LOCUS       NM_014000               5497 bp    mRNA    linear   PRI 20-MAR-2025
DEFINITION  Homo sapiens vinculin (VCL), transcript variant 1, mRNA.
ACCESSION   NM_014000
VERSION     NM_014000.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5497)
  AUTHORS   Solomatina,E.S., Kovaleva,A.V., Tvorogova,A.V., Vorobjev,I.A. and
            Saidova,A.A.
  TITLE     Effect of Focal Adhesion Kinase and Vinculin Expression on
            Migration Parameters of Normal and Tumor Epitheliocytes
  JOURNAL   Biochemistry (Mosc) 89 (3), 474-486 (2024)
   PUBMED   38648767
  REMARK    GeneRIF: Effect of Focal Adhesion Kinase and Vinculin Expression on
            Migration Parameters of Normal and Tumor Epitheliocytes.
REFERENCE   2  (bases 1 to 5497)
  AUTHORS   Zahavich,L., Akilen,R., George,K. and Mital,S.
  TITLE     Heart Failure with Recovered Ejection Fraction in Patients with
            Vinculin Loss-of-function Variants
  JOURNAL   J Cardiovasc Transl Res 16 (6), 1303-1309 (2023)
   PUBMED   37548861
  REMARK    GeneRIF: Heart Failure with Recovered Ejection Fraction in Patients
            with Vinculin Loss-of-function Variants.
REFERENCE   3  (bases 1 to 5497)
  AUTHORS   DeWane,G., Cronin,N.M., Dawson,L.W., Heidema,C. and DeMali,K.A.
  TITLE     Vinculin Y822 is an important determinant of ligand binding
  JOURNAL   J Cell Sci 136 (12) (2023)
   PUBMED   37248996
  REMARK    GeneRIF: Vinculin Y822 is an important determinant of ligand
            binding.
REFERENCE   4  (bases 1 to 5497)
  AUTHORS   Filatova,E.V., Krylova,N.S., Klass,A.L., Kovalevskaya,E.A.,
            Maslova,M.Y., Shadrina,M.I., Poteshkina,N.G. and Slominsky,P.A.
  TITLE     [No Effect of the p.Arg230His Variant Of The VCL Protein on the
            Course of the Hypertrophic Cardiomyopathy In Russian Family
            Carrying The p.Gln1233Ter Pathogenic Variant In The MYBPC3 Gene]
  JOURNAL   Kardiologiia 63 (3), 28-35 (2023)
   PUBMED   37061858
  REMARK    GeneRIF: [No Effect of the p.Arg230His Variant Of The VCL Protein
            on the Course of the Hypertrophic Cardiomyopathy In Russian Family
            Carrying The p.Gln1233Ter Pathogenic Variant In The MYBPC3 Gene].
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 5497)
  AUTHORS   Cirino,A.L., Channaoui,N. and Ho,C.
  TITLE     Nonsyndromic Hypertrophic Cardiomyopathy Overview
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301725
REFERENCE   6  (bases 1 to 5497)
  AUTHORS   Hershberger,R.E. and Jordan,E.
  TITLE     Dilated Cardiomyopathy Overview
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301486
REFERENCE   7  (bases 1 to 5497)
  AUTHORS   Mulligan,L.M., Gardner,E., Telenius,H. and Ponder,B.A.
  TITLE     Complementary physical and genetic techniques map the vinculin
            (VCL) gene on chromosome 10q
  JOURNAL   Genomics 13 (4), 1347-1349 (1992)
   PUBMED   1505973
REFERENCE   8  (bases 1 to 5497)
  AUTHORS   Koteliansky,V.E., Ogryzko,E.P., Zhidkova,N.I., Weller,P.A.,
            Critchley,D.R., Vancompernolle,K., Vandekerckhove,J., Strasser,P.,
            Way,M., Gimona,M. et al.
  TITLE     An additional exon in the human vinculin gene specifically encodes
            meta-vinculin-specific difference peptide. Cross-species comparison
            reveals variable and conserved motifs in the meta-vinculin insert
  JOURNAL   Eur J Biochem 204 (2), 767-772 (1992)
   PUBMED   1339348
  REMARK    Erratum:[Eur J Biochem. 1992 May 1;205(3):1218. doi:
            10.1111/j.1432-1033.1992.tb16893.x_3. PMID: 1577003]
REFERENCE   9  (bases 1 to 5497)
  AUTHORS   Weller,P.A., Ogryzko,E.P., Corben,E.B., Zhidkova,N.I., Patel,B.,
            Price,G.J., Spurr,N.K., Koteliansky,V.E. and Critchley,D.R.
