LOCUS       NM_001009921            5014 bp    mRNA    linear   PRI 12-FEB-2025
DEFINITION  Homo sapiens VPS8 subunit of CORVET complex (VPS8), transcript
            variant 1, mRNA.
ACCESSION   NM_001009921
VERSION     NM_001009921.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5014)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   2  (bases 1 to 5014)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   3  (bases 1 to 5014)
  AUTHORS   van der Beek,J., Jonker,C., van der Welle,R., Liv,N. and
            Klumperman,J.
  TITLE     CORVET, CHEVI and HOPS - multisubunit tethers of the endo-lysosomal
            system in health and disease
  JOURNAL   J Cell Sci 132 (10) (2019)
   PUBMED   31092635
  REMARK    Review article
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 5014)
  AUTHORS   Jonker,C.T.H., Galmes,R., Veenendaal,T., Ten Brink,C., van der
            Welle,R.E.N., Liv,N., de Rooij,J., Peden,A.A., van der Sluijs,P.,
            Margadant,C. and Klumperman,J.
  TITLE     Vps3 and Vps8 control integrin trafficking from early to recycling
            endosomes and regulate integrin-dependent functions
  JOURNAL   Nat Commun 9 (1), 792 (2018)
   PUBMED   29476049
  REMARK    GeneRIF: Vps3 and Vps8 are required for recycling of beta1
            integrins.
            Erratum:[Nat Commun. 2021 Sep 29;12(1):5828. doi:
            10.1038/s41467-021-25201-6.. PMID: 34588435]
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 5014)
  AUTHORS   Hunter,M.R., Scourfield,E.J., Emmott,E. and Graham,S.C.
  TITLE     VPS18 recruits VPS41 to the human HOPS complex via a RING-RING
            interaction
  JOURNAL   Biochem J 474 (21), 3615-3626 (2017)
   PUBMED   28931724
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 5014)
  AUTHORS   Perini,E.D., Schaefer,R., Stoter,M., Kalaidzidis,Y. and Zerial,M.
  TITLE     Mammalian CORVET is required for fusion and conversion of distinct
            early endosome subpopulations
  JOURNAL   Traffic 15 (12), 1366-1389 (2014)
   PUBMED   25266290
REFERENCE   7  (bases 1 to 5014)
  AUTHORS   Cai,D.C., Fonteijn,H., Guadalupe,T., Zwiers,M., Wittfeld,K.,
            Teumer,A., Hoogman,M., Arias-Vasquez,A., Yang,Y., Buitelaar,J.,
            Fernandez,G., Brunner,H.G., van Bokhoven,H., Franke,B.,
            Hegenscheid,K., Homuth,G., Fisher,S.E., Grabe,H.J., Francks,C. and
            Hagoort,P.
  TITLE     A genome-wide search for quantitative trait loci affecting the
            cortical surface area and thickness of Heschl's gyrus
  JOURNAL   Genes Brain Behav 13 (7), 675-685 (2014)
   PUBMED   25130324
REFERENCE   8  (bases 1 to 5014)
  AUTHORS   Antoni,G., Oudot-Mellakh,T., Dimitromanolakis,A., Germain,M.,
            Cohen,W., Wells,P., Lathrop,M., Gagnon,F., Morange,P.E. and
            Tregouet,D.A.
  TITLE     Combined analysis of three genome-wide association studies on vWF
            and FVIII plasma levels
  JOURNAL   BMC Med Genet 12, 102 (2011)
   PUBMED   21810271
  REMARK    Publication Status: Online-Only
REFERENCE   9  (bases 1 to 5014)
  AUTHORS   Leparc,G.G. and Mitra,R.D.
  TITLE     Non-EST-based prediction of novel alternatively spliced cassette
            exons with cell signaling function in Caenorhabditis elegans and
            human
  JOURNAL   Nucleic Acids Res 35 (10), 3192-3202 (2007)
   PUBMED   17452356
REFERENCE   10 (bases 1 to 5014)
  AUTHORS   Colland,F., Jacq,X., Trouplin,V., Mougin,C., Groizeleau,C.,
            Hamburger,A., Meil,A., Wojcik,J., Legrain,P. and Gauthier,J.M.
  TITLE     Functional proteomics mapping of a human signaling pathway
  JOURNAL   Genome Res 14 (7), 1324-1332 (2004)
   PUBMED   15231748
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            DA218768.1, DA340096.1, AK056661.1, AB018347.1, AC117436.6 and
            BI914013.1.
