LOCUS       NM_017667               5684 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens VPS50 subunit of EARP/GARPII complex (VPS50),
            transcript variant 1, mRNA.
ACCESSION   NM_017667
VERSION     NM_017667.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5684)
  AUTHORS   Schneeberger,P.E., Nampoothiri,S., Holling,T., Yesodharan,D.,
            Alawi,M., Knisely,A.S., Muller,T., Plecko,B., Janecke,A.R. and
            Kutsche,K.
  TITLE     Biallelic variants in VPS50 cause a neurodevelopmental disorder
            with neonatal cholestasis
  JOURNAL   Brain 144 (10), 3036-3049 (2021)
   PUBMED   34037727
  REMARK    GeneRIF: Biallelic variants in VPS50 cause a neurodevelopmental
            disorder with neonatal cholestasis.
REFERENCE   2  (bases 1 to 5684)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   3  (bases 1 to 5684)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Paulo,J.A., Cannon,J.R., Ting,L.,
            Baltier,K., Colby,G., Gebreab,F., Gygi,M.P., Parzen,H., Szpyt,J.,
            Tam,S., Zarraga,G., Pontano-Vaites,L., Swarup,S., White,A.E.,
            Schweppe,D.K., Rad,R., Erickson,B.K., Obar,R.A., Guruharsha,K.G.,
            Li,K., Artavanis-Tsakonas,S., Gygi,S.P. and Harper,J.W.
  TITLE     Architecture of the human interactome defines protein communities
            and disease networks
  JOURNAL   Nature 545 (7655), 505-509 (2017)
   PUBMED   28514442
REFERENCE   4  (bases 1 to 5684)
  AUTHORS   Hein,M.Y., Hubner,N.C., Poser,I., Cox,J., Nagaraj,N., Toyoda,Y.,
            Gak,I.A., Weisswange,I., Mansfeld,J., Buchholz,F., Hyman,A.A. and
            Mann,M.
  TITLE     A human interactome in three quantitative dimensions organized by
            stoichiometries and abundances
  JOURNAL   Cell 163 (3), 712-723 (2015)
   PUBMED   26496610
REFERENCE   5  (bases 1 to 5684)
  AUTHORS   Schindler,C., Chen,Y., Pu,J., Guo,X. and Bonifacino,J.S.
  TITLE     EARP is a multisubunit tethering complex involved in endocytic
            recycling
  JOURNAL   Nat Cell Biol 17 (5), 639-650 (2015)
   PUBMED   25799061
REFERENCE   6  (bases 1 to 5684)
  AUTHORS   Bailey,S.D., Xie,C., Do,R., Montpetit,A., Diaz,R., Mohan,V.,
            Keavney,B., Yusuf,S., Gerstein,H.C., Engert,J.C. and Anand,S.
  CONSRTM   DREAM investigators
  TITLE     Variation at the NFATC2 locus increases the risk of
            thiazolidinedione-induced edema in the Diabetes REduction
            Assessment with ramipril and rosiglitazone Medication (DREAM) study
  JOURNAL   Diabetes Care 33 (10), 2250-2253 (2010)
   PUBMED   20628086
  REMARK    GeneRIF: Observational study of gene-disease association,
            gene-environment interaction, and pharmacogenomic / toxicogenomic.
            (HuGE Navigator)
REFERENCE   7  (bases 1 to 5684)
  AUTHORS   Ghosh,D., Lippert,D., Krokhin,O., Cortens,J.P. and Wilkins,J.A.
  TITLE     Defining the membrane proteome of NK cells
  JOURNAL   J Mass Spectrom 45 (1), 1-25 (2010)
   PUBMED   19946888
REFERENCE   8  (bases 1 to 5684)
  AUTHORS   Talmud,P.J., Drenos,F., Shah,S., Shah,T., Palmen,J., Verzilli,C.,
            Gaunt,T.R., Pallas,J., Lovering,R., Li,K., Casas,J.P., Sofat,R.,
            Kumari,M., Rodriguez,S., Johnson,T., Newhouse,S.J., Dominiczak,A.,
            Samani,N.J., Caulfield,M., Sever,P., Stanton,A., Shields,D.C.,
            Padmanabhan,S., Melander,O., Hastie,C., Delles,C., Ebrahim,S.,
            Marmot,M.G., Smith,G.D., Lawlor,D.A., Munroe,P.B., Day,I.N.,
            Kivimaki,M., Whittaker,J., Humphries,S.E. and Hingorani,A.D.
