LOCUS       NM_014396               5854 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens VPS41 subunit of HOPS complex (VPS41), transcript
            variant 1, mRNA.
ACCESSION   NM_014396
VERSION     NM_014396.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5854)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   2  (bases 1 to 5854)
  AUTHORS   Chen,Z., Wang,C., Feng,X., Nie,L., Tang,M., Zhang,H., Xiong,Y.,
            Swisher,S.K., Srivastava,M. and Chen,J.
  TITLE     Interactomes of SARS-CoV-2 and human coronaviruses reveal host
            factors potentially affecting pathogenesis
  JOURNAL   EMBO J 40 (17), e107776 (2021)
   PUBMED   34232536
REFERENCE   3  (bases 1 to 5854)
  AUTHORS   Stukalov,A., Girault,V., Grass,V., Karayel,O., Bergant,V.,
            Urban,C., Haas,D.A., Huang,Y., Oubraham,L., Wang,A., Hamad,M.S.,
            Piras,A., Hansen,F.M., Tanzer,M.C., Paron,I., Zinzula,L.,
            Engleitner,T., Reinecke,M., Lavacca,T.M., Ehmann,R., Wolfel,R.,
            Jores,J., Kuster,B., Protzer,U., Rad,R., Ziebuhr,J., Thiel,V.,
            Scaturro,P., Mann,M. and Pichlmair,A.
  TITLE     Multilevel proteomics reveals host perturbations by SARS-CoV-2 and
            SARS-CoV
  JOURNAL   Nature 594 (7862), 246-252 (2021)
   PUBMED   33845483
REFERENCE   4  (bases 1 to 5854)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   5  (bases 1 to 5854)
  AUTHORS   van der Welle,R.E.N., Jobling,R., Burns,C., Sanza,P., van der
            Beek,J.A., Fasano,A., Chen,L., Zwartkruis,F.J., Zwakenberg,S.,
            Griffin,E.F., Ten Brink,C., Veenendaal,T., Liv,N., van
            Ravenswaaij-Arts,C.M.A., Lemmink,H.H., Pfundt,R., Blaser,S.,
            Sepulveda,C., Lozano,A.M., Yoon,G., Santiago-Sim,T., Asensio,C.S.,
            Caldwell,G.A., Caldwell,K.A., Chitayat,D. and Klumperman,J.
  TITLE     Neurodegenerative VPS41 variants inhibit HOPS function and
            mTORC1-dependent TFEB/TFE3 regulation
  JOURNAL   EMBO Mol Med 13 (5), e13258 (2021)
   PUBMED   33851776
  REMARK    GeneRIF: Neurodegenerative VPS41 variants inhibit HOPS function and
            mTORC1-dependent TFEB/TFE3 regulation.
REFERENCE   6  (bases 1 to 5854)
  AUTHORS   Schroder,B., Wrocklage,C., Pan,C., Jager,R., Kosters,B.,
            Schafer,H., Elsasser,H.P., Mann,M. and Hasilik,A.
  TITLE     Integral and associated lysosomal membrane proteins
  JOURNAL   Traffic 8 (12), 1676-1686 (2007)
   PUBMED   17897319
REFERENCE   7  (bases 1 to 5854)
  AUTHORS   McVey Ward,D., Radisky,D., Scullion,M.A., Tuttle,M.S., Vaughn,M.
            and Kaplan,J.
  TITLE     hVPS41 is expressed in multiple isoforms and can associate with
            vesicles through a RING-H2 finger motif
  JOURNAL   Exp Cell Res 267 (1), 126-134 (2001)
   PUBMED   11412045
REFERENCE   8  (bases 1 to 5854)
  AUTHORS   Rehling,P., Darsow,T., Katzmann,D.J. and Emr,S.D.
  TITLE     Formation of AP-3 transport intermediates requires Vps41 function
  JOURNAL   Nat Cell Biol 1 (6), 346-353 (1999)
   PUBMED   10559961
REFERENCE   9  (bases 1 to 5854)
  AUTHORS   Radisky,D.C., Snyder,W.B., Emr,S.D. and Kaplan,J.
