LOCUS       NM_007259               2328 bp    mRNA    linear   PRI 11-FEB-2025
DEFINITION  Homo sapiens vacuolar protein sorting 45 homolog (VPS45),
            transcript variant 1, mRNA.
ACCESSION   NM_007259 NM_007258
VERSION     NM_007259.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2328)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   2  (bases 1 to 2328)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   3  (bases 1 to 2328)
  AUTHORS   Frey,L., Zietara,N., Lyszkiewicz,M., Marquardt,B., Mizoguchi,Y.,
            Linder,M.I., Liu,Y., Giesert,F., Wurst,W., Dahlhoff,M.,
            Schneider,M.R., Wolf,E., Somech,R. and Klein,C.
  TITLE     Mammalian VPS45 orchestrates trafficking through the endosomal
            system
  JOURNAL   Blood 137 (14), 1932-1944 (2021)
   PUBMED   33512427
  REMARK    GeneRIF: Mammalian VPS45 orchestrates trafficking through the
            endosomal system.
REFERENCE   4  (bases 1 to 2328)
  AUTHORS   Gao,N. and Ye,B.
  TITLE     SPI1-induced upregulation of lncRNA SNHG6 promotes non-small cell
            lung cancer via miR-485-3p/VPS45 axis
  JOURNAL   Biomed Pharmacother 129, 110239 (2020)
   PUBMED   32590190
  REMARK    GeneRIF: SPI1-induced upregulation of lncRNA SNHG6 promotes
            non-small cell lung cancer via miR-485-3p/VPS45 axis.
REFERENCE   5  (bases 1 to 2328)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   6  (bases 1 to 2328)
  AUTHORS   de Renzis,S., Sonnichsen,B. and Zerial,M.
  TITLE     Divalent Rab effectors regulate the sub-compartmental organization
            and sorting of early endosomes
  JOURNAL   Nat Cell Biol 4 (2), 124-133 (2002)
   PUBMED   11788822
  REMARK    Erratum:[Nat Cell Biol. 2022 Dec;24(12):1776. doi:
            10.1038/s41556-022-01041-4.. PMID: 36471128]
REFERENCE   7  (bases 1 to 2328)
  AUTHORS   Nielsen,E., Christoforidis,S., Uttenweiler-Joseph,S.,
            Miaczynska,M., Dewitte,F., Wilm,M., Hoflack,B. and Zerial,M.
  TITLE     Rabenosyn-5, a novel Rab5 effector, is complexed with hVPS45 and
            recruited to endosomes through a FYVE finger domain
  JOURNAL   J Cell Biol 151 (3), 601-612 (2000)
   PUBMED   11062261
REFERENCE   8  (bases 1 to 2328)
  AUTHORS   Rajasekariah,P., Eyre,H.J., Stanley,K.K., Walls,R.S. and
            Sutherland,G.R.
  TITLE     Molecular cloning and characterization of a cDNA encoding the human
            leucocyte vacuolar protein sorting (h1Vps45)
  JOURNAL   Int J Biochem Cell Biol 31 (6), 683-694 (1999)
   PUBMED   10404641
REFERENCE   9  (bases 1 to 2328)
  AUTHORS   Tellam,J.T., James,D.E., Stevens,T.H. and Piper,R.C.
  TITLE     Identification of a mammalian Golgi Sec1p-like protein, mVps45
  JOURNAL   J Biol Chem 272 (10), 6187-6193 (1997)
   PUBMED   9045632
REFERENCE   10 (bases 1 to 2328)
  AUTHORS   Pevsner,J., Hsu,S.C., Hyde,P.S. and Scheller,R.H.
  TITLE     Mammalian homologues of yeast vacuolar protein sorting (vps) genes
            implicated in Golgi-to-lysosome trafficking
  JOURNAL   Gene 183 (1-2), 7-14 (1996)
   PUBMED   8996080
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BC028382.2 and AW243680.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On May 10, 2018 this sequence version replaced NM_007259.4.
