LOCUS       NM_016516               4725 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens VPS54 subunit of GARP complex (VPS54), transcript
            variant 1, mRNA.
ACCESSION   NM_016516
VERSION     NM_016516.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4725)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   2  (bases 1 to 4725)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   3  (bases 1 to 4725)
  AUTHORS   Schindler,C., Chen,Y., Pu,J., Guo,X. and Bonifacino,J.S.
  TITLE     EARP is a multisubunit tethering complex involved in endocytic
            recycling
  JOURNAL   Nat Cell Biol 17 (5), 639-650 (2015)
   PUBMED   25799061
REFERENCE   4  (bases 1 to 4725)
  AUTHORS   Rosa-Ferreira,C., Christis,C., Torres,I.L. and Munro,S.
  TITLE     The small G protein Arl5 contributes to endosome-to-Golgi traffic
            by aiding the recruitment of the GARP complex to the Golgi
  JOURNAL   Biol Open 4 (4), 474-481 (2015)
   PUBMED   25795912
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 4725)
  AUTHORS   Goldstein,J.I., Jarskog,L.F., Hilliard,C., Alfirevic,A., Duncan,L.,
            Fourches,D., Huang,H., Lek,M., Neale,B.M., Ripke,S., Shianna,K.,
            Szatkiewicz,J.P., Tropsha,A., van den Oord,E.J., Cascorbi,I.,
            Dettling,M., Gazit,E., Goff,D.C., Holden,A.L., Kelly,D.L.,
            Malhotra,A.K., Nielsen,J., Pirmohamed,M., Rujescu,D., Werge,T.,
            Levy,D.L., Josiassen,R.C., Kennedy,J.L., Lieberman,J.A., Daly,M.J.
            and Sullivan,P.F.
  TITLE     Clozapine-induced agranulocytosis is associated with rare HLA-DQB1
            and HLA-B alleles
  JOURNAL   Nat Commun 5, 4757 (2014)
   PUBMED   25187353
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 4725)
  AUTHORS   Perez-Victoria,F.J., Mardones,G.A. and Bonifacino,J.S.
  TITLE     Requirement of the human GARP complex for mannose
            6-phosphate-receptor-dependent sorting of cathepsin D to lysosomes
  JOURNAL   Mol Biol Cell 19 (6), 2350-2362 (2008)
   PUBMED   18367545
  REMARK    GeneRIF: Missorting of cathepsin D in GARP-depleted cells results
            from accumulation of recycling MPRs in a population of light, small
            vesicles downstream of endosomes.
REFERENCE   7  (bases 1 to 4725)
  AUTHORS   Liewen,H., Meinhold-Heerlein,I., Oliveira,V., Schwarzenbacher,R.,
            Luo,G., Wadle,A., Jung,M., Pfreundschuh,M. and Stenner-Liewen,F.
  TITLE     Characterization of the human GARP (Golgi associated retrograde
            protein) complex
  JOURNAL   Exp Cell Res 306 (1), 24-34 (2005)
   PUBMED   15878329
  REMARK    GeneRIF: Golgi associated retrograde protein complex, consisting of
            the Vps52, Vps53, and Vps54 proteins, shows significant
            conservation between various species but diversification and
            specialization result in important differences in human cells.
REFERENCE   8  (bases 1 to 4725)
  AUTHORS   Fuchs,S., Resch,K., Thiel,C., Ulbrich,M., Platzer,M., Jockusch,H.
            and Schmitt-John,T.
  TITLE     Comparative transcription map of the wobbler critical region on
            mouse chromosome 11 and the homologous region on human chromosome
            2p13-14
  JOURNAL   BMC Genet 3, 14 (2002)
   PUBMED   12174196
REFERENCE   9  (bases 1 to 4725)
  AUTHORS   Walter,L., Stark,S., Helou,K., Flugge,P., Levan,G. and Gunther,E.
  TITLE     Identification, characterization and cytogenetic mapping of a yeast
            Vps54 homolog in rat and mouse
  JOURNAL   Gene 285 (1-2), 213-220 (2002)
   PUBMED   12039048
  REMARK    GeneRIF: PMID:12039048 mostly pertains to mouse and rat but
            contains some general comments about the structure and function of
            Vps54 homolog in other species, including human.