            
            On Feb 27, 2020 this sequence version replaced NM_001009921.2.
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (a). Variants 1, 3, 4, 5, and 6 all encode the same isoform (a).
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC140768.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968968, SAMEA2144335
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000625842.3/ ENSP00000487164.1
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-36                DA218768.1         30-65
            37-623              DA340096.1         17-603
            624-1239            AK056661.1         619-1234
            1240-2514           AB018347.1         442-1716
            2515-2907           AK056661.1         2510-2902
            2908-2908           AC117436.6         91976-91976         c
            2909-2933           AK056661.1         2904-2928
            2934-3570           AB018347.1         2136-2772
            3571-3678           BI914013.1         520-627
            3679-3747           BI914013.1         629-697
            3748-5014           AB018347.1         2950-4216
FEATURES             Location/Qualifiers
     source          1..5014
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3q27.2"
     gene            1..5014
                     /gene="VPS8"
                     /gene_synonym="KIAA0804"
                     /note="VPS8 subunit of CORVET complex"
                     /db_xref="GeneID:23355"
                     /db_xref="HGNC:HGNC:29122"
                     /db_xref="MIM:618366"
     exon            1..60
                     /gene="VPS8"
                     /gene_synonym="KIAA0804"
                     /inference="alignment:Splign:2.1.0"
     exon            61..301
                     /gene="VPS8"
                     /gene_synonym="KIAA0804"
                     /inference="alignment:Splign:2.1.0"
     CDS             149..4435
                     /gene="VPS8"
                     /gene_synonym="KIAA0804"
                     /note="isoform a is encoded by transcript variant 1;
                     vacuolar protein sorting-associated protein 8 homolog;
                     vacuolar protein sorting 8 homolog; VPS8, CORVET complex
                     subunit"
                     /codon_start=1
                     /product="vacuolar protein sorting-associated protein 8
                     homolog isoform a"
                     /protein_id="NP_001009921.1"
                     /db_xref="CCDS:CCDS46971.1"
                     /db_xref="GeneID:23355"
                     /db_xref="HGNC:HGNC:29122"
                     /db_xref="MIM:618366"
                     /translation="MENEPDHENVEQSLCAKTSEEELNKSFNLEASLSKFSYIDMDKE
                     LEFKNDLIDDKEFDIPQVDTPPTLESILNETDDEDESFILEDPTLLNIDTIDSHSYDT
                     SSVASSDSGDRTNLKRKKKLPDSFSLHGSVMRHSLLKGISAQIVSAADKVDAGLPTAI
                     AVSSLIAVGTSHGLALIFGKDQNQALRLCLGSTSVGGQYGAISALSINNDCSRLLCGF
                     AKGQITMWDLASGKLLRSITDAHPPGTAILHIKFTDDPTLAICNDSGGSVFELTFKRV
                     MGVRTCESRCLFSGSKGEVCCIEPLHSKPELKDHPITQFSLLAMASLTKILVIGLKPS
                     LKVWMTFPYGRMDPSSVPLLAWHFVAVQNYVNPMLAFCRGDVVHFLLVKRDESGAIHV
                     TKQKHLHLYYDLINFTWINSRTVVLLDSVEKLHVIDRQTQEELETVEISEVQLVYNSS
                     HFKSLATGGNVSQALALVGEKACYQSISSYGGQIFYLGTKSVYVMMLRSWRERVDHLL
                     KQDCLTEALALAWSFHEGKAKAVVGLSGDASKRKAIVADRMVEILFHYADRALKKCPD
                     QGKIQVMEQHFQDMVPVIVDYCLLLQRKDLLFSQMYDKLSENSVAKGVFLECLEPYIL
                     SDKLVGITPQVMKDLIVHFQDKKLMENVEALIVHMDITSLDIQQVVLMCWENRLYDAM
                     IYVYNRGMNEFISPMEKLFRVIAPPLNAGKTLTDEQVVMGNKLLVYISCCLAGRAYPL
                     GDIPEDLVPLVKNQVFEFLIRLHSAEASPEEEIYPYIRTLLHFDTREFLNVLALTFED
                     FKNDKQAVEYQQRIVDILLKVMVENSDFTPSQVGCLFTFLARQLAKPDNTLFVNRTLF