            
            Summary: Vesicle mediated protein sorting plays an important role
            in segregation of intracellular molecules into distinct organelles.
            Genetic studies in yeast have identified more than 40 vacuolar
            protein sorting (VPS) genes involved in vesicle transport to
            vacuoles. This gene is a member of the Sec1 domain family, and
            shows a high degree of sequence similarity to mouse, rat and yeast
            Vps45. The exact function of this gene is not known, but its high
            expression in peripheral blood mononuclear cells suggests a role in
            trafficking proteins, including inflammatory mediators. Multiple
            alternatively spliced transcript variants have been found for this
            gene. [provided by RefSeq, Jul 2013].
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR3476690.998441.1, BC028382.2
                                           [ECO:0000332]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000644510.2/ ENSP00000495563.1
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2326              BC028382.2         465-2790
            2327-2328           AW243680.1         14-15               c
FEATURES             Location/Qualifiers
     source          1..2328
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1q21.2"
     gene            1..2328
                     /gene="VPS45"
                     /gene_synonym="H1; H1VPS45; SCN5; VPS45A; VPS45B; VPS54A;
                     VSP45; VSP45A"
                     /note="vacuolar protein sorting 45 homolog"
                     /db_xref="GeneID:11311"
                     /db_xref="HGNC:HGNC:14579"
                     /db_xref="MIM:610035"
     exon            1..175
                     /gene="VPS45"
                     /gene_synonym="H1; H1VPS45; SCN5; VPS45A; VPS45B; VPS54A;
                     VSP45; VSP45A"
                     /inference="alignment:Splign:2.1.0"
     CDS             83..1795
                     /gene="VPS45"
                     /gene_synonym="H1; H1VPS45; SCN5; VPS45A; VPS45B; VPS54A;
                     VSP45; VSP45A"
                     /note="isoform 1 is encoded by transcript variant 1;
                     vacuolar protein sorting-associated protein 45; leucocyte
                     vacuolar protein sorting 45; vacuolar protein sorting 45A"
                     /codon_start=1
                     /product="vacuolar protein sorting-associated protein 45
                     isoform 1"
                     /protein_id="NP_009190.2"
                     /db_xref="CCDS:CCDS944.1"
                     /db_xref="GeneID:11311"
                     /db_xref="HGNC:HGNC:14579"
                     /db_xref="MIM:610035"
                     /translation="MNVVFAVKQYISKMIEDSGPGMKVLLMDKETTGIVSMVYTQSEI
                     LQKEVYLFERIDSQNREIMKHLKAICFLRPTKENVDYIIQELRRPKYTIYFIYFSNVI
                     SKSDVKSLAEADEQEVVAEVQEFYGDYIAVNPHLFSLNILGCCQGRNWDPAQLSRTTQ
                     GLTALLLSLKKCPMIRYQLSSEAAKRLAECVKQVITKEYELFEFRRTEVPPLLLILDR
                     CDDAITPLLNQWTYQAMVHELLGINNNRIDLSRVPGISKDLREVVLSAENDEFYANNM
                     YLNFAEIGSNIKNLMEDFQKKKPKEQQKLESIADMKAFVENYPQFKKMSGTVSKHVTV
                     VGELSRLVSERNLLEVSEVEQELACQNDHSSALQNIKRLLQNPKVTEFDAARLVMLYA
                     LHYERHSSNSLPGLMMDLRNKGVSEKYRKLVSAVVEYGGKRVRGSDLFSPKDAVAITK
                     QFLKGLKGVENVYTQHQPFLHETLDHLIKGRLKENLYPYLGPSTLRDRPQDIIVFVIG