  TITLE     Complete sequence of human vinculin and assignment of the gene to
            chromosome 10
  JOURNAL   Proc Natl Acad Sci U S A 87 (15), 5667-5671 (1990)
   PUBMED   2116004
REFERENCE   10 (bases 1 to 5497)
  AUTHORS   Turner,C.E. and Burridge,K.
  TITLE     Detection of metavinculin in human platelets using a modified talin
            overlay assay
  JOURNAL   Eur J Cell Biol 49 (1), 202-206 (1989)
   PUBMED   2503380
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL708715.1, BC039174.1,
            AK126978.1, AL832130.1, AA368699.1, AL700886.1, BC013847.1,
            AI290052.1 and AL731576.9.
            
            On Nov 22, 2019 this sequence version replaced NM_014000.2.
            
            Summary: Vinculin is a cytoskeletal protein associated with
            cell-cell and cell-matrix junctions, where it is thought to
            function as one of several interacting proteins involved in
            anchoring F-actin to the membrane. Defects in VCL are the cause of
            cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a
            disorder characterized by ventricular dilation and impaired
            systolic function, resulting in congestive heart failure and
            arrhythmia. Multiple alternatively spliced transcript variants have
            been found for this gene, but the biological validity of some
            variants has not been determined. [provided by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (1) differs from variant 2 in
            having an additional exon in the 3' coding region. It thus encodes
            a longer isoform (meta-VCL).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: EU794673.1, SRR14038194.2114488.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1966682, SAMEA1968189
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000211998.10/ ENSP00000211998.5
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-11                AL708715.1         3-13
            12-791              BC039174.1         1-780
            792-1076            AK126978.1         1049-1333
            1077-1655           AL832130.1         1-579
            1656-2388           AK126978.1         1913-2645
            2389-2479           AL832130.1         1313-1403
            2480-2536           AA368699.1         66-122
            2537-2905           AL832130.1         1461-1829
            2906-2939           AL700886.1         298-331
            2940-4488           AL832130.1         1864-3412
            4489-5080           AL832130.1         3415-4006
            5081-5355           BC013847.1         1413-1687
            5356-5482           AI290052.1         1-127               c
            5483-5488           AL832130.1         4412-4417
            5489-5497           AL731576.9         42963-42971
FEATURES             Location/Qualifiers
     source          1..5497
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="10"
                     /map="10q22.2"
     gene            1..5497
                     /gene="VCL"
                     /gene_synonym="CMD1W; CMH15; HEL114; MV; MVCL; VINC"
                     /note="vinculin"
                     /db_xref="GeneID:7414"
                     /db_xref="HGNC:HGNC:12665"
                     /db_xref="MIM:193065"
     exon            1..260
                     /gene="VCL"
                     /gene_synonym="CMD1W; CMH15; HEL114; MV; MVCL; VINC"
                     /inference="alignment:Splign:2.1.0"
     CDS             93..3497