  TITLE     Characterization of VPS41, a gene required for vacuolar trafficking
            and high-affinity iron transport in yeast
  JOURNAL   Proc Natl Acad Sci U S A 94 (11), 5662-5666 (1997)
   PUBMED   9159129
REFERENCE   10 (bases 1 to 5854)
  AUTHORS   Sherrington,R., Rogaev,E.I., Liang,Y., Rogaeva,E.A., Levesque,G.,
            Ikeda,M., Chi,H., Lin,C., Li,G., Holman,K., Tsuda,T., Mar,L.,
            Foncin,J.F., Bruni,A.C., Montesi,M.P., Sorbi,S., Rainero,I.,
            Pinessi,L., Nee,L., Chumakov,I., Pollen,D., Brookes,A., Sanseau,P.,
            Polinsky,R.J., Wasco,W., Da Silva,H.A., Haines,J.L.,
            Perkicak-Vance,M.A., Tanzi,R.E., Roses,A.D., Fraser,P.E.,
            Rommens,J.M. and St George-Hyslop,P.H.
  TITLE     Cloning of a gene bearing missense mutations in early-onset
            familial Alzheimer's disease
  JOURNAL   Nature 375 (6534), 754-760 (1995)
   PUBMED   7596406
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from U87309.1, BC044851.1 and
            AC004850.3.
            
            On Nov 22, 2018 this sequence version replaced NM_014396.3.
            
            Summary: Vesicle mediated protein sorting plays an important role
            in segregation of intracellular molecules into distinct organelles.
            Genetic studies in yeast have identified more than 40 vacuolar
            protein sorting (VPS) genes involved in vesicle transport to
            vacuoles. This gene encodes the human ortholog of yeast Vps41
            protein which is also conserved in Drosophila, tomato, and
            Arabidopsis. Expression studies in yeast and human indicate that
            this protein may be involved in the formation and fusion of
            transport vesicles from the Golgi. Several transcript variants
            encoding different isoforms have been described for this gene,
            however, the full-length nature of not all is known. [provided by
            RefSeq, Jul 2008].
            
            Transcript Variant: This variant (1) encodes the full-length
            isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803614.23467.1,
                                           SRR1660803.90518.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000310301.9/ ENSP00000309457.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-30                U87309.1           13-42
            31-3538             BC044851.1         35-3542
            3539-4885           AC004850.3         30982-32328         c
            4886-5854           AC004850.3         30013-30981         c
FEATURES             Location/Qualifiers
     source          1..5854
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="7"
                     /map="7p14.1"
     gene            1..5854
                     /gene="VPS41"
                     /gene_synonym="HVPS41; hVps41p; HVSP41; SCAR29"
                     /note="VPS41 subunit of HOPS complex"
                     /db_xref="GeneID:27072"
                     /db_xref="HGNC:HGNC:12713"
                     /db_xref="MIM:605485"
     exon            1..38
                     /gene="VPS41"
                     /gene_synonym="HVPS41; hVps41p; HVSP41; SCAR29"
                     /inference="alignment:Splign:2.1.0"