  CONSRTM   ASCOT investigators; NORDIL investigators; BRIGHT Consortium
  TITLE     Gene-centric association signals for lipids and apolipoproteins
            identified via the HumanCVD BeadChip
  JOURNAL   Am J Hum Genet 85 (5), 628-642 (2009)
   PUBMED   19913121
  REMARK    GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
REFERENCE   9  (bases 1 to 5684)
  AUTHORS   Gonzales,P.A., Pisitkun,T., Hoffert,J.D., Tchapyjnikov,D.,
            Star,R.A., Kleta,R., Wang,N.S. and Knepper,M.A.
  TITLE     Large-scale proteomics and phosphoproteomics of urinary exosomes
  JOURNAL   J Am Soc Nephrol 20 (2), 363-379 (2009)
   PUBMED   19056867
REFERENCE   10 (bases 1 to 5684)
  AUTHORS   Canestri,F.
  TITLE     A proposed clinical application of a model of CO2 laser radiation
            induced damage craters
  JOURNAL   J Med Eng Technol 12 (3), 112-117 (1988)
   PUBMED   3172165
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BC017888.1, AB100163.1, BC035984.1, AC002379.1 and BE671517.1.
            
            On Nov 22, 2018 this sequence version replaced NM_017667.3.
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (a).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803611.122612.1,
                                           SRR1660805.255896.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000305866.10/ ENSP00000307666.5
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-17                BC017888.1         27-43
            18-2268             AB100163.1         1-2251
            2269-3587           BC035984.1         298-1616
            3588-5677           AC002379.1         86323-88412
            5678-5684           BE671517.1         4-10                c
FEATURES             Location/Qualifiers
     source          1..5684
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="7"
                     /map="7q21.2-q21.3"
     gene            1..5684
                     /gene="VPS50"
                     /gene_synonym="CCDC132; NEDMSC; VPS54L"
                     /note="VPS50 subunit of EARP/GARPII complex"
                     /db_xref="GeneID:55610"
                     /db_xref="HGNC:HGNC:25956"
                     /db_xref="MIM:616465"
     exon            1..135
                     /gene="VPS50"
                     /gene_synonym="CCDC132; NEDMSC; VPS54L"
                     /inference="alignment:Splign:2.1.0"
     CDS             103..2997
                     /gene="VPS50"
                     /gene_synonym="CCDC132; NEDMSC; VPS54L"
                     /note="isoform a is encoded by transcript variant 1;
                     coiled-coil domain-containing protein 132; syndetin;
                     coiled-coil domain containing 132; EARP/GARPII complex
                     subunit VPS50; VPS50, EARP/GARPII complex subunit"
                     /codon_start=1
                     /product="syndetin isoform a"
                     /protein_id="NP_060137.2"
                     /db_xref="CCDS:CCDS43617.1"
                     /db_xref="GeneID:55610"
                     /db_xref="HGNC:HGNC:25956"
                     /db_xref="MIM:616465"
                     /translation="MQKIKSLMTRQGLKSPQESLSDLGAIESLRVPGKEEFRELREQP
                     SDPQAEQELINSIEQVYFSVDSFDIVKYELEKLPPVLNLQELEAYRDKLKQQQAAVSK