                     /gene="VCL"
                     /gene_synonym="CMD1W; CMH15; HEL114; MV; MVCL; VINC"
                     /note="isoform meta-VCL is encoded by transcript variant
                     1; metavinculin; epididymis luminal protein 114;
                     meta-vinculin; epididymis secretory sperm binding protein"
                     /codon_start=1
                     /product="vinculin isoform meta-VCL"
                     /protein_id="NP_054706.1"
                     /db_xref="CCDS:CCDS7341.1"
                     /db_xref="GeneID:7414"
                     /db_xref="HGNC:HGNC:12665"
                     /db_xref="MIM:193065"
                     /translation="MPVFHTRTIESILEPVAQQISHLVIMHEEGEVDGKAIPDLTAPV
                     AAVQAAVSNLVRVGKETVQTTEDQILKRDMPPAFIKVENACTKLVQAAQMLQSDPYSV
                     PARDYLIDGSRGILSGTSDLLLTFDEAEVRKIIRVCKGILEYLTVAEVVETMEDLVTY
                     TKNLGPGMTKMAKMIDERQQELTHQEHRVMLVNSMNTVKELLPVLISAMKIFVTTKNS
                     KNQGIEEALKNRNFTVEKMSAEINEIIRVLQLTSWDEDAWASKDTEAMKRALASIDSK
                     LNQAKGWLRDPSASPGDAGEQAIRQILDEAGKVGELCAGKERREILGTCKMLGQMTDQ
                     VADLRARGQGSSPVAMQKAQQVSQGLDVLTAKVENAARKLEAMTNSKQSIAKKIDAAQ
                     NWLADPNGGPEGEEQIRGALAEARKIAELCDDPKERDDILRSLGEISALTSKLADLRR
                     QGKGDSPEARALAKQVATALQNLQTKTNRAVANSRPAKAAVHLEGKIEQAQRWIDNPT
                     VDDRGVGQAAIRGLVAEGHRLANVMMGPYRQDLLAKCDRVDQLTAQLADLAARGEGES
                     PQARALASQLQDSLKDLKARMQEAMTQEVSDVFSDTTTPIKLLAVAATAPPDAPNREE
                     VFDERAANFENHSGKLGATAEKAAAVGTANKSTVEGIQASVKTARELTPQVVSAARIL
                     LRNPGNQAAYEHFETMKNQWIDNVEKMTGLVDEAIDTKSLLDASEEAIKKDLDKCKVA
                     MANIQPQMLVAGATSIARRANRILLVAKREVENSEDPKFREAVKAASDELSKTISPMV
                     MDAKAVAGNISDPGLQKSFLDSGYRILGAVAKVREAFQPQEPDFPPPPPDLEQLRLTD
                     ELAPPKPPLPEGEVPPPRPPPPEEKDEEFPEQKAGEVINQPMMMAARQLHDEARKWSS
                     KPGIPAAEVGIGVVAEADAADAAGFPVPPDMEDDYEPELLLMPSNQPVNQPILAAAQS
                     LHREATKWSSKGNDIIAAAKRMALLMAEMSRLVRGGSGTKRALIQCAKDIAKASDEVT
                     RLAKEVAKQCTDKRIRTNLLQVCERIPTISTQLKILSTVKATMLGRTNISDEESEQAT
                     EMLVHNAQNLMQSVKETVREAEAASIKIRTDAGFTLRWVRKTPWYQ"
     exon            261..331
                     /gene="VCL"
                     /gene_synonym="CMD1W; CMH15; HEL114; MV; MVCL; VINC"
                     /inference="alignment:Splign:2.1.0"
     exon            332..482
                     /gene="VCL"
                     /gene_synonym="CMD1W; CMH15; HEL114; MV; MVCL; VINC"
                     /inference="alignment:Splign:2.1.0"
     exon            483..591
                     /gene="VCL"
                     /gene_synonym="CMD1W; CMH15; HEL114; MV; MVCL; VINC"
                     /inference="alignment:Splign:2.1.0"
     exon            592..714
                     /gene="VCL"
                     /gene_synonym="CMD1W; CMH15; HEL114; MV; MVCL; VINC"
                     /inference="alignment:Splign:2.1.0"
     exon            715..875
                     /gene="VCL"
                     /gene_synonym="CMD1W; CMH15; HEL114; MV; MVCL; VINC"
                     /inference="alignment:Splign:2.1.0"
     exon            876..966
                     /gene="VCL"
                     /gene_synonym="CMD1W; CMH15; HEL114; MV; MVCL; VINC"
                     /inference="alignment:Splign:2.1.0"
     exon            967..1114
                     /gene="VCL"
                     /gene_synonym="CMD1W; CMH15; HEL114; MV; MVCL; VINC"