     CDS             18..2582
                     /gene="VPS41"
                     /gene_synonym="HVPS41; hVps41p; HVSP41; SCAR29"
                     /note="isoform 1 is encoded by transcript variant 1;
                     vacuolar assembly protein 41; vacuolar protein
                     sorting-associated protein 41 homolog; S53; VPS41, HOPS
                     complex subunit; vacuolar protein sorting 41 homolog"
                     /codon_start=1
                     /product="vacuolar protein sorting-associated protein 41
                     homolog isoform 1"
                     /protein_id="NP_055211.2"
                     /db_xref="CCDS:CCDS5457.1"
                     /db_xref="GeneID:27072"
                     /db_xref="HGNC:HGNC:12713"
                     /db_xref="MIM:605485"
                     /translation="MAEAEEQETGSLEESTDESEEEESEEEPKLKYERLSNGVTEILQ
                     KDAASCMTVHDKFLALGTHYGKVYLLDVQGNITQKFDVSPVKINQISLDESGEHMGVC
                     SEDGKVQVFGLYSGEEFHETFDCPIKIIAVHPHFVRSSCKQFVTGGKKLLLFERSWMN
                     RWKSAVLHEGEGNIRSVKWRGHLIAWANNMGVKIFDIISKQRITNVPRDDISLRPDMY
                     PCSLCWKDNVTLIIGWGTSVKVCSVKERHASEMRDLPSRYVEIVSQFETEFYISGLAP
                     LCDQLVVLSYVKEISEKTEREYCARPRLDIIQPLSETCEEISSDALTVRGFQENECRD
                     YHLEYSEGESLFYIVSPRDVVVAKERDQDDHIDWLLEKKKYEEALMAAEISQKNIKRH
                     KILDIGLAYINHLVERGDYDIAARKCQKILGKNAALWEYEVYKFKEIGQLKAISPYLP
                     RGDPVLKPLIYEMILHEFLESDYEGFATLIREWPGDLYNNSVIVQAVRDHLKKDSQNK
                     TLLKTLAELYTYDKNYGNALEIYLTLRHKDVFQLIHKHNLFSSIKDKIVLLMDFDSEK
                     AVDMLLDNEDKISIKKVVEELEDRPELQHVYLHKLFKRDHHKGQRYHEKQISLYAEYD
                     RPNLLPFLRDSTHCPLEKALEICQQRNFVEETVYLLSRMGNSRSALKMIMEELHDVDK
                     AIEFAKEQDDGELWEDLILYSIDKPPFITGLLNNIGTHVDPILLIHRIKEGMEIPNLR
                     DSLVKILQDYNLQILLREGCKKILVADSLSLLKKMHRTQMKGVLVDEENICESCLSPI
                     LPSDAAKPFSVVVFHCRHMFHKECLPMPSMNSAAQFCNICSAKNRGPGSAILEMKK"
     exon            39..77
                     /gene="VPS41"
                     /gene_synonym="HVPS41; hVps41p; HVSP41; SCAR29"
                     /inference="alignment:Splign:2.1.0"
     exon            78..185
                     /gene="VPS41"
                     /gene_synonym="HVPS41; hVps41p; HVSP41; SCAR29"
                     /inference="alignment:Splign:2.1.0"
     exon            186..263
                     /gene="VPS41"
                     /gene_synonym="HVPS41; hVps41p; HVSP41; SCAR29"
                     /inference="alignment:Splign:2.1.0"
     exon            264..338
                     /gene="VPS41"
                     /gene_synonym="HVPS41; hVps41p; HVSP41; SCAR29"
                     /inference="alignment:Splign:2.1.0"
     exon            339..401
                     /gene="VPS41"
                     /gene_synonym="HVPS41; hVps41p; HVSP41; SCAR29"
                     /inference="alignment:Splign:2.1.0"
     exon            402..467
                     /gene="VPS41"
                     /gene_synonym="HVPS41; hVps41p; HVSP41; SCAR29"
                     /inference="alignment:Splign:2.1.0"
     exon            468..587
                     /gene="VPS41"
                     /gene_synonym="HVPS41; hVps41p; HVSP41; SCAR29"
