LOCUS       NM_007127               6500 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens villin 1 (VIL1), mRNA.
ACCESSION   NM_007127
VERSION     NM_007127.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6500)
  AUTHORS   Xu,J., Zou,J., Wu,L. and Lu,W.
  TITLE     Transcriptome analysis uncovers the diagnostic value of
            miR-192-5p/HNF1A-AS1/VIL1 panel in cervical adenocarcinoma
  JOURNAL   Sci Rep 10 (1), 16584 (2020)
   PUBMED   33024199
  REMARK    GeneRIF: Transcriptome analysis uncovers the diagnostic value of
            miR-192-5p/HNF1A-AS1/VIL1 panel in cervical adenocarcinoma.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6500)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   3  (bases 1 to 6500)
  AUTHORS   George,S.P., Esmaeilniakooshkghazi,A., Roy,S. and Khurana,S.
  TITLE     F-actin-bundling sites are conserved in proteins with villin-type
            headpiece domains
  JOURNAL   Mol Biol Cell 31 (17), 1857-1866 (2020)
   PUBMED   32520642
  REMARK    GeneRIF: F-actin-bundling sites are conserved in proteins with
            villin-type headpiece domains.
REFERENCE   4  (bases 1 to 6500)
  AUTHORS   Roy,S., Esmaeilniakooshkghazi,A., Patnaik,S., Wang,Y., George,S.P.,
            Ahrorov,A., Hou,J.K., Herron,A.J., Sesaki,H. and Khurana,S.
  TITLE     Villin-1 and Gelsolin Regulate Changes in Actin Dynamics That
            Affect Cell Survival Signaling Pathways and Intestinal Inflammation
  JOURNAL   Gastroenterology 154 (5), 1405-1420 (2018)
   PUBMED   29274870
  REMARK    GeneRIF: Induction of cell stress alters the actin cytoskeleton in
            intestinal epithelial cells via changes in the actin-binding
            proteins villin-1 and gelsolin.
REFERENCE   5  (bases 1 to 6500)
  AUTHORS   Zhao,L., Huang,S., Liu,J., Zhao,J., Li,Q. and Wang,H.Q.
  TITLE     Clinicopathological, radiographic, and oncogenic features of
            primary pulmonary enteric adenocarcinoma in comparison with
            invasive adenocarcinoma in resection specimens
  JOURNAL   Medicine (Baltimore) 96 (39), e8153 (2017)
   PUBMED   28953659
  REMARK    GeneRIF: Villin has a higher positive expression in Primary
            Pulmonary Enteric Adenocarcinoma cases. Villin proved to be a very
            sensitive and helpful enteric marker in the differential diagnosis
            between PEAC and other lung adenocarcinomas.
REFERENCE   6  (bases 1 to 6500)
  AUTHORS   Pringault,E., Robine,S. and Louvard,D.
  TITLE     Structure of the human villin gene
  JOURNAL   Proc Natl Acad Sci U S A 88 (23), 10811-10815 (1991)
   PUBMED   1961750
REFERENCE   7  (bases 1 to 6500)
  AUTHORS   Arpin,M., Pringault,E., Finidori,J., Garcia,A., Jeltsch,J.M.,
            Vandekerckhove,J. and Louvard,D.
  TITLE     Sequence of human villin: a large duplicated domain homologous with
            other actin-severing proteins and a unique small carboxy-terminal
            domain related to villin specificity
  JOURNAL   J Cell Biol 107 (5), 1759-1766 (1988)
   PUBMED   2846586
REFERENCE   8  (bases 1 to 6500)
  AUTHORS   Rousseau-Merck,M.F., Simon-Chazottes,D., Arpin,M., Pringault,E.,
            Louvard,D., Guenet,J.L. and Berger,R.
  TITLE     Localization of the villin gene on human chromosome 2q35-q36 and on
            mouse chromosome 1
  JOURNAL   Hum Genet 78 (2), 130-133 (1988)
   PUBMED   2892781
REFERENCE   9  (bases 1 to 6500)
  AUTHORS   Pringault,E., Arpin,M., Garcia,A., Finidori,J. and Louvard,D.
