LOCUS       NP_037377                437 aa            linear   PRI 28-APR-2025
DEFINITION  vacuolar protein sorting-associated protein 4A [Homo sapiens].
ACCESSION   NP_037377
VERSION     NP_037377.1
DBSOURCE    REFSEQ: accession NM_013245.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (residues 1 to 437)
  AUTHORS   Dvilansky,I., Altaras,Y., Kamenetsky,N., Nachmias,D. and Elia,N.
  TITLE     The human AAA-ATPase VPS4A isoform and its co-factor VTA1 have a
            unique function in regulating mammalian cytokinesis abscission
  JOURNAL   PLoS Biol 22 (4), e3002327 (2024)
   PUBMED   38687820
  REMARK    GeneRIF: The human AAA-ATPase VPS4A isoform and its co-factor VTA1
            have a unique function in regulating mammalian cytokinesis
            abscission.
            Publication Status: Online-Only
REFERENCE   2  (residues 1 to 437)
  AUTHORS   Yang,Y., Chen,H.L., Wu,S.F. and Bao,W.
  TITLE     CHMP4B and VSP4A reverse GSDMD-mediated pyroptosis by cell membrane
            remodeling in endometrial carcinoma
  JOURNAL   Biochim Biophys Acta Gen Subj 1868 (1), 130497 (2024)
   PUBMED   37931722
  REMARK    GeneRIF: CHMP4B and VSP4A reverse GSDMD-mediated pyroptosis by cell
            membrane remodeling in endometrial carcinoma.
REFERENCE   3  (residues 1 to 437)
  AUTHORS   Harel,S., Altaras,Y., Nachmias,D., Rotem-Dai,N., Dvilansky,I.,
            Elia,N. and Rousso,I.
  TITLE     Analysis of individual HIV-1 budding event using fast AFM reveals a
            multiplexed role for VPS4
  JOURNAL   Biophys J 121 (21), 4229-4238 (2022)
   PUBMED   36042696
  REMARK    GeneRIF: Analysis of individual HIV-1 budding event using fast AFM
            reveals a multiplexed role for VPS4.
REFERENCE   4  (residues 1 to 437)
  AUTHORS   Chevalier,B., Baatallah,N., Najm,M., Castanier,S., Jung,V.,
            Pranke,I., Golec,A., Stoven,V., Marullo,S., Antigny,F.,
            Guerrera,I.C., Sermet-Gaudelus,I., Edelman,A. and Hinzpeter,A.
  TITLE     Differential CFTR-Interactome Proximity Labeling Procedures
            Identify Enrichment in Multiple SLC Transporters
  JOURNAL   Int J Mol Sci 23 (16), 8937 (2022)
   PUBMED   36012204
  REMARK    Publication Status: Online-Only
REFERENCE   5  (residues 1 to 437)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   6  (residues 1 to 437)
  AUTHORS   Howard,T.L., Stauffer,D.R., Degnin,C.R. and Hollenberg,S.M.
  TITLE     CHMP1 functions as a member of a newly defined family of vesicle
            trafficking proteins
  JOURNAL   J Cell Sci 114 (Pt 13), 2395-2404 (2001)
   PUBMED   11559748
REFERENCE   7  (residues 1 to 437)
  AUTHORS   Hu,R.M., Han,Z.G., Song,H.D., Peng,Y.D., Huang,Q.H., Ren,S.X.,
            Gu,Y.J., Huang,C.H., Li,Y.B., Jiang,C.L., Fu,G., Zhang,Q.H.,
            Gu,B.W., Dai,M., Mao,Y.F., Gao,G.F., Rong,R., Ye,M., Zhou,J.,
            Xu,S.H., Gu,J., Shi,J.X., Jin,W.R., Zhang,C.K., Wu,T.M.,
            Huang,G.Y., Chen,Z., Chen,M.D. and Chen,J.L.
  TITLE     Gene expression profiling in the human
            hypothalamus-pituitary-adrenal axis and full-length cDNA cloning
  JOURNAL   Proc Natl Acad Sci U S A 97 (17), 9543-9548 (2000)
   PUBMED   10931946
REFERENCE   8  (residues 1 to 437)
  AUTHORS   Lippincott-Schwartz,J., Roberts,T.H. and Hirschberg,K.
  TITLE     Secretory protein trafficking and organelle dynamics in living
            cells
  JOURNAL   Annu Rev Cell Dev Biol 16, 557-589 (2000)
   PUBMED   11031247
  REMARK    Review article
REFERENCE   9  (residues 1 to 437)
  AUTHORS   Scheuring,S., Bodor,O., Rohricht,R.A., Muller,S., Beyer,A. and
            Kohrer,K.
