LOCUS       NM_025054               9956 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens valosin containing protein interacting protein 1
            (VCPIP1), mRNA.
ACCESSION   NM_025054
VERSION     NM_025054.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 9956)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   2  (bases 1 to 9956)
  AUTHORS   Huang,J., Zhou,Q., Gao,M., Nowsheen,S., Zhao,F., Kim,W., Zhu,Q.,
            Kojima,Y., Yin,P., Zhang,Y., Guo,G., Tu,X., Deng,M., Luo,K.,
            Qin,B., Machida,Y. and Lou,Z.
  TITLE     Tandem Deubiquitination and Acetylation of SPRTN Promotes
            DNA-Protein Crosslink Repair and Protects against Aging
  JOURNAL   Mol Cell 79 (5), 824-835 (2020)
   PUBMED   32649882
REFERENCE   3  (bases 1 to 9956)
  AUTHORS   Tsai,Y.C., Kotiya,A., Kiris,E., Yang,M., Bavari,S., Tessarollo,L.,
            Oyler,G.A. and Weissman,A.M.
  TITLE     Deubiquitinating enzyme VCIP135 dictates the duration of botulinum
            neurotoxin type A intoxication
  JOURNAL   Proc Natl Acad Sci U S A 114 (26), E5158-E5166 (2017)
   PUBMED   28584101
  REMARK    GeneRIF: BoNT/A catalytic light chain binds to, and is a substrate
            for, the ubiquitin ligase HECTD2. However, the light chain evades
            proteasomal degradation by the dominant effect of a
            deubiquitinating enzyme, VCIP135/VCPIP1. This deubiquitinating
            enzyme binds BoNT/A light chain directly.
REFERENCE   4  (bases 1 to 9956)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Paulo,J.A., Cannon,J.R., Ting,L.,
            Baltier,K., Colby,G., Gebreab,F., Gygi,M.P., Parzen,H., Szpyt,J.,
            Tam,S., Zarraga,G., Pontano-Vaites,L., Swarup,S., White,A.E.,
            Schweppe,D.K., Rad,R., Erickson,B.K., Obar,R.A., Guruharsha,K.G.,
            Li,K., Artavanis-Tsakonas,S., Gygi,S.P. and Harper,J.W.
  TITLE     Architecture of the human interactome defines protein communities
            and disease networks
  JOURNAL   Nature 545 (7655), 505-509 (2017)
   PUBMED   28514442
REFERENCE   5  (bases 1 to 9956)
  AUTHORS   Zhang,X. and Wang,Y.
  TITLE     Cell cycle regulation of VCIP135 deubiquitinase activity and
            function in p97/p47-mediated Golgi reassembly
  JOURNAL   Mol Biol Cell 26 (12), 2242-2251 (2015)
   PUBMED   25904330
  REMARK    GeneRIF: Phosphorylation and ubiquitination are coordinated via
            VCIP135 to control Golgi membrane dynamics in the cell cycle.
REFERENCE   6  (bases 1 to 9956)
  AUTHORS   Beausoleil,S.A., Jedrychowski,M., Schwartz,D., Elias,J.E.,
            Villen,J., Li,J., Cohn,M.A., Cantley,L.C. and Gygi,S.P.
  TITLE     Large-scale characterization of HeLa cell nuclear phosphoproteins
  JOURNAL   Proc Natl Acad Sci U S A 101 (33), 12130-12135 (2004)
   PUBMED   15302935
REFERENCE   7  (bases 1 to 9956)
  AUTHORS   Wang,Y., Satoh,A., Warren,G. and Meyer,H.H.
  TITLE     VCIP135 acts as a deubiquitinating enzyme during p97-p47-mediated
            reassembly of mitotic Golgi fragments
  JOURNAL   J Cell Biol 164 (7), 973-978 (2004)
   PUBMED   15037600
  REMARK    Erratum:[J Cell Biol. 2004 Aug 2;166(3):433. Wang, Yangzhuang
            [corrected to Wang, Yanzhuang]]
REFERENCE   8  (bases 1 to 9956)
  AUTHORS   Ficarro,S., Chertihin,O., Westbrook,V.A., White,F., Jayes,F.,
            Kalab,P., Marto,J.A., Shabanowitz,J., Herr,J.C., Hunt,D.F. and
            Visconti,P.E.