  TITLE     A human villin cDNA clone to investigate the differentiation of
            intestinal and kidney cells in vivo and in culture
  JOURNAL   EMBO J 5 (12), 3119-3124 (1986)
   PUBMED   3453110
REFERENCE   10 (bases 1 to 6500)
  AUTHORS   Hesterberg,L.K. and Weber,K.
  TITLE     Isolation of a domain of villin retaining calcium-dependent
            interaction with G-actin, but devoid of F-actin fragmenting
            activity
  JOURNAL   Eur J Biochem 154 (1), 135-140 (1986)
   PUBMED   3510866
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BP329402.1, AK313709.1,
            AK223398.1 and AC073838.6.
            
            On May 17, 2019 this sequence version replaced NM_007127.2.
            
            Summary: This gene encodes a member of a family of
            calcium-regulated actin-binding proteins.  This protein represents
            a dominant part of the brush border cytoskeleton which functions in
            the capping, severing, and bundling of actin filaments.  Two mRNAs
            of 2.7 kb and 3.5 kb have been observed; they result from
            utilization of alternate poly-adenylation signals present in the
            terminal exon. [provided by RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK313709.1, AK223398.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968540, SAMEA1970526
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000248444.10/ ENSP00000248444.5
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-568               BP329402.1         9-576
            569-2541            AK313709.1         569-2541
            2542-2730           AK223398.1         2544-2732
            2731-6500           AC073838.6         3846-7615
FEATURES             Location/Qualifiers
     source          1..6500
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2q35"
     gene            1..6500
                     /gene="VIL1"
                     /gene_synonym="D2S1471; VIL"
                     /note="villin 1"
                     /db_xref="GeneID:7429"
                     /db_xref="HGNC:HGNC:12690"
                     /db_xref="MIM:193040"
     exon            1..46
                     /gene="VIL1"
                     /gene_synonym="D2S1471; VIL"
                     /inference="alignment:Splign:2.1.0"
     exon            47..132
                     /gene="VIL1"
                     /gene_synonym="D2S1471; VIL"
                     /inference="alignment:Splign:2.1.0"
     CDS             58..2541
                     /gene="VIL1"
                     /gene_synonym="D2S1471; VIL"
                     /codon_start=1
                     /product="villin-1"
                     /protein_id="NP_009058.2"
                     /db_xref="CCDS:CCDS2417.1"
                     /db_xref="GeneID:7429"
                     /db_xref="HGNC:HGNC:12690"
                     /db_xref="MIM:193040"
                     /translation="MTKLSAQVKGSLNITTPGLQIWRIEAMQMVPVPSSTFGSFFDGD
                     CYIILAIHKTASSLSYDIHYWIGQDSSLDEQGAAAIYTTQMDDFLKGRAVQHREVQGN
                     ESEAFRGYFKQGLVIRKGGVASGMKHVETNSYDVQRLLHVKGKRNVVAGEVEMSWKSF
                     NRGDVFLLDLGKLIIQWNGPESTRMERLRGMTLAKEIRDQERGGRTYVGVVDGENELA
                     SPKLMEVMNHVLGKRRELKAAVPDTVVEPALKAALKLYHVSDSEGNLVVREVATRPLT
                     QDLLSHEDCYILDQGGLKIYVWKGKKANEQEKKGAMSHALNFIKAKQYPPSTQVEVQN
                     DGAESAVFQQLFQKWTASNRTSGLGKTHTVGSVAKVEQVKFDATSMHVKPQVAAQQKM
