LOCUS       NM_001128159           13089 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens VPS53 subunit of GARP complex (VPS53), transcript
            variant 1, mRNA.
ACCESSION   NM_001128159
VERSION     NM_001128159.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 13089)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   2  (bases 1 to 13089)
  AUTHORS   Ilyas,M., Efthymiou,S., Salpietro,V., Noureen,N., Zafar,F.,
            Rauf,S., Mir,A. and Houlden,H.
  TITLE     Novel variants underlying autosomal recessive intellectual
            disability in Pakistani consanguineous families
  JOURNAL   BMC Med Genet 21 (1), 59 (2020)
   PUBMED   32209057
  REMARK    GeneRIF: Novel and deleterious variants in VPS53, GLB1, and MLC1,
            genes previously associated with variable neurodevelopmental
            anomalies, were found to segregate with intellectual disabilities
            in the three families.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 13089)
  AUTHORS   Hausman-Kedem,M., Ben-Shachar,S., Menascu,S., Geva,K., Sagie,L. and
            Fattal-Valevski,A.
  TITLE     VPS53 gene is associated with a new phenotype of complicated
            hereditary spastic paraparesis
  JOURNAL   Neurogenetics 20 (4), 187-195 (2019)
   PUBMED   31418091
  REMARK    GeneRIF: VPS53 gene is associated with a new phenotype of
            complicated hereditary spastic paraparesis.
REFERENCE   4  (bases 1 to 13089)
  AUTHORS   Xie,X.H., Chen,Y.R., Jin,S., Ma,Y.S., Tian,X.D. and Yang,Y.M.
  TITLE     HCCS1 inhibits the stemness of human pancreatic cancer stem-like
            cells
  JOURNAL   Eur Rev Med Pharmacol Sci 23 (5), 2125-2131 (2019)
   PUBMED   30915757
  REMARK    GeneRIF: HCCS1 inhibits the stemness of human pancreatic cancer
            stem-like cells.
REFERENCE   5  (bases 1 to 13089)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Paulo,J.A., Cannon,J.R., Ting,L.,
            Baltier,K., Colby,G., Gebreab,F., Gygi,M.P., Parzen,H., Szpyt,J.,
            Tam,S., Zarraga,G., Pontano-Vaites,L., Swarup,S., White,A.E.,
            Schweppe,D.K., Rad,R., Erickson,B.K., Obar,R.A., Guruharsha,K.G.,
            Li,K., Artavanis-Tsakonas,S., Gygi,S.P. and Harper,J.W.
  TITLE     Architecture of the human interactome defines protein communities
            and disease networks
  JOURNAL   Nature 545 (7655), 505-509 (2017)
   PUBMED   28514442
REFERENCE   6  (bases 1 to 13089)
  AUTHORS   Liewen,H., Meinhold-Heerlein,I., Oliveira,V., Schwarzenbacher,R.,
            Luo,G., Wadle,A., Jung,M., Pfreundschuh,M. and Stenner-Liewen,F.
  TITLE     Characterization of the human GARP (Golgi associated retrograde
            protein) complex
  JOURNAL   Exp Cell Res 306 (1), 24-34 (2005)
   PUBMED   15878329
  REMARK    GeneRIF: Golgi associated retrograde protein complex, consisting of
            the Vps52, Vps53, and Vps54 proteins, shows significant
            conservation between various species but diversification and
            specialization result in important differences in human cells.
REFERENCE   7  (bases 1 to 13089)
  AUTHORS   Wan,D., Gong,Y., Qin,W., Zhang,P., Li,J., Wei,L., Zhou,X., Li,H.,
            Qiu,X., Zhong,F., He,L., Yu,J., Yao,G., Jiang,H., Qian,L., Yu,Y.,
            Shu,H., Chen,X., Xu,H., Guo,M., Pan,Z., Chen,Y., Ge,C., Yang,S. and
            Gu,J.
  TITLE     Large-scale cDNA transfection screening for genes related to cancer
            development and progression
  JOURNAL   Proc Natl Acad Sci U S A 101 (44), 15724-15729 (2004)
   PUBMED   15498874
  REMARK    Erratum:[Proc Natl Acad Sci U S A. 2004 Dec 14:101(50):17565]
REFERENCE   8  (bases 1 to 13089)
  AUTHORS   Cardoso,C., Leventer,R.J., Ward,H.L., Toyo-Oka,K., Chung,J.,
            Gross,A., Martin,C.L., Allanson,J., Pilz,D.T., Olney,A.H.,
            Mutchinick,O.M., Hirotsune,S., Wynshaw-Boris,A., Dobyns,W.B. and
            Ledbetter,D.H.
  TITLE     Refinement of a 400-kb critical region allows genotypic
            differentiation between isolated lissencephaly, Miller-Dieker
            syndrome, and other phenotypes secondary to deletions of 17p13.3
  JOURNAL   Am J Hum Genet 72 (4), 918-930 (2003)
   PUBMED   12621583
REFERENCE   9  (bases 1 to 13089)
  AUTHORS   Zhao,X., He,M., Wan,D., Ye,Y., He,Y., Han,L., Guo,M., Huang,Y.,
            Qin,W., Wang,M.W., Chong,W., Chen,J., Zhang,L., Yang,N., Xu,B.,
            Wu,M., Zuo,L. and Gu,J.