  TITLE     Phosphoproteome analysis of capacitated human sperm. Evidence of
            tyrosine phosphorylation of a kinase-anchoring protein 3 and
            valosin-containing protein/p97 during capacitation
  JOURNAL   J Biol Chem 278 (13), 11579-11589 (2003)
   PUBMED   12509440
  REMARK    GeneRIF: evidence of tyrosine phosphorylation during sperm
            capacitation
REFERENCE   9  (bases 1 to 9956)
  AUTHORS   Uchiyama,K., Jokitalo,E., Kano,F., Murata,M., Zhang,X., Canas,B.,
            Newman,R., Rabouille,C., Pappin,D., Freemont,P. and Kondo,H.
  TITLE     VCIP135, a novel essential factor for p97/p47-mediated membrane
            fusion, is required for Golgi and ER assembly in vivo
  JOURNAL   J Cell Biol 159 (5), 855-866 (2002)
   PUBMED   12473691
REFERENCE   10 (bases 1 to 9956)
  AUTHORS   Simpson,J.C., Wellenreuther,R., Poustka,A., Pepperkok,R. and
            Wiemann,S.
  TITLE     Systematic subcellular localization of novel proteins identified by
            large-scale cDNA sequencing
  JOURNAL   EMBO Rep 1 (3), 287-292 (2000)
   PUBMED   11256614
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC090154.19.
            
            On Nov 22, 2018 this sequence version replaced NM_025054.4.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: CR936659.1, SRR1660807.67178.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000310421.5/ ENSP00000309031.4
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2983              AC090154.19        45763-48745         c
            2984-3070           AC090154.19        32972-33058         c
            3071-9956           AC090154.19        10001-16886         c
FEATURES             Location/Qualifiers
     source          1..9956
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="8"
                     /map="8q13.1"
     gene            1..9956
                     /gene="VCPIP1"
                     /gene_synonym="DUBA3; VCIP135"
                     /note="valosin containing protein interacting protein 1"
                     /db_xref="GeneID:80124"
                     /db_xref="HGNC:HGNC:30897"
                     /db_xref="MIM:611745"
     exon            1..2983
                     /gene="VCPIP1"
                     /gene_synonym="DUBA3; VCIP135"
                     /inference="alignment:Splign:2.1.0"
     CDS             274..3942
                     /gene="VCPIP1"
                     /gene_synonym="DUBA3; VCIP135"
                     /EC_number="3.4.19.12"
                     /note="deubiquitinating protein VCIP135;
                     valosin-containing protein p97/p47 complex-interacting
                     protein 1; valosin-containing protein p97/p47
                     complex-interacting protein p135; VCP/p47
                     complex-interacting 135-kDa protein"
                     /codon_start=1
                     /product="deubiquitinating protein VCPIP1"
                     /protein_id="NP_079330.2"
                     /db_xref="CCDS:CCDS6192.1"
                     /db_xref="GeneID:80124"
                     /db_xref="HGNC:HGNC:30897"
                     /db_xref="MIM:611745"
                     /translation="MSQPPPPPPPLPPPPPPPEAPQTPSSLASAAASGGLLKRRDRRI