                     /inference="alignment:Splign:2.1.0"
     exon            588..734
                     /gene="VPS41"
                     /gene_synonym="HVPS41; hVps41p; HVSP41; SCAR29"
                     /inference="alignment:Splign:2.1.0"
     exon            735..801
                     /gene="VPS41"
                     /gene_synonym="HVPS41; hVps41p; HVSP41; SCAR29"
                     /inference="alignment:Splign:2.1.0"
     exon            802..899
                     /gene="VPS41"
                     /gene_synonym="HVPS41; hVps41p; HVSP41; SCAR29"
                     /inference="alignment:Splign:2.1.0"
     exon            900..1029
                     /gene="VPS41"
                     /gene_synonym="HVPS41; hVps41p; HVSP41; SCAR29"
                     /inference="alignment:Splign:2.1.0"
     exon            1030..1145
                     /gene="VPS41"
                     /gene_synonym="HVPS41; hVps41p; HVSP41; SCAR29"
                     /inference="alignment:Splign:2.1.0"
     exon            1146..1202
                     /gene="VPS41"
                     /gene_synonym="HVPS41; hVps41p; HVSP41; SCAR29"
                     /inference="alignment:Splign:2.1.0"
     exon            1203..1264
                     /gene="VPS41"
                     /gene_synonym="HVPS41; hVps41p; HVSP41; SCAR29"
                     /inference="alignment:Splign:2.1.0"
     exon            1265..1346
                     /gene="VPS41"
                     /gene_synonym="HVPS41; hVps41p; HVSP41; SCAR29"
                     /inference="alignment:Splign:2.1.0"
     exon            1347..1439
                     /gene="VPS41"
                     /gene_synonym="HVPS41; hVps41p; HVSP41; SCAR29"
                     /inference="alignment:Splign:2.1.0"
     exon            1440..1567
                     /gene="VPS41"
                     /gene_synonym="HVPS41; hVps41p; HVSP41; SCAR29"
                     /inference="alignment:Splign:2.1.0"
     exon            1568..1712
                     /gene="VPS41"
                     /gene_synonym="HVPS41; hVps41p; HVSP41; SCAR29"
                     /inference="alignment:Splign:2.1.0"
     exon            1713..1754
                     /gene="VPS41"
                     /gene_synonym="HVPS41; hVps41p; HVSP41; SCAR29"
                     /inference="alignment:Splign:2.1.0"
     exon            1755..1805
                     /gene="VPS41"
                     /gene_synonym="HVPS41; hVps41p; HVSP41; SCAR29"
                     /inference="alignment:Splign:2.1.0"
     exon            1806..1943
                     /gene="VPS41"
                     /gene_synonym="HVPS41; hVps41p; HVSP41; SCAR29"
                     /inference="alignment:Splign:2.1.0"
     exon            1944..1998
                     /gene="VPS41"
                     /gene_synonym="HVPS41; hVps41p; HVSP41; SCAR29"
                     /inference="alignment:Splign:2.1.0"
     exon            1999..2139
                     /gene="VPS41"
                     /gene_synonym="HVPS41; hVps41p; HVSP41; SCAR29"
                     /inference="alignment:Splign:2.1.0"
     exon            2140..2276
                     /gene="VPS41"
                     /gene_synonym="HVPS41; hVps41p; HVSP41; SCAR29"