  TITLE     The minimum LOH region defined on chromosome 17p13.3 in human
            hepatocellular carcinoma with gene content analysis
  JOURNAL   Cancer Lett 190 (2), 221-232 (2003)
   PUBMED   12565177
REFERENCE   10 (bases 1 to 13089)
  AUTHORS   Canestri,F.
  TITLE     A proposed clinical application of a model of CO2 laser radiation
            induced damage craters
  JOURNAL   J Med Eng Technol 12 (3), 112-117 (1988)
   PUBMED   3172165
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK092532.1, AL701573.1,
            EU021218.1, AK001841.1 and AC015853.8.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_001128159.2.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and it encodes the longer protein (isoform 1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660805.155068.1,
                                           SRR1803617.254618.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000437048.7/ ENSP00000401435.2
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1                 AK092532.1         18-18
            2-217               AL701573.1         1-216
            218-415             EU021218.1         88-285
            416-502             AL701573.1         415-501
            503-1246            EU021218.1         373-1116
            1247-2215           AK001841.1         158-1126
            2216-2629           EU021218.1         2086-2499
            2630-13089          AC015853.8         15282-25741         c
FEATURES             Location/Qualifiers
     source          1..13089
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="17"
                     /map="17p13.3"
     gene            1..13089
                     /gene="VPS53"
                     /gene_synonym="HCCS1; hVps53L; PCH2E; pp13624"
                     /note="VPS53 subunit of GARP complex"
                     /db_xref="GeneID:55275"
                     /db_xref="HGNC:HGNC:25608"
                     /db_xref="MIM:615850"
     exon            1..217
                     /gene="VPS53"
                     /gene_synonym="HCCS1; hVps53L; PCH2E; pp13624"
                     /inference="alignment:Splign:2.1.0"
     CDS             131..2629
                     /gene="VPS53"
                     /gene_synonym="HCCS1; hVps53L; PCH2E; pp13624"
                     /note="isoform 1 is encoded by transcript variant 1;
                     vacuolar protein sorting-associated protein 53 homolog;
                     VPS53, GARP complex subunit; hepatocellular carcinoma
                     suppressor 1"
                     /codon_start=1
                     /product="vacuolar protein sorting-associated protein 53
                     homolog isoform 1"
                     /protein_id="NP_001121631.1"
                     /db_xref="CCDS:CCDS45558.1"
                     /db_xref="GeneID:55275"
                     /db_xref="HGNC:HGNC:25608"
                     /db_xref="MIM:615850"
                     /translation="MMEEEELEFVEELEAVLQLTPEVQLAIEQVFPSQDPLDRADFNA
                     VEYINTLFPTEQSLANIDEVVNKIRLKIRRLDDNIRTVVRGQTNVGQDGRQALEEAQK
                     AIQQLFGKIKDIKDKAEKSEQMVKEITRDIKQLDHAKRHLTTSITTLNHLHMLAGGVD
                     SLEAMTRRRQYGEVANLLQGVMNVLEHFHKYMGIPQIRQLSERVKAAQTELGQQILAD
                     FEEAFPSQGTKRPGGPSNVLRDACLVANILDPRIKQEIIKKFIKQHLSEYLVLFQENQ
                     DVAWLDKIDRRYAWIKRQLVDYEEKYGRMFPREWCMAERIAVEFCHVTRAELAKIMRT
                     RAKEIEVKLLLFAIQRTTNFEGFLAKRFSGCTLTDGTLKKLESPPPSTNPFLEDEPTP
                     EMEELATEKGDLDQPKKPKAPDNPFHGIVSKCFEPHLYVYIESQDKNLGELIDRFVAD
                     FKAQGPPKPNTDEGGAVLPSCADLFVYYKKCMVQCSQLSTGEPMIALTTIFQKYLREY
                     AWKILSGNLPKTTTSSGGLTISSLLKEKEGSEVAKFTLEELCLICNILSTAEYCLATT
                     QQLEEKLKEKVDVSLIERINLTGEMDTFSTVISSSIQLLVQDLDAACDPALTAMSKMQ