                     /inference="alignment:Splign:2.1.0"
     exon            1115..1268
                     /gene="VCL"
                     /gene_synonym="CMD1W; CMH15; HEL114; MV; MVCL; VINC"
                     /inference="alignment:Splign:2.1.0"
     exon            1269..1444
                     /gene="VCL"
                     /gene_synonym="CMD1W; CMH15; HEL114; MV; MVCL; VINC"
                     /inference="alignment:Splign:2.1.0"
     exon            1445..1635
                     /gene="VCL"
                     /gene_synonym="CMD1W; CMH15; HEL114; MV; MVCL; VINC"
                     /inference="alignment:Splign:2.1.0"
     exon            1636..1835
                     /gene="VCL"
                     /gene_synonym="CMD1W; CMH15; HEL114; MV; MVCL; VINC"
                     /inference="alignment:Splign:2.1.0"
     exon            1836..1964
                     /gene="VCL"
                     /gene_synonym="CMD1W; CMH15; HEL114; MV; MVCL; VINC"
                     /inference="alignment:Splign:2.1.0"
     exon            1965..2114
                     /gene="VCL"
                     /gene_synonym="CMD1W; CMH15; HEL114; MV; MVCL; VINC"
                     /inference="alignment:Splign:2.1.0"
     exon            2115..2223
                     /gene="VCL"
                     /gene_synonym="CMD1W; CMH15; HEL114; MV; MVCL; VINC"
                     /inference="alignment:Splign:2.1.0"
     exon            2224..2526
                     /gene="VCL"
                     /gene_synonym="CMD1W; CMH15; HEL114; MV; MVCL; VINC"
                     /inference="alignment:Splign:2.1.0"
     exon            2527..2651
                     /gene="VCL"
                     /gene_synonym="CMD1W; CMH15; HEL114; MV; MVCL; VINC"
                     /inference="alignment:Splign:2.1.0"
     exon            2652..2837
                     /gene="VCL"
                     /gene_synonym="CMD1W; CMH15; HEL114; MV; MVCL; VINC"
                     /inference="alignment:Splign:2.1.0"
     exon            2838..3041
                     /gene="VCL"
                     /gene_synonym="CMD1W; CMH15; HEL114; MV; MVCL; VINC"
                     /inference="alignment:Splign:2.1.0"
     exon            3042..3245
                     /gene="VCL"
                     /gene_synonym="CMD1W; CMH15; HEL114; MV; MVCL; VINC"
                     /inference="alignment:Splign:2.1.0"
     exon            3246..3350
                     /gene="VCL"
                     /gene_synonym="CMD1W; CMH15; HEL114; MV; MVCL; VINC"
                     /inference="alignment:Splign:2.1.0"
     exon            3351..5497
                     /gene="VCL"
                     /gene_synonym="CMD1W; CMH15; HEL114; MV; MVCL; VINC"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5465..5470
                     /regulatory_class="polyA_signal_sequence"
                     /gene="VCL"
                     /gene_synonym="CMD1W; CMH15; HEL114; MV; MVCL; VINC"
                     /note="hexamer: AATAAA"
     regulatory      5480..5485
                     /regulatory_class="polyA_signal_sequence"
                     /gene="VCL"
                     /gene_synonym="CMD1W; CMH15; HEL114; MV; MVCL; VINC"
                     /note="hexamer: TATAAA"
     polyA_site      5488
                     /gene="VCL"
                     /gene_synonym="CMD1W; CMH15; HEL114; MV; MVCL; VINC"
                     /note="major polyA site"
     polyA_site      5497
                     /gene="VCL"