REFERENCE   10 (bases 1 to 4725)
  AUTHORS   Canestri,F.
  TITLE     A proposed clinical application of a model of CO2 laser radiation
            induced damage craters
  JOURNAL   J Med Eng Technol 12 (3), 112-117 (1988)
   PUBMED   3172165
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC092588.2, BC030275.1,
            AL514510.3, AF102177.1, BC041868.1, AK002205.1 and AW452246.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_016516.2.
            
            Summary: This gene encodes for a protein that in yeast forms part
            of a trimeric vacuolar-protein-sorting complex that is required for
            retrograde transport of proteins from prevacuoles to the late Golgi
            compartment. As in yeast, mammalian Vps54 proteins contain a
            coiled-coil region and dileucine motifs. Alternative splicing
            results in multiple transcript variants encoding different isoforms
            [provided by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (1) encodes the longer isoform
            (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803615.194861.1,
                                           ERR3218371.429475.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000272322.9/ ENSP00000272322.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-348               AC092588.2         131612-131959       c
            349-351             BC030275.1         5-7
            352-647             AL514510.3         110-405
            648-699             AF102177.1         157-208
            700-3814            BC030275.1         319-3433
            3815-3974           BC041868.1         3435-3594
            3975-4267           AK002205.1         1732-2024
            4268-4342           AW452246.1         398-472
            4343-4725           AC092588.2         4681-5063           c
FEATURES             Location/Qualifiers
     source          1..4725
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2p15-p14"
     gene            1..4725
                     /gene="VPS54"
                     /gene_synonym="HCC8; hVps54L; PPP1R164; SLP-8p; VPS54L;
                     WR"
                     /note="VPS54 subunit of GARP complex"
                     /db_xref="GeneID:51542"
                     /db_xref="HGNC:HGNC:18652"
                     /db_xref="MIM:614633"
     exon            1..491
                     /gene="VPS54"
                     /gene_synonym="HCC8; hVps54L; PPP1R164; SLP-8p; VPS54L;
                     WR"
                     /inference="alignment:Splign:2.1.0"
     exon            492..647
                     /gene="VPS54"
                     /gene_synonym="HCC8; hVps54L; PPP1R164; SLP-8p; VPS54L;
                     WR"
                     /inference="alignment:Splign:2.1.0"
     CDS             512..3445
                     /gene="VPS54"
                     /gene_synonym="HCC8; hVps54L; PPP1R164; SLP-8p; VPS54L;
                     WR"
                     /note="isoform 1 is encoded by transcript variant 1; tumor
                     antigen SLP-8p; vacuolar protein sorting-associated
                     protein 54; tumor antigen HOM-HCC-8; hepatocellular
                     carcinoma protein 8; protein phosphatase 1, regulatory
                     subunit 164; vacuolar protein sorting 54 homolog; VPS54,
                     GARP complex subunit"
                     /codon_start=1