                     WQNVEHVGDQSPYVTSVILHIKQNVPIIRDNLASTRKYFTQFCVKFANSFIPKFITHL
                     FKCKPISMVGAEQLLLDTHSLKMVLLDLPSISSQVVRKAPASYTKIVVKGMTRAEMIL
                     KVVMAPHEPLVVFVDNYIKLLTDCNTETFQKILDMKGLKRSEQSSMLELLRQRLPAPP
                     SGAESSGSLSLTAPTPEQESSRIRKLEKLIKKRL"
     exon            218..298
                     /gene="VPS53"
                     /gene_synonym="HCCS1; hVps53L; PCH2E; pp13624"
                     /inference="alignment:Splign:2.1.0"
     exon            299..348
                     /gene="VPS53"
                     /gene_synonym="HCCS1; hVps53L; PCH2E; pp13624"
                     /inference="alignment:Splign:2.1.0"
     exon            349..415
                     /gene="VPS53"
                     /gene_synonym="HCCS1; hVps53L; PCH2E; pp13624"
                     /inference="alignment:Splign:2.1.0"
     exon            416..502
                     /gene="VPS53"
                     /gene_synonym="HCCS1; hVps53L; PCH2E; pp13624"
                     /inference="alignment:Splign:2.1.0"
     exon            503..618
                     /gene="VPS53"
                     /gene_synonym="HCCS1; hVps53L; PCH2E; pp13624"
                     /inference="alignment:Splign:2.1.0"
     exon            619..738
                     /gene="VPS53"
                     /gene_synonym="HCCS1; hVps53L; PCH2E; pp13624"
                     /inference="alignment:Splign:2.1.0"
     exon            739..817
                     /gene="VPS53"
                     /gene_synonym="HCCS1; hVps53L; PCH2E; pp13624"
                     /inference="alignment:Splign:2.1.0"
     exon            818..961
                     /gene="VPS53"
                     /gene_synonym="HCCS1; hVps53L; PCH2E; pp13624"
                     /inference="alignment:Splign:2.1.0"
     exon            962..1104
                     /gene="VPS53"
                     /gene_synonym="HCCS1; hVps53L; PCH2E; pp13624"
                     /inference="alignment:Splign:2.1.0"
     exon            1105..1246
                     /gene="VPS53"
                     /gene_synonym="HCCS1; hVps53L; PCH2E; pp13624"
                     /inference="alignment:Splign:2.1.0"
     exon            1247..1348
                     /gene="VPS53"
                     /gene_synonym="HCCS1; hVps53L; PCH2E; pp13624"
                     /inference="alignment:Splign:2.1.0"
     exon            1349..1443
                     /gene="VPS53"
                     /gene_synonym="HCCS1; hVps53L; PCH2E; pp13624"
                     /inference="alignment:Splign:2.1.0"
     exon            1444..1686
                     /gene="VPS53"
                     /gene_synonym="HCCS1; hVps53L; PCH2E; pp13624"
                     /inference="alignment:Splign:2.1.0"
     exon            1687..1834
                     /gene="VPS53"
                     /gene_synonym="HCCS1; hVps53L; PCH2E; pp13624"
                     /inference="alignment:Splign:2.1.0"
     exon            1835..1917
                     /gene="VPS53"
                     /gene_synonym="HCCS1; hVps53L; PCH2E; pp13624"
                     /inference="alignment:Splign:2.1.0"
     exon            1918..1996
                     /gene="VPS53"
                     /gene_synonym="HCCS1; hVps53L; PCH2E; pp13624"
                     /inference="alignment:Splign:2.1.0"
     exon            1997..2145
                     /gene="VPS53"
                     /gene_synonym="HCCS1; hVps53L; PCH2E; pp13624"
                     /inference="alignment:Splign:2.1.0"
     exon            2146..2215
                     /gene="VPS53"
                     /gene_synonym="HCCS1; hVps53L; PCH2E; pp13624"
                     /inference="alignment:Splign:2.1.0"
     exon            2216..2353
                     /gene="VPS53"
                     /gene_synonym="HCCS1; hVps53L; PCH2E; pp13624"
