LOCUS       NM_020857               3875 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens VPS18 core subunit of CORVET and HOPS complexes
            (VPS18), mRNA.
ACCESSION   NM_020857 NM_080432 XM_378171
VERSION     NM_020857.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3875)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   2  (bases 1 to 3875)
  AUTHORS   Niu,H., Qian,L., Luo,Y., Wang,F., Zheng,H., Gao,Y., Wang,H., Hu,X.,
            Yuan,H. and Lou,H.
  TITLE     Targeting of VPS18 by the lysosomotropic agent RDN reverses
            TFE3-mediated drug resistance
  JOURNAL   Signal Transduct Target Ther 6 (1), 224 (2021)
   PUBMED   34099617
  REMARK    GeneRIF: Targeting of VPS18 by the lysosomotropic agent RDN
            reverses TFE3-mediated drug resistance.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3875)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   4  (bases 1 to 3875)
  AUTHORS   Miao,G., Zhao,H., Li,Y., Ji,M., Chen,Y., Shi,Y., Bi,Y., Wang,P. and
            Zhang,H.
  TITLE     ORF3a of the COVID-19 virus SARS-CoV-2 blocks HOPS complex-mediated
            assembly of the SNARE complex required for autolysosome formation
  JOURNAL   Dev Cell 56 (4), 427-442 (2021)
   PUBMED   33422265
REFERENCE   5  (bases 1 to 3875)
  AUTHORS   van der Beek,J., Jonker,C., van der Welle,R., Liv,N. and
            Klumperman,J.
  TITLE     CORVET, CHEVI and HOPS - multisubunit tethers of the endo-lysosomal
            system in health and disease
  JOURNAL   J Cell Sci 132 (10) (2019)
   PUBMED   31092635
  REMARK    Review article
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 3875)
  AUTHORS   Yogosawa,S., Kawasaki,M., Wakatsuki,S., Kominami,E., Shiba,Y.,
            Nakayama,K., Kohsaka,S. and Akazawa,C.
  TITLE     Monoubiquitylation of GGA3 by hVPS18 regulates its
            ubiquitin-binding ability
  JOURNAL   Biochem Biophys Res Commun 350 (1), 82-90 (2006)
   PUBMED   16996030
  REMARK    GeneRIF: ubiquitin binding and ubiquitylation of GGA3-GAT domain
            are mutually inseparable through a ubiquitin ligase activity of
            hVPS18
REFERENCE   7  (bases 1 to 3875)
  AUTHORS   Yogosawa,S., Hatakeyama,S., Nakayama,K.I., Miyoshi,H., Kohsaka,S.
            and Akazawa,C.
  TITLE     Ubiquitylation and degradation of serum-inducible kinase by hVPS18,
            a RING-H2 type ubiquitin ligase
  JOURNAL   J Biol Chem 280 (50), 41619-41627 (2005)
   PUBMED   16203730
  REMARK    GeneRIF: hVPS18 may play an important role in regulation of SNK
            activity through its ubiquitin ligase
REFERENCE   8  (bases 1 to 3875)
  AUTHORS   Kim,B.Y., Ueda,M., Kominami,E., Akagawa,K., Kohsaka,S. and
            Akazawa,C.
  TITLE     Identification of mouse Vps16 and biochemical characterization of
            mammalian class C Vps complex
  JOURNAL   Biochem Biophys Res Commun 311 (3), 577-582 (2003)
   PUBMED   14623309
REFERENCE   9  (bases 1 to 3875)
  AUTHORS   Kim,B.Y., Kramer,H., Yamamoto,A., Kominami,E., Kohsaka,S. and
            Akazawa,C.
  TITLE     Molecular characterization of mammalian homologues of class C Vps
            proteins that interact with syntaxin-7
  JOURNAL   J Biol Chem 276 (31), 29393-29402 (2001)
   PUBMED   11382755
REFERENCE   10 (bases 1 to 3875)
  AUTHORS   Huizing,M., Didier,A., Walenta,J., Anikster,Y., Gahl,W.A. and
            Kramer,H.
  TITLE     Molecular cloning and characterization of human VPS18, VPS 11,
            VPS16, and VPS33
  JOURNAL   Gene 264 (2), 241-247 (2001)
   PUBMED   11250079
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL713725.1 and AF308802.1.
