LOCUS       NM_018206               6776 bp    mRNA    linear   PRI 11-OCT-2024
DEFINITION  Homo sapiens VPS35 retromer complex component (VPS35), mRNA.
ACCESSION   NM_018206
VERSION     NM_018206.6
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6776)
  AUTHORS   Rowlands,J. and Moore,D.J.
  TITLE     VPS35 and retromer dysfunction in Parkinson's disease
  JOURNAL   Philos Trans R Soc Lond B Biol Sci 379 (1899), 20220384 (2024)
   PUBMED   38368930
  REMARK    GeneRIF: VPS35 and retromer dysfunction in Parkinson's disease.
            Review article
REFERENCE   2  (bases 1 to 6776)
  AUTHORS   Shiraishi,T., Bono,K., Hiraki,H., Manome,Y., Oka,H., Iguchi,Y. and
            Okano,H.J.
  TITLE     The impact of VPS35 D620N mutation on alternative autophagy and its
            reversal by estrogen in Parkinson's disease
  JOURNAL   Cell Mol Life Sci 81 (1), 103 (2024)
   PUBMED   38409392
  REMARK    GeneRIF: The impact of VPS35 D620N mutation on alternative
            autophagy and its reversal by estrogen in Parkinson's disease.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 6776)
  AUTHORS   Zhou,Q., Qi,F., Zhou,C., Ji,J., Jiang,J., Wang,C., Zhao,Q., Jin,Y.,
            Wu,J., Cai,Q., Tian,H. and Zhang,J.
  TITLE     VPS35 promotes gastric cancer progression through integrin/FAK/SRC
            signalling-mediated IL-6/STAT3 pathway activation in a
            YAP-dependent manner
  JOURNAL   Oncogene 43 (2), 106-122 (2024)
   PUBMED   37950040
  REMARK    GeneRIF: VPS35 promotes gastric cancer progression through
            integrin/FAK/SRC signalling-mediated IL-6/STAT3 pathway activation
            in a YAP-dependent manner.
REFERENCE   4  (bases 1 to 6776)
  AUTHORS   Healy,M.D., McNally,K.E., Butkovic,R., Chilton,M., Kato,K.,
            Sacharz,J., McConville,C., Moody,E.R.R., Shaw,S.,
            Planelles-Herrero,V.J., Yadav,S.K.N., Ross,J., Borucu,U.,
            Palmer,C.S., Chen,K.E., Croll,T.I., Hall,R.J., Caruana,N.J.,
            Ghai,R., Nguyen,T.H.D., Heesom,K.J., Saitoh,S., Berger,I.,
            Schaffitzel,C., Williams,T.A., Stroud,D.A., Derivery,E.,
            Collins,B.M. and Cullen,P.J.
  TITLE     Structure of the endosomal Commander complex linked to
            Ritscher-Schinzel syndrome
  JOURNAL   Cell 186 (10), 2219-2237 (2023)
   PUBMED   37172566
REFERENCE   5  (bases 1 to 6776)
  AUTHORS   Zhang,P., Yu,L., Gao,J., Fu,Q., Dai,F., Zhao,Y., Zheng,L. and
            Zhao,S.
  TITLE     Cloning and characterization of human VPS35 and mouse Vps35 and
            mapping of VPS35 to human chromosome 16q13-q21
  JOURNAL   Genomics 70 (2), 253-257 (2000)
   PUBMED   11112353
REFERENCE   6  (bases 1 to 6776)
  AUTHORS   Haft,C.R., de la Luz Sierra,M., Bafford,R., Lesniak,M.A., Barr,V.A.
            and Taylor,S.I.
  TITLE     Human orthologs of yeast vacuolar protein sorting proteins Vps26,
            29, and 35: assembly into multimeric complexes
  JOURNAL   Mol Biol Cell 11 (12), 4105-4116 (2000)
   PUBMED   11102511
REFERENCE   7  (bases 1 to 6776)
  AUTHORS   Edgar,A.J. and Polak,J.M.
  TITLE     Human homologues of yeast vacuolar protein sorting 29 and 35
  JOURNAL   Biochem Biophys Res Commun 277 (3), 622-630 (2000)
   PUBMED   11062004
REFERENCE   8  (bases 1 to 6776)
  AUTHORS   Simpson,J.C., Wellenreuther,R., Poustka,A., Pepperkok,R. and
            Wiemann,S.
