LOCUS       NM_003384               1663 bp    mRNA    linear   PRI 09-JUL-2024
DEFINITION  Homo sapiens VRK serine/threonine kinase 1 (VRK1), transcript
            variant 1, mRNA.
ACCESSION   NM_003384
VERSION     NM_003384.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1663)
  AUTHORS   Oliveira,D., Assoni,A.F., Alves,L.M., Sakugawa,A., Melo,U.S., Teles
            E Silva,A.L., Sertie,A.L., Caires,L.C., Goulart,E., Ghirotto,B.,
            Carvalho,V.M., Ferrari,M.R. and Zatz,M.
  TITLE     ALS-associated VRK1 R321C mutation causes proteostatic imbalance
            and mitochondrial defects in iPSC-derived motor neurons
  JOURNAL   Neurobiol Dis 198, 106540 (2024)
   PUBMED   38806131
  REMARK    GeneRIF: ALS-associated VRK1 R321C mutation causes proteostatic
            imbalance and mitochondrial defects in iPSC-derived motor neurons.
REFERENCE   2  (bases 1 to 1663)
  AUTHORS   Navarro-Carrasco,E., Monte-Serrano,E., Campos-Diaz,A., Rolfs,F., de
            Goeij-de Haas,R., Pham,T.V., Piersma,S.R., Gonzalez-Alonso,P.,
            Jimenez,C.R. and Lazo,P.A.
  TITLE     VRK1 Regulates Sensitivity to Oxidative Stress by Altering Histone
            Epigenetic Modifications and the Nuclear Phosphoproteome in Tumor
            Cells
  JOURNAL   Int J Mol Sci 25 (9), 4874 (2024)
   PUBMED   38732093
  REMARK    GeneRIF: VRK1 Regulates Sensitivity to Oxidative Stress by Altering
            Histone Epigenetic Modifications and the Nuclear Phosphoproteome in
            Tumor Cells.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1663)
  AUTHORS   Fan,Z., Wang,X., Cheng,H. and Pan,M.
  TITLE     VRK1 promotes DNA-induced type I interferon production
  JOURNAL   Mol Biol Rep 51 (1), 453 (2024)
   PUBMED   38536553
  REMARK    GeneRIF: VRK1 promotes DNA-induced type I interferon production.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1663)
  AUTHORS   Navarro-Carrasco,E., Campos-Diaz,A., Monte-Serrano,E., Rolfs,F., de
            Goeij-de Haas,R., Pham,T.V., Piersma,S.R., Jimenez,C.R. and
            Lazo,P.A.
  TITLE     Loss of VRK1 alters the nuclear phosphoproteome in the DNA damage
            response to doxorubicin
  JOURNAL   Chem Biol Interact 391, 110908 (2024)
   PUBMED   38367682
  REMARK    GeneRIF: Loss of VRK1 alters the nuclear phosphoproteome in the DNA
            damage response to doxorubicin.
REFERENCE   5  (bases 1 to 1663)
  AUTHORS   Du,N., Zhang,B. and Zhang,Y.
  TITLE     Downregulation of VRK1 Inhibits Progression of Lung Squamous Cell
            Carcinoma through DNA Damage
  JOURNAL   Can Respir J 2023, 4533504 (2023)
   PUBMED   37547297
  REMARK    GeneRIF: Downregulation of VRK1 Inhibits Progression of Lung
            Squamous Cell Carcinoma through DNA Damage.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 1663)
  AUTHORS   Latonen,L., Taya,Y. and Laiho,M.
  TITLE     UV-radiation induces dose-dependent regulation of p53 response and
            modulates p53-HDM2 interaction in human fibroblasts
  JOURNAL   Oncogene 20 (46), 6784-6793 (2001)
   PUBMED   11709713
  REMARK    Erratum:[Oncogene 2001 Dec 6;20(56):8165]
REFERENCE   7  (bases 1 to 1663)
  AUTHORS   Lopez-Borges,S. and Lazo,P.A.
  TITLE     The human vaccinia-related kinase 1 (VRK1) phosphorylates
            threonine-18 within the mdm-2 binding site of the p53 tumour
            suppressor protein
  JOURNAL   Oncogene 19 (32), 3656-3664 (2000)
   PUBMED   10951572
REFERENCE   8  (bases 1 to 1663)
  AUTHORS   Sayed,M., Kim,S.O., Salh,B.S., Issinger,O.G. and Pelech,S.L.
  TITLE     Stress-induced activation of protein kinase CK2 by direct
            interaction with p38 mitogen-activated protein kinase
  JOURNAL   J Biol Chem 275 (22), 16569-16573 (2000)
   PUBMED   10747897
REFERENCE   9  (bases 1 to 1663)
  AUTHORS   Sugimoto,J., Yamauchi,T., Hatakeyama,T. and Isobe,M.
  TITLE     Isolation and mapping of a polymorphic CA repeat sequence at the
            human VRK1 locus
  JOURNAL   J Hum Genet 44 (2), 133-134 (1999)
   PUBMED   10083742
REFERENCE   10 (bases 1 to 1663)
  AUTHORS   Nezu,J., Oku,A., Jones,M.H. and Shimane,M.
