LOCUS       NM_022916               4559 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens VPS33A core subunit of CORVET and HOPS complexes
            (VPS33A), transcript variant 1, mRNA.
ACCESSION   NM_022916
VERSION     NM_022916.6
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4559)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   2  (bases 1 to 4559)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   3  (bases 1 to 4559)
  AUTHORS   Miao,G., Zhao,H., Li,Y., Ji,M., Chen,Y., Shi,Y., Bi,Y., Wang,P. and
            Zhang,H.
  TITLE     ORF3a of the COVID-19 virus SARS-CoV-2 blocks HOPS complex-mediated
            assembly of the SNARE complex required for autolysosome formation
  JOURNAL   Dev Cell 56 (4), 427-442 (2021)
   PUBMED   33422265
REFERENCE   4  (bases 1 to 4559)
  AUTHORS   Dubuke,M.L. and Munson,M.
  TITLE     The Secret Life of Tethers: The Role of Tethering Factors in SNARE
            Complex Regulation
  JOURNAL   Front Cell Dev Biol 4, 42 (2016)
   PUBMED   27243006
  REMARK    Review article
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 4559)
  AUTHORS   Spang,A.
  TITLE     Membrane Tethering Complexes in the Endosomal System
  JOURNAL   Front Cell Dev Biol 4, 35 (2016)
   PUBMED   27243003
  REMARK    Review article
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 4559)
  AUTHORS   Solinger,J.A. and Spang,A.
  TITLE     Tethering complexes in the endocytic pathway: CORVET and HOPS
  JOURNAL   FEBS J 280 (12), 2743-2757 (2013)
   PUBMED   23351085
  REMARK    Review article
REFERENCE   7  (bases 1 to 4559)
  AUTHORS   Gissen,P., Johnson,C.A., Gentle,D., Hurst,L.D., Doherty,A.J.,
            O'Kane,C.J., Kelly,D.A. and Maher,E.R.
  TITLE     Comparative evolutionary analysis of VPS33 homologues: genetic and
            functional insights
  JOURNAL   Hum Mol Genet 14 (10), 1261-1270 (2005)
   PUBMED   15790593
  REMARK    GeneRIF: A and B classes reflect the evolution of
            organelle/tissue-specific functions
REFERENCE   8  (bases 1 to 4559)
  AUTHORS   Suzuki,T., Oiso,N., Gautam,R., Novak,E.K., Panthier,J.J.,
            Suprabha,P.G., Vida,T., Swank,R.T. and Spritz,R.A.
  TITLE     The mouse organellar biogenesis mutant buff results from a mutation
            in Vps33a, a homologue of yeast vps33 and Drosophila carnation
  JOURNAL   Proc Natl Acad Sci U S A 100 (3), 1146-1150 (2003)
   PUBMED   12538872
  REMARK    GeneRIF: VPS33A is mutated in Hermansky-Pudlak syndrome and may
            have a role in melanogenesis
REFERENCE   9  (bases 1 to 4559)
  AUTHORS   Kim,B.Y., Kramer,H., Yamamoto,A., Kominami,E., Kohsaka,S. and
            Akazawa,C.
  TITLE     Molecular characterization of mammalian homologues of class C Vps
            proteins that interact with syntaxin-7
  JOURNAL   J Biol Chem 276 (31), 29393-29402 (2001)
   PUBMED   11382755
REFERENCE   10 (bases 1 to 4559)
  AUTHORS   Huizing,M., Didier,A., Walenta,J., Anikster,Y., Gahl,W.A. and
            Kramer,H.
  TITLE     Molecular cloning and characterization of human VPS18, VPS 11,
            VPS16, and VPS33
  JOURNAL   Gene 264 (2), 241-247 (2001)
   PUBMED   11250079
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BP199163.1, BC016617.1,
            AC048338.29 and AI830457.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On May 17, 2019 this sequence version replaced NM_022916.5.