                     VDDGSGEVQVWRIENLELVPVDSKWLGHFYGGDCYLLLYTYLIGEKQHYLLYVWQGSQ
                     ASQDEITASAYQAVILDQKYNGEPVQIRVPMGKEPPHLMSIFKGRMVVYQGGTSRTNN
                     LETGPSTRLFQVQGTGANNTKAFEVPARANFLNSNDVFVLKTQSCCYLWCGKGCSGDE
                     REMAKMVADTISRTEKQVVVEGQEPANFWMALGGKAPYANTKRLQEENLVITPRLFEC
                     SNKTGRFLATEIPDFNQDDLEEDDVFLLDVWDQVFFWIGKHANEEEKKAAATTAQEYL
                     KTHPSGRDPETPIIVVKQGHEPPTFTGWFLAWDPFKWSNTKSYEDLKAELGNSRDWSQ
                     ITAEVTSPKVDVFNANSNLSSGPLPIFPLEQLVNKPVEELPEGVDPSRKEEHLSIEDF
                     TQAFGMTPAAFSALPRWKQQNLKKEKGLF"
     exon            133..207
                     /gene="VIL1"
                     /gene_synonym="D2S1471; VIL"
                     /inference="alignment:Splign:2.1.0"
     exon            208..404
                     /gene="VIL1"
                     /gene_synonym="D2S1471; VIL"
                     /inference="alignment:Splign:2.1.0"
     exon            405..513
                     /gene="VIL1"
                     /gene_synonym="D2S1471; VIL"
                     /inference="alignment:Splign:2.1.0"
     exon            514..624
                     /gene="VIL1"
                     /gene_synonym="D2S1471; VIL"
                     /inference="alignment:Splign:2.1.0"
     exon            625..827
                     /gene="VIL1"
                     /gene_synonym="D2S1471; VIL"
                     /inference="alignment:Splign:2.1.0"
     exon            828..906
                     /gene="VIL1"
                     /gene_synonym="D2S1471; VIL"
                     /inference="alignment:Splign:2.1.0"
     exon            907..1005
                     /gene="VIL1"
                     /gene_synonym="D2S1471; VIL"
                     /inference="alignment:Splign:2.1.0"
     exon            1006..1159
                     /gene="VIL1"
                     /gene_synonym="D2S1471; VIL"
                     /inference="alignment:Splign:2.1.0"
     exon            1160..1260
                     /gene="VIL1"
                     /gene_synonym="D2S1471; VIL"
                     /inference="alignment:Splign:2.1.0"
     exon            1261..1398
                     /gene="VIL1"
                     /gene_synonym="D2S1471; VIL"
                     /inference="alignment:Splign:2.1.0"
     exon            1399..1557
                     /gene="VIL1"
                     /gene_synonym="D2S1471; VIL"
                     /inference="alignment:Splign:2.1.0"
     exon            1558..1737
                     /gene="VIL1"
                     /gene_synonym="D2S1471; VIL"
                     /inference="alignment:Splign:2.1.0"
     exon            1738..1883
                     /gene="VIL1"
                     /gene_synonym="D2S1471; VIL"
                     /inference="alignment:Splign:2.1.0"
     exon            1884..2028
                     /gene="VIL1"
                     /gene_synonym="D2S1471; VIL"
                     /inference="alignment:Splign:2.1.0"
     exon            2029..2217
                     /gene="VIL1"
                     /gene_synonym="D2S1471; VIL"
                     /inference="alignment:Splign:2.1.0"
     exon            2218..2286
                     /gene="VIL1"
                     /gene_synonym="D2S1471; VIL"
                     /inference="alignment:Splign:2.1.0"
     exon            2287..2427