  TITLE     Systematic subcellular localization of novel proteins identified by
            large-scale cDNA sequencing
  JOURNAL   EMBO Rep 1 (3), 287-292 (2000)
   PUBMED   11256614
REFERENCE   9  (bases 1 to 6776)
  AUTHORS   Dulski,J., Ross,O.A. and Wszolek,Z.K.
  TITLE     VPS35-Related Parkinson Disease
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   28796472
REFERENCE   10 (bases 1 to 6776)
  AUTHORS   Cook Shukla,L., Schulze,J., Farlow,J., Pankratz,N.D., Wojcieszek,J.
            and Foroud,T.
  TITLE     Parkinson Disease Overview
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301402
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC007225.2 and AC012186.8.
            This sequence is a reference standard in the RefSeqGene project.
            
            On May 17, 2019 this sequence version replaced NM_018206.5.
            
            Summary: This gene belongs to a group of vacuolar protein sorting
            (VPS) genes. The encoded protein is a component of a large
            multimeric complex, termed the retromer complex, involved in
            retrograde transport of proteins from endosomes to the trans-Golgi
            network. The close structural similarity between the yeast and
            human proteins that make up this complex suggests a similarity in
            function. Expression studies in yeast and mammalian cells indicate
            that this protein interacts directly with VPS35, which serves as
            the core of the retromer complex. [provided by RefSeq, Jul 2008].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC093036.1, SRR11853566.10248.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000299138.12/ ENSP00000299138.7
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-48                AC007225.2         196333-196380
            49-147              AC012186.8         160453-160551       c
            148-244             AC012186.8         159024-159120       c
            245-368             AC012186.8         158322-158445       c
            369-551             AC012186.8         157616-157798       c
            552-765             AC012186.8         155888-156101       c
            766-849             AC012186.8         154260-154343       c
            850-959             AC012186.8         153528-153637       c
            960-1056            AC012186.8         151509-151605       c
            1057-1205           AC012186.8         151259-151407       c
            1206-1413           AC012186.8         149210-149417       c
            1414-1569           AC012186.8         148650-148805       c
            1570-1692           AC012186.8         145875-145997       c
            1693-1872           AC012186.8         139928-140107       c
            1873-2112           AC012186.8         139188-139427       c
            2113-2256           AC012186.8         138663-138806       c
            2257-6776           AC012186.8         133077-137596       c
FEATURES             Location/Qualifiers
     source          1..6776
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="16"
                     /map="16q11.2"
     gene            1..6776
                     /gene="VPS35"
                     /gene_synonym="MEM3; PARK17"
                     /note="VPS35 retromer complex component"
                     /db_xref="GeneID:55737"
                     /db_xref="HGNC:HGNC:13487"
                     /db_xref="MIM:601501"
     exon            1..48
                     /gene="VPS35"
                     /gene_synonym="MEM3; PARK17"
                     /inference="alignment:Splign:2.1.0"