                     LSGSCPDPKCQARLFFPASGSVSIECTECGQRHEQQQLLGVEEVTDPDVVLHNLLRNA
                     LLGVTGAPKKNTELVKVMGLSNYHCKLLSPILARYGMDKQTGRAKLLRDMNQGELFDC
                     ALLGDRAFLIEPEHVNTVGYGKDRSGSLLYLHDTLEDIKRANKSQECLIPVHVDGDGH
                     CLVHAVSRALVGRELFWHALRENLKQHFQQHLARYQALFHDFIDAAEWEDIINECDPL
                     FVPPEGVPLGLRNIHIFGLANVLHRPIILLDSLSGMRSSGDYSATFLPGLIPAEKCTG
                     KDGHLNKPICIAWSSSGRNHYIPLVGIKGAALPKLPMNLLPKAWGVPQDLIKKYIKLE
                     EDGGCVIGGDRSLQDKYLLRLVAAMEEVFMDKHGIHPSLVADVHQYFYRRTGVIGVQP
                     EEVTAAAKKAVMDNRLHKCLLCGALSELHVPPEWLAPGGKLYNLAKSTHGQLRTDKNY
                     SFPLNNLVCSYDSVKDVLVPDYGMSNLTACNWCHGTSVRKVRGDGSIVYLDGDRTNSR
                     STGGKCGCGFKHFWDGKEYDNLPEAFPITLEWGGRVVRETVYWFQYESDSSLNSNVYD
                     VAMKLVTKHFPGEFGSEILVQKVVHTILHQTAKKNPDDYTPVNIDGAHAQRVGDVQGQ
                     ESESQLPTKIILTGQKTKTLHKEELNMSKTERTIQQNITEQASVMQKRKTEKLKQEQK
                     GQPRTVSPSTIRDGPSSAPATPTKAPYSPTTSKEKKIRITTNDGRQSMVTLKSSTTFF
                     ELQESIAREFNIPPYLQCIRYGFPPKELMPPQAGMEKEPVPLQHGDRITIEILKSKAE
                     GGQSAAAHSAHTVKQEDIAVTGKLSSKELQEQAEKEMYSLCLLATLMGEDVWSYAKGL
                     PHMFQQGGVFYSIMKKTMGMADGKHCTFPHLPGKTFVYNASEDRLELCVDAAGHFPIG
                     PDVEDLVKEAVSQVRAEATTRSRESSPSHGLLKLGSGGVVKKKSEQLHNVTAFQGKGH
                     SLGTASGNPHLDPRARETSVVRKHNTGTDFSNSSTKTEPSVFTASSSNSELIRIAPGV
                     VTMRDGRQLDPDLVEAQRKKLQEMVSSIQASMDRHLRDQSTEQSPSDLPQRKTEVVSS
                     SAKSGSLQTGLPESFPLTGGTENLNTETTDGCVADALGAAFATRSKAQRGNSVEELEE
                     MDSQDAEMTNTTEPMDHS"
     exon            2984..3070
                     /gene="VCPIP1"
                     /gene_synonym="DUBA3; VCIP135"
                     /inference="alignment:Splign:2.1.0"
     exon            3071..9956
                     /gene="VCPIP1"
                     /gene_synonym="DUBA3; VCIP135"
                     /inference="alignment:Splign:2.1.0"
     regulatory      8158..8163
                     /regulatory_class="polyA_signal_sequence"
                     /gene="VCPIP1"
                     /gene_synonym="DUBA3; VCIP135"
                     /note="hexamer: AATAAA"
     polyA_site      8190
                     /gene="VCPIP1"
                     /gene_synonym="DUBA3; VCIP135"
                     /note="major polyA site"
     regulatory      8334..8339
                     /regulatory_class="polyA_signal_sequence"
                     /gene="VCPIP1"
                     /gene_synonym="DUBA3; VCIP135"
                     /note="hexamer: AATAAA"
     polyA_site      8357
                     /gene="VCPIP1"
                     /gene_synonym="DUBA3; VCIP135"
     regulatory      8470..8475
                     /regulatory_class="polyA_signal_sequence"
                     /gene="VCPIP1"
                     /gene_synonym="DUBA3; VCIP135"
                     /note="hexamer: AATAAA"
     polyA_site      8494
                     /gene="VCPIP1"
                     /gene_synonym="DUBA3; VCIP135"
                     /note="major polyA site"
     regulatory      9930..9935
                     /regulatory_class="polyA_signal_sequence"
