LOCUS       NM_016208                948 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens VPS28 subunit of ESCRT-I (VPS28), transcript variant
            1, mRNA.
ACCESSION   NM_016208
VERSION     NM_016208.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 948)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   2  (bases 1 to 948)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   3  (bases 1 to 948)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   4  (bases 1 to 948)
  AUTHORS   Flower,T.G., Takahashi,Y., Hudait,A., Rose,K., Tjahjono,N.,
            Pak,A.J., Yokom,A.L., Liang,X., Wang,H.G., Bouamr,F., Voth,G.A. and
            Hurley,J.H.
  TITLE     A helical assembly of human ESCRT-I scaffolds reverse-topology
            membrane scission
  JOURNAL   Nat Struct Mol Biol 27 (6), 570-580 (2020)
   PUBMED   32424346
REFERENCE   5  (bases 1 to 948)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   6  (bases 1 to 948)
  AUTHORS   Tanzi,G.O., Piefer,A.J. and Bates,P.
  TITLE     Equine infectious anemia virus utilizes host vesicular protein
            sorting machinery during particle release
  JOURNAL   J Virol 77 (15), 8440-8447 (2003)
   PUBMED   12857913
  REMARK    GeneRIF: EIAV Gag interacts with a cellular component of the VPS
            machinery, VPS28, to accomplish efficient particle release
REFERENCE   7  (bases 1 to 948)
  AUTHORS   Martin-Serrano,J., Zang,T. and Bieniasz,P.D.
  TITLE     Role of ESCRT-I in retroviral budding
  JOURNAL   J Virol 77 (8), 4794-4804 (2003)
   PUBMED   12663786
REFERENCE   8  (bases 1 to 948)
  AUTHORS   Bishop,N., Horman,A. and Woodman,P.
  TITLE     Mammalian class E vps proteins recognize ubiquitin and act in the
            removal of endosomal protein-ubiquitin conjugates
  JOURNAL   J Cell Biol 157 (1), 91-101 (2002)
   PUBMED   11916981
REFERENCE   9  (bases 1 to 948)
  AUTHORS   Bishop,N. and Woodman,P.
  TITLE     TSG101/mammalian VPS23 and mammalian VPS28 interact directly and
            are recruited to VPS4-induced endosomes
  JOURNAL   J Biol Chem 276 (15), 11735-11742 (2001)
   PUBMED   11134028
REFERENCE   10 (bases 1 to 948)
  AUTHORS   Lippincott-Schwartz,J., Roberts,T.H. and Hirschberg,K.
  TITLE     Secretory protein trafficking and organelle dynamics in living
            cells
  JOURNAL   Annu Rev Cell Dev Biol 16, 557-589 (2000)
   PUBMED   11031247
  REMARK    Review article
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BP380505.1, AK026865.1,
            R72904.1 and BC050438.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_016208.3.
            
            Summary: This gene encodes a protein subunit of the ESCRT-I complex
            (endosomal complexes required for transport), which functions in
            the transport and sorting of proteins into subcellular vesicles.
            This complex can also be hijacked to facilitate the budding of
            enveloped viruses from the cell membrane. Alternative splicing
            results in multiple transcript variants encoding different
            isoforms. [provided by RefSeq, Jul 2013].