                     DQVLEFLCSPDDDSRHSERQQVLLELLQAGGIVQFEESRLIRMAEKAEFYQICEFMYE
                     REHQYDKIIDCYLRDPLREEEVFNYIHNILSIPGHSAEEKQSVWQKAMDHIEELVSLK
                     PCKAAELVATHFSGHIETVIKKLQNQVLLFKFLRSLLDPREGIHVNQELLQISPCITE
                     QFIELLCQFNPTQVIETLQVLECYRLEETIQITQKYQLHEVTAYLLEKKGDIHGAFLI
                     MLERLQSKLQEVTHQGENTKEDPSLKDVEDTMVETIALCQRNSHNLNQQQREALWFPL
                     LEAMMAPQKLSSSAIPHLHSEALKSLTMQVLNSMAAFIALPSILQRILQDPVYGKGKL
                     GEIQGLILGMLDTFNYEQTLLETTTSLLNQDLHWSLCNLRASVTRGLNPKQDYCSICL
                     QQYKRRQEMADEIIVFSCGHLYHSFCLQNKECTVEFEGQTRWTCYKCSSSNKVGKLSE
                     NSSEIKKGRITPSQVKMSPSYHQSKGDPTAKKGTSEPVLDPQQIQAFDQLCRLYRGSS
                     RLALLTELSQNRSSESYRPFSGSQSAPAFNSIFQNENFQLQLIPPPVTED"
     exon            302..370
                     /gene="VPS8"
                     /gene_synonym="KIAA0804"
                     /inference="alignment:Splign:2.1.0"
     exon            371..501
                     /gene="VPS8"
                     /gene_synonym="KIAA0804"
                     /inference="alignment:Splign:2.1.0"
     exon            502..595
                     /gene="VPS8"
                     /gene_synonym="KIAA0804"
                     /inference="alignment:Splign:2.1.0"
     exon            596..628
                     /gene="VPS8"
                     /gene_synonym="KIAA0804"
                     /inference="alignment:Splign:2.1.0"
     exon            629..683
                     /gene="VPS8"
                     /gene_synonym="KIAA0804"
                     /inference="alignment:Splign:2.1.0"
     exon            684..689
                     /gene="VPS8"
                     /gene_synonym="KIAA0804"
                     /inference="alignment:Splign:2.1.0"
     exon            690..814
                     /gene="VPS8"
                     /gene_synonym="KIAA0804"
                     /inference="alignment:Splign:2.1.0"
     exon            815..901
                     /gene="VPS8"
                     /gene_synonym="KIAA0804"
                     /inference="alignment:Splign:2.1.0"
     exon            902..969
                     /gene="VPS8"
                     /gene_synonym="KIAA0804"
                     /inference="alignment:Splign:2.1.0"
     exon            970..1123
                     /gene="VPS8"
                     /gene_synonym="KIAA0804"
                     /inference="alignment:Splign:2.1.0"
     exon            1124..1183
                     /gene="VPS8"
                     /gene_synonym="KIAA0804"
                     /inference="alignment:Splign:2.1.0"
     exon            1184..1291
                     /gene="VPS8"
                     /gene_synonym="KIAA0804"
                     /inference="alignment:Splign:2.1.0"
     exon            1292..1372
                     /gene="VPS8"
                     /gene_synonym="KIAA0804"
                     /inference="alignment:Splign:2.1.0"
     exon            1373..1543
                     /gene="VPS8"
                     /gene_synonym="KIAA0804"
                     /inference="alignment:Splign:2.1.0"
     exon            1544..1618
                     /gene="VPS8"
                     /gene_synonym="KIAA0804"
                     /inference="alignment:Splign:2.1.0"
     exon            1619..1654
                     /gene="VPS8"
                     /gene_synonym="KIAA0804"
                     /inference="alignment:Splign:2.1.0"
     exon            1655..1745
                     /gene="VPS8"
                     /gene_synonym="KIAA0804"
                     /inference="alignment:Splign:2.1.0"
     exon            1746..1792
                     /gene="VPS8"
                     /gene_synonym="KIAA0804"
                     /inference="alignment:Splign:2.1.0"