            
            On Nov 24, 2018 this sequence version replaced NM_020857.2.
            
            Summary: Vesicle mediated protein sorting plays an important role
            in segregation of intracellular molecules into distinct organelles.
            Genetic studies in yeast have identified more than 40 vacuolar
            protein sorting (VPS) genes involved in vesicle transport to
            vacuoles. This gene encodes the human homolog of yeast class C
            Vps18 protein. The mammalian class C Vps proteins are predominantly
            associated with late endosomes/lysosomes, and like their yeast
            counterparts, may mediate vesicle trafficking steps in the
            endosome/lysosome pathway. [provided by RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AL713725.1, AB040908.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000220509.10/ ENSP00000220509.5
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-3259              AL713725.1         21-3279
            3260-3875           AF308802.1         3255-3870
FEATURES             Location/Qualifiers
     source          1..3875
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="15"
                     /map="15q15.1"
     gene            1..3875
                     /gene="VPS18"
                     /gene_synonym="PEP3"
                     /note="VPS18 core subunit of CORVET and HOPS complexes"
                     /db_xref="GeneID:57617"
                     /db_xref="HGNC:HGNC:15972"
                     /db_xref="MIM:608551"
     exon            1..410
                     /gene="VPS18"
                     /gene_synonym="PEP3"
                     /inference="alignment:Splign:2.1.0"
     CDS             320..3241
                     /gene="VPS18"
                     /gene_synonym="PEP3"
                     /note="vacuolar protein sorting protein 18; hVPS18;
                     vacuolar protein sorting 18 homolog; VPS18, CORVET/HOPS
                     core subunit"
                     /codon_start=1
                     /product="vacuolar protein sorting-associated protein 18
                     homolog"
                     /protein_id="NP_065908.1"
                     /db_xref="CCDS:CCDS10069.1"
                     /db_xref="GeneID:57617"
                     /db_xref="HGNC:HGNC:15972"
                     /db_xref="MIM:608551"
                     /translation="MASILDEYENSLSRSAVLQPGCPSVGIPHSGYVNAQLEKEVPIF
                     TKQRIDFTPSERITSLVVSSNQLCMSLGKDTLLRIDLGKANEPNHVELGRKDDAKVHK
                     MFLDHTGSHLLIALSSTEVLYVNRNGQKVRPLARWKGQLVESVGWNKALGTESSTGPI
                     LVGTAQGHIFEAELSASEGGLFGPAPDLYFRPLYVLNEEGGPAPVCSLEAERGPDGRS
                     FVIATTRQRLFQFIGRAAEGAEAQGFSGLFAAYTDHPPPFREFPSNLGYSELAFYTPK
                     LRSAPRAFAWMMGDGVLYGALDCGRPDSLLSEERVWEYPEGVGPGASPPLAIVLTQFH
                     FLLLLADRVEAVCTLTGQVVLRDHFLEKFGPLKHMVKDSSTGQLWAYTERAVFRYHVQ
                     REARDVWRTYLDMNRFDLAKEYCRERPDCLDTVLAREADFCFRQRRYLESARCYALTQ
                     SYFEEIALKFLEARQEEALAEFLQRKLASLKPAERTQATLLTTWLTELYLSRLGALQG
                     DPEALTLYRETKECFRTFLSSPRHKEWLFASRASIHELLASHGDTEHMVYFAVIMQDY
                     ERVVAYHCQHEAYEEALAVLARHRDPQLFYKFSPILIRHIPRQLVDAWIEMGSRLDAR
