LOCUS       NP_075067                596 aa            linear   PRI 12-JUN-2025
DEFINITION  vacuolar protein sorting-associated protein 33A isoform 1 [Homo
            sapiens].
ACCESSION   NP_075067
VERSION     NP_075067.2
DBSOURCE    REFSEQ: accession NM_022916.6
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (residues 1 to 596)
  AUTHORS   Zhang,S., Tong,M., Zheng,D., Huang,H., Li,L., Ungermann,C., Pan,Y.,
            Luo,H., Lei,M., Tang,Z., Fu,W., Chen,S., Liu,X. and Zhong,Q.
  TITLE     C9orf72-catalyzed GTP loading of Rab39A enables HOPS-mediated
            membrane tethering and fusion in mammalian autophagy
  JOURNAL   Nat Commun 14 (1), 6360 (2023)
   PUBMED   37821429
  REMARK    Publication Status: Online-Only
REFERENCE   2  (residues 1 to 596)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   3  (residues 1 to 596)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   4  (residues 1 to 596)
  AUTHORS   Dubuke,M.L. and Munson,M.
  TITLE     The Secret Life of Tethers: The Role of Tethering Factors in SNARE
            Complex Regulation
  JOURNAL   Front Cell Dev Biol 4, 42 (2016)
   PUBMED   27243006
  REMARK    Review article
            Publication Status: Online-Only
REFERENCE   5  (residues 1 to 596)
  AUTHORS   Spang,A.
  TITLE     Membrane Tethering Complexes in the Endosomal System
  JOURNAL   Front Cell Dev Biol 4, 35 (2016)
   PUBMED   27243003
  REMARK    Review article
            Publication Status: Online-Only
REFERENCE   6  (residues 1 to 596)
  AUTHORS   Solinger,J.A. and Spang,A.
  TITLE     Tethering complexes in the endocytic pathway: CORVET and HOPS
  JOURNAL   FEBS J 280 (12), 2743-2757 (2013)
   PUBMED   23351085
  REMARK    Review article
REFERENCE   7  (residues 1 to 596)
  AUTHORS   Gissen,P., Johnson,C.A., Gentle,D., Hurst,L.D., Doherty,A.J.,
            O'Kane,C.J., Kelly,D.A. and Maher,E.R.
  TITLE     Comparative evolutionary analysis of VPS33 homologues: genetic and
            functional insights
  JOURNAL   Hum Mol Genet 14 (10), 1261-1270 (2005)
   PUBMED   15790593
  REMARK    GeneRIF: A and B classes reflect the evolution of
            organelle/tissue-specific functions
REFERENCE   8  (residues 1 to 596)
  AUTHORS   Suzuki,T., Oiso,N., Gautam,R., Novak,E.K., Panthier,J.J.,
            Suprabha,P.G., Vida,T., Swank,R.T. and Spritz,R.A.
  TITLE     The mouse organellar biogenesis mutant buff results from a mutation
            in Vps33a, a homologue of yeast vps33 and Drosophila carnation
  JOURNAL   Proc Natl Acad Sci U S A 100 (3), 1146-1150 (2003)
   PUBMED   12538872
  REMARK    GeneRIF: VPS33A is mutated in Hermansky-Pudlak syndrome and may
            have a role in melanogenesis
REFERENCE   9  (residues 1 to 596)
  AUTHORS   Kim,B.Y., Kramer,H., Yamamoto,A., Kominami,E., Kohsaka,S. and
            Akazawa,C.
  TITLE     Molecular characterization of mammalian homologues of class C Vps
            proteins that interact with syntaxin-7
  JOURNAL   J Biol Chem 276 (31), 29393-29402 (2001)
   PUBMED   11382755
REFERENCE   10 (residues 1 to 596)
  AUTHORS   Huizing,M., Didier,A., Walenta,J., Anikster,Y., Gahl,W.A. and
            Kramer,H.
  TITLE     Molecular cloning and characterization of human VPS18, VPS 11,
            VPS16, and VPS33
  JOURNAL   Gene 264 (2), 241-247 (2001)
   PUBMED   11250079
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BP199163.1, BC016617.1,
            AC048338.29 and AI830457.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Jan 10, 2002 this sequence version replaced NP_075067.1.
            
            Summary: This gene encodes a tethering protein and a core subunit
            of the homotypic fusion and protein sorting (HOPS) complex. The
            HOPS complex and a second endosomal tethering complex called the
            class C core vacuole/endosome tethering (CORVET) complex, perform
            diverse functions in endocytosis including membrane tethering,
            RabGTPase interaction, activation and proofreading of
            synaptic-soluble N-ethylmaleimide-sensitive factor attachment
            receptor (SNARE) assembly to drive membrane fusion, and
            endosome-to-cytoskeleton attachment. The HOPS complex controls
            endosome maturation as well as endosome traffic to the lysosome.
            This complex is essential for vacuolar fusion and is required for
            adaptor protein complex 3-dependent transport from the golgi to the
            vacuole. The encoded protein belongs to the Sec1/Munc18 (SM) family
            of SNARE-mediated membrane fusion regulators. Naturally occurring
            mutations in this gene are associated with a novel
            mucopolysaccharidosis-like disease. [provided by RefSeq, Apr 2017].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038197.1877881.1,
                                           SRR14038194.2396236.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000267199.9/ ENSP00000267199.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..596
                     /organism="Homo sapiens"
                     /db_xref="taxon:9606"
                     /chromosome="12"
                     /map="12q24.31"
     Protein         1..596
                     /product="vacuolar protein sorting-associated protein 33A
                     isoform 1"
                     /note="vacuolar protein sorting-associated protein 33A;
                     vacuolar protein sorting 33A; vacuolar protein sorting 33
                     homolog A; VPS33A, CORVET/HOPS core subunit"
                     /calculated_mol_wt=67480
     Region          34..589
                     /region_name="Sec1"
                     /note="Sec1 family; pfam00995"
                     /db_xref="CDD:460020"
     CDS             1..596
                     /gene="VPS33A"
                     /gene_synonym="MPSPS"
                     /coded_by="NM_022916.6:87..1877"
                     /note="isoform 1 is encoded by transcript variant 1"
                     /db_xref="CCDS:CCDS9231.1"
                     /db_xref="GeneID:65082"
                     /db_xref="HGNC:HGNC:18179"
                     /db_xref="MIM:610034"
ORIGIN      
        1 maahlsygrv nlnvlreavr relrefldkc agskaivwde yltgpfglia qysllkehev
       61 ekmftlkgnr lpaadvknii ffvrprlelm diiaenvlse drrgptrdfh ilfvprrsll
      121 ceqrlkdlgv lgsfihreey sldlipfdgd llsmesegaf kecylegdqt slyhaakglm
      181 tlqalygtip qifgkgecar qvanmmirmk reftgsqnsi fpvfdnllll drnvdlltpl
      241 atqltyegli deiygiqnsy vklppekfap kkqgdggkdl pteakklqln saeelyaeir
      301 dknfnavgsv lskkakiisa afeerhnakt vgeikqfvsq lphmqaargs lanhtsiael
      361 ikdvttsedf fdkltveqef msgidtdkvn nyiedciaqk hslikvlrlv clqsvcnsgl
      421 kqkvldyykr eilqtygyeh iltlhnleka gllkpqtggr nnyptirktl rlwmddvneq
      481 nptdisyvys gyaplsvrla qllsrpgwrs ieevlrilpg phfeerqplp tglqkkrqpg
      541 enrvtliffl ggvtfaeiaa lrflsqledg gteyviattk lmngtswiea lmekpf
//