                     /product="vacuolar protein sorting-associated protein 54
                     isoform 1"
                     /protein_id="NP_057600.2"
                     /db_xref="CCDS:CCDS33208.1"
                     /db_xref="GeneID:51542"
                     /db_xref="HGNC:HGNC:18652"
                     /db_xref="MIM:614633"
                     /translation="MASSHSSSPVPQGSSSDVFFKIEVDPSKHIRPVPSLPDVCPKEP
                     TGDSHSLYVAPSLVTDQHRWTVYHSKVNLPAALNDPRLAKRESDFFTKTWGLDFVDTE
                     VIPSFYLPQISKEHFTVYQQEISQREKIHERCKNICPPKDTFERTLLHTHDKSRTDLE
                     QVPKIFMKPDFALDDSLTFNSVLPWSHFNTAGGKGNRDAASSKLLQEKLSHYLDIVEV
                     NIAHQISLRSEAFFHAMTSQHELQDYLRKTSQAVKMLRDKIAQIDKVMCEGSLHILRL
                     ALTRNNCVKVYNKLKLMATVHQTQPTVQVLLSTSEFVGALDLIATTQEVLQQELQGIH
                     SFRHLGSQLCELEKLIDKMMIAEFSTYSHSDLNRPLEDDCQVLEEERLISLVFGLLKQ
                     RKLNFLEIYGEKMVITAKNIIKQCVINKVSQTEEIDTDVVVKLADQMRMLNFPQWFDL
                     LKDIFSKFTIFLQRVKATLNIIHSVVLSVLDKNQRTRELEEISQQKNAAKDNSLDTEV
                     AYLIHEGMFISDAFGEGELTPIAVDTTSQRNASPNSEPCSSDSVSEPECTTDSSSSKE
                     HTSSSAIPGGVDIMVSEDMKLTDSELGKLANNIQELLYSASDICHDRAVKFLMSRAKD
                     GFLEKLNSMEFITLSRLMETFILDTEQICGRKSTSLLGALQSQAIKFVNRFHEERKTK
                     LSLLLDNERWKQADVPAEFQDLVDSLSDGKIALPEKKSGATEERKPAEVLIVEGQQYA
                     VVGTVLLLIRIILEYCQCVDNIPSVTTDMLTRLSDLLKYFNSRSCQLVLGAGALQVVG
                     LKTITTKNLALSSRCLQLIVHYIPVIRAHFEARLPPKQYSMLRHFDHITKDYHDHIAE
                     ISAKLVAIMDSLFDKLLSKYEVKAPVPSACFRNICKQMTKMHEAIFDLLPEEQTQMLF
                     LRINASYKLHLKKQLSHLNVINDGGPQNGLVTADVAFYTGNLQALKGLKDLDLNMAEI
                     WEQKR"
     exon            648..889
                     /gene="VPS54"
                     /gene_synonym="HCC8; hVps54L; PPP1R164; SLP-8p; VPS54L;
                     WR"
                     /inference="alignment:Splign:2.1.0"
     exon            890..968
                     /gene="VPS54"
                     /gene_synonym="HCC8; hVps54L; PPP1R164; SLP-8p; VPS54L;
                     WR"
                     /inference="alignment:Splign:2.1.0"
     exon            969..1003
                     /gene="VPS54"
                     /gene_synonym="HCC8; hVps54L; PPP1R164; SLP-8p; VPS54L;
                     WR"
                     /inference="alignment:Splign:2.1.0"
     exon            1004..1135
                     /gene="VPS54"
                     /gene_synonym="HCC8; hVps54L; PPP1R164; SLP-8p; VPS54L;
                     WR"
                     /inference="alignment:Splign:2.1.0"
     exon            1136..1521
                     /gene="VPS54"
                     /gene_synonym="HCC8; hVps54L; PPP1R164; SLP-8p; VPS54L;
                     WR"
                     /inference="alignment:Splign:2.1.0"
     exon            1522..1648
                     /gene="VPS54"
                     /gene_synonym="HCC8; hVps54L; PPP1R164; SLP-8p; VPS54L;
                     WR"
                     /inference="alignment:Splign:2.1.0"
     exon            1649..1756
                     /gene="VPS54"
                     /gene_synonym="HCC8; hVps54L; PPP1R164; SLP-8p; VPS54L;
                     WR"
                     /inference="alignment:Splign:2.1.0"
     exon            1757..1812
                     /gene="VPS54"
                     /gene_synonym="HCC8; hVps54L; PPP1R164; SLP-8p; VPS54L;
                     WR"