                     /inference="alignment:Splign:2.1.0"
     exon            2354..2458
                     /gene="VPS53"
                     /gene_synonym="HCCS1; hVps53L; PCH2E; pp13624"
                     /inference="alignment:Splign:2.1.0"
     exon            2459..13089
                     /gene="VPS53"
                     /gene_synonym="HCCS1; hVps53L; PCH2E; pp13624"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      5026
                     /gene="VPS53"
                     /gene_synonym="HCCS1; hVps53L; PCH2E; pp13624"
                     /note="major polyA site"
     regulatory      6666..6671
                     /regulatory_class="polyA_signal_sequence"
                     /gene="VPS53"
                     /gene_synonym="HCCS1; hVps53L; PCH2E; pp13624"
                     /note="hexamer: AATAAA"
     regulatory      6692..6697
                     /regulatory_class="polyA_signal_sequence"
                     /gene="VPS53"
                     /gene_synonym="HCCS1; hVps53L; PCH2E; pp13624"
                     /note="hexamer: AATAAA"
     polyA_site      6693
                     /gene="VPS53"
                     /gene_synonym="HCCS1; hVps53L; PCH2E; pp13624"
                     /note="major polyA site"
     polyA_site      6715
                     /gene="VPS53"
                     /gene_synonym="HCCS1; hVps53L; PCH2E; pp13624"
                     /note="major polyA site"
     regulatory      8514..8519
                     /regulatory_class="polyA_signal_sequence"
                     /gene="VPS53"
                     /gene_synonym="HCCS1; hVps53L; PCH2E; pp13624"
                     /note="hexamer: ATTAAA"
     polyA_site      8535
                     /gene="VPS53"
                     /gene_synonym="HCCS1; hVps53L; PCH2E; pp13624"
     regulatory      10245..10250
                     /regulatory_class="polyA_signal_sequence"
                     /gene="VPS53"
                     /gene_synonym="HCCS1; hVps53L; PCH2E; pp13624"
                     /note="hexamer: AATAAA"
     polyA_site      10262
                     /gene="VPS53"
                     /gene_synonym="HCCS1; hVps53L; PCH2E; pp13624"
     regulatory      13067..13072
                     /regulatory_class="polyA_signal_sequence"
                     /gene="VPS53"
                     /gene_synonym="HCCS1; hVps53L; PCH2E; pp13624"
                     /note="hexamer: ATTAAA"
     polyA_site      13089
                     /gene="VPS53"
                     /gene_synonym="HCCS1; hVps53L; PCH2E; pp13624"
ORIGIN      
        1 gacagagcgg ctgacggagc cgggctcacc aggtcgctgc cgcgagggag ttgctgtgct
       61 ggggcctggg tggcggctgg aggcctgagt tgggctcgcg gcgggggtcg gcagggggcc
      121 gggtggcgga atgatggagg aggaggaact ggagttcgtg gaggagctgg aagccgtgct
      181 gcagctcacg cccgaggtgc agctggccat cgagcaggtg tttccaagcc aggaccctct
      241 agatcgagca gatttcaatg ctgttgagta tatcaatacc ctgttcccaa ccgagcaatc
      301 tctggcgaac atagacgaag tcgtgaacaa aattaggctg aaaataagga gactggatga
      361 caatattcga actgttgtaa gaggtcagac gaacgtgggg caggatggac ggcaagcgct
      421 tgaagaggct cagaaagcta tccaacaact ctttggcaaa atcaaagata tcaaagacaa
      481 agctgaaaaa tcagagcaaa tggtgaaaga aatcacccgt gatattaagc aattagatca
      541 cgccaaacgc cacctgacca cctcaatcac cacactgaac cacctgcaca tgctggcagg
      601 aggtgtcgac tccctcgaag ccatgaccag gcgaagacaa tacggagaag ttgctaatct
      661 ccttcagggt gtgatgaatg tcctggagca cttccacaag tatatgggga ttccgcagat
      721 ccggcagctt tccgaaagag tgaaggctgc acagactgag ttaggacagc aaatcctggc
      781 agattttgaa gaagcgtttc cttcccaggg caccaagaga ccaggaggac ccagcaatgt
      841 tctacgagat gcatgtctgg ttgctaatat tctagatccc aggatcaaac aggaaatcat
      901 caaaaagttt attaaacagc atctgtcaga gtatctggta ctttttcaag aaaaccaaga