     exon            1793..1882
                     /gene="VPS8"
                     /gene_synonym="KIAA0804"
                     /inference="alignment:Splign:2.1.0"
     exon            1883..1929
                     /gene="VPS8"
                     /gene_synonym="KIAA0804"
                     /inference="alignment:Splign:2.1.0"
     exon            1930..2152
                     /gene="VPS8"
                     /gene_synonym="KIAA0804"
                     /inference="alignment:Splign:2.1.0"
     exon            2153..2242
                     /gene="VPS8"
                     /gene_synonym="KIAA0804"
                     /inference="alignment:Splign:2.1.0"
     exon            2243..2294
                     /gene="VPS8"
                     /gene_synonym="KIAA0804"
                     /inference="alignment:Splign:2.1.0"
     exon            2295..2337
                     /gene="VPS8"
                     /gene_synonym="KIAA0804"
                     /inference="alignment:Splign:2.1.0"
     exon            2338..2410
                     /gene="VPS8"
                     /gene_synonym="KIAA0804"
                     /inference="alignment:Splign:2.1.0"
     exon            2411..2530
                     /gene="VPS8"
                     /gene_synonym="KIAA0804"
                     /inference="alignment:Splign:2.1.0"
     exon            2531..2602
                     /gene="VPS8"
                     /gene_synonym="KIAA0804"
                     /inference="alignment:Splign:2.1.0"
     exon            2603..2722
                     /gene="VPS8"
                     /gene_synonym="KIAA0804"
                     /inference="alignment:Splign:2.1.0"
     exon            2723..2779
                     /gene="VPS8"
                     /gene_synonym="KIAA0804"
                     /inference="alignment:Splign:2.1.0"
     exon            2780..2862
                     /gene="VPS8"
                     /gene_synonym="KIAA0804"
                     /inference="alignment:Splign:2.1.0"
     exon            2863..2947
                     /gene="VPS8"
                     /gene_synonym="KIAA0804"
                     /inference="alignment:Splign:2.1.0"
     exon            2948..3046
                     /gene="VPS8"
                     /gene_synonym="KIAA0804"
                     /inference="alignment:Splign:2.1.0"
     exon            3047..3136
                     /gene="VPS8"
                     /gene_synonym="KIAA0804"
                     /inference="alignment:Splign:2.1.0"
     exon            3137..3183
                     /gene="VPS8"
                     /gene_synonym="KIAA0804"
                     /inference="alignment:Splign:2.1.0"
     exon            3184..3331
                     /gene="VPS8"
                     /gene_synonym="KIAA0804"
                     /inference="alignment:Splign:2.1.0"
     exon            3332..3421
                     /gene="VPS8"
                     /gene_synonym="KIAA0804"
                     /inference="alignment:Splign:2.1.0"
     exon            3422..3464
                     /gene="VPS8"
                     /gene_synonym="KIAA0804"
                     /inference="alignment:Splign:2.1.0"
     exon            3465..3568
                     /gene="VPS8"
                     /gene_synonym="KIAA0804"
                     /inference="alignment:Splign:2.1.0"
     exon            3569..3650
                     /gene="VPS8"
                     /gene_synonym="KIAA0804"
                     /inference="alignment:Splign:2.1.0"
     exon            3651..3733
                     /gene="VPS8"
                     /gene_synonym="KIAA0804"
                     /inference="alignment:Splign:2.1.0"
     exon            3734..3814
                     /gene="VPS8"
