LOCUS       NM_003370               2242 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens vasodilator stimulated phosphoprotein (VASP), mRNA.
ACCESSION   NM_003370
VERSION     NM_003370.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2242)
  AUTHORS   Wang,C., Zhao,X., Zhao,L., Wang,Y., Jia,Y., Zhang,X. and Ma,W.
  TITLE     PKCzeta phosphorylates VASP to mediate chemotaxis in breast cancer
            cells
  JOURNAL   Exp Cell Res 433 (2), 113823 (2023)
   PUBMED   37890607
  REMARK    GeneRIF: PKCzeta phosphorylates VASP to mediate chemotaxis in
            breast cancer cells.
REFERENCE   2  (bases 1 to 2242)
  AUTHORS   Torres-Gomez,A., Fiyouzi,T., Guerra-Espinosa,C., Cardenes,B.,
            Clares,I., Toribio,V., Reche,P.A., Cabanas,C. and Lafuente,E.M.
  TITLE     Expression of the phagocytic receptors alphaMbeta2 and alphaXbeta2
            is controlled by RIAM, VASP and Vinculin in
            neutrophil-differentiated HL-60 cells
  JOURNAL   Front Immunol 13, 951280 (2022)
   PUBMED   36238292
  REMARK    GeneRIF: Expression of the phagocytic receptors alphaMbeta2 and
            alphaXbeta2 is controlled by RIAM, VASP and Vinculin in
            neutrophil-differentiated HL-60 cells.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2242)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   4  (bases 1 to 2242)
  AUTHORS   Benz,C., Ali,M., Krystkowiak,I., Simonetti,L., Sayadi,A.,
            Mihalic,F., Kliche,J., Andersson,E., Jemth,P., Davey,N.E. and
            Ivarsson,Y.
  TITLE     Proteome-scale mapping of binding sites in the unstructured regions
            of the human proteome
  JOURNAL   Mol Syst Biol 18 (1), e10584 (2022)
   PUBMED   35044719
REFERENCE   5  (bases 1 to 2242)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   6  (bases 1 to 2242)
  AUTHORS   Reinhard,M., Jouvenal,K., Tripier,D. and Walter,U.
  TITLE     Identification, purification, and characterization of a
            zyxin-related protein that binds the focal adhesion and
            microfilament protein VASP (vasodilator-stimulated phosphoprotein)
  JOURNAL   Proc Natl Acad Sci U S A 92 (17), 7956-7960 (1995)
   PUBMED   7644520
REFERENCE   7  (bases 1 to 2242)
  AUTHORS   Reinhard,M., Giehl,K., Abel,K., Haffner,C., Jarchau,T., Hoppe,V.,
            Jockusch,B.M. and Walter,U.
  TITLE     The proline-rich focal adhesion and microfilament protein VASP is a
            ligand for profilins
  JOURNAL   EMBO J 14 (8), 1583-1589 (1995)
   PUBMED   7737110
REFERENCE   8  (bases 1 to 2242)
  AUTHORS   Haffner,C., Jarchau,T., Reinhard,M., Hoppe,J., Lohmann,S.M. and
            Walter,U.
  TITLE     Molecular cloning, structural analysis and functional expression of
            the proline-rich focal adhesion and microfilament-associated
            protein VASP
  JOURNAL   EMBO J 14 (1), 19-27 (1995)
   PUBMED   7828592
REFERENCE   9  (bases 1 to 2242)
  AUTHORS   Reinhard,M., Halbrugge,M., Scheer,U., Wiegand,C., Jockusch,B.M. and
            Walter,U.
  TITLE     The 46/50 kDa phosphoprotein VASP purified from human platelets is
            a novel protein associated with actin filaments and focal contacts
  JOURNAL   EMBO J 11 (6), 2063-2070 (1992)
   PUBMED   1318192
REFERENCE   10 (bases 1 to 2242)
  AUTHORS   Halbrugge,M., Eigenthaler,M., Polke,C. and Walter,U.
  TITLE     Protein phosphorylation regulated by cyclic nucleotide-dependent
            protein kinases in cell extracts and in intact human lymphocytes
  JOURNAL   Cell Signal 4 (2), 189-199 (1992)
   PUBMED   1319722
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BG751094.1, BC038224.1 and
            Z46389.1.