                     /gene_synonym="CMD1W; CMH15; HEL114; MV; MVCL; VINC"
                     /note="major polyA site"
ORIGIN      
        1 agtcgctgca cagtctgtct cttcgccggt tcccggcccc gtggatccta cttctctgtc
       61 gcccgcggtt cgccgccccg ctcgccgccg cgatgccagt gtttcatacg cgcacgatcg
      121 agagcatcct ggagccggtg gcacagcaga tctcccacct ggtgataatg cacgaggagg
      181 gcgaggtgga cggcaaagcc attcctgacc tcaccgcgcc cgtggccgcc gtgcaggcgg
      241 ccgtcagcaa cctcgtccgg gttggaaaag agactgttca aaccactgag gatcagattt
      301 tgaagagaga tatgccacca gcatttatta aggttgagaa tgcttgcacc aagcttgtcc
      361 aggcagctca gatgcttcag tcagaccctt actcagtgcc tgctcgagat tatctaattg
      421 atgggtcaag gggcatcctc tctggaacat cagacctgct ccttaccttc gatgaggctg
      481 aggtccgtaa aattattaga gtttgcaaag gaattttgga atatcttaca gtggcagagg
      541 tggtggagac tatggaagat ttggtcactt acacaaagaa tcttgggcca ggaatgacta
      601 agatggccaa gatgattgac gagagacagc aggagctcac tcaccaggag caccgagtga
      661 tgttggtgaa ctcgatgaac accgtgaaag agttgctgcc agttctcatt tcagctatga
      721 agatttttgt aacaactaaa aactcaaaaa accaaggcat agaggaagct ttaaaaaatc
      781 gcaattttac tgtagaaaaa atgagtgctg aaattaatga gataattcgt gtgttacaac
      841 tcacctcttg ggatgaagat gcctgggcca gcaaggacac tgaagccatg aagagagcat
      901 tggcctccat agactccaaa ctgaaccagg ccaaaggttg gctccgtgac cctagtgcct
      961 ccccagggga tgctggtgag caggccatca gacagatctt agatgaagct ggaaaagttg
     1021 gtgaactctg tgcaggcaaa gaacgcaggg agattctggg aacttgcaaa atgctagggc
     1081 agatgactga tcaagtggct gacctccgtg ccagaggaca aggatcctca ccggtggcca
     1141 tgcagaaagc tcagcaggta tctcagggtc tggatgtgct cacagcaaaa gtggaaaatg
     1201 cagctcgcaa gctggaagcc atgaccaact caaagcagag cattgcaaag aagatcgatg
     1261 ctgctcagaa ctggcttgca gatccaaatg gtggaccgga aggagaagag cagattcgag
     1321 gtgctttggc tgaagctcgg aaaatagcag aattatgtga tgatcctaaa gaaagagatg
     1381 acattctacg ttcccttggg gaaatatctg ctctgacttc taaattagca gatctacgaa
     1441 gacaggggaa aggagattct ccagaggctc gagccttggc caaacaggtg gccacggccc
     1501 tgcagaacct gcagaccaaa accaaccggg ctgtggccaa cagcagaccg gccaaagcag
     1561 ctgtacacct tgagggcaag attgagcaag cacagcggtg gattgataat cccacagtgg
     1621 atgaccgtgg agtcggtcag gctgccatcc gggggcttgt ggccgaaggg catcgtctgg
     1681 ctaatgttat gatggggcct tatcggcaag atcttctcgc caagtgtgac cgagtggacc
     1741 agctgacagc ccagctggct gacctggctg ccagagggga aggggagagt cctcaggcac
     1801 gagcacttgc atctcagctc caagactcct taaaggatct aaaagctcgg atgcaggagg
     1861 ccatgactca ggaagtgtca gatgttttca gcgataccac aactcccatc aagctgttgg
     1921 cagtggcagc cacggcgcct cctgatgcgc ctaacaggga agaggtattt gatgagaggg
     1981 cagctaactt tgaaaaccat tcaggaaagc ttggtgctac ggccgagaag gcggctgcgg
     2041 ttggtactgc taataaatca acagtggaag gcattcaggc ctcagtgaag acggcccgag
     2101 aactcacacc ccaggtggtc tcggctgctc gtatcttact taggaaccct ggaaatcaag
     2161 ctgcttatga acattttgag accatgaaga accagtggat cgataatgtt gaaaaaatga
     2221 cagggctggt ggacgaagcc attgatacca aatctctgtt ggatgcttca gaagaagcaa
     2281 ttaaaaaaga cctggacaag tgcaaggtag ctatggccaa cattcagcct cagatgctgg
     2341 ttgctggggc aaccagtatt gctcgtcggg ccaaccggat cctgctggtg gctaagaggg
     2401 aggtggagaa ttccgaggat cccaagttcc gtgaggctgt gaaagctgcc tctgatgaat
     2461 tgagcaaaac catctccccg atggtgatgg atgcaaaagc tgtggctgga aacatttccg
     2521 accctggact gcaaaagagc ttcctggact caggatatcg gatcctggga gctgtggcca
     2581 aggtcagaga agccttccaa cctcaggagc ctgacttccc gccgcctcca ccagaccttg
     2641 aacaactccg actaacagat gagcttgctc ctcccaaacc acctctgcct gaaggtgagg