     CDS             46..2436
                     /gene="VPS35"
                     /gene_synonym="MEM3; PARK17"
                     /note="maternal-embryonic 3; hVPS35; vacuolar protein
                     sorting 35 homolog"
                     /codon_start=1
                     /product="vacuolar protein sorting-associated protein 35"
                     /protein_id="NP_060676.2"
                     /db_xref="CCDS:CCDS10721.1"
                     /db_xref="GeneID:55737"
                     /db_xref="HGNC:HGNC:13487"
                     /db_xref="MIM:601501"
                     /translation="MPTTQQSPQDEQEKLLDEAIQAVKVQSFQMKRCLDKNKLMDALK
                     HASNMLGELRTSMLSPKSYYELYMAISDELHYLEVYLTDEFAKGRKVADLYELVQYAG
                     NIIPRLYLLITVGVVYVKSFPQSRKDILKDLVEMCRGVQHPLRGLFLRNYLLQCTRNI
                     LPDEGEPTDEETTGDISDSMDFVLLNFAEMNKLWVRMQHQGHSRDREKRERERQELRI
                     LVGTNLVRLSQLEGVNVERYKQIVLTGILEQVVNCRDALAQEYLMECIIQVFPDEFHL
                     QTLNPFLRACAELHQNVNVKNIIIALIDRLALFAHREDGPGIPADIKLFDIFSQQVAT
                     VIQSRQDMPSEDVVSLQVSLINLAMKCYPDRVDYVDKVLETTVEIFNKLNLEHIATSS
                     AVSKELTRLLKIPVDTYNNILTVLKLKHFHPLFEYFDYESRKSMSCYVLSNVLDYNTE
                     IVSQDQVDSIMNLVSTLIQDQPDQPVEDPDPEDFADEQSLVGRFIHLLRSEDPDQQYL
                     ILNTARKHFGAGGNQRIRFTLPPLVFAAYQLAFRYKENSKVDDKWEKKCQKIFSFAHQ
                     TISALIKAELAELPLRLFLQGALAAGEIGFENHETVAYEFMSQAFSLYEDEISDSKAQ
                     LAAITLIIGTFERMKCFSEENHEPLRTQCALAASKLLKKPDQGRAVSTCAHLFWSGRN
                     TDKNGEELHGGKRVMECLKKALKIANQCMDPSLQVQLFIEILNRYIYFYEKENDAVTI
                     QVLNQLIQKIREDLPNLESSEETEQINKHFHNTLEHLRLRRESPESEGPIYEGLIL"
     exon            49..147
                     /gene="VPS35"
                     /gene_synonym="MEM3; PARK17"
                     /inference="alignment:Splign:2.1.0"
     exon            148..244
                     /gene="VPS35"
                     /gene_synonym="MEM3; PARK17"
                     /inference="alignment:Splign:2.1.0"
     exon            245..368
                     /gene="VPS35"
                     /gene_synonym="MEM3; PARK17"
                     /inference="alignment:Splign:2.1.0"
     exon            369..551
                     /gene="VPS35"
                     /gene_synonym="MEM3; PARK17"
                     /inference="alignment:Splign:2.1.0"
     exon            552..765
                     /gene="VPS35"
                     /gene_synonym="MEM3; PARK17"
                     /inference="alignment:Splign:2.1.0"
     exon            766..849
                     /gene="VPS35"
                     /gene_synonym="MEM3; PARK17"
                     /inference="alignment:Splign:2.1.0"
     exon            850..959
                     /gene="VPS35"
                     /gene_synonym="MEM3; PARK17"
                     /inference="alignment:Splign:2.1.0"
     exon            960..1056
                     /gene="VPS35"
                     /gene_synonym="MEM3; PARK17"
                     /inference="alignment:Splign:2.1.0"
     exon            1057..1205
                     /gene="VPS35"
                     /gene_synonym="MEM3; PARK17"
                     /inference="alignment:Splign:2.1.0"
     exon            1206..1413
                     /gene="VPS35"
                     /gene_synonym="MEM3; PARK17"
                     /inference="alignment:Splign:2.1.0"
     exon            1414..1569
                     /gene="VPS35"
                     /gene_synonym="MEM3; PARK17"
                     /inference="alignment:Splign:2.1.0"