            
            Summary: This gene encodes a tethering protein and a core subunit
            of the homotypic fusion and protein sorting (HOPS) complex. The
            HOPS complex and a second endosomal tethering complex called the
            class C core vacuole/endosome tethering (CORVET) complex, perform
            diverse functions in endocytosis including membrane tethering,
            RabGTPase interaction, activation and proofreading of
            synaptic-soluble N-ethylmaleimide-sensitive factor attachment
            receptor (SNARE) assembly to drive membrane fusion, and
            endosome-to-cytoskeleton attachment. The HOPS complex controls
            endosome maturation as well as endosome traffic to the lysosome.
            This complex is essential for vacuolar fusion and is required for
            adaptor protein complex 3-dependent transport from the golgi to the
            vacuole. The encoded protein belongs to the Sec1/Munc18 (SM) family
            of SNARE-mediated membrane fusion regulators. Naturally occurring
            mutations in this gene are associated with a novel
            mucopolysaccharidosis-like disease. [provided by RefSeq, Apr 2017].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038197.1877881.1,
                                           SRR14038194.2396236.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000267199.9/ ENSP00000267199.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-65                BP199163.1         28-92
            66-2576             BC016617.1         1-2511
            2577-4338           AC048338.29        98986-100747
            4339-4559           AI830457.1         9-229               c
FEATURES             Location/Qualifiers
     source          1..4559
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="12"
                     /map="12q24.31"
     gene            1..4559
                     /gene="VPS33A"
                     /gene_synonym="MPSPS"
                     /note="VPS33A core subunit of CORVET and HOPS complexes"
                     /db_xref="GeneID:65082"
                     /db_xref="HGNC:HGNC:18179"
                     /db_xref="MIM:610034"
     exon            1..188
                     /gene="VPS33A"
                     /gene_synonym="MPSPS"
                     /inference="alignment:Splign:2.1.0"
     CDS             87..1877
                     /gene="VPS33A"
                     /gene_synonym="MPSPS"
                     /note="isoform 1 is encoded by transcript variant 1;
                     vacuolar protein sorting-associated protein 33A; vacuolar
                     protein sorting 33A; vacuolar protein sorting 33 homolog
                     A; VPS33A, CORVET/HOPS core subunit"
                     /codon_start=1
                     /product="vacuolar protein sorting-associated protein 33A
                     isoform 1"
                     /protein_id="NP_075067.2"
                     /db_xref="CCDS:CCDS9231.1"
                     /db_xref="GeneID:65082"
                     /db_xref="HGNC:HGNC:18179"
                     /db_xref="MIM:610034"
                     /translation="MAAHLSYGRVNLNVLREAVRRELREFLDKCAGSKAIVWDEYLTG