            
            On Nov 22, 2018 this sequence version replaced NM_003370.3.
            
            Summary: Vasodilator-stimulated phosphoprotein (VASP) is a member
            of the Ena-VASP protein family. Ena-VASP family members contain an
            EHV1 N-terminal domain that binds proteins containing E/DFPPPPXD/E
            motifs and targets Ena-VASP proteins to focal adhesions. In the
            mid-region of the protein, family members have a proline-rich
            domain that binds SH3 and WW domain-containing proteins. Their
            C-terminal EVH2 domain mediates tetramerization and binds both G
            and F actin. VASP is associated with filamentous actin formation
            and likely plays a widespread role in cell adhesion and motility.
            VASP may also be involved in the intracellular signaling pathways
            that regulate integrin-extracellular matrix interactions. VASP is
            regulated by the cyclic nucleotide-dependent kinases PKA and PKG.
            [provided by RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC038224.1, SRR3476690.753827.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000245932.11/ ENSP00000245932.5
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-33                BG751094.1         47-79
            34-1981             BC038224.1         1-1948
            1982-2242           Z46389.1           1940-2200
FEATURES             Location/Qualifiers
     source          1..2242
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="19"
                     /map="19q13.32"
     gene            1..2242
                     /gene="VASP"
                     /note="vasodilator stimulated phosphoprotein"
                     /db_xref="GeneID:7408"
                     /db_xref="HGNC:HGNC:12652"
                     /db_xref="MIM:601703"
     exon            1..298
                     /gene="VASP"
                     /inference="alignment:Splign:2.1.0"
     CDS             294..1436
                     /gene="VASP"
                     /codon_start=1
                     /product="vasodilator-stimulated phosphoprotein"
                     /protein_id="NP_003361.1"
                     /db_xref="CCDS:CCDS33051.1"
                     /db_xref="GeneID:7408"
                     /db_xref="HGNC:HGNC:12652"
                     /db_xref="MIM:601703"
                     /translation="MSETVICSSRATVMLYDDGNKRWLPAGTGPQAFSRVQIYHNPTA
                     NSFRVVGRKMQPDQQVVINCAIVRGVKYNQATPNFHQWRDARQVWGLNFGSKEDAAQF
                     AAGMASALEALEGGGPPPPPALPTWSVPNGPSPEEVEQQKRQQPGPSEHIERRVSNAG
                     GPPAPPAGGPPPPPGPPPPPGPPPPPGLPPSGVPAAAHGAGGGPPPAPPLPAAQGPGG
                     GGAGAPGLAAAIAGAKLRKVSKQEEASGGPTAPKAESGRSGGGGLMEEMNAMLARRRK
                     ATQVGEKTPKDESANQEEPEARVPAQSESVRRPWEKNSTTLPRMKSSSSVTTSETQPC
                     TPSSSDYSDLQRVKQELLEEVKKELQKVKEEIIEAFVQELRKRGSP"
     exon            299..470
                     /gene="VASP"
                     /inference="alignment:Splign:2.1.0"
     exon            471..636
                     /gene="VASP"
                     /inference="alignment:Splign:2.1.0"
     exon            637..721
                     /gene="VASP"
                     /inference="alignment:Splign:2.1.0"
     exon            722..771
                     /gene="VASP"
                     /inference="alignment:Splign:2.1.0"
     exon            772..1013
                     /gene="VASP"
                     /inference="alignment:Splign:2.1.0"
     exon            1014..1114
                     /gene="VASP"
                     /inference="alignment:Splign:2.1.0"
     exon            1115..1166
                     /gene="VASP"
                     /inference="alignment:Splign:2.1.0"
