneRIF: Data show that TAK1 forms a stable complex with JIP1 and
            regulates the activation of JNK, which in turn determines the
            cellular stress response to hypoxia; this activation of
            TAK1-JIP1-JNK is suppressed by vaccinia-related kinase 2.
REFERENCE   6  (bases 1 to 1773)
  AUTHORS   Li,L.Y., Liu,M.Y., Shih,H.M., Tsai,C.H. and Chen,J.Y.
  TITLE     Human cellular protein VRK2 interacts specifically with
            Epstein-Barr virus BHRF1, a homologue of Bcl-2, and enhances cell
            survival
  JOURNAL   J Gen Virol 87 (Pt 10), 2869-2878 (2006)
   PUBMED   16963744
  REMARK    GeneRIF: human VRK2 interacts specifically with EBV BHRF1 and that
            the interaction is involved in protecting cells from apoptosis
REFERENCE   7  (bases 1 to 1773)
  AUTHORS   Blanco,S., Klimcakova,L., Vega,F.M. and Lazo,P.A.
  TITLE     The subcellular localization of vaccinia-related kinase-2 (VRK2)
            isoforms determines their different effect on p53 stability in
            tumour cell lines
  JOURNAL   FEBS J 273 (11), 2487-2504 (2006)
   PUBMED   16704422
  REMARK    GeneRIF: VRK2 induces p53 stabilization by post-translational
            modification, largely due to threonine 18 phosphorylation in tumor
            cell lines.
REFERENCE   8  (bases 1 to 1773)
  AUTHORS   Nichols,R.J., Wiebe,M.S. and Traktman,P.
  TITLE     The vaccinia-related kinases phosphorylate the N' terminus of BAF,
            regulating its interaction with DNA and its retention in the
            nucleus
  JOURNAL   Mol Biol Cell 17 (5), 2451-2464 (2006)
   PUBMED   16495336
REFERENCE   9  (bases 1 to 1773)
  AUTHORS   Nichols,R.J. and Traktman,P.
  TITLE     Characterization of three paralogous members of the Mammalian
            vaccinia related kinase family
  JOURNAL   J Biol Chem 279 (9), 7934-7946 (2004)
   PUBMED   14645249
REFERENCE   10 (bases 1 to 1773)
  AUTHORS   Nezu,J., Oku,A., Jones,M.H. and Shimane,M.
  TITLE     Identification of two novel human putative serine/threonine
            kinases, VRK1 and VRK2, with structural similarity to vaccinia
            virus B1R kinase
  JOURNAL   Genomics 45 (2), 327-331 (1997)
   PUBMED   9344656
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BC027854.1 and AK223540.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Feb 27, 2020 this sequence version replaced NM_006296.6.
            
            Summary: This gene encodes a member of the vaccinia-related kinase
            (VRK) family of serine/threonine protein kinases. The encoded
            protein acts as an effector of signaling pathways that regulate
            apoptosis and tumor cell growth. Variants in this gene have been
            associated with schizophrenia. Alternative splicing results in
            multiple transcript variants that differ in their subcellular
            localization and biological activity. [provided by RefSeq, Jan
            2014].
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (1). Variants 1, 2, 3, and 8 encode the same isoform (1, also known
            as VRK2A).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC027854.1, AK058199.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000340157.9/ ENSP00000342381.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-566               BC027854.1         116-681
            567-567             AK223540.1         586-586
            568-1773            BC027854.1         683-1888
FEATURES             Location/Qualifiers
     source          1..1773
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2p16.1"
     gene            1..1773
                     /gene="VRK2"
                     /note="VRK serine/threonine kinase 2"
                     /db_xref="GeneID:7444"
                     /db_xref="HGNC:HGNC:12719"
                     /db_xref="MIM:602169"
     exon            1..63
                     /gene="VRK2"
                     /inference="alignment:Splign:2.1.0"
     exon            64..204
                     /gene="VRK2"
                     /inference="alignment:Splign:2.1.0"
     CDS             69..1595
                     /gene="VRK2"
                     /EC_number="2.7.11.1"
                     /note="isoform 1 is encoded by transcript variant 1;
                     serine/threonine-protein kinase VRK2; vaccinia virus
                     B1R-related kinase 2; vaccinia related kinase 2"
                     /codon_start=1
                     /product="serine/threonine-protein kinase VRK2 isoform 1"
                     /protein_id="NP_006287.2"
                     /db_xref="CCDS:CCDS1859.1"
                     /db_xref="GeneID:7444"