                     /inference="alignment:Splign:2.1.0"
     exon            1813..1909
                     /gene="VPS54"
                     /gene_synonym="HCC8; hVps54L; PPP1R164; SLP-8p; VPS54L;
                     WR"
                     /inference="alignment:Splign:2.1.0"
     exon            1910..2250
                     /gene="VPS54"
                     /gene_synonym="HCC8; hVps54L; PPP1R164; SLP-8p; VPS54L;
                     WR"
                     /inference="alignment:Splign:2.1.0"
     exon            2251..2380
                     /gene="VPS54"
                     /gene_synonym="HCC8; hVps54L; PPP1R164; SLP-8p; VPS54L;
                     WR"
                     /inference="alignment:Splign:2.1.0"
     exon            2381..2562
                     /gene="VPS54"
                     /gene_synonym="HCC8; hVps54L; PPP1R164; SLP-8p; VPS54L;
                     WR"
                     /inference="alignment:Splign:2.1.0"
     exon            2563..2675
                     /gene="VPS54"
                     /gene_synonym="HCC8; hVps54L; PPP1R164; SLP-8p; VPS54L;
                     WR"
                     /inference="alignment:Splign:2.1.0"
     exon            2676..2739
                     /gene="VPS54"
                     /gene_synonym="HCC8; hVps54L; PPP1R164; SLP-8p; VPS54L;
                     WR"
                     /inference="alignment:Splign:2.1.0"
     exon            2740..2845
                     /gene="VPS54"
                     /gene_synonym="HCC8; hVps54L; PPP1R164; SLP-8p; VPS54L;
                     WR"
                     /inference="alignment:Splign:2.1.0"
     exon            2846..2933
                     /gene="VPS54"
                     /gene_synonym="HCC8; hVps54L; PPP1R164; SLP-8p; VPS54L;
                     WR"
                     /inference="alignment:Splign:2.1.0"
     exon            2934..3055
                     /gene="VPS54"
                     /gene_synonym="HCC8; hVps54L; PPP1R164; SLP-8p; VPS54L;
                     WR"
                     /inference="alignment:Splign:2.1.0"
     exon            3056..3136
                     /gene="VPS54"
                     /gene_synonym="HCC8; hVps54L; PPP1R164; SLP-8p; VPS54L;
                     WR"
                     /inference="alignment:Splign:2.1.0"
     exon            3137..3244
                     /gene="VPS54"
                     /gene_synonym="HCC8; hVps54L; PPP1R164; SLP-8p; VPS54L;
                     WR"
                     /inference="alignment:Splign:2.1.0"
     exon            3245..3339
                     /gene="VPS54"
                     /gene_synonym="HCC8; hVps54L; PPP1R164; SLP-8p; VPS54L;
                     WR"
                     /inference="alignment:Splign:2.1.0"
     exon            3340..4725
                     /gene="VPS54"
                     /gene_synonym="HCC8; hVps54L; PPP1R164; SLP-8p; VPS54L;
                     WR"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3782..3787
                     /regulatory_class="polyA_signal_sequence"
                     /gene="VPS54"
                     /gene_synonym="HCC8; hVps54L; PPP1R164; SLP-8p; VPS54L;
                     WR"
                     /note="hexamer: ATTAAA"
     polyA_site      3819
                     /gene="VPS54"
                     /gene_synonym="HCC8; hVps54L; PPP1R164; SLP-8p; VPS54L;
                     WR"
                     /note="major polyA site"
     regulatory      3950..3955
