LOCUS       NM_016075               4425 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens vacuolar protein sorting 36 homolog (VPS36),
            transcript variant 1, mRNA.
ACCESSION   NM_016075
VERSION     NM_016075.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4425)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   2  (bases 1 to 4425)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   3  (bases 1 to 4425)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   4  (bases 1 to 4425)
  AUTHORS   Kaewborisuth,C., Yingchutrakul,Y., Roytrakul,S. and
            Jongkaewwattana,A.
  TITLE     Porcine Epidemic Diarrhea Virus (PEDV) ORF3 Interactome Reveals
            Inhibition of Virus Replication by Cellular VPS36 Protein
  JOURNAL   Viruses 11 (4), 382 (2019)
   PUBMED   31022991
  REMARK    GeneRIF: As a result of VPS36-ORF3 interaction, Porcine Epidemic
            Diarrhea Virus replication was substantially suppressed in cells
            overexpressing VPS36.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 4425)
  AUTHORS   Im,Y.J. and Hurley,J.H.
  TITLE     Integrated structural model and membrane targeting mechanism of the
            human ESCRT-II complex
  JOURNAL   Dev Cell 14 (6), 902-913 (2008)
   PUBMED   18539118
REFERENCE   6  (bases 1 to 4425)
  AUTHORS   Wang,T. and Hong,W.
  TITLE     RILP interacts with VPS22 and VPS36 of ESCRT-II and regulates their
            membrane recruitment
  JOURNAL   Biochem Biophys Res Commun 350 (2), 413-423 (2006)
   PUBMED   17010938
REFERENCE   7  (bases 1 to 4425)
  AUTHORS   Langelier,C., von Schwedler,U.K., Fisher,R.D., De Domenico,I.,
            White,P.L., Hill,C.P., Kaplan,J., Ward,D. and Sundquist,W.I.
  TITLE     Human ESCRT-II complex and its role in human immunodeficiency virus
            type 1 release
  JOURNAL   J Virol 80 (19), 9465-9480 (2006)
   PUBMED   16973552
REFERENCE   8  (bases 1 to 4425)
  AUTHORS   Bowers,K., Piper,S.C., Edeling,M.A., Gray,S.R., Owen,D.J.,
            Lehner,P.J. and Luzio,J.P.
  TITLE     Degradation of endocytosed epidermal growth factor and virally
            ubiquitinated major histocompatibility complex class I is
            independent of mammalian ESCRTII
  JOURNAL   J Biol Chem 281 (8), 5094-5105 (2006)
   PUBMED   16371348
  REMARK    GeneRIF: mammalian ESCRTII is made up of hVps25p, hVps22p, and
            hVps36p and may be redundant, cargo-specific, or not required for
            protein sorting at the multivesicular body
REFERENCE   9  (bases 1 to 4425)
  AUTHORS   Slagsvold,T., Aasland,R., Hirano,S., Bache,K.G., Raiborg,C.,
            Trambaiolo,D., Wakatsuki,S. and Stenmark,H.
  TITLE     Eap45 in mammalian ESCRT-II binds ubiquitin via a
            phosphoinositide-interacting GLUE domain
  JOURNAL   J Biol Chem 280 (20), 19600-19606 (2005)
   PUBMED   15755741
REFERENCE   10 (bases 1 to 4425)
  AUTHORS   Kamura,T., Burian,D., Khalili,H., Schmidt,S.L., Sato,S., Liu,W.J.,
            Conrad,M.N., Conaway,R.C., Conaway,J.W. and Shilatifard,A.
  TITLE     Cloning and characterization of ELL-associated proteins EAP45 and
            EAP20. a role for yeast EAP-like proteins in regulation of gene
            expression by glucose
  JOURNAL   J Biol Chem 276 (19), 16528-16533 (2001)
   PUBMED   11278625
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA461148.1, AK289740.1,
            BC037279.1 and AI352102.1.
            
            On Nov 22, 2018 this sequence version replaced NM_016075.3.
            
            Summary: This gene encodes a protein that is a subunit of the
            endosomal sorting complex required for transport II (ESCRT-II).
            This protein complex functions in sorting of ubiquitinated membrane
            proteins during endocytosis. A similar protein complex in rat is
            associated with RNA polymerase elongation factor II. [provided by
            RefSeq, Aug 2013].