                     KVADLILEKQPAYVKELERVTSLQTGLQLAAVICTNGRRHLNIAKEGFTQASLGLLAN
                     QRKRQLLIGLLKSLRTIKTLQRTDVRLSEMLEEEDYPGAIQLCLECQKAASTFKHYSC
                     ISELNSKLQDTLEQIEEQLDVALSKICKNFDINHYTKVQQAYRLLGKTQTAMDQLHMH
                     FTQAIHNTVFQVVLGYVELCAGNTDTKFQKLQYKDLCTHVTPDSYIPCLADLCKALWE
                     VMLSYYRTMEWHEKHDNEDTASASEGSNMIGTEETNFDRGYIKKKLEHGLTRIWQDVQ
                     LKVKTYLLGTDLSIFKYDDFIFVLDIISRLMQVGEEFCGSKSEVLQESIRKQSVNYFK
                     NYHRTRLDELRMFLENETWELCPVKSNFSILQLHEFKFMEQSRSPSVSPSKQPVSTSS
                     KTVTLFEQYCSGGNPFEIQANHKDEETEDVLASNGYESDEQEKSAYQEYDSDSDVPEE
                     LKRDYVDEQTGDGPVKSVSRETLKSRKKSDYSLNKVNAPILTNTTLNVIRLVGKYMQM
                     MNILKPIAFDVIHFMSQLFDYYLYAIYTFFGRNDSLESTGLGLSSSRLRTTLNRIQES
                     LIDLEVSADPTATLTAAEERKEKVPSPHLSHLVVLTSGDTLYGLAERVVATESLVFLA
                     EQFEFLQPHLDAVMPAVKKPFLQQFYSQTVSTASELRKPIYWIVAGKALDYEQMLLLM
                     ANVKWDVKEIMSQHNIYVDALLKEFEQFNRRLNEVSKRVRIPLPVSNILWEHCIRLAN
                     RTIVEGYANVKKCSNEGRALMQLDFQQFLMKLEKLTDIRPIPDKEFVETYIKAYYLTE
                     NDMERWIKEHREYSTKQLTNLVNVCLGSHINKKARQKLLAAIDDIDRPKR"
     exon            136..204
                     /gene="VPS50"
                     /gene_synonym="CCDC132; NEDMSC; VPS54L"
                     /inference="alignment:Splign:2.1.0"
     exon            205..327
                     /gene="VPS50"
                     /gene_synonym="CCDC132; NEDMSC; VPS54L"
                     /inference="alignment:Splign:2.1.0"
     exon            328..399
                     /gene="VPS50"
                     /gene_synonym="CCDC132; NEDMSC; VPS54L"
                     /inference="alignment:Splign:2.1.0"
     exon            400..453
                     /gene="VPS50"
                     /gene_synonym="CCDC132; NEDMSC; VPS54L"
                     /inference="alignment:Splign:2.1.0"
     exon            454..524
                     /gene="VPS50"
                     /gene_synonym="CCDC132; NEDMSC; VPS54L"
                     /inference="alignment:Splign:2.1.0"
     exon            525..642
                     /gene="VPS50"
                     /gene_synonym="CCDC132; NEDMSC; VPS54L"
                     /inference="alignment:Splign:2.1.0"
     exon            643..678
                     /gene="VPS50"
                     /gene_synonym="CCDC132; NEDMSC; VPS54L"
                     /inference="alignment:Splign:2.1.0"
     exon            679..761
                     /gene="VPS50"
                     /gene_synonym="CCDC132; NEDMSC; VPS54L"
                     /inference="alignment:Splign:2.1.0"
     exon            762..804
                     /gene="VPS50"
                     /gene_synonym="CCDC132; NEDMSC; VPS54L"
                     /inference="alignment:Splign:2.1.0"
     exon            805..903
                     /gene="VPS50"
                     /gene_synonym="CCDC132; NEDMSC; VPS54L"
                     /inference="alignment:Splign:2.1.0"
     exon            904..1044
                     /gene="VPS50"
                     /gene_synonym="CCDC132; NEDMSC; VPS54L"
                     /inference="alignment:Splign:2.1.0"
     exon            1045..1177
