LOCUS       NM_152415               4519 bp    mRNA    linear   PRI 08-OCT-2024
DEFINITION  Homo sapiens VPS37A subunit of ESCRT-I (VPS37A), transcript variant
            1, mRNA.
ACCESSION   NM_152415
VERSION     NM_152415.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4519)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   2  (bases 1 to 4519)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   3  (bases 1 to 4519)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   4  (bases 1 to 4519)
  AUTHORS   Flower,T.G., Takahashi,Y., Hudait,A., Rose,K., Tjahjono,N.,
            Pak,A.J., Yokom,A.L., Liang,X., Wang,H.G., Bouamr,F., Voth,G.A. and
            Hurley,J.H.
  TITLE     A helical assembly of human ESCRT-I scaffolds reverse-topology
            membrane scission
  JOURNAL   Nat Struct Mol Biol 27 (6), 570-580 (2020)
   PUBMED   32424346
REFERENCE   5  (bases 1 to 4519)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   6  (bases 1 to 4519)
  AUTHORS   Stuchell,M.D., Garrus,J.E., Muller,B., Stray,K.M., Ghaffarian,S.,
            McKinnon,R., Krausslich,H.G., Morham,S.G. and Sundquist,W.I.
  TITLE     The human endosomal sorting complex required for transport
            (ESCRT-I) and its role in HIV-1 budding
  JOURNAL   J Biol Chem 279 (34), 36059-36071 (2004)
   PUBMED   15218037
REFERENCE   7  (bases 1 to 4519)
  AUTHORS   Xu,Z., Liang,L., Wang,H., Li,T. and Zhao,M.
  TITLE     HCRP1, a novel gene that is downregulated in hepatocellular
            carcinoma, encodes a growth-inhibitory protein
  JOURNAL   Biochem Biophys Res Commun 311 (4), 1057-1066 (2003)
   PUBMED   14623289
  REMARK    GeneRIF: Our results strongly suggest that HCRP1 might be a growth
            inhibitory protein and associated with decreasing the invasion of
            HCC cells
REFERENCE   8  (bases 1 to 4519)
  AUTHORS   Lippincott-Schwartz,J., Roberts,T.H. and Hirschberg,K.
  TITLE     Secretory protein trafficking and organelle dynamics in living
            cells
  JOURNAL   Annu Rev Cell Dev Biol 16, 557-589 (2000)
   PUBMED   11031247
  REMARK    Review article
REFERENCE   9  (bases 1 to 4519)
  AUTHORS   Imafuku,I., Waragai,M., Takeuchi,S., Kanazawa,I., Kawabata,M.,
            Mouradian,M.M. and Okazawa,H.
  TITLE     Polar amino acid-rich sequences bind to polyglutamine tracts
  JOURNAL   Biochem Biophys Res Commun 253 (1), 16-20 (1998)
   PUBMED   9875212
REFERENCE   10 (bases 1 to 4519)
  AUTHORS   Hedera,P.
  TITLE     Hereditary Spastic Paraplegia Overview
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301682
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC091050.3, AY033079.2 and
            AC124074.2.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_152415.2.
            
            Summary: This gene belongs to the VPS37 family, and encodes a
            component of the ESCRT-I (endosomal sorting complex required for
            transport I) protein complex, required for the sorting of
            ubiquitinated transmembrane proteins into internal vesicles of
            multivesicular bodies. Expression of this gene is downregulated in
            hepatocellular carcinoma, and mutations in this gene are associated
            with autosomal recessive spastic paraplegia-53. A related
            pseudogene has been identified on chromosome 5. Alternatively
            spliced transcript variants have been found for this gene.