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660803.16788.1,
                                           SRR1660807.127893.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000378060.9/ ENSP00000367299.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-13                DA461148.1         45-57
            14-1641             AK289740.1         1-1628
            1642-4327           BC037279.1         1635-4320
            4328-4425           AI352102.1         9-106               c
FEATURES             Location/Qualifiers
     source          1..4425
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="13"
                     /map="13q14.3"
     gene            1..4425
                     /gene="VPS36"
                     /gene_synonym="C13orf9; CGI-145; EAP45"
                     /note="vacuolar protein sorting 36 homolog"
                     /db_xref="GeneID:51028"
                     /db_xref="HGNC:HGNC:20312"
                     /db_xref="MIM:610903"
     exon            1..136
                     /gene="VPS36"
                     /gene_synonym="C13orf9; CGI-145; EAP45"
                     /inference="alignment:Splign:2.1.0"
     CDS             41..1201
                     /gene="VPS36"
                     /gene_synonym="C13orf9; CGI-145; EAP45"
                     /note="isoform 1 is encoded by transcript variant 1;
                     vacuolar protein-sorting-associated protein 36; ESCRT-II
                     complex subunit VPS36; ELL-associated protein of 45 kDa"
                     /codon_start=1
                     /product="vacuolar protein-sorting-associated protein 36
                     isoform 1"
                     /protein_id="NP_057159.2"
                     /db_xref="CCDS:CCDS9434.1"
                     /db_xref="GeneID:51028"
                     /db_xref="HGNC:HGNC:20312"
                     /db_xref="MIM:610903"
                     /translation="MDRFVWTSGLLEINETLVIQQRGVRIYDGEEKIKFDAGTLLLST
                     HRLIWRDQKNHECCMAILLSQIVFIEEQAAGIGKSAKIVVHLHPAPPNKEPGPFQSSK
                     NSYIKLSFKEHGQIEFYRRLSEEMTQRRWENMPVSQSLQTNRGPQPGRIRAVGIVGIE
                     RKLEEKRKETDKNISEAFEDLSKLMIKAKEMVELSKSIANKIKDKQGDITEDETIRFK
                     SYLLSMGIANPVTRETYGSGTQYHMQLAKQLAGILQVPLEERGGIMSLTEVYCLVNRA
                     RGMELLSPEDLVNACKMLEALKLPLRLRVFDSGVMVIELQSHKEEEMVASALETVSEK
                     GSLTSEEFAKLVGMSVLLAKERLLLAEKMGHLCRDDSVEGLRFYPNLFMTQS"
     exon            137..205
                     /gene="VPS36"
                     /gene_synonym="C13orf9; CGI-145; EAP45"
                     /inference="alignment:Splign:2.1.0"
     exon            206..276
                     /gene="VPS36"
                     /gene_synonym="C13orf9; CGI-145; EAP45"
                     /inference="alignment:Splign:2.1.0"
     exon            277..391
                     /gene="VPS36"
                     /gene_synonym="C13orf9; CGI-145; EAP45"
                     /inference="alignment:Splign:2.1.0"
     exon            392..481
                     /gene="VPS36"
                     /gene_synonym="C13orf9; CGI-145; EAP45"
                     /inference="alignment:Splign:2.1.0"
     exon            482..568
                     /gene="VPS36"
                     /gene_synonym="C13orf9; CGI-145; EAP45"
                     /inference="alignment:Splign:2.1.0"
     exon            569..601
                     /gene="VPS36"
                     /gene_synonym="C13orf9; CGI-145; EAP45"
                     /inference="alignment:Splign:2.1.0"
     exon            602..679
                     /gene="VPS36"
                     /gene_synonym="C13orf9; CGI-145; EAP45"
                     /inference="alignment:Splign:2.1.0"
     exon            680..814