                     /regulatory_class="polyA_signal_sequence"
                     /gene="VPS54"
                     /gene_synonym="HCC8; hVps54L; PPP1R164; SLP-8p; VPS54L;
                     WR"
                     /note="hexamer: AATAAA"
     polyA_site      3974
                     /gene="VPS54"
                     /gene_synonym="HCC8; hVps54L; PPP1R164; SLP-8p; VPS54L;
                     WR"
                     /note="major polyA site"
     regulatory      4705..4710
                     /regulatory_class="polyA_signal_sequence"
                     /gene="VPS54"
                     /gene_synonym="HCC8; hVps54L; PPP1R164; SLP-8p; VPS54L;
                     WR"
                     /note="hexamer: AATAAA"
     polyA_site      4725
                     /gene="VPS54"
                     /gene_synonym="HCC8; hVps54L; PPP1R164; SLP-8p; VPS54L;
                     WR"
                     /note="major polyA site"
ORIGIN      
        1 ggattgtggg ctgggggctg cggcggcctc cggcgctgtc tccccgcacc cgaccgggcc
       61 gagccggctg ggccggcggg gtgagggaaa gcagtggagt cgggagcaga agcgctagag
      121 gcagtggtcg tggcgcggcg gcggcggctc ccctggaggc cggggatgtg ggagaggcgg
      181 tggcagcagc ggcggaggcg gcgctgcgga cccgggggaa actgctggct gacaggacac
      241 ccgggagaga cgtgagggag ccgccgctgc cacctctcac ccctgagtga agctgggctc
      301 gagagggcgg ccctgtgctc cccgggccga ctggccagcg ggcgcggggc gggggcggga
      361 acccgggctc gggcccggcc gggcgccggg cggcggcggc cgtggagcag cagcctcggt
      421 gcgacgtgga gggctggagg cggcggcgat gcactaggcc tcgctcaggg cggctgcccc
      481 gggacccgca gttgagtggt gattttatgc aatggcttca agccacagtt cttcaccagt
      541 gcctcaagga agcagcagtg atgttttctt taaaatagag gtagatccgt caaaacacat
      601 tcgacctgtg ccatcactgc cagatgtgtg tcccaaggaa cccacaggtg attcacatag
      661 tttatatgtt gccccatctc tagttacaga tcaacataga tggactgtat atcattccaa
      721 agtaaatctc ccagcagcat taaacgatcc tagattagca aaaagagaat ctgacttctt
      781 cacaaaaaca tggggattgg actttgtgga cactgaagtc ataccttcat tctacctccc
      841 acagatcagc aaggaacatt ttacagtata tcaacaggaa atctctcaga gagagaagat
      901 tcatgagaga tgcaagaata tttgtcctcc taaagatacc ttcgaaagga ctcttttaca
      961 tactcatgat aaatccagga cagatctgga gcaagtacct aagattttta tgaaaccaga
     1021 ttttgccttg gatgattcct taacttttaa ttcagtttta ccatggtctc attttaatac
     1081 tgctggtgga aaaggaaatc gtgatgcagc ttcctcaaag ttgcttcaag aaaagctgag
     1141 ccattatctg gatattgtgg aagtaaacat tgctcaccag atctctctac gttcagaagc
     1201 attttttcat gcaatgacct ctcaacacga gttgcaggac tacctcagga aaacttccca
     1261 ggctgtaaaa atgcttcgag ataaaattgc acagattgat aaagtaatgt gtgaaggatc
     1321 actccacatt ttaagactgg cacttaccag aaataattgt gttaaagtat acaataagct
     1381 gaagttaatg gccactgtac accagactca gcctacagta caggtgttat tatctacttc
     1441 tgaatttgtt ggagcattgg acttaatagc aacaacacaa gaggttctac agcaggaact
     1501 tcagggcatt cacagtttcc ggcatttggg atcacagctt tgtgaattag aaaaactgat
     1561 agataaaatg atgattgcag aattttctac ttattctcac agtgacttaa atagaccact
     1621 ggaagatgac tgtcaagttt tagaagagga aagactaata tctcttgtat ttggactttt
     1681 aaaacaaaga aagcttaatt ttttagaaat ctatggtgaa aaaatggtta ttacagcaaa
     1741 gaatatcatt aaacagtgtg tgattaataa agtttcacaa acagaagaaa tagacacaga
     1801 tgttgttgtg aagcttgcag atcagatgag aatgttgaat tttccccagt ggtttgatct
     1861 gctcaaggat attttctcta agtttacaat tttcctacag agagtgaagg caacattaaa
     1921 tatcattcac agtgttgttc tctcagttct tgacaaaaac caaaggacta gagaattgga
