LOCUS       NM_032353               1088 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens vacuolar protein sorting 25 homolog (VPS25), mRNA.
ACCESSION   NM_032353
VERSION     NM_032353.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   2  (bases 1 to 1088)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   3  (bases 1 to 1088)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   4  (bases 1 to 1088)
  AUTHORS   Fragoza,R., Das,J., Wierbowski,S.D., Liang,J., Tran,T.N., Liang,S.,
            Beltran,J.F., Rivera-Erick,C.A., Ye,K., Wang,T.Y., Yao,L., Mort,M.,
            Stenson,P.D., Cooper,D.N., Wei,X., Keinan,A., Schimenti,J.C.,
            Clark,A.G. and Yu,H.
  TITLE     Extensive disruption of protein interactions by genetic variants
            across the allele frequency spectrum in human populations
  JOURNAL   Nat Commun 10 (1), 4141 (2019)
   PUBMED   31515488
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 1088)
  AUTHORS   Chen,S., Fragoza,R., Klei,L., Liu,Y., Wang,J., Roeder,K., Devlin,B.
            and Yu,H.
  TITLE     An interactome perturbation framework prioritizes damaging missense
            mutations for developmental disorders
  JOURNAL   Nat Genet 50 (7), 1032-1040 (2018)
   PUBMED   29892012
REFERENCE   6  (bases 1 to 1088)
  AUTHORS   Im,Y.J., Wollert,T., Boura,E. and Hurley,J.H.
  TITLE     Structure and function of the ESCRT-II-III interface in
            multivesicular body biogenesis
  JOURNAL   Dev Cell 17 (2), 234-243 (2009)
   PUBMED   19686684
  REMARK    GeneRIF: Results describe the mapping of multivesicular bodies with
            the second winged helix domain of human ESCRT-II subunit VPS25 and
            the first helix of ESCRT-III subunit VPS20.
REFERENCE   7  (bases 1 to 1088)
  AUTHORS   Martin-Serrano,J., Yarovoy,A., Perez-Caballero,D. and Bieniasz,P.D.
  TITLE     Divergent retroviral late-budding domains recruit vacuolar protein
            sorting factors by using alternative adaptor proteins
  JOURNAL   Proc Natl Acad Sci U S A 100 (21), 12414-12419 (2003)
   PUBMED   14519844
  REMARK    Erratum:[Proc Natl Acad Sci U S A. 2003 Dec 9;100(25):152845.
            Yaravoy, A [corrected to Yarovoy, A]]
REFERENCE   8  (bases 1 to 1088)
  AUTHORS   von Schwedler,U.K., Stuchell,M., Muller,B., Ward,D.M., Chung,H.Y.,
            Morita,E., Wang,H.E., Davis,T., He,G.P., Cimbora,D.M., Scott,A.,
            Krausslich,H.G., Kaplan,J., Morham,S.G. and Sundquist,W.I.
  TITLE     The protein network of HIV budding
  JOURNAL   Cell 114 (6), 701-713 (2003)
   PUBMED   14505570
REFERENCE   9  (bases 1 to 1088)
  AUTHORS   Kamura,T., Burian,D., Khalili,H., Schmidt,S.L., Sato,S., Liu,W.J.,
            Conrad,M.N., Conaway,R.C., Conaway,J.W. and Shilatifard,A.
  TITLE     Cloning and characterization of ELL-associated proteins EAP45 and
            EAP20. a role for yeast EAP-like proteins in regulation of gene
            expression by glucose
  JOURNAL   J Biol Chem 276 (19), 16528-16533 (2001)
   PUBMED   11278625
REFERENCE   10 (bases 1 to 1088)
  AUTHORS   Lippincott-Schwartz,J., Roberts,T.H. and Hirschberg,K.
  TITLE     Secretory protein trafficking and organelle dynamics in living
            cells
  JOURNAL   Annu Rev Cell Dev Biol 16, 557-589 (2000)
   PUBMED   11031247
  REMARK    Review article
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BC006282.2.
            
            On Nov 23, 2018 this sequence version replaced NM_032353.3.
            
            Summary: This gene encodes a protein that is a subunit of the
            endosomal sorting complex required for transport II (ESCRT-II).