                     /gene="VPS50"
                     /gene_synonym="CCDC132; NEDMSC; VPS54L"
                     /inference="alignment:Splign:2.1.0"
     exon            1178..1269
                     /gene="VPS50"
                     /gene_synonym="CCDC132; NEDMSC; VPS54L"
                     /inference="alignment:Splign:2.1.0"
     exon            1270..1364
                     /gene="VPS50"
                     /gene_synonym="CCDC132; NEDMSC; VPS54L"
                     /inference="alignment:Splign:2.1.0"
     exon            1365..1463
                     /gene="VPS50"
                     /gene_synonym="CCDC132; NEDMSC; VPS54L"
                     /inference="alignment:Splign:2.1.0"
     exon            1464..1554
                     /gene="VPS50"
                     /gene_synonym="CCDC132; NEDMSC; VPS54L"
                     /inference="alignment:Splign:2.1.0"
     exon            1555..1731
                     /gene="VPS50"
                     /gene_synonym="CCDC132; NEDMSC; VPS54L"
                     /inference="alignment:Splign:2.1.0"
     exon            1732..1850
                     /gene="VPS50"
                     /gene_synonym="CCDC132; NEDMSC; VPS54L"
                     /inference="alignment:Splign:2.1.0"
     exon            1851..1957
                     /gene="VPS50"
                     /gene_synonym="CCDC132; NEDMSC; VPS54L"
                     /inference="alignment:Splign:2.1.0"
     exon            1958..2079
                     /gene="VPS50"
                     /gene_synonym="CCDC132; NEDMSC; VPS54L"
                     /inference="alignment:Splign:2.1.0"
     exon            2080..2160
                     /gene="VPS50"
                     /gene_synonym="CCDC132; NEDMSC; VPS54L"
                     /inference="alignment:Splign:2.1.0"
     exon            2161..2309
                     /gene="VPS50"
                     /gene_synonym="CCDC132; NEDMSC; VPS54L"
                     /inference="alignment:Splign:2.1.0"
     exon            2310..2406
                     /gene="VPS50"
                     /gene_synonym="CCDC132; NEDMSC; VPS54L"
                     /inference="alignment:Splign:2.1.0"
     exon            2407..2565
                     /gene="VPS50"
                     /gene_synonym="CCDC132; NEDMSC; VPS54L"
                     /inference="alignment:Splign:2.1.0"
     exon            2566..2687
                     /gene="VPS50"
                     /gene_synonym="CCDC132; NEDMSC; VPS54L"
                     /inference="alignment:Splign:2.1.0"
     exon            2688..2877
                     /gene="VPS50"
                     /gene_synonym="CCDC132; NEDMSC; VPS54L"
                     /inference="alignment:Splign:2.1.0"
     exon            2878..5684
                     /gene="VPS50"
                     /gene_synonym="CCDC132; NEDMSC; VPS54L"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3559..3564
                     /regulatory_class="polyA_signal_sequence"
                     /gene="VPS50"
                     /gene_synonym="CCDC132; NEDMSC; VPS54L"
                     /note="hexamer: AATAAA"
     polyA_site      3587
                     /gene="VPS50"
                     /gene_synonym="CCDC132; NEDMSC; VPS54L"
