LOCUS       NM_024558               1772 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens valosin containing protein lysine methyltransferase
            (VCPKMT), transcript variant 1, mRNA.
ACCESSION   NM_024558
VERSION     NM_024558.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1772)
  AUTHORS   Petrovic,S., Roske,Y., Rami,B., Phan,M.H.Q., Panakova,D. and
            Heinemann,U.
  TITLE     Structural remodeling of AAA+ ATPase p97 by adaptor protein ASPL
            facilitates posttranslational methylation by METTL21D
  JOURNAL   Proc Natl Acad Sci U S A 120 (4), e2208941120 (2023)
   PUBMED   36656859
  REMARK    GeneRIF: Structural remodeling of AAA+ ATPase p97 by adaptor
            protein ASPL facilitates posttranslational methylation by METTL21D.
REFERENCE   2  (bases 1 to 1772)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   3  (bases 1 to 1772)
  AUTHORS   Cloutier,P., Lavallee-Adam,M., Faubert,D., Blanchette,M. and
            Coulombe,B.
  TITLE     A newly uncovered group of distantly related lysine
            methyltransferases preferentially interact with molecular
            chaperones to regulate their activity
  JOURNAL   PLoS Genet 9 (1), e1003210 (2013)
   PUBMED   23349634
  REMARK    GeneRIF: METTL21D trimethylates Lys-315 in VCP/p97 in a
            ASPCR1-dependent manner
REFERENCE   4  (bases 1 to 1772)
  AUTHORS   Kernstock,S., Davydova,E., Jakobsson,M., Moen,A., Pettersen,S.,
            Maelandsmo,G.M., Egge-Jacobsen,W. and Falnes,P.O.
  TITLE     Lysine methylation of VCP by a member of a novel human protein
            methyltransferase family
  JOURNAL   Nat Commun 3, 1038 (2012)
   PUBMED   22948820
  REMARK    GeneRIF: METTL21D is the methyltransferase responsible for lysine
            methylation of VCP.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK023982.1, BI753530.1 and AL109758.3.
            
            On Nov 22, 2018 this sequence version replaced NM_024558.2.
            
            Transcript Variant: This variant (1) encodes the longer isoform
            (A).
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR3476690.627834.1,
                                           SRR1660807.208294.1 [ECO:0000332]
            RNAseq introns              :: mixed/partial sample support
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000350]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000395860.7/ ENSP00000379201.2
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-13                AK023982.1         8-20
            14-731              BI753530.1         1-718
            732-1772            AL109758.3         47795-48835         c
FEATURES             Location/Qualifiers
     source          1..1772
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="14"
                     /map="14q21.3"
     gene            1..1772
                     /gene="VCPKMT"
                     /gene_synonym="C14orf138; METTL21D; VCP-KMT"
                     /note="valosin containing protein lysine
                     methyltransferase"
                     /db_xref="GeneID:79609"
                     /db_xref="HGNC:HGNC:20352"
                     /db_xref="MIM:615260"
     exon            1..286
                     /gene="VCPKMT"
                     /gene_synonym="C14orf138; METTL21D; VCP-KMT"
                     /inference="alignment:Splign:2.1.0"
     CDS             21..710
                     /gene="VCPKMT"
                     /gene_synonym="C14orf138; METTL21D; VCP-KMT"
                     /note="isoform a is encoded by transcript variant 1;
                     methyltransferase-like protein 21D; VCP lysine
                     methyltransferase; protein-lysine methyltransferase
                     METTL21D; methyltransferase like 21D; valosin containing
                     protein lysine (K) methyltransferase; protein N-lysine
                     methyltransferase METTL21D"
                     /codon_start=1
                     /product="protein N-lysine methyltransferase METTL21D
                     isoform a"
                     /protein_id="NP_078834.2"