                     /gene="VPS36"
                     /gene_synonym="C13orf9; CGI-145; EAP45"
                     /inference="alignment:Splign:2.1.0"
     exon            815..880
                     /gene="VPS36"
                     /gene_synonym="C13orf9; CGI-145; EAP45"
                     /inference="alignment:Splign:2.1.0"
     exon            881..945
                     /gene="VPS36"
                     /gene_synonym="C13orf9; CGI-145; EAP45"
                     /inference="alignment:Splign:2.1.0"
     exon            946..1030
                     /gene="VPS36"
                     /gene_synonym="C13orf9; CGI-145; EAP45"
                     /inference="alignment:Splign:2.1.0"
     exon            1031..1107
                     /gene="VPS36"
                     /gene_synonym="C13orf9; CGI-145; EAP45"
                     /inference="alignment:Splign:2.1.0"
     exon            1108..4425
                     /gene="VPS36"
                     /gene_synonym="C13orf9; CGI-145; EAP45"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      3881
                     /gene="VPS36"
                     /gene_synonym="C13orf9; CGI-145; EAP45"
                     /note="major polyA site"
     regulatory      4307..4312
                     /regulatory_class="polyA_signal_sequence"
                     /gene="VPS36"
                     /gene_synonym="C13orf9; CGI-145; EAP45"
                     /note="hexamer: AATAAA"
     polyA_site      4331
                     /gene="VPS36"
                     /gene_synonym="C13orf9; CGI-145; EAP45"
     regulatory      4397..4402
                     /regulatory_class="polyA_signal_sequence"
                     /gene="VPS36"
                     /gene_synonym="C13orf9; CGI-145; EAP45"
                     /note="hexamer: AATAAA"
     polyA_site      4425
                     /gene="VPS36"
                     /gene_synonym="C13orf9; CGI-145; EAP45"
ORIGIN      
        1 gctcgcggcc tcggagggcc ggggctgggt ggcagcggcc atggaccgct tcgtttggac
       61 cagcggcctc ctggagatca acgagaccct ggtgatccag cagcgcgggg tgcgaatcta
      121 cgatggcgag gagaagataa aatttgatgc tgggactctc cttcttagta cacaccgact
      181 gatttggaga gatcagaaaa atcatgagtg ttgcatggcc attctccttt cccaaattgt
      241 gttcattgaa gaacaggcgg ctggaattgg gaagagtgcc aaaatagtgg ttcatcttca
      301 cccagctcct cctaacaaag aacctggccc attccagagt agtaagaact cctacatcaa
      361 actctccttc aaagaacatg gccagattga gttttacagg cgtttatcag aggaaatgac
      421 acaaagaaga tgggagaata tgccagtttc ccagtcatta caaacaaata gaggacccca
      481 gccaggaaga ataagggctg taggaattgt aggtattgaa aggaaactgg aagaaaaaag
      541 aaaagaaact gacaaaaaca tttctgaggc ctttgaagac ctcagcaaac taatgatcaa
      601 ggctaaggaa atggtggaat tatcaaaatc aattgctaat aaaattaaag acaaacaagg
      661 tgacatcaca gaagatgaga ccatcaggtt taaatcctac ttgctgagca tgggaatagc
      721 taacccagtt accagagaaa cctacggctc aggcacacag taccacatgc agctggccaa
      781 acaactggct ggaatattgc aggtgccttt agaggaacga gggggaataa tgtcactcac
      841 ggaggtgtac tgcttagtaa accgagctcg aggaatggaa ttgctctcac cagaagattt
      901 agtgaatgcg tgcaagatgc tggaagcact gaaattacct ctcaggctcc gtgtgtttga
      961 cagtggcgtc atggtaattg agcttcagtc tcacaaggaa gaggaaatgg tggcctcggc
     1021 cctggagaca gtttcagaaa agggatccct aacatcagaa gagtttgcta agcttgtggg
     1081 aatgtctgtc ctcctagcca aagaaaggtt gctgcttgca gagaagatgg gccatctttg
     1141 ccgtgatgac tcagtggaag gcctgcgttt ttacccaaat ttatttatga cacagagcta
     1201 agggttttgt atttaaaatc ctttttgtcc atatgcttgc gtcatgtaga ggttgtatga
     1261 cattgagcta agagataaac cccgatcaat tgagaattta ttggaacttc acagtgcaat
     1321 gtaaatctct tttaatttct cactaaatat ggtccaggaa atttatttag tatacgcata
     1381 ggaaaattca gaaaagtgaa tgccaatatg aatttaaaat catgctatag tgcagaaccc