                     /note="major polyA site"
     regulatory      3845..3850
                     /regulatory_class="polyA_signal_sequence"
                     /gene="VPS50"
                     /gene_synonym="CCDC132; NEDMSC; VPS54L"
                     /note="hexamer: AGTAAA"
     polyA_site      3874
                     /gene="VPS50"
                     /gene_synonym="CCDC132; NEDMSC; VPS54L"
     regulatory      4070..4075
                     /regulatory_class="polyA_signal_sequence"
                     /gene="VPS50"
                     /gene_synonym="CCDC132; NEDMSC; VPS54L"
                     /note="hexamer: CATAAA"
     polyA_site      4092
                     /gene="VPS50"
                     /gene_synonym="CCDC132; NEDMSC; VPS54L"
     regulatory      5453..5458
                     /regulatory_class="polyA_signal_sequence"
                     /gene="VPS50"
                     /gene_synonym="CCDC132; NEDMSC; VPS54L"
                     /note="hexamer: AATAAA"
     polyA_site      5475
                     /gene="VPS50"
                     /gene_synonym="CCDC132; NEDMSC; VPS54L"
     regulatory      5479..5484
                     /regulatory_class="polyA_signal_sequence"
                     /gene="VPS50"
                     /gene_synonym="CCDC132; NEDMSC; VPS54L"
                     /note="hexamer: AATGAA"
     polyA_site      5496
                     /gene="VPS50"
                     /gene_synonym="CCDC132; NEDMSC; VPS54L"
     regulatory      5529..5534
                     /regulatory_class="polyA_signal_sequence"
                     /gene="VPS50"
                     /gene_synonym="CCDC132; NEDMSC; VPS54L"
                     /note="hexamer: AATAAA"
     polyA_site      5553
                     /gene="VPS50"
                     /gene_synonym="CCDC132; NEDMSC; VPS54L"
     regulatory      5615..5620
                     /regulatory_class="polyA_signal_sequence"
                     /gene="VPS50"
                     /gene_synonym="CCDC132; NEDMSC; VPS54L"
                     /note="hexamer: AGTAAA"
     polyA_site      5639
                     /gene="VPS50"
                     /gene_synonym="CCDC132; NEDMSC; VPS54L"
     regulatory      5665..5670
                     /regulatory_class="polyA_signal_sequence"
                     /gene="VPS50"
                     /gene_synonym="CCDC132; NEDMSC; VPS54L"
                     /note="hexamer: AATAAA"
     polyA_site      5684
                     /gene="VPS50"
                     /gene_synonym="CCDC132; NEDMSC; VPS54L"
ORIGIN      
        1 gtgaccaccc actatggctt cctagtgtca gggccagctg tgtagtggct cggtgtgatt
       61 tgttagctct ttgaggcagg gtaccctcct caggatttcg atatgcaaaa aatcaaatct
      121 ctcatgaccc gacagggtct gaaaagccct caagaaagcc tcagtgatct tggtgccata
      181 gagagtctcc gggtccctgg aaaggaagaa ttcagggaac ttcgagaaca gccaagtgac
      241 cctcaagctg aacaagagct tattaatagt attgaacaag tatatttttc tgtggattca
      301 tttgatattg ttaaatatga gctggagaag cttccacctg ttctcaattt gcaagaatta
      361 gaggcgtata gagacaaatt gaaacaacag caagctgcag tatctaaaaa agtggcagat
      421 ttaatccttg aaaaacagcc tgcttatgta aaggaacttg aaagagttac ctcattgcag
      481 acaggtcttc aattagctgc tgttatctgt acaaatggga gaagacactt gaatattgca