                     /gene="VCPIP1"
                     /gene_synonym="DUBA3; VCIP135"
                     /note="hexamer: ATTAAA"
     polyA_site      9956
                     /gene="VCPIP1"
                     /gene_synonym="DUBA3; VCIP135"
                     /note="major polyA site"
ORIGIN      
        1 gtgtctgagg gagagagagc gagagtgagt gagtgtgagt gcggggtggg gtggtggccg
       61 ttacgttcgg ggcaacggct acggcagtgg agaagtaaga agagaaacaa cgtccggcgc
      121 ttccgccttc gcttaggagg aggaagagct ggtagggaaa ggaaagtgct tcgccctggg
      181 cctggactgg accgagtcgg gttggtgggg gtctgggcta tgagcttttg agggtcgcct
      241 gggacgcgga gcgagacacg agagccagga gctatgtctc agccgccgcc gccgccgcct
      301 ccgttgccgc cgccacctcc tccccctgag gctccacaga ctccgtcgtc cttggcgtcg
      361 gcggctgctt cgggggggct tttgaagcgg agagaccgga gaatcctttc cgggagctgc
      421 ccggatccga agtgtcaggc gcgtctattt ttcccggcct ccggttctgt cagcatcgag
      481 tgtaccgagt gcggccagcg gcacgagcag caacagctgc tgggggttga ggaggtgacc
      541 gacccggacg tagtgctaca caacctgctg cggaacgcgc tgctcggggt tacgggggca
      601 cccaagaaga acacggaact ggtaaaggtg atgggccttt ccaactatca ctgcaaattg
      661 ttgtcgccca tattagctcg ctatggaatg gacaaacaga caggccgggc caagcttctc
      721 cgggacatga accagggcga actgttcgat tgcgccttac tgggtgaccg cgccttcctc
      781 atagaaccag agcatgttaa cactgtgggc tatggcaagg accgctccgg aagcctcctg
      841 tatttgcatg acactctgga ggacattaag cgggccaata aaagccagga atgtctcatt
      901 ccagtgcatg tggacgggga tggacactgc ttggtgcatg ctgtgtctcg ggctctagta
      961 ggccgagagc tcttctggca tgccttaaga gagaatctta aacagcactt tcagcagcac
     1021 ctggcccgat atcaagctct gttccatgac ttcattgatg ctgctgagtg ggaggacatt
     1081 atcaatgagt gtgaccctct gtttgtacca cctgagggtg ttcccttggg cctgaggaat
     1141 atccacatat ttggtcttgc caatgtgcta catcgtccta ttattctgtt agattccctc
     1201 agtggcatga gaagctctgg tgattattca gccacctttc tacctgggct catccctgca
     1261 gagaagtgca ctgggaaaga tggtcatttg aacaaaccaa tctgtattgc atggagcagc
     1321 tccggtagaa accattatat ccccttggta ggcataaaag gggctgcttt gcccaaactg
     1381 cctatgaatt tgcttcctaa agcatggggt gtgcctcagg accttattaa aaagtacata
     1441 aaacttgaag aggatggtgg ttgtgttatt ggaggtgaca gaagtttgca agataaatac
     1501 ttacttaggc ttgttgctgc tatggaagaa gtctttatgg acaaacatgg tatccatcct
     1561 agtttggttg ctgatgtcca tcagtatttc tacagaagga ctggagtgat aggagttcag
     1621 cctgaggaag tcacagcagc tgctaaaaaa gcagtaatgg ataatcgcct tcacaaatgt
     1681 ttgctctgtg gtgccctttc tgaacttcat gttcctccag agtggttggc tcctggaggg
     1741 aaattgtata acctggcaaa aagtactcat ggacagctga ggactgacaa aaattacagc
     1801 tttcccttga acaatttggt ttgctcatat gattcagtga aagatgttct ggtaccagac
     1861 tatggaatga gtaacctaac agcttgtaat tggtgccatg gcacatctgt gcgaaaggtc
     1921 agaggagatg ggtctattgt gtatttggat ggagacagaa ctaattctag gtccactggt
     1981 ggcaaatgtg gttgtggatt caaacacttt tgggatggta aggagtatga caatctacca
     2041 gaagctttcc ctattacttt agaatggggt ggaagagtgg tcagagaaac agtatattgg
     2101 ttccagtatg aaagtgattc atctttgaat agtaatgttt acgatgttgc aatgaaactt
     2161 gttaccaagc actttccagg tgaatttggg agtgaaatcc tagttcagaa agttgtccac
     2221 actatattgc atcagactgc caaaaagaat cccgatgatt atactcctgt aaatatagat
     2281 ggtgctcacg cccaaagagt tggagatgtt caaggacaag aatcagagtc tcagctccca