                     /gene="VIL1"
                     /gene_synonym="D2S1471; VIL"
                     /inference="alignment:Splign:2.1.0"
     exon            2428..6500
                     /gene="VIL1"
                     /gene_synonym="D2S1471; VIL"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2688..2693
                     /regulatory_class="polyA_signal_sequence"
                     /gene="VIL1"
                     /gene_synonym="D2S1471; VIL"
                     /note="hexamer: AATAAT"
     polyA_site      2727
                     /gene="VIL1"
                     /gene_synonym="D2S1471; VIL"
                     /note="major polyA site"
     regulatory      3463..3468
                     /regulatory_class="polyA_signal_sequence"
                     /gene="VIL1"
                     /gene_synonym="D2S1471; VIL"
                     /note="hexamer: AATAAA"
     polyA_site      3495
                     /gene="VIL1"
                     /gene_synonym="D2S1471; VIL"
     regulatory      4071..4076
                     /regulatory_class="polyA_signal_sequence"
                     /gene="VIL1"
                     /gene_synonym="D2S1471; VIL"
                     /note="hexamer: AATAAA"
     polyA_site      4092
                     /gene="VIL1"
                     /gene_synonym="D2S1471; VIL"
     regulatory      4338..4343
                     /regulatory_class="polyA_signal_sequence"
                     /gene="VIL1"
                     /gene_synonym="D2S1471; VIL"
                     /note="hexamer: AATAAA"
     polyA_site      4359
                     /gene="VIL1"
                     /gene_synonym="D2S1471; VIL"
     regulatory      4921..4926
                     /regulatory_class="polyA_signal_sequence"
                     /gene="VIL1"
                     /gene_synonym="D2S1471; VIL"
                     /note="hexamer: AATAAA"
     polyA_site      4938
                     /gene="VIL1"
                     /gene_synonym="D2S1471; VIL"
ORIGIN      
        1 acaattccct gagatctccc aggtggcagc tgcctcccca agacaggctc actcaccatg
       61 accaagctga gcgcccaagt caaaggctct ctcaacatca ccaccccggg gctgcagata
      121 tggaggatcg aggccatgca gatggtgcct gttccttcca gcacctttgg aagcttcttc
      181 gatggtgact gctacatcat cctggctatc cacaagacag ccagcagcct gtcctatgac
      241 atccactact ggattggcca ggactcatcc ctggatgagc agggggcagc tgccatctac
      301 accacacaga tggatgactt cctgaagggc cgggctgtgc agcaccgcga ggtccagggc
      361 aacgagagcg aggccttccg aggctacttc aagcaaggcc ttgtgatccg gaaagggggc
      421 gtggcttctg gcatgaagca cgtggagacc aactcctatg acgtccagag gctgctgcat
      481 gtcaagggca agaggaacgt ggtagctgga gaggtagaga tgtcctggaa gagtttcaac
      541 cgaggggatg ttttcctcct ggaccttggg aagcttatca tccagtggaa tggaccggaa
      601 agcacccgta tggagagact caggggcatg actctggcca aggagatccg agaccaggag
      661 cggggagggc gcacctatgt aggcgtggtg gacggagaga atgaattggc atccccgaag
      721 ctgatggagg tgatgaacca cgtgctgggc aagcgcaggg agctgaaggc ggccgtgccc
      781 gacacggtgg tggagccggc actcaaggct gcactcaaac tgtaccatgt gtctgactcc
      841 gaggggaatc tggtggtgag ggaagtcgcc acacggccac tgacacagga cctgctcagt
      901 cacgaggact gttacatcct ggaccagggg ggcctgaaga tctacgtgtg gaaagggaag
      961 aaagccaatg agcaggagaa gaagggagcc atgagccatg cgctgaactt catcaaagcc
     1021 aagcagtacc caccaagcac acaggtggag gtgcagaatg atggggctga gtcggccgtc