      961 tgttgcctgg ctggacaaaa tcgacagacg ctatgcctgg ataaaacgcc agcttgtgga
     1021 ctatgaggag aaatacggcc gcatgtttcc acgtgagtgg tgcatggctg agaggattgc
     1081 ggtggaattt tgccatgtga caagggcaga acttgccaag attatgcgta ccagagcgaa
     1141 ggaaattgaa gtgaaattgc ttctttttgc tattcaaaga acaactaact ttgaggggtt
     1201 tcttgcaaaa cgcttctccg gctgcaccct gaccgatggg accctgaaaa agcttgagtc
     1261 tccaccccca tctaccaatc ccttcctgga agatgagcca acaccagaga tggaggaact
     1321 ggcaacggag aaaggagatt tagatcaacc aaagaagcct aaagccccag acaatccatt
     1381 tcatggcatt gtttccaagt gttttgagcc tcatctctac gtgtatatcg aatcccaaga
     1441 caagaacctc ggagagctga tagatcggtt tgtggctgat ttcaaagccc aggggccacc
     1501 taagcccaac actgatgaag ggggtgccgt gctccccagc tgcgccgacc tctttgtcta
     1561 ctacaagaag tgcatggtgc aatgctctca gctcagtact ggggagccca tgatcgccct
     1621 gaccaccatt ttccagaagt acctccgaga atacgcctgg aaaatcctct ctggcaacct
     1681 gcccaaaacc acaaccagca gtggaggact gactatcagc agcctcctca aggaaaagga
     1741 gggctcagaa gtagccaagt tcactctgga ggagctctgc ctcatctgta acatcctgag
     1801 cacggcagag tactgtctgg ccaccaccca gcagctagaa gaaaaactca aagaaaaagt
     1861 ggatgtaagt ctgattgaac gaatcaatct gactggagag atggacacgt tcagcaccgt
     1921 catctccagc agtattcagc tgctggttca ggatctggat gctgcctgtg atcctgccct
     1981 gactgccatg agcaagatgc agtggcagaa cgtggagcac gttggtgacc agagccccta
     2041 cgtcacctct gtcattctgc acatcaagca gaacgtcccc atcatccgtg acaacctggc
     2101 ttccacacgc aagtacttca ctcagttctg cgttaaattt gcaaactcct tcattcccaa
     2161 attcatcacc cacctcttca agtgcaagcc aattagcatg gtgggagcag aacagctgct
     2221 gctggacacc cactcgctga agatggtcct gctcgatctc ccctccatca gctcgcaggt
     2281 ggtgaggaag gcacccgcca gctacaccaa gatcgttgtc aaaggcatga cccgggctga
     2341 gatgatcctc aaggtagtga tggcccctca tgaaccgttg gtggtgtttg ttgacaacta
     2401 catcaaactt ctcacagact gcaacacaga aacctttcag aagatactgg acatgaaggg
     2461 gctgaagagg agtgagcaga gcagcatgct ggaactcctg cgccagcggc tccccgcacc
     2521 gccctcgggg gcagaaagct ccggctcact gtccctgacg gcgccaacac cagagcaaga
     2581 gtcgtcacgc atccgcaagc tcgagaaact cattaaaaag agactgtagc agcagcaagg
     2641 ggccctttgc tcctggctgg agaccctcag cgcccgttcc ccagaagccc ccaacctctc
     2701 ctgtgctccc cggcactctc acatcgtcgg tcttcaaact tcctgggaca tgtgggttgt
     2761 tactgagtct ctcccatgcc ctgtcttact tcctgcccta atcggagacg ctgagtaagg
     2821 gctgggctct aagagggcga tttaggtgat ctctggttcg tgaagcagag gcagcagtgg
     2881 aggataggtc cctgagctgc ccttgactca tgtagcctcc ccatggcagt cacaccctct
     2941 tgacacgtgg gccccacccg tccttttttt tttttttttt tttttttttt gagacagagt
     3001 ctcgctctgt cacccaggct ggagtgcagt ggcgtgatct cggctcactg caacctccgc
     3061 ctcccaggtt caagcaattc tcctgcctca gcctcctgag tatcggatta caggcgtgag
     3121 ccaccgagcc cagccccttc ctccatttct ttactcccac cctgctcctg gtttctaggg
     3181 aagggaaacc aggaagttca cttggaagac gtttcctagg ttttctcatc agatgaagcc
     3241 atgggaacgc ccaggaagtc catactctca aacccctttc tgcccactca ccttcaggtc
     3301 cacagcactt tccagcgtca tctgaaatag caaaggtgat gtgcagcatc ccagtgatgt
     3361 gttcctgtgt tatgagaagg agtctggggt tctggatgcc tcaggaacag aatatggacc
     3421 ttaaagacca ggctgagagc ctggcctggc acgcagcctg cccgcccccc ccgcccttcc
     3481 tgtccaccgc gggctctctg cagcagggct tgccgaagag actgctcccg acctctcatg
     3541 tcatcctgca tccagagaat ggcagcatat caagccgtta accctgattc actcggttcc
     3601 cttgaaacca ggcctcagtt aaccatgacc acagctacag gcaaaccgct gctgttgctg
     3661 cctgcttttg ctgaagaatg atatcttgcc aggcacggcg gctcatgcct gtagtcgcaa
     3721 cactttggga gaccaaggca ggaggatcac ttgagtccag gagtttgaga ccagcctggg
     3781 caacatacag atgtctacaa aaataaaaat ttgcaaggtg tggtggcatg cacctgtagt
     3841 cccaactgct agggaggctg aggtgggcag attgcttgag ctcaggagtt tgagaccagt
     3901 ctgggcaacc tggcaaaacc aaaaatacaa aaaattggct gggtgtggtg gctcatgcct
     3961 gtaatcacag cactttggga ggctgaggca ggtggatcac ctgaggtcag gaattcgaga