                     /inference="alignment:Splign:2.1.0"
     exon            2277..2376
                     /gene="VPS41"
                     /gene_synonym="HVPS41; hVps41p; HVSP41; SCAR29"
                     /inference="alignment:Splign:2.1.0"
     exon            2377..2421
                     /gene="VPS41"
                     /gene_synonym="HVPS41; hVps41p; HVSP41; SCAR29"
                     /inference="alignment:Splign:2.1.0"
     exon            2422..2501
                     /gene="VPS41"
                     /gene_synonym="HVPS41; hVps41p; HVSP41; SCAR29"
                     /inference="alignment:Splign:2.1.0"
     exon            2502..5854
                     /gene="VPS41"
                     /gene_synonym="HVPS41; hVps41p; HVSP41; SCAR29"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3251..3256
                     /regulatory_class="polyA_signal_sequence"
                     /gene="VPS41"
                     /gene_synonym="HVPS41; hVps41p; HVSP41; SCAR29"
                     /note="hexamer: AATAAA"
     polyA_site      3274
                     /gene="VPS41"
                     /gene_synonym="HVPS41; hVps41p; HVSP41; SCAR29"
                     /note="major polyA site"
     regulatory      3664..3669
                     /regulatory_class="polyA_signal_sequence"
                     /gene="VPS41"
                     /gene_synonym="HVPS41; hVps41p; HVSP41; SCAR29"
                     /note="hexamer: CATAAA"
     polyA_site      3683
                     /gene="VPS41"
                     /gene_synonym="HVPS41; hVps41p; HVSP41; SCAR29"
     regulatory      4818..4823
                     /regulatory_class="polyA_signal_sequence"
                     /gene="VPS41"
                     /gene_synonym="HVPS41; hVps41p; HVSP41; SCAR29"
                     /note="hexamer: AATAAA"
     polyA_site      4845
                     /gene="VPS41"
                     /gene_synonym="HVPS41; hVps41p; HVSP41; SCAR29"
     regulatory      5837..5842
                     /regulatory_class="polyA_signal_sequence"
                     /gene="VPS41"
                     /gene_synonym="HVPS41; hVps41p; HVSP41; SCAR29"
                     /note="hexamer: AATAAA"
     polyA_site      5854
                     /gene="VPS41"
                     /gene_synonym="HVPS41; hVps41p; HVSP41; SCAR29"
ORIGIN      
        1 gactctcccg tggcgccatg gcggaagcag aggagcagga aactgggtcc cttgaagaat
       61 ctacagatga gtctgaggaa gaagagagcg aagaggaacc caagctgaag tatgaaaggc
      121 tttccaatgg ggtaactgaa atacttcaga aggatgcagc tagctgcatg acagtccatg
      181 acaagttttt ggcattgggc acacattatg gcaaggttta tttacttgat gtccagggga
      241 acatcactca gaagtttgat gtaagtcctg tgaagataaa tcagattagc ttggatgaaa
      301 gtggagagca catgggtgtg tgttcagagg atggcaaggt gcaggtattt ggactgtatt
      361 ctggagaaga atttcacgag acttttgact gtcccattaa aattattgct gtgcacccac
      421 atttcgtgag atccagttgc aagcagtttg tgaccggagg gaagaagctg ctactgtttg
      481 aacggtcttg gatgaacaga tggaagtctg ctgttctgca tgaaggggaa gggaacataa
      541 ggagtgtgaa gtggagaggc catctgattg cttgggccaa taatatgggt gtgaagattt
      601 ttgacatcat ctcaaagcaa agaatcacca atgtgccccg ggatgatata agtcttcgcc
      661 cagacatgta tccctgcagc ctctgctgga aggacaatgt gacactgatt attggctggg