  TITLE     Cloning, characterisation, and functional expression of the Mus
            musculus SKD1 gene in yeast demonstrates that the mouse SKD1 and
            the yeast VPS4 genes are orthologues and involved in intracellular
            protein trafficking
  JOURNAL   Gene 234 (1), 149-159 (1999)
   PUBMED   10393249
REFERENCE   10 (residues 1 to 437)
  AUTHORS   Finken-Eigen,M., Rohricht,R.A. and Kohrer,K.
  TITLE     The VPS4 gene is involved in protein transport out of a yeast
            pre-vacuolar endosome-like compartment
  JOURNAL   Curr Genet 31 (6), 469-480 (1997)
   PUBMED   9211789
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC026464.7 and AF255952.1.
            
            Summary: The protein encoded by this gene is a member of the AAA
            protein family (ATPases associated with diverse cellular
            activities), and is the homolog of the yeast Vps4 protein. In
            humans, two paralogs of the yeast protein have been identified. The
            former share a high degree of aa sequence similarity with each
            other, and also with yeast Vps4 and mouse Skd1 proteins. The mouse
            Skd1 (suppressor of K+ transport defect 1) has been shown to be
            really an yeast Vps4 ortholog. Functional studies indicate that
            both human paralogs associate with the endosomal compartments, and
            are involved in intracellular protein trafficking, similar to Vps4
            protein in yeast. The gene encoding this paralog has been mapped to
            chromosome 16; the gene for the other resides on chromosome 18.
            [provided by RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803614.66661.1,
                                           SRR1803617.226430.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000254950.13/ ENSP00000254950.11
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..437
                     /organism="Homo sapiens"
                     /db_xref="taxon:9606"
                     /chromosome="16"
                     /map="16q22.1"
     Protein         1..437
                     /product="vacuolar protein sorting-associated protein 4A"
                     /EC_number="3.6.4.6"
                     /note="vacuolar protein sorting factor 4A; SKD1-homolog;
                     vacuolar sorting protein 4; hVPS4"
                     /calculated_mol_wt=48767
     Region          1..84
                     /region_name="Interaction with CHMP1B"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9UN37.1)"
     Region          4..348
                     /region_name="PTZ00454"
                     /note="26S protease regulatory subunit 6B-like protein;
                     Provisional"
                     /db_xref="CDD:240423"
     Site            8
                     /site_type="acetylation"
                     /note="N6-acetyllysine.
                     /evidence=ECO:0007744|PubMed:19608861; propagated from
                     UniProtKB/Swiss-Prot (Q9UN37.1)"
     Region          75..106
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9UN37.1)"
     Site            95
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q793F9; propagated from
                     UniProtKB/Swiss-Prot (Q9UN37.1)"
     Site            97
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q793F9; propagated from
                     UniProtKB/Swiss-Prot (Q9UN37.1)"
     Region          121..291
                     /region_name="RecA-like_VPS4"
                     /note="ATPase domain of vacuolar protein
                     sorting-associated protein 4; cd19521"
                     /db_xref="CDD:410929"
     Site            order(122,141,145,148..149,153,157,169..170,174,178,189,
                     191,193..195,198,206,227..228,231,236,238,240..242,
                     244..246,249..250,273,276)
                     /site_type="other"
                     /note="hexamer interface [polypeptide binding]"
                     /db_xref="CDD:410929"
     Site            order(128..130,132,169..175,227,272)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:410929"
     Region          374..434
                     /region_name="Vps4_C"
                     /note="Vps4 C terminal oligomerization domain; pfam09336"
                     /db_xref="CDD:462762"
     CDS             1..437
                     /gene="VPS4A"
                     /gene_synonym="CIMDAG; SKD1; SKD1A; SKD2; VPS4; VPS4-1"
                     /coded_by="NM_013245.3:163..1476"
                     /db_xref="CCDS:CCDS45517.1"
                     /db_xref="GeneID:27183"
                     /db_xref="HGNC:HGNC:13488"
                     /db_xref="MIM:609982"
ORIGIN      
        1 mttstlqkai dlvtkateed kaknyeealr lyqhaveyfl haikyeahsd kakesirakc
       61 vqyldraekl kdylrskekh gkkpvkenqs egkgsdsdse gdnpekkklq eqlmgavvme
      121 kpnirwndva glegakealk eavilpikfp hlftgkrtpw rgillfgppg tgksylakav
      181 ateannstff svsssdlmsk wlgeseklvk nlfelarqhk psiifidevd slcgsrnene
      241 seaarrikte flvqmqgvgn nndgtlvlga tnipwvldsa irrrfekriy iplpeeaara
      301 qmfrlhlgst phnltdanih elarktegys gadisiivrd slmqpvrkvq sathfkkvcg
      361 psrtnpsmmi ddlltpcspg dpgamemtwm dvpgdkllep vvcmsdmlrs lattrptvna
      421 ddllkvkkfs edfgqes
//