     4021 ccagcctgcc caacatggcg aaaccctgtc tcaactaaaa ataaaaaaaa ttagccgggt
     4081 gtcatggcga gtgcctgtaa tcccagctac tggggaggct gaggcaggag aatcacttaa
     4141 acccgggagg tggaggctgc agtgagccaa gatttcacca ctgcactcca gcctgggtga
     4201 cagagcaaga ccccttctca aaaagttttc aggatagagg aaatttcctt aaaatccata
     4261 aaacttgtaa cagccgtaca gtaggaataa ggtttggaaa tctgcctgga aacctgattt
     4321 cctcatcccc accttgtttc ccaacataac cactgctctg tgcccctgcc ctctctgcca
     4381 ggctttctcc ccgatgccgt ccagctctga tcctgagtgt catttcttca tcggcttcca
     4441 ggctgtcctt gctagctggt ttgactctca cgcatcaagc gtctcaaatt tgagaatgcg
     4501 gaggagaaaa gaagtgttgc gagaaggaga tgcggccttc agttgaggtc tattaggaaa
     4561 agattccaaa taagacaagg gcggggggcg gcagtcggga gagcccccag gaagccctgt
     4621 agatgccccc accccagccc atggagttgc tatggttaag cagcctgagc cgtacagttg
     4681 aaaaggggcg gtggggccca tctccaaata gcacagccag ttcagccgtc tgtcctcaac
     4741 ccaggagagc tgggaaagcc agtggactcc tgcacctggt tcttgtcact cctggcgttt
     4801 gtgtacagat caccacttct ctttttggtt cattgttgag gagtcgtttt ccggacggct
     4861 ccatcacagc attgagctat caagctgctg ttttccacag ttggtggtca cagccaggga
     4921 agttgctgtc ttgatttgtt acattgtcag taatccccaa ggaaccaatg gatgtcagta
     4981 ggagtttctg ttaaatgtct ccttgatggg gactcagtac tgtgtagaga cgctgtgttt
     5041 ctcttctggg ggtgtgcatc agaaccactg gggcctttta aaatctacag atgccggccg
     5101 ggcgccgtgg ctcacgcctg gaatcccagc acttggggag gctgaggcgg gcggatcaca
     5161 agcgcaggaa attgagacca tccttgccaa tatggtgaaa ccccatctct acaaaaaata
     5221 caaaaattac cggggtgtgg tggcgtgcac acctcccagc tacttgggag gctgaggcag
     5281 gagaatcgct tgaacccggg aggcaaagat tgcagtgagc cgagatcacg ccactgcact
     5341 ccagcctggc gacagagtga gactctatct caaaacaaac aaaaaaacag atgcccggag
     5401 attttgagtc agtaagtttg gatggggcct ggcatacagg tttaaaagtt ccataggtag
     5461 ttataacgtt tacaccttgt taagaaccac tgcaacagag agatttgctt tatggaaggg
     5521 ctcctgccac gcattttcat acatgtctat ttttctgact ctaagctaaa agtgcgccga
     5581 cactacactt gagctggtgc caacgtggtg aagattctct actgataaca gctttcattt
     5641 taaacttgtg ttgggtcagg cgcggtggct cacgcctgta atcccagcac tttgggaggc
     5701 cgaggtgggc ggatcacatg aggtcacgag tttgagacca gcctggccaa catggcaaaa
     5761 tcccatctct actaaaaata caaaaaaaaa aaaaaaaaaa gcaggcaggt ggcgggtgcc
     5821 tgtaatccca gctacttggg aggctgaggc aggggaatca gttgaatccg ggaggcggag
     5881 gttgcagtga gccgaggtcc cgccactgca ctccagcctg ggagacagag tgagactcag
     5941 ttaaaaaaaa aagacttttg gtggtggtgg ggaccatgcc caggattggg ctctctccat
     6001 actgatgagg attcttcttg ttttgatgac caacccccaa gcagaagctt tatttattaa
     6061 catcacttca aaaataacgt atcaaagggg caccctaggc cgggtactgt ggcatcacac
     6121 ctgtaatgcc agtgctttga gaggctgagg tgggaagatt gcttgagccc aggacttcaa
     6181 gacaagcctg ggcaacagag tgagacccca tctctattaa aaaataggcc gggcatggtg
     6241 gctcacactg gtaatcccag cactttggga ggccgaggca ggtggatcat gtgaggtcag
     6301 gagttcgaga ccagcctggc cgacatggtg aaaccctgtc tttactaaaa atacaaaatt
     6361 agccaggcgt ggtggtgggc gcctgtaatc ccagctactc aggaggctga agcaggagga
     6421 tcgcttgaac ccaggaggca gaggctgcag tgagccgaga tcatgccatt gcactcctgc
     6481 ctgggtgacg gagcaagact ctctaaaaaa taataaggta aaaggagtac ctgtggatga
     6541 aagcagcagc taacagctct gtgattcagc tagacctaga tattgtgccc attccttccc
     6601 ctcagtccat tttctgtagc ccctttaaaa accaaaaaac aactttgaag tcactttggg
     6661 tttaaaataa atagtggtaa tttgtataat caataaagag ttcactctgc tcttactgag
     6721 gggacttgtc tgaaagctgg cttagcggtg tccaggccag agaacatcct gtgtggactc
     6781 ttaacaatga aacaggagcc gcaatggaca aggtgttctg atctccaaac ccagctcagg
     6841 gattgaagct aactaccgcc actccttcct ctctcgaaga acaaagagcc agtacccagg
     6901 gctctgccca cccccgagaa ggggtgcctg atttcttatg tctagggtgg ctcctctgtg
     6961 cagatcagac aggactgaga gcagacaagc tgagaccagc atgaccagta cagggcacgc