  TITLE     Identification of two novel human putative serine/threonine
            kinases, VRK1 and VRK2, with structural similarity to vaccinia
            virus B1R kinase
  JOURNAL   Genomics 45 (2), 327-331 (1997)
   PUBMED   9344656
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BU535261.1, AL133168.6 and
            CA436761.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_003384.2.
            
            Summary: This gene encodes a member of the vaccinia-related kinase
            (VRK) family of serine/threonine protein kinases. This gene is
            widely expressed in human tissues and has increased expression in
            actively dividing cells, such as those in testis, thymus, fetal
            liver, and carcinomas. Its protein localizes to the nucleus and has
            been shown to promote the stability and nuclear accumulation of a
            transcriptionally active p53 molecule and, in vitro, to
            phosphorylate Thr18 of p53 and reduce p53 ubiquitination. This
            gene, therefore, may regulate cell proliferation. This protein also
            phosphorylates histone, casein, and the transcription factors ATF2
            (activating transcription factor 2) and c-JUN. [provided by RefSeq,
            Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC103761.1, SRR1803611.253760.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000216639.8/ ENSP00000216639.3
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-23                BU535261.1         56-78
            24-66               AL133168.6         164921-164963       c
            67-231              AL133168.6         128737-128901       c
            232-287             AL133168.6         124551-124606       c
            288-357             AL133168.6         116204-116273       c
            358-445             AL133168.6         115024-115111       c
            446-554             AL133168.6         109429-109537       c
            555-647             AL133168.6         109202-109294       c
            648-780             AL133168.6         107012-107144       c
            781-901             AL133168.6         106118-106238       c
            902-960             AL133168.6         105782-105840       c
            961-1139            AL133168.6         101633-101811       c
            1140-1230           AL133168.6         86248-86338         c
            1231-1657           AL133168.6         80765-81191         c
            1658-1663           CA436761.1         23-28               c
FEATURES             Location/Qualifiers
     source          1..1663
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="14"
                     /map="14q32.2"
     gene            1..1663
                     /gene="VRK1"
                     /gene_synonym="HMNR10; PCH1; PCH1A"
                     /note="VRK serine/threonine kinase 1"
                     /db_xref="GeneID:7443"
                     /db_xref="HGNC:HGNC:12718"
                     /db_xref="MIM:602168"
     exon            1..66
                     /gene="VRK1"
                     /gene_synonym="HMNR10; PCH1; PCH1A"
                     /inference="alignment:Splign:2.1.0"
     exon            67..231
                     /gene="VRK1"
                     /gene_synonym="HMNR10; PCH1; PCH1A"
                     /inference="alignment:Splign:2.1.0"
     CDS             72..1262
                     /gene="VRK1"
                     /gene_synonym="HMNR10; PCH1; PCH1A"
                     /EC_number="2.7.11.1"
                     /note="isoform 1 is encoded by transcript variant 1;
                     vaccinia virus B1R-related kinase 1;
                     serine/threonine-protein kinase VRK1; vaccinia related
                     kinase 1"
                     /codon_start=1
                     /product="serine/threonine-protein kinase VRK1 isoform 1"
                     /protein_id="NP_003375.1"
                     /db_xref="CCDS:CCDS9947.1"
                     /db_xref="GeneID:7443"
                     /db_xref="HGNC:HGNC:12718"
                     /db_xref="MIM:602168"
                     /translation="MPRVKAAQAGRQSSAKRHLAEQFAVGEIITDMAKKEWKVGLPIG
                     QGGFGCIYLADMNSSESVGSDAPCVVKVEPSDNGPLFTELKFYQRAAKPEQIQKWIRT
                     RKLKYLGVPKYWGSGLHDKNGKSYRFMIMDRFGSDLQKIYEANAKRFSRKTVLQLSLR
                     ILDILEYIHEHEYVHGDIKASNLLLNYKNPDQVYLVDYGLAYRYCPEGVHKEYKEDPK
                     RCHDGTIEFTSIDAHNGVAPSRRGDLEILGYCMIQWLTGHLPWEDNLKDPKYVRDSKI
                     RYRENIASLMDKCFPEKNKPGEIAKYMETVKLLDYTEKPLYENLRDILLQGLKAIGSK
                     DDGKLDLSVVENGGLKAKTITKKRKKEIEESKEPGVEDTEWSNTQTEEAIQTRSRTRK
                     RVQK"
     exon            232..287
                     /gene="VRK1"