                     GATYEEALTVYNLNRTTPGVRIVLGGTTVHNTKSFLEEVLASGLHSRSKESSQVTSRS
                     ASRR"
     exon            176..310
                     /gene="VPS45"
                     /gene_synonym="H1; H1VPS45; SCN5; VPS45A; VPS45B; VPS54A;
                     VSP45; VSP45A"
                     /inference="alignment:Splign:2.1.0"
     exon            311..371
                     /gene="VPS45"
                     /gene_synonym="H1; H1VPS45; SCN5; VPS45A; VPS45B; VPS54A;
                     VSP45; VSP45A"
                     /inference="alignment:Splign:2.1.0"
     exon            372..451
                     /gene="VPS45"
                     /gene_synonym="H1; H1VPS45; SCN5; VPS45A; VPS45B; VPS54A;
                     VSP45; VSP45A"
                     /inference="alignment:Splign:2.1.0"
     exon            452..520
                     /gene="VPS45"
                     /gene_synonym="H1; H1VPS45; SCN5; VPS45A; VPS45B; VPS54A;
                     VSP45; VSP45A"
                     /inference="alignment:Splign:2.1.0"
     exon            521..658
                     /gene="VPS45"
                     /gene_synonym="H1; H1VPS45; SCN5; VPS45A; VPS45B; VPS54A;
                     VSP45; VSP45A"
                     /inference="alignment:Splign:2.1.0"
     exon            659..769
                     /gene="VPS45"
                     /gene_synonym="H1; H1VPS45; SCN5; VPS45A; VPS45B; VPS54A;
                     VSP45; VSP45A"
                     /inference="alignment:Splign:2.1.0"
     exon            770..904
                     /gene="VPS45"
                     /gene_synonym="H1; H1VPS45; SCN5; VPS45A; VPS45B; VPS54A;
                     VSP45; VSP45A"
                     /inference="alignment:Splign:2.1.0"
     exon            905..1018
                     /gene="VPS45"
                     /gene_synonym="H1; H1VPS45; SCN5; VPS45A; VPS45B; VPS54A;
                     VSP45; VSP45A"
                     /inference="alignment:Splign:2.1.0"
     exon            1019..1186
                     /gene="VPS45"
                     /gene_synonym="H1; H1VPS45; SCN5; VPS45A; VPS45B; VPS54A;
                     VSP45; VSP45A"
                     /inference="alignment:Splign:2.1.0"
     exon            1187..1345
                     /gene="VPS45"
                     /gene_synonym="H1; H1VPS45; SCN5; VPS45A; VPS45B; VPS54A;
                     VSP45; VSP45A"
                     /inference="alignment:Splign:2.1.0"
     exon            1346..1453
                     /gene="VPS45"
                     /gene_synonym="H1; H1VPS45; SCN5; VPS45A; VPS45B; VPS54A;
                     VSP45; VSP45A"
                     /inference="alignment:Splign:2.1.0"
     exon            1454..1575
                     /gene="VPS45"
                     /gene_synonym="H1; H1VPS45; SCN5; VPS45A; VPS45B; VPS54A;
                     VSP45; VSP45A"
                     /inference="alignment:Splign:2.1.0"
     exon            1576..1707
                     /gene="VPS45"
                     /gene_synonym="H1; H1VPS45; SCN5; VPS45A; VPS45B; VPS54A;
                     VSP45; VSP45A"
                     /inference="alignment:Splign:2.1.0"
     exon            1708..2328
                     /gene="VPS45"
                     /gene_synonym="H1; H1VPS45; SCN5; VPS45A; VPS45B; VPS54A;
                     VSP45; VSP45A"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2302..2307