                     /gene_synonym="KIAA0804"
                     /inference="alignment:Splign:2.1.0"
     exon            3815..3984
                     /gene="VPS8"
                     /gene_synonym="KIAA0804"
                     /inference="alignment:Splign:2.1.0"
     exon            3985..4150
                     /gene="VPS8"
                     /gene_synonym="KIAA0804"
                     /inference="alignment:Splign:2.1.0"
     exon            4151..4204
                     /gene="VPS8"
                     /gene_synonym="KIAA0804"
                     /inference="alignment:Splign:2.1.0"
     exon            4205..4285
                     /gene="VPS8"
                     /gene_synonym="KIAA0804"
                     /inference="alignment:Splign:2.1.0"
     exon            4286..5014
                     /gene="VPS8"
                     /gene_synonym="KIAA0804"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4991..4996
                     /regulatory_class="polyA_signal_sequence"
                     /gene="VPS8"
                     /gene_synonym="KIAA0804"
                     /note="hexamer: AATAAA"
     polyA_site      5014
                     /gene="VPS8"
                     /gene_synonym="KIAA0804"
                     /note="major polyA site"
ORIGIN      
        1 agagcagtgg gtgcggtcac gtgggccggc ggtccacggc cggaatggca gcaagctcag
       61 agataatcat tcaggtcttc gtgagctaag gccaaacaaa caaaaaacac ttgctttgat
      121 ggactgaata ctgttattag aagtaaatat ggaaaatgaa ccagaccatg aaaatgtgga
      181 acagagcctc tgtgccaaga cgagcgaaga agagctgaat aagtctttca atctagaagc
      241 ttcactttca aaattctctt acatagatat ggacaaggaa ctggagttca aaaatgatct
      301 gattgatgac aaggagtttg atattcctca agttgatact cctccaacac tggaaagcat
      361 actaaatgag actgatgatg aagatgagtc ttttattctt gaggatccta cattgttaaa
      421 cattgatact attgattctc actcctatga tacttcatct gtggcaagct cagatagtgg
      481 tgacaggacc aacttaaaaa ggaagaagaa attacctgat tctttttcac ttcatggatc
      541 agttatgcgc cattcacttt tgaagggaat ttctgcccag atagtgtctg cagctgacaa
      601 agtagatgct ggcttgccta cagcaattgc agtatccagt ctgatagcag tgggtacatc
      661 tcatggattg gctttaatat ttggaaaaga tcagaatcaa gctttgcgac tctgtctggg
      721 tagcactagt gttggaggtc agtatggcgc tatctctgcc ctcagtatca acaatgattg
      781 ctcaagactt ctttgtggct ttgctaaagg acagatcacc atgtgggatt tggccagtgg
      841 aaaacttcta agatcaataa cagatgctca tcctccagga acagcaatat tgcatatcaa
      901 gtttacagat gatccaactc ttgcaatttg caacgacagc ggaggctctg tttttgaatt
      961 gacatttaag agagtgatgg gagtgagaac ctgtgaatct agatgtctgt tcagtggttc
     1021 caagggtgaa gtctgctgta ttgagcctct gcattctaag cctgagttga aagatcatcc
     1081 catcacacag ttttcattat tggccatggc atccttgaca aaaatactgg tcattggatt
     1141 gaaaccatcc ttgaaagtat ggatgacttt tccctatggc cggatggatc cttccagtgt
     1201 gccactgctg gcctggcact ttgtagcagt acaaaattac gtgaatccca tgcttgcctt
     1261 ctgcagagga gatgttgttc attttctatt ggtaaagaga gatgaatctg gagcaataca
     1321 tgttactaag caaaagcatc ttcacctata ctatgacctc atcaacttta cctggataaa
     1381 ttcacgcaca gttgtgctct tagacagcgt agagaagttg catgtgattg atcggcaaac
     1441 acaagaggaa ttggagacag tggagatctc agaagttcag ctggtctaca atagcagcca
     1501 tttcaaatca ctagccactg gaggaaatgt tagccaggca ctggctttgg ttggagagaa
     1561 ggcttgttat caatccatca gtagctatgg tggtcagatc ttttatttgg ggacaaaatc
     1621 tgtttatgtg atgatgctga ggagctggag agagagagtg gatcatctcc tgaaacaaga
     1681 ttgtcttaca gaagcgttgg ctcttgcgtg gtctttccat gaaggaaaag caaaagcagt
     1741 agtgggatta tcaggggatg ccagtaagcg aaaggctatt gttgcagacc ggatggtaga
     1801 aatcctattc cattatgcag atcgagctct gaaaaagtgc ccagaccaag gaaaaatcca
     1861 agtgatggag cagcattttc aggatatggt gcctgtcata gttgattact gccttctgct
     1921 gcagcgaaag gatcttttat ttagtcagat gtatgataaa ttaagtgaga attcagtggc