     exon            1167..1203
                     /gene="VASP"
                     /inference="alignment:Splign:2.1.0"
     exon            1204..1249
                     /gene="VASP"
                     /inference="alignment:Splign:2.1.0"
     exon            1250..1340
                     /gene="VASP"
                     /inference="alignment:Splign:2.1.0"
     exon            1341..1398
                     /gene="VASP"
                     /inference="alignment:Splign:2.1.0"
     exon            1399..2242
                     /gene="VASP"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2219..2224
                     /regulatory_class="polyA_signal_sequence"
                     /gene="VASP"
                     /note="hexamer: AATAAA"
     polyA_site      2242
                     /gene="VASP"
                     /note="major polyA site"
ORIGIN      
        1 acagtagtaa gagtaacact gtagccgcca ccggcaaggg gtgcgcgctg gggagcggac
       61 gctgcatccc ctttctgctg caggaacctc tcatcagacc gcctgaggga agcggcgccc
      121 ggagacccgc cccggcccgg tccacattct ccccaggaag ccggactcta tggggcggga
      181 ccctggggga gcctgagccg agcccggagc cagccccgaa cccctgaacc tccagccagg
      241 ggcgccccgg gagcagccag cccgtgggcg agccgcccgc ccgccgagca gccatgagcg
      301 agacggtcat ctgttccagc cgggccactg tgatgcttta tgatgatggc aacaagcgat
      361 ggctccctgc tggcacgggt ccccaggcct tcagccgcgt ccagatctac cacaacccca
      421 cggccaattc ctttcgcgtc gtgggccgga agatgcagcc cgaccagcag gtggtcatca
      481 actgtgccat cgtccggggt gtcaagtata accaggccac ccccaacttc catcagtggc
      541 gcgacgctcg ccaggtctgg ggcctcaact tcggcagcaa ggaggatgcg gcccagtttg
      601 ccgccggcat ggccagtgcc ctagaggcgt tggaaggagg tgggccccct ccacccccag
      661 cacttcccac ctggtcggtc ccgaacggcc cctccccgga ggaggtggag cagcagaaaa
      721 ggcagcagcc cggcccgtcg gagcacatag agcgccgggt ctccaatgca ggaggcccac
      781 ctgctccccc cgctgggggt ccacccccac caccaggacc tccccctcct ccaggtcccc
      841 ccccaccccc aggtttgccc ccttcggggg tcccagctgc agcgcacgga gcagggggag
      901 gaccaccccc tgcaccccct ctcccggcag cacagggccc tggtggtggg ggagctgggg
      961 ccccaggcct ggccgcagct attgctggag ccaaactcag gaaagtcagc aagcaggagg
     1021 aggcctcagg ggggcccaca gcccccaaag ctgagagtgg tcgaagcgga ggtgggggac
     1081 tcatggaaga gatgaacgcc atgctggccc ggagaaggaa agccacgcaa gttggggaga
     1141 aaacccccaa ggatgaatct gccaatcagg aggagccaga ggccagagtc ccggcccaga
     1201 gtgaatctgt gcggagaccc tgggagaaga acagcacaac cttgccaagg atgaagtcgt
     1261 cttcttcggt gaccacttcc gagacccaac cctgcacgcc cagctccagt gattactcgg
     1321 acctacagag ggtgaaacag gagcttctgg aagaggtgaa gaaggaattg cagaaagtga
     1381 aagaggaaat cattgaagcc ttcgtccagg agctgaggaa gcggggttct ccctgaccac
     1441 agggacccag aagacccgct tctcctttcc gcacacccgg cctgtcaccc tgctttccct
     1501 gcctctactt gacttggaat tggctgaaga ctacacagga atgcatcgtt cccactcccc
     1561 atcccacttg gaaaactcca agggggtgtg gcttccctgc tcacacccac actggctgct
     1621 gattggctgg ggaggccccc gcccttttct ccctttggtc cttcccctct gccatcccct
     1681 tggggccggt ccctctgctg gggatgcacc aatgaacccc acaggaaggg ggaaggaagg
     1741 agggaatttc acattccctt gttctagatt cactttaacg cttaatgcct tcaaagtttt
     1801 ggttttttta agaaaaaaaa atatatatat atttgggttt tgggggaaaa gggaaatttt
     1861 tttttctctt tggttttgat aaaatgggat gtgggagttt ttaaatgcta tagccctggg
     1921 cttgccccat ttggggcagc tatttaaggg gaggggatgt ctcaccgggc tgggggtgag
     1981 atatcccccc accccaggga ctccccttcc ctctggctcc ttcccctttt ctatgaggaa
     2041 ataagatgct gtaacttttt ggaacctcag ttttttgatt ttttatttgg gtaggttttg
     2101 gggtccaggc catttttttt accccttgga ggaaataaga tgagggagaa aggagaaggg
     2161 gaggaaactt ctcccctccc accttcacct ttagcttctt gaaaatgggc ccctgcagaa
     2221 taaatctgcc agtttttata aa
//