     2341 actaaaatta ttcttactgg acagaaaaca aaaactttgc acaaggagga gttaaacatg
     2401 agtaaaactg aaagaactat tcaacagaat attacggaac aggcttctgt aatgcagaaa
     2461 cggaaaacag agaagttaaa acaagaacaa aaagggcaac ccaggactgt ttctcccagt
     2521 accattcgtg atggtccatc ctctgcacct gctacaccta ccaaggctcc ctattcaccg
     2581 acaacttcta aggagaagaa gatccgaatc acaactaatg atggacgaca gtccatggtt
     2641 acccttaagt cttcaacaac cttttttgaa cttcaggaaa gtatagccag agaattcaac
     2701 attcctccat atttacagtg tattcgatac gggtttcctc ctaaagagtt aatgccacca
     2761 caggcaggaa tggaaaagga accagttcct ttacagcatg gcgacagaat tacaatagaa
     2821 attctaaaaa gtaaagctga aggtggtcag tctgctgcag cacactcagc ccacactgtg
     2881 aaacaagaag atattgctgt tactggtaaa ctgtcatcta aggaacttca ggagcaagct
     2941 gaaaaagaaa tgtactcctt gtgtctttta gcaacattaa tgggagaaga tgtatggtct
     3001 tatgcaaagg gacttcctca catgtttcag cagggtggtg tattctacag tattatgaag
     3061 aaaaccatgg gtatggctga tggcaagcat tgtacttttc cacatctgcc tggcaaaacc
     3121 tttgtctata atgcttctga agatagactg gaattgtgtg tggatgctgc aggacatttc
     3181 cccattggtc ctgatgttga agatttagtt aaagaggctg taagtcaggt tcgagcagag
     3241 gctactacaa gaagtaggga atcaagtccc tcacatgggc tattaaaact aggtagtggt
     3301 ggagtagtga aaaagaaatc tgagcaactt cataacgtaa ctgcctttca gggaaaaggg
     3361 cattctttag gaactgcatc tggtaaccca caccttgatc caagagctag ggaaacttca
     3421 gttgtaagaa agcataatac agggacagac tttagtaata gttccactaa aacagagcct
     3481 tctgtattca cagcttcttc tagtaatagt gagcttattc gaatagctcc tggagtagta
     3541 acaatgagag acggcaggca gcttgatcct gatttggttg aggcccagcg aaaaaaattg
     3601 caggaaatgg tttcttctat tcaggcttca atggacaggc accttcggga tcaaagtaca
     3661 gagcagtcac catctgatct tcctcaaagg aaaacagaag ttgtgagttc ttctgcaaag
     3721 tctgggagtc ttcagactgg tttgcctgaa tcttttcctt taactggtgg tactgaaaat
     3781 ttgaatacag aaacaactga tggctgtgta gcagatgcac tgggagcagc ctttgccaca
     3841 aggtcaaaag cacaaagggg aaattccgtg gaggagcttg aagagatgga tagtcaagat
     3901 gctgagatga ctaacacaac tgagccaatg gatcactctt gatttaatta gaggctaata
     3961 aaggcagaat gtttattgtg aatatgtaat atttgttggc tgggccacgt aacttgatta
     4021 gtcattaaaa atcttgtacg tatataattc aaagattata tcttgttatt cagtgcatga
     4081 tagcaagtgt gtgattggcc atagctttta atatactgct gcccaggctg gctctgaatt
     4141 tctataaatt agctgttaga tctgctgaga tgactttctt ccatatatgt ggttcattgg
     4201 aagttctttg taattttaat tccatgaaag tcttaatgtt tccaacatga aaattctctt
     4261 acttaaatac gtttgatttc tgaaaagcct acaaatggag gggaaatgag attgtgctaa
     4321 tgttgatgtc atcaaggtaa ccatctcata tactagtgtc aaaaggttta tgtaaacttg
     4381 aattttatct gaactaaatt gtatcttaaa attcatacaa ttatttatta gacagtggaa
     4441 tgcaaatgtt tctatgaacg taaacacatt catagagatt tcttggcgaa aatatcttga
     4501 gaatagaatg taacgatata acattttgct gatttgtgca agattgggga aaaatttctt
     4561 ctagattgat gaggtcaatg tgattcagtt gtgttgctgt ctgagccaca gccaattgtg
     4621 tgtgctctat taatatgtta ccattatttt tgattcctgg cttggtcaag aacaacctga
     4681 tttttttttt tttaatcttg cagaaaagtt gattgcaaag tgtggtagaa aaagaaataa
     4741 tggtagcagt agagaattta taaataagag tttatcttta tgaatgagtt aattgttcta
     4801 caattttgat cagtggtatt taagcctgta tatgctaagt gtatttatgc ccctttgagc