            This protein complex functions in sorting of ubiquitinated membrane
            proteins during endocytosis. A pseudogene of this gene is present
            on chromosome 1. [provided by RefSeq, Jul 2013].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC006282.2, SRR5189658.41531.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000253794.7/ ENSP00000253794.1
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1088              BC006282.2         14-1101
FEATURES             Location/Qualifiers
     source          1..1088
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="17"
                     /map="17q21.2"
     gene            1..1088
                     /gene="VPS25"
                     /gene_synonym="DERP9; EAP20; FAP20"
                     /note="vacuolar protein sorting 25 homolog"
                     /db_xref="GeneID:84313"
                     /db_xref="HGNC:HGNC:28122"
                     /db_xref="MIM:610907"
     exon            1..80
                     /gene="VPS25"
                     /gene_synonym="DERP9; EAP20; FAP20"
                     /inference="alignment:Splign:2.1.0"
     CDS             28..558
                     /gene="VPS25"
                     /gene_synonym="DERP9; EAP20; FAP20"
                     /note="ESCRT-II complex subunit VPS25; ELL-associated
                     protein of 20 kDa; dermal papilla-derived protein 9"
                     /codon_start=1
                     /product="vacuolar protein-sorting-associated protein 25"
                     /protein_id="NP_115729.1"
                     /db_xref="CCDS:CCDS11438.1"
                     /db_xref="GeneID:84313"
                     /db_xref="HGNC:HGNC:28122"
                     /db_xref="MIM:610907"
                     /translation="MAMSFEWPWQYRFPPFFTLQPNVDTRQKQLAAWCSLVLSFCRLH
                     KQSSMTVMEAQESPLFNNVKLQRKLPVESIQIVLEELRKKGNLEWLDKSKSSFLIMWR
                     RPEEWGKLIYQWVSRSGQNNSVFTLYELTNGEDTEDEEFHGLDEATLLRALQALQQEH
                     KAEIITVSDGRGVKFF"
     exon            81..226
                     /gene="VPS25"
                     /gene_synonym="DERP9; EAP20; FAP20"
                     /inference="alignment:Splign:2.1.0"
     exon            227..280
                     /gene="VPS25"
                     /gene_synonym="DERP9; EAP20; FAP20"
                     /inference="alignment:Splign:2.1.0"
     exon            281..369
                     /gene="VPS25"
                     /gene_synonym="DERP9; EAP20; FAP20"
                     /inference="alignment:Splign:2.1.0"
     exon            370..445
                     /gene="VPS25"
                     /gene_synonym="DERP9; EAP20; FAP20"
                     /inference="alignment:Splign:2.1.0"
     exon            446..1088
                     /gene="VPS25"
                     /gene_synonym="DERP9; EAP20; FAP20"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1070..1075
                     /regulatory_class="polyA_signal_sequence"
                     /gene="VPS25"
                     /gene_synonym="DERP9; EAP20; FAP20"
                     /note="hexamer: ATTAAA"
     polyA_site      1088
                     /gene="VPS25"
                     /gene_synonym="DERP9; EAP20; FAP20"
                     /note="major polyA site"
ORIGIN      
        1 agcttccggg tttcctgggc tactacgatg gcgatgagtt tcgagtggcc gtggcagtat
       61 cgcttcccac ccttctttac gttacaaccg aatgtggaca ctcggcagaa gcagctggcc
      121 gcctggtgct cgctggtcct gtccttctgc cgcctgcaca aacagtccag catgacggtg
      181 atggaagctc aggagagccc gctcttcaac aacgtcaagc tacagcgaaa gcttcctgtg
      241 gagtcgatcc agattgtatt agaggaactg aggaagaaag ggaacctcga gtggttggat
      301 aagagcaagt ccagcttcct gatcatgtgg cggaggccag aagaatgggg gaaactcatc
      361 tatcagtggg tttccaggag tggccagaac aactccgtct ttaccctgta tgaactgact
      421 aatggggaag acacagagga tgaggagttc cacgggctgg atgaagccac tctactgcgg
      481 gctctgcagg ccctacagca ggagcacaag gccgagatca tcactgtcag cgatggccga
      541 ggcgtcaagt tcttctagca gggacctgtc tccctttact tcttacctcc cacctttcca
      601 gggctttcaa aaggagacag acccagtgtc ccccaaagac tggatctgtg actccaccag
      661 actcaaaagg actccagtcc tgaaggctgg gacctgggga tgggtttctc acaccccata
      721 tgtctgtccc ttggataggg tgaggctgaa gcaccaggga gaaaatatgt gcttcttctc
      781 gccctacctc ctttcccatc ctagactgtc cttgagccag ggtctgtaaa cctgacactt
      841 tatatgtgtt cacacatgta agtacataca cacatgcgcc tgcagcacat gcttctgtct
      901 cctcctcctc ccaccccttt agctgctgtt gcctcccttc tcaggctggt gctggatcct
      961 tcctagggga tgggggaagc cctggctgca ggcagccttc caggcaatat gaagatagga
     1021 ggcccacggg cctggcagtg agaggtgtgg ccccacaccg atttatgata ttaaaatctc
     1081 aactccca
//