     exon            1570..1692
                     /gene="VPS35"
                     /gene_synonym="MEM3; PARK17"
                     /inference="alignment:Splign:2.1.0"
     exon            1693..1872
                     /gene="VPS35"
                     /gene_synonym="MEM3; PARK17"
                     /inference="alignment:Splign:2.1.0"
     exon            1873..2112
                     /gene="VPS35"
                     /gene_synonym="MEM3; PARK17"
                     /inference="alignment:Splign:2.1.0"
     exon            2113..2256
                     /gene="VPS35"
                     /gene_synonym="MEM3; PARK17"
                     /inference="alignment:Splign:2.1.0"
     exon            2257..6776
                     /gene="VPS35"
                     /gene_synonym="MEM3; PARK17"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2606..2611
                     /regulatory_class="polyA_signal_sequence"
                     /gene="VPS35"
                     /gene_synonym="MEM3; PARK17"
                     /note="hexamer: AATAAA"
     polyA_site      2627
                     /gene="VPS35"
                     /gene_synonym="MEM3; PARK17"
                     /note="major polyA site"
     regulatory      3212..3217
                     /regulatory_class="polyA_signal_sequence"
                     /gene="VPS35"
                     /gene_synonym="MEM3; PARK17"
                     /note="hexamer: AATAAA"
     polyA_site      3231
                     /gene="VPS35"
                     /gene_synonym="MEM3; PARK17"
                     /note="major polyA site"
     regulatory      4894..4899
                     /regulatory_class="polyA_signal_sequence"
                     /gene="VPS35"
                     /gene_synonym="MEM3; PARK17"
                     /note="hexamer: AATAAA"
     polyA_site      4929
                     /gene="VPS35"
                     /gene_synonym="MEM3; PARK17"
     regulatory      5773..5778
                     /regulatory_class="polyA_signal_sequence"
                     /gene="VPS35"
                     /gene_synonym="MEM3; PARK17"
                     /note="hexamer: ATTAAA"
     polyA_site      5793
                     /gene="VPS35"
                     /gene_synonym="MEM3; PARK17"
ORIGIN      
        1 acgcgcgggg cgggtgctgc ttgctgcagg ctctggggag tcgccatgcc tacaacacag
       61 cagtcccctc aggatgagca ggaaaagctc ttggatgaag ccatacaggc tgtgaaggtc
      121 cagtcattcc aaatgaagag atgcctggac aaaaacaagc ttatggatgc tctaaaacat
      181 gcttctaata tgcttggtga actccggact tctatgttat caccaaagag ttactatgaa
      241 ctttatatgg ccatttctga tgaactgcac tacttggagg tctacctgac agatgagttt
      301 gctaaaggaa ggaaagtggc agatctctac gaacttgtac agtatgctgg aaacattatc
      361 ccaaggcttt accttttgat cacagttgga gttgtatatg tcaagtcatt tcctcagtcc
      421 aggaaggata ttttgaaaga tttggtagaa atgtgccgtg gtgtgcaaca tcccttgagg
      481 ggtctgtttc ttcgaaatta ccttcttcag tgtaccagaa atatcttacc tgatgaagga
      541 gagccaacag atgaagaaac aactggtgac atcagtgatt ccatggattt tgtactgctc
      601 aactttgcag aaatgaacaa gctctgggtg cgaatgcagc atcagggaca tagccgagat
      661 agagaaaaaa gagaacgaga aagacaagaa ctgagaattt tagtgggaac aaatttggtg
      721 cgcctcagtc agttggaagg tgtaaatgtg gaacgttaca aacagattgt tttgactggc
      781 atattggagc aagttgtaaa ctgtagggat gctttggctc aagaatatct catggagtgt
      841 attattcagg ttttccctga tgaatttcac ctccagactt tgaatccttt tcttcgggcc
      901 tgtgctgagt tacaccagaa tgtaaatgtg aagaacataa tcattgcttt aattgataga
      961 ttagctttat ttgctcaccg tgaagatgga cctggaatcc cagcggatat taaacttttt
     1021 gatatatttt cacagcaggt ggctacagtg atacagtcta gacaagacat gccttcagag