            [provided by RefSeq, Dec 2012].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660807.199984.1,
                                           SRR1660807.114143.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000324849.9/ ENSP00000318629.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-136               AC091050.3         4908-5043           c
            137-2053            AY033079.2         1-1917
            2054-4519           AC124074.2         153403-155868       c
FEATURES             Location/Qualifiers
     source          1..4519
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="8"
                     /map="8p22"
     gene            1..4519
                     /gene="VPS37A"
                     /gene_synonym="HCRP1; PQBP2; SPG53"
                     /note="VPS37A subunit of ESCRT-I"
                     /db_xref="GeneID:137492"
                     /db_xref="HGNC:HGNC:24928"
                     /db_xref="MIM:609927"
     exon            1..412
                     /gene="VPS37A"
                     /gene_synonym="HCRP1; PQBP2; SPG53"
                     /inference="alignment:Splign:2.1.0"
     CDS             288..1481
                     /gene="VPS37A"
                     /gene_synonym="HCRP1; PQBP2; SPG53"
                     /note="isoform 1 is encoded by transcript variant 1;
                     polyglutamine binding protein 2; vacuolar protein
                     sorting-associated protein 37A; ESCRT-I complex subunit
                     VPS37A; hepatocellular carcinoma-related protein 1;
                     vacuolar protein sorting 37 homolog A; VPS37A, ESCRT-I
                     subunit"
                     /codon_start=1
                     /product="vacuolar protein sorting-associated protein 37A
                     isoform 1"
                     /protein_id="NP_689628.2"
                     /db_xref="CCDS:CCDS6001.1"
                     /db_xref="GeneID:137492"
                     /db_xref="HGNC:HGNC:24928"
                     /db_xref="MIM:609927"
                     /translation="MSWLFPLTKSASSSAAGSPGGLTSLQQQKQRLIESLRNSHSSIA
                     EIQKDVEYRLPFTINNLTININILLPPQFPQEKPVISVYPPIRHHLMDKQGVYVTSPL
                     VNNFTMHSDLGKIIQSLLDEFWKNPPVLAPTSTAFPYLYSNPSGMSPYASQGFPFLPP
                     YPPQEANRSITSLSVADTVSSSTTSHTTAKPAAPSFGVLSNLPLPIPTVDASIPTSQN
                     GFGYKMPDVPDAFPELSELSVSQLTDMNEQEEVLLEQFLTLPQLKQIITDKDDLVKSI
                     EELARKNLLLEPSLEAKRQTVLDKYELLTQMKSTFEKKMQRQHELSESCSASALQARL
                     KVAAHEAEEESDNIAEDFLEGKMEIDDFLSSFMEKRTICHCRRAKEEKLQQAIAMHSQ
                     FHAPL"
     exon            413..487
                     /gene="VPS37A"
                     /gene_synonym="HCRP1; PQBP2; SPG53"
                     /inference="alignment:Splign:2.1.0"
     exon            488..602
                     /gene="VPS37A"
                     /gene_synonym="HCRP1; PQBP2; SPG53"
                     /inference="alignment:Splign:2.1.0"
     exon            603..703
                     /gene="VPS37A"
                     /gene_synonym="HCRP1; PQBP2; SPG53"
                     /inference="alignment:Splign:2.1.0"
     exon            704..929
                     /gene="VPS37A"
                     /gene_synonym="HCRP1; PQBP2; SPG53"
                     /inference="alignment:Splign:2.1.0"
     exon            930..1000
                     /gene="VPS37A"
                     /gene_synonym="HCRP1; PQBP2; SPG53"
                     /inference="alignment:Splign:2.1.0"
     exon            1001..1128
                     /gene="VPS37A"
                     /gene_synonym="HCRP1; PQBP2; SPG53"
                     /inference="alignment:Splign:2.1.0"