                     QLIPALVNYSQGGEVQQVSQAIRYMEFCVNVLGETEQAIHNYLLSLYARGRPDSLLAY
                     LEQAGASPHRVHYDLKYALRLCAEHGHHRACVHVYKVLELYEEAVDLALQVDVDLAKQ
                     CADLPEEDEELRKKLWLKIARHVVQEEEDVQTAMACLASCPLLKIEDVLPFFPDFVTI
                     DHFKEAICSSLKAYNHHIQELQREMEEATASAQRIRRDLQELRGRYGTVEPQDKCATC
                     DFPLLNRPFYLFLCGHMFHADCLLQAVRPGLPAYKQARLEELQRKLGAAPPPAKGSAR
                     AKEAEGGAATAGPSREQLKADLDELVAAECVYCGELMIRSIDRPFIDPQRYEEEQLSW
                     L"
     exon            411..552
                     /gene="VPS18"
                     /gene_synonym="PEP3"
                     /inference="alignment:Splign:2.1.0"
     exon            553..644
                     /gene="VPS18"
                     /gene_synonym="PEP3"
                     /inference="alignment:Splign:2.1.0"
     exon            645..2515
                     /gene="VPS18"
                     /gene_synonym="PEP3"
                     /inference="alignment:Splign:2.1.0"
     exon            2516..3875
                     /gene="VPS18"
                     /gene_synonym="PEP3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3856..3861
                     /regulatory_class="polyA_signal_sequence"
                     /gene="VPS18"
                     /gene_synonym="PEP3"
                     /note="hexamer: ATTAAA"
     polyA_site      3875
                     /gene="VPS18"
                     /gene_synonym="PEP3"
                     /note="major polyA site"
ORIGIN      
        1 agtcagctga gctgccgggg cgaggttggg atcacctggc accggctgaa gggagcctgt
       61 gatttttttg tagcgggggc ggggagtaag gtgcaagact gcgccagatt caaggacgag
      121 ggctgcccga ttatctcgct gcataaggca agagcaagag gatcctcagg attttaaaga
      181 ggaggcgacg gctgcaggtt cccaggatct gtcagaggct ggggagttac agcttccatt
      241 ctggggcgac ggggaccccg ggggggtagc ccttttgtaa tccccaggcc ccggacaaag
      301 agcccagagg ccgggcacca tggcgtccat cctggatgag tacgagaact cgctgtcccg
      361 ctcggccgtc ttgcagcccg gctgccctag cgtgggcatc ccccactcgg ggtatgtgaa
      421 tgcccagctg gagaaggaag tgcccatctt cacaaagcag cgcattgact tcaccccttc
      481 cgagcgcatt accagtcttg tcgtctccag caatcagctg tgcatgagcc tgggcaagga
      541 tacactgctc cgcattgact tgggcaaggc aaatgagccc aaccacgtgg agctgggacg
      601 taaggatgac gcaaaagttc acaagatgtt ccttgaccat actggctctc acctgctgat
      661 tgccctgagc agcacggagg tcctctacgt gaaccgaaat ggacagaagg tacggccact
      721 agcacgctgg aaggggcagc tggtggagag tgtgggttgg aacaaggcac tgggcacgga
      781 gagcagcaca ggccccatcc tggtcgggac tgcccaaggc cacatctttg aagcagagct
      841 ctcagccagc gaaggtgggc ttttcggccc tgctccggat ctctacttcc gcccattgta
      901 cgtgctaaat gaagaagggg gtccagcacc tgtgtgctcc cttgaggccg agcggggccc
      961 tgatgggcgt agctttgtta ttgccaccac tcggcagcgc ctcttccagt tcataggccg
     1021 agcagcagag ggggctgagg cccagggttt ctcagggctc tttgcagctt acacggacca
     1081 cccaccccca ttccgtgagt ttcccagcaa cctgggctac agtgagttgg ccttctacac
     1141 ccccaagctg cgctccgcac cccgggcctt cgcctggatg atgggggatg gtgtgttgta
     1201 tggggcattg gactgtgggc gccctgactc tctgctgagc gaggagcgag tctgggagta
     1261 cccagagggg gtagggcctg gggccagccc acccctagcc atcgtcttga cccagttcca