            
            Transcript Variant: This variant (1) represents the shorter
            transcript and encodes the shorter isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK026865.1, SRR1163655.365012.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000292510.6/ ENSP00000292510.3
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-35                BP380505.1         19-53
            36-525              AK026865.1         1-490
            526-526             R72904.1           372-372
            527-780             AK026865.1         492-745
            781-948             BC050438.1         1899-2066
FEATURES             Location/Qualifiers
     source          1..948
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="8"
                     /map="8q24.3"
     gene            1..948
                     /gene="VPS28"
                     /gene_synonym="CIIA"
                     /note="VPS28 subunit of ESCRT-I"
                     /db_xref="GeneID:51160"
                     /db_xref="HGNC:HGNC:18178"
                     /db_xref="MIM:611952"
     exon            1..60
                     /gene="VPS28"
                     /gene_synonym="CIIA"
                     /inference="alignment:Splign:2.1.0"
     exon            61..131
                     /gene="VPS28"
                     /gene_synonym="CIIA"
                     /inference="alignment:Splign:2.1.0"
     CDS             95..760
                     /gene="VPS28"
                     /gene_synonym="CIIA"
                     /note="isoform 1 is encoded by transcript variant 1; yeast
                     class E protein Vps28p homolog; vacuolar protein
                     sorting-associated protein 28 homolog; ESCRT-I complex
                     subunit VPS28; vacuolar protein sorting 28-like protein;
                     vacuolar protein sorting 28 homolog; VPS28, ESCRT-I
                     subunit"
                     /codon_start=1
                     /product="vacuolar protein sorting-associated protein 28
                     homolog isoform 1"
                     /protein_id="NP_057292.1"
                     /db_xref="CCDS:CCDS6425.1"
                     /db_xref="GeneID:51160"
                     /db_xref="HGNC:HGNC:18178"
                     /db_xref="MIM:611952"
                     /translation="MFHGIPATPGIGAPGNKPELYEEVKLYKNAREREKYDNMAELFA
                     VVKTMQALEKAYIKDCVSPSEYTAACSRLLVQYKAAFRQVQGSEISSIDEFCRKFRLD
                     CPLAMERIKEDRPITIKDDKGNLNRCIADVVSLFITVMDKLRLEIRAMDEIQPDLREL
                     METMHRMSHLPPDFEGRQTVSQWLQTLSGMSASDELDDSQVRQMLFDLESAYNAFNRF
                     LHA"
     exon            132..160
                     /gene="VPS28"
                     /gene_synonym="CIIA"
                     /inference="alignment:Splign:2.1.0"
     exon            161..198
                     /gene="VPS28"
                     /gene_synonym="CIIA"
                     /inference="alignment:Splign:2.1.0"
     exon            199..288
                     /gene="VPS28"
                     /gene_synonym="CIIA"
                     /inference="alignment:Splign:2.1.0"
     exon            289..394
                     /gene="VPS28"
                     /gene_synonym="CIIA"
                     /inference="alignment:Splign:2.1.0"
     exon            395..496
                     /gene="VPS28"
                     /gene_synonym="CIIA"
                     /inference="alignment:Splign:2.1.0"
     exon            497..550
                     /gene="VPS28"
                     /gene_synonym="CIIA"
                     /inference="alignment:Splign:2.1.0"
     exon            551..642
                     /gene="VPS28"
                     /gene_synonym="CIIA"
                     /inference="alignment:Splign:2.1.0"
     exon            643..948
                     /gene="VPS28"
                     /gene_synonym="CIIA"
                     /inference="alignment:Splign:2.1.0"
     regulatory      926..931
                     /regulatory_class="polyA_signal_sequence"
                     /gene="VPS28"
                     /gene_synonym="CIIA"
                     /note="hexamer: AATAAA"
     polyA_site      948
                     /gene="VPS28"
                     /gene_synonym="CIIA"
                     /note="major polyA site"
ORIGIN      
        1 gagtcgctga ccgagcgcac cccgcccccg gcgccatctt cccgaccgcg agccgtccag
       61 gtctcagtgc tgtgcccccc ccagagccta gaggatgttt catgggatcc cagccacgcc
      121 gggcatagga gcccctggga acaagccgga gctgtatgag gaagtgaagt tgtacaagaa
      181 cgcccgggag agggagaagt acgacaacat ggcagagctg tttgcggtgg tgaagacaat
      241 gcaagccctg gagaaggcct acatcaagga ctgtgtctcc cccagcgagt acactgcagc
      301 ctgctcccgg ctcctggtcc aatacaaagc tgccttcagg caggtccagg gctcagaaat
      361 cagctctatt gacgaattct gccgcaagtt ccgcctggac tgcccgctgg ccatggagcg
      421 gatcaaggag gaccggccca tcaccatcaa ggacgacaag ggcaacctca accgctgcat
      481 cgcagacgtg gtctcgctct tcatcacggt catggacaag ctgcgtctgg agatccgcgc
      541 catggatgag atccagcccg acctgcgaga gctgatggag accatgcacc gcatgagcca
      601 cctcccaccc gactttgagg gccgccagac ggtcagccag tggctgcaga ccctgagcgg
      661 catgtcggcg tcagatgagc tggacgactc acaggtgcgt cagatgctgt tcgacctgga
      721 gtcagcctac aacgccttca accgcttcct gcatgcctga gcccggggca ctagcccttg
      781 cacagaaggg cagagtctga ggcgatggct cctggtcccc tgtccgccac acaggccgtg
      841 gtcatccaca caactcactg tctgcagctg cctgtctggt gtctgtcttt ggtgtcagaa
      901 ctttgggggc cgggcccctc cccacaataa agatgctctc cgaccttc
//