     1981 caaaggagta tttttggagt gccttgagcc atatatttta agtgataaat tggtgggaat
     2041 cacaccccaa gtaatgaaag acttgattgt tcatttccaa gataaaaaat taatggaaaa
     2101 tgtggaagcg ctcattgtac atatggatat caccagccta gatattcagc aggtagttct
     2161 catgtgttgg gaaaatcgtt tatatgatgc tatgatctat gtctacaaca gaggcatgaa
     2221 tgaatttatt agtccaatgg agaaactttt cagagtcatt gctcctcctc tgaatgcagg
     2281 aaaaacacta acagatgaac aagttgttat gggcaataag ctccttgtat atattagctg
     2341 ttgtctagca ggtcgtgcct atccccttgg tgacatccct gaagatctgg ttcccttggt
     2401 taaaaaccag gtttttgaat ttctaattcg cctgcattca gcagaggctt ctcctgagga
     2461 agaaatctat ccttacattc ggactttgct acattttgac acaagagaat ttctaaatgt
     2521 attggcactg acttttgaag attttaaaaa tgacaagcaa gctgtggaat atcaacagcg
     2581 aattgtggat attttgttga aagttatggt ggagaattca gactttaccc cctcacaagt
     2641 aggatgtctc tttaccttcc ttgctcggca gcttgcaaag cctgacaaca ccttgtttgt
     2701 aaacagaaca ctttttgatc aggtccttga attcctttgt agtcctgacg atgactcccg
     2761 acactctgaa agacagcagg tccttttaga attgctgcag gctggaggca tagttcaatt
     2821 tgaagagagt cgactcatcc ggatggcaga aaaagctgag ttctatcaaa tttgtgaatt
     2881 tatgtatgaa agagaacacc aatatgataa aattattgat tgctacttac gtgaccctct
     2941 gcgagaggaa gaagtcttta attacattca caatatctta tccattcccg gacacagtgc
     3001 agaggagaag cagtctgtat ggcagaaagc aatggatcat attgaggaac tcgtgtccct
     3061 gaagccttgt aaagctgcgg agctggttgc cacccacttt tctggacata ttgaaacggt
     3121 cattaaaaaa cttcagaacc aggttttgct tttcaaattt ttgaggagtc ttcttgaccc
     3181 aagggaaggt attcatgtaa atcaagaatt actgcaaata tctccttgta tcacagagca
     3241 gttcattgag ctgttgtgtc agttcaaccc aacccaagtt atagagactc tgcaagtcct
     3301 tgagtgctac cgtctggaag aaactattca gattactcag aagtatcaac ttcatgaagt
     3361 caccgcttat ctattggaaa agaaaggaga tattcatggt gccttcctaa taatgttaga
     3421 gagactacaa agcaaacttc aagaggtaac acatcaaggt gaaaatacca aagaggatcc
     3481 ctcattgaag gatgttgaag atactatggt ggagaccatt gctctttgcc agagaaattc
     3541 acataatttg aaccagcagc aacgtgaggc actttggttt ccgttattgg aggcaatgat
     3601 ggcccctcag aagctgtcca gttcagccat tcctcatcta cactctgaag ctctgaagtc
     3661 tttgaccatg caagttttaa atagcatggc agcatttatt gcccttccat caatcttgca
     3721 aagaatctta caggatccag tttatggaaa aggaaaactt ggagaaatcc agggacttat
     3781 cttgggaatg ttagatacct ttaactatga acaaaccctg ctggaaacaa caaccagcct
     3841 tctaaaccaa gatctccatt ggtcattgtg taacctgaga gcttcggtca ccagaggact
     3901 gaatcccaaa caagattact gctctatatg tttgcagcag tacaagagac gccaagaaat
     3961 ggctgatgaa ataattgtct ttagctgtgg ccatttgtat cactcattct gcctacaaaa
     4021 caaagaatgc actgtggaat ttgagggcca aacaagatgg acatgctaca aatgcagttc
     4081 aagtaacaaa gtaggaaaac tcagtgaaaa ttcatctgaa attaaaaagg gaaggataac
     4141 cccatcacag gtaaaaatgt ctccatcgta tcatcagtcc aaaggggatc ccactgctaa
     4201 aaagggaacc tcagaacctg ttctggatcc acagcaaatc caagcatttg atcagctttg
     4261 ccgtctctac cgaggaagct ccaggctggc tctcctcacg gaactctccc agaatcgcag
     4321 cagcgagagc tataggccat tcagtggctc gcagagtgct cctgctttca acagcatctt
     4381 ccagaatgag aacttccagc tgcagctcat tcctccacct gtgactgagg attgatgact
     4441 ccatggagcc tggcccagga gaaccagaga tgatcccgag gcagctgggg agaggccccg
     4501 cctctggtgg gcttggcctc caccacctcc cacgcttctg agaagaggtt ccaaattggg
     4561 cttctgtgcc cagagcgtcc acagcaccat tcccagtgta gactcccagt cttctccaca
     4621 ttgctgtcat ggcgtcagtt caccagactc attgattttg ttttgcttgt taagcaaagg
     4681 aatgtcacat acctctgtcc agctttttag gaaatacatt tcgcctattg cgactttttc
     4741 catttaccct gaagcctaga aagtaggtgg aactcacaca aatggcattc cagagtctgc
     4801 catactccgt ctcctccagc tgctggataa tacagaggaa cttcaacttc tacagggaac
     4861 agtggttggc caggctgcag tataactgaa gcatgccttg gagagagcag acactgtggg
     4921 ggccagggcc atctcccttt aatgtgttca tgttaaaacc tatttgagtg taagacttgc
     4981 cctttctaac aataaatgct ccgtgtttaa gttc
//