     4861 caaaattttt tttttttttt cagaatccat aagagtttat ttgttggctt cacttgggtt
     4921 tagatgcaaa ttttactgca tcacaccctg atcagctggg tatttgctta gtgtattcat
     4981 caaatcttca gtgccagttt ccttgagaag cagctttcat gcttattcct taaaaacaac
     5041 agaagagtgt ttatcctgag attggtattg cactatttta ttttattctg caaatctgta
     5101 tcctgaagtg tacatttcaa cccatattct tctctgttca ctgaaattaa gttgaatttg
     5161 aatggctgaa agacaaaata tatgttaata caatctctta cctagagcat tcaaagcctg
     5221 gctgtctctg tttgcacatg taacagatgc agaactgtgt ttgtaattct aagaaaatgt
     5281 atgtcagaaa gtattttatc agatgtttaa aaatctctgc ctgttgattc ataagggata
     5341 tagacagtac tttaacttgt gtttccagat aaggcagatt ttggtcatct ttctgtgaaa
     5401 gtatctctaa ctgaatccac ttcttaaaca aactactcta ttttaaataa ggaaagaatg
     5461 gagaatctag acattttaca ggtgtatctg agcaaaatat atatttgggt tagtttttat
     5521 cacattatca cgtttgtctt tatattttat cttcatccta aattttctcc ctatcaaaac
     5581 agcaggaaat ttttaaactt ttatattgta cactattttt gcataaaata agagctgtag
     5641 ctaccaaata ctatttacta cttagtaaag gtggcttctg tgtattaaac tacgaattac
     5701 aaatatcact tcactaacca ctataattat ttttggcttt ttcagccact ggaacttttt
     5761 ttggggggat ttaaatacag gaaaactgtt taagcaaaaa atgcactgct ttcatgtaaa
     5821 atgatataat cttctttaac aaatattttc ttcattgata actgacagat atctcatagc
     5881 attaacataa aataccagaa acaaacttta tctccctatt cttacattta gtgcaaattg
     5941 gaatgcgtat gtgaatgtta gaagatggac ttaatctctg acaactttat taatttctct
     6001 agctacagca atgctattta aaagccagtt aggtatgaat agtacagtaa tagtttgggt
     6061 tctcccttga taaatgaata aaaggctcaa ttctgataag tgatcaaatt tcttgaaatt
     6121 ctttcaaatc agctgaaaat ttggttgggg tgaattgatg gtttttgaga agtaggccag
     6181 gtatcctcac aaagaagttt tagaataggg tttcttttaa gtttttaaat atcatccttt
     6241 gtctttccat atggttttca taaccaaata catgactata gcatggttat ttttctagta
     6301 ctgccatttg tgatatagcc acatcttggc gtgcaagcta gacatctcag gtaaatttgt
     6361 tttttttttt ttttttagcc ctagaggtaa cattataact aaaatgcatt cctgcaattc
     6421 tgtttgcagt gcttcactaa acattgaaat aaagttgtaa attttttctt cagtaatcca
     6481 ttttatagtt aatcatacca aatttaagtt gatgacctaa agtccaggta gtgttgaaga
     6541 tagtatgtat ctagaatact ggtaatgaga aattgttgta aattgaagaa aacaagatgc
     6601 tgccatatat taagatagaa tataattaac tccatgagaa tggttactca ggaactagtt
     6661 aaagagcaaa cccatttctc ccgcttcact ctgtttcaga tatttttatt ggaaaatgtt
     6721 agggtagagg cctgagaaag ttagcaaatg ctattgactg atactggctc tgtgtcttcc
     6781 agtgattttt agtttggttt ttacagtcaa gcctactgct tgcaattaag atatcaatct
     6841 gaaatgcatc tgtaattcct ggccacaggt ccttattgcc attattttta agtatcttca
     6901 ttggagatga acactgactt ttgcatatgg tctcttacct cagggaagat cacttgctag
     6961 caactcatac tactaatgac aggtttttga gaattgtact gacatcacat gttagctact
     7021 aaatgcaaaa tgtataaaag tattgtttta tgtagttacc taccaatact atctgctact
     7081 gaataactct ctaaatatgt agacctcact aataactaac ctttaggtaa aaaatttact
     7141 tctgtcatta attacacttc aactggtcaa tatagtggca gcataaagtg tttatttcag
     7201 aatatcaata cagttttgtc ttcatttttc aacagtaatt tcaattggtt catacttaaa
     7261 atactacaca gattttgagt tttatggttc tcaaagcaag gtttccatct taagattttt
     7321 ataaacacaa agcctttgca aaattggatt aaattccacc tatcttaatg tgaattaaaa
     7381 accaccattt ataataataa tagcgccata ttctaagttg agtaaatgca tcctaccatt