     7021 agagcgtggg ggcaggtccg aggctgctgc tcgatgcagg gtatgacacc aacccttcct
     7081 gaattgccat cggatgagga gacctggttc cctgttgaaa gaatgccagg ggaaatgctg
     7141 catgtgcaca gctggaaatg gggttccttc tctaggaaga gcactcggaa taacctgctg
     7201 gaaatggggt tccttcccta ggaagagcac tcagaataac ctgctggaaa tgggattcct
     7261 tggctaggaa gagcactcag aataacctgc tggaaatggg attccttggc taggaagagc
     7321 actcagaata acctgctgga aatgggattc cttacctagg aagagcactc ggaataacct
     7381 gctggaaatg ggattcctta cctaggaaga gcactcggaa taacctgctg gaaatggggt
     7441 tccttcccta ggaagagcac tcggaataac ctgctggaaa tggggttcct tccctaggaa
     7501 gagcactcgg aataacctgc tggaaatggg gttccttcgc taggaagagc actcggaata
     7561 acctgctgga aatgggattc cttcgctagg aagagcactc ggaataacct gctggaaatg
     7621 ggattccttc gctaggaaga gcactcggaa taacctgctg gaaatgagat tccttcccta
     7681 ggaagagcac tcggaataac ctgctggaaa tgggattcct tcgctaggaa gagcactcgg
     7741 aataacctgc tggaaatgag attccttcgc taggaagagc actcggaata acctgctgga
     7801 aatgggattc cttcgctagg aagagcactc ggaataacct gctggaaatg agattccttc
     7861 gctaggaaga gcactcggaa taacctgctg gaaatgggat tccttcgcta ggaagagcac
     7921 tcggaataac ctgctggaaa tgggattcct tcgctaggaa gagcactcgg aataacctgc
     7981 tggaaatggg attccttccc taggaagagc actcggaata acctgctgga aatgagattc
     8041 cttccctagg aagagcactc ggaataacct gctggaaatg ggattccttc gctaggaaga
     8101 gcactcggaa taacctgctg gaaatgggat tccttcgcta ggaagagcac tcagaacaac
     8161 ctgcctgctt gagggtgaca gcaagtgtct cacacttctc gtctactcca agtcagattc
     8221 ctaaaaataa aatagagctc caatgggcaa tttaagcaaa agccttagaa acatggacac
     8281 ctgaaatgct ggcctacatg aagggtacga tgacatttta tatattaaat aaaaattccc
     8341 agtcctgttt tagtattcat cagaatagcc tcttggaccc tcgaagttct acacctttgg
     8401 gagatataaa tttccttaaa ataaagggat tgcttctttg tgtaaatagc tttcattttc
     8461 tccatctgga agtgatttct gcctgcatgt tggagcacaa aatactgcag ttaattaaag
     8521 cattactgga tgtcatttgc tagaatgtat ttccttactg ctgacagaaa acagaacaga
     8581 ctctaggaaa gaagtagcaa gtccgtgacc ctggccgctc ctggtggctg cccttctccc
     8641 ttccctgaca gtctaaggaa gcaacgcgtg ggagcagaat gtggccgact gagcccagtc
     8701 ctgacctgcc cagttgcagt ggagcctgtc tgtaatgttg tgtaaagtgt tttgcttctg
     8761 acttggaacc ctacttgccc tgtgaatttc tatcctgtga accttccctt ctctgtcctt
     8821 accgttccca tcacagcctg ctcttccagt gacggccaag tgatttctct cctgcacgca
     8881 tgagtccatc ctgtttcctt agtagctgga tggacgtttt gccatgaaaa ctctttggga
     8941 agtcttgtgt cctttagtga tttctctcct gcacgcatga gtccatcctg tttccttagt
     9001 agctggttgg acgtttttgc catgaaaact cttcgggaag tcctgtgtcc tttcaacagg
     9061 ggaaaactgg ctgcggccct tccattaggc tccaggatca ttacctcgtt tccattcttt
     9121 cttttcaaca gctccccact ttactaggga agccaaggga tcaacctaat gagaaaagaa
     9181 ccttccctgt agcccacctg gaagggcatt gcctgccagc tccttccttc tcacctatgt
     9241 gaacaggagg gaagatgccg gcgatccatg gatctgtggg acatgaagga cgtgtccatc
     9301 agaaatgaaa gcagcctggg attggaagca tccacagaag acttgatctg caggtcttga
     9361 tttggacctg gcactttgac accaggcgag tcactgatcc cctgagcatc cattttcaat
     9421 attggtgaga ggggattata atgaatattg gctgtgaagg tcacacaaga ccgggctata
     9481 tgcacttgct ctgccataag gcagtgtgaa gtgtgttcgt gatcacttgt gggcgggagc
     9541 ctgtggctgt cttgcctgga ctggcttttt taaacatgga tgagtggtga caaatcctgc
     9601 ggccgtgtgc aggctacatc cgcagcgagc tccgcatatc tggtctttgc ctttttaggt
     9661 catgactgta atatcaagct tacacgagag ttttaaaaga ctgagcagta tccccttccc
     9721 ctggcagctg ctgcttccgt agcatttagg gataaacctg cagacactga gaagagagct
     9781 tcctccctcc tggggctggt ggcgaggctg cctgttgtgg aaaatagggc atgtctcctg
     9841 tgaatgcgaa ttgcgagggt tgccaaagcc tcccttccaa gaaacttgct taggtgtctg
     9901 gtgtccacag accagactct tctcccgaca gggacaaaaa ttcttgcctg cctttctttt
     9961 tctttttttt ccttttactt attcttgcct tttttcttct tcttcttctt taggtatgat
    10021 gccccccaaa aaactaagca agattaactt gggacaggca gttctaactg ggaagcctga