                     /regulatory_class="polyA_signal_sequence"
                     /gene="VPS45"
                     /gene_synonym="H1; H1VPS45; SCN5; VPS45A; VPS45B; VPS54A;
                     VSP45; VSP45A"
                     /note="hexamer: AATAAA"
     polyA_site      2326
                     /gene="VPS45"
                     /gene_synonym="H1; H1VPS45; SCN5; VPS45A; VPS45B; VPS54A;
                     VSP45; VSP45A"
                     /note="major polyA site"
ORIGIN      
        1 gagacccggc caacagactg ggggttaatt tagccagaaa agggggcggg aagggctgta
       61 gggtacttgt caattcgccg ccatgaacgt ggtttttgct gtgaagcagt acatttccaa
      121 aatgatagag gacagcgggc ctggtatgaa agtacttctc atggataaag agacgactgg
      181 catagtgagt atggtataca cacaatcgga gattctacag aaggaagtgt acctctttga
      241 acgcattgat tctcaaaatc gagagatcat gaaacacctg aaggcaattt gttttcttcg
      301 acctacaaag gagaatgtgg attatattat tcaggagctc cgaagaccca aatacactat
      361 atatttcatt tatttcagta atgtgatcag caagagtgac gtgaagtcat tggctgaagc
      421 tgatgaacag gaagttgtgg ctgaggttca ggaattttat ggtgattaca ttgctgtgaa
      481 cccacatttg ttttccctca atattttggg ttgctgccag ggtcgaaatt gggatccagc
      541 ccagctatct agaacaactc aagggcttac agctctcctt ttatctctga agaagtgtcc
      601 catgattcgt tatcagctct catcagaggc agcaaagaga cttgcagagt gcgttaagca
      661 agtgataact aaagaatatg aactgtttga attccgtcgg acagaggttc ctccattgct
      721 ccttatttta gatcgctgtg atgatgccat caccccattg ctaaaccagt ggacatatca
      781 ggccatggtc cacgaactac taggcataaa caacaatcgg attgatcttt ccagagtgcc
      841 gggaatcagt aaagacttaa gagaagtggt cctatctgct gaaaatgatg aattctatgc
      901 taataatatg tacctgaact ttgctgagat tggtagcaat ataaagaatc tcatggaaga
      961 ttttcagaag aagaaaccaa aagaacagca aaaactagaa tcaatagcag acatgaaggc
     1021 gtttgttgag aattatccac agttcaagaa aatgtctggg actgtttcaa agcatgtgac
     1081 agtggttgga gaactgtctc gattggtcag tgaacggaat ctgctggagg tttcagaggt
     1141 tgagcaagaa ctggcctgtc aaaatgacca ttctagtgct ctccagaata taaaaaggct
     1201 tctgcagaac cccaaagtga cagagtttga tgctgcccgc ctggtgatgc tttatgcttt
     1261 acattatgag cgacacagca gcaatagcct gccaggacta atgatggacc tcaggaataa
     1321 aggtgtttct gagaagtatc gaaagctcgt gtctgcagtt gttgaatatg gtggtaaacg
     1381 agtcagagga agtgacctct tcagccccaa agatgctgtg gctatcacca aacaattcct
     1441 caaaggactg aagggagtag aaaatgtata tacacagcat caacctttcc tacatgaaac
     1501 cctggatcat ctcatcaaag gaaggcttaa ggaaaaccta tatccttatt taggccccag
     1561 cacactcaga gacagacctc aggatatcat tgtgtttgta attggaggag ccacctatga
     1621 agaggctcta acagtttata acctgaaccg caccactcct ggagtgagga ttgtcctggg
     1681 aggcaccaca gtgcacaaca cgaaaagttt cctagaggaa gttctggctt ctggactgca
     1741 cagccgaagc aaggagagct ctcaagtcac atcaaggtca gcgagcagaa gatgaaacgg
     1801 tggttggggg aagggcacag cttcctctct tgtccccact acaggttttc cctactaaac
     1861 aaaggtgttg gagagcagct ttgggttctg tgctggttgt tagaactcat ctccaggtag
     1921 cccacggata cgtggttggc acagacacaa gactcccaga gttgtcctaa caataagtct
     1981 gagcccatct caacccactt ttctccggta gtctttatgt atctgttagc acaatcactt
     2041 cagttactga tgaattttgt tgggatctga cttggggaaa gggttatcag agcctagagg
     2101 ggcttaaaaa gtaatcattt gatgtacata ccacactcct tggcttcctt tctcttccct
     2161 taaccctttc tgcttttcat taaccacatt cctgcacaac tcatttctga aaacctacca
     2221 tgtttcttta cagagccatc caaaaatttt ttgtccctac atagcaattt tctgtggcac
     2281 tgagaaacca tgtatgacca caataaaaat ccattttgtg aaaggata
//