                     /db_xref="CCDS:CCDS9696.2"
                     /db_xref="GeneID:79609"
                     /db_xref="HGNC:HGNC:20352"
                     /db_xref="MIM:615260"
                     /translation="MADTLESSLEDPLRSFVRVLEKRDGTVLRLQQYSSGGVGCVVWD
                     AAIVLSKYLETPEFSGDGAHALSRRSVLELGSGTGAVGLMAATLGADVVVTDLEELQD
                     LLKMNINMNKHLVTGSVQAKVLKWGEEIEGFPSPPDFILMADCIYYEESLEPLLKTLK
                     DISGFETCIICCYEQRTMGKNPEIEKKYFELLQLDFDFEKIPLEKHDEEYRSEDIHII
                     YIRKKKSKFPS"
     exon            287..397
                     /gene="VCPKMT"
                     /gene_synonym="C14orf138; METTL21D; VCP-KMT"
                     /inference="alignment:Splign:2.1.0"
     exon            398..470
                     /gene="VCPKMT"
                     /gene_synonym="C14orf138; METTL21D; VCP-KMT"
                     /inference="alignment:Splign:2.1.0"
     exon            471..590
                     /gene="VCPKMT"
                     /gene_synonym="C14orf138; METTL21D; VCP-KMT"
                     /inference="alignment:Splign:2.1.0"
     exon            591..695
                     /gene="VCPKMT"
                     /gene_synonym="C14orf138; METTL21D; VCP-KMT"
                     /inference="alignment:Splign:2.1.0"
     exon            696..1772
                     /gene="VCPKMT"
                     /gene_synonym="C14orf138; METTL21D; VCP-KMT"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1753..1758
                     /regulatory_class="polyA_signal_sequence"
                     /gene="VCPKMT"
                     /gene_synonym="C14orf138; METTL21D; VCP-KMT"
                     /note="hexamer: AATAAA"
     polyA_site      1772
                     /gene="VCPKMT"
                     /gene_synonym="C14orf138; METTL21D; VCP-KMT"
                     /note="major polyA site"
ORIGIN      
        1 gctttctgtt gccgggcgca atggcggata cgctggagtc ctcgctggag gacccactgc
       61 ggagctttgt gcgagttttg gagaagcggg atggtacagt gctacgacta cagcagtata
      121 gctccggtgg cgtgggttgc gttgtgtggg acgctgccat tgtcctttct aaatacctgg
      181 aaacgcccga gttttctggc gacggggccc acgcgctgag ccggcggtcg gtgctggagc
      241 tgggttcggg caccggggcc gtggggctca tggctgctac cctcggggct gatgttgtag
      301 tcaccgatct tgaggaattg caagacttgc tgaagatgaa tattaatatg aacaagcatc
      361 ttgtcactgg ttctgttcaa gccaaggtac tgaaatgggg ggaagaaata gaaggctttc
      421 cttctccacc cgacttcata ctgatggccg actgcatata ctatgaagag tctttggagc
      481 cattgctgaa aactctaaaa gatatcagcg gatttgaaac ttgtattata tgttgttatg
      541 aacaacgaac aatggggaaa aatccagaaa ttgagaaaaa atattttgag ctccttcagc
      601 tagattttga ctttgaaaaa attcctttgg aaaaacatga tgaagagtat cgaagtgaag
      661 atattcatat tatatacatc agaaagaaaa aatcgaaatt tccatcgtga agcctttaat
      721 catcttaccc aaggctctaa caacctgggt aaactaaaga tgtgaataga gcatgtgaat
      781 acagcatggg aagattgtgt tcacagattt ttttttccgg cacgtcctta gagatccgat
      841 gtatagatga tgaccaccag gggctgtctg caatacgaaa aattcctgct tgcctgcctg
      901 cctgccaatg gccctgaatc cagcttaggt tacttagttc agcatcaagt tcttcttaaa
      961 tgttgggaat cagttattca aataaaaatt ggtattgagg cgaggctgaa atctgccaaa
     1021 aacagccaac atgatttgac tggacctttt acaggaagct agagataaat ttctgaagag
     1081 aactatctgc tattatataa taatgtttta aattcaaact agtaaatttt agtttgtctt
     1141 cagagtttaa aaggttttca ttttgtacat aattatacaa tatttatcat ttgtattttc
     1201 cacttcattt cttttaaaat atctttaata ctgaaatgtt cttttaattt taaaaagaac
     1261 tgagatattg tcttgtatac ttatattagc caaagttttc ttttcctcca ccatacatat
     1321 ctatgtgatt taatcagtaa attgtactgt aatgagttgc taagaagaca aatcagaaga
     1381 ttggaggaac aaaagatatc tttacagatt tttcatttga gcatggagtg agaatagaaa
     1441 gaccagtttc aggcttatgc acttacgtgg ctcatgcact ttatgtatat gttccaggaa
     1501 aatctggctt aaaaatactg gtattgttta catgaagcag tgaaaggttt ttgaataact
     1561 acaaatgtag ttctatatgt atataccaaa tgaatttctg ttctgtgtct ctctgtttta
     1621 tgttatgaag ccattcgctc atatacaata atctgtcaag gactttaatc atacttgttc
     1681 caaagagtag taaccggatg gaattctggt atttataggc attggtgcta gatggtacat
     1741 tttatgtgtt aaaataaaca ttgtttttga ga
//