     1441 tcagagttta acttggaata tagtggattt taacttgatc ctcaaatcta atcattttat
     1501 aaagaaggga atttagtttt gcagagaata aaaagagaag ttgcatgttc agacaggtta
     1561 gattattatt ttggtgtaac tgaaattcac tgattgcaca tgacaatgtt gggacaaaat
     1621 atactgcagc atgctatatg aggctcctcc ccagggcttt tagaagcagt catagacatg
     1681 tcttcaacat accaaataaa atacctttaa aaatgaaata attttatttg acacatatat
     1741 ttatatatat tctatctagt ttctctttgt ttttttaagt gatgatttca tgactggcat
     1801 ttaaagaatg caactgtgtc attttgtttc caaatgctgt ggattttgaa actgaactag
     1861 agagctgtat agacatgccc agagttatga ttacaaattt aggaggtaga cggctcagga
     1921 attccctggg attgttgtgc tggtggaatg gcagagggaa cttcacagga accttagtgt
     1981 tcttttacct caaagccaca gacaggaaat agaaagtgga aaagtaatat ctccttttct
     2041 tttccataag gagtttcaac actgaacttt aaaaagtcta tcatattcca gcaatatttt
     2101 ttctttgtcc tttatgttgt aagttgtgtg gaaaaactac ttcggtaaga aatgttactg
     2161 agataacaac aactggctaa tactgcatgt agattgctta ggttttaaag tgactgcctg
     2221 acttcacatg ttattgctac agcctccagt atgttcgcat tatctcaaac tcagggaccc
     2281 cacaggacag gagacaccct ttctgaaact gagttggaag tgaaagggtg gtgatggttt
     2341 tggccaagcc tgcgggaggg aaagtattgt attgggagca cccttgggac caggaagagg
     2401 gatgcccagg ttcacactct gggaccccta agacattgtc agtgggtaag gtggagggcc
     2461 actgccagag gctgctgcag tgccctctgg gaagtgccag ggaccctcag ctgcagaccc
     2521 aggggatccc aatcccttat ctctgcctga aaagtggcta ttgctgcttc gtatcctcca
     2581 gtgtcacagg aatgctgtgc ctgagacatc ccactttagt ttttgttttc caaacctgtc
     2641 actgactctt cattgtggtg acagatcctc tggttccccc attgctcagc ctcaggttta
     2701 ttttgagggt aactcagtgt ctgactgccc aagtctttta ggggttagtt ggaccattgt
     2761 gtaagcttgt ttgtatgtct cacctctttc tggttggtac ttactgtctc acactgctct
     2821 ggcaaacttg tacacacaca cacactctct ctctctctca cctggctaag gcttttaaaa
     2881 ttatgaagat aaataatctg tttcaccagc tggagtgagt tgcaaggaag attgctggag
     2941 ctactcaatc tagcgctaat ggtttggatt cattactgca aacctacata atttaacata
     3001 tttgtttact ttagtgtgac aactgatgaa aaaaaatgga gcaatctgaa ttgtataaaa
     3061 taacttaaga aggaagaaaa gtgatatata aatatatttt gcaaatgtca cattaattta
     3121 aaaatgagta tgattgattt tatttttaaa gtgggcattc ttcactgttt cgagaccttt
     3181 gtatgtattt gtgtattttt atcttttttt ttcaggccat tattataagg tgttattttg
     3241 gccctctaat gtagaagtta tgtttaaata caaccaatca ggccctaagt gcaaaaaagt
     3301 tctgtcttgg tgcttatcac tggtataatt attatttttt tgttttccta actttgctct
     3361 taggagcatg agcctctttg tagctttatg gtaatgcaat atttctggtc atttactgtc
     3421 aaaaaatttt tttacattgt gttagtgaag actgtgtttt cagggttaaa tgattggtat
     3481 caatgtatat agagtaaaat tattgcaaaa tttaaaaatg atttttcttg gcattctttt
     3541 taaatactct gaaacatata tgcaaatgag aaacctttaa tcatactaaa gccagttatg
     3601 ttaagaattt ttcctttgga ttttataatt aagagttgct tataaatact tatgacattg
     3661 agctcttact gttttagttg tcttaaaaca atgggacatg tcatgaactg gtttcttttt
     3721 ttaaaaaatt cttgatgtga attccactgt tacctgacca tgtatattca gatgaatgta
     3781 ctctttcttg ttccttgtta ccagattaat ctgagagaat gtggattcat cctgtcatct
     3841 ttctcatact caaatactta aaatggttct tttgcccaaa agtctcagag tcttcatttt
     3901 ggtacagtgg agtcacaact tgtaaggtgg ttaggtttta aagaccgtgc ctaaggacaa
     3961 aattgagcat gctcagaatg gtccttgaaa atctcctaaa ttaccaataa atttctcttt
     4021 gtagtcttaa agtttaaatc cctgtggagt gatatgtagg tatgaatata ttatgcttat
     4081 attaagtaca aaaatacaaa ctgcatatca agagattctt atagcattaa taatttccat
     4141 gcatgtgtct ttttccagta ggtatggttg aatttatgta aatttattgc taatcccatc
     4201 ccttacgatc tagagtataa gctgcgcaag ggcagaagtt tttatctggt ttgttcatgg
     4261 atgtattcta agagctgaga acagggcctg gacacaataa gcattcaata aatatttact
     4321 gaatgaatga actcctacct atattcctat ttataatttg gctccacttt atcctacttt
     4381 agctcccatt caattcaata aaaaaaacat ttttttgaac acata
//