     1081 gatgttgtat ctttacaagt ctctctgatt aatcttgcca tgaaatgtta ccctgatcgt
     1141 gtggactatg ttgataaagt tctagaaaca acagtggaga tattcaataa gctcaacctt
     1201 gaacatattg ctaccagtag tgcagtttca aaggaactca ccagactttt gaaaatacca
     1261 gttgacactt acaacaatat tttaacagtc ttgaaattaa aacattttca cccactcttt
     1321 gagtactttg actacgagtc cagaaagagc atgagttgtt atgtgcttag taatgttctg
     1381 gattataaca cagaaattgt ctctcaagac caggtggatt ccataatgaa tttggtatcc
     1441 acgttgattc aagatcagcc agatcaacct gtagaagacc ctgatccaga agattttgct
     1501 gatgagcaga gccttgtggg ccgcttcatt catctgctgc gctctgagga ccctgaccag
     1561 cagtacttga ttttgaacac agcacgaaaa cattttggag ctggtggaaa tcagcggatt
     1621 cgcttcacac tgccaccttt ggtatttgca gcttaccagc tggcttttcg atataaagag
     1681 aattctaaag tggatgacaa atgggaaaag aaatgccaga agattttttc atttgcccac
     1741 cagactatca gtgctttgat caaagcagag ctggcagaat tgcccttaag actttttctt
     1801 caaggagcac tagctgctgg ggaaattggt tttgaaaatc atgagacagt cgcatatgaa
     1861 ttcatgtccc aggcattttc tctgtatgaa gatgaaatca gcgattccaa agcacagcta
     1921 gctgccatca ccttgatcat tggcactttt gaaaggatga agtgcttcag tgaagagaat
     1981 cacgaacctc tgaggactca gtgtgccctt gctgcatcca aacttctaaa gaaacctgat
     2041 cagggccgag ctgtgagcac ctgtgcacat ctcttctggt ctggcagaaa cacggacaaa
     2101 aatggggagg agcttcacgg aggcaagagg gtaatggagt gcctaaaaaa agctctaaaa
     2161 atagcaaatc agtgcatgga cccctctcta caagtgcagc tttttataga aattctgaac
     2221 agatatatct atttttatga aaaggaaaat gatgcggtaa caattcaggt tttaaaccag
     2281 cttatccaaa agattcgaga agacctcccg aatcttgaat ccagtgaaga aacagagcag
     2341 attaacaaac attttcataa cacactggag catttgcgct tgcggcggga atcaccagaa
     2401 tccgaggggc caatttatga aggtctcatc ctttaaaaag gaaatagctc accatactcc
     2461 tttccatgta catccagtga gggttttatt acgctaggtt tcccttccat agattgtgcc
     2521 tttcagaaat gctgaggtag gtttcccatt tcttacctgt gatgtgtttt acccagcacc
     2581 tccggacact caccttcagg accttaataa aattattcac ttggtaagtg ttcaagtctt
     2641 tctgatcacc ccaagtagca tgactgatct gcaatttaaa attcctgtga tctgtaaaaa
     2701 aaaaaaaaaa aaaaaacaca aaacccacaa gcacttatct tggctactaa tgaagctctc
     2761 cttttttttg tttgtttgtt tgcttcattg ttgattgtgt attttcttca ttcctgggga
     2821 gtactaaccc aaaagcgtct gtctcttgtt ttctagtcca gtttgagatt aatttagaag
     2881 aaaggaatac tgtatgtgaa attcatcttg ggctttcccc taaattgcaa gataaggcca
     2941 tgtgtaagat tttccctaaa actagaatat attaatgcat gtttgagaat tttaaagcac
     3001 catggtcaaa accagaagct atattttgca tatttggact cagccatcca ttaagaaccc
     3061 atgttgtcct ctggacatat ttatcaatat aattgggttt taaatagtat aaaagaaaac
     3121 ttgtgatcta tataatttat gtatcacctt cattgtaaat ttagcaggaa atgcatcaca
     3181 attatgattt ttttttttgc accagtgaaa caataaagat gctattaaca attttggagc
     3241 tttttttccc ctttcatact atctttaatt gataaaaagt actagtagta tggctataga
     3301 gtttaatagt cattctcata tattcacaga gctcacttaa ctctagtttt aagcacttgt
     3361 tgaacaaggc agcatctact cttttagtca tttatacccc ttttctttcc tcaactttac
     3421 cctgcttttc ccaaggcatg atgaattcac ataaaccccc caaaggctca ccaccgatac
     3481 tagttaccta ctatataata aaccatccca aaacttactg tcttaaaaca gcaaacattt
     3541 atctcacagg tctctggatc aggaatttgg gagcagctta gctggtggtt ctggcaaaga
     3601 gtgtggtgta aggttgcaaa caagatactg gttggggctg cagtcatctg atgctttgac
     3661 tggggctgac ttttctgctc actgacctgg ctgttaggca gaggccccag ttcctcttct
     3721 tgagggcaga ggccccagtt cctctccttg agggcagagg cctaagttcc tttccttgag
     3781 ggcagaggcc ccagttcctc tccttgacgg cccttccata gagtgtcctc acagcatggt
     3841 ggctggcttt cccctgagta tgaaagcaag aaggaaacca cagtgccttt cagtggcctc