                     PFGLIAQYSLLKEHEVEKMFTLKGNRLPAADVKNIIFFVRPRLELMDIIAENVLSEDR
                     RGPTRDFHILFVPRRSLLCEQRLKDLGVLGSFIHREEYSLDLIPFDGDLLSMESEGAF
                     KECYLEGDQTSLYHAAKGLMTLQALYGTIPQIFGKGECARQVANMMIRMKREFTGSQN
                     SIFPVFDNLLLLDRNVDLLTPLATQLTYEGLIDEIYGIQNSYVKLPPEKFAPKKQGDG
                     GKDLPTEAKKLQLNSAEELYAEIRDKNFNAVGSVLSKKAKIISAAFEERHNAKTVGEI
                     KQFVSQLPHMQAARGSLANHTSIAELIKDVTTSEDFFDKLTVEQEFMSGIDTDKVNNY
                     IEDCIAQKHSLIKVLRLVCLQSVCNSGLKQKVLDYYKREILQTYGYEHILTLHNLEKA
                     GLLKPQTGGRNNYPTIRKTLRLWMDDVNEQNPTDISYVYSGYAPLSVRLAQLLSRPGW
                     RSIEEVLRILPGPHFEERQPLPTGLQKKRQPGENRVTLIFFLGGVTFAEIAALRFLSQ
                     LEDGGTEYVIATTKLMNGTSWIEALMEKPF"
     exon            189..254
                     /gene="VPS33A"
                     /gene_synonym="MPSPS"
                     /inference="alignment:Splign:2.1.0"
     exon            255..382
                     /gene="VPS33A"
                     /gene_synonym="MPSPS"
                     /inference="alignment:Splign:2.1.0"
     exon            383..569
                     /gene="VPS33A"
                     /gene_synonym="MPSPS"
                     /inference="alignment:Splign:2.1.0"
     exon            570..686
                     /gene="VPS33A"
                     /gene_synonym="MPSPS"
                     /inference="alignment:Splign:2.1.0"
     exon            687..861
                     /gene="VPS33A"
                     /gene_synonym="MPSPS"
                     /inference="alignment:Splign:2.1.0"
     exon            862..1055
                     /gene="VPS33A"
                     /gene_synonym="MPSPS"
                     /inference="alignment:Splign:2.1.0"
     exon            1056..1182
                     /gene="VPS33A"
                     /gene_synonym="MPSPS"
                     /inference="alignment:Splign:2.1.0"
     exon            1183..1250
                     /gene="VPS33A"
                     /gene_synonym="MPSPS"
                     /inference="alignment:Splign:2.1.0"
     exon            1251..1388
                     /gene="VPS33A"
                     /gene_synonym="MPSPS"
                     /inference="alignment:Splign:2.1.0"
     exon            1389..1526
                     /gene="VPS33A"
                     /gene_synonym="MPSPS"
                     /inference="alignment:Splign:2.1.0"
     exon            1527..1695
                     /gene="VPS33A"
                     /gene_synonym="MPSPS"
                     /inference="alignment:Splign:2.1.0"
     exon            1696..4559
                     /gene="VPS33A"
                     /gene_synonym="MPSPS"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1981..1986
                     /regulatory_class="polyA_signal_sequence"
                     /gene="VPS33A"
                     /gene_synonym="MPSPS"
                     /note="hexamer: AATATA"
     polyA_site      2028
                     /gene="VPS33A"
                     /gene_synonym="MPSPS"
     regulatory      2550..2555
                     /regulatory_class="polyA_signal_sequence"