     2701 tccctccacc taggcctcca ccaccagagg aaaaggatga agagttccct gagcagaagg
     2761 ccggggaggt gattaaccag ccaatgatga tggctgccag acagctccat gatgaagctc
     2821 gcaaatggtc cagcaagccg ggcatcccag ccgctgaggt gggtataggt gttgtagctg
     2881 aggcagatgc ggccgatgct gctggcttcc ctgtcccccc tgacatggaa gacgattacg
     2941 aacctgagct gctgttaatg ccatccaatc agccggtcaa ccagcccatt ctggccgcgg
     3001 ctcagtcctt gcatcgggaa gctaccaagt ggtctagtaa gggcaatgac atcattgcag
     3061 cagccaagcg catggctctg ctgatggctg agatgtctcg gctggtaaga gggggcagtg
     3121 gtaccaagcg ggcactcatt cagtgtgcca aggacatcgc caaggcctca gatgaggtga
     3181 ctcggttggc caaggaggtt gccaagcagt gcacagataa acggattaga accaacctct
     3241 tacaggtatg tgagcgaatc ccaaccataa gcacccagct caaaatcctg tccacagtga
     3301 aggccaccat gctgggccgg accaacatca gtgatgagga gtctgagcag gccacagaga
     3361 tgctggttca caatgcccag aacctcatgc agtctgtgaa ggagactgtg cgggaagctg
     3421 aagctgcttc aatcaaaatt cgaacagatg ctggatttac actgcgctgg gttagaaaga
     3481 ctccctggta ccagtaggca cctggctgag cctggctggc acagaaacct ctactaaaaa
     3541 gaaggaaaat gatctgagtc ccaggagctg cccagagttg ctgggagctg aaaaatcaca
     3601 tcctggcctg gcacatcaga aaggaatggg ggcctcttca aattagaaga catttatact
     3661 cttttttcat ggacactttg aaatgtgttt ctgtataaag cctgtattct caaacacagt
     3721 tacacttgtg caccctctat cccaataggc agactgggtt tctagcccat ggacttcaca
     3781 taagctcaga atccaagtga acactagcca gacactctgc tctgcccttg ttccctaggg
     3841 gacacttccc tctgtttctc tttccttggc tcccattcac tcttccagaa tcccaagacc
     3901 cagggcccag gcaaatcagt tactaagaag aaaattgctg tgcctcccaa aattgttttg
     3961 agctttccat gttgctgcca accatacctt ccttccctgg gctgtgctac ctgggtcctt
     4021 ttcagaagtg agctttgctg ctacagggga aggtggcctc tgtggagccc cagcatatgg
     4081 gggcctggat tcatttcctg cccttcctca gtttaatcct tctagtttcc cacaatataa
     4141 aactgtactt cactgtcagg aagaaatcac agaatcatat gattctgctt ttaccatgcc
     4201 cctgagcaat gtctgtgcta gggaaacttc ccgtcccata tcctgcctca gcccgccaag
     4261 gtagccatcc catgaacaca ctgtgtcctg gtgctctctg ccactggaag ggcagagtag
     4321 ccagggtgtg gccctgccat cttcccagca gggccactcc cggcactcca tgcttagtca
     4381 ctgcctgcag aggtctgtgc tgaggcctta tcattcattc ttagctctta attgttcatt
     4441 ttgagctgaa atgctgcatt ttaattttaa ccaaaacatg tctcctatcc tggtttttgt
     4501 agccttcctc cacatccttt ctaaacaaga ttttaaagac atgtaggtgt ttgttcatct
     4561 gtaactctaa aagatccttt ttaaattcag tcctaagaaa gaggagtgct tgtcccctaa
     4621 gagtgtttaa tggcaaggca gccctgtctg aaggacactt cctgcctaag ggagagtggt
     4681 atttgcagac tagaattcta gtgctgctga agatgaatca atgggaaata ctactcctgt
     4741 aattcctacc tccctgcaac caactacaac caagctctct gcatctactc ccaagtatgg
     4801 ggttcaagag agtaatgggt ttcatatttc ttatcaccac agtaagttcc tactaggcaa
     4861 aatgagaggg cagtgtttcc tttttggtac ttattactgc taagtatttc ccagcacatg
     4921 aaaccttatt ttttcccaaa gccagaacca gatgagtaaa ggagtaagaa ccttgcctga
     4981 acatccttcc ttcccaccca tcgctgtgtg ttagttccca acatcgaatg tgtacaactt
     5041 aagttggtcc tttacactca ggctttcact atttccttta taatgaggat gattattttc
     5101 aaggccctca gcatatttgt atagttgctt gcctgatata aatgcaatat taatgccttt
     5161 aaagtatgaa tctatgccaa agatcacttg ttgttttact aaagaaagat tacttagagg
     5221 aaataagaaa aatcatgttt gctctcccgg ttcttccagt ggtttgagac actggtttac
     5281 actttatgcc ggatgtgctt ttctccaata tcagtgctcg agacacagtg aagcaaatta
     5341 aaaaaaaaaa aaaaaaaaat ccctgaatga tgattagaga catcaccgct aaaaaactac
     5401 atttataagc taggatttgt tatatgcaaa tattttctgc ctcttctttt gttctgttta
     5461 aaacaataaa atgcatttgt ataaaaaatg ctttaaa
//