      541 aaggaaggtt ttactcaagc tagtttaggc cttcttgcaa atcaaaggaa acgtcagttg
      601 ctgattggac ttctgaaatc tctgagaact ataaaaacat tgcaaagaac agatgtacgg
      661 ttaagtgaaa tgctggagga ggaagattat ccaggagcta ttcagttgtg ccttgaatgt
      721 caaaaagctg ccagcacttt taaacattac agttgtataa gtgaactgaa ttcaaagctg
      781 caagatactt tggaacagat tgaggaacag ctggacgtag ctctttccaa aatctgcaag
      841 aattttgaca ttaaccatta taccaaggtt caacaagctt atcgacttct tggaaaaaca
      901 cagacagcaa tggatcaact tcatatgcac ttcacccaag ccattcacaa caccgtgttt
      961 caagttgttc ttggttatgt ggaactatgt gcaggaaaca cagacacaaa attccaaaag
     1021 ctgcaatata aggatctctg tacacatgtt acaccagaca gctatattcc atgccttgca
     1081 gacctgtgca aagcactatg ggaagttatg ctcagctatt ataggactat ggaatggcat
     1141 gaaaagcatg acaatgagga tactgcttca gcttctgaag ggagtaatat gataggtact
     1201 gaagaaacta attttgatcg tggctacata aaaaagaaat tagaacatgg acttacacga
     1261 atatggcagg atgttcagct aaaagtaaaa acctacttgc ttggaactga tttgtctata
     1321 ttcaaatatg atgatttcat ctttgttttg gatataatca gcaggttgat gcaagttgga
     1381 gaagaatttt gtggtagcaa gtctgaagtt ttacaggaat ctattagaaa acaaagtgtc
     1441 aattatttca agaattacca tagaacacgg ctcgatgaac tgagaatgtt cttagagaat
     1501 gagacttggg aactttgtcc tgttaagtca aatttcagca tcttgcaact tcatgaattt
     1561 aaattcatgg aacagtctcg ctccccatca gtttcaccta gtaaacagcc agtctcaact
     1621 tcttcaaaaa cagtgacctt gtttgagcag tactgtagtg gtgggaatcc atttgaaatt
     1681 caggccaacc acaaagatga agaaacagaa gatgtcttag cttctaatgg gtatgaatct
     1741 gatgaacaag aaaagagtgc ctatcaagag tatgacagtg acagtgatgt tcctgaggaa
     1801 ctcaaacgag actatgtgga tgagcagaca ggagatggtc ctgtgaaaag tgtttctcgg
     1861 gaaactctaa aaagcaggaa gaaatcagat tacagtctaa ataaagtgaa tgcacctatc
     1921 ttaacaaata caacattgaa cgtcataaga cttgttggaa aatatatgca gatgatgaac
     1981 attcttaagc caattgcctt tgatgttatt catttcatgt ctcaactatt tgattattac
     2041 ttgtatgcaa tatatacctt ttttggtcgg aatgattcat tggaatcaac tggactcggc
     2101 cttagtagta gtagactaag aacaactcta aacagaatac aagaaagcct tattgatcta
     2161 gaagtttcag ctgatcctac tgccacactc acagcagcag aagaaagaaa ggagaaggtg
     2221 ccaagtccac acctcagtca cctagtggtt ttgacatctg gggatacgct gtatgggttg
     2281 gcagaaagag tggtagccac ggaatccttg gtattcttgg ctgaacagtt tgagttcctt
     2341 cagccacatc tggatgctgt gatgcctgca gtcaaaaagc cctttcttca gcagttctat
     2401 tctcagacag tctcaaccgc cagtgaacta cggaaaccaa tttactggat tgtagctggt
     2461 aaagcccttg attatgaaca gatgctgctt ctcatggcta atgtgaaatg ggatgtaaaa
     2521 gaaattatgt cacagcacaa catatatgta gatgcactat taaaggaatt tgagcagttt
     2581 aacaggaggc taaatgaagt ttctaagaga gttcgcatac ccttgcctgt gtctaatata
     2641 ctttgggaac attgtatacg attggctaat cgaactattg tagaaggata tgccaatgtc
     2701 aagaaatgca gtaatgaggg tcgtgccctg atgcaattgg attttcaaca gtttttaatg
     2761 aaacttgaaa aactaacaga tattagaccc attcctgata aagaatttgt agaaacttat
     2821 attaaagctt attacctaac tgagaatgac atggaacggt ggatcaaaga gcacagggaa
     2881 tattcaacga agcagctgac caatctggtg aatgtttgcc tgggatccca tatcaataag
     2941 aaagcaagac aaaaacttct agcagctata gatgatatag acagacctaa aagataatga
     3001 acacagctct ctttcctcaa tggcattgat cctcactcaa catatatgac ctgaaagcca
     3061 gtttttttat gcacttctga caactatctg ctaagaaaac tttgtgcatg tttttttgac