                     /gene_synonym="HMNR10; PCH1; PCH1A"
                     /inference="alignment:Splign:2.1.0"
     exon            288..357
                     /gene="VRK1"
                     /gene_synonym="HMNR10; PCH1; PCH1A"
                     /inference="alignment:Splign:2.1.0"
     exon            358..445
                     /gene="VRK1"
                     /gene_synonym="HMNR10; PCH1; PCH1A"
                     /inference="alignment:Splign:2.1.0"
     exon            446..554
                     /gene="VRK1"
                     /gene_synonym="HMNR10; PCH1; PCH1A"
                     /inference="alignment:Splign:2.1.0"
     exon            555..647
                     /gene="VRK1"
                     /gene_synonym="HMNR10; PCH1; PCH1A"
                     /inference="alignment:Splign:2.1.0"
     exon            648..780
                     /gene="VRK1"
                     /gene_synonym="HMNR10; PCH1; PCH1A"
                     /inference="alignment:Splign:2.1.0"
     exon            781..901
                     /gene="VRK1"
                     /gene_synonym="HMNR10; PCH1; PCH1A"
                     /inference="alignment:Splign:2.1.0"
     exon            902..960
                     /gene="VRK1"
                     /gene_synonym="HMNR10; PCH1; PCH1A"
                     /inference="alignment:Splign:2.1.0"
     exon            961..1139
                     /gene="VRK1"
                     /gene_synonym="HMNR10; PCH1; PCH1A"
                     /inference="alignment:Splign:2.1.0"
     exon            1140..1230
                     /gene="VRK1"
                     /gene_synonym="HMNR10; PCH1; PCH1A"
                     /inference="alignment:Splign:2.1.0"
     exon            1231..1663
                     /gene="VRK1"
                     /gene_synonym="HMNR10; PCH1; PCH1A"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1434..1439
                     /regulatory_class="polyA_signal_sequence"
                     /gene="VRK1"
                     /gene_synonym="HMNR10; PCH1; PCH1A"
                     /note="hexamer: ATTAAA"
     polyA_site      1462
                     /gene="VRK1"
                     /gene_synonym="HMNR10; PCH1; PCH1A"
                     /note="major polyA site"
     regulatory      1632..1637
                     /regulatory_class="polyA_signal_sequence"
                     /gene="VRK1"
                     /gene_synonym="HMNR10; PCH1; PCH1A"
                     /note="hexamer: AATAAA"
     polyA_site      1663
                     /gene="VRK1"
                     /gene_synonym="HMNR10; PCH1; PCH1A"
                     /note="major polyA site"
ORIGIN      
        1 gttacgagtc ggcgaaagcg gcgggaagtt cgtactgggc agaacgcgac gggtctgcgg
       61 cttaggtgaa aatgcctcgt gtaaaagcag ctcaagctgg aagacagagc tctgcaaaga
      121 gacatcttgc agaacaattt gcagttggag agataataac tgacatggca aaaaaggaat
      181 ggaaagtagg attacccatt ggccaaggag gctttggctg tatatatctt gctgatatga
      241 attcttcaga gtcagttggc agtgatgcac cttgtgttgt aaaagtggaa cccagtgaca
      301 atggacctct ttttactgaa ttaaagttct accaacgagc tgcaaaacca gagcaaattc
      361 agaaatggat tcgtacccgt aagctgaagt acctgggtgt tcctaagtat tgggggtctg
      421 gtctacatga caaaaatgga aaaagttaca ggtttatgat aatggatcgc tttgggagtg
      481 accttcagaa aatatatgaa gcaaatgcca aaaggttttc tcggaaaact gtcttgcagc
      541 taagcttaag aattctggat attctggaat atattcacga gcatgagtat gtgcatggag
      601 atatcaaggc ctcaaatctt cttctgaact acaagaatcc tgaccaggtg tacttggtag
      661 attatggcct tgcttatcgg tactgcccag aaggagttca taaagaatac aaagaagacc
      721 ccaaaagatg tcacgatggc actattgaat tcacgagcat cgatgcacac aatggcgtgg
      781 ccccatcaag acgtggtgat ttggaaatac ttggttattg catgatccaa tggcttactg
      841 gccatcttcc ttgggaggat aatttgaaag atcctaaata tgttagagat tccaaaatta
      901 gatacagaga aaatattgca agtttgatgg acaaatgttt tcctgagaaa aacaaaccag
      961 gtgaaattgc caaatacatg gaaacagtga aattactaga ctacactgaa aaacctcttt
     1021 atgaaaattt acgtgacatt cttttgcaag gactaaaagc tataggaagt aaggatgatg
     1081 gcaaattgga cctcagtgtt gtggagaatg gaggtttgaa agcaaaaaca ataacaaaga
     1141 agcgaaagaa agaaattgaa gaaagcaagg aacctggtgt tgaagatacg gaatggtcaa
     1201 acacacagac agaggaggcc atacagaccc gttcaagaac cagaaagaga gtccagaagt
     1261 aattcagatg ctgtgaacca gatttccttt tctttgtttt cttttgactt ttttctcctt
     1321 ttctatttga actgttttat tttcctgtga gtcttgcgag gtggaagtaa tgattaaata
     1381 ctcatgtgtt cagaaaacat aaactttttt tataaaaata ttttgtacaa ttcattaaag
     1441 gctaatttat gaaatttgaa aatcttcagg ttatactcct taagttatcc caaagccgtg
     1501 tgtttgtgat gttttggagt acatatatat gaaaattatt atgacacgca cttttctaat
     1561 cattgtacat ttctcagagt ggataaaaat gtttgacaaa gtcctcactt ttaaggaaat
     1621 gcaaagctta aaataaaact ctcttttgtt tgatgcaaac aca
//