                     /gene="VPS33A"
                     /gene_synonym="MPSPS"
                     /note="hexamer: AATAAA"
     polyA_site      2576
                     /gene="VPS33A"
                     /gene_synonym="MPSPS"
                     /note="major polyA site"
     regulatory      4538..4543
                     /regulatory_class="polyA_signal_sequence"
                     /gene="VPS33A"
                     /gene_synonym="MPSPS"
                     /note="hexamer: AATAAA"
     polyA_site      4559
                     /gene="VPS33A"
                     /gene_synonym="MPSPS"
                     /note="major polyA site"
ORIGIN      
        1 gtttggtcac gtgactgcgt ccgtggtcct cccgtaggaa ccggcggact cggttggcgt
       61 tgtggggcag ggggtggtgg agcaagatgg cggctcatct gtcctacggc cgagtgaacc
      121 taaacgtgtt gcgcgaggcg gtgcgtcgcg agctgcgcga gttcctggac aagtgcgcag
      181 gaagcaaggc aatagtttgg gatgaatacc taactggacc ctttggcctg attgcacagt
      241 attcactatt gaaggaacat gaagtggaaa aaatgttcac acttaaagga aatcgtttgc
      301 cggcagctga tgtgaagaat ataatttttt ttgtcagacc caggctagag ttgatggata
      361 taatcgctga aaacgtgctc agtgaagata gacgaggccc aacgagagat tttcatattc
      421 tgtttgtgcc acgccgtagc ctgttgtgcg aacagcggtt gaaggatctg ggtgtcttgg
      481 gatcctttat tcacagggag gagtacagct tagatctcat tccattcgat ggggatctct
      541 tatccatgga atcagagggt gcattcaaag agtgctacct ggagggtgac cagacgagcc
      601 tgtaccacgc agccaagggg ctgatgaccc tgcaagctct gtatggaacg atcccccaga
      661 tctttgggaa aggagaatgc gctcggcaag tggccaatat gatgatcagg atgaagagag
      721 agtttacagg aagccagaat tcaatatttc ctgtttttga taatctcttg ttgcttgatc
      781 ggaatgtgga tttattaaca cctcttgcca ctcagctgac atatgaagga ctcattgatg
      841 aaatttatgg cattcagaac agttatgtga aattacctcc agagaaattt gcacctaaga
      901 aacagggcga tggtggtaag gacctcccca cggaagcaaa gaagctgcag ctgaattctg
      961 cagaggagct ctatgctgag atccgagata agaacttcaa cgcagttggc tctgtgctca
     1021 gcaagaaagc aaagatcatc tctgcagcat tcgaggaaag acacaatgct aagaccgtgg
     1081 gggagatcaa gcagtttgtt tcccagttgc cccacatgca ggcagcaagg ggctcgcttg
     1141 caaaccatac ctcaattgca gaattgatca aagatgtcac tacttctgaa gacttttttg
     1201 ataaattaac cgtggaacag gagtttatgt ctggaataga cactgataag gtcaacaatt
     1261 acattgagga ttgtatcgcc caaaagcact cgttgatcaa ggtgttaaga ctagtttgcc
     1321 tccaatccgt gtgtaatagt gggctcaaac aaaaagtttt ggattattac aaaagagaga
     1381 ttctccagac atacggctat gagcacatat tgaccttaca caacctggag aaggccggcc
     1441 tgctgaaacc gcagacgggg ggcagaaaca attacccaac tatacggaaa acattacgcc
     1501 tctggatgga tgatgttaat gagcaaaacc ccacggacat atcgtatgtg tacagtgggt
     1561 atgccccgct cagtgtgcgg ctggcccagc tgctttcccg gcctggctgg cggagcatcg
     1621 aggaggtcct ccgcatcctc ccagggcccc actttgagga gcggcagcca ctgcccacag
     1681 gactgcagaa gaaacgtcaa ccgggagaaa accgagtgac tctgatattt ttccttgggg
     1741 gcgtaacctt cgctgaaatt gctgccctgc gatttctctc ccagttggaa gatggaggta
     1801 cagaatatgt cattgccacc actaaactaa tgaatggaac cagttggata gaggctctga
     1861 tggaaaaacc tttctaggat gttcagagga gacttaacaa gtgtactgca gaataaacta
     1921 cctctttgaa gaaattgctg aaaggaagta aaaccccata gaagaaacat gggaatacag
     1981 aatatattct ggggtcagct tcttaaatta tactactgtt tactgctttc tccgtctctt