      721 ggacttctgt caaggtgtgc tcagtgaagg aacggcatgc cagtgaaatg agggatttgc
      781 caagtcgata tgttgaaata gtgtctcagt ttgaaactga attctacatc agtggacttg
      841 cacctctctg tgatcagctt gttgtacttt cgtatgtaaa ggagatttca gaaaaaacgg
      901 aaagagaata ctgtgccagg cctagactgg acatcatcca gccactttct gagacttgtg
      961 aagagatctc ttctgatgct ttgacagtca gaggctttca ggagaatgaa tgtagagatt
     1021 atcatttaga atactctgaa ggggaatcac ttttttacat cgtgagtccg agagatgttg
     1081 tagtggccaa ggaacgagac caagatgatc acattgactg gctccttgaa aagaagaaat
     1141 atgaagaagc attgatggca gctgaaatta gccaaaaaaa tattaaaaga cataagattc
     1201 tggatattgg cttggcatat ataaatcacc tggtggagag aggagactat gacatagcag
     1261 cacgcaaatg ccagaaaatt cttgggaaaa atgcagcact ctgggaatat gaagtttata
     1321 aatttaaaga aattggacag cttaaggcta ttagtcctta tttgccaaga ggtgatccag
     1381 ttctgaaacc actcatctat gaaatgatct tacatgaatt tttggagagt gattatgagg
     1441 gttttgccac attgatccga gaatggcctg gagatctgta taataattca gtcatagttc
     1501 aagcagttcg ggatcatttg aagaaagata gtcagaacaa gactttactt aaaaccctgg
     1561 cagaattgta cacctatgac aagaactatg gcaatgctct ggaaatatac ttaacattaa
     1621 gacataaaga cgtttttcag ttgatccaca agcataatct tttcagttct atcaaggata
     1681 aaattgtttt attaatggat tttgattcag agaaagctgt tgacatgctt ttggacaatg
     1741 aagataaaat ttcaattaaa aaggtagtgg aagaattgga agacagacca gagctacagc
     1801 atgtgtattt gcataagctt ttcaagagag accaccataa ggggcagcgt taccatgaaa
     1861 aacagatcag tctttatgct gaatatgatc gaccaaactt acttcccttt ctccgagaca
     1921 gtacccattg cccacttgaa aaggctcttg agatctgtca acagagaaac tttgtagaag
     1981 agacagttta tcttctgagc cgaatgggta atagccgaag tgccctgaag atgattatgg
     2041 aggaattaca tgatgttgat aaagcaatcg aatttgccaa ggagcaagat gatggagagc
     2101 tgtgggaaga tttgatttta tattccattg acaaaccacc atttattact ggcttgttaa
     2161 acaacattgg cacacatgtt gacccaattc tactgattca ccgtattaag gaaggaatgg
     2221 agatccccaa tttgagagat tccttggtta aaattctgca agactacaat ttgcaaattc
     2281 tgcttcgtga aggctgcaag aagattctcg tagctgactc tttgtcctta ctgaagaaaa
     2341 tgcaccgaac tcaaatgaaa ggtgttcttg ttgatgagga gaacatctgt gagtcgtgcc
     2401 tttcccctat tcttccatca gatgcagcta agcccttcag cgtggtggtc ttccattgcc
     2461 ggcacatgtt ccacaaggag tgcctgccca tgcccagcat gaactctgct gcacagttct
     2521 gcaacatctg cagtgctaag aaccgtggac caggaagtgc aattttggag atgaaaaaat
     2581 agctcatttc tccttgtcag tctccttgtc accactcttt ttgagactgt ttttgcaaca
     2641 acaaaagcat ttgttgacac tcgtgctgtt aagagatttg tttatgttta tattatactc
     2701 aaaaacaatt tcttcatcta ttcctgtact aatggtttct ctttgcagtt cacagagaat
     2761 ttggggctct cttcatgcct tgaaattttg gggtccatag tgaatatttt gttatttatt
     2821 tgtttggctc attctttata tagtaatgga aacataagtc taggagttag aaatgaattt
     2881 tttagacctt agtaaaacca tttaaccata aaatggacaa ctgagaattc tcccagctgc
     2941 ctgaaagcgt cgccaactgt ggttatcctg caagctgcta cctgcaactt ggacgttgtt
     3001 tccacgtgct ctgctggcta cgattcttgc attctgggtt tggctttttt ctgtgtcatc
     3061 aactatggtt atcctctaaa taggcattta atgaaacatt gtacaaattg tcactcattt
     3121 gatgacacct gggaataaca ttagcaggct gatgtcctgc accattatgt ttactaatca
     3181 catgttctgt gtgctgtgac gactgtcaaa gagtatctgg ccatggcgga cactcagcat
     3241 ttgttgattg aataaatgtt agctcttctc attgtgaagg actcactttt actgggataa