     exon            1129..1187
                     /gene="VPS37A"
                     /gene_synonym="HCRP1; PQBP2; SPG53"
                     /inference="alignment:Splign:2.1.0"
     exon            1188..1256
                     /gene="VPS37A"
                     /gene_synonym="HCRP1; PQBP2; SPG53"
                     /inference="alignment:Splign:2.1.0"
     exon            1257..1400
                     /gene="VPS37A"
                     /gene_synonym="HCRP1; PQBP2; SPG53"
                     /inference="alignment:Splign:2.1.0"
     exon            1401..1481
                     /gene="VPS37A"
                     /gene_synonym="HCRP1; PQBP2; SPG53"
                     /inference="alignment:Splign:2.1.0"
     exon            1482..4519
                     /gene="VPS37A"
                     /gene_synonym="HCRP1; PQBP2; SPG53"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2033..2038
                     /regulatory_class="polyA_signal_sequence"
                     /gene="VPS37A"
                     /gene_synonym="HCRP1; PQBP2; SPG53"
                     /note="hexamer: AATAAA"
     polyA_site      2054
                     /gene="VPS37A"
                     /gene_synonym="HCRP1; PQBP2; SPG53"
                     /note="major polyA site"
     regulatory      4493..4498
                     /regulatory_class="polyA_signal_sequence"
                     /gene="VPS37A"
                     /gene_synonym="HCRP1; PQBP2; SPG53"
                     /note="hexamer: AATAAA"
     polyA_site      4519
                     /gene="VPS37A"
                     /gene_synonym="HCRP1; PQBP2; SPG53"
ORIGIN      
        1 gctgggcggc caggctccct ggctggccgg tttgggcgtc tgggccgtga aggtgggacc
       61 tcctgttccg ggccgcaagt ttccctctcc agccgcccgc cgttcgtagc atgtccccca
      121 gaactcgggg agcgcaggca ggacaggctt agagaagacg cggtccccag cgcttgggcc
      181 acggacgtcc caccccgctc ctctgtcgct ggagaaccgc cgggccgagc cactgggaga
      241 agcaggccag agccttccag ggcctccggc ccgtggaccc gaggaggatg agctggcttt
      301 ttcccctgac caagagcgcc tcctcctccg cggctgggtc ccccggtggc ctcaccagcc
      361 tccagcagca gaagcagcgc ctgatcgagt ccctccggaa ctcacactcc agtatagccg
      421 aaatacagaa agatgtggaa tacagattgc cattcaccat aaacaacctg acaattaaca
      481 ttaatatatt gcttcctcca cagtttcctc aggaaaaacc agtgatcagt gtttatccac
      541 caatacgaca tcacttaatg gataaacaag gagtgtatgt tacctctcca ttagtaaaca
      601 attttacaat gcactcagat cttggaaaaa ttattcagag tctgttggat gagttttgga
      661 agaatcctcc agttttagct cctacttcaa cagcatttcc ttatctatac agtaacccaa
      721 gtgggatgtc tccttatgct tctcagggtt ttccatttct tcctccatat cctccacaag
      781 aagcaaacag gagtatcact tctttatctg ttgctgacac tgtttcttct tcaacaacaa
      841 gtcataccac agccaagcct gccgctcctt catttggtgt cctttcaaat ctgccattac
      901 ccattcccac agtggatgct tcaataccga caagccaaaa tggttttggg tacaagatgc
      961 cagatgtccc tgatgcattt ccagaactct cagaactaag tgtgtcacaa ctcacagata
     1021 tgaatgaaca agaggaggta ttactagaac agtttctgac tttgcctcaa ctaaaacaaa
     1081 ttattaccga caaagatgac ttagtaaaaa gtattgagga actagcaaga aaaaatctcc
     1141 ttttggagcc cagcttggaa gccaaaagac aaactgtttt agataagtat gaattactta
     1201 cacagatgaa gtccactttc gaaaagaaga tgcaaaggca gcatgaactt agtgagagct
     1261 gtagtgcaag tgcccttcag gcaagattga aagtagctgc acatgaagct gaggaagaat
     1321 ctgataatat tgcagaagac ttcttggagg gaaagatgga aatagatgat tttctcagta
     1381 gcttcatgga aaagagaaca atttgccact gtagaagagc caaggaagag aaacttcagc
     1441 aggcgatagc aatgcacagc caatttcatg ctccactata gattttcctg gaaacatgaa
     1501 ctgccaagag aggaatggga cacaaaacca aacactgttt tatatttatg gtttgcaaac
     1561 tggcatttca tcagtggcta aattcacaga tatcctatat agattgtata cagaactgag