     2041 ttgtattccc tttttttttt tcctttgaga cggagtcttg ctctgtcacc cagactagag
     2101 tgcagtggca cggtctcacc tcactgcaac ctccacctcc taggttcaag caattctcct
     2161 gcctcagcct cctgagtagc tgggactaca ggcatgcacc accacacccg gctaattttt
     2221 gtatttttag tagccatggt gtttcaccat gttggccagg ctggtctcaa actcctgacc
     2281 tcaggtgatc cacctgcctc ggcctcccac agtgctggga ttacaggcct gagccaccgt
     2341 gcctggcccc taatctgctg aagaaagaag aatagaagaa aatcaacctg agtaaaagca
     2401 gcactggttt tgagttttct aagctcaggg tcttcattag agacctctgg aaatacatta
     2461 aggatggtgg gggtagataa tccattcagc cagacaaacg gggccagctc ttaaaataag
     2521 aaagctgaga ctgaggaggt gaaactgaaa ataaaaagag aaagttcatc ctctaatccc
     2581 cagtgctgta tttcttcaag gcgcatcagg cctaggacag tggttaagtg ttgggagctg
     2641 gagatcattt tatcactgag ttgagatttc tttttcctgc agagtgtggg agattatatt
     2701 tagggtcatc agtcactgtc gcaactgatc actaaggcaa gtttgcttcc ttctacttct
     2761 tacaaagcaa gaggtttagg ggagcttctt gcccagaaac aaagtttatg ctctatgtga
     2821 tcagctacat ctccttaggt tccatactta tcaggggctt ttgacatgga agaatgtgat
     2881 aattggggag caaccttctg agaattcagc ctttgaagta attcaaagac agtttcttag
     2941 cacacacgtt aactccagac acagcagtgg tgggtgcctc cattgaggtt tccgttttct
     3001 cttctgtcca tcaagggccc aacatccaac tggtcccaca gtgagcaggg gcaactcttg
     3061 cttcttcagc atctggccac ttcgtttcca tggtaactgc cacgtgtact tttctagcag
     3121 tctgtggcca ctccgtactc agctgaaaac actgtttcag ccccctctct ggtgacctca
     3181 gccttctcca ggtgtatctc ttgatgatct tggagaccag cagccacagc tgctgctact
     3241 cctgcaggag actgtcaggc tgtggtgggg ggcaggggtg ttggaggaga agttgaaaat
     3301 ccgtgtgttc tctgcccctc tgctcctcca tcttagcttc tggaggagtt aaggcaccaa
     3361 gggcaccaag tcaggttggc agttttgctg cccttgccca agcctccagc aaaactagct
     3421 ggtcccctgc tttgctgggt cccagccagg tgtgattttg gttgttgatt agtaaccaca
     3481 cattgttttt ccccaccttt ttcattagtg gaggtttttg ttttgttttg tttttgtttg
     3541 tttgagacag agtctcacgc tcttttcaga ctggagtgca gtggtgcgat gtcagctcac
     3601 tgcaacctcc acttcccggg ttcagacgat tctcatgcct cagcctcctg agtagctggg
     3661 attacagatg tgcaccacca cacctggcta atttttgtat ttttagtaga gatggggttt
     3721 caccatattg gccaggctgg tctcaaactc ctgacctcag gcgattggcc cacctcggcc
     3781 tcccaaagtg ctgggattac aggtgtgaac caccgtgccc ggcccattag tggagttctg
     3841 atgggcacgg taaatattat taatgaaaga gaaattctgt atactttttt aaaaatctct
     3901 ttaatgaaag aatgaagagt ggcagagata ataattgaaa gattagtttc tcatggaatt
     3961 aatttggcct tgatattcag tatttggaaa tgtcttaaag aaaaccattt tcattgtaga
     4021 aaatcctttt ttaaatactt gttatatccc agggagatta gtttatacct tccacctcag
     4081 gcagtcatca gctgctttag agaatggaat ccaccctgcc cctccatttt ttggttcctg
     4141 gggctcaatt caagtctggt caagtctccc tggctgggga ttctgtcctg ggaacagata
     4201 ctcagttgta gtctcatgtg tctgcactca cccaagactc tatatacttg cttgctcaga
     4261 gcatagttag ggcagctcct aaaaatgcat tcagcagaag actgaaaaac ttcaattgct
     4321 tcatttgtat gcaaatagaa aatggtagat aattatatat tatacgcctt tgtgtaagtt
     4381 catttaatac ttgaagcagt ctttgtctca aaagagtatg cccttactga gaaaggatat
     4441 gtgaacggta catttattgt tggggagagt ggattgttta catcatgtct ggacttttgt
     4501 ctagttgggt agacagttct acaccagcag cccccttaat aaagaagtct acacagaca
//