     1321 cttcctgctg ctactggcag accgggtgga ggcagtgtgc acactgaccg ggcaggtggt
     1381 gctgcgggat cacttcctgg agaaatttgg gccgctgaag cacatggtga aggactcctc
     1441 cacaggccag ctgtgggcct acactgagcg ggctgtcttc cgctaccacg tgcaacggga
     1501 ggcccgagat gtctggcgca cctatctgga catgaaccgc ttcgatctgg ccaaagagta
     1561 ttgtcgagag cggcccgact gcctggacac ggtcctggcc cgggaggccg atttctgctt
     1621 tcgccagcgt cgctacctgg agagcgcacg ctgctatgcc ctgacccaga gctactttga
     1681 ggagattgcc ctcaagttcc tggaggcccg acaggaggag gctctggctg agttcctgca
     1741 gcgaaaactg gccagtttga agccagccga acgtacccag gccacactgc tgaccacctg
     1801 gctgacagag ctctacctga gccggcttgg ggctctgcag ggcgacccag aggccctgac
     1861 tctctaccga gaaaccaagg aatgctttcg aaccttcctc agcagccccc gccacaaaga
     1921 gtggctcttt gccagccggg cctctatcca tgagctgctc gccagtcatg gggacacaga
     1981 acacatggtg tactttgcag tgatcatgca ggactatgag cgggtggtgg cttaccactg
     2041 tcagcacgag gcctacgagg aggccctggc cgtgctcgcc cgccaccgtg acccccagct
     2101 cttctacaag ttctcaccca tcctcatccg tcacatcccc cgccagcttg tagatgcctg
     2161 gattgagatg ggcagccggc tggatgctcg tcagctcatt cctgccctgg tgaactacag
     2221 ccagggtggt gaggtccagc aggtgagcca ggccatccgc tacatggagt tctgcgtgaa
     2281 cgtgctgggg gagactgagc aggccatcca caactacctg ctgtcactgt atgcccgtgg
     2341 ccggccggac tcactactgg cctatctgga gcaggctggg gccagccccc accgggtgca
     2401 ttacgacctc aagtatgcgc tgcggctctg cgccgagcat ggccaccacc gcgcttgtgt
     2461 ccatgtctac aaggtcctag agctgtatga ggaggccgtg gacctggccc tgcaggtgga
     2521 tgtggacctg gccaagcagt gtgcagacct gcctgaggag gatgaggaat tgcgcaagaa
     2581 gctgtggctg aagatcgcac ggcacgtggt gcaggaagag gaagatgtac agacagccat
     2641 ggcttgcctg gctagctgcc ccttgctcaa gattgaggat gtgctgccct tctttcctga
     2701 tttcgtcacc atcgaccact tcaaggaggc gatctgcagc tcacttaagg cctacaacca
     2761 ccacatccag gagctgcagc gggagatgga agaggctaca gccagtgccc agcgcatccg
     2821 gcgagacctg caggagctgc ggggccgcta cggcactgtg gagccccagg acaaatgtgc
     2881 cacctgcgac ttccccctgc tcaaccgccc tttttacctc ttcctctgtg gccatatgtt
     2941 ccatgctgac tgcctgctgc aggctgtgcg acctggcctg ccagcctaca agcaggcccg
     3001 gctggaggag ctgcagagga agctgggggc tgctccaccc ccagccaagg gctctgcccg
     3061 ggccaaggag gccgagggtg gggctgccac ggcagggccc agccgggaac agctcaaggc
     3121 tgacctggat gagttggtgg ccgctgagtg tgtgtactgt ggggagctga tgatccgctc
     3181 tatcgaccgg ccgttcatcg acccccagcg ctacgaggag gagcagctca gttggctgta
     3241 ggagggtgtc acctttgatg gggggtgggc aatggggagc agtggcttga acccacttga
     3301 gaaggctgcc tcctaggctc tgctcagtca tcttgcaatt gccacactgt gaccacgttg
     3361 acgggagtag agtagcgctg ttggccagga ggtgtcaggt gtgagtgtat tctgccagct
     3421 tttcatgctg ttcttcagag ctgcagttat gccagaccat cagcctgcct cccagtagag
     3481 gcccttcacc tggagaagtc agaaatctga cccaattcca ccccctgcct ctagcacctc
     3541 ttctgtccct gtcattcccc acacacgtcc tgttcacctc gagagagaga gagagagagc
     3601 acctttcttc cgtctgttca ctctgcggcc tctggaatcc cagctcttct ctctcagaag
     3661 aagccttctc ttcctcctgc ctgtaggtgt cccagaagtg agaaggcagc cttcgaagtc
     3721 ctgggcattg ggtgagaaag tgatgctagt tggggcatgc ttttgtgcac actctctggg
     3781 gctccagtgt gaagggtgcc ctggggctga gggccttgtg gaggatggtc ggtggtggtg
     3841 atggaggtgg agagcattaa actgtctgca ctgca
//