    10081 gctcacaggc tgtttggatt actttccaat tgtgtttcaa agatgtttag acttttccca
    10141 aggccagctg aatccttata atcagcccaa aagttccttt ctgtttctgt ctgtcccctt
    10201 tgagggtgtt gactcagaga acctggggca atatgagatg ttaaaataaa aatagcgtcc
    10261 aacgtcccgc ctcctgggaa tgctgctcac ttcatccgag ggttctggga gcttctgctc
    10321 agtcatcaaa tggaatttgc acaattgtct tgcctcctgt tttgggtaat ttttcaacaa
    10381 gtgagaaatt aagaacagct tctcaacaaa cttccctcta cttccagggc caagaaaagt
    10441 atttgcagtg tgggctgcag ctttcagggg tctcaggtga aatggtggca agaaacccca
    10501 gctcccttca tgtgtttgcc ttgcgctgat ggaaaaggaa ggcacttctc agctgccaga
    10561 gaccacattc cacagggttc ttcacaaaat caagcactgc agtggaccaa ctggagcagc
    10621 cactggaggg ctttagaagg caaatggaat acatatcaaa tgctatataa agaaaacatc
    10681 tgtttacaca gagtccccca cagcctcata cctacaccca atatcaaatg ctgtataaag
    10741 aaacatcagt ttacacacag agccccccac aacctcatac ccacaccgaa tatcaaatgc
    10801 tatacaaaga aacatcagtt tacacacaga gtcccccaca gcctcatacc catgcctgtc
    10861 acatctttgc acagccctaa aggatccaga gctttggatc agcagctggc tagctcggag
    10921 gccagaggag ggtgctgagg ggagtggagg tcagttgggg tagccctggg ccctggctgc
    10981 acagtccagc tgctgtgtgg cctgcatgtg gtatcttagg tcatgagagg gcctgggcaa
    11041 ttcagtgcta tctgtacatg ttggtggggt gggaaggaaa aggtaactgt gtatttactg
    11101 tgtcctggat acattatctc atcggatgaa attcctgcta agttaagctc cgtttagtgg
    11161 acgggacttg gaggctcaaa gagggtaagt gacaggccca aggatatgtg cctagtgagg
    11221 ggtgagccag gattcgatgt gtgactagta aggggtcagc caggattcaa tatgtgacta
    11281 gtaaggggtc agccaggatt tcatatggga ctagtaaggg gtcggccagg atttgatacg
    11341 tgactagcaa ggggacaagc caggatttga tatgtgacta gtgaggggcg agccaggatt
    11401 cgatatgtga ctagtaaggg gtcagccaag atttgatatg tgactagtga ggggcgagac
    11461 aggatttgat atgtgactag tgaggggcga gccaggattt gatatgtgac tggtgagggg
    11521 cgagccagga tttgatatgt gactggtgag gggcgagcca ggatttgata tgtgactggt
    11581 gaggggcgag ccaggatttg atatgtgact ggtgaggggc gagccaggat ttgatatgtg
    11641 actggtgagg ggcgagccag gatttgatat gtgactggtg aggggcgagc caggatttga
    11701 tatgtgactg gtgaggggcg agccaggatt tgatatgtga ctggtgaggg gcgagccagg
    11761 atttgatatg tgactggtga ggggcgagcc aggatttgat atgtgactgg tgaggggcga
    11821 gccaggattt gatatgtgac tggtgagggg cgagccagga tttgatatgt gactggtgag
    11881 gggtggagcc agtcaggatt tgatatgtgg ttagtgaggg gttagccagt caggatttga
    11941 tatgtggcta gtgaggggcg agccaggatt tgatatgtga ctagtaaggg ggtgagccag
    12001 gattcaatac gtgacaagtg aggggtgagc caggattcaa tacgtgacca gtgaggggcg
    12061 agccaggatt cgatacgtga ccagtgaggg gcgagccagg atgcgatacg tgaccagtga
    12121 ggggcgagcc aggattccat acgtgactag tgaggggtga gccaggattc gatatgtgag
    12181 tagtgaggag tgagccagga tttgatatgt ggctactgag gggcgagcca ggatttgata
    12241 tgtgactagt aaggggtcag ccaggatttg atacgtgact agtcaggggg tcagccagga
    12301 ttcgatacgt gactagtaag gggtcagcca ggatttgata tgtgactagt gaggggtgag
    12361 ccaggatttg acatgtgact agtgaggggt gagccaggat tcaatatgtg actagtgagg
    12421 ggtgagccag gattcgatat gtacctagtg aggggtcagc caggatttga tatgtggcta
    12481 gtaaggggtg agccagccag gatttgatat gtgactagtg acgagtgagc caggatttga
    12541 tatgtgacta gtaagggggt gaggcaggat ttgatatgtg actagtaagg gggtgaggca
    12601 ggatttgata tgtgactggt gaggggtgag acaggatttg atatgtgact agtgaggggt
    12661 gagccaggat ttgctatgtg actagtaagg gggcgagcca ggatttggct ccagccttca
    12721 ttccaacatc catgcctttt gtacttcacc agcagttttc aaatttatgg gacaaaattt
    12781 tacattggat tccctctata caaaatagat aaaagtattt tatgaataca ttttatgagt
    12841 tcaaatattt ttcagaagaa atcagtaaaa gcattgaaaa ctattccagc aaataggaag
    12901 cactttaaac caaggttatg gatgaaaatg tgtgtgtatc aaccccagtt tacttctggg
    12961 ttgcacagaa tagagaaatc ttgatttaat ccagctaaga attttcaaat agtatccatt
    13021 ttgttttgtt ttaaacagct cttcttgcaa tggtagaggc tcttcaatta aacagagatg
    13081 gtaacaggc
//