     3121 tggaaagtgg aaaatattga aatgtgtgtg gtgttctcat gacttttata tgctgtggtc
     3181 tcttcaactt ttggtctcat ttgttgtaat ctgaaatgat gttgccgcct tgtcataaca
     3241 atggttatgt gactacagtt atacatttta cagaagaatg taccataagt atataattag
     3301 aagaacagtg gcttaatata tgtatgggaa gtttatggaa aatgaagttg gcacttttct
     3361 accctctgag cttggttctt aataagcata atgtgagggt gaatatgtag tatctcctaa
     3421 ttatgagcac tgcatgagaa ttaaaaaaca catgtaagta aaatagttga aaaatcagta
     3481 tgttctctgt ttttaaaatg tcaaagttta tgtcagggtt aatttagtta taacaaagtg
     3541 atcataatgg tgaaatttaa taaatatact ctagtatgat cagcctatgt gagactacat
     3601 tttgattttt tgtgtggcat gcagatgtca tgaactatag tgcagctttt agtgtgttct
     3661 aattttaatt gttttatatc ctgaaaccaa tggtgaaaag taatttcatt gagggtacct
     3721 tttcaatgcc tgagtagcat acagaatcat gattatgaga ctttctttta tctttcttta
     3781 taaaaatatg tggttttttt gttgaaagtt ttggtctctt taaattcaga ttttgtctta
     3841 ggacagtaaa acccaggttg actgactcag gaaacagttg tctgctagtc actcataaat
     3901 gtacggtcat atgttcactc ttcttaaata tccacctttt ataacacaaa tgtaaaatag
     3961 tatcagtcta gccaatgatg aactctggaa tccacttagt cttcagtaag tatgtgctgt
     4021 tcctctaaac tttgccctga agccagggat cttctcctaa tgtatgtgac ataaaaatcc
     4081 attttccatg tactcttgcc aactgtctga taaggaattt tgtgtgtgtt ctttctgact
     4141 ggagagtgga ggacatctaa gcttgtgtgg cattggtcca atgattgcca tgcatgacct
     4201 gtaacatcaa cagctcacag aatcctaagg aatcatgtga gaaacaggcc ttgggtctta
     4261 tgagtcttct gggacctaag gtgataaaat ggatggcaca gaatggcatg tgatcaagca
     4321 tggaggtgat aaggaaacaa ttgctactct gtgtccatca gtccctgcga tgtctgtaaa
     4381 tgcacagtgt aatgtgaagc actttcagaa tatgtttgtg tgtgtctgta tgctgagtgt
     4441 tttcttttca tcctctcatg tcggagttca tttactgtca atttaaaact ttcctcttgt
     4501 aaaaaggtat gagtttggtg ccattatgat ttggttgtgg ctagattata tacttgtgaa
     4561 gtagattctg cctgtagcca ttatttatat gcagccactt gaagaaaaca aacatgagat
     4621 aagcttggtc cttgagttta aatggcatat ttagctgggc acataaagag ctaatcaatc
     4681 ttttaaaatt tgcagttgat gaagagctgc ttatttttct cttctacatg gattttatag
     4741 ttggtttcct ttaatttcta ctttagttgg ccctagataa tatgaacagg aatcacctgc
     4801 ctggtagagc ccagtgtttt ccaaggtaat aggaattgat acaaaagaat tacttatctt
     4861 gttagagact cgaccatgca atctgacaga ccttttaacc tacccttact ttgctttctc
     4921 catcttcaaa atgaaatggt ggatcttttt aatgcttttt gaaaaaggtt tttttttttt
     4981 ttttttttca ttaaaaggta gcttaataag tgctagctag catcaaaaca gtaacaggag
     5041 gtttgagtca tgaagttacc taatcacaag agagttacca gacatttctc aaatacttta
     5101 tacagtaata ttgagttata accatgtaac attttatgaa actaattttc agataaatac
     5161 aattgaaaag ctgagaatgt aaatggaatt atacgtgttc caaaaactat cattaccaaa
     5221 aatttattat ttttgagtct aaactttcct taactttgac attttccatg aaagtttcct
     5281 gtagaattta ccatgggagc atggtaaatt agcattccct gagaaagaat gatcatttct
     5341 acaaatacat tgtcttgcat ttaaaaatgc taggggggca catagcaatt caaatgaact
     5401 agtttgcttt ggtttataaa accaacagag attttgtaag ttcactttaa ctaataaact
     5461 catttacaca acttattaaa tgaatttctc ttgaaaccag ggccatatgg tattttatca
     5521 tatctttaaa taaaatttcg aaatttctat ttagctgagt gctaaagtga aactttgtta
     5581 gattaaatag ctttttctta actcaccaaa aagtagtaaa aggcttgtaa ttcaattcac
     5641 cttgtaagca tagttgtctt ttgtaataaa aaactagatc tgaa
//