                     /db_xref="HGNC:HGNC:12719"
                     /db_xref="MIM:602169"
                     /translation="MPPKRNEKYKLPIPFPEGKVLDDMEGNQWVLGKKIGSGGFGLIY
                     LAFPTNKPEKDARHVVKVEYQENGPLFSELKFYQRVAKKDCIKKWIERKQLDYLGIPL
                     FYGSGLTEFKGRSYRFMVMERLGIDLQKISGQNGTFKKSTVLQLGIRMLDVLEYIHEN
                     EYVHGDIKAANLLLGYKNPDQVYLADYGLSYRYCPNGNHKQYQENPRKGHNGTIEFTS
                     LDAHKGVALSRRSDVEILGYCMLRWLCGKLPWEQNLKDPVAVQTAKTNLLDELPQSVL
                     KWAPSGSSCCEIAQFLVCAHSLAYDEKPNYQALKKILNPHGIPLGPLDFSTKGQSINV
                     HTPNSQKVDSQKAATKQVNKAHNRLIEKKVHSERSAESCATWKVQKEEKLIGLMNNEA
                     AQESTRRRQKYQESQEPLNEVNSFPQKISYTQFPNSFYEPHQDFTSPDIFKKSRSPSW
                     YKYTSTVSTGITDLESSTGLWPTISQFTLSEETNADVYYYRIIIPVLLMLVFLALFFL
                     "
     exon            205..254
                     /gene="VRK2"
                     /inference="alignment:Splign:2.1.0"
     exon            255..324
                     /gene="VRK2"
                     /inference="alignment:Splign:2.1.0"
     exon            325..412
                     /gene="VRK2"
                     /inference="alignment:Splign:2.1.0"
     exon            413..518
                     /gene="VRK2"
                     /inference="alignment:Splign:2.1.0"
     exon            519..611
                     /gene="VRK2"
                     /inference="alignment:Splign:2.1.0"
     exon            612..744
                     /gene="VRK2"
                     /inference="alignment:Splign:2.1.0"
     exon            745..865
                     /gene="VRK2"
                     /inference="alignment:Splign:2.1.0"
     exon            866..924
                     /gene="VRK2"
                     /inference="alignment:Splign:2.1.0"
     exon            925..1091
                     /gene="VRK2"
                     /inference="alignment:Splign:2.1.0"
     exon            1092..1250
                     /gene="VRK2"
                     /inference="alignment:Splign:2.1.0"
     exon            1251..1773
                     /gene="VRK2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1750..1755
                     /regulatory_class="polyA_signal_sequence"
                     /gene="VRK2"
                     /note="hexamer: AATAAA"
     polyA_site      1773
                     /gene="VRK2"
                     /note="major polyA site"
ORIGIN      
        1 ctcgtcgcag cggcaggtag gaggcggtgc gcgcggcccg gcgacggggg atcctgaggc
       61 ccgaagtgat gccaccaaaa agaaatgaaa aatacaaact tcctattcca tttccagaag
      121 gcaaggttct ggatgatatg gaaggcaatc agtgggtact gggcaagaag attggctctg
      181 gaggatttgg attgatatat ttagctttcc ccacaaataa accagagaaa gatgcaagac
      241 atgtagtaaa agtggaatat caagaaaatg gcccgttatt ttcagaactt aaattttatc
      301 agagagttgc aaaaaaagac tgtatcaaaa agtggataga acgcaaacaa cttgattatt
      361 taggaattcc tctgttttat ggatctggtc tgactgaatt caagggaaga agttacagat
      421 ttatggtaat ggaaagacta ggaatagatt tacagaagat ctcaggccag aatggtacct
      481 ttaaaaagtc aactgtcctg caattaggta tccgaatgtt ggatgtactg gaatatatac
      541 atgaaaatga atatgttcat ggtgatataa aagcagcaaa tctacttttg ggttacaaaa
      601 atccagacca ggtttatctt gcagattatg gactttccta cagatattgt cccaatggga
      661 accacaaaca gtatcaggaa aatcctagaa aaggccataa tgggacaata gagtttacca
      721 gcttggatgc ccacaaggga gtagccttgt ccagacgaag tgacgttgag atcctcggct
      781 actgcatgct gcggtggttg tgtgggaaac ttccctggga acagaacctg aaggaccctg
      841 tggctgtgca gactgctaaa acaaatctgt tggacgagct cccccagtca gtgcttaaat
      901 gggctccttc tggaagcagt tgctgtgaaa tagcccaatt tttggtatgt gctcatagtt
      961 tagcatatga tgaaaagcca aactatcaag ccctcaagaa aattttgaac cctcatggaa
     1021 tacctttagg accactggac ttttccacaa aaggacagag tataaatgtc catactccaa
     1081 acagtcaaaa agttgattca caaaaggctg caacaaagca agtcaacaag gcacacaata
     1141 ggttaatcga aaaaaaagtc cacagtgaga gaagcgctga gtcctgtgca acatggaaag
     1201 tgcagaaaga ggagaaactg attggattga tgaacaatga agcagctcag gaaagcacaa
     1261 ggagaagaca gaaatatcaa gagtctcaag aacctttgaa tgaagtaaac agtttcccac
     1321 aaaaaatcag ctatacacaa ttcccaaact cattttatga gcctcatcaa gattttacca
     1381 gtccagatat attcaagaag tcaagatctc catcttggta taaatacact tccacagtca
     1441 gcacggggat cacagactta gaaagttcaa ctggactttg gcctacaatt tcccagttta
     1501 ctcttagtga agagacaaac gcagatgttt attattatcg catcatcata cctgtccttt
     1561 tgatgttagt atttcttgct ttattttttc tctgaagatg ataccaaaat tccttttgat
     1621 aattttttaa gtttccagct cttcaccgaa atgttgtatt cttatttcag tgtttccttc
     1681 cagacatttt taaggtaatt ggctttaaaa agagaacata ttttaacaaa gtttgtggac
     1741 actctaaaaa ataaaattgc tttgtactag aaa
//