     7441 caaggttatc atataattta acaaaattgt acaaccttaa ggctcttcac agtataaatt
     7501 aatttttcaa accagagctg ttcacatgaa aatattcctt agtagaatgt atatttaaga
     7561 aataaaacaa caaaggggaa ctatatcacc catgtaagtt atagagttaa ttacaagttc
     7621 taccattctt gataattctt tgagatgcta agtgtttcag ttcatattta atgatactta
     7681 aatcgtggga attttaatgt ttgttccaaa atagcagaaa ttgtaaaaat tatgaattat
     7741 tggagaaaat gccattactt gtatatctga catttaattt tttaattatg gagactacat
     7801 gcttttgtag tataatatta atgattattc tcttgtaaaa gaaaaggaaa aattagtaaa
     7861 gaaaaaagtt ttagttgatg ttacctgctt gtgagtatca attacttagt ttttaaattt
     7921 ttaaggtatt gcctactttt ctttagaatg cagaaatctc gtcctgagcc cttattatgc
     7981 ctgtggtaga gcaatgcaag actgaaaagg ttgccagtat tttcaggctt atttgactaa
     8041 gaattagtgc caagaactgg aaagtctact ttttggaggg aaatgatact tagaatttgt
     8101 aaacataagc agtaatagtg taaatattta gaaaaatagc aagtgcttta actgctcaat
     8161 aaaatagtat agatgttatt tcacccttaa ggcactggct gttgcccatt attttccgat
     8221 ttcgtgatta ctgtatatat tacatttgag taatgaataa ttggtaatag tgtattatag
     8281 tgttctacag cacaccttac tgtacagtat ttgaagtttt cttttatatc aataataaaa
     8341 ctataagtta atactgattt tcccctgaaa ttctccacat tagcttttaa aggtaatttt
     8401 atgttcttta gtttcctgga attataatgc atgaacatat agttcagttt tataaagaaa
     8461 ttcttaaata ataaagtttt cttgttgaac ttaagcgggt tatcgtacag gaatagtgat
     8521 ttttcccctt aatgtagatt tcattgctag atgaaatgga cccagtgaaa aaagagtgcg
     8581 caatttaaaa aaaaaccaaa acggtctcac tctgctgccc atgctggatt gccgtggcat
     8641 gatcagggct cactgcagcc tcaacctcct gggctcaagt gatcctccca cctcagcctc
     8701 ctgaatagct gggactacag gcatgtgcca ccacacctgg ctaatttttg tattttttgt
     8761 agagataggg ttttgccatg ttgcccaggc cggtcttgaa cccctgagct caagtgatct
     8821 gccctcctct gcctctttaa gtgctgggat tacaggcgtg agccaccgca ccctgccagc
     8881 aaaatttctt tagacatatt acataattgg gccataatgc ttactgaata tttaaaaatg
     8941 aaggcaaatt ctaacagaga acttgatttt gtggtggttt aaaaaaatac tggttagtga
     9001 taaggaaatg aaaggaaata cagataatag tactaaataa cgtttgtgta gcttagcagt
     9061 caacacgttt acttccaagt tttattgtat atgcttaata tacattttat tagatcattt
     9121 aactcaataa tttagagtat agccttagac accagactgc ctaggtctga ctcctggtac
     9181 gctgctttct gtgacctctc tgtgcattcc tctctgcgag gatgatgatc acatctgctc
     9241 ccagggtggc tgtcttaaat gagttaacac gtatctgatg attagaagag tgcctgacac
     9301 atagtaaaca ctcaaatatt tgctatgtta tatgttaatg taatgtggta gagttcagtt
     9361 gccattaagt gggataaatc ctcacagggc acacattatt taaataattt gttacattaa
     9421 aacttatttt acttgtacat aattgactaa aaaatggggg gttgtacatt caaacattta
     9481 tgaccatgtg caacactgag aatctcaaac cgacagtgtg ctctgtgacc caaggcaagt
     9541 cttggagcct ttcaaggcct caatttcttc tgtaaccttg tggtttcaca attgtcaacc
     9601 ttttgttctg ccgaccattg tcgtcatagt cttacttgca gagggcatag tgctgatggg
     9661 acaagtgaca tgggtatgag ccccatgtaa actaaaacat aattaccttt cctttaggtg
     9721 ttggctacag ttatcccaaa cttggacttg aggtcttgta tatgcatgcc attggtcaca
     9781 taccatcaga atagaattct caaaaactct ttagtaaata agataaacat cctacttata
     9841 acagctcatt atggatttta tttttattgt ctggctcttt cagaccccag caactgcaat
     9901 tctgacttaa taatcaattg tttctaatca ttaaaaatgt atatagaaaa actgaa
//