     1081 tttcagcagc tcttccagaa gtggacagcg tccaaccgga cctcaggcct aggcaaaacc
     1141 cacactgtgg gctccgtggc caaagtggaa caggtgaagt tcgatgccac atccatgcat
     1201 gtcaagcctc aggtggctgc ccagcagaag atggtagatg atgggagtgg ggaagtgcag
     1261 gtgtggcgca ttgagaacct agagctggta cctgtggatt ccaagtggct aggccacttc
     1321 tatgggggcg actgctacct gctgctctac acctacctca tcggcgagaa gcagcattac
     1381 ctgctctacg tttggcaggg cagccaggcc agccaagatg aaattacagc atcagcttat
     1441 caagccgtca tcctggacca gaagtacaat ggtgaaccag tccagatccg ggtcccaatg
     1501 ggcaaggagc cacctcatct tatgtccatc ttcaagggac gcatggtggt ctaccaggga
     1561 ggcacctccc gaactaacaa cttggagacc gggccctcca cacggctgtt ccaggtccag
     1621 ggaactggcg ccaacaacac caaggccttt gaggtcccag cgcgggccaa tttcctcaat
     1681 tccaatgatg tctttgtcct caagacccag tcttgctgct atctatggtg tgggaagggt
     1741 tgtagcgggg acgagcggga gatggccaag atggttgctg acaccatctc ccggacggag
     1801 aagcaagtgg tggtggaagg gcaggagcca gccaacttct ggatggccct gggtgggaag
     1861 gccccctatg ccaacaccaa gagactacag gaagaaaacc tggtcatcac cccccggctc
     1921 tttgagtgtt ccaacaagac tgggcgcttc ctggccacag agatccctga cttcaatcag
     1981 gatgacttgg aagaggatga tgtgttccta ctagatgtct gggaccaggt cttcttctgg
     2041 attgggaaac atgccaacga ggaggagaag aaggccgcag caaccactgc acaggaatac
     2101 ctcaagaccc atcccagcgg gcgtgaccct gagaccccca tcattgtggt gaagcaggga
     2161 cacgagcccc ccaccttcac aggctggttc ctggcttggg atcccttcaa gtggagtaac
     2221 accaaatcct atgaggacct gaaggcggag cttggcaact ctagggactg gagccagatc
     2281 actgctgagg tcacaagccc caaagtggac gtgttcaatg ctaacagcaa cctcagttct
     2341 gggcctctgc ccatcttccc cctggagcag ctagtgaaca agcctgtaga ggagctcccc
     2401 gagggtgtgg accccagcag gaaggaggaa cacctgtcca ttgaagattt cactcaggcc
     2461 tttgggatga ctccagctgc cttctctgct ctgcctcgat ggaagcaaca aaacctcaag
     2521 aaagaaaaag gactattttg agaagagtag ctgtggttgt aaagcagtac cctaccctga
     2581 ttgtagggtc tcattttctc accgatatta gtcctacacc aattgaagtg aaattttgca
     2641 gatgtgccta tgagcacaaa cttctgtggc aaatgccagt tttgtttaat aatgtaccta
     2701 ttccttcaga aagatgatac cccaaaagga gcctatggtc ctcatttcaa cttctaaggt
     2761 cgctagattg tttctatcct gaggtattgc atcaatttta atactcctat agttttctct
     2821 tcttagaaga gcacaaacac tccatggaac attagagttc tgaggcacta ccctagcttg
     2881 tcctctatca tgactcattt ttatctatgg caggtaggct gaagcacttt gcaggtttac
     2941 atcttcccca gagtaacagc ttttcctttt cacatatact ttccttactg ccttactcag
     3001 tgggtaagtt aaagggctga aggagagttg aatggtccac aagactaccc tcttaagagg
     3061 tttcacaaat tccaaacagt accagtgaga gcagcacttc cactggggct aggcttgaga
     3121 cctaaaggca agtatgaaat gcatatgcta cttcactccc tctcccaacc cttaataatg
     3181 aggcaaagca agagcctagt gaaggccaat gctaggttta caaacttacc cagaagcctc
     3241 tgcaaagctt cacaggctcc tcagatgaaa ataacaggaa tcaatgggga ctacggccag
     3301 acactggttt gccattctgt tccttttaag aagtaacagt gctgcaagga agtccatgtc
     3361 agaaagccaa cagaaggtga tttccacaac tttgaacagg ttgttacaag tatcagcaag
     3421 aatgtgtcct tttcagaaat aacagtcaaa tcaaagaagg ttaataaagg ctttaatttc
     3481 atacacacaa aaaaactcta tgcataattt aaaaaggaaa caaaaacaaa gaaaaaccgt
     3541 aaaggataca gaggaacagt tctgctaaaa cacagataaa agtgccgctc catacaaaac
     3601 ataaagaatc agaatcaaaa gtcactctga acataaagaa aaaaaatcat ctcacaaata
     3661 atgtggccac agctgccaga aaacctggta gtggctcaat taggcaaagt gtaggaatct
     3721 catttttgtt tttctctcct taagtttaaa gaaacaacaa tgacaatagg ccagagaagt