     3301 acaaatgcag ttaagaattc tggcaccctt gtaaggaaga aaagagagtt caacaccttc
     3361 gagtctgagc gcttgtggct agagtttgcc aggagggagg aaaccagtga ccctgaaaac
     3421 tgagggtgcc tcaggagcag tgggaccacc tgatgctgaa ggacggacta atgatgtttc
     3481 ctcttgcctt ctctggtgcc tccattgccc tcatggaaca gagcatatca tagagggaga
     3541 aaagtcaaac ttgtaattgt gtcttacagt tactggcttc atcttccttg ggatatatgg
     3601 tcatcctcta atgagtgtaa aagtgcgcaa aacacatcct tattgttcct gatctcttag
     3661 tcccataaat gggaacaaat acagctttct gcttctttct ttttggggaa aggacagggt
     3721 gctagtgagt actgacagca tgccagctac cgaagtcacc cagccattcc catgagcagc
     3781 agttcattta attgtcacag cgtcgccagg aagaagatct gataaaccta ggtttacaga
     3841 taaagaaagc aaaatgtaga gatgttgttg aggtcacaga ggtgactgcc taacttcaga
     3901 gcagggcttc tgatcccttt aagaaattac agggccagcc gggcatggtg gctcacgccc
     3961 gtaatcccag ggctttggga ggccttggca ggtggatcac ctgagatcgc acgttcgaga
     4021 ccagcctgac caacatggag aaaccccatc tctactaaaa acacaaatta gccaggcgtg
     4081 gtggtacatg cctgtaatcc cagctactca ggaggctgag gcaggagaat cacttgaccc
     4141 caggagacgt aggttgtggt gagctgagat cgcgccattg cactccagcc tgggcaacaa
     4201 gagcaaaact ccgtctcaaa aaagaaaaga aaagaaaaga aatcataggg ccaagttcaa
     4261 aggaaatgca cagaacatat cttcacatta gagttaagaa ttctctagca aacaacagat
     4321 ttttttgttg ttgttagtca caaatactta gaactggaag gctctttgtt attattgaat
     4381 gtacccctca gccttctcag catttcctta tcccaagact agtgtgcttt ctgctacact
     4441 gctagttttc agttttgttc ttacccaatt gttttttctt ttcaacatta ccaatttaca
     4501 gattcagttt attacattta cattaatcct cacttatgat ttgagcaagc tcatttccag
     4561 aaaagtttac tttaagatca tcaataggat ttgctaattt cagtgaagtc attttgcttc
     4621 aggggtaaat tatcctagtt accaagtcct atttggacat aaagaaaatc ctacttatag
     4681 aaaaggagaa aataattaaa cagtcttcat ttttaagtaa ctgatttaaa aggaaaataa
     4741 taaaatatgt tcgtttatca tttcagaaat tgctgtaaca cactggaaaa ttcctgaaca
     4801 atatagattt tatcgttaat aaaaaacact agctttcgtt ccttagaatg tcttttcttt
     4861 tgaataaaca gtattgggtg atttattaaa ggatattcaa tcctttttac ttttcatcaa
     4921 cctataaggg cttaaatatg gcaaaataat ccaagtgtta gtcaattatt tttcatgtgt
     4981 gactagccca atttaaaata taggtaaaaa ggaaacccat tcccctcgtt ttggggaaga
     5041 acatagttgc aaagtacaca cttcataagc tattcttttt tttttttttt tttttttttt
     5101 ttttgagatg gagtctcgtt ctgttgcctt ggctggagtg cagtgacatg atcttggctc
     5161 actgcaacct ccgcctccca ggttcaagtg attctcccgt ctcagcttcc ggagtaggtg
     5221 ggagtacagg cacgcaccac catgcccagc taattttttg tactttggta gagatgaggt
     5281 ttcaccatgt tgcccaagct ggtctcgaac tcctgagctc aggaatccgc ccacctcggc
     5341 cttccgaagt gttaggatta caggcgtaag tcaccatgcc cagccaagaa agctattctt
     5401 gaagtgtaaa acagccaggt gcaaaattat tttggagtac tttagtttgc ttttgttttt
     5461 agccaaaggg atttcatact ggtttaatta tgttcatcct tgagaatttt tgagaagttt
     5521 ccaacagatt tgtagttcat tttgctgttg ttacaatgta aagctatata tagttgaccc
     5581 ttgaacaaca caggttggaa ctgcaaggat ccacttatac atggattttc ttctgcctct
     5641 gccacccttg agacggcaag accaggccct ccccttcctc ctccttctca gcctactcag
     5701 tatgaagacg acagagatga aacacttatg atgatccact tctacttaat gagtagtaaa
     5761 tatattttct tttccttatg attttcttac tattttcttt agcttgcttt attataagaa
     5821 tatagcatat aatataaata aaatataaaa tatg
//