     1981 agagatttca caacagaaga atgctgcaaa agataattca ctggacacag aggtggctta
     2041 tttaatccat gaaggcatgt ttataagtga tgcattcggt gagggtgagc taacacctat
     2101 agcagttgac actacctctc aaagaaatgc atctccaaat agtgagccct gcagcagtga
     2161 ttctgtatcc gagccagaat gtactactga ttcttcatcc agcaaagagc acacatcatc
     2221 atctgctatt ccaggaggtg tggatattat ggtcagtgaa gatatgaaat taactgactc
     2281 agagctagga aagctggcaa ataatatcca ggaattatta tatagtgcct cagatatatg
     2341 ccatgatcga gctgtcaaat ttctcatgtc aagagcaaag gatggttttc ttgagaagct
     2401 aaattccatg gaattcataa cactttctag attaatggaa acattcattt tagacaccga
     2461 acagatctgt ggaagaaaaa gcacgtcatt acttggagca cttcagagcc aagctattaa
     2521 gtttgtaaat aggtttcatg aagagagaaa aaccaagctc agcctcctct tagacaatga
     2581 gcgctggaag caagcagatg ttcctgcaga atttcaggat cttgttgatt ctctgtcaga
     2641 tgggaagatt gctttacctg aaaaaaaatc aggagccaca gaagaaagga aaccagctga
     2701 agttcttatt gtcgagggac aacagtatgc agttgttgga accgtattgc tgttaataag
     2761 aattatcctt gaatattgcc agtgtgtgga taacatccca tctgttacta ctgacatgct
     2821 tactcgtctg tcagatttat tgaagtactt caattcaaga agttgccagt tagttcttgg
     2881 agctggtgca ctgcaagttg ttggactaaa aacgataact acaaaaaatt tggctctttc
     2941 ttcacgatgt ttgcagttaa ttgtgcacta cattcctgtg atccgggctc attttgaagc
     3001 tcgactacca cctaagcaat atagcatgct taggcatttt gatcatatca ctaaggacta
     3061 ccatgatcac atagctgaaa tatcagctaa gcttgtagcg ataatggata gcttatttga
     3121 caagctgtta tctaagtatg aagtgaaggc tcctgttcct tctgcctgtt tcaggaatat
     3181 ttgtaagcaa atgacaaaaa tgcacgaagc tatatttgat ctccttccag aagaacaaac
     3241 acagatgtta tttttaagaa ttaatgcaag ttataaactc cacttgaaaa agcagttatc
     3301 tcacttaaat gtgataaatg atggaggacc tcaaaatggg ttggtcacag cagatgtagc
     3361 tttttacact ggaaatcttc aagccttaaa aggccttaaa gatttggacc taaatatggc
     3421 cgaaatttgg gagcagaaga ggtgatgtca tcctggaaaa ctgggtagtt catctgacca
     3481 tgggatgtgt ttgttatgaa gaaaatctgg atgcctgtga ttcgagaatt gaacctgaaa
     3541 cccaaagtga actggggtgg gggaagggaa aaaggaaagt atcaagtgtt gggaaactgg
     3601 attcagtggg atctacaagg aatgtcattt ttgtgcatcc tacagtgagg agtaactgat
     3661 caggtgtcta taacattttt cattctctct ggaaacagac tcaggtttct ttggaccaaa
     3721 tccaaaagaa cacatagctg taacacagct gtagttgact agaatgctct gtatacttta
     3781 tattaaaaaa tgctttgcat ttcttccagt gcaatgaaat tcatatggtg tcccacctta
     3841 tttaatgatg gtacaattta aaatcttagt caacttctgt agaaagtttt ctctatgaaa
     3901 gtaaagctgt ttgaaaaatt attatttttt tacagatctt tctataaaaa ataaacatct
     3961 tttgattgct tggatttagg aattcaattt ttgtttcagt gaccaatgtc aagttgcagg
     4021 ctttgtgtgt tgcatattta atatttctac taccaccgta tgtcaactgg gtaaagcctt
     4081 ccagagctct ctaaatacct gaaagactta agcctttttt taagtaatta atacttaaaa
     4141 aaaaagatcc tggcatctat ttatgcaact aaatactttt cagaaatggt atataaaagg
     4201 ctacagttta atatctgtat ttttataaaa gtatgatgaa gggtttgggg tgtttttatt
     4261 tgtagcagag gtaagaatat gtattcatat aatctgccta cttttgagtt ctcatattaa
     4321 catttagata acatatgaag tatggccccc ttggatcctt gtttttaagt tactttcgat
     4381 gtgtatacga acaaagacca gggagaaaca gaacttttaa aactataggc tgctgtgttg
     4441 gggccagaaa taaggtgaca agtaaagact tttataatac ctaccttcaa aattagaatc
     4501 agcagcactg tacaaataaa gtgcctgtgc ttcacctcac accaagaatc ttgcctaacc
     4561 ctgtcagagt gcctaatatc ctgtggtgat tatgtataat gaaacagtct tattttgtgc
     4621 tctttggttt cttaaaagga aagcttgttt tctttccttt atcattacag aggtatgtct
     4681 ttggttctta aactatctgt ctaaaataaa gctgtaaaaa attaa
//