     3781 tagggaggga aagaaaagct caaagggagg gaaacctggg gacaagaggt gtgcacaccc
     3841 acatgtggtc tcactcttca cacaggccca ctatttttga agtagaccag tttagttgac
     3901 tgttcttctt tgttctggca tctgactgga ccaacctgga acctggtcca gaccctcacc
     3961 cactctattc ttatgccaat ggacatacct atactttgaa cctctgtact tttaagaaaa
     4021 gtccaatgtt acaaaatcaa atgcttatat tcagactggc acacttttta aataaaaact
     4081 ccatacacct cagacatata gcacacatgg agacaactta ctaattgtgt gtaagtatga
     4141 tacaatgaat gagactgcct gaagtctagt aatcaaagca tgccataagg tgaatgattg
     4201 tggttaaaca cagcaaaata attgtcacaa aactttcaag gcctaacaaa ttagaatttt
     4261 ccaataaaaa atatatattt tttcagatgt taataagaca tatcagtaga gacaaaatta
     4321 ggattttgaa gtaatgcaat aaaaagatgt tggagggcag aagtctattt agtttttgta
     4381 tacacttgca agagtgcatt actcagtata aagcaaaatg gggaggaaaa agacatccat
     4441 ccattttatt ggaacacttt tatgtgactt gaatctggtg ttaggttgtt gatttttcta
     4501 aaaatctcct atatatacaa aatccatatg tacttggaga tccagctgtt gccccctgtt
     4561 taaaacaaaa gaccacctcg gggggtcaat taaattaaaa aggccctcca accaccctaa
     4621 atgggataac taagagtatc tactgcagtc atttcagagg acagagaagg aaaatatttt
     4681 aatttgcttt aatataacct cttttcagta gatcacaaat gagtttacaa actacttttt
     4741 tttctcttta atttaggtgt ttgcagataa ttttcattat atccgtagct gtatgtgtgt
     4801 atagttacat aatggtaact acacacgata cagaagaatc agtaaattca tggattattt
     4861 tgctgaggtt ttaaatttta aagcctctgt ttcagaattt tatacttgat caaggagaaa
     4921 aataaatgtg tagtctaaca tttgctttct ggagttaatt aactgatctg taagaaccac
     4981 tgcatatgtc ttaaaatgta aacatattta catttgttgt atttgttatt gagcctttaa
     5041 ggttaggccc agaatgaaca gaccatagca agtaaacaaa taatttttag aatcaaagta
     5101 ttaatagaag accagttcat ggatttgctt attctatcct gctgagacaa aactcatgag
     5161 tgtgcacaca catgtgaata tatccctacg aaacagtcta tcttctcata ggcttaaatt
     5221 atagtcatgg ctattaaaga aattaacagc atccagccac atgcaacttt ccaaccttca
     5281 gtactattag gtgattaaaa tcaacaaata tgaagtttag ttcatttttc ccttaaaatt
     5341 ccaacaaaga tcaaaaggtt gtatctagaa ctaattgcca tcaagttcca attcaatgtc
     5401 atttaaagta atgtaaccac acatttggta ttttcaatag gaaggttaat taggtattgt
     5461 gcaaactgcc tgcaacggaa gcactcagtc cttatctaac ttgtcccttc ctggccccaa
     5521 catgctaact gccccatccc caattctggg caaactgatc acaatgtgca aaaaataata
     5581 tattatctat ttataatttt aaaatatata cagccagctc aaaaacaaca acaaacccca
     5641 tcaacatagt ccagctgaaa tctccactgg tagtcaaaga agtagattaa aggagtaaag
     5701 gaaggagaag gctgataggg ccacaagaat ggacagacat caagtaaaat ttgagtccca
     5761 aacatgcaag tacatgagca gtgagataac ttatatattc caataaccta ttttcaacaa
     5821 ctcctgccca agacatgaag atcaaatcag gtttctgagg taagtgtact tctaaaccat
     5881 acacacatga aagctatgaa agcaattcaa atgaggctgc ttcatgaggc aatctagact
     5941 tatggcatta tttctacttt tctccatgtt ttaattgcag cctgcacttt aaatctttcc
     6001 caataatttt taacagtgcc tctcaaatgc aaagacacgt aaaagaatta ataactagcc
     6061 aaagaatttt atcaccgctt cactcattta taagaaaacc aattatttcc aagcaaaatc
     6121 aaaccaaacc aaagaggctc ctggtagaaa tgaaacaaaa ctttaaagct agttttaaaa
     6181 caaatatttt cctctgctct aaactactct ggcgttttct accactctac cattttggaa
     6241 cattcattac aataaggtat ataggtagat ggtaggaggc aaagcattta tcagtagttg
     6301 agcaaaactg ctgaggccat ttataattca aagaatgaaa acttagaata gtttactaca
     6361 ttagaataca tccaagttcc aagagtagga ctggagctct cttaaggcaa tctttcagaa
     6421 aagatgttgc atcttctgtg atgatggttt gtgttttttt tgtttttgtt ttcgtttttt
     6481 ttaatgagat ggagtctcac
//