     3901 cctgtggaag tgacatgctc atttttgcct tattctgtaa gtggggagtc actaagtcta
     3961 gcctatattc aagggtaagg agaattaagc tccacctctt aaagggaaaa tttatagaca
     4021 ttttcaaatg actacatcac ttaacccctc accatctgcc ctcccattgc tagcacttga
     4081 tgactagccc ttgctgggct ttacatgaac agatgtttcc caaagttata aaattagtac
     4141 cactaaaatg tatcaaatgt taagccattc tgtggtatgt catagtccag caaacccttt
     4201 ctgttctttg taggtgttag tttaccccgt ataaacctga ttatggcttg acatgtccat
     4261 attatttaca tgttttgtga aatacaactg gaatagtaga aaagacatga gtcaagaaca
     4321 cttgagttgg agagcttggt ttgcaagtaa ggtaatgtca gtacagtgtg taaattatct
     4381 cttattttct gggttgaggt aaacccaatt ttaaaaataa taaaccaaca aaagtaaata
     4441 aaagtgaaac aaaagcactg ctagtatcct taacttacag attttttttg ttgaacaaaa
     4501 actgggtacc agatatagtt aggcactgaa tctaaaggtg aagaagatca tagcctctag
     4561 cttcaagaac gttagtctgc tgtgaagaca gacctgtagt tacagaagat ggtaagggtt
     4621 tgtgcaggag agcagtgaag aagagctttg agggattggg gatggggaag ttctgcagag
     4681 ggttgtatga attctcatgg aacatcaagt ccaatagttt atttagcctt ctgaggccag
     4741 tgctttggcc tgcagtccca ggctcctcag acccatgggc tgaagcaagt tatactcttg
     4801 gctgcctgta gtgaaataga tatttagaaa ttgacttagc atttaaaacc ctttatagca
     4861 atttgacaga gtaattttat gtctattgga tgtaataaat atttggcttt gtgctttgtg
     4921 tgtctgctat tccctgttct ccttttttag taatttttat tgtgtcttac aaaaaagtac
     4981 ctgtccttaa ttggggtgtg gtggggaatc cctcacaggt ggatggaaac gctgaaccag
     5041 ggcaggcttg gtgctgaatg gcattaaatt ccaagactag gggatagttt ggggggacac
     5101 atctaggaag atgctgtgac tggatgagag atgcctgccg tgttagactt tcctaccctc
     5161 cctttcctgc ctctacccag gaaaccagtg attcttagag tccagatgat cccttctttg
     5221 cctagaaatt catgtttctg actcatcctc aagcctgaaa ttggtggtca tcaggttaat
     5281 gaactgcaaa aacagcccct gctgatagac actgctcaaa gagagaggcg tttgagttga
     5341 tcaggcacca gagcacctag aaggagaatg atgagcactt gtgtacagtt cgtgtgcagc
     5401 tctcaaaggc attgagaaat gtgacagacc tcaaggaata aatgcaccac cacaggcact
     5461 gaggggattt aaacacaaag tctaggttcc agaggggtct tctgttaaca tttgctctct
     5521 cattcagaga cctcaaggta ataagctcaa gcagaaagga tttcccaaca gaattgtctt
     5581 tgacaatggg aattccagac tgcatattgt aaatgaaagg tagagaaatc tgtgcaggag
     5641 accttcctgt ttgctggggt ttgtcctatg gggatgctgt aggcatggac aacagacact
     5701 gattcaggtg ccagtatcta cacatttacc atacacttga attcccaaga gtagtgactt
     5761 gccttaggtt tgattaaaga caaatgtttc ctggcttgtg gtcttgtatt gctgattact
     5821 ccagtagggc accctgcatt gtccaacaaa atggtccatg cttccaaaat ctctggagag
     5881 ggactgtata ttttattttt ttgagacgga gtcttgctct gtcacccaag caggagtgca
     5941 gtggtgcgat cttggctcac tgcaacctcc gcctccccag ttcaagcaat tctcctgcct
     6001 cagcctcccg agtagctggg attacaggcg tgcaccacca tgcctggcta atttttgtat
     6061 ttttagtaca gatgggggtt tcaccatgtt ggccaggctg gtcacgaact tctgaccttg
     6121 tgatccacct ggctcagcct cccaaagtgc tggaattaca ggcatgagcc accttgccca
     6181 gccagattgt atattgtacc actcattgct gtgttcaggc ttgcatcagt agtgtcttca
     6241 aataagtaaa atcttaggct gtgacttaaa tccattttca cttagagaaa tagaaacaca
     6301 aggaaacctt tggacgcttc ataactgctg ggaaaggggt attatcaatt gtgtttgaga
     6361 gtcaaactat aaattacttc ccaaggttag ttctacctat gcccaggaat gaacaaggac
     6421 agcttaaagg ttagaagcaa gatggagtca tttgggtctg atctctttca ctgtcataat
     6481 ttcctcagtt acaatttttg taaaggtggt ttcaaatgct ttgctgacct cccataaaca
     6541 aggatgtgcc aattgtaact tcagttctgc aattcaagtc tgctcccaaa actaaagtcc
     6601 attgaattgc atactgattg ataatgttca ttacgagttt atcttcccat gtgcagagca
     6661 aagacaagag gagatcagcc attcctccac ctacccagag acgttgcatt ggaaaggagc
     6721 cgtatcttct agtatggttt tgcaaatgta aaatgtaaaa tctatgcacg ctcaca
//