     1621 actgattttg taccgattag aatgattgct atgatctttg agaaattttt ctgcactatt
     1681 tgcactgaaa tgtttattta ttgttgataa attgtatcat atttaagttc cactgctgtt
     1741 cctcttacct tgattaaatg cctatgcatg tacttttagc tagtttttaa tattttataa
     1801 aacttcattt aaatttgtat ttttaacttg aagttccatt tctttatcaa ggatggtatt
     1861 tagatttttt tcctcttaac cttttttcaa aaactatttt caactgtgag gaaaccctta
     1921 tttttctttc tttgtggata aaactttcaa aagcaattta agatattcat agtgttagga
     1981 aacaccaaac ctgcctatgt gccatctcac aaaagaaact tttaatacct acaataaatc
     2041 aaaagaataa accagctgtt cttatatatt gtttcatttt taaaactaaa gatgcattta
     2101 agaagcaata caagtaaata ttttacctaa taggaaaaaa aaaagttgcc tttcatttaa
     2161 accattccaa cagaaattct tatgctaatt taaaacatat atatatctgg taggtttgtg
     2221 gttggatagg ttttctaaat tcctaatgtt aaaaacaatc tttatgttaa tatacactaa
     2281 atctatacac aaaaaaagtc agtgaacttt tctgaccttt actgtgagtt accttttcct
     2341 aagaggaaag ctatagtaat aagtaaaatt taatttttag gcaatcctga tttttaatga
     2401 atttaattga gtgttcttgt atactacatt gagcagtttg cttctatacc gtgtcacaaa
     2461 attcatgtat ttcttgagaa gccctaaaag ctcataaagg aaaatgccgt gaactatgta
     2521 gctcaggctt ggtaaggtgc catctaaatt acaaaacaaa ctaatgcata attttgctta
     2581 aatttcatcc cagtatgatt gtcttcccaa caccagcata tagtatagat tgtctgtctt
     2641 ttttatattt tttagttctt cctgtacatg tttttggcaa taaagttata ggaagaacaa
     2701 aattattttg ttagaattaa aacatgctta atatttagtc tgtttgtgga gggcaggtat
     2761 tcacgtggac tgagatacaa tgttggatac agaaaataac tttcattgtc ttcctgacac
     2821 tgtgctaagg acatgctgtt aaagcttcaa agtgaccaga tgaggaagga ataattaatt
     2881 attactcctg atttgtagat aactgaggta agagtgtttc aaatttatga tagtcttttg
     2941 ggtattcaga aacctttcct tatactgcac tggccaccag agcttaattt tcccagcagt
     3001 tacagcaatg ggagatagaa cagtctcaat cttttgccaa ccatcaggtt cctagaaacc
     3061 aggtaggtgt atcccataac aagggaggag cataccacag cccctcattt gattaattca
     3121 tttgatctat ctatgttatt aagtacctac taggaataag gcattgtgga aatactatac
     3181 aaagataaac attgtttaga tgcttatcta ctttcctttt caccagaaaa acagaaaaaa
     3241 aagaaacatt ttcttacaga gtaaaaatgt tctacataat cacatgagta gttcatctca
     3301 gtgtttttta ttctttaaag ttgaactatc ccagtttcat tctataccat tcattggata
     3361 accttgttac aacccagtca tgaaacagag cagtgtgatc agttatctgc atttaacaaa
     3421 tagacaaatc agtttacata aaggttatgt atgtcaccca cgatgaaaag aatctgcatt
     3481 tgaatatgcc cgtatgaatg tgggttctgt ttttgcaaca gagattaagt gaccattttt
     3541 tctaatttta tggctatata ttttcttcat aaaaattggt cacatcggag aagcagtgcc
     3601 acaggaaaaa tgaaatgcat gtgaaagttt gtattctgat tttacaagat gagatagaaa
     3661 tcagaattaa agaggaatac ttaggagtta ctaggctaat cagtgtacga atttgtcata
     3721 ggtagagatt taaaggttaa tatcttaaaa tagaagaaaa ttctaaatca atcaatcagt
     3781 gagatataaa ctaaacagac ccacttcaaa gttgaaagaa atttctaggc ataaattgag
     3841 actaggaaat ttatatcaga atagagggtg cttgacacat atatatgctt aaattgaagg
     3901 acagctcaga ttcattttta ggagaagaaa gtaaactaat gtgctcttaa agaataaaaa
     3961 tttattctat ggtttctgtc tctgatcatc accttccatt ctataaaaag ctcagttact
     4021 gatttgctgg gtcatggtca aaattcttac ctatttattt catatcaact ttaaaaaata
     4081 aattacttgc attctatata ttactaattg ggaagtaata tgcctcaaat cagttttata
     4141 ctggattatt ccctatgctt taaaccactg ctctcaataa aacacttcct gattaatgtt
     4201 tgattattag atattttagt cttgttgggg atattttagt cttgttgggt tagccatgct
     4261 ctgaagaatc tgtgaaagta cagtaaagtt ttaataagca ataaatgtaa ccttttatat
     4321 aaatctcagt gctaggttaa cttctaataa gcagacgaac atgttacata aattataatg
     4381 tctgtcttgt aaaaaagttg aggggactaa aagtttatga ctctgatatg gaagttgtca
     4441 tattaaaaaa ctacatttta aaacatcaaa tatttatact atttgctttt caaataaaag
     4501 catagtgctg tttggcata
//
